CN113214992A

CN113214992A - Multifunctional cell culture device

Info

Publication number: CN113214992A
Application number: CN202110649987.XA
Authority: CN
Inventors: 常亮; �田�浩; 任妲妮; 李姣; 闫一芳; 焦利萍; 闫丽盈; 乔杰
Original assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Current assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Priority date: 2021-06-10
Filing date: 2021-06-10
Publication date: 2021-08-06
Anticipated expiration: 2041-06-10
Also published as: CN113214992B

Abstract

The invention discloses a multifunctional cell culture device, which comprises: the box body, the box body is provided with the base including the box bottom that sets up from bottom to top and mutually support, baffle and lid, the bottom of box bottom, be provided with a plurality of holes of placing that are used for holding the blake bottle on the base, be provided with a plurality of body that are used for supplying the blake bottle on the baffle and pass and with place the first through-hole that the hole matches, be provided with a plurality of second through-holes that are used for injecting experimental reagent in the blake bottle and correspond with the bottleneck position of blake bottle on the lid. According to the multifunctional cell culture device, the culture bottles are accommodated through the placing holes, the culture bottles are fixed through the first through holes, the experimental reagents are dripped through the second through holes without opening the culture bottles one by one or opening the box cover for dripping, the culture bottles are ensured to be always in a closed state in the process from inoculation to harvest, and aseptic, quick and one-stop inoculation is realized; the switch is convenient, the access is simple, the storage space is saved, and the operation time of cell inoculation and harvesting is shortened.

Description

Multifunctional cell culture device

Technical Field

The invention relates to the technical field of biological equipment, in particular to a multifunctional cell culture device.

Background

The chromosome specimen prepared by culturing the human peripheral blood lymphocytes has important biological and clinical significance, provides an experimental system for researching lymphocyte characteristics, pathophysiology and a cancer generating mechanism, and has important clinical significance for chromosome karyotype analysis and prenatal diagnosis.

The sterile state is required to be ensured from blood cell inoculation, culture to harvest, and the cells are required to be harvested simply, conveniently and quickly, so that the quality consistency of a large number of samples is ensured. In the existing experiment system, a special sterile convenient culture box does not exist, and the conditions required by inoculation and culture of a large number of clinical samples cannot be met, for example, consumables in the inoculation process of a blood sample are not sterile, and infection is easy to occur; through cell culture, before cell harvest, when the colchicine is dripped, the aluminum foil caps of the culture bottles need to be opened one by one, and the consumed time is long under the condition of more samples.

Therefore, a multifunctional cell culture device is needed.

Disclosure of Invention

The invention aims to provide a multifunctional cell culture device, which solves the problems in the prior art and can realize aseptic, quick and one-stop inoculation.

The present invention provides a multifunctional cell culture device, comprising:

the box body, the box body includes box bottom, baffle and lid that set up from bottom to top and mutually support the bottom of box bottom is provided with the base be provided with a plurality of holes of placing that are used for holding the blake bottle on the base, be provided with a plurality of confessions that are used for on the baffle the body of blake bottle pass and with place the first through-hole that the hole matches, be provided with on the lid a plurality of be used for to in the blake bottle inject experiment reagent and with the second through-hole that the bottleneck position of blake bottle corresponds.

In the multifunctional cell culture device, it is preferable that the two sides of the box body are provided with a snap structure for fixing the culture bottle, and the snap structure comprises a snap convex part on the box cover, a snap concave part on the partition plate, and a snap groove on the box bottom, and the snap convex part and the snap concave part are engaged and fixed and then embedded into the snap groove.

The multifunctional cell culture device as described above, wherein preferably, the bottom of the case, and/or the lid of the case, and/or the partition are made of transparent hard plastic.

The multifunctional cell culture device as described above, wherein the base is preferably made of sponge.

The multifunctional cell culture device as described above, wherein preferably a plurality of said culture bottles are distributed in a matrix in said cassette.

The multifunctional cell culture device as described above, wherein, preferably, the length of the base is 254mm-304mm, the width is 134mm-160mm, the height is 18mm-22mm, the diameter of the placement hole is 26mm-30mm, the distance between the center of the placement hole at the edge of the base and the side closer to the base is 22mm-26mm, the depth of the placement hole is 13mm-17mm, and the interval between two adjacent placement holes is 4mm-6 mm.

The multifunctional cell culture device as described above, wherein preferably, the length of the box cover is 258mm to 308mm, the width is 138mm to 164mm, the height is 35mm to 39mm, the wall thickness is 1.5mm to 2.5mm, the diameter of the second through hole is 8mm to 12mm, the distance between the center of the second through hole at the edge of the box cover and the side edge closer to the box cover is 22mm to 26mm, and the interval between two adjacent second through holes is 22mm to 24 mm; the length of the box bottom is consistent with that of the box cover, the width of the box bottom is consistent with that of the box cover, the height of the box bottom is 20mm-24mm, and the wall thickness of the box bottom is 1.5mm-2.5 mm.

The multifunctional cell culture device as described above, wherein preferably, the partition plate has a length corresponding to that of the box cover, a width corresponding to that of the box cover, and a height of 16mm to 20mm, the first through holes have a diameter of 22mm to 26mm, the center of the first through hole at the edge of the partition plate is 22mm to 26mm from the side closer to the partition plate, and the interval between two adjacent first through holes is 8mm to 10 mm.

As above-mentioned multi-functional cell culture device, wherein, preferably, multi-functional cell culture device is still including being used for holding the aseptic bag of box body, multi-functional cell culture device is still including setting up decapping mechanism in the aseptic bag, decapping mechanism is in including decapping chi, setting on the decapping chi and along a plurality of decapping nails and the cover of vertical distribution establish a plurality ofly decapping protective sheath on the nail, and decapping nail is in the distribution on the decapping chi with the blake is in the position in the box body is unanimous.

The multifunctional cell culture device as described above, wherein the length of the decapping ruler is 123mm-147mm, the width is 18mm-22mm, and the thickness is 4mm-6mm, the number of decapping rulers is consistent with the number of columns of culture bottles, the number of decapping nails on each decapping ruler is consistent with the number of rows of culture bottles, the decapping ruler has a start end and a tail end which are oppositely arranged, the start end is provided with a grasping portion, each decapping nail has a diameter of 0.5mm-1.5mm and a length of 85mm-95mm, the decapping nail is embedded into the decapping ruler and has a length of 3mm-7mm, the distance between two adjacent decapping nails is 30mm-34mm, and the decapping nail farthest from the grasping portion is 3mm-7mm from the tail end of the decapping ruler, the distance between the bottle opening nail nearest to the gripping part and the starting end of the bottle opening ruler is 28mm-32 mm; the length of the protective sleeve is 98mm-122mm, the width is 18mm-22mm, and the height is 90mm-100 mm.

The invention provides a multifunctional cell culture device, which is characterized in that a culture bottle is accommodated through a placing hole in a base, the culture bottle is fixed through a first through hole in a partition plate, an experimental reagent is dripped through a second through hole in a box cover, aluminum foil caps of the culture bottle do not need to be opened one by one, the box cover does not need to be opened for dripping, the culture bottle can be ensured to be always in a closed state in the whole process from inoculation to harvest, and aseptic, quick and one-stop inoculation can be realized; and the switch is convenient, the access is simple, the storage space is saved, the operation time of cell inoculation and harvesting can be shortened, and the cell inoculation and harvesting speed is greatly improved.

Drawings

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described with reference to the accompanying drawings, in which:

FIG. 1 is a schematic view of the overall structure of an embodiment of the multifunctional cell culture apparatus provided by the present invention;

FIG. 2 is a schematic structural view of a cassette bottom of an embodiment of the multifunctional cell culture apparatus provided in the present invention;

FIG. 3 is a schematic structural diagram of a base of an embodiment of the multifunctional cell culture apparatus provided in the present invention;

FIG. 4 is a schematic view showing the structure of a partition plate of an embodiment of the multi-functional cell culture apparatus according to the present invention;

FIG. 5 is a schematic structural diagram of a cover of a multifunctional cell culture device according to an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of an open bottle mechanism of the embodiment of the multifunctional cell culture apparatus provided by the invention;

FIG. 7 is a schematic structural diagram of a protective cover of an embodiment of the multifunctional cell culture device provided by the invention.

Description of reference numerals:

1-case bottom 2-clapboard 3-case cover

4-base 5-placing hole 6-first through hole

7-second through hole 8-snap structure 81-snap protrusion

82-buckling concave part 83-clamping groove 9-bottle opening mechanism

91-bottle opening ruler 92-bottle opening nail 93-protective sleeve

94-grip part

Detailed Description

Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative and is in no way intended to limit the disclosure, its application, or uses. The present disclosure may be embodied in many different forms and is not limited to the embodiments described herein. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that: the relative arrangement of parts and steps, the composition of materials, numerical expressions and numerical values set forth in these embodiments are to be construed as merely illustrative, and not as limitative, unless specifically stated otherwise.

As used in this disclosure, "first", "second": and the like, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element preceding the word covers the element listed after the word, and does not exclude the possibility that other elements are also covered. "upper", "lower", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

In the present disclosure, when a specific component is described as being located between a first component and a second component, there may or may not be intervening components between the specific component and the first component or the second component. When it is described that a specific component is connected to other components, the specific component may be directly connected to the other components without having an intervening component, or may be directly connected to the other components without having an intervening component.

All terms (including technical or scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate.

The invention provides a multifunctional cell culture device capable of realizing sterile, quick and one-stop inoculation. As shown in fig. 1, an embodiment of the present invention provides a multifunctional cell culture apparatus, including:

the box body comprises a box bottom 1 (shown in figure 2), a partition plate 2 (shown in figure 4) and a box cover 3 (shown in figure 5) which are arranged from bottom to top and are matched with each other, wherein a base 4 (shown in figure 3) is arranged at the bottom of the box bottom 1, as shown in figures 1 and 3, a plurality of placing holes 5 used for containing culture bottles are formed in the base 4, as shown in figures 1 and 4, a plurality of first through holes 6 used for enabling bottle bodies of the culture bottles to penetrate through and matched with the placing holes 5 are formed in the partition plate 2, as shown in figures 1 and 5, and a plurality of second through holes 7 used for injecting experiment reagents into the culture bottles and corresponding to bottle opening positions of the culture bottles are formed in the box cover 3.

The culture bottle is made of glass material, and the box bottom 1, and/or the box cover 3, and/or the partition plate 2 are made of transparent hard plastic, so that the state of cells in the culture bottle can be observed conveniently. Preferably, in the present invention, the case bottom 1, the case cover 3 and the partition 2 are made of polyphenylene sulfide (PPS).

Furthermore, the base 4 is made of sponge, which is beneficial to fixing the culture bottle, ensures the stability and firmness in the cell inoculation process, and avoids the pollution caused by the slippage of the culture bottle; meanwhile, the temperature change in the cell harvesting process is slowed down, and the good growth state of the cells is maintained.

In operation, after the blood cell inoculation to the blake bottle, demolish the aluminium foil cap on the blake bottle, then put into the blake bottle and set up in placing hole 5 on base 4, then overlap baffle 2 in blake bottle body department, buckle lid 3 at last, put into the incubator and cultivate, after the cultivation is accomplished, take out the box body, use syringe dropwise add experimental reagent (for example for colchicine) in second through-hole 7 department (the rubber cap department that exposes the blake bottle promptly) of lid 3, put into the incubator again and cultivate, take out the box body after the cultivation finishes. The culture bottle can be fixed through the first through hole 6 on the partition board 2 so as to realize stable operation; through the second through hole 7 on the box cover 3, when harvesting cells, the box cover 3 does not need to be opened, and the experimental reagent (such as colchicine) is dripped at the position of the second through hole 7 by using a syringe.

Therefore, compared with the prior art, the culture bottle is accommodated through the placing hole in the base, the culture bottle is fixed through the first through hole in the partition plate, the experimental reagent is dripped through the second through hole in the box cover, the aluminum foil caps of the culture bottle do not need to be opened one by one, the box cover does not need to be opened for dripping, the culture bottle can be ensured to be always in a closed state in the whole process from inoculation to harvest, and aseptic, quick and one-stop inoculation can be realized; and the switch is convenient, the access is simple, the storage space is saved, the operation time of cell inoculation and harvesting can be shortened, and the cell inoculation and harvesting speed is greatly improved.

Furthermore, the two sides of the box body are provided with a snap structure 8 for fixing the culture bottle, and the snap structure 8 comprises a snap convex part 81 (see fig. 5) on the box cover 3, a snap concave part 82 (see fig. 4) on the partition board 2 and a snap groove 83 (see fig. 2) on the box bottom 1, and the snap convex part 81 and the snap concave part 82 are embedded into the snap groove 83 after being snapped and fixed. Through buckle structure 8, can fix the blake bottle of placing in the box, prevent to rock the body when taking blake bottle, inoculation cell and dropwise add experimental reagent (for example for colchicine).

Further, a plurality of the culture bottles are distributed in the box body in a matrix, in the invention, the number of the culture bottles in the box body is 32, the culture bottles are distributed in a matrix with 4 rows and 8 columns, and correspondingly, the placing holes 5, the first through holes 6 and the second through holes 7 are also distributed in a matrix with 4 rows and 8 columns. The number and distribution of the culture bottles, the placement holes 5, and the first and second through

holes

6 and 7 are not particularly limited. The clapboard 2 adopts a special structural design, which is beneficial to realizing stable inoculation.

Specifically, the length of the base 4 is 254mm to 304mm, such as 279mm, the width is 134mm to 160mm, such as 147mm, the height is 18mm to 22mm, such as 20mm, the diameter of the placement hole 5 is 26mm to 30mm, such as 28mm, the distance between the center of the placement hole 5 at the edge of the base 4 and the side closer to the base 4 is 22mm to 26mm, such as 24mm, the depth of the placement hole 5 is 13mm to 17mm, such as 15mm, and the distance between two adjacent placement holes 5 is 4mm to 6mm, such as 5 mm. In the present invention, the placing holes 5 are blind holes, and it should be noted that the size of the base 4 and the size, number and distribution of the placing holes 5 are not particularly limited in the present invention.

Further, the length of the box cover 3 is 258mm to 308mm, such as 283mm, the width is 138mm to 164mm, such as 151mm, the height is 35mm to 39mm, such as 37mm, the wall thickness is 1.5mm to 2.5mm, such as 2mm, the diameter of the second through hole 7 is 8mm to 12mm, such as 10mm, the distance between the center of the second through hole 7 at the edge of the box cover 3 and the side edge closer to the box cover 3 is 22mm to 26mm, such as 24mm, and the interval between two adjacent second through holes 7 is 22mm to 24mm, such as 23 mm; the length of the box bottom 1 is the same as that of the box cover 3, such as 283mm, the width is the same as that of the box cover 3, such as 151mm, the height is 20mm-24mm, such as 22mm, and the wall thickness is 1.5mm-2.5mm, such as 2 mm. In concrete realization, the distribution of second through-hole 7 and the position one-to-one of the bottleneck of blake bottle to when cell inoculation and dropwise add colchicine to the blake bottle, need not to open lid 3 and directly can directly operate the rubber cap of blake bottle bottleneck department in second through-hole 7 department, convenient easy operation. It should be noted that the size of the box cover 3 and the box bottom 1 and the size, number and distribution of the second through holes 7 are not particularly limited in the present invention.

Further, the length of the partition board 2 is the same as the length of the box cover 3, such as 283mm, the width is the same as the width of the box cover 3, such as 151mm, the height is 16mm to 20mm, such as 18mm, the diameter of the first through hole 6 is 22mm to 26mm, such as 24mm, the distance between the center of the first through hole 6 at the edge of the partition board 2 and the side closer to the partition board 2 is 22mm to 26mm, such as 24mm, and the distance between two adjacent first through holes 6 is 8mm to 10mm, such as 9 mm. When using, with 2 cover of baffle to the body department of blake bottle, further fixed body, the body that prevents when the bottle lid is opened rocks and blood cell suspension is excessive, avoids taking place sample pollution and nosocomial infection. The size of the separator 2 and the size, number, and distribution of the first through holes 6 are not particularly limited in the present invention.

Further, multi-functional cell culture device still includes and is used for holding the aseptic bag of box body, as shown in fig. 6, multi-functional cell culture device still includes the setting and is in decapping mechanism 9 in the aseptic bag, decapping mechanism 9 includes decapping chi 91, sets up on decapping chi 91 and along a plurality of decapping nails 92 and the cover of vertical distribution establish a plurality of protective sheath 93 on decapping nail 92 (see fig. 7), and decapping nail 92 in the distribution on decapping chi 91 with the blake is in the position of box body is unanimous.

Specifically, the length of the bottle opening ruler 91 is 123mm-147mm, such as 135mm, the width is 18mm-22mm, such as 20mm, the thickness is 4mm-6mm, such as 5mm, the number of the bottle opening ruler 91 is consistent with the number of columns of the culture bottles, in the present invention, the number of the bottle opening ruler is 8, the number of the bottle opening nails 92 on each bottle opening ruler 91 is consistent with the number of rows of the culture bottles, in the present invention, the number of the bottle opening nails 92 on each bottle opening ruler 91 is four, and the bottle opening nails 92 are made of metal, the bottle opening ruler 91 has a starting end and a tail end which are oppositely arranged, the starting end is provided with a gripping part 94, each bottle opening nail 92 has a diameter of 0.5mm-1.5mm, such as 1mm, and a length of 85mm-95mm, such as 90mm, wherein the length of the bottle opening nail 92 embedded in the bottle opening ruler 91 is 3mm-7mm, for example, 5mm, the spacing between two adjacent bottle opening nails 92 is 30mm to 34mm, for example, 32mm, the distance between the bottle opening nail 92 farthest from the grasping portion 94 and the end of the bottle opening ruler 91 is 3mm to 7mm, for example, 5mm, and the distance between the bottle opening nail 92 closest to the grasping portion 94 and the starting end of the bottle opening ruler 91 is 28mm to 32mm, for example, 30 mm; the protective sleeve 93 has a length of 98mm to 122mm, for example 110mm, a width of 18mm to 22mm, for example 20mm, and a height of 90mm to 100mm, for example 95 mm. Through protective sheath 93, can protect uncork nail 92, not only clean but also can prevent sharp ware injury. The dimensions of the bottle opening rule 91, the bottle opening nail 92, and the protective cover 93 are not particularly limited in the present invention.

Wherein the decapping pins 92 are used to open the rubber caps of the culture flasks at the time of final harvesting of the cells. In concrete implementation, take out the box body after the end of culture in the incubator, open lid 3, then use decapping mechanism 9 can once open the rubber cap of a train of blake bottle (for example, be 4), open the completion back totally, transfer the cell suspension to the centrifuging tube in, follow-up reserve. In another embodiment of the present invention, the number of the decapping ruler 91 is equal to the number of rows of the culture bottles, and the number of the decapping pins 92 on each decapping ruler 91 is equal to the number of columns of the culture bottles. The rubber caps of one row of culture bottles can be opened at a time using the bottle opening mechanism 9.

At present, after colchicine is dripped to take effect, the cells are harvested, and samples between a culture bottle and a centrifugal tube are transferred, and rubber caps are still required to be opened one by one, so that the time cost is greatly increased, and the colchicine processing time of a first sample and a last sample is greatly different, so that the quality of the samples is not uniform, and the clinical diagnosis result is possibly influenced. According to the invention, through the bottle opening mechanism 9, the rubber caps of a plurality of culture bottles can be opened at one time after the culture is finished, so that the time cost is saved, the working strength is reduced, the operation error is avoided, the quality uniformity of the experimental sample is further improved, and the reliability of the clinical result is further ensured.

According to the multifunctional cell culture device provided by the embodiment of the invention, the culture bottles are accommodated through the placing holes in the base, the culture bottles are fixed through the first through holes in the partition plate, the experimental reagents are dripped through the second through holes in the box cover, the aluminum foil caps of the culture bottles do not need to be opened one by one, the box cover does not need to be opened for dripping, the culture bottles can be ensured to be always in a closed state in the whole process from inoculation to harvest, and aseptic, quick and one-stop inoculation can be realized; and the switch is convenient, the access is simple, the storage space is saved, the operation time of cell inoculation and harvesting can be shortened, and the cell inoculation and harvesting speed is greatly improved.

Thus, various embodiments of the present disclosure have been described in detail. Some details that are well known in the art have not been described in order to avoid obscuring the concepts of the present disclosure. It will be fully apparent to those skilled in the art from the foregoing description how to practice the presently disclosed embodiments.

Although some specific embodiments of the present disclosure have been described in detail by way of example, it should be understood by those skilled in the art that the foregoing examples are for purposes of illustration only and are not intended to limit the scope of the present disclosure. It will be understood by those skilled in the art that various changes may be made in the above embodiments or equivalents may be substituted for elements thereof without departing from the scope and spirit of the present disclosure. The scope of the present disclosure is defined by the appended claims.

Claims

1. A multifunctional cell culture device, comprising:

2. The multifunctional cell culture device according to claim 1, wherein the box body is provided at both sides thereof with snap structures for fixing the culture bottle, and the snap structures comprise a snap protrusion on the box cover, a snap recess on the partition, and a snap groove on the box bottom, and the snap protrusion and the snap recess are engaged with the snap groove after being snap-fixed.

3. The multifunctional cell culture device according to claim 1, wherein the bottom of the case, and/or the cover of the case, and/or the partition are made of transparent hard plastic.

4. The multifunctional cell culture device according to claim 1, wherein the base is made of sponge.

5. The multifunctional cell culture device according to claim 1, wherein a plurality of the culture bottles are distributed in a matrix in the cartridge body.

6. The multifunctional cell culture device according to claim 5, wherein the base has a length of 254mm to 304mm, a width of 134mm to 160mm, and a height of 18mm to 22mm, the placement holes have a diameter of 26mm to 30mm, the placement holes are positioned at the edge of the base at a distance of 22mm to 26mm from the center of the placement holes to the side closer to the base, the placement holes have a depth of 13mm to 17mm, and the interval between two adjacent placement holes is 4mm to 6 mm.

7. The multifunctional cell culture device according to claim 6, wherein the length of the box cover is 258mm to 308mm, the width is 138mm to 164mm, the height is 35mm to 39mm, the wall thickness is 1.5mm to 2.5mm, the diameter of the second through hole is 8mm to 12mm, the distance between the center of the second through hole at the edge of the box cover and the side closer to the box cover is 22mm to 26mm, and the interval between two adjacent second through holes is 22mm to 24 mm; the length of the box bottom is consistent with that of the box cover, the width of the box bottom is consistent with that of the box cover, the height of the box bottom is 20mm-24mm, and the wall thickness of the box bottom is 1.5mm-2.5 mm.

8. The multifunctional cell culture device according to claim 7, wherein the partition plate has a length corresponding to the length of the box cover, a width corresponding to the width of the box cover, and a height of 16mm to 20mm, wherein the first through holes have a diameter of 22mm to 26mm, the center of the first through hole at the edge of the partition plate is 22mm to 26mm from the side closer to the partition plate, and the interval between two adjacent first through holes is 8mm to 10 mm.

9. The multifunctional cell culture device according to claim 8, further comprising a sterile bag for accommodating the box body, and further comprising a decapping mechanism disposed in the sterile bag, wherein the decapping mechanism comprises a decapping ruler, a plurality of decapping nails disposed on the decapping ruler and vertically distributed, and a protective sleeve sleeved on the decapping nails, and the distribution of the decapping nails on the decapping ruler is consistent with the positions of the culture bottles in the box body.

10. The multifunctional cell culture device according to claim 9, wherein the number of the decapping ruler is equal to the number of columns of culture bottles, the number of the decapping nails on each decapping ruler is equal to the number of rows of culture bottles, the decapping ruler has a starting end and a tail end which are oppositely arranged, the starting end is provided with a grasping part, each decapping nail has a diameter of 0.5mm to 1.5mm and a length of 85mm to 95mm, the decapping nail is embedded in the decapping ruler and has a length of 3mm to 7mm, the distance between two adjacent decapping nails is 30mm to 34mm, and the decapping nail farthest from the grasping part is 3mm to 7mm from the tail end of the decapping ruler, the distance between the bottle opening nail nearest to the gripping part and the starting end of the bottle opening ruler is 28mm-32 mm; the length of the protective sleeve is 98mm-122mm, the width is 18mm-22mm, and the height is 90mm-100 mm.