CN113151248B - Quick degradation method for perishable garbage and application thereof - Google Patents
Quick degradation method for perishable garbage and application thereof Download PDFInfo
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- CN113151248B CN113151248B CN202110356655.2A CN202110356655A CN113151248B CN 113151248 B CN113151248 B CN 113151248B CN 202110356655 A CN202110356655 A CN 202110356655A CN 113151248 B CN113151248 B CN 113151248B
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- 238000006731 degradation reaction Methods 0.000 title claims abstract description 132
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- 238000000034 method Methods 0.000 title claims abstract description 40
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 83
- 238000003756 stirring Methods 0.000 claims abstract description 64
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 10
- 239000007857 degradation product Substances 0.000 claims abstract description 8
- 239000002893 slag Substances 0.000 claims abstract description 6
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- 239000000243 solution Substances 0.000 claims description 71
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention relates to the technical field of perishable garbage treatment, in particular to a perishable garbage rapid degradation method and application thereof. Firstly, providing a degradation bin with a stirring rod inside, wherein the stirring rod can be driven by a stirring motor arranged outside the degradation bin, the top of the degradation bin is provided with a feeding port capable of feeding perishable garbage, and the bottom of the degradation bin is provided with a discharge port capable of discharging perishable garbage degradation products; a perishable garbage rapid degradation microbial inoculum is detachably fixed on the stirring rod; after non-degradable substances are picked out from the perishable garbage, crushing the perishable garbage to pass through a 10-mesh sieve, placing the sieved material in a degradation bin, starting a stirring motor to drive a stirring rod to rotate at 30-120 r/min, and carrying out aerobic degradation for 24-36 hours; after the degradation is finished, the degradation product is discharged through a discharge hole, and the slag and the liquid are separated and are further treated. The method can finish a large amount of degradation treatment on the perishable garbage in a short time, degrade the perishable garbage into water and carbon dioxide, and has no obvious peculiar smell in the degradation process.
Description
Technical Field
The invention relates to the technical field of perishable garbage treatment, in particular to a perishable garbage rapid degradation method and application thereof.
Background
The perishable garbage has high water content and low calorific value, and can not meet the calorific value requirement of garbage incineration power generation if being incinerated together with other industrial garbage, decocted garbage and the like, and toxic substances such as dioxin and the like generated when the perishable garbage with high organic matter content is incinerated can harm the health of people. The perishable garbage mainly comprises sugar, protein, starch, fat, cellulose, trace elements and the like, has the characteristics of high organic matter content, rich nutrition and easy decay and deterioration, is easy to cause stink in sanitary landfill, generates a large amount of percolate, methane and other pollutions, and seriously harms surface water, underground water, soil and air.
The invention discloses a microbial agent for quickly degrading and reducing organic garbage and application thereof in the prior art, wherein the microbial agent is prepared by mixing bacillus subtilis with the collection number of CGMCC No.14152, bacillus amyloliquefaciens with the collection number of CGMCC No.14053, bacillus subtilis with the collection number of CICC No.10210 and cellulose degrading bacteria with the collection number of CICC No. 10829. Mixing a microbial agent with carrier rice chaff, attaching the microbial agent to the carrier rice chaff, screening the organic garbage, and performing filter pressing treatment, and then throwing the carrier rice chaff attached with the microbial agent into the organic garbage for degradation. However, the organic garbage of the technical scheme needs to be subjected to filter pressing before degradation, the water content is controlled to be 25-35%, the percolate needs to be additionally treated, and the degraded waste liquid can be discharged after additional treatment.
In addition, the invention discloses a Chinese patent with an authorization publication number of CN105665417B in the prior art, and discloses a composite microbial agent for efficiently degrading kitchen waste and a preparation method and application thereof. The composite bacteria of the invention are fully mixed with the organic kitchen waste in the fermentation equipment, and under the aerobic condition at normal temperature, different strains act synergistically, so that the degradation rate of the organic kitchen waste is improved, the fermentation time is shortened, and meanwhile, the deodorization effect is obvious. However, the technical scheme has low degradation and reduction rate on the kitchen garbage, and the carrier can not be repeatedly applied.
The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.
Disclosure of Invention
Technical problem to be solved
The technical problem to be solved by the invention is as follows: the carrier has high adsorption rate to microorganisms, is easy to separate and extract after the perishable garbage is degraded, can repeatedly adsorb the microorganisms to prepare the degrading microbial inoculum after regeneration, reduces the preparation cost of the microbial inoculum, and solves the problems that the traditional microbial carrier is not easy to separate and can not be repeatedly used.
The invention also provides a method for rapidly degrading the perishable garbage, which can complete a large amount of degradation treatment on the perishable garbage in a short time, degrade the perishable garbage into water and carbon dioxide, and generate no obvious peculiar smell in the degradation process.
(II) technical scheme
In order to solve the above technical problems or to achieve the above technical object, the present invention provides the following technical solutions.
A preparation method of a recyclable carrier of a perishable garbage rapid degradation microbial inoculum comprises the following steps:
1) soaking the basalt fibers in an acetone solution for ultrasonic cleaning for at least 4 hours, washing with deionized water for 2-4 times, placing in a hydrochloric acid solution, soaking at 35 ℃ for at least 30min, taking out, washing to be neutral, drying, shearing to be 10-15 cm in length, taking 10-15 g of basalt fibers, and fastening one end of each basalt fiber to be umbrella-shaped;
2) preparing 1-2 g/L dopamine solution, then adding 30-50 mg/L populus euphratica white carbon black, and ultrasonically stirring for at least 1h to obtain dopamine suspension; adding a Tris buffer solution with the pH value of 7.2 into the dopamine suspension, adjusting the pH value to 7.8-8.0, and stirring for at least 30min to obtain a mixed solution;
3) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) in the mixed solution obtained in the step 2), continuously stirring and reacting for at least 4 hours, taking out, washing with deionized water until the washing solution is neutral, and drying to obtain the basalt fiber cluster.
The preparation method comprises the following limitations:
the diameter of the basalt fiber in the step 1) is 12-15 mu m; and/or
The mass fraction of the acetone solution in the step 1) is 5-20%; and/or
The ultrasonic frequency of the ultrasonic cleaning in the step 1) is 25-40 KHz, and the ultrasonic density is 0.3-0.6W/cm2(ii) a And/or
The molar concentration of the hydrochloric acid solution in the step 1) is 0.5-1.0 mol/L; and/or
The drying in the step 1) is drying for at least 1h at the temperature of 40-60 ℃; and/or
The populus diversifolia white carbon black in the step 2) is prepared by the following method: heating ash after burning of populus euphratica branches and/or leaves at 550-600 ℃ for 2 hours, dispersing the ash in a sodium hydroxide solution according to the mass ratio of 1.3-1.5: 1 of populus euphratica ash to sodium hydroxide, stirring and reacting at 45-60 ℃ with a cover at 120-600 r/min for at least 2 hours, filtering, washing residues with distilled water, settling, taking supernate, adjusting acid and alkali to be neutral, filtering with a 1500-mesh sieve, washing with deionized water, and drying at 40-60 ℃ for 48 hours to obtain the product; and/or
The ultrasonic stirring in the step 2) is carried out at the frequency of 25-40 KHz and the density of 0.3-0.6W/cm2(ii) a Stirring at a rotating speed of 120-600 r/min; and/or
The addition amount of the Tris buffer solution in the step 2) is 10-30% of the volume of the dopamine suspension; and/or
Adjusting the pH value by using 0.5mol/L hydrochloric acid solution and/or 0.5mol/L sodium hydroxide solution in the step 2); and/or
The stirring speed of the step 2) is 120-600 r/min; and/or
Soaking the basalt fiber cluster obtained in the step 3) into the suspension at a concentration of 50-150 g/L; and/or
And the drying in the step 3) is to dry at the temperature of 45-60 ℃ to constant weight.
In the method, the basalt fiber is soaked by acetone to remove slurry on the surface of the fiber, then the basalt fiber is soaked by warm acid liquor to carry out surface treatment on the fiber by an acid etching method so as to increase the surface roughness, finally dopamine and populus euphratica white carbon black are used for carrying out deep modification, a large number of phenolic hydroxyl groups and imino groups of polydopamine are grafted on the surface of the modified basalt fiber, the populus euphratica white carbon black can also be grafted on the surface of the fiber to form granular or blocky deposition on the surface of the fiber, the roughness of the surface of the basalt fiber is further improved, the increase of polar groups on the surface of the basalt and the increase of the roughness are beneficial to the attachment of microorganisms, and the verification shows that compared with other plant-source white carbon black, the populus euphratica white carbon black can be used for improving the adsorption effect of the carrier on the microorganisms to a greater extent and is beneficial to the enrichment of the microorganisms, so that the basalt fiber carrier prepared by the technical scheme of the application has excellent microorganism adsorption rate, can enrich microorganisms to a great extent, improve the content of the microorganisms in unit microbial inoculum and is beneficial to the rapid degradation of perishable garbage.
The invention also provides a recyclable carrier of the perishable garbage rapid degradation microbial inoculum prepared by the method.
The invention also provides a perishable garbage rapid degradation microbial inoculum based on the carrier, which comprises the following components:
the perishable garbage rapid degradation microbial inoculum can be recycled carrier, and
the composite microbial inoculum fixed in the carrier consists of bacillus amyloliquefaciens, nitrogen-producing pseudomonas, light yellow pseudomonas, pseudomonas brucei, pseudomonas winkle, saccharomycetes and actinomycetes.
The invention also provides a preparation method of the perishable garbage rapid degradation microbial inoculum, which specifically comprises the following steps: the content of the compound total strains is 1-2 multiplied by 1010cfu/mL of composite bacterial liquid; immersing 100-150 g/L of umbelliform basalt fiber carrier into the bacterial liquid, fully mixing, standing for 24-36 h, taking out, and drying for 30min at the temperature of below 40 ℃ to obtain the composite bacterial strain.
In the compound bacterial liquid, the content difference among all strains is not more than 50%.
Aiming at the characteristic of high organic matter content of perishable garbage, the inventor optimally configures a composite microbial agent consisting of bacillus amyloliquefaciens, pseudomonas azotoformis, pseudomonas yellowish pseudomonas, pseudomonas brucei, pseudomonas winkleinians, saccharomycetes and actinomycetes, prepares a rapid degradation microbial agent by immobilizing the composite microbial agent into the carrier, applies the rapid degradation microbial agent to the degradation of perishable garbage, can completely degrade more than 90% of perishable garbage into liquid and carbon dioxide within 24-36 h by high-efficiency microbial enzymes generated by microorganisms, discharges the liquid into a municipal sewage pipe network after reaching the standard through anaerobic treatment, can prepare and recycle the residual perishable garbage into biological fertilizer, and has the advantages of high degradation speed, low unit energy consumption, high treatment efficiency, low treatment cost and the like, thereby fundamentally solving the problem of perishable garbage treatment.
The invention also provides a method for rapidly degrading perishable garbage, which comprises the following steps:
1) providing a degradation bin with a stirring rod inside, wherein the stirring rod can be driven by a stirring motor arranged outside the degradation bin, the top of the degradation bin is provided with a feeding port capable of feeding perishable garbage, and the bottom of the degradation bin is provided with a discharge port capable of discharging perishable garbage degradation products;
2) detachably fixing the prepared perishable garbage rapid degradation microbial inoculum on the stirring rod;
3) after non-degradable substances are picked out from the perishable garbage, crushing the perishable garbage to pass through a 10-mesh sieve, placing the sieved material in a degradation bin, starting a stirring motor to drive a stirring rod to rotate at 30-120 r/min, and carrying out aerobic degradation for 24-36 hours; after the degradation is finished, the degradation product is discharged through a discharge hole, and the slag and the liquid are separated and are further treated.
The aforementioned degradation methods include the following limitations:
in the step 2), the perishable garbage rapid degradation microbial inoculum is arranged and replaced according to the amount of 1-2 kg/t/12 h; and/or
In the step 3), the aerobic degradation temperature is 15-40 ℃, and the relative humidity is 80-90%; and/or
In the step 3), ventilation is carried out for 8-10 times/h during aerobic degradation; and/or
And 3) in the step 3), after the slag-liquid separation, taking liquid, performing anaerobic treatment on the liquid to reach the standard, discharging the liquid into a municipal sewage pipe network, and preparing the biological fertilizer from the perishable garbage slag for recycling.
The compound composite microbial inoculum is optimized and immobilized in the modified basalt fiber carrier to obtain the rapid degradation microbial inoculum, so that the perishable garbage can be rapidly degraded, the decrement rate of more than 90 percent of the perishable garbage can be realized within 24-36 h, the perishable garbage is degraded into water and carbon dioxide, no obvious peculiar smell is generated in the degradation process, and the rapid degradation microbial inoculum is friendly to operators and environment; the rapid degradation microbial inoculum is fixed on a stirring rod of a degradation bin in a basalt fiber form, along with stirring and mixing of perishable garbage, microorganisms in a carrier are dispersed into the perishable garbage to generate high-efficiency microbial enzymes to degrade the perishable garbage, the rapid degradation microbial inoculum can be replaced every 12 hours, the degradation microbial inoculum is easy to replace, the replaced basalt fiber carrier can be repeatedly applied to adsorption of the microorganisms after being washed to prepare the rapid degradation microbial inoculum again, the adsorption rate of the basalt fiber carrier is still not lower than 70% during third utilization, and the preparation cost of the degradation microbial inoculum is greatly reduced.
The invention also provides application of the degradation method in rapid degradation of perishable garbage.
The above-described preferred conditions may be combined with each other to obtain a specific embodiment, in accordance with common knowledge in the art.
The raw materials or reagents involved in the invention are all common commercial products, and the operations involved are all routine operations in the field unless otherwise specified.
(III) advantageous effects
The technical scheme of the invention has the following advantages:
1) after the basalt fiber is modified, a large number of phenolic hydroxyl groups and imino groups are grafted on the surface of the basalt fiber, and the populus diversifolia white carbon black can be grafted on the surface of the fiber to form granular or blocky deposition on the surface of the fiber, so that the roughness of the surface of the basalt fiber is further improved, and the increase of polar groups and the increase of the roughness of the surface of the basalt fiber are beneficial to the attachment of microorganisms;
2) compared with other plant-derived white carbon black, the poplar white carbon black is selected to improve the adsorption effect of the carrier on microorganisms to a greater extent, and is beneficial to enrichment of the microorganisms, so that the basalt fiber carrier prepared by the technical scheme of the application has relatively excellent microorganism adsorption rate, can enrich the microorganisms to a greater extent, improves the content of the microorganisms in a unit microbial inoculum, and is beneficial to rapid degradation of perishable garbage;
3) the rapid degradation microbial inoculum is fixed on the stirring rod in the form of basalt fibers, the rapid degradation microbial inoculum can be replaced every 12 hours, the replaced basalt fiber carrier can be repeatedly applied to the adsorption of microorganisms after being washed so as to prepare the rapid degradation microbial inoculum again, the adsorption rate of the rapid degradation microbial inoculum is still not lower than 70% when the rapid degradation microbial inoculum is used for the third time, and the preparation cost of the degradation microbial inoculum is greatly reduced;
4) the composite microbial inoculum is optimally configured and immobilized in a carrier to prepare the rapid degradation microbial inoculum, more than 90% of perishable garbage can be completely degraded into water and carbon dioxide within 24-36 h by using the rapid degradation microbial inoculum, no obvious peculiar smell is generated in the degradation process, and the method has the advantages of high degradation speed, low unit energy consumption, high treatment efficiency, low treatment cost and the like, and fundamentally solves the problem of perishable garbage treatment.
The invention adopts the technical scheme for achieving the purpose, makes up the defects of the prior art, and has reasonable design and convenient operation.
Drawings
The foregoing and/or other objects, features, advantages and embodiments of the invention will be more readily understood from the following description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic diagram of a process for preparing a recyclable carrier according to the present invention;
FIG. 2 is a schematic view of the degradation apparatus of the present invention;
FIG. 3 is a schematic diagram of the reduction rate of the perishable waste degradation according to various embodiments of the present invention.
Description of reference numerals: 1. a degradation bin; 2. a stirring rod; 3. a feeding port; 4. a stirring motor; 5. a discharge outlet; 6. the perishable garbage can degrade the microbial inoculum rapidly.
Detailed Description
Those skilled in the art can appropriately substitute and/or modify the process parameters to implement the present disclosure, but it is specifically noted that all similar substitutes and/or modifications will be apparent to those skilled in the art and are deemed to be included in the present invention. While the products and methods of making described herein have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the products and methods of making described herein may be made and utilized without departing from the spirit and scope of the invention.
Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The present invention uses the methods and materials described herein; other suitable methods and materials known in the art may be used. The materials, methods, and examples described herein are illustrative only and are not intended to be limiting. All publications, patent applications, patents, provisional applications, database entries, and other references mentioned herein, and the like, are incorporated by reference herein in their entirety. In case of conflict, the present specification, including definitions, will control.
The materials, methods, and examples described herein are illustrative only and not intended to be limiting unless otherwise specified. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described herein.
The starting materials described herein are commercially available and include, but are not limited to:
bacillus amyloliquefaciens, nitrogen-producing pseudomonas, pseudomonas flavus, pseudomonas brucei, pseudomonas winkle and yeast are purchased from Beijing Beinana Chuanglian Biotechnology research institute;
actinomycetes were purchased from Hezhong Biochemical manufacturing Co., Ltd, Wuhan City.
The present invention is described in detail below.
Example 1: a perishable garbage rapid degradation microbial inoculum:
this embodiment first provides a recyclable carrier, as shown in fig. 1, which includes the following steps:
1) soaking basalt fiber (diameter 12 μm) in 5% acetone solution at frequency of 25KHz and density of 0.3W/cm2Ultrasonic cleaning for 6h under the condition, washing with deionized water for 2 times, soaking in 0.5mol/L hydrochloric acid solution at 35 deg.C for 60min, taking out, washing to neutral, drying at 40 deg.C for h, shearing into 10cm length, taking 15g basalt fiber, and fastening one end into umbrella shape;
2) heating ash after burning of populus euphratica branches at 550 ℃ for 2h, dispersing the ash in 0.2mol L sodium hydroxide solution according to the mass ratio of 1.3:1 of the populus euphratica ash to the sodium hydroxide, stirring and reacting for 6h at 45 ℃ with a cover of 120r/min, washing residues after filtering with distilled water, taking supernate after settling, adjusting acid and alkali to be neutral, filtering with a 1500-mesh sieve, washing with deionized water for 2 times, and drying at 40 ℃ for 48h to obtain white carbon black of populus euphratica;
3) preparing 1g/L dopamine solution, adding 30mg/L white carbon black obtained in the step 2), stirring for 3 hours at 120r/min, and adding 25KHz of frequency and 0.3W/cm of density2Obtaining dopamine suspension by ultrasonic treatment; adding a 10 volume percent Tris buffer solution with pH7.2 of the dopamine suspension into the dopamine suspension, adjusting the pH to 7.8 by using a 0.5mol/L hydrochloric acid solution and/or a 0.5mol/L sodium hydroxide solution, and stirring at 120r/min for 60min to obtain a mixed solution;
4) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) into the mixed solution obtained in the step 3) in an amount of 50g/L, continuously stirring for reaction for 6 hours at a speed of 120r/min, taking out, washing with deionized water until the washing solution is neutral, and drying at a temperature of 45 ℃ to constant weight.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum, which comprises the reusable carrier and a composite microbial inoculum which is fixed in the carrier and consists of bacillus amyloliquefaciens, pseudomonas azotoformis, pseudomonas flavus, pseudomonas brucei, pseudomonas winkle, yeast and actinomycetes; the preparation method specifically comprises the following steps:
is compounded by 2 x 109cfu/mL Bacillus amyloliquefaciens, 2X 109cfu/mL Pseudomonas azotoformans, 2X 109cfu/mL Pseudomonas flaccid, 2X 109cfu/mL Pseudomonas brucei, 2X 109cfu/mL Pseudomonas wrinkle, 2.5X 109cfu/mL yeast and 2.5X 109A compound bacterium liquid consisting of cfu/mL actinomycetes; immersing 100g/L of recyclable carrier into the bacterial liquid, fully mixing, standing for 24h, taking out, and drying for 30min at 35 ℃.
