CN113215046A - Bacillus cereus WF01 and application thereof in removing suspended matters in water body - Google Patents
Bacillus cereus WF01 and application thereof in removing suspended matters in water body Download PDFInfo
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- CN113215046A CN113215046A CN202110549523.1A CN202110549523A CN113215046A CN 113215046 A CN113215046 A CN 113215046A CN 202110549523 A CN202110549523 A CN 202110549523A CN 113215046 A CN113215046 A CN 113215046A
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- bacillus cereus
- removing suspended
- water body
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- suspended matters
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- 241000193755 Bacillus cereus Species 0.000 title claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 238000004321 preservation Methods 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 239000006919 modified lb Substances 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 230000016615 flocculation Effects 0.000 abstract description 20
- 238000005189 flocculation Methods 0.000 abstract description 20
- 230000000694 effects Effects 0.000 abstract description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract description 5
- 239000000440 bentonite Substances 0.000 abstract description 5
- 229910000278 bentonite Inorganic materials 0.000 abstract description 5
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 abstract description 5
- 231100000956 nontoxicity Toxicity 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 7
- 239000008394 flocculating agent Substances 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000000701 coagulant Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 241000269335 Ambystoma laterale x Ambystoma jeffersonianum Species 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to bacillus cereus WF01, which is preserved in China center for type culture Collection with the preservation date of 2021, 3 months and 4 days and the preservation numbers of: CCTCC NO: m2021207, the preservation address is China, Wuhan university, postcode 430072. Application of bacillus cereus WF01 in removing suspended matters in water. The flocculant has good flocculation activity on diatomite, bentonite and loess body, has potential wide applicability, and has the advantages of safety, no toxicity, no secondary pollution and the like.
Description
Technical Field
The invention relates to the technical field of water treatment, in particular to Bacillus cereus (WF 01) and application thereof in removing suspended matters in water.
Background
The microbial flocculant is a substance with flocculation activity produced by microorganisms, and the main chemical components of the microbial flocculant are macromolecular substances such as polysaccharide, protein, lipid, nucleic acid and the like, so that the microbial flocculant has good flocculation and precipitation performance due to the specific structure of the macromolecular substances. Microbial flocculants have been reported to be used in the treatment research and engineering practice of various domestic wastewater, industrial wastewater and various heavy metal wastewater. Compared with traditional chemical flocculants such as inorganic high molecular polymers (polyaluminium chloride, polyaluminium sulfate or iron) and organic high molecular compounds (polyacrylamide), the microbial flocculant has the advantages of safety, no toxicity, no secondary pollution and the like, so that the development of the novel microbial flocculant has important value for removing suspended matters in water bodies.
Disclosure of Invention
The invention aims to solve the technical problem of providing a Bacillus cereus (Bacillus cereus) WF01 and application thereof in removing suspended matters in water, so as to overcome the defects in the prior art.
The technical scheme for solving the technical problems is as follows: bacillus cereus (Bacillus cereus) WF01 is preserved in China center for type culture Collection with the preservation date of 2021, 3 months and 4 days and the preservation numbers are as follows: CCTCC NO: m2021207, the preservation address is China, Wuhan university, postcode 430072.
On the basis of the technical scheme, the invention can be further improved as follows.
Application of Bacillus cereus (WF 01) in removing suspended matters in water body is provided.
An application of a fermentation liquid of Bacillus cereus (Bacillus cereus) WF01 in removing suspended matters in a water body is disclosed, wherein the culture method of the fermentation liquid comprises the following steps:
and inoculating the bacillus cereus WF01 into an improved LB culture medium for culture to prepare WF01 fermentation liquor.
Further, the improved LB culture medium comprises the following raw materials:
10g/L of glucose, 10g/L of peptone and 5g/L of yeast extract powder;
the pH is 7.0-7.5.
Further, the conditions for culturing Bacillus cereus WF01 in the modified LB medium were: cultured at 37 ℃ for 72 h.
The invention has the beneficial effects that: the flocculant has good flocculation activity on diatomite, bentonite and loess bodies, wherein the flocculation effect on a diatomite flocculation system can reach 89%, the flocculation effect on a bentonite flocculation system can reach 92%, the flocculation effect on a loess flocculation system can reach 81%, and the flocculant has the advantages of potential wide applicability, safety, no toxicity, no secondary pollution and the like.
Drawings
FIG. 1 shows a phylogenetic tree obtained by analyzing the 16S rDNA sequence of the strain WF 01.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
Bacillus cereus (Bacillus cereus) WF01 is preserved in China center for type culture Collection with the preservation date of 2021, 3 months and 4 days and the preservation numbers are as follows: CCTCC NO: m2021207, the preservation address is China, Wuhan university, postcode 430072.
Application of Bacillus cereus (WF 01) in removing suspended matters in water body is provided.
An application of a fermentation liquid of Bacillus cereus (Bacillus cereus) WF01 in removing suspended matters in a water body is disclosed, wherein the culture method of the fermentation liquid comprises the following steps:
and inoculating the bacillus cereus WF01 into an improved LB culture medium for culture to prepare WF01 fermentation liquor.
Wherein, the improved LB culture medium comprises the following raw materials:
10g/L of glucose, 10g/L of peptone and 5g/L of yeast extract powder; the pH is 7.0-7.5.
Further, the conditions for culturing Bacillus cereus WF01 in the modified LB medium were: cultured at 37 ℃ for 72 h.
Example (b):
confirming whether the bacillus cereus WF01 has the efficacy of removing the suspended matters in the water body:
1) bacillus cereus WF01, which is separated from soil and has the preservation number of (CCTCC NO:2021207), extracting the total DNA of WF01, determining the 16S rDNA sequence, constructing an evolutionary tree by using MEGA6.0, determining the species of the evolutionary tree, and identifying the strain WF01 as Bacillus cereus (Bacillus cereus);
inoculating bacillus cereus WF01 into an LB culture medium, wherein the LB culture medium comprises the following raw materials:
10g/L of glucose, 10g/L of peptone and 5g/L of yeast extract powder; the pH is 7.0-7.5;
culturing at 37 deg.C for 72h to obtain WF01 fermentation liquid;
culturing Bacillus subtilis (without flocculation activity) with the same culture medium under the same culture conditions to obtain a fermentation liquid as one of control groups;
2) comparison of flocculation effects of WF01 fermentation broth
Respectively weighing 0.3g of processed (dried, ground and sieved by a standard sieve of 80 meshes) diatomite (purchased), bentonite (purchased) and loess (collected from lion mountain of Huazhong agriculture university), placing in a 250mL triangular flask, adding distilled water to 100mL, and stirring uniformly to obtain suspension;
then adding 1mL WF01 fermentation broth, 1mL Bacillus subtilis fermentation broth, 10ppm Polyacrylamide (PAM), 10ppm polyaluminium chloride (PAC), 10ppm polyferric chloride (PFC), and 0.1mL 1% CaCl2Adjusting the pH value of the metal coagulant aid to 7.0-7.5, respectively measuring the flocculation activity of the metal coagulant aid after reacting for 20min, and comparing the flocculation effects of WF01 on non-flocculation microorganisms (bacillus subtilis) and 3 traditional flocculants on different flocculation systems;
3) method for comparing flocculation effect
Taking the suspension liquid after flocculation reaction, measuring OD value at wavelength of 550nm, standing for 20min, and taking the suspension liquid without flocculant as a control group;
flocculation activity ═ A-B)/(Ax 100%
Wherein: a is the OD value of the control group suspension at the wavelength of 550nm, and B is the OD value of the experimental group suspension at the wavelength of 550 nm;
compared with other traditional flocculants, the WF01 fermentation liquor has good flocculation activity on diatomite, bentonite and loess, has no difference compared with the traditional 3 flocculants, and even has more advantages to a certain extent, so that the microbial flocculant has potential wide applicability.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Sequence listing
<110> Mitsu Wu design research Co., Ltd
<120> Bacillus cereus WF01 and application thereof in removing suspended matters in water body
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1109
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
gcgggtgcta tacatgcagt cgagcgaatg gattaagagc ttgctcttat gaagttagcg 60
gcggacgggt gagtaacacg tgggtaacct gcccataaga ctgggataac tccgggaaac 120
cggggctaat accggataac attttgaacc gcatggttcg aaattgaaag gcggcttcgg 180
ctgtcactta tggatggacc cgcgtcgcat tagctagttg gtgaggtaac ggctcaccaa 240
ggcaacgatg cgtagccgac ctgagagggt gatcggccac actgggactg agacacggcc 300
cagactccta cgggaggcag cagtagggaa tcttccgcaa tggacgaaag tctgacggag 360
caacgccgcg tgagtgatga aggctttcgg gtcgtaaaac tctgttgtta gggaagaaca 420
agtgctagtt gaataagctg gcaccttgac ggtacctaac cagaaagcca cggctaacta 480
cgtgccagca gccgcggtaa tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa 540
agcgcgcgca ggtggtttct taagtctgat gtgaaagccc acggctcaac cgtggagggt 600
cattggaaac tgggagactt gagtgcagaa gaggaaagtg gaattccatg tgtagcggtg 660
aaatgcgtag agatatggag gaacaccagt ggcgaaggcg actttctggt ctgtaactga 720
cactgaggcg cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780
aaacgatgag tgctaagtgt tagagggttt ccgcccttta gtgctgaagt taacgcatta 840
agcactccgc ctggggagta cggccgcaag gctgaaactc aaaggaattg acgggggccc 900
gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960
gacatcctct gacaacccta gagatagggc ttctccttcg ggagcagagt gacaggtggt 1020
gcatggttgc cgtcagctcg tgtcgggaga tgttgggtta agtcccgcaa cgagcgcaac 1080
ccttgatctt agttgccatc attaaattg 1109
Claims (5)
1. The bacillus cereus WF01 is preserved in China center for type culture Collection with the preservation date of 2021, 3 months and 4 days and the preservation numbers are as follows: CCTCC NO: m2021207, the preservation address is China, Wuhan university, postcode 430072.
2. The use of a bacillus cereus WF01 as claimed in claim 1 for removing a suspension in a body of water.
3. The use of the fermentation broth of bacillus cereus WF01 for removing suspended substances from a water body according to claim 1, wherein the fermentation broth is cultured by the following steps:
and inoculating the bacillus cereus WF01 into an improved LB culture medium for culture to prepare WF01 fermentation liquor.
4. The use of the fermentation broth of bacillus cereus WF01 for removing suspended matter in a water body according to claim 3, wherein the modified LB medium comprises the following raw materials:
10g/L of glucose, 10g/L of peptone and 5g/L of yeast extract powder;
the pH is 7.0-7.5.
5. The use of the fermentation broth of bacillus cereus WF01 for removing suspended substances from a water body according to claim 4, wherein the conditions for culturing the bacillus cereus WF01 in the modified LB medium are as follows: cultured at 37 ℃ for 72 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110549523.1A CN113215046A (en) | 2021-05-20 | 2021-05-20 | Bacillus cereus WF01 and application thereof in removing suspended matters in water body |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110549523.1A CN113215046A (en) | 2021-05-20 | 2021-05-20 | Bacillus cereus WF01 and application thereof in removing suspended matters in water body |
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| Publication Number | Publication Date |
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| CN113215046A true CN113215046A (en) | 2021-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110549523.1A Pending CN113215046A (en) | 2021-05-20 | 2021-05-20 | Bacillus cereus WF01 and application thereof in removing suspended matters in water body |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106430633A (en) * | 2016-12-11 | 2017-02-22 | 湖南科技大学 | Bacillus cereus water heavy metal manganese removing method |
| CN111268805A (en) * | 2020-01-21 | 2020-06-12 | 中国科学院成都生物研究所 | Bacillus cereus and application thereof in pig manure biogas slurry flocculation |
-
2021
- 2021-05-20 CN CN202110549523.1A patent/CN113215046A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106430633A (en) * | 2016-12-11 | 2017-02-22 | 湖南科技大学 | Bacillus cereus water heavy metal manganese removing method |
| CN111268805A (en) * | 2020-01-21 | 2020-06-12 | 中国科学院成都生物研究所 | Bacillus cereus and application thereof in pig manure biogas slurry flocculation |
Non-Patent Citations (2)
| Title |
|---|
| MAO YL等: "Production of a novel biopolymer by culture of Bacillus cereus B-11 using molasses wastewater and its use for dye removal", 《ADVANCED MATERIALS RESEARCH》 * |
| 元王涛等: "絮凝菌JMH 48的鉴定及其产絮凝剂条件的优化研究", 《基因组学与应用生物学》 * |
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