CN113198330A - Long-life column chromatography head membrane for medical separation and purification and preparation process thereof - Google Patents
Long-life column chromatography head membrane for medical separation and purification and preparation process thereof Download PDFInfo
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- CN113198330A CN113198330A CN202110625627.6A CN202110625627A CN113198330A CN 113198330 A CN113198330 A CN 113198330A CN 202110625627 A CN202110625627 A CN 202110625627A CN 113198330 A CN113198330 A CN 113198330A
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- 239000012528 membrane Substances 0.000 title claims abstract description 63
- 238000000926 separation method Methods 0.000 title claims abstract description 31
- 238000000746 purification Methods 0.000 title claims abstract description 25
- 238000004440 column chromatography Methods 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000001746 injection moulding Methods 0.000 claims abstract description 12
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 238000010438 heat treatment Methods 0.000 claims description 26
- 238000001816 cooling Methods 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 19
- 239000011159 matrix material Substances 0.000 claims description 15
- 229920000642 polymer Polymers 0.000 claims description 15
- 238000002844 melting Methods 0.000 claims description 12
- 230000008018 melting Effects 0.000 claims description 12
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 11
- XRBCRPZXSCBRTK-UHFFFAOYSA-N phosphonous acid Chemical compound OPO XRBCRPZXSCBRTK-UHFFFAOYSA-N 0.000 claims description 11
- 229920002627 poly(phosphazenes) Polymers 0.000 claims description 11
- -1 polychlorosiloxane Polymers 0.000 claims description 11
- 230000000903 blocking effect Effects 0.000 claims description 10
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 150000001450 anions Chemical class 0.000 claims description 6
- 108010025899 gelatin film Proteins 0.000 claims description 6
- 125000000468 ketone group Chemical group 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 239000000741 silica gel Substances 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 6
- 150000002576 ketones Chemical class 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims 7
- 238000009434 installation Methods 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 4
- 238000004587 chromatography analysis Methods 0.000 abstract description 3
- 239000011521 glass Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229920000307 polymer substrate Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0002—Organic membrane manufacture
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0002—Organic membrane manufacture
- B01D67/002—Organic membrane manufacture from melts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/02—Inorganic material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/02—Inorganic material
- B01D71/024—Oxides
- B01D71/027—Silicium oxide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/06—Organic material
- B01D71/58—Other polymers having nitrogen in the main chain, with or without oxygen or carbon only
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Analytical Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention provides a long-life column chromatography head membrane for medical separation and purification and a preparation process thereof, wherein the membrane comprises a first base membrane and a second base membrane; the column head membrane is arranged in the column head membrane buckle, the inner ring part of the column head membrane buckle is provided with a fool-proof structure, and the fool-proof structure is tightly clamped in a connecting groove of a column head; according to the invention, through the original fool-proof design that the oblique angle is added at the inner ring part on the buckle, the buckle is only required to be installed on the column head, the fool-proof of the oblique angle can be tightly clamped in the connecting groove of the column head, the technical problem of falling and damage caused by error installation in use is solved, a parabolic semi-circular arc groove with the oblique angle of 15 degrees is machined at the connecting part of the buckle ring part on the existing injection mold core, the fool-proof structure of a fool-proof product can be directly formed through injection molding, the situation that a chromatography column head membrane falls and is damaged in the using process can be avoided, the accuracy of the installation position can be ensured, and the separation efficiency and the separation accuracy are greatly improved.
Description
Technical Field
The invention relates to the technical field of chromatographic columns, in particular to a long-life column chromatography head membrane for medical separation and purification and a preparation process thereof.
Background
The chromatographic column is a main body in the gel chromatography technology and generally adopts a glass tube or an organic glass tube. A chromatographic method for separating components in a sample mixture according to their different partition coefficients in a stationary phase and a mobile phase;
at present, a medical chromatographic column is the most commonly used separation device in the separation and purification industry, and a column head membrane in the chromatographic column is a key part for filtering liquid medicine, but the installation error is caused by human negligence in the use process, so that the medical chromatographic column falls down and is damaged or the filtration fails. In addition, the buckle strength of the column cap membrane and the material strength deviation of the column cap membrane seriously influence the quality and the service life of the chromatographic column.
Therefore, a long-life column chromatography head membrane for medical separation and purification and a preparation process thereof are provided.
Disclosure of Invention
In view of the above, embodiments of the present invention provide a long-life column head separation membrane for medical separation and purification and a preparation process thereof, so as to solve or alleviate the technical problems in the prior art, and at least provide a useful choice.
The technical scheme of the embodiment of the invention is realized as follows: a long-life column chromatography head membrane for medical separation and purification comprises a first basal membrane and a second basal membrane;
the first base film comprises the following components in parts by weight: 6-8 parts of polychlorosiloxane, 7-11 parts of polyphosphazene, 1.6-1.8 parts of carboxyl, 1.3-1.5 parts of phosphate group, 1.5-1.8 parts of phosphonous acid and 6-8 parts of ketone group;
the second base film is a silica gel film;
the column cap membrane is installed in column cap membrane buckle, and column cap membrane buckle inner circle position is provided with prevents slow-witted structure, prevents that slow-witted structure closely blocks in the spread groove of column cap.
In some embodiments: the fool-proof structure is a parabolic semi-circular arc plate with an oblique angle of 15 degrees.
A preparation process of a long-life column head separating membrane for medical separation and purification comprises the following steps:
s1, putting the polychlorosiloxane and the polyphosphazene into the first container, and heating and melting to obtain a polymer matrix;
s2, adding carboxyl, phosphate, phosphonite and ketone into the container II, and heating and melting to obtain a mixture of positively charged active groups and anions capable of moving on the active groups;
s3, additionally adding 2-3 second water outlet grooves at the first water outlet groove part of the die sleeve;
s4, putting the polymer matrix into the die sleeve, and then guiding the mixture into the die sleeve;
and S5, condensing to obtain a first base film, and mixing the first base film with a second base film to obtain the column cap film.
In some embodiments: in the step S1, the heating temperature of the first container is 60-80 ℃, and the heating time is 25-45 min.
In some embodiments: in the S2, the heating temperature of the second container is 70-90 ℃, and the heating time is 20-40 min.
In some embodiments: in S3, a blocking valve for controlling the flow rate is provided in the first outlet tank and the second outlet tank.
In some embodiments: the blocking valve is an electromagnetic valve and is connected with the controller, and the opening and closing of the electromagnetic valve are intelligently controlled.
In some embodiments: in the S4, the injection speed of the polymer matrix is 15-20ml/S, and after standing for 6-8min, the mixture is injected, wherein the injection speed of the mixture is 20-28 ml/S.
In some embodiments: in the step S5, when the die sleeve is condensed, the circulating water cooling device is adopted, flows outside the die sleeve, flows out of the die sleeve through the first water outlet groove and the second water outlet groove, enters the water cooling device, and then enters the circulating water cooling device, and is used for circulating water cooling to control the temperature of the die sleeve.
In some embodiments: before the column cap film is installed, a parabolic semi-arc groove with an oblique angle of 15 degrees is machined at the joint of the buckling ring part on the existing injection molding mold core.
Due to the adoption of the technical scheme, the embodiment of the invention has the following advantages:
firstly, the invention adds a bevel angle fool-proof design on the original buckle, only the buckle is arranged on the column head, the oblique angle fool-proof can be tightly clamped in the connecting groove of the column head, the technical problem of falling and damage caused by error installation in use is solved, a parabola semi-circular arc groove with a 15-degree bevel angle is machined on the connecting part of the buckle ring part on the existing injection mold core, the fool-proof structure of a fool-proof product can be directly formed by injection molding, the condition that a chromatography column head membrane falls and is damaged in the using process can be avoided, the accuracy of the installation position can be ensured, and the separation efficiency and accuracy are greatly improved.
The invention improves the column cap film injection molding process, avoids the situation that the buckle strength does not reach the standard due to overhigh temperature of a mold during injection molding, improves the buckle strength and prolongs the service life of a product.
The foregoing summary is provided for the purpose of description only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will be readily apparent by reference to the drawings and following detailed description.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments or technical descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of the preparation method of the present invention.
Detailed Description
In the following, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. Accordingly, the drawings and description are to be regarded as illustrative in nature, and not as restrictive.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example one
As shown in fig. 1, an embodiment of the present invention provides a long-life column chromatography head membrane for medical separation and purification, comprising a first base membrane and a second base membrane;
the first base film comprises the following components in parts by weight: 8 parts of polysiloxane chloride, 11 parts of polyphosphazene, 1.8 parts of carboxyl, 1.5 parts of phosphate group, 1.5 parts of phosphonous acid and 6 parts of ketone group;
the second base film is a silica gel film;
the column cap membrane is installed in column cap membrane buckle, and column cap membrane buckle inner circle position is provided with prevents slow-witted structure, prevents that slow-witted structure closely blocks in the spread groove of column cap.
In one embodiment: the fool-proof structure is a parabolic semi-circular arc plate with an oblique angle of 15 degrees; be used for improving the stability of column cap membrane installation in the glass pipe, prevent to lead to falling the technical problem of damage because of the mistake dress, improve life.
A preparation process of a long-life column head separating membrane for medical separation and purification comprises the following steps:
s1, putting the polychlorosiloxane and the polyphosphazene into the first container, and heating and melting to obtain a polymer matrix;
s2, adding carboxyl, phosphate, phosphonite and ketone into the container II, and heating and melting to obtain a mixture of positively charged active groups and anions capable of moving on the active groups;
s3, additionally adding 2 second water outlet grooves at the first water outlet groove part of the die sleeve;
s4, putting the polymer matrix into the die sleeve, and then guiding the mixture into the die sleeve;
and S5, condensing to obtain a first base film, and mixing the first base film with a second base film to obtain the column cap film.
In one embodiment: in S1, the first container is heated at 60 ℃ for 45 min.
In one embodiment: in S2, the heating temperature of the second container is 70 ℃, and the heating time is 40 min.
In one embodiment: in S3, a blocking valve for controlling the flow rate is provided in the first outlet tank and the second outlet tank.
In one embodiment: the blocking valve is an electromagnetic valve and is connected with the controller, and the opening and closing of the electromagnetic valve are intelligently controlled.
In one embodiment: in S4, the polymer matrix was injected at a rate of 15ml/S, and after standing for 6min, the mixture was injected at a rate of 20 ml/S.
In one embodiment: in S5, when the die sleeve is condensed, a circulating water cooling device is adopted to flow outside the die sleeve, flow out of the die sleeve through a first water outlet groove and a second water outlet groove, enter a water cooling device and then enter the circulating water cooling device, and the temperature of the die sleeve is controlled through circulating water cooling; the temperature is accurately controlled by increasing the water flow of the water outlet groove, and the problem that the strength of a column cap film cannot reach the standard due to the fact that the molecular structure of injection molding particles is changed due to overhigh mold temperature is solved.
In one embodiment: before mounting a column cap film, machining a parabolic semi-arc groove with an oblique angle of 15 degrees at the joint of a buckle ring part on the existing injection mold core; used for processing and forming the fool-proof structure.
Example two
As shown in fig. 1, an embodiment of the present invention provides a long-life column chromatography head membrane for medical separation and purification, comprising a first base membrane and a second base membrane;
the first base film comprises the following components in parts by weight: 7 parts of polysiloxane chloride, 9 parts of polyphosphazene, 1.7 parts of carboxyl, 1.4 parts of phosphate group, 1.7 parts of phosphonous acid and 7 parts of ketone group;
the second base film is a silica gel film;
the column cap membrane is installed in column cap membrane buckle, and column cap membrane buckle inner circle position is provided with prevents slow-witted structure, prevents that slow-witted structure closely blocks in the spread groove of column cap.
In one embodiment: the fool-proof structure is a parabolic semicircular arc plate with an oblique angle of 15 degrees.
A preparation process of a long-life column head separating membrane for medical separation and purification comprises the following steps:
s1, putting the polychlorosiloxane and the polyphosphazene into the first container, and heating and melting to obtain a polymer matrix;
s2, adding carboxyl, phosphate, phosphonite and ketone into the container II, and heating and melting to obtain a mixture of positively charged active groups and anions capable of moving on the active groups;
s3, additionally adding 3 second water outlet grooves at the first water outlet groove part of the die sleeve;
s4, putting the polymer matrix into the die sleeve, and then guiding the mixture into the die sleeve;
and S5, condensing to obtain a first base film, and mixing the first base film with a second base film to obtain the column cap film.
In one embodiment: in S1, the first container is heated at 70 deg.C for 35 min.
In one embodiment: in S2, the heating temperature of the second container is 80 ℃ and the heating time is 30 min.
In one embodiment: in S3, a blocking valve for controlling the flow rate is provided in the first outlet tank and the second outlet tank.
In one embodiment: the blocking valve is an electromagnetic valve and is connected with the controller, and the opening and closing of the electromagnetic valve are intelligently controlled.
In one embodiment: in S4, the polymer matrix was injected at a rate of 18ml/S, and after standing for 7min, the mixture was injected at a rate of 24 ml/S.
In one embodiment: in S5, when the die sleeve is condensed, the water cooling device is circulated, flows outside the die sleeve, flows out of the die sleeve through the first water outlet groove and the second water outlet groove, enters the water cooling device, and then enters the water cooling device, and the temperature of the die sleeve is controlled through circulating water cooling.
In one embodiment: before the column cap film is installed, a parabolic semi-arc groove with an oblique angle of 15 degrees is machined at the joint of the buckling ring part on the existing injection molding mold core.
EXAMPLE III
As shown in fig. 1, an embodiment of the present invention provides a long-life column chromatography head membrane for medical separation and purification, comprising a first base membrane and a second base membrane;
the first base film comprises the following components in parts by weight: 8 parts of polysiloxane chloride, 11 parts of polyphosphazene, 1.8 parts of carboxyl, 1.5 parts of phosphate group, 1.8 parts of phosphonous acid and 8 parts of ketone group;
the second base film is a silica gel film;
the column cap membrane is installed in column cap membrane buckle, and column cap membrane buckle inner circle position is provided with prevents slow-witted structure, prevents that slow-witted structure closely blocks in the spread groove of column cap.
In one embodiment: the fool-proof structure is a parabolic semicircular arc plate with an oblique angle of 15 degrees.
A preparation process of a long-life column head separating membrane for medical separation and purification comprises the following steps:
s1, putting the polychlorosiloxane and the polyphosphazene into the first container, and heating and melting to obtain a polymer matrix;
s2, adding carboxyl, phosphate, phosphonite and ketone into the container II, and heating and melting to obtain a mixture of positively charged active groups and anions capable of moving on the active groups;
s3, additionally adding 3 second water outlet grooves at the first water outlet groove part of the die sleeve;
s4, putting the polymer matrix into the die sleeve, and then guiding the mixture into the die sleeve;
and S5, condensing to obtain a first base film, and mixing the first base film with a second base film to obtain the column cap film.
In one embodiment: in S1, the first container is heated at 80 ℃ for 25 min.
In one embodiment: in S2, the heating temperature of the second container is 70 ℃, and the heating time is 20 min.
In one embodiment: in S3, a blocking valve for controlling the flow rate is provided in the first outlet tank and the second outlet tank.
In one embodiment: the blocking valve is an electromagnetic valve and is connected with the controller, and the opening and closing of the electromagnetic valve are intelligently controlled.
In one embodiment: in S4, the polymer matrix was injected at a rate of 20ml/S, and after standing for 8min, the mixture was injected at a rate of 28 ml/S.
In one embodiment: in S5, when the die sleeve is condensed, the water cooling device is circulated, flows outside the die sleeve, flows out of the die sleeve through the first water outlet groove and the second water outlet groove, enters the water cooling device, and then enters the water cooling device, and the temperature of the die sleeve is controlled through circulating water cooling.
In one embodiment: before the column cap film is installed, a parabolic semi-arc groove with an oblique angle of 15 degrees is machined at the joint of the buckling ring part on the existing injection molding mold core.
The invention is in operation: the preparation method comprises the steps of putting polychlorosiloxane and polyphosphazene into a container I, heating and melting to obtain a polymer substrate, matching carboxyl, phosphate, phosphonite and ketone groups, heating and melting to obtain a mixture of positively charged active groups and anions capable of moving on the active groups, forming a first base film which can purify protein and prolong the service life of a chromatographic column head film, matching a second base film (a silica gel film) to improve the service life of the chromatographic column head film, and adding a bevel angle foolproof design on an original buckle, namely, only installing the buckle on the column head, wherein the bevel angle foolproof design can be tightly clamped in a connecting groove of the column head, so that the technical problem of falling and damage caused by misassembly in use is solved, and a parabolic semicircular arc groove with a bevel angle of 15 degrees is machined at the connecting position of the buckle ring on the existing injection mold core, so that the foolproof structure of a foolproof product can be directly formed by injection molding, the problem that the buckle structure is difficult to demold is solved, two water outlet grooves are additionally arranged at the water outlet grooves of the die sleeve, and the water flow of the water outlet grooves is increased to accurately control the temperature, so that the problem that the strength of a column head membrane cannot reach the standard due to the fact that the molecular structure of injection particles is changed due to overhigh temperature of a die is solved, and the problem that the column head membrane is damaged due to falling in the using process can be avoided by adding the foolproof structure of the buckle, the accuracy of the installation position can be ensured, and the separation efficiency and the accuracy are greatly improved; the column cap film injection molding process is improved, the situation that the buckle strength does not reach the standard due to overhigh temperature of a mold during injection molding is avoided, and the strength of the buckle and the service life of a product are improved.
The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive various changes or substitutions within the technical scope of the present invention, and these should be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A long-life column chromatography head membrane for medical separation and purification, which is characterized by comprising a first base membrane and a second base membrane;
the first base film comprises the following components in parts by weight: 6-8 parts of polychlorosiloxane, 7-11 parts of polyphosphazene, 1.6-1.8 parts of carboxyl, 1.3-1.5 parts of phosphate group, 1.5-1.8 parts of phosphonous acid and 6-8 parts of ketone group;
the second base film is a silica gel film;
the column cap membrane is installed in column cap membrane buckle, and column cap membrane buckle inner circle position is provided with prevents slow-witted structure, prevents that slow-witted structure closely blocks in the spread groove of column cap.
2. The high lifetime column chromatography head membrane for medical separation and purification according to claim 1, wherein: the fool-proof structure is a parabolic semi-circular arc plate with an oblique angle of 15 degrees.
3. A preparation process of a long-life column head separating membrane for medical separation and purification is characterized by comprising the following steps:
s1, putting the polychlorosiloxane and the polyphosphazene into the first container, and heating and melting to obtain a polymer matrix;
s2, adding carboxyl, phosphate, phosphonite and ketone into the container II, and heating and melting to obtain a mixture of positively charged active groups and anions capable of moving on the active groups;
s3, additionally adding 2-3 second water outlet grooves at the first water outlet groove part of the die sleeve;
s4, putting the polymer matrix into the die sleeve, and then guiding the mixture into the die sleeve;
and S5, condensing to obtain a first base film, and mixing the first base film with a second base film to obtain the column cap film.
4. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 3, wherein: in the step S1, the heating temperature of the first container is 60-80 ℃, and the heating time is 25-45 min.
5. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 3, wherein: in the S2, the heating temperature of the second container is 70-90 ℃, and the heating time is 20-40 min.
6. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 3, wherein: in S3, a blocking valve for controlling the flow rate is provided in the first outlet tank and the second outlet tank.
7. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 6, wherein: the blocking valve is an electromagnetic valve and is connected with the controller, and the opening and closing of the electromagnetic valve are intelligently controlled.
8. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 3, wherein: in the S4, the injection speed of the polymer matrix is 15-20ml/S, and after standing for 6-8min, the mixture is injected, wherein the injection speed of the mixture is 20-28 ml/S.
9. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 3, wherein: in the step S5, when the die sleeve is condensed, the circulating water cooling device is adopted, flows outside the die sleeve, flows out of the die sleeve through the first water outlet groove and the second water outlet groove, enters the water cooling device, and then enters the circulating water cooling device, and is used for circulating water cooling to control the temperature of the die sleeve.
10. The process for preparing a long-life column chromatography head membrane for medical separation and purification according to claim 3, wherein: before the column cap film is installed, a parabolic semi-arc groove with an oblique angle of 15 degrees is machined at the joint of the buckling ring part on the existing injection molding mold core.
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