CN113262083A

CN113262083A - Pressure reaction device and control method thereof

Info

Publication number: CN113262083A
Application number: CN202110564984.6A
Authority: CN
Inventors: 闫文强; 敖英芳; 孙牧旸; 胡晓青; 代文立
Original assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Current assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Priority date: 2021-05-24
Filing date: 2021-05-24
Publication date: 2021-08-17
Anticipated expiration: 2041-05-24
Also published as: CN113262083B

Abstract

The invention provides a pressure reaction device and a control method thereof, relating to the technical field of medical instruments, wherein the pressure reaction device comprises: a receiving portion for receiving a tissue engineering meniscal scaffold; a compression member to apply a force to the tissue engineered meniscal scaffold to compress the tissue engineered meniscal scaffold. Under the condition that the bracket bearing the tissue engineering meniscus is stressed and compressed, the tissue engineering meniscus is stressed and deformed in the stress mode of the natural meniscus, and the tissue engineering meniscus is further promoted to obtain the regional specificity and better mechanical property of the natural meniscus.

Description

Pressure reaction device and control method thereof

Technical Field

The invention relates to the technical field of medical instruments, in particular to a pressure reaction device and a control method thereof.

Background

The meniscus is a fibrous cartilage tissue of meniscus shape located between the femoral condyle and the tibial plateau, and plays roles of buffering and decompressing, absorbing shock, lubricating joints, maintaining joint stability and the like in the knee joint.

The current construction of tissue engineered menisci is mainly limited to the use of artificial or natural menisci-shaped scaffolds (e.g., polycaprolactone, PCL, silk fibroin, etc.), binding to seed cells (e.g., mesenchymal stem cells, or meniscal cells, etc.), and then adding growth factors (e.g., transforming growth factor beta, TGF-beta, fibroblast growth factor, FGF) to promote cell growth, differentiation, and collagen matrix synthesis.

However, the tissue engineering meniscus obtained by the prior art is still very different from the natural meniscus, and the articular cartilage cannot be fully protected.

Disclosure of Invention

In view of the above, the present invention provides a pressure reaction apparatus and a control method thereof to solve the above technical problems.

In a first aspect, the present application provides a pressure reaction apparatus comprising:

a receiving portion for receiving a tissue engineering meniscal scaffold;

a compression member to apply a force to an inner side of the tissue engineered meniscal scaffold to compress the tissue engineered meniscal scaffold.

The inventors of the present invention have realized that menisci have the following key complex biological characteristics:

1. in appearance, the meniscus assumes a typical wedge-shaped structure with high outside and low inside, i.e. the junction with the joint capsule, the synovial border, is higher than the medial free border;

2. in terms of blood supply, the meniscus is supplied from the branches of the internal and external arteries of the knee, and the branches form a vascular network within the joint capsule, the meniscus border is in intimate contact with the joint capsule and synovium, and the area outside the meniscus 1/3 receives blood supply from the joint capsule and synovium; whereas the medial 2/3 area lacks blood supply and provides nutrition only by synovial fluid penetration;

3. compositionally, the outer region of the meniscus is rich in type I collagen, which acts as a tension resistance, while the inner region of the meniscus mainly contains type II collagen and mucopolysaccharide, which acts as a compression resistance;

4. in the arrangement of the collagen fibers, the collagen fibers on the surface of the meniscus are in disordered arrangement, the collagen fibers are gradually in ordered arrangement from the surface to the bottom, typical circumferential fiber arrangement is presented, and radially arranged fibers are distributed among the circumferential fibers to play a role in anchoring the circumferential fibers;

5. in cell morphology, the meniscal lateral edge cells exhibit a typical fusiform fibroblast-like cell morphology, while the medial cells exhibit a rounded cartilage-like cell morphology.

Based on the above characteristics, the inventors further realized that the difference between the tissue engineered meniscus obtained in the prior art and the natural meniscus is mainly reflected in that the distribution of collagen fibers and cells does not show the regiospecificity of the natural meniscus, and the obtained tissue engineered meniscus has too great a difference in mechanical properties from the natural meniscus to sufficiently protect the articular cartilage.

To this end, the inventors have further recognized that natural menisci are under natural weight bearing conditions in which the medial free edge is more stressed and the lateral edge is more stressed, as this difference in stress patterns results in the significantly stressed free edge of the meniscus containing more collagen and mucopolysaccharides of type two and the predominantly stressed lateral edge containing more collagen of type one.

The tissue engineering meniscus scaffold is similar to the structure of a meniscus and has a wedge-shaped structure with a high outer part and a low inner part, and the inner part in the inner part mentioned in the technical scheme refers to the inner part in the outer part and the high inner part.

Therefore, the technical scheme is provided, according to the scheme, under the condition that the bracket bearing the tissue engineering meniscus is stressed and compressed, the tissue engineering meniscus is stressed and deformed in the stress mode of the natural meniscus, and the tissue engineering meniscus is further promoted to obtain the regional specificity and better mechanical property of the natural meniscus.

Preferably, the end of the compression member in contact with the tissue engineering meniscal scaffold is formed as an arc, the compression member being formed of polytetrafluoroethylene.

Because the bionic wedge-shaped structure with the high outside and the low inside is adopted by the tissue engineering meniscus support, the compression member at the arc-shaped section part is particularly beneficial to realizing the mechanical dispersion of the tissue engineering meniscus support under extrusion, and the friction force is greatly reduced by the polytetrafluoroethylene material.

Preferably, the pressure reaction device comprises:

a reaction member in which a plurality of the accommodating portions are formed;

the reaction component is arranged on the upper end surface of the platform;

and the lifting mechanism is connected with the lower end face of the platform.

Preferably, the pressure reaction apparatus further comprises:

a pressure sensor disposed on the platform and below the reaction member;

and the control mechanism is in communication connection with the pressure sensor.

Preferably, the number of the accommodating parts is multiple, and the multiple accommodating parts are arranged in an array.

Preferably, the reaction member is provided in a plurality, and a distance between two of the accommodating portions corresponding to each other in two innermost rows of the accommodating portions respectively belonging to two adjacent reaction members is 100 mm.

Preferably, the number of the reaction members is two, any one of the reaction members is formed with 12 accommodating parts, the diameter of the accommodating part is 10mm, and the distance between two adjacent accommodating parts is 26 mm.

Preferably, the pressure reaction apparatus further comprises:

the voice coil motor is in communication connection with the control mechanism and drives the compression member to lift;

the master control screen is in communication connection with the control mechanism and comprises a parameter setting interface and a pressure display interface;

and the measuring component is arranged on the platform and is used for measuring the displacement change of the compression component and the compression degree of the tissue engineering meniscus scaffold.

In a second aspect, the present application provides a method of controlling a pressure reaction apparatus, the method being for controlling a pressure reaction apparatus as described above, the method comprising:

adjusting the lifting mechanism to a first preset height, and placing the tissue engineering meniscus scaffold preloaded with the mesenchymal stem cells into the accommodating part of the reaction member;

adjusting the lift mechanism to a second predetermined height such that the compression member is in contact with the meniscal scaffold surface;

the compression frequency of the compression member is set on a parameter setting interface of the master control screen as follows: the compression head is lowered for 1 second, then maintained for 1 to 2 seconds, and then raised for 1 second; and adjusting the compressive strength such that the tissue engineered meniscal scaffold is compressed by 10% to 20%;

the culture medium and the growth factor were added to the container, and the culture environment was adjusted to a constant temperature of 37 degrees with the carbon dioxide concentration set at 5%.

Preferably, the compression frequency of the compression member is set on the parameter setting interface of the total control screen as follows: the compression head was lowered for 1 second, followed by 1 second hold, and then raised for 1 second; and adjusting the compressive strength such that the tissue engineered meniscal scaffold is compressed by 10% or 13% or 15% or 18%.

In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 shows a schematic view of an assembly drawing of a pressure reaction device;

fig. 2 shows a schematic diagram of the structure of a voice coil motor;

fig. 3 shows a schematic view of a first embodiment of a compression head;

fig. 4 shows a schematic view of the structure of a grating scale;

FIG. 5 shows a schematic diagram of a top view of a reaction member;

FIG. 6 shows a schematic diagram of the structure of an overall control screen;

fig. 7 shows a schematic view of a second embodiment of the compression head.

Reference numerals:

1-constant temperature incubator; 2-grating ruler; 21-ruler body; 22-a reading head; 23-a cable; 3-a compression head; 4-a pressure sensor; 5-a lifting platform; 6-a host; 7-a reaction building block; 71-reaction well; 8-a voice coil motor; 81-a magnet assembly; 82-a coil assembly; 9-a master control screen; 91-parameter setting interface; 92-pressure display interface.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

The pressure reaction device that this embodiment provided includes constant temperature incubator, grating chi, compression head, pressure sensor, elevating platform, host computer, reaction member, voice coil motor and total control screen.

As shown in fig. 1, fig. 1 shows a schematic view of an assembly drawing of a pressure reaction device, wherein the overall control screen 9 is not shown in fig. 1. Referring to fig. 1, a grating ruler 2, a compression head 3, a pressure sensor 4, a lifting table 5, a host machine 6, a reaction member 7 and a voice coil motor 8 are arranged inside a constant temperature incubator 1. Specifically, the main unit 6 may be formed in a plate-like structure. The plate-shaped main body 6 includes an upper end surface provided with pressure sensors 4, for example, two sets of pressure sensors 4 respectively located on the left and right sides of the upper end surface. As an example, the two sets of pressure sensors 4 may each include one pressure sensor 4. Still referring to fig. 1, as an example, the pressure sensor 4 may be embedded within the host 6 and communicatively coupled to a control mechanism described below to communicate the sensed pressure condition to the control mechanism.

As shown in fig. 1, the lower end surface of the main body 6 may be provided with a lifting table 5, and the lifting table 5 may thus adjust the height of the main body 6. As an example, the lifting platform 5 may be a hand-operated lifting platform 5 manually adjusted by using a hand-operated mode, or may be an electric lifting platform 5.

In the embodiment, two reaction members 7 are respectively disposed above the two sets of pressure sensors 4, and a specific structure of the reaction members 7 will be described below in detail with reference to fig. 5.

As shown in fig. 5, fig. 5 shows the structure of the reaction member 7, and specifically, the reaction member 7 is formed with reaction holes 71, and the diameter of the reaction holes 71 may preferably be formed to 10 mm. In an embodiment, the reaction member 7 may be formed with 12 reaction holes 71, i.e., 3 × 4 — 12 as shown in fig. 5, such that a group of 12 reaction holes 71 is provided in one reaction member 7. Further, in the present embodiment, two reaction members 7 may be provided, and are respectively provided above the two pressure sensors 4. That is, the pressure reaction apparatus in this embodiment includes 24 reaction holes 71 in two sets. Preferably, the distance between two reaction holes 71 corresponding to each other and respectively subordinate to two reaction holes 71 of two adjacent groups of reaction holes 71, of the two innermost reaction holes 71, is 100 mm.

Thus, when tissue engineering meniscal culture is performed, 24 tissue engineering meniscal cultures can be simultaneously performed corresponding to 24 reaction wells 71. Wherein the center-to-center distance of the reaction holes 71 may preferably be formed to be 26 mm. The reaction member 7 is preferably made of Polystyrene (PS), and may be made of Polyethylene (PE).

Further, referring to fig. 1 to 3, in the present embodiment, the compression heads 3 are disposed above the reaction member 7, and each compression head 3 corresponds to one of the reaction holes 71. As shown in fig. 3, the compression head 3 has a substantially columnar structure as a whole. The columnar compression head 3 includes three parts from top to bottom, including a small diameter part at the top, a large diameter part with the largest diameter at the middle, and a middle diameter part at the bottom, which are connected in sequence.

As shown in fig. 1, a plurality of compression heads 3 may be mounted on a bracket (not shown), for example, by inserting a small diameter portion into a mounting hole (not shown) formed in the bracket. In the present embodiment, the lower end of the intermediate diameter portion, i.e., the lower end of the compression head 3, has an arc shape, and such a compression head 3 will be hereinafter simply referred to as an arc-shaped compression head. The arc-shaped compression head can particularly match the wedge-shaped structure with high outside and low inside of the tissue engineering meniscus scaffold.

Furthermore, the compression head 3 in this embodiment may be formed of polytetrafluoroethylene, which has good biological stability and biocompatibility, and a very low friction coefficient, and effectively reduces the friction between the compression head 3 and the tissue engineering meniscus. Preferably, the diameter of the middle diameter portion of the compression head 3 in the present embodiment is 10 mm.

As mentioned above, the above arc-shaped compression head 3 is suitable for constructing tissue engineered menisci, where such arc-shaped compression head 3 is the first embodiment of the compression head 3 of the present embodiment. Referring to fig. 7, fig. 7 shows a second embodiment of the compression head 3 of the present embodiment, which is different from the arc-shaped compression head 3 of the first embodiment in that the lower end of the compression head 3 of the second embodiment is a plane, and such a compression head 3 can also be used for constructing tissue-engineered cartilage.

Still referring to fig. 1, on the basis of the above-described features, the above-described bracket mounting the compression head 3 is mounted to the voice coil motor 8, and the voice coil motor 8 is in communication with a control mechanism described below, and the operation principle of the voice coil motor 8 will be briefly described below. With reference to fig. 2, the voice coil motor 8 includes a magnet assembly 81 and a coil assembly 82, wherein the magnet assembly 81 can be disposed at the inner top of the incubator 1 and provide a magnetic field environment, and the coil assembly is powered on to drive the compression head 3 below to lift, so as to realize periodic pressure stimulation on the tissue engineering meniscus. The voice coil motor 8 has high control precision and is particularly suitable for controlling the stimulation pressure applied to the tissue engineering meniscus, so that the mechanical property of the obtained tissue engineering meniscus is more favorable for being close to that of a real meniscus.

Further, the linear scale 2 in the present embodiment includes a scale body 21, a reading head 22 and a cable 23. With reference to fig. 1 and 3, the ruler body 21 is vertically installed on the side of the main frame 6, the grating is installed inside the ruler body 21, the reading head 22 can be used for recording the displacement change of the compression head 3 and the compression degree of the tissue engineering meniscus scaffold, and the cable 23 is used for connecting a power supply.

In the embodiment, the pressure reaction device further comprises a control mechanism, and the master control screen 9 is in communication connection with the control mechanism, and the master control screen and the control mechanism can be arranged outside the constant temperature incubator 1, and comprise a parameter setting interface 91 and a pressure display interface 92. Wherein, the parameter setting interface 91 can be used for setting the compression frequency and the compression strength, the pressure display interface 92 can record the compression strength of each compression in real time, the compression strength of each compression is obtained by the pressure sensor 4, and the obtained pressure strength can be used for analyzing the later experimental results.

Based on the above-described features, taking the hand-operated lifting platform 5 as an example, the present embodiment also provides a control method of the pressure reaction device, which will be described below.

First, the hand-operated elevating platform 5 is set to a suitable height, for example, a first predetermined height, and the tissue engineering meniscal scaffold preloaded with mesenchymal stem cells is placed in the reaction well 71 of the reaction member 7. The compression head 3 is mounted on the voice coil motor 8 and the height of the lifter 5 is then adjusted until the compression head 3 contacts the meniscal support surface, at which time the lifter 5 may be at a second predetermined height. And setting a proper compression frequency on a parameter setting interface 91 of the overall control screen 9.

In this embodiment, the parameter frequency of 1 second down-1 second to 2 seconds up-1 second up-compression head 3 is used (i.e. the compression head 3 performs a 1 second down motion followed by 1 second to 2 seconds, preferably 1 second, and then 1 second up in the lowest position), with a strength to achieve a meniscal scaffold compression of 10% to 20%, preferably a 10% deformation, and also 13%, 15% and 18%. After the parameters are set, the culture solution and the growth factors are added into the reaction holes 71, the temperature of the incubator is set to be constant 37 ℃, and the concentration of carbon dioxide is set to be 5%. Finally, under the double stimulation of growth factor chemical factors and mechanics, the tissue engineering meniscus which is more bionic in composition, structure and mechanical properties is realized.

In this embodiment, the compression head 3 can simulate the femoral condyle cartilage in vivo, and can make the meniscus support generate a certain deformation after moving downwards, thereby simulating the deformation of the meniscus generated by extrusion under the condition of bearing a load. And because of the bionic high-outside and low-inside wedge-shaped structure of the tissue engineering meniscus scaffold, the mechanical dispersion is realized under the extrusion of the arc-shaped compression head 3, so that the inner part of the meniscus scaffold is mainly under pressure, and the outer edge of the scaffold is mainly under tension, which is similar to the mechanical characteristics of the natural meniscus. This mechanical differential distribution can lead to differences in cell phenotype in different regions of the meniscal scaffold, where cells dominated by stress exhibit a chondroid cell phenotype, producing two-type collagen and mucopolysaccharide, which are the major components of the inner side of the natural meniscus. Cells mainly stressed by tension generate collagen which is a main component at the outer side of the natural meniscus, and the tissue engineering meniscus component and mechanical property partition are realized. Finally, the constructed tissue engineering meniscus is more similar to a natural meniscus in composition, collagen arrangement and mechanical properties.

The above description is only for the preferred embodiment of the present invention and is not intended to limit the scope of the present invention, and all the modifications and equivalents of the present invention as described in the specification and drawings or directly/indirectly applied to other related technical fields are included in the scope of the present invention.

Claims

1. A pressure reaction device, comprising:

a receiving portion for receiving a tissue engineering meniscal scaffold;

2. The pressure reaction device of claim 1 wherein the end of the compression member in contact with the tissue engineered meniscal scaffold is formed as a curved surface, the compression member being formed of polytetrafluoroethylene.

3. The pressure reactor device according to claim 1, wherein the pressure reactor device comprises:

the reaction component is arranged on the upper end surface of the platform;

and the lifting mechanism is connected with the lower end face of the platform.

4. The pressure reactor apparatus of claim 3, further comprising:

a pressure sensor disposed on the platform and below the reaction member;

5. The pressure reaction device according to claim 3, wherein the number of the receiving portions is plural, and the plural receiving portions are arranged in an array.

6. A pressure reaction apparatus according to claim 5, wherein the reaction member is plural in number, and a distance between two of the accommodating portions corresponding to each other in two innermost columns of the accommodating portions respectively belonging to adjacent two of the reaction members is 100 mm.

7. A pressure reaction device according to claim 6, wherein the number of the reaction members is two, any one of the reaction members is formed with 12 receiving portions, the diameter of the receiving portion is 10mm, and the distance between adjacent two receiving portions is 26 mm.

8. The pressure reactor apparatus of claim 4, further comprising:

9. A method for controlling a pressure reaction apparatus, the method being used for controlling the pressure reaction apparatus according to claim 8, the method comprising:

10. The method of claim 9,

the compression frequency of the compression member is set on a parameter setting interface of the master control screen as follows: the compression head was lowered for 1 second, followed by 1 second hold, and then raised for 1 second; and adjusting the compressive strength such that the tissue engineered meniscal scaffold is compressed by 10% or 13% or 15% or 18%.