CN113233923A - Process for preparing polypeptide organic fertilizer from blood of slaughtered livestock - Google Patents
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- CN113233923A CN113233923A CN202110501239.7A CN202110501239A CN113233923A CN 113233923 A CN113233923 A CN 113233923A CN 202110501239 A CN202110501239 A CN 202110501239A CN 113233923 A CN113233923 A CN 113233923A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/10—Addition or removal of substances other than water or air to or from the material during the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/40—Treatment of liquids or slurries
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a process for preparing a polypeptide organic fertilizer by using slaughtered livestock blood, which relates to the technical field of deep processing of livestock blood and solves the technical problems of complex process, long process time, high process energy consumption, high process cost and difficult preservation of the produced organic fertilizer in the conventional process method for preparing the organic fertilizer by using animal blood.
Description
Technical Field
The invention relates to the technical field of deep processing of livestock blood, in particular to a process for preparing a polypeptide organic fertilizer by using slaughtered livestock blood.
Background
The blood of the livestock contains various nutrient components such as protein, amino acid, vitamin, carbohydrate, lipid, mineral elements and the like, wherein the content of the fresh blood protein is up to 18-22 percent, and the fresh blood protein is the largest animal protein resource. At present, the utilization of livestock blood in China is mainly used for producing protein feed, biological agents, additives, industrial raw materials and direct eating.
According to statistics, only 25% of blood of slaughtered livestock is centrally and reasonably utilized every year, and most of blood is discharged as waste, so that the environment is seriously polluted. Therefore, how to reasonably utilize blood of slaughtered livestock and poultry, improve the centralized utilization rate of the blood of the livestock and poultry, and reduce resource waste and environmental pollution is very important.
Chinese patent CN110642649A discloses a process for preparing organic fertilizer by using animal blood, which uses the animal blood which originally needs to spend a large amount of capital treatment in slaughter houses as raw material, and the raw material is processed by biological treatment such as multi-stage protein hydrolysis, emulsification, enzymolysis, fermentation and the like to become microbial organic fertilizer to be returned to the field, the process flow of multi-stage protein hydrolysis, emulsification, enzymolysis, fermentation, repeated concentration and the like in the invention is complex, the time cost of the process flow is about 37 h-56 h, the preparation time and the concentration time are not counted, the time is long, and most of the production time in 37-56 h requires constant temperature of 40-55 ℃, and the temperature is required to be 115-121 ℃ during high temperature sterilization for 30min, a large amount of energy is consumed during the production according to the process, the processing cost and the production difficulty of the product are increased, and the finally obtained organic fertilizer requires low temperature preservation, the transportation and storage costs are easily increased, which is not favorable for practical application.
Therefore, the invention provides a process for preparing a polypeptide organic fertilizer by using blood of slaughtered livestock and poultry aiming at the problems.
Disclosure of Invention
The invention aims to: the invention provides a process for preparing a polypeptide organic fertilizer by using blood of slaughtered livestock, aiming at solving the problems of complex process, long process time, high process energy consumption, high process cost and difficult preservation of the produced organic fertilizer in the existing process method for preparing the organic fertilizer by using animal blood.
The invention specifically adopts the following technical scheme for realizing the purpose:
a process for preparing a polypeptide organic fertilizer by using blood of slaughtered livestock comprises the following steps:
step one, preparing blood cell liquid: collecting blood of fresh slaughtered livestock in a slaughterhouse, adding trisodium citrate with the mass percentage of 0.75-0.85%, fully stirring for 5-10 min, then carrying out centrifugal separation, removing supernatant, and collecting red blood cells to obtain blood cell liquid for later use;
step two, cell membrane breaking, merging and enzymolysis: adding deionized water with the mass 1 time of that of the blood cell liquid obtained in the step one into the blood cell liquid, stirring the mixture for 3 to 5 minutes, adjusting the pH value of the mixed solution to 7.5 to 10.0, then adding endo-protease, heating the mixture to 45 to 60 ℃, continuously stirring the mixture at the temperature to carry out preliminary enzymolysis for 25 to 35 minutes, and continuing the enzymolysis for 3.5 to 4.0 hours to obtain enzymolysis liquid;
step three, preparing a polypeptide organic fertilizer: and adjusting the pH value of the enzymolysis liquid obtained in the step two to 5.8-6.0, inactivating the enzyme, adding potassium sorbate, cooling to the ambient temperature by using water circulation, and performing fixed-solution treatment to obtain the active polypeptide fertilizer.
Furthermore, in the centrifugal separation process, the centrifugal rate is 2600r/min to 3400r/min, and the centrifugal time is 10min to 20 min.
Further, the amount of endoprotease added E/S was 8000U/g.
Further, starting ultrasonic waves for carrying out ultrasonic hemolysis treatment while carrying out preliminary enzymolysis for 25-35 min, wherein the frequency of the ultrasonic waves is 26-30 Khz, and the interval between ultrasonic time and intermittent time is 1: 1. the ultrasonic cavitation can accelerate the rupture speed of erythrocyte membranes, and the ultrasonic wave can averagely improve the enzyme activity by 21.18 percent.
Further, adding a nonionic surfactant into the ultrasonic hemolysis treatment, wherein the ratio of the nonionic surfactant in the mixed solution is 0.2-1.0%, and the mass ratio of the nonionic surfactant is 1: 1 and polyoxyethylene. Has stronger dissolving effect on cell membranes, can effectively promote enzyme activity, increases the hemolysis efficiency by adding the nonionic surfactant, and simultaneously improves the enzyme activity.
Further, the ratio of potassium sorbate in the enzymatic hydrolysate was 0.1%.
The invention has the following beneficial effects:
the invention relates to a process for preparing a polypeptide organic fertilizer by using blood of slaughtered livestock, which utilizes a modern biological and biochemical integrated technology to prepare a process for preparing a polypeptide fertilizer by using the blood of the slaughtered livestock, carries out enzymolysis on protein in the blood of the slaughtered livestock into micromolecular polypeptide, furthest reserves other vitamins, saccharides, mineral elements and the like which are beneficial to the growth of soil and crops, carries out enzymolysis on the protein in the blood into polypeptide and is applied to agricultural fertilizer products, supplements organic matters and trace elements for the soil and plants, realizes high-valued development of the blood of the livestock, has no pollution, and is suitable for large-scale industrial production;
in the process for preparing the polypeptide organic fertilizer by using the blood of slaughtered livestock, the finished product can be obtained by collecting and separating the blood of the livestock, breaking the cell membrane and carrying out enzymolysis, and the process is simple;
in the process for preparing the polypeptide organic fertilizer by using blood of slaughtered livestock and poultry, as the ultrasonic wave and nonionic surfactant combined membrane rupture process is adopted, the enzymolysis time can be shortened from 6 to 8 hours to 4 hours, the enzyme is directly inactivated by reducing the pH value without high-temperature inactivation, the enzyme activity can be effectively inhibited, and in addition, the treatment such as biological microbial inoculum fermentation is not needed, the polypeptide biological fertilizer with higher activity can be obtained, so that the energy consumption is saved, the cost performance is greatly reduced, the production efficiency is improved, and the process has the advantages of time saving, energy saving and high efficiency;
the polypeptide organic fertilizer prepared from the blood of slaughtered livestock is easy to store and only needs to be stored at normal temperature, and is suitable for industrial large-scale production and convenient to use.
Drawings
FIG. 1 is a schematic diagram of the molecular weight distribution of the polypeptide organic fertilizer prepared in example 1 of the present invention;
FIG. 2 is a schematic diagram of the molecular weight distribution of the polypeptide organic fertilizer prepared in example 2 of the present invention;
FIG. 3 is a schematic diagram of the molecular weight distribution of the polypeptide organic fertilizer prepared in example 3 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention are clearly and completely described below,
example 1
Process for preparing polypeptide organic fertilizer from blood of slaughtered livestock
The method comprises the following steps:
step one, preparing blood cell liquid: collecting blood of fresh slaughtered livestock in slaughterhouse, adding trisodium citrate 0.85 wt%, stirring for 10min, centrifuging, removing supernatant, and collecting red blood cells to obtain blood cell liquid; wherein the centrifugation rate is 3400r/min, and the centrifugation time is 20 min;
step two, cell membrane breaking, merging and enzymolysis: adding deionized water with the mass 1 time of that of the blood cell liquid obtained in the step one into the blood cell liquid, stirring the mixture for 5 minutes, then adjusting the pH value of the mixed solution to 10.0, then adding endo-protease, heating the mixture to 60 ℃, continuously stirring the mixture at the temperature to carry out preliminary enzymolysis for 35 minutes, and then continuing the enzymolysis for 4.0 hours to obtain an enzymolysis liquid; wherein the addition amount of endoprotease E/S is 8000U/g;
and starting ultrasonic waves to perform ultrasonic hemolysis treatment while performing preliminary enzymolysis for 35min, wherein the ultrasonic frequency is 30Khz, and the ultrasonic time and the interval time are 1: 1; and adding a nonionic surfactant while performing ultrasonic hemolysis treatment, wherein the proportion of the nonionic surfactant in the mixed solution is 1.0%, and the mass ratio of the nonionic surfactant is 1: 1 and polyoxyethylene.
Step three, preparing a polypeptide organic fertilizer: adjusting the pH value of the enzymolysis liquid obtained in the step two to 6.0, adding potassium sorbate, cooling to the ambient temperature by using water circulation, and performing fixed-solution treatment to obtain the active polypeptide fertilizer; wherein the proportion of potassium sorbate in the enzymolysis liquid is 0.1%.
The polypeptide organic fertilizer prepared in the above embodiment is detected, and an average relative molecular weight distribution diagram 1 of livestock blood enzymolysis is drawn, from fig. 1, it can be known that the active oligopeptide proportion with the molecular weight of 196-1000 reaches 82.98%, and the average relative molecular weight of 661 is calculated.
Example 2
Process for preparing polypeptide organic fertilizer from blood of slaughtered livestock
The method comprises the following steps:
step one, preparing blood cell liquid: collecting blood of fresh slaughtered livestock in slaughterhouse, adding trisodium citrate 0.75 wt%, stirring for 5min, centrifuging, removing supernatant, and collecting red blood cells to obtain blood cell liquid; wherein, the centrifugation rate is 2600r/min, and the centrifugation time is 10 min;
step two, cell membrane breaking, merging and enzymolysis: adding deionized water with the mass 1 time of that of the blood cell liquid obtained in the step one into the blood cell liquid, stirring the mixture for 3min, then adjusting the pH value of the mixed solution to 7.5, then adding endo-protease, heating the mixture to 45 ℃, continuously stirring the mixture at the temperature to carry out preliminary enzymolysis for 25min, and continuing enzymolysis for 3.5 hours to obtain an enzymolysis liquid; wherein the addition amount of endoprotease E/S is 8000U/g;
in the above, the ultrasonic wave is started for ultrasonic hemolysis treatment while performing preliminary enzymolysis for 25min, wherein the ultrasonic frequency is 26Khz, and the ultrasonic time and the intermittent time interval are 1: 1; and adding a nonionic surfactant while performing ultrasonic hemolysis treatment, wherein the proportion of the nonionic surfactant in the mixed solution is 1.0%, and the mass ratio of the nonionic surfactant is 1: 1 and polyoxyethylene.
Step three, preparing a polypeptide organic fertilizer: adjusting the pH value of the enzymolysis liquid obtained in the step two to 5.8, adding potassium sorbate, cooling to the ambient temperature by using water circulation, and performing fixed-solution treatment to obtain the active polypeptide fertilizer; wherein the proportion of potassium sorbate in the enzymolysis liquid is 0.1%.
The polypeptide organic fertilizer prepared in the above embodiment is detected, and an average relative molecular weight distribution graph 2 of livestock blood enzymolysis is drawn, from fig. 2, it can be known that the active oligopeptide proportion with the molecular weight of 196-1000 reaches 82.34%, and the average relative molecular weight of the active polypeptide fertilizer is 669.
Example 3
Process for preparing polypeptide organic fertilizer from blood of slaughtered livestock
The method comprises the following steps:
step one, preparing blood cell liquid: collecting blood of fresh slaughtered livestock in slaughterhouse, adding trisodium citrate 0.80 wt%, stirring for 8min, centrifuging, removing supernatant, and collecting red blood cells to obtain blood cell liquid; wherein, the centrifugation speed is 3000r/min, and the centrifugation time is 15 min;
step two, cell membrane breaking, merging and enzymolysis: adding deionized water with the mass 1 time of that of the blood cell liquid obtained in the step one into the blood cell liquid, stirring for 4min, adjusting the pH value of the mixed solution to 9.0, then adding endo-protease, heating to 55 ℃, continuously stirring at the temperature for preliminary enzymolysis for 30min, and continuing enzymolysis for 4.0 hours to obtain an enzymolysis liquid; wherein the addition amount of endoprotease E/S is 8000U/g;
step three, preparing a polypeptide organic fertilizer: and (4) adjusting the pH value of the enzymolysis liquid obtained in the step two to 5.8-6.0, adding potassium sorbate, cooling to the ambient temperature by using water circulation, and performing fixed-solution treatment to obtain the active polypeptide fertilizer.
In the above, the ultrasonic wave is started for ultrasonic hemolysis treatment while performing the preliminary enzymolysis for 30min, wherein the ultrasonic frequency is 28Khz, and the ultrasonic time and the intermittent time interval are 1: 1; and adding a nonionic surfactant while performing ultrasonic hemolysis treatment, wherein the proportion of the nonionic surfactant in the mixed solution is 0.6%, and the mass ratio of the nonionic surfactant is 1: 1 and polyoxyethylene; wherein the proportion of potassium sorbate in the enzymolysis liquid is 0.1%.
The polypeptide organic fertilizer prepared in the above embodiment is detected, and an average relative molecular weight distribution graph 3 of livestock blood enzymolysis is drawn, from fig. 3, it can be known that the active oligopeptide proportion with the molecular weight of 196-1000 reaches 82.67%, and the average relative molecular weight of the active polypeptide fertilizer is calculated to be 662.
According to the embodiment, the technical process for hydrolyzing the hemoglobin by adopting the method of combining enzymolysis, ultrasound and surfactant in the process for preparing the polypeptide organic fertilizer from the blood of slaughtered livestock and poultry greatly shortens the process time, improves the enzymolysis efficiency and reduces the production difficulty and the production cost.
Claims (6)
1. A process for preparing a polypeptide organic fertilizer by using blood of slaughtered livestock and poultry is characterized by comprising the following steps:
step one, preparing blood cell liquid: collecting blood of fresh slaughtered livestock in a slaughterhouse, adding trisodium citrate with the mass percentage of 0.75-0.85%, fully stirring for 5-10 min, then carrying out centrifugal separation, removing supernatant, and collecting red blood cells to obtain blood cell liquid for later use;
step two, cell membrane breaking, merging and enzymolysis: adding deionized water with the mass 1 time of that of the blood cell liquid obtained in the step one into the blood cell liquid, stirring the mixture for 3 to 5 minutes, adjusting the pH value of the mixed solution to 7.5 to 10.0, then adding endo-protease, heating the mixture to 45 to 60 ℃, continuously stirring the mixture at the temperature to carry out preliminary enzymolysis for 25 to 35 minutes, and continuing the enzymolysis for 3.5 to 4.0 hours to obtain enzymolysis liquid;
step three, preparing a polypeptide organic fertilizer: and (4) adjusting the pH value of the enzymolysis liquid obtained in the step two to 5.8-6.0, adding potassium sorbate, cooling to the ambient temperature by using water circulation, and performing fixed-solution treatment to obtain the active polypeptide fertilizer.
2. The process for preparing the polypeptide organic fertilizer by using the blood of slaughtered livestock and poultry according to claim 1, wherein in the first step, the centrifugal rate is 2600-3400 r/min and the centrifugal time is 10-20 min in the centrifugal separation process.
3. The process for preparing the polypeptide organic fertilizer by using the blood of slaughtered livestock according to claim 1, wherein in the second step, the addition amount E/S of the endoprotease is 8000U/g.
4. The process for preparing the polypeptide organic fertilizer by using the blood of slaughtered livestock according to claim 1, wherein in the second step, ultrasonic hemolysis treatment is carried out by starting ultrasonic waves while carrying out preliminary enzymolysis for 25-35 min, wherein the ultrasonic frequency is 26-30 Khz, and the interval between the ultrasonic time and the intermittent time is 1: 1.
5. the process for preparing the polypeptide organic fertilizer by using the blood of slaughtered livestock according to claim 4, wherein the ultrasonic hemolysis treatment is carried out while adding the nonionic surfactant, wherein the ratio of the nonionic surfactant in the mixed solution is 0.2-1.0%, and the mass ratio of the nonionic surfactant is 1: 1 and polyoxyethylene.
6. The process for preparing a fertilizer for increasing the yield of slaughtered livestock and poultry blood according to claim 1, wherein the potassium sorbate accounts for 0.1% of the enzymolysis solution in the third step.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113200787A (en) * | 2021-06-23 | 2021-08-03 | 西北民族大学 | Amino acid liquid fertilizer and preparation method thereof |
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| CN105859874A (en) * | 2016-05-26 | 2016-08-17 | 陈石良 | Preparation method for producing pig haemocyte active small peptide powder through one-step method |
| CN110642649A (en) * | 2019-10-25 | 2020-01-03 | 眉县畜牧兽医技术推广站 | Process for preparing organic fertilizer by using animal blood |
| WO2020143205A1 (en) * | 2019-01-09 | 2020-07-16 | 广东省微生物研究所（广东省微生物分析检测中心） | Biological preparation method for producing amino acid liquid fertilizer with waste feathers |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105384522A (en) * | 2015-11-23 | 2016-03-09 | 重庆三零三科技有限公司 | Preparation method of selenium-rich protein peptide leaf fertilizer |
| CN105859874A (en) * | 2016-05-26 | 2016-08-17 | 陈石良 | Preparation method for producing pig haemocyte active small peptide powder through one-step method |
| WO2020143205A1 (en) * | 2019-01-09 | 2020-07-16 | 广东省微生物研究所（广东省微生物分析检测中心） | Biological preparation method for producing amino acid liquid fertilizer with waste feathers |
| CN110642649A (en) * | 2019-10-25 | 2020-01-03 | 眉县畜牧兽医技术推广站 | Process for preparing organic fertilizer by using animal blood |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113200787A (en) * | 2021-06-23 | 2021-08-03 | 西北民族大学 | Amino acid liquid fertilizer and preparation method thereof |
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Application publication date: 20210810 |