CN113197987A

CN113197987A - Preparation method and application of eye care solution

Info

Publication number: CN113197987A
Application number: CN202110494135.8A
Authority: CN
Inventors: 荆玉飞; 孙桂敏
Original assignee: Shandong Kaiman Biotechnology Co ltd
Current assignee: Shandong Kaiman Biotechnology Co ltd
Priority date: 2021-05-07
Filing date: 2021-05-07
Publication date: 2021-08-03

Abstract

The invention is suitable for the technical field of eye care, and provides a preparation method and application of an eye care solution, wherein the eye care solution is prepared by adopting a fermentation process, and the fermentation process adopts a natural fermentation method or a strain fermentation method; according to the embodiment of the invention, micromolecular components of traditional Chinese medicinal materials beneficial to eyes are extracted in a fermentation mode, and various enzymes generated in the microbial fermentation process are prepared into the eye care solution, so that the eye care solution has the effects of protecting the eyes and treating eye diseases, can restore and delay pathological changes in the middle and early stages of cataract, has the effect of delaying pathological changes in the middle and later stages of cataract, and solves the problem that the cataract is not treated effectively.

Description

Preparation method and application of eye care solution

Technical Field

The invention belongs to the technical field of eye care, and particularly relates to a preparation method and application of an eye care solution.

Background

Along with the continuous development of society, the electronic product industry of people is rapidly developed, people can feel uncomfortable and easily cause eye diseases by using electronic products for a long time, and the invention mainly aims at solving the problem of eye microcirculation. By using the eye care solution, the microcirculation of eyes can be effectively dredged, the eye care solution is mainly reflected in capillaries of eyes, lymphatic systems of eyes, lacrimal glands and the like, plays a role in eye health care, and has certain anti-inflammatory and bactericidal effects. The eye drop can play a role in preventing and treating dry eyes, unsmooth eyes, acid swelling of eyes, xerophthalmia, maculopathy, vitreous opacity, muscae volitantes, blurred vision, cataract, glaucoma and other eye diseases, as well as myopia, presbyopia and the like, has a remarkable treatment effect on cataract, plays a role in recovering and delaying the pathological changes in the middle and early stages of cataract, plays a role in delaying the pathological changes in the middle and later stages of cataract, and solves the worldwide problem that the cataract is not treated effectively.

Disclosure of Invention

The embodiment of the invention aims to provide a preparation method and application of an eye care solution, so as to solve the problems in the background technology.

In order to achieve the above object, an embodiment of the present invention provides a method for preparing an eye care solution, wherein the eye care solution is prepared by a fermentation process, the fermentation process adopts a natural fermentation method or a strain fermentation method, and the natural fermentation method comprises the following process flows: cleaning the fermentation product, mixing, fermenting, filtering, sterilizing and inactivating, diluting or not diluting, and packaging; the process flow of the strain fermentation method is as follows: cleaning the fermentation product, mixing, sterilizing, fermenting, filtering, sterilizing and inactivating, diluting or not diluting, and packaging.

Preferably, the natural fermentation method comprises the steps of:

(1) cleaning 1 weight part of the fermented product, mixing the fermented product with 1-10 weight parts of sugar, adding 1-100 weight parts of water, and performing mixed fermentation;

(2) controlling the fermentation temperature between 12 and 50 ℃ and fermenting for 28 to 200 days;

(3) filtering after fermentation is finished, reserving fermentation liquor, then sterilizing and inactivating, and extracting sterile fermentation liquor;

(4) the sterile fermentation liquid can be directly used or diluted with purified water; the ratio of the sterile fermentation liquor to the purified water is 1:1 to 1:1000, and then the sterile packaging is carried out.

Preferably, the strain fermentation method comprises the following steps:

(1) cleaning 1 part by weight of the fermented product, mixing the fermented product with 1-10 parts by weight of saccharides, adding 1-100 parts by weight of water, and mixing;

(2) performing comprehensive sterilization by adopting a high-temperature sterilization method before fermentation, and adding microbial bacteria for fermentation after reaching an aseptic state;

(3) controlling the fermentation temperature between 12 and 50 ℃ and fermenting for 28 to 200 days;

(4) filtering after fermentation is finished, reserving fermentation liquor, then sterilizing and inactivating, and extracting sterile fermentation liquor;

(5) the sterile fermentation liquid can be directly used or diluted with purified water; the ratio of the sterile fermentation liquor to the purified water is 1:1 to 1:1000, and then the sterile packaging is carried out.

Preferably, the fermentation product is one of the Chinese herbal medicines beneficial to eyes, such as alpinia lip, chrysanthemum, cassia seed, pine needle, ginkgo, liquorice, mint and the like.

Preferably, the sugars include, but are not limited to, honey, brown sugar, white sugar, granulated sugar, sucrose, glucose, molasses, and the like.

Preferably, the sterilization inactivation is performed by one of ionization inactivation, ultraviolet inactivation, or filtration inactivation.

Preferably, the microorganism bacteria is one or more of bacillus subtilis, streptococcus ferdii, lactobacillus reuteri, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium lactis, bifidobacterium adolescentis, lactobacillus rhamnosus and lactobacillus acidophilus.

The eye care solution prepared by the preparation method.

The application of the care solution in eye drops.

1. Small molecule technology:

molecules with molecular weights less than 500 bases are referred to as small molecules, which are generally simple monomeric species. Biologically, small molecules are bioactive small peptides, oligopeptides, oligosaccharides, oligonucleotides, vitamins, minerals, small molecular group water, plant secondary metabolites and their degradation products such as aglycones, xanthenes, aglycones, alkaloids, etc.

Natural small molecules are present in the nucleus and cytoplasm of eukaryotes, and their length is between 100 and 300 bases.

Two types of small-molecule RNA are mainly found in nature, one type is snRNA, and the small-molecule RNA exists in cell nucleus; the other is scRNA, present in the cytoplasm.

Small RNA molecules are often complexed with proteins and play an important role in the vital activities of the cell, with certain snRNPs and splicing roles closely related, which are complementary to the donor and acceptor splice sites and branching sequences, respectively. scRNA is involved in the synthesis and transport of proteins, for example, SRP particles are ribosomes composed of 7SRNA and protein, and the main function is to recognize signal peptide and guide ribosomes to endoplasmic reticulum.

The intake of external nutrition is the basis for human survival. The process of external nutrition digestion into nutrients in vivo is a macroscopic to microscopic system engineering. This process is essentially a process of small molecule formation, constituting a small molecule life line for nutrients. The small molecules are an important node of the life line of a human body, the number, the quality, the composition structure and the working efficiency of the small molecules determine the life and death of the human body, slight change of the skin is concerned if the small molecules are used, and the growth, the survival, the health, the service life and the final life of the human body are concerned if the small molecules are used.

The small molecule lifeline in the human body has inherent limitations, and is invaded to different degrees along with various artificial and non-artificial injuries. The damage of the small molecular life lines directly influences the nutrition of each system of the human body, all the systems are damaged, and the small molecular life lines form a multiplication effect with various existing pathological conditions of the human body, and generate new unhealthy effects and new pathological changes.

The biggest difference of the micromolecules relative to the macromolecules is that the micromolecules have small molecular weight and bioactivity, most of the micromolecules can be directly absorbed by a human body without being decomposed, and the absorption efficiency is greatly improved.

2. The biological enzyme technology comprises the following steps:

biological enzymes are catalytic organisms produced by living cells, and are mostly proteins and few RNAs. The biological enzyme is a nontoxic and environment-friendly biological catalyst, and the chemical nature of the biological enzyme is protein. The production and application of the enzyme have more than 80 years of history at home and abroad, and the production and application of the enzyme enter 80 years in the 20 th century, bioengineering is rapidly developed in China as a new high-tech technology, and the field of enzyme manufacture and application is gradually expanded.

Biological enzymes are proteins with catalytic functions. Like other proteins, enzyme molecules consist of long chains of amino acids. Wherein one part of the chain is in a spiral shape, and the other part is in a folded sheet structure, and the two parts are connected by the unfolded amino acid chain, so that the whole enzyme molecule is in a specific three-dimensional structure. The biological enzyme is produced from organism, has special catalytic function, and has the characteristics of high efficiency, wherein the enzyme is used as catalyst, and the catalytic efficiency of the enzyme is 10 of that of the general inorganic catalyst⁷-10¹³And (4) doubling.

Enzyme proteins differ from other proteins in that enzymes all have an active center. The enzyme can be divided into a quaternary structure, wherein the primary structure is the arrangement sequence of amino acids, the secondary structure is the planar space conformation of a peptide chain, the tertiary structure is the three-dimensional space conformation of the peptide chain, and the quaternary structure is that the peptide chains are combined with each other by non-covalent bonds to form a complete protein molecule. The true determinant is the primary structure of the enzyme, and its alteration will alter the properties of the enzyme (inactivation or denaturation).

Enzymes are classified into "lytic enzymes" and "synthetic enzymes" as a broad category. For example, proteins are decomposed into amino acids having a size that can be absorbed, and proteins necessary for the body are synthesized by decomposition enzymes and the absorbed amino acids, which are carried out by enzymes.

In the fermentation process of the present invention, the enzymes produced are mainly

1. Lysozyme: the lysozyme can be used as a natural anti-infection substance with bactericidal effect, and has the effects of resisting bacteria, relieving swelling, accelerating tissue recovery, etc.

2. Lipase: the lipase is an enzyme for decomposing natural oil and fat, can hydrolyze fat into glycerol and fatty acid, further carry out B oxidation on the fatty acid, remove one C2 product each time, generate acetyl COA (N-cyclohexyl octyl amine), enter a TCA (tricarboxylic acid) ring for complete oxidation or enter a glyoxylate ring for synthesizing saccharides.

3. Protease: the proteases secreted by microorganisms vary depending on the species, for example, subtilisin secretes gelatinase and casein, which hydrolyze gelatin and casein, and Streptomyces freudenreichii secretes keratinase, which hydrolyze keratin of animal hair, horn, and hoof. Proteases break down proteins into peptides, which are then hydrolyzed by peptidases into amino acids.

4. Cellulase: the cellulase is a multi-component enzyme system, wherein the cellobiohydrolase in the cellulase is also called exo-cellulase and consists of two enzymes of CHBI and CHBII, and the endoglucanase is also called endo-cellulase and consists of at least 5 cellulases (EGI, EGII, EGHI, EGIV and EGV). In addition, there is 13-glucuronase. These cellulases have a synergistic effect in the hydrolysis of cellulose.

5. Amylase: amylases are a generic term for enzymes that hydrolyze starch and glycogen, and generally act on α -1, 4-glucan such as soluble starch, amylose, and glycogen to hydrolyze α -1, 4-glucosidic bonds. It can be divided into alpha-amylase, beta 1-amylase, gamma-amylase and isoamylase according to the different isomerous types of enzyme hydrolysate.

In summary, due to the adoption of the technical scheme, the method has the following beneficial effects:

the embodiment of the invention provides a preparation method and application of an eye care solution, wherein micromolecular components of traditional Chinese medicinal materials beneficial to eyes are extracted in a fermentation mode, and various enzymes generated in a microbial fermentation process are prepared into the eye care solution, and the eye care solution is externally administrated in a mode of dropping into eyes, so that the eye care solution has the effects of protecting the eyes and treating eye diseases, can play a role in recovering and delaying pathological changes in the middle and later stages of cataract, and plays a role in delaying pathological changes in the middle and later stages of cataract.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention are described in further detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the embodiments of the invention and are not limiting of the embodiments of the invention.

At present, no effective treatment method exists for cataract, and the embodiment of the invention extracts micromolecule components of Chinese medicinal materials beneficial to eyes in a fermentation mode and various enzymes generated in a microbial fermentation process to prepare eye care solution, so that the eye care solution can play a role in recovering and delaying lesion in the middle and early stages of cataract and a role in delaying lesion in the middle and later stages of cataract.

Example 1

A preparation method of an eye care solution, which is prepared by a natural fermentation method, comprises the following steps:

(1) washing 10g of the fermented product, mixing the fermented product with 10g of the sugar, adding 10g of water, and performing mixed fermentation;

(2) controlling the fermentation temperature between 12 ℃ and fermenting for 28 days;

(4) the sterile fermentation liquid can be directly used or diluted with purified water; the ratio of the sterile fermentation liquor to the purified water is 1:1, and then the sterile packaging is carried out.

The fermentation product is Zingiber zerumbet Hance, the sugar is honey, and the sterilization and inactivation adopt an ionization inactivation method.

Example 2

(1) washing 10g of the fermented product, mixing the fermented product with 100g of sugar, adding 1000g of water, and performing mixed fermentation;

(2) controlling the fermentation temperature between 50 ℃ and fermenting for 200 days;

(4) the sterile fermentation liquid can be directly used or diluted with purified water; the ratio of the sterile fermentation liquor to the purified water is 1:1000, and then the sterile packaging is carried out.

The fermentation product is chrysanthemum, the sugar is honey, and the sterilization and inactivation adopt an ionization inactivation method.

Example 3

A preparation method of an eye care solution, wherein the eye care solution adopts a strain fermentation method, and comprises the following steps:

(1) washing 10g of the fermented product, mixing the fermented product with 10g of the saccharide, adding 10g of water, and mixing;

(3) controlling the fermentation temperature between 12 ℃ and fermenting for 28 days;

(5) the sterile fermentation liquid can be directly used or diluted with purified water; the ratio of the sterile fermentation liquor to the purified water is 1:1, and then the sterile packaging is carried out.

The fermentation product is chrysanthemum, the sugar is brown sugar, the sterilization inactivation is performed by adopting an ultraviolet inactivation method, and the microorganism is the Streptomyces freudenreichii.

Example 4

(1) washing 10g of the fermented product, mixing the fermented product with 100g of the saccharide, adding 1000g of water, and mixing;

(3) controlling the fermentation temperature between 40 ℃ and fermenting for 180 days;

(5) the sterile fermentation liquid can be directly used or diluted with purified water; the ratio of the sterile fermentation liquor to the purified water is 1:100, and then the sterile packaging is carried out.

The fermentation product is Zingiber zerumbet Hance, the sugar is honey, the sterilization and inactivation adopt an ionization inactivation method,

the microorganism bacterium is bacillus subtilis.

The product is dripped into eyes, and after the product is dripped, part of people can feel pain, granular sensation, astringent sensation and the like, which are normal phenomena; pain is caused, which is often the expression of inflammation in eyes, or poor circulation of capillary vessels in eyes and the like; the eye mask has granular sensation, and is often poor in ocular capillary circulation or poor in ocular lymphatic system metabolism and the like; astringency is usually manifested by obstruction of the lacrimal gland in the eye.

The product prepared in example 4 was tested and the results are shown in table 1.

TABLE 1

Typical cases are as follows:

case one: jingyufei, male, after myopic laser surgery performed in 11 months in 2012, muscae volitantes and dry eyes appeared in 4 months in 2013. After 5% eye care solution is used for one month, the dry eye symptom disappears, and after three months, the muscae volitantes is cured.

Case two: jing Ching, male, 61 years old, due to age and living habits, appeared dry eye and asthenopia symptoms, so using 5% eye care solution, about 1 week, dry eye symptom improved, and dry eye symptom disappeared after 20 days. Is now used for daily eye health care.

Case three: zhang a flower, female, 57 years old, after suffering from iridocyclitis, using eye care solution with concentration of 5%, the eye care solution is greatly relieved after three days, and basically heals after 5 days, and the eye care solution heals after 7 days.

Case four: the eye dryness is caused by lacrimal gland blockage in some Japanese apricot and female, and is disappeared by using 20 ml of eye care solution with the concentration of 30% for one month.

Case five: the product is suitable for women, 33 years old, and patients with conjunctivitis can be cured after 5 days with 5% glasses care solution.

Case six: sunzuo, a female, age 29, develops blurred vision due to improper use of the eyes. The eye care solution with the concentration of 5% is used for daily health care, the vision is blurred and improved after 2 days of use, and the vision is recovered to be normal after 25 days of use.

Case seven: liu Yi Yan, women, 47 years old, due to diabetes, eye complications appear, left eye vision is 0.1, right eye vision is 0.2, using 5% eye care solution for one month, using 10 ml, the vision is recovered to left eye 0.6, right eye 0.8.

Case eight: bear army, male, age 65, mild cataracts. 120 ml of eye care solution with the concentration of 30% is used, and after 9 months, the cataract basically disappears.

Case nine: liu Yi, female, 54 years old, in the early stage of glaucoma, 120 ml of eye care solution with the concentration of 30% is used, and after 9 months, the vision is rechecked to restore to normal.

Case ten: wangbaobo, male, 49 years old, fundus macular degeneration, using eye care solution with the concentration of 10%, using 90 ml, after 9 months, rechecking, macular degeneration disappears.

In summary, the following steps: according to the embodiment of the invention, micromolecular components of traditional Chinese medicinal materials beneficial to eyes are extracted in a fermentation mode, and various enzymes generated in a microbial fermentation process are prepared into the eye care solution, and the eye care solution is externally administrated by dropping into eyes, so that the eye care solution has the effects of protecting the eyes and treating eye diseases, can restore and delay pathological changes in the middle and early stages of cataract, and has the effect of delaying pathological changes in the middle and later stages of cataract.

It will be evident to those skilled in the art that the embodiments of the present invention are not limited to the details of the foregoing illustrative embodiments, and that the embodiments of the present invention are capable of being embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the embodiments being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The preparation method of the eye care solution is characterized in that the eye care solution is prepared by adopting a fermentation process, the fermentation process adopts a natural fermentation method or a strain fermentation method, and the process flow of the natural fermentation method is as follows: cleaning the fermentation product, mixing, fermenting, filtering, sterilizing and inactivating, diluting or not diluting, and packaging; the process flow of the strain fermentation method is as follows: cleaning the fermentation product, mixing, sterilizing, fermenting, filtering, sterilizing and inactivating, diluting or not diluting, and packaging.

2. The method of claim 1, wherein the natural fermentation process comprises the steps of:

3. The method of claim 2, wherein the fermentation process comprises the steps of:

4. The method of claim 3, wherein the fermentation product is one of the herbs beneficial to eyes such as Zingiber zerumbet, Chrysanthemum morifolium, Cassia obtusifolia, pine needle, Ginkgo biloba, Glycyrrhiza uralensis, and Mentha haplocalyx.

5. The method of claim 3, wherein the sugar includes, but is not limited to, honey, brown sugar, white sugar, granulated sugar, sucrose, glucose, molasses, and the like.

6. The method of claim 3, wherein the sterilizing is performed by one of ionization inactivation, UV inactivation, and filtration inactivation.

7. The method of claim 3, wherein the microorganism is one or more of Bacillus subtilis, Streptomyces Firmianae, Lactobacillus reuteri, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium lactis, Bifidobacterium adolescentis, Lactobacillus rhamnosus, and Lactobacillus acidophilus.

8. A solution prepared by the method of claim 7.

9. Use of a solution according to claim 7 in eye drops.