CN113249514B - Cucumber female line character related SCAR marker and application thereof - Google Patents

Cucumber female line character related SCAR marker and application thereof Download PDF

Info

Publication number
CN113249514B
CN113249514B CN202110721114.5A CN202110721114A CN113249514B CN 113249514 B CN113249514 B CN 113249514B CN 202110721114 A CN202110721114 A CN 202110721114A CN 113249514 B CN113249514 B CN 113249514B
Authority
CN
China
Prior art keywords
cucumber
female line
female
marker
screening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110721114.5A
Other languages
Chinese (zh)
Other versions
CN113249514A (en
Inventor
杨宗辉
曹齐卫
孟昭娟
李利斌
陈伟
高天
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vegetable Research Institute of Shandong Academy of Agricultural Sciences
Original Assignee
Vegetable Research Institute of Shandong Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vegetable Research Institute of Shandong Academy of Agricultural Sciences filed Critical Vegetable Research Institute of Shandong Academy of Agricultural Sciences
Priority to CN202110721114.5A priority Critical patent/CN113249514B/en
Publication of CN113249514A publication Critical patent/CN113249514A/en
Application granted granted Critical
Publication of CN113249514B publication Critical patent/CN113249514B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a cucumber female line character related SCAR marker and application thereof, wherein the marker is positioned in a promoter region of a coding cucumber ethylene synthetase gene CsACS1G on a No. 6 chromosome, female line breeding can be carried out by utilizing the SCAR marker, the breeding time can be effectively shortened, the material planting area is reduced, and the breeding process of the female line is greatly accelerated. The method replaces the traditional field phenotype observation and identification method, can be used for identifying in seedling stage and indoors, is more rapid and efficient than the method for identifying in field phenotype observation in flowering stage, is suitable for screening breeding materials on a large scale, and can save land and labor cost.

Description

Cucumber female line character related SCAR marker and application thereof
Technical Field
The invention relates to a cucumber female line character related SCAR marker and application thereof, belonging to the field of molecular biology.
Background
Cucumber is one of the major vegetable crops in the world, according to the statistics of a world food and agriculture organization public database (FAOSTAT), the cultivation area and the yield of the cucumber in China in 2019 reach 125.8 million hectares and 7033.9 million tons, which respectively account for 56.4 percent and 80 percent of the total amount of the world, and the significance of the cucumber in the production of the China is highlighted. The main cultivated species currently produced in China is the North China stichopus japonicus selenka type cucumber, and the yield and the quality of the cucumber are limited by the number of female flowers. The female line variety has more female flowers and more melons, so that the yield can be effectively improved, meanwhile, the emasculation is not needed, the seed production procedure can be greatly simplified under the condition of ensuring the purity of the hybrid seeds, and the efficiency and the yield of fine breed breeding can be effectively improved. However, female line varieties bred in the current production are mostly bred through multi-generation backcross transformation, the breeding period is long, time and labor are wasted, partial female line materials are influenced by the environment, and the field selection accuracy is limited. The existence of the problems greatly restricts the screening of cucumber female line materials and the breeding of female line varieties.
Sequence-specific amplified region (SCAR) is a technology for specifically amplifying a certain specific fragment by designing a primer on the basis of sequence sequencing, and has the characteristics of rapidness, simplicity and low cost in application due to strict PCR reaction conditions, stable result and strong repeatability, so that the SCAR becomes a preferred marker in various molecular markers. The female line material widely used in the production at present is mainly a material with an F gene locus, the conservation of the F gene determining the female line character is caused by 1-3 CsACS1G gene copies caused by the repetition of a segment of 30.2kb of chromosome 6, and the unequal exchange of CsACS1G region CNV influences the stability of the female line. Therefore, the efficient and stable SCAR marker can be developed in the promoter region of the CsACS1G gene recombination, is used for screening female line materials and auxiliary selection of backcross transformation, can effectively shorten breeding time, reduce material planting area, greatly accelerate the breeding process of female lines, and has great production value.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides the SCAR marker related to the cucumber female line character and the application thereof, the marker is positioned in the promoter region of the coding cucumber ethylene synthetase gene CsACS1G on the No. 6 chromosome, female line breeding can be carried out by utilizing the SCAR marker, the breeding time can be effectively shortened, the material planting area can be reduced, and the breeding process of the female line can be greatly accelerated.
An SCAR marker related to cucumber female line traits, wherein the sequence of the SCAR marker is shown as SEQ ID NO. 3.
The method for screening the cucumber female line material by the SCAR marker related to the cucumber female line character comprises the following steps:
1) Extracting the genome DNA of the cucumber to be detected;
2) Taking the genome DNA of the cucumber to be detected as a template, and carrying out PCR amplification reaction by using a marker primer;
3) Detecting the amplification product, marking the amplification result to judge the variety to be identified, wherein the material with 356bp band is female material, and the material without band is non-female material.
Further, the labeled primer in the step 2) comprises an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is shown as SEQ ID No.1, and the nucleotide sequence of the downstream primer is shown as SEQ ID No. 2.
Further, the reaction system of the PCR amplification reaction in step 2) is 20 μ l, which is as follows:
mu.l of 10 ng/. Mu.l template DNA, 1. Mu.l of 10 pmol/. Mu.l upstream primer, 1. Mu.l of 10 pmol/. Mu.l downstream primer, 10. Mu.l of 2X M5HiPer plus taq HiFi PCR mix, and 6. Mu.l ultrapure water.
Further, the amplification procedure of the PCR amplification reaction in step 2) above is:
pre-denaturation at 95 ℃ for 1min; then denaturation at 95 ℃ is 15s, annealing at 55 ℃ is 15s, extension at 72 ℃ is 30s, and 30 cycles are counted; finally, extension is carried out for 10min at 72 ℃.
The SCAR marker related to the cucumber female line character is applied to screening of cucumber female line materials and auxiliary selection of backcross transformation.
The cucumber female line character related SCAR marker is applied to the cucumber molecular marker assisted breeding.
Has the beneficial effects that:
(1) The melon female line character related SCAR marker can be used for female line breeding, the method replaces the traditional field phenotype observation and identification method, identification can be carried out in seedling stage and indoor, and the method is quicker and more efficient than the method that identification is carried out in flowering stage through phenotype observation and identification in field, is suitable for large-scale screening of breeding materials, and can save land and labor cost.
(2) The marker is a dominant marker, 12 single plants can be selected for marker detection in the seedling stage of each strain of the early generation, and the homozygous lines which are all provided with strips can be considered to be planted in the field. The identification method has the theoretical accuracy rate of about 97 percent, is greatly higher than that of the identification method for field phenotype observation, can effectively shorten the breeding time, reduce the planting area of materials and accelerate the breeding process of cucumber female line varieties
Drawings
FIG. 1 shows the electrophoretic patterns of different cucumber material markers.
FIG. 2 shows the electrophoretic patterns of F3 family markers derived from the crossing of female and non-female lines.
Detailed Description
In order to make the technical solutions in the present application better understood, the present invention is further described below with reference to examples, which are only a part of examples of the present application, but not all examples, and the present invention is not limited by the following examples.
Example 1
Planting 14 North China type materials and 14 south China type materials in the field respectively, taking leaves at the seedling stage to extract DNA, then carrying out PCR amplification and molecular marker detection analysis, and investigating the sex type of each material at the flowering stage. The method comprises the following specific steps:
1) DNA extraction:
1. taking cucumber leaves with proper size of 1cm 2;
2. adding steel balls and 500. Mu.L of extraction buffer (12.1g of Tris,28.1g of NaCl,18.6g of EDTA, adding water to a constant volume of 1L), grinding for 3min, and centrifuging at 12000rpm for 10min;
3. sucking 400-450 μ L of supernatant to 1.5mL centrifuge tube, adding equal volume of isopropanol (or 2 times volume of glacial ethanol) for precipitation, standing at-20 deg.C for about 30min, and centrifuging for 5min;
4. pouring off ethanol, adding 500 μ L of 75% ethanol, soaking and washing for 10min, pouring off 75% ethanol, and washing repeatedly for 1 time;
5. after air drying, adding 100 mu L ddH2O to dissolve for about 1h to obtain the cucumber sample DNA solution.
2) And (3) PCR amplification:
taking the prepared cucumber sample DNA solution for PCR amplification, wherein the nucleotide sequence of an upstream primer is shown as SEQ ID NO.1, the nucleotide sequence of a downstream primer is shown as SEQ ID NO.2, and the reaction system of the PCR amplification reaction is 20 mu l, which is specifically as follows:
mu.l of 10 ng/. Mu.l template DNA, 1. Mu.l of 10 pmol/. Mu.l upstream primer, 1. Mu.l of 10 pmol/. Mu.l downstream primer, 10. Mu.l of 2X M5HiPer plus taq HiFi PCR mix, and 6. Mu.l ultrapure water.
PCR amplification procedure: pre-denaturation at 95 ℃ for 1min; then denaturation at 95 ℃ is carried out for 15s, annealing at 55 ℃ is carried out for 15s, extension at 72 ℃ is carried out for 30s, and 30 cycles are counted; finally, extension is carried out for 10min at 72 ℃.
3) And (3) detecting the amplification result of the product:
the PCR amplified product was loaded on 1% agarose gel containing gelred nucleic acid dye at a concentration of 1% by mass, electrophoresed for 15min at a constant voltage of 180V, and the labeling results were archived and analyzed by photographing using a gel imaging system, and the results are shown in FIG. 1. The Marker used in FIG. 1 is DL2000, 4 bands are shown in the figure, the sizes are 750bp,500bp,250bp and 100bp in sequence, the band corresponding to the amplified product is 356bp, and the band with 356bp is female line.
The upper panel in fig. 1 is the result of amplification of the marker in a south china type cucumber, where female line material is: 1, new jade star; the upper panel shows the results of the amplification of the marker in a south China cucumber type, where the female line material is: 1, new jade star; 2, grinding into jade; 3, carrying out blue grinding on the mixture to obtain 1;5, carrying out blue and white grinding for 2;7, grinding the mixture into powder; 8, grinding the mixture into 4 parts; 9, grinding the mixture into 5 parts; 10, grinding the mixture into 6 parts; 13, grinding the mixture into powder and 7. The non-female material is 4, and the wild white fur of the Haiyang is 1;6, breeding 2 places of the white skin of the Haiyang; 11, local breeding of the cucumber in Laisiidiu 1;12, local species of the cucumber in Laisiidiu 2;14, local species of cucumber leixi di 3.
The lower panel in fig. 1 is the result of amplification of the marker in north China Spanish type cucumber, where the female line material is: 1, kerun 99;3, jin you 385;4, jin you 626;5, dride 10;6, bomei 8;11, bomei 9;12, de-let L108;13, jin Sheng 103. Non-female line materials were: 2, luhuang No. 9; 7, 1-2 parts of wintersweet; 8, 1-3 parts of wintersweet; 9, jin you 35;10, zhongnong No. 26; 14, xintaimi thorn.
Example 2
In order to further verify the effectiveness of the method, the method is adopted to detect the F3 families derived by the hybridization of female lines and non-female lines in the transformation process of 6 female line materials planted in the field, each family detects 12 single plants, and the genotype of the family is judged according to the detection result. The method comprises the following specific steps:
1) DNA extraction:
1. taking cucumber leaves with proper size of 1cm 2;
2. adding steel balls and 500 μ L of extraction buffer (12.1g Tris,28.1g NaCl,18.6g EDTA, adding water to a constant volume of 1L), grinding for 3min, and centrifuging at 12000rpm for 10min;
3. sucking 400-450 μ L of supernatant into a 1.5mL centrifuge tube, adding equal volume of isopropanol (or 2 times volume of glacial ethanol) for precipitation, standing at-20 deg.C for about 30min, and centrifuging for 5min;
4. pouring off ethanol, adding 500 μ L of 75% ethanol, soaking and washing for 10min, pouring off 75% ethanol, and washing repeatedly for 1 time;
5. after air drying, 100 mu L ddH2O is added to dissolve for about 1h, and then the cucumber sample DNA solution can be obtained.
2) And (3) PCR amplification:
taking the prepared cucumber sample DNA solution for PCR amplification, wherein the nucleotide sequence of an upstream primer is shown as SEQ ID NO.1, the nucleotide sequence of a downstream primer is shown as SEQ ID NO.2, and the reaction system of the PCR amplification reaction is 20 mu l, which is specifically as follows:
mu.l of 10 ng/. Mu.l template DNA, 1. Mu.l of 10 pmol/. Mu.l upstream primer, 1. Mu.l of 10 pmol/. Mu.l downstream primer, 10. Mu.l of 2X PCRMIX, and 6. Mu.l ultrapure water.
PCR amplification procedure: pre-denaturation at 95 ℃ for 1min; then denaturation at 95 ℃ is 15s, annealing at 55 ℃ is 15s, extension at 72 ℃ is 30s, and 30 cycles are counted; finally, extension is carried out for 10min at 72 ℃.
3) And (3) detecting the amplification result of the product:
the PCR amplified product was loaded on 1% agarose gel containing gelred nucleic acid dye at a concentration of 1% by mass, electrophoresed for 15min at a constant voltage of 180V, and the labeling results were archived and analyzed by photographing using a gel imaging system, and the results are shown in FIG. 2. In FIG. 2: the Marker is DL2000, 6 bands are shared in the figure, the sizes are 2000bp,1000bp,750bp,500bp,250bp and 100bp in sequence, the band corresponding to the amplification product is 356bp,12 single plants all have the band and are considered as homozygous female line, 12 single plants all do not have the band and are considered as non-female line, and in other cases, the heterozygous female line is adopted
The three families A, D and E in FIG. 2 are heterozygote female lines
Two families B and C in FIG. 2 are homozygous female lines
Family F is a non-female line.
SEQUENCE LISTING
<110> institute of vegetables and flowers of academy of agricultural sciences of Shandong province
<120> cucumber female line character related SCAR marker and application thereof
<130> 2021
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 20
<212> DNA
<213> Artificial sequence
<400> 1
ggaaagtgtc tccgaccaga 20
<210> 2
<211> 20
<212> DNA
<213> Artificial sequence
<400> 2
tatctaggct gccactggag 20
<210> 3
<211> 356
<212> DNA
<213> sequence
<400> 3
tatctaggct gccactggag cgtgccctca ctgaacaaaa tcaaactggc ctattttcct 60
ttcttttttt gtttttcatt aaaataatca ctccttcatt gatatgcaac ttagcaaaat 120
atataaatac tagataagaa aacccaaatc aagagaagca acgacaaaaa cagggtggtt 180
taagtattta actacttcgg acgggcatag ttatgagcaa cattaattat gttttcccct 240
aacaaattaa cccccaaatc attaacattt ttcattatcc cattcatttt tgtttcataa 300
tccccatttt catcttccat atcaaaccat acctgctctg gtcggagaca ctttcc 356

Claims (4)

1. A method for screening cucumber female line material by SCAR marker related to cucumber female line character is characterized by comprising the following steps:
1) Extracting the genome DNA of the cucumber to be detected;
2) Taking the genome DNA of the cucumber to be detected as a template, and carrying out PCR amplification reaction by using a labeled primer; the labeled primers comprise an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown in SEQ ID No.1, and the nucleotide sequence of the downstream primer is shown in SEQ ID No. 2;
3) Detecting the amplification product, marking the amplification result to judge the variety to be identified, wherein the material with 356bp bands is a female material, and the material without bands is a non-female material; the sequence of the SCAR marker is shown as SEQ ID NO. 3.
2. The method for screening female cucumber line material as claimed in claim 1, wherein the reaction system of the PCR amplification reaction in step 2) is 20 ul, and specifically the following:
mu.l of 10 ng/. Mu.l template DNA, 1. Mu.l of 10 pmol/. Mu.l upstream primer, 1. Mu.l of 10 pmol/. Mu.l downstream primer, 10. Mu.l of 2X M5HiPer plus taqHiFi PCR mix, and 6. Mu.l ultrapure water.
3. The method for screening cucumber female line material as claimed in claim 1, wherein the amplification procedure of the PCR amplification reaction in step 2) is as follows:
pre-denaturation at 95 ℃ for 1min; then denaturation at 95 ℃ is 15s, annealing at 55 ℃ is 15s, extension at 72 ℃ is 30s, and 30 cycles are counted; finally, extension is carried out for 10min at 72 ℃.
4. Use of primers for SCAR markers related to cucumber female line traits as defined in claim 1 for screening cucumber female line material and for assisted selection for backcross transformation.
CN202110721114.5A 2021-06-28 2021-06-28 Cucumber female line character related SCAR marker and application thereof Active CN113249514B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110721114.5A CN113249514B (en) 2021-06-28 2021-06-28 Cucumber female line character related SCAR marker and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110721114.5A CN113249514B (en) 2021-06-28 2021-06-28 Cucumber female line character related SCAR marker and application thereof

Publications (2)

Publication Number Publication Date
CN113249514A CN113249514A (en) 2021-08-13
CN113249514B true CN113249514B (en) 2022-10-14

Family

ID=77189892

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110721114.5A Active CN113249514B (en) 2021-06-28 2021-06-28 Cucumber female line character related SCAR marker and application thereof

Country Status (1)

Country Link
CN (1) CN113249514B (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101220391A (en) * 2007-09-27 2008-07-16 东北农业大学 Technique for authenticating cucumber female series with cs-acslg special gene mark
CN104313020B (en) * 2014-10-15 2017-10-10 广东省农业科学院蔬菜研究所 Indel molecular labelings and its application with hua ' nan-type cucumber gynoecy shape close linkage
CN109207574B (en) * 2018-11-21 2021-07-20 山东省农业科学院蔬菜花卉研究所 Cucumber female SNP molecular marker and application thereof

Also Published As

Publication number Publication date
CN113249514A (en) 2021-08-13

Similar Documents

Publication Publication Date Title
CN103205500B (en) A kind of quick analysis and the multi-color fluorescence in situ hybridization method of qualification Semen Tritici aestivi exogenous chromosome
CN106916897B (en) Molecular marker for identifying purity of pumpkin hybrid seeds &#39;Yinhui No. three&#39; of Indian pumpkin and application of molecular marker
CN113151553A (en) Molecular marker coseparated with gene Clbl of watermelon plant few lateral branches and application
CN107022634A (en) A kind of molecule labelling method for differentiating rice ear sprouting period gene qHD7.4
CN114736979A (en) SNP locus for detecting watermelon full-flange leaf shape, closely linked molecular marker and application
CN107058518B (en) SSR molecular marker closely linked with sesame stem blight-resistant major gene locus and application thereof
CN106755413B (en) Rice nitrogen absorption and utilization site qNUE6 and molecular marking method thereof
CN113151550A (en) Molecular marker CmSSR02 closely linked with main effect QTL fft2 of early flowering characteristics of melons and application thereof
CN110331222B (en) Molecular marker related to cotton fertility restoration and application thereof
CN113249514B (en) Cucumber female line character related SCAR marker and application thereof
CN114752702B (en) Molecular marker BnCa-2C2 closely linked with rape calcium content trait QTL and application thereof
CN116732219A (en) Method for identifying variety of F1 generation by interspecific hybridization of fraxinus mandshurica and white wax
CN116240207A (en) Primer group for identifying Sanhua plums and early plums and application thereof
CN113832251B (en) SNP locus combination for detecting tomato mosaic virus resistance and application thereof
CN113637791B (en) Molecular marker for simultaneously identifying restorability and authenticity of pepper male sterile three-line hybrid and identification method thereof
CN111172317B (en) Molecular marker HSRC3911 closely linked with major QTL site in flowering phase of sesame and application thereof
CN110643728B (en) Method for improving breeding efficiency of poplar crossbreeding
CN111485032A (en) Method for identifying cucumber female line and SNP primer combination used by same
CN101565748A (en) Molecular labeling method for quickly identifying pear plants with self-fertility
CN111004857A (en) Molecular marker primer of major QTL (quantitative trait locus) site of soybean branch number and application of molecular marker primer
CN117363785B (en) Method for screening or assisting in screening wheat with different grain lengths and special primer group thereof
CN117535442B (en) KASP (KASP-based sequence analysis) mark for identifying wheat precipitation value and application
CN113699273B (en) SNP locus combination for detecting resistance of tomato root-knot nematode and application thereof
CN114634991B (en) InDel marker for identifying high-variety coconuts and application thereof
CN112342309B (en) SNP molecular marker related to cotton flower basal leaf spot character and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant