CN113248585B - Wheat TaARF25-B gene, mutant thereof and application thereof in regulating plant height and grain traits - Google Patents
Wheat TaARF25-B gene, mutant thereof and application thereof in regulating plant height and grain traits Download PDFInfo
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
Abstract
The invention relates to the technical field of plant genetic engineering, in particular to a wheat TaARF25-B gene, a mutant thereof and application thereof in regulating plant height and grain traits. The invention provides a wheat Taarf25-B mutant, which can reduce the plant height, grain size and grain weight of wheat. The invention also finds that the wheat TaARF25-B gene has the function of regulating and controlling plant height, grain size and grain weight, and can reduce plant height, grain size (grain length and grain width) and grain weight (thousand grain weight) by inhibiting expression of the wheat TaARF25-B gene. The Taarf25-B mutant, the TaARF25-B gene and the functions thereof provided by the invention have good application values, and provide important gene resources and methods for improving the plant type, the seed character and the yield of wheat.
Description
Technical Field
The invention relates to the technical field of plant genetic engineering, in particular to a wheat TaARF25-B gene, a mutant thereof and application thereof in regulating plant height and seed traits.
Background
Wheat (Triticum aestivum L) is one of the most important food crops in the world, and the level of the yield directly affects the living standard of people. Wheat yield is composed of the number of ears per unit area, the number of grains per ear and the weight of thousand grains. The grain size and the filling degree determine the thousand-grain weight of the wheat; the grain length, the grain width and the grain thickness determine the final grain size of the wheat, and the grain size is an important character selected in modern breeding. In addition, the plant height is also an important agronomic character of the morphogenesis and yield formation of the wheat, and the appropriate plant height can reduce the lodging incidence rate, increase the grain number per spike and improve the harvest index, thereby improving the grain yield and quality.
Auxins are involved in regulating many biological processes and play an important role in the growth and development of plants. The auxin signaling pathway consists of the AUX/IAA repressor gene, the auxin receptor (TIR1), the Auxin Response Factor (ARF) and downstream target genes. The function report research on the wheat ARFs gene is less, so that the method has important significance for separating the yield-related auxin pathway gene in wheat.
Disclosure of Invention
The invention aims to provide a wheat TaARF25-B gene, a coding protein thereof, a mutant thereof and application thereof in regulating plant height and grain traits.
According to the invention, two tetraploid mutants kronos824 and kronos2217 with plant height and grain related characters are obtained by constructing and screening a wheat EMS mutant library, and sequence analysis is carried out on the mutants to find that the Taarf25-B mutant has the function of regulating and controlling the plant height and grain size of wheat, so that the plant height, the grain size and the grain weight can be remarkably reduced, and further, the wheat TaARF25-B protein has the function of regulating and controlling the plant height and the grain size of wheat.
Specifically, the invention provides the following technical scheme:
in a first aspect, the invention provides a wheat Taarf25-B mutant, which takes an amino acid sequence of a wild-type TaARF25-B protein of wheat as a reference sequence, and the wheat Taarf25-B mutant lacks the amino acids from 336 th position to 892 th position or lacks the amino acids from 686 th position to 892 th position.
Specifically, the wheat Taarf25-B mutant has any one of the following amino acid sequences:
(1) an amino acid sequence shown as SEQ ID NO.1 or SEQ ID NO. 2;
(2) the amino acid sequence of the protein with the same function is obtained by replacing, deleting or inserting one or more amino acids in the amino acid sequence shown in SEQ ID NO.1 or SEQ ID NO. 2.
In a second aspect, the invention provides a gene encoding said wheat Taarf25-B mutant.
Specifically, the nucleotide sequence of the gene for coding the wheat Taarf25-B mutant is shown as SEQ ID NO.5 or SEQ ID NO. 6.
In addition to the nucleotide sequences of the genes encoding the wheat Taarf25-B mutants provided above, the skilled person can use codons suitable for a particular species as required in view of codon degeneracy and codon preference of different species, and all genes encoding the wheat Taarf25-B mutants are within the scope of the present invention.
In a third aspect, the invention provides a biological material comprising said gene, said biological material being an expression cassette, a vector or a host cell.
Wherein the vector comprises an expression vector and a cloning vector. The host cell comprises a microbial cell or a plant cell (preferably a non-propagating plant cell).
In a fourth aspect, the invention provides an application of the wheat Taarf25-B mutant or the coding gene thereof or the biological material containing the coding gene thereof in regulating and controlling plant height, grain size, grain weight or yield of plants.
The invention also provides application of the wheat Taarf25-B mutant or the coding gene thereof or the biological material containing the coding gene thereof in germplasm resource improvement, genetic breeding or transgenic plant construction of plant height, grain size, grain weight or yield.
In the invention, the grain size is preferably the grain length and the grain width of the grain, and the weight of the grain can be reflected by the parameter of thousand grain weight.
The above use can be achieved by introducing said wheat Taarf25-B mutant into said plant.
In a fifth aspect, the invention provides an application of wheat TaARF25-B protein or a coding gene thereof or a biological material containing the gene in regulating and controlling plant height, grain size, grain weight or yield of plants.
Specifically, the invention provides application of wheat TaARF25-B protein or coding gene thereof or biological material containing the gene in plant height increase, grain size increase, grain weight increase or yield increase. The application can be realized by improving the expression quantity of the wheat TaARF25-B protein in the plant.
The invention also provides application of the wheat TaARF25-B protein or the coding gene thereof or the biological material containing the gene in reducing plant height, grain size, grain weight or yield. The application can be realized by reducing the expression level of the wheat TaARF25-B protein in the plant.
In a sixth aspect, the invention provides application of wheat TaARF25-B protein or an inhibitor of a coding gene thereof or a biological material containing the inhibitor in regulation and control of plant height, grain size, grain weight or yield of a plant.
The invention also provides a wheat TaARF25-B protein, a coding gene thereof, a biological material containing the gene, an inhibitor of the wheat TaARF25-B protein or the coding gene thereof, and application of the biological material containing the inhibitor in germplasm resource improvement, genetic breeding or transgenic plant construction of plant height, grain size, grain weight or yield.
Preferably, the application is to reduce the plant height, grain size, grain weight or yield of the plant by reducing the expression level of the wheat TaARF25-B protein.
In the above application, the inhibitor includes protein, DNA or RNA capable of inhibiting the expression of the wheat TaARF25-B protein.
In the present invention, the transgenic plant is preferably a transgenic plant having excellent plant height and grain traits (grain size, grain weight).
In the invention, the wheat TaARF25-B protein has any one of the following amino acid sequences:
(1) an amino acid sequence shown as SEQ ID NO. 3;
(2) the amino acid sequence of the protein with the same function is obtained by replacing, deleting or inserting one or more amino acids in the amino acid sequence shown in SEQ ID NO. 3.
The amino acid sequence shown as SEQ ID NO.3 is the amino acid sequence of the protein coded by the TaARF25-B gene on the wheat chromosome 5B, and a person skilled in the art can substitute, delete and/or add one or more amino acids according to the amino acid sequence disclosed by the invention and the conventional technical means in the field such as conservative substitution of the amino acids without influencing the activity of the amino acid sequence, so as to obtain the mutant of the TaARF25-B protein with the same activity as the TaARF25-B protein disclosed by the invention.
The nucleotide sequence of the coding gene of the TaARF25-B protein is shown as SEQ ID NO.4, and the gene is positioned on a 5B chromosome of wheat. The coding gene of the TaARF25-B protein can be any nucleotide sequence capable of coding the TaARF25-B protein. Considering the degeneracy of codons and the preference of codons of different species, the skilled person can use codons suitable for the expression of a particular species as required.
In a seventh aspect, the present invention provides a method for regulating plant height, grain size, grain weight or yield of a plant, comprising: and regulating the expression of the wheat TaARF25-B protein in the plant by a gene editing, mutagenesis, hybridization, backcrossing, selfing or asexual propagation method so as to regulate the plant height, grain size, grain weight or yield of the plant.
The method specifically comprises the following steps: reducing the plant height, grain size, grain weight or yield of the plant by reducing expression of wheat TaARF25-B protein in the plant.
Or increasing the plant height, grain size, grain weight or yield of the plant by increasing the expression of the wheat middling TaARF25-B protein in the plant.
Specifically, the method can be realized by an EMS mutagenesis technology, and through EMS mutagenesis, the encoding gene of the wheat TaARF25-B protein can generate point mutation, so that the encoded amino acid sequence can generate frame shift mutation or early termination, thereby reducing the expression of the protein. Through the identification of the mutants, the mutants with the amino acid sequences terminated in advance can be screened, and the phenotype identification is carried out, so that the mutants with excellent plant height and grain properties are obtained.
In the present invention, the plant is a monocotyledon or a dicotyledon. The plants include, but are not limited to, wheat, rice, corn, soybean, cotton, peanut, arabidopsis, and the like, preferably wheat.
The invention has the beneficial effects that:
according to the invention, a tetraploid mutant of a TaARF25-B gene is obtained by screening an EMS mutant library, and sequence analysis is carried out on the mutant to discover a wheat Taarf25-B mutant, so that the plant height, grain size and thousand seed weight of wheat can be obviously reduced by the mutant. The invention discovers that the wheat TaARF25-B gene has the function of regulating and controlling plant height, grain size and grain weight, and the plant height, the grain size (grain length and grain width) and the grain weight (thousand grain weight) can be reduced by inhibiting the expression of the wheat TaARF25-B gene. The Taarf25-B mutant, the TaARF25-B gene and the function thereof and the Taarf25-B tetraploid mutant provided by the invention have good application values, and provide important gene resources and methods for improving the seed properties and yield of wheat.
Drawings
FIG. 1 shows the results of the expression profile analysis of the wheat TaARF25-B gene in immature kernels in example 1 of the present invention, wherein 0, 2, 4, 6, 8 and 10 represent days after pollination.
FIG. 2 shows the results of mutation sites detection of two Taarf25-B mutants in example 3 of the present invention, wherein A is the result of detection of Kronos824 and B is the result of detection of Kronos 2217.
FIG. 3 is a schematic diagram of the mutation sites of two Taarf25-B mutants on TaARF25-B gene screened in example 3 of the present invention.
FIG. 4 is a schematic diagram of the amino acid structures of two Taarf25-B mutants in example 3 of the present invention.
Fig. 5 shows the expression level of TaARF25-B gene in mutant in example 4 of the present invention, wherein WT represents wild type plants, Kronos824 and Kronos2217 represent different mutant lines, n-3, p <0.05, p < 0.01.
FIG. 6 is a phenotypic picture of grain length of two Taarf25-B mutants in example 5 of the present invention, wherein WT represents wild type plant, and Kronos824 and Kronos2217 represent different mutant lines, respectively.
FIG. 7 is a phenotypic plot of grain width of Taarf25-B tetraploid mutant in example 5 of the present invention, in which WT represents the wild type plant, and Kronos824 and Kronos2217 represent different mutant lines, respectively.
Fig. 8 is the statistical results of the grain phenotype of the TaARF25-B gene of triticum aestivum of example 5, wherein a is grain length, B is grain width, C is thousand grain weight, n is 10, p is <0.05, and p is < 0.01.
FIG. 9 is a diagram showing the height phenotype of the tetraploid mutant plant of Taarf25-B in example 5 of the present invention, wherein WT represents the wild type plant, and Kronos824 and Kronos2217 represent different mutant lines.
Fig. 10 is a statistical graph of the plant height phenotype of the Taarf25-B tetraploid mutant in example 5 of the present invention, wherein WT represents wild type plants, Kronos824 and Kronos2217 represent different mutant lines, n is 10, p is <0.05, p is < 0.01.
Detailed Description
The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Example 1 expression profiling of the TaARF25 Gene
The expression profile of the TaARF25 gene in the early grain development stage is detected by real-time quantitative fluorescent PCR (quantitative real time RT-PCR), and PCR amplification is carried out by using primer sequences shown as SEQ ID No.7 and SEQ ID No. 8. The results are shown in FIG. 1. The result shows that the TaARF25 gene is mainly dominantly expressed in grains after pollination for 0 day. Based on this, tetraploid mutants of Taarf25-B were further obtained by screening tetraploid pools.
Example 2 creation of Taarf25-B mutant plants
In the embodiment, the expression level of TaARF25-B is inhibited in wheat by using EMS mutagenesis principle, and the specific method is as follows:
(1) treating wheat seeds by using an EMS mutagen, which specifically comprises the following steps: selecting wheat seeds with uniform size, washing dust on the surfaces of the seeds with sterile water, soaking the seeds in clear water for 2 hours, and then carrying out mutagenesis treatment; EMS solution with the mass concentration of 0.5-1.5% is adopted to soak the seeds for 6-12 h. After soaking, washing the seeds with sterile water, and sucking the water on the surfaces of the seeds.
(2) Planting the treated seeds in a nutrition pot: sowing the mutagenized seeds (marked as T1) in a nutrition pot (8cm multiplied by 8cm) filled with nutrient soil, covering films to ensure that the seeds subjected to EMS mutagenesis can normally germinate, and removing the covered films after 3-5 days.
(3) Transplanting seedlings: as the EMS treatment can damage genome DNA and cause that the germination time of partial seeds is prolonged to form weak seedlings, after the seeds germinate and grow for two weeks in the step (2), spraying a monopotassium phosphate aqueous solution with the mass concentration of 0.05 percent to ensure that the seedlings normally grow so as to improve the EMS mutagenesis efficiency. Transplanting the seedlings to a greenhouse after 3-4 weeks, and performing conventional management.
(4) And identifying the mutants to ensure that the mutants are positive plants, respectively harvesting the positive plants according to single plants, continuously planting the positive plants until mutation sites are homozygous, and carrying out phenotype statistics on the homozygous positive plants.
Example 3 detection of Taarf25-B mutant Positive plants
Designing a primer according to a reference sequence of TaARF25-B gene genome DNA, taking leaves of a mutant plant in a three-leaf period for genome DNA extraction, and carrying out PCR amplification by using a primer sequence shown as SEQ ID NO.9-SEQ ID NO.12, wherein the PCR program is as follows: 5 minutes at 94 ℃; 30 seconds at 94 ℃; at 56 ℃ for 30 seconds; 30 seconds at 72 ℃; repeating for 34 times; 72 ℃ for 10 minutes.
The PCR system was as follows:
and (3) carrying out electrophoresis detection, and sending the PCR product to a biological engineering (Shanghai) corporation for sequencing so as to determine the mutation type of the plant. Wherein, the mutant plant Kronos824 has early termination of amino acid due to mutation of C-T at 3914 th position of TaARF25-B gene, the mutant plant Kronos2217 has early termination of encoded protein due to mutation of G-A at 2614 th position of TaARF25-B gene, the detection result is shown in figure 2, the position of the mutation site on TaARF25-B gene is shown in figure 3, and the amino acid structure of the mutant protein is shown in figure 4.
Example 4 detection of expression level of TaARF25-B Gene in EMS mutagenesis Strain
The expression level of TaARF25-B gene in the EMS mutant strain constructed in example 2 and example 3 was detected by real-time quantitative fluorescent PCR (RT-PCR), and PCR amplification was performed using the primer sequences shown in SEQ ID NO.13 and SEQ ID NO. 14. The results are shown in FIG. 5, which shows that the expression level of TaARF25-B is significantly reduced in Kronos824 and Kronos2217, indicating that the expression of TaARF25-B gene can be suppressed by EMS mutagenesis technique.
Example 5 Effect of wheat TaARF25-B Gene on plant height and grain development
EMS mutant plants Kronos824 and Kronos2217 were incubated simultaneously with their wild type control plants in the greenhouse (16 h light, 20 ℃/8 h dark, 22 ℃ photoperiod) until the entire life cycle was completed. Phenotype observation and statistics are carried out on the plant height and mature grains of the EMS mutant plants of the T4 generation, the grain length and grain width phenotypes are shown in figures 6 and 7, and the statistical results of the grain length, grain width and thousand kernel weight phenotypes of the EMS mutant plants are shown in figure 8. The phenotype of the plant height of the EMS mutant plant is shown in figure 9, the statistical result is shown in figure 10, and the result shows that compared with the wild type, the plant height of a mutant plant line is reduced, the grain length, the grain width and the thousand grain weight are all obviously reduced, and the reduction of the grain weight is the result of the combined action of the grain length and the grain width. The results show that the wheat TaARF25-B gene has the function of regulating plant height and grain size and can influence grain length, grain width and thousand kernel weight.
Although the invention has been described in detail with respect to the general description and the specific embodiments thereof, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Accordingly, it is intended that all such modifications and alterations be included within the scope of this invention as defined in the appended claims.
Sequence listing
<110> research center of Dongying salt-tolerant crops in the institute of crop science of Chinese academy of agricultural sciences
<120> wheat TaARF25-B gene, mutant thereof and application thereof in regulating plant height and grain traits
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Ser Gln Ser Ser Phe Tyr Gln Gln Asn Leu Leu Glu Gly Asn Asn Asp
580 585 590
Pro Ser Leu His Leu His Asn Gly Phe Arg Asn Phe Ser Ser Gln Asp
595 600 605
Ser Ser Asn Leu Val Ser Leu Pro Arg Thr Asp Gln Leu Met Ala Pro
610 615 620
Glu Gly Trp Pro Ser Lys Arg Leu Ala Val Glu Pro Leu Gly His Ile
625 630 635 640
Glu Ser Arg Ser Met Gln Pro Lys His Glu Asn Ile Asn His Gln Ser
645 650 655
Asn Met Ser His Phe Ala Gly Thr Leu Ala Pro Gln Ser Ala Arg Asp
660 665 670
Ser Ser Ser Val Gln Ala Tyr Gly Ala Asn Ala Asp Asn Gln Phe Leu
675 680 685
Ser Ser Thr Phe Ala Phe Gln Asp Gly Met Ala Gly Ala Arg Gly Gly
690 695 700
Ser Ser Ser Gly Thr Val Ser Met Ala Ile Pro Leu Leu Arg Tyr Ser
705 710 715 720
Gly Glu Asp Leu Pro Pro Ala Asp Thr Leu Ala Thr Ser Ser Cys Leu
725 730 735
Gly Glu Ser Gly Thr Phe Asn Ser Leu Asp Asn Met Cys Gly Val Asn
740 745 750
Pro Ser Gln Asp Gly Thr Phe Val Lys Val Tyr Lys Ser Gly Ser Pro
755 760 765
Gly Arg Ser Leu Asp Ile Thr Lys Phe Ser Ser Tyr Tyr Glu Leu Arg
770 775 780
Ser Glu Leu Glu His Leu Phe Gly Leu Glu Gly Gln Leu Glu Asp Pro
785 790 795 800
Val Arg Ser Gly Trp Gln Leu Val Phe Val Asp Arg Glu Asn Asp Ile
805 810 815
Leu Leu Val Gly Asp Asp Pro Trp Gln Glu Phe Val Asn Ser Val Gly
820 825 830
Cys Ile Lys Ile Leu Ser Gln Gln Glu Val Gln Gln Met Val Arg Gly
835 840 845
Gly Glu Gly Leu Val Ser Ser Ala Pro Gly Ala Arg Met Ala Gln Gly
850 855 860
Asn Val Cys Asp Gly Tyr Ser Gly Gly His Asp Leu Gln Asn Leu Thr
865 870 875 880
Gly Asn Met Ala Ser Val Pro Pro Leu Asp Tyr
885 890
<210> 4
<211> 2676
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
atggagagaa agctctccat gtccgagatg ccgcagtccc tcccggaaaa cgatggggaa 60
caaaggtgtc tgaactcgga gctatggcat gcatgcgctg ggccccttgt gtctctgccc 120
gcggttggaa gccgggtcat ctattttcca cagggccata gtgagcaggt tgccgcatca 180
actaataagg aggttgatgc tcaaatccct aattatccaa atctcccccc tcagttgatc 240
tgccagcttc ataatgtcac catgcatgct gatgcagaga ctgatgaagt ttatgcccaa 300
atgacactgc agcccttgag cccggaagag caaaaggagc cttttcttcc aatcgagtta 360
ggtgctgcta gcaagcagcc gactaattac ttctgcaaga ctttgactgc aagtgataca 420
agtacacatg gcggattttc tgttcctcgc cgttctgctg agaaagtctt ccctccattg 480
gatttttctc tgcagcctcc atgccaggag ctcattgcaa aagatctgca tgataatgaa 540
tggaaatttc gccacatatt ccgtggtcag ccgaaaaggc atctcctgac tacaggttgg 600
agtgtctttg taagtgcaaa gcgactagta gcaggggatt ctgttatttt catctggaat 660
gataataacc aacttctctt ggggattcgt catgcaaatc gtcctcagac aattatgccg 720
tcttctgtgc tgtcaagcga cagcatgcat ataggtcttc ttgcagcagc agctcatgct 780
gcggccacaa acagccgttt cactattttc tataaccccc gggctagccc ttctgagttc 840
atcatcccac tggctaagta tgtcaaatct gtttaccaca cacgtgtgtc tgttggaatg 900
cggtttagaa tgctttttga gacagaggag tcaagtgtca gacgatacat gggtacaatc 960
acaaccataa gtgatcttga ctctgtgcgt tggccaaatt cacattggcg ttctgtgaag 1020
gttggttggg acgagtctac cgctggtgag aaacaaccta gagtgtcact ctgggagatt 1080
gagccattga caacctttcc tatgtatcca actgcttttc ctttaaggct gaagcgtcca 1140
tgggcttcag ggctgccttc tatgcatggc atgttcaatg gtgtcaagaa tgatgatttt 1200
gctcgttatt cttctctcat gtggctcgga gatggggata gaggggctca gtcgttgaac 1260
ttccagggag ttggagcgtc accttggctt cagccaagaa tagattctcc gttgctgggt 1320
cttaagccag acacatacca gcaaatggct gcagcagcac tggaagaaat taggaccggg 1380
gatccttcga aacagtcctc agctctcttg caattccaac agactcagaa tccgaacggt 1440
gggttgaatt ctgtgtatgc caatcatgtt ctgcagcaga tgcagtacca ggctcagcag 1500
tcgtctctgc agactgttca gcacggccat agtcagtaca gtggtaatcc tgggtttctt 1560
caaagccagt ttcagcagct gcatttgcat aatcctccgg caccgccgca gcaagggtat 1620
caggttatac aacaatctca ccaggagatg caacagcagc tttcatctgg ttgtcgtcgt 1680
atttccgatg tagattctag catgcctggt tctgagtctg cttcgcagtc acaatcttca 1740
ttctaccagc agaatctctt agaaggaaac aatgatccat cgttgcatct gcacaatggt 1800
tttcgtaact tctctagcca agattcctca aaccttgtta gtttgcctcg aactgaccaa 1860
ttaatggcgc cggagggatg gccttcaaag aggttggctg tggaacccct tggtcatatt 1920
gaatctcggt ctatgcaacc caaacatgag aacataaatc accagagtaa tatgtcccac 1980
tttgctggca ccttggcgcc acagtcagca agagactcat ccagtgtcca ggcttatggt 2040
gcaaatgctg acaaccagtt tctgtcatca acatttgcat tccaggacgg aatggcaggt 2100
gcaaggggtg gcagcagcag cggaactgtt tctatggcca tacctttgtt gaggtatagt 2160
ggcgaagatt tgccacccgc ggacacctta gcaacttcca gttgtttagg cgaatcggga 2220
accttcaact ctcttgataa tatgtgtggt gtaaacccat cacaagatgg aacctttgtg 2280
aaggtttaca aatcagggtc ccctggaaga tcgctcgaca tcaccaaatt cagcagctac 2340
tacgagttac gtagtgagct ggagcatcta tttggcctcg aaggccaact ggaggacccc 2400
gtaagatcag gctggcagct tgtattcgtc gaccgagaaa atgatattct tctcgtcggc 2460
gacgacccat ggcaggagtt tgtgaatagc gtagggtgca tcaagatact ctcgcagcag 2520
gaggtgcagc agatggtccg cggcggcgag ggccttgtgt cttctgcacc tggagcgagg 2580
atggcgcagg gcaacgtctg cgacggttat tctgggggcc acgacctgca gaatctcacc 2640
ggcaacatgg cctccgtacc gccgctggac tactga 2676
<210> 5
<211> 2058
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
atggagagaa agctctccat gtccgagatg ccgcagtccc tcccggaaaa cgatggggaa 60
caaaggtgtc tgaactcgga gctatggcat gcatgcgctg ggccccttgt gtctctgccc 120
gcggttggaa gccgggtcat ctattttcca cagggccata gtgagcaggt tgccgcatca 180
actaataagg aggttgatgc tcaaatccct aattatccaa atctcccccc tcagttgatc 240
tgccagcttc ataatgtcac catgcatgct gatgcagaga ctgatgaagt ttatgcccaa 300
atgacactgc agcccttgag cccggaagag caaaaggagc cttttcttcc aatcgagtta 360
ggtgctgcta gcaagcagcc gactaattac ttctgcaaga ctttgactgc aagtgataca 420
agtacacatg gcggattttc tgttcctcgc cgttctgctg agaaagtctt ccctccattg 480
gatttttctc tgcagcctcc atgccaggag ctcattgcaa aagatctgca tgataatgaa 540
tggaaatttc gccacatatt ccgtggtcag ccgaaaaggc atctcctgac tacaggttgg 600
agtgtctttg taagtgcaaa gcgactagta gcaggggatt ctgttatttt catctggaat 660
gataataacc aacttctctt ggggattcgt catgcaaatc gtcctcagac aattatgccg 720
tcttctgtgc tgtcaagcga cagcatgcat ataggtcttc ttgcagcagc agctcatgct 780
gcggccacaa acagccgttt cactattttc tataaccccc gggctagccc ttctgagttc 840
atcatcccac tggctaagta tgtcaaatct gtttaccaca cacgtgtgtc tgttggaatg 900
cggtttagaa tgctttttga gacagaggag tcaagtgtca gacgatacat gggtacaatc 960
acaaccataa gtgatcttga ctctgtgcgt tggccaaatt cacattggcg ttctgtgaag 1020
gttggttggg acgagtctac cgctggtgag aaacaaccta gagtgtcact ctgggagatt 1080
gagccattga caacctttcc tatgtatcca actgcttttc ctttaaggct gaagcgtcca 1140
tgggcttcag ggctgccttc tatgcatggc atgttcaatg gtgtcaagaa tgatgatttt 1200
gctcgttatt cttctctcat gtggctcgga gatggggata gaggggctca gtcgttgaac 1260
ttccagggag ttggagcgtc accttggctt cagccaagaa tagattctcc gttgctgggt 1320
cttaagccag acacatacca gcaaatggct gcagcagcac tggaagaaat taggaccggg 1380
gatccttcga aacagtcctc agctctcttg caattccaac agactcagaa tccgaacggt 1440
gggttgaatt ctgtgtatgc caatcatgtt ctgcagcaga tgcagtacca ggctcagcag 1500
tcgtctctgc agactgttca gcacggccat agtcagtaca gtggtaatcc tgggtttctt 1560
caaagccagt ttcagcagct gcatttgcat aatcctccgg caccgccgca gcaagggtat 1620
caggttatac aacaatctca ccaggagatg caacagcagc tttcatctgg ttgtcgtcgt 1680
atttccgatg tagattctag catgcctggt tctgagtctg cttcgcagtc acaatcttca 1740
ttctaccagc agaatctctt agaaggaaac aatgatccat cgttgcatct gcacaatggt 1800
tttcgtaact tctctagcca agattcctca aaccttgtta gtttgcctcg aactgaccaa 1860
ttaatggcgc cggagggatg gccttcaaag aggttggctg tggaacccct tggtcatatt 1920
gaatctcggt ctatgcaacc caaacatgag aacataaatc accagagtaa tatgtcccac 1980
tttgctggca ccttggcgcc acagtcagca agagactcat ccagtgtcca ggcttatggt 2040
gcaaatgctg acaactag 2058
<210> 6
<211> 1008
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 6
atggagagaa agctctccat gtccgagatg ccgcagtccc tcccggaaaa cgatggggaa 60
caaaggtgtc tgaactcgga gctatggcat gcatgcgctg ggccccttgt gtctctgccc 120
gcggttggaa gccgggtcat ctattttcca cagggccata gtgagcaggt tgccgcatca 180
actaataagg aggttgatgc tcaaatccct aattatccaa atctcccccc tcagttgatc 240
tgccagcttc ataatgtcac catgcatgct gatgcagaga ctgatgaagt ttatgcccaa 300
atgacactgc agcccttgag cccggaagag caaaaggagc cttttcttcc aatcgagtta 360
ggtgctgcta gcaagcagcc gactaattac ttctgcaaga ctttgactgc aagtgataca 420
agtacacatg gcggattttc tgttcctcgc cgttctgctg agaaagtctt ccctccattg 480
gatttttctc tgcagcctcc atgccaggag ctcattgcaa aagatctgca tgataatgaa 540
tggaaatttc gccacatatt ccgtggtcag ccgaaaaggc atctcctgac tacaggttgg 600
agtgtctttg taagtgcaaa gcgactagta gcaggggatt ctgttatttt catctggaat 660
gataataacc aacttctctt ggggattcgt catgcaaatc gtcctcagac aattatgccg 720
tcttctgtgc tgtcaagcga cagcatgcat ataggtcttc ttgcagcagc agctcatgct 780
gcggccacaa acagccgttt cactattttc tataaccccc gggctagccc ttctgagttc 840
atcatcccac tggctaagta tgtcaaatct gtttaccaca cacgtgtgtc tgttggaatg 900
cggtttagaa tgctttttga gacagaggag tcaagtgtca gacgatacat gggtacaatc 960
acaaccataa gtgatcttga ctctgtgcgt tggccaaatt cacattga 1008
<210> 7
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
gaatgctttt tgagacagag ga 22
<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
aatggctcaa tctcccagag t 21
<210> 9
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
atgcaaccca aacatgagaa ca 22
<210> 10
<211> 25
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
atcttcgcca ctatacctca acaaa 25
<210> 11
<211> 25
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 11
gctctatttc tatgtctaat taaca 25
<210> 12
<211> 25
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 12
cagagttaat aggttgagga ataac 25
<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 13
agccattgac aacctttcct a 21
<210> 14
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 14
cccagcaacg gagaatcta 19
Claims (4)
1. The wheat Taarf25-B mutant or the coding gene thereof or the biological material containing the coding gene is applied to reducing the plant height, the grain size, the grain weight or the yield of wheat,
the amino acid sequence of the wheat Taarf25-B mutant is shown in SEQ ID NO.1 or SEQ ID NO. 2;
the biological material is an expression cassette, a vector or a host cell.
2. The application of the wheat TaARF25-B protein or the coding gene thereof or the biomaterial containing the gene in regulating and controlling the plant height, the grain size, the grain weight or the yield of wheat,
the amino acid sequence of the wheat TaARF25-B protein is shown in SEQ ID NO. 3;
the biological material is an expression cassette, a vector or a host cell.
3. The application of the wheat TaARF25-B protein or the coding gene thereof or the biological material containing the gene in the germplasm resource improvement of wheat plant height, grain size, grain weight or yield,
the amino acid sequence of the wheat TaARF25-B protein is shown in SEQ ID NO. 3;
the biological material is an expression cassette, a vector or a host cell.
4. A method for reducing the plant height, grain size, grain weight or yield of wheat is characterized in that a coding gene of TaARF25-B protein in wheat is mutated into a gene of a wheat Taarf25-B mutant with a coding sequence shown as SEQ ID NO.1 or SEQ ID NO.2, so that the plant height, grain size, grain weight or yield of wheat is reduced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110540174.7A CN113248585B (en) | 2021-05-18 | 2021-05-18 | Wheat TaARF25-B gene, mutant thereof and application thereof in regulating plant height and grain traits |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110540174.7A CN113248585B (en) | 2021-05-18 | 2021-05-18 | Wheat TaARF25-B gene, mutant thereof and application thereof in regulating plant height and grain traits |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113248585A CN113248585A (en) | 2021-08-13 |
| CN113248585B true CN113248585B (en) | 2022-07-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| Country | Link |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110724705A (en) * | 2019-11-19 | 2020-01-24 | 中国农业科学院作物科学研究所 | Application of wheat TaIAA21 gene in regulation and control of seed traits |
| CN110846323A (en) * | 2019-11-07 | 2020-02-28 | 中国农业科学院作物科学研究所 | Wheat TaARF12 gene and application thereof |
-
2021
- 2021-05-18 CN CN202110540174.7A patent/CN113248585B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110846323A (en) * | 2019-11-07 | 2020-02-28 | 中国农业科学院作物科学研究所 | Wheat TaARF12 gene and application thereof |
| CN110724705A (en) * | 2019-11-19 | 2020-01-24 | 中国农业科学院作物科学研究所 | Application of wheat TaIAA21 gene in regulation and control of seed traits |
Non-Patent Citations (12)
| Title |
|---|
| A review of auxin response factors (ARFs) in Plants;Si-Bei Li等;《frontiers in Plant Science》;20160203;第7卷;1-7 * |
| ARF-Aux/IAA interactions through domain III/IV are not strictly required for auxin-responsive gene expression;Shucai Wang等;《Plant Signaling & Behavior》;20130630;第8卷(第6期);e24526 * |
| hypotherical protein CFC21_069863;Zimin A.V.等;《Genbank登录号：KAF7063335》;20200820;1-2 * |
| hypotherical protein CFC21_069875;Zimin A.V.等;《Genbank登录号：KAF7063349.1》;20200820;1-2 * |
| Irrepressible，truncated auxin response factors;Ckurshumova W.等;《Plant Signaling & Behavior》;20120831;第7卷(第8期);1027-1030 * |
| None.UniProtKB-A0A3B6LGA4(A0A3B6LGA4_WHEAT).《UniProtKB-A0A3B6LGA4》.2021, * |
| UniProtKB-A0A3B6LGA4(A0A3B6LGA4_WHEAT);None;《UniProtKB-A0A3B6LGA4》;20210407;1-6 * |
| Zimin A.V.等.hypotherical protein CFC21_069863.《Genbank登录号：KAF7063335》.2020, * |
| Zimin A.V.等.hypotherical protein CFC21_069875.《Genbank登录号：KAF7063349.1》.2020, * |
| 小麦ARF基因家族生物信息学分析及在干旱胁迫下的表达特性研究;孙仁玮 等;《植物遗传资源学报》;20170911;第19卷(第1期);122-134 * |
| 植物生长素原初响应基因Aux/IAA研究进展;司马晓娇 等;《浙江农林大学学报》;20150420;第32卷(第2期);313-318 * |
| 生长素响应因子ARF研究进展;关晓溪 等;《分子植物育种》;20160728;第14卷(第7期);1892-1897 * |
Also Published As
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|---|---|
| CN113248585A (en) | 2021-08-13 |
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