Example 2: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) soaking basalt fiber (diameter 15 μm) in 20% acetone solution at frequency of 40KHz and density of 0.6W/cm2Ultrasonic cleaning for 4h under the condition, washing with deionized water for 4 times, soaking in 1.0mol/L hydrochloric acid solution at 35 deg.C for 30min, taking out, washing to neutral, drying at 60 deg.C for 1h, shearing into 15cm length, taking 10g basalt fiber, and fastening one end into umbrella shape;
2) heating ash after burning of populus diversifolia leaves at 600 ℃ for 2h, dispersing the ash in 0.1mol/L sodium hydroxide solution according to the mass ratio of 1.5:1 of the populus diversifolia ash to the sodium hydroxide, stirring and reacting for 2h at the temperature of 60 ℃ with a cover of 600r/min, washing residues after filtering with distilled water, taking supernate after settling, adjusting the pH value to be neutral, filtering with a 1500-mesh sieve, washing with deionized water, and drying for 48h at 60 ℃ to obtain the populus diversifolia white carbon black;
3) preparing 2g/L dopamine solution, adding 50mg/L white carbon black obtained in the step 2), stirring for 1h at 600r/min, and adding 40KHz of frequency and 0.6W/cm of density2Obtaining dopamine suspension by ultrasonic treatment; adding a 30 volume percent Tris buffer solution with pH7.2 of the dopamine suspension into the dopamine suspension, adjusting the pH to 8.0 by using a 0.5mol/L hydrochloric acid solution and/or a 0.5mol/L sodium hydroxide solution, and stirring at 600r/min for 30min to obtain a mixed solution;
4) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) in the mixed solution obtained in the step 3) in an amount of 150g/L, continuously stirring at 600r/min for reaction for 4 hours, taking out, washing with deionized water until the washing solution is neutral, and drying at 60 ℃ to constant weight.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum, which comprises the reusable carrier and a composite microbial inoculum which is fixed in the carrier and consists of bacillus amyloliquefaciens, pseudomonas azotoformis, pseudomonas flavus, pseudomonas brucei, pseudomonas winkle, yeast and actinomycetes; the preparation method specifically comprises the following steps:
is compounded with 1.8 x 109cfu/mL Bacillus amyloliquefaciens, 1.8X 109cfu/mL Pseudomonas azotoformans, 2X 109cfu/mL Pseudomonas flacci, 2X 109cfu/mL Pseudomonas buchneri, 2X 109cfu/mL Pseudomonas winkle, 1.5X 109cfu/mL yeast and 1.5X 109A compound bacterium liquid consisting of cfu/mL actinomycetes; immersing 150g/L recyclable carrier into the bacterial liquid, fully mixing, standing for 36h, taking out, and drying for 30min at the temperature of below 40 ℃ to obtain the microbial inoculum.
Example 3: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) the basalt fiber (diameter 15 μm) is soaked in 10% acetone solution at a frequency of 32KHz and a density of 0.5W/cm2Ultrasonic cleaning for 5h under the condition, washing for 2 times by deionized water, soaking in 0.8mol/L hydrochloric acid solution at 35 deg.C for 45min, taking out, washing to neutral, drying at 45 deg.C for 2h, shearing into 12cm length, taking 15g basalt fiber, and fastening one end into umbrella shape;
2) heating ash after burning of populus diversifolia leaves at 580 ℃ for 2h, dispersing the ash in 0.1mol/L sodium hydroxide solution according to the mass ratio of 1.5:1 of the populus diversifolia ash to the sodium hydroxide, stirring and reacting for 3h at 50 ℃ with a cover of 300r/min, washing residues after filtering with distilled water, taking supernate after settling, adjusting the pH to be neutral, filtering with a 1500-mesh sieve, washing with deionized water, and drying for 48h at 50 ℃ to obtain the populus diversifolia white carbon black;
3) preparing 2g/L dopamine solution, adding 40mg/L white carbon black obtained in the step 2), stirring for 2h at 300r/min, and adding 30KHz of frequency and 0.3W/cm of density2Obtaining dopamine suspension by ultrasonic treatment; adding a Tris buffer solution with the volume of 20% of the dopamine suspension and the pH value of 7.2 into the dopamine suspension, adjusting the pH value to 8.0 by using a 0.5mol/L hydrochloric acid solution and/or a 0.5mol/L sodium hydroxide solution, and stirring at 450r/min for 45min to obtain a mixed solution;
4) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) in the mixed solution obtained in the step 3) in an amount of 120g/L, continuously stirring at 450r/min for reacting for 4h, taking out, washing with deionized water until the washing solution is neutral, and drying at the temperature of 55 ℃ to constant weight.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum, which comprises the reusable carrier and a composite microbial inoculum which is fixed in the carrier and consists of bacillus amyloliquefaciens, pseudomonas azotoformis, pseudomonas flavus, pseudomonas brucei, pseudomonas winkle, yeast and actinomycetes; the preparation method specifically comprises the following steps:
is compounded by 2 multiplied by 109cfu/mL Bacillus amyloliquefaciens, 2X 109cfu/mL Pseudomonas azotoformans, 2X 109cfu/mL Pseudomonas flaccid, 2X 109cfu/mL Pseudomonas buchneri, 2X 109cfu/mL Pseudomonas wrinkle, 2X 109cfu/mL Yeast and 2X 109A compound bacterium liquid consisting of cfu/mL actinomycetes; immersing 120g/L recyclable carrier into the bacterial liquid, fully mixing, standing for 30h, taking out, and drying for 30min at 38 ℃.
Example 4: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) soaking basalt fiber (diameter 15 μm) in 10% acetone solution at frequency of 32KHz and density of 0.5W/cm2Ultrasonic cleaning for 5h under the condition, washing with deionized water for 2 times, drying at 45 deg.C for 2h, shearing into length of 12cm, taking 15g basalt fiber, and fastening one end into umbrella shape;
2) same as step 2) of example 3;
3) same as step 3 of example 3);
4) same as step 4 of example 3).
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 5: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) heating ash after rice hull burning at 580 ℃ for 2h, dispersing the ash in 0.1mol/L sodium hydroxide solution according to the mass ratio of 1.5:1 of poplar ash to sodium hydroxide, stirring and reacting for 3h at 50 ℃, filtering, taking residues, washing with distilled water, settling, taking supernate, adjusting acid and alkali to be neutral, filtering with a 1500-mesh sieve, washing with deionized water, and drying at 50 ℃ for 48h to obtain rice hull white carbon black;
3) same as step 3 of example 3);
4) same as step 4 of example 3).
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 6: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) preparing 2g/L dopamine solution, adding 40mg/L ash from burning Populus euphratica leaves, stirring at 300r/min for 2 hr, and adding 30KHz frequency and 0.3W/cm density2Obtaining dopamine suspension by ultrasonic treatment; adding a Tris buffer solution with the volume of 20% of the dopamine suspension and the pH value of 7.2 into the dopamine suspension, adjusting the pH value to 8.0 by using a 0.5mol/L hydrochloric acid solution and/or a 0.5mol/L sodium hydroxide solution, and stirring at 450r/min for 45min to obtain a mixed solution;
3) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) in the mixed solution obtained in the step 2) in an amount of 120g/L, continuously stirring at 450r/min for reacting for 4h, taking out, washing with deionized water until the washing solution is neutral, and drying at the temperature of 55 ℃ to constant weight.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 7: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) preparing 2g/L dopamine solution, adding a Tris buffer solution with the volume of 20% of the dopamine suspension and the pH value of 7.2 into the dopamine solution, adjusting the pH value to 8.0 by using 0.5mol/L hydrochloric acid solution and/or 0.5mol/L sodium hydroxide solution, and stirring for 45min at 450r/min to obtain mixed solution;
3) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) in the mixed solution obtained in the step 2) in an amount of 120g/L, continuously stirring at 450r/min for reacting for 4h, taking out, washing with deionized water until the washing solution is neutral, and drying at the temperature of 55 ℃ to constant weight.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 8: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) same as step 2) of example 3;
3) preparing the aqueous suspension of the white carbon black obtained in the step 2) according to 40mg/L, stirring for 2h at 300r/min, and adding 30KHz of frequency and 0.3W/cm of density2Obtaining a suspension by ultrasonic processing; adding a Tris buffer solution with the volume of 20% of the suspension and the pH value of 7.2 into the suspension, adjusting the pH value to 8.0 by using a 0.5mol/L hydrochloric acid solution and/or a 0.5mol/L sodium hydroxide solution, and stirring for 45min at the speed of 450r/min to obtain a mixed solution;
4) same as step 4 of example 3).
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 9: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise: soaking basalt fiber (diameter 15 μm) in 10% acetone solution at frequency of 32KHz and density of 0.5W/cm2Ultrasonic cleaning for 5h under the condition, washing with deionized water for 2 times, and dissolving in 0.8mol/L hydrochloric acidSoaking in the solution at 35 deg.C for 45min, washing to neutral, drying at 45 deg.C for 2 hr, shearing into 12cm length, taking 15g basalt fiber, and fastening one end into umbrella shape.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 10: a perishable garbage rapid degradation microbial inoculum:
this example first provides a recyclable carrier prepared in the same manner as in example 3.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum, which comprises the reusable carrier and a composite microbial inoculum which is fixed in the carrier and consists of bacillus amyloliquefaciens, pseudomonas azotoformis, saccharomycetes and actinomycetes; the preparation method specifically comprises the following steps:
is compounded with 6 x 109cfu/mL Bacillus amyloliquefaciens, 6X 109cfu/mL Pseudomonas azotoformans, 6X 109cfu/mL Yeast and 6X 109A compound bacterium liquid consisting of cfu/mL actinomycetes; immersing 120g/L recyclable carrier into the bacterial liquid, fully mixing, standing for 30h, taking out, and drying for 30min at 38 ℃.
Experimental example 1: and (3) detecting the adsorption rate:
respectively diluting the bacterial liquid adsorbed by the carrier in the embodiments 1-10 into an original volume, counting the residual bacterial concentration by a dilution method, and calculating the adsorption rate of the carrier obtained in different embodiments by combining the original bacterial concentration:
the results of counting the adsorption rates of the complex microbial agents of examples 1 to 10 are shown in table 1.
TABLE 1 adsorption Rate
| Examples | Original bacterium concentration/cfu. mL-1 | Residual bacteria concentration/cfu. mL-1 | Adsorption rate/%) |
| 1 | 1.50×1010 | 1.32×109 | 91.20 |
| 2 | 1.26×1010 | 1.20×109 | 90.48 |
| 3 | 1.40×1010 | 1.06×109 | 92.43 |
| 4 | 1.40×1010 | 2.56×109 | 81.71 |
| 5 | 1.40×1010 | 3.28×109 | 76.57 |
| 6 | 1.40×1010 | 5.59×109 | 60.07 |
| 7 | 1.40×1010 | 6.00×109 | 57.14 |
| 8 | 1.40×1010 | 4.06×109 | 71.00 |
| 9 | 1.40×1010 | 6.68×109 | 52.29 |
| 10 | 1.40×1010 | 1.01×109 | 92.79 |
As can be seen from Table 1, the carriers in the preferred embodiments 1-3 of the present application all have an adsorption rate not lower than 90%, can enrich microorganisms to a large extent, improve the content of microorganisms in unit microbial inoculum, and are beneficial to the rapid degradation of perishable garbage. It can also be seen from table 1 that the adsorption rate of basalt fibers is significantly reduced in example 4 because acid etching is not performed on the basalt fibers, and the possible reason is that the fiber surface roughness is insufficient, so that active groups and carbon black cannot be grafted well during subsequent modification, while it can be seen from example 5 that the rice hulls are used for replacing populus euphratica to better adsorb microorganisms, it can be seen from example 6 that carbon black is extracted from populus euphratica ash to be used for modifying the adsorption effect of the basalt fibers on a lifting carrier, and it can be seen from examples 7 to 9 that the effect of adsorbing microorganisms cannot be obtained by directly using the desized and acid etched basalt fibers as a carrier without using the carbon black or dopamine.
Experimental example 2: and (3) detecting the degradation effect of the perishable garbage:
as shown in fig. 2, a perishable garbage degradation device is provided, which comprises a degradation bin 1 with a stirring rod 2 inside, wherein the stirring rod 2 can be driven by a stirring motor 4 arranged outside the degradation bin 1, a feeding port 3 for feeding perishable garbage is arranged at the top of the degradation bin 1, and a discharge port 5 for discharging perishable garbage degradation products is arranged at the bottom 4 of the degradation bin; respectively and sequentially detachably fixing the perishable garbage rapid degradation microbial inoculum 6 obtained in the embodiment 1-10 on the stirring rod according to the amount of 1.5kg/t/12 h; perishable garbage is picked to obtain nondegradable substances such as metal, fabrics, plastics, glass, rubber, stones and the like, the perishable garbage used in the application is the same batch, mainly comprises melon peel, rice, flour products, meat, vegetable leaves, fish, broken bones and the like, the moisture content is 75.4%, the wet content of organic matters is 74.3 +/-1.8%, the materials are crushed to be sieved by a 10-mesh sieve and are placed in a degradation bin 1, a stirring motor 4 is started to drive a stirring rod to rotate at 60r/min, and aerobic degradation is carried out for 36 hours at the room temperature of 20 +/-2 ℃; after the degradation is finished, the degradation products are discharged through a discharge opening 5, and the slag and the liquid are separated and are separately treated.
The weight of the perishable garbage before the microbial inoculum is added and the weight of the degraded slag of the perishable garbage after degradation are combined, the decrement rate of the perishable garbage rapid degradation microbial inoculum of each perishable garbage in the embodiments 1-10 to the perishable garbage is counted, and the counting result is shown in figure 3. As can be seen from fig. 3, in the preferred embodiment of the present application, each of the degrading bacteria in examples 1 to 3 can rapidly degrade perishable waste, the reduction rate in 36h is not lower than 90%, and in examples 4 to 9, the adsorption rate is reduced correspondingly due to the carrier structure, and the microorganism enrichment degree is insufficient, so that the reduction rates are reduced in different degrees, and in example 10, the perishable waste cannot be efficiently and rapidly degraded due to the lack of various microorganisms.
Experimental example 3: and (3) detecting the regeneration adsorption rate:
after the experimental example 2, the perishable garbage fast degradation microbial inoculum is taken out, washed clean by deionized water and dried, and then adsorbed to microorganisms (the compound bacterial liquid described in the example 3 is used during regeneration), and regenerated again after secondary utilization, and the adsorption rates of the microbial inoculum obtained in the examples 1-9 are respectively counted, as shown in table 2.
TABLE 2 regeneration adsorption Rate
As can be seen from the data in Table 2, the carrier obtained in the preferred embodiment 1-3 has the adsorption rate not lower than 85% in the second application and still has the adsorption rate not lower than 70% in the third utilization, so that the preparation cost and the use cost of the degrading microbial inoculum are greatly reduced, and the economic expenditure of the degradation treatment of perishable garbage is further reduced; as can be seen from the adsorption rates of the carriers obtained in examples 3 to 9 in the second and third uses, the reuse rate of the carrier is reduced because the active groups and particles on the surface of basalt fibers are not sufficiently modified, such as the fibers are not subjected to acid etching, carbon black is prepared by substituting rice hulls for populus diversifolia, carbon black is substituted by populus diversifolia ash, and the fibers are not modified by using white carbon black or dopamine; therefore, the carrier preparation process is optimized, so that the carrier preparation process has excellent adsorption rate, and can be used for preparing the degradation microbial inoculum again after the perishable garbage is degraded, and the preparation cost of the degradation microbial inoculum is greatly reduced.
Example 11: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) preparing a mixed solution containing 0.01mol/L zinc chloride and 0.015mol/L zirconium hypochlorite, soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) into the mixed solution in an amount of 100g/L, dropwise adding 2mol/L sodium hydroxide solution until the pH of the mixed solution is 9.5, heating to 70 ℃, stirring for reaction for 1 hour at 60r/min, taking out the basalt fiber cluster, washing with deionized water until the washing solution is neutral, and drying at 55 ℃ to obtain the zinc-zirconium modified basalt fiber;
3) same as step 2) of example 3;
4) same as step 3 of example 3);
5) soaking the umbrella-shaped modified basalt fiber obtained in the step 2) in the mixed solution obtained in the step 4) in an amount of 120g/L, continuously stirring at 450r/min for reaction for 4 hours, taking out, washing with deionized water until the washing solution is neutral, and drying at 55 ℃ to constant weight.
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 12: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) preparing an aqueous solution containing 0.01mol/L zinc chloride, soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) into the solution in an amount of 100g/L, dropwise adding 2mol/L sodium hydroxide solution until the pH value of the mixed solution is 9.5, heating to 70 ℃, stirring for reaction for 1h at 60r/min, taking out the basalt fiber cluster, washing with deionized water until the cleaning solution is neutral, and drying at 55 ℃ to obtain zinc modified basalt fiber;
3) same as step 2) of example 3;
4) same as step 3 of example 3);
5) same as step 5 of example 11).
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Example 13: a perishable garbage rapid degradation microbial inoculum:
the embodiment firstly provides a recyclable carrier, and the preparation steps specifically comprise:
1) same as step 1) of example 3;
2) preparing an aqueous solution containing 0.015mol/L zirconium hypochlorite, soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) into the solution in an amount of 100g/L, dropwise adding 2mol/L sodium hydroxide solution until the pH value of the mixed solution is 9.5, heating to 70 ℃, stirring for reaction for 1h at 60r/min, taking out the basalt fiber cluster, washing with deionized water until the cleaning solution is neutral, and drying at 55 ℃ to obtain the zirconium modified basalt fiber;
3) same as step 2) of example 3;
4) same as step 3 of example 3);
5) same as step 5 of example 11).
The embodiment also provides a perishable garbage rapid degradation microbial inoculum which is specifically prepared by the same corresponding method as the embodiment 3 based on the recyclable carrier provided by the embodiment.
Experimental example 4: and (3) detecting the degradation of the perishable garbage:
according to the scheme of the experimental example 2, the degradation effect of the perishable garbage rapid degradation microbial inoculum obtained in the example 3 and the examples 11-13 on perishable garbage is sequentially verified by only changing the room temperature to be 10 +/-2 ℃, and the statistical results are shown in the table 3.
TABLE 3 reduction rate
| Examples | Reduction rate/%) |
| 3 | 78.6 |
| 11 | 85.9 |
| 12 | 79.3 |
| 13 | 80.2 |
As can be seen from table 3, the basalt fiber is firstly subjected to zinc-zirconium modification and then grafted with dopamine and white carbon black, so that the degradation microbial inoculum still has an excellent degradation effect at a low temperature, and the reduction rate of the degradation microbial inoculum to the perishable garbage is not less than 85%.
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.
While the invention has been described in detail and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope thereof.
While the above detailed description has shown, described, and pointed out novel features as applied to various embodiments, it will be understood that various omissions, substitutions, and changes in the form and details of the device or method illustrated may be made without departing from the spirit of the disclosure. In addition, the various features and methods described above may be used independently of one another, or may be combined in various ways. All possible combinations and sub-combinations are intended to fall within the scope of the present disclosure. Many of the embodiments described above include similar components, and thus, these similar components are interchangeable in different embodiments. While the invention has been disclosed in the context of certain embodiments and examples, it will be understood by those skilled in the art that the invention extends beyond the specifically disclosed embodiments to other alternative embodiments and/or uses and obvious modifications and equivalents thereof. Accordingly, the invention is not intended to be limited by the specific disclosure of preferred embodiments herein.
The invention is not the best known technology.
Claims (9)
1. A preparation method of a recyclable carrier of a perishable garbage rapid degradation microbial inoculum is characterized by comprising the following steps:
1) soaking the basalt fibers in an acetone solution for ultrasonic cleaning for at least 4 hours, washing with deionized water for 2-4 times, placing in a hydrochloric acid solution, soaking at 35 ℃ for at least 30min, taking out, washing to be neutral, drying, shearing to be 10-15 cm in length, taking 10-15 g of basalt fibers, and fastening one end of each basalt fiber to be umbrella-shaped;
2) preparing 1-2 g/L dopamine solution, then adding 30-50 mg/L populus euphratica white carbon black, and ultrasonically stirring for at least 1h to obtain dopamine suspension; adding the Tris buffer solution into the dopamine suspension, adjusting the pH value to 7.8-8.0, and stirring for at least 30min to obtain a mixed solution;
3) soaking the umbrella-shaped basalt fiber cluster obtained in the step 1) in the mixed solution obