CN113207886A

CN113207886A - Application of benconazole serving as strigolactone inhibitor

Info

Publication number: CN113207886A
Application number: CN202110407622.6A
Authority: CN
Inventors: 肖浪涛; 苏益; 寻敏
Original assignee: Hunan Agricultural University
Current assignee: Hunan Agricultural University
Priority date: 2021-04-15
Filing date: 2021-04-15
Publication date: 2021-08-06
Anticipated expiration: 2041-04-15
Also published as: CN113207886B

Abstract

The invention discloses application of benzyl oxazole as a strigolactone inhibitor, wherein the benzyl oxazole can be used as a plant strigolactone inhibitor for promoting plant branching, has an obvious yield-increasing effect on plants, can be applied to various plants, has small environmental pollution, is low in price of a benzyl oxazole reagent, and can be applied to agricultural production in a large scale.

Description

Application of benconazole serving as strigolactone inhibitor

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to application of benzofenapyr as a strigolactone inhibitor.

Background

Strigolactone (SL) was originally a sesquiterpene compound found when analyzing a signal substance secreted by plants in the corn root system that stimulates the germination of the seed of the malignant parasitic weed striga asiatica, and is therefore called strigolactone. Later proved to be a novel plant hormone ubiquitous in plants and having various physiological functions. Strigolactone has the ability to induce seed germination of both striga and broomrape root parasitic plants, such as the germination and parasitism of parasitic weeds. Due to its functional characteristics, strigolactone becomes a new means for regulating plant branching, directly or indirectly inhibits the lateral bud of the plant from sprouting to generate branches, can cooperate with auxin and cytokinin to control the quantity of the branches of the plant, regulate the configuration of the plant on the ground and underground, inhibit the branches of the plant above the ground, and influence the growth of root system, the elongation of root hair, the formation of root nodules of leguminous plants and the photosystem efficiency of the plant. The strigolactone has important functions for plant to adapt to environment and control plant growth process. Therefore, in the production of pesticides, the application of the inhibitor of the strigolactone to flowers and fruit trees can be used for promoting the branching of plants by utilizing the physical properties of the strigolactone, so that the effects of more blossoms and fruits, avoiding diseases in the pruning process, saving labor consumption and the like can be achieved. However, the existing strigolactone inhibitors have few types and high price, and are not suitable for large-scale application in agricultural production.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides the application of the benazol as the strigolactone inhibitor, which can effectively promote plant branching.

According to a first aspect of the present invention, there is provided the use of bifonazole as a strigolactone inhibitor.

A method of promoting branching in a plant, the method comprising the steps of: the above-mentioned solution of benzoprazole is sprayed on the plants.

In some embodiments of the present invention, the solvent for preparing the solution of benzoxyazole is at least one selected from the group consisting of absolute ethanol, distilled water, acetone, toluene, and xylene.

In some embodiments of the invention, the concentration of the sprayed benzypazole is 10 to 90. mu. mol/L.

In some embodiments of the invention, the concentration of the sprayed benzypazole is 30-60 μmol/L.

In some embodiments of the invention, the amount of the sprayed benzypazole solution is 1 to 10ml per strain.

In some embodiments of the invention, the method further comprises adding or not adding a bactericide, insecticide, acaricide, nematicide, herbicide, plant growth regulator, fertilizer or soil conditioner to the solution of benzoxap.

In some embodiments of the invention, the plant may include, but is not limited to, maize, rice, wheat, barley, rye, oats, sorghum, cotton, soybean, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugarcane, tobacco, arabidopsis.

A method of increasing seed yield in a plant, said method comprising the steps of: the solution of benzoprazole is sprayed onto the plants.

A method of growing a stress-resistant plant, the method comprising the steps of: the solution of benzoprazole is sprayed onto the plants.

The application of the benconazole serving as the strigolactone inhibitor has at least the following beneficial effects: the benconazole of the scheme of the invention can be used as a plant strigolactone inhibitor for promoting plant branching, has obvious yield-increasing effect on plants, can be applied to various plants, has little pollution to the environment, and the benconazole reagent has low price, can be applied in agricultural production on a large scale, achieves the effects of promoting plant branching, achieving multiple blossoms and multiple fruits, improving the stress resistance of plants, avoiding diseases caused in the pruning process and saving labor consumption.

Drawings

The invention is further described with reference to the following figures and examples, in which:

FIG. 1 is a graph showing the results of the total yield of 5 Arabidopsis thaliana plants treated with different concentrations of the benzpyrole reagent in example 1 of the present invention;

FIG. 2 is a graph showing the effect of different concentrations of benzpyrazole on the growth of Arabidopsis thaliana in example 2 of the present invention;

FIG. 3 is a graph showing the effect of different concentrations of the benzoxap reagent on the plant height of Arabidopsis thaliana in example 2 of the present invention;

FIG. 4 is a graph showing the effect of different concentrations of the benzoxap reagent on the photosynthetic intensity of Arabidopsis thaliana in example 3 of the present invention.

Detailed Description

The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments to fully understand the objects, features and effects of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and those skilled in the art can obtain other embodiments without inventive effort based on the embodiments of the present invention, and all embodiments are within the protection scope of the present invention. The test methods used in the examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are commercially available reagents and materials unless otherwise specified.

Preparing a benazol solution: 0.0403g of the raw benazol is taken, dissolved in absolute ethyl alcohol and then fixed to 10ml by using distilled water to prepare 10mM benazol mother liquor. When in use, the mother liquor is respectively diluted into 10, 30, 60 and 90 mu M of the benzoxap reagent.

Example 1 Effect of spray concentration of Bispyridazol on seed set percentage in Arabidopsis thaliana

The experiment was repeated three times, and the procedure for each repetition was as follows:

(1) and (4) preparing materials. Arabidopsis thaliana was used as a model plant.

(2) Seed selection and disinfection. The method comprises the following steps of: selecting Columbia wild type arabidopsis seeds, soaking and disinfecting the seeds for 15min by using 75% ethanol; and (5) washing with the sterilized deionized water to obtain the arabidopsis disinfection seeds.

(3) And (5) growing seedlings. And (3) dibbling the arabidopsis thaliana seeds obtained in the step (2) on 1/2MS solid culture medium, putting the arabidopsis thaliana seeds into a 4 ℃ refrigerator for vernalization for 3d, then putting the arabidopsis thaliana seeds into a 22 ℃ refrigerator for cultivation for 7d under 16h illumination and 8h dark conditions, transplanting the arabidopsis thaliana seeds into small pots (specification: 10cm multiplied by 10cm) for cultivation when 4 leaves grow out, and planting 5 plants in each pot.

(4) And (4) treating the benconazole reagent. When the arabidopsis is about to bolt, selecting arabidopsis with consistent growth vigor, taking every six small pots as a group, respectively spraying reagents (10, 30, 60 and 90 mu M) with different concentrations on each group, and respectively spraying arabidopsis with clear water as a control group. Spraying the pesticide once every 3 days for three times until the pesticide liquid naturally slides off the leaves (3 ml is sprayed on each plant). One treatment for each concentration, 5 arabidopsis thaliana plants were sprayed per treatment.

And (5) detecting seed setting rate. After the arabidopsis seeds are mature, the total yield of the arabidopsis seeds sprayed with the benzoxap reagents with different concentrations is respectively counted.

TABLE 1

The experimental results are shown in fig. 1 and table 1, fig. 1 shows the total yield of 5 arabidopsis thaliana seeds treated by different concentrations of the solution of the fenchlorazole, and table 1 shows the total yield of 25 arabidopsis thaliana seeds treated by different concentrations of the solution of the fenchlorazole, and it can be seen from fig. 1 and table 1 that after the solution of the fenchlorazole is sprayed, the total yield of arabidopsis thaliana treated by the solution of the fenchlorazole is improved to a certain extent compared with a control group, wherein the total yield of 90 μ M arabidopsis thaliana is improved by about 33% compared with the control group, and the yield of arabidopsis thaliana seeds is obviously increased by spraying the solution of the fenchlorazole.

Example 2 Effect of spray concentration of Bispyridazol on branching in Arabidopsis thaliana

The experimental steps for the influence of the spray concentration of the benconazole on the branches of the arabidopsis thaliana are as follows:

(2) Seed selection and disinfection. The method comprises the following steps of: selecting Columbia wild type arabidopsis seeds, soaking and disinfecting the seeds for 15min by using 75% ethanol; and (5) washing with sterilized deionized water for 3-5 times to obtain the arabidopsis disinfection seeds.

Measurement of plant height of Arabidopsis thaliana: arabidopsis thaliana sprayed with different concentrations of the benzoxap reagent for different times (7d, 14d) is shown in FIG. 2, and the length of the main stem from the rosette leaf to the tip of the inflorescence of Arabidopsis thaliana at different times (3d, 6d, 9d, 12d, 15d, 18d, 21d) is measured as shown in FIG. 3.

Measurement of the length of the Arabidopsis secondary branch: the average number of secondary branches on the main stem and the length from the beginning to the tip of the inflorescence of Arabidopsis thaliana at 9d after spraying with different concentrations of the benzypazole reagent were measured, and the results are shown in Table 2.

TABLE 2

The length of the main stem sprayed with different concentrations of the solution of the benazol is shown in figure 3, from which it can be seen that the solution of the benazol with high concentration has obvious promoting effect on the growth of the main stem, the results of the number and the length of the second-order branches of the arabidopsis thaliana are shown in table 2, from which it can be seen that when the solution of the benazol with the concentration of 10 μ M is sprayed, the number of the secondary branches and the average length of the secondary branches of Arabidopsis have no promotion effect basically relative to the control group, which indicates that the concentration of the benzoprazole reagent of 10 MuM and the concentration of the benzoprazole reagent of less than 10 MuM can not promote the branching of plants, after the benzypazole reagent of 30 mu M-60 mu M is sprayed, the number of the second-level branches and the average length of the second-level branches of the arabidopsis thaliana are obviously increased compared with the control group, after spraying 90 μ M of the benazol reagent, the average total secondary branch length was doubled compared to the control group, the spraying of the solution of the benazolum has obvious promotion effect on the branch number and the branch length of the arabidopsis thaliana.

Example 3 Effect of spray concentration of Bispyridazol on growth of Arabidopsis thaliana

The arabidopsis plants of the small group sprayed with different concentrations of the benconazole reagent (10, 30, 60, 90 μ M) in example 1 and the control group CK one week later were tested and the photosynthetic intensity thereof was measured under different concentration treatments.

Photosynthetic intensity determination: and (3) determining and outputting the photosynthetic intensity index by using the LI-COR/LI-6400 portable photosynthesis measuring system.

The determination result of the photosynthetic intensity is shown in fig. 4, and it can be seen from the figure that after the benconazole reagent is sprayed for one week, the net photosynthetic intensity of the arabidopsis thaliana treated by each treatment is increased compared with that of a control group, which indicates that the spraying of the benconazole reagent is beneficial to the accumulation of the growth amount of the arabidopsis thaliana, and meanwhile, the stress resistance of the arabidopsis thaliana is effectively improved and the adaptability of the plant to the environment is improved by spraying the benconazole reagent.

In conclusion, the scheme of the invention sprays the benzoxyazole reagent on the arabidopsis thaliana, and the benzoxyazole reagent is used as the inhibitor of strigolactone, so that the branching of the arabidopsis thaliana is effectively promoted, the yield of seeds is increased, and the stress resistance of plants is improved.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.

Claims

1. Use of benconazole as a strigolactone inhibitor.

2. A method for promoting branching in a plant, said method comprising the steps of: the solution of benzoprazole is sprayed onto the plants.

3. The method according to claim 2, wherein the solvent for preparing the solution of benzoxyazole is at least one selected from the group consisting of absolute ethanol, distilled water, acetone, toluene, and xylene.

4. The method according to claim 2, wherein the concentration of the solution of benzoxap is 10 to 90 μmol/L.

5. The method according to claim 2, wherein the concentration of the solution of benzoxap is 30-60 μmol/L.

6. The method as claimed in claim 2, wherein the amount of the solution of benzoprazole sprayed is 1 to 10ml per strain.

7. The method according to claim 2, further comprising adding or not adding a bactericide, insecticide, acaricide, nematicide, herbicide, plant growth regulator, fertilizer or soil conditioner to the solution of benzoxap.

8. The method of claim 2, wherein the plant may include, but is not limited to, maize, rice, wheat, barley, rye, oats, sorghum, cotton, soybean, peanut, buckwheat, sugar beet, rapeseed, sunflower, sugarcane, tobacco, Arabidopsis.

9. A method of increasing seed yield, comprising the steps of: the solution of benzoprazole is sprayed onto the plants.

10. A method of growing stress-resistant plants, comprising the steps of: the solution of benzoprazole is sprayed onto the plants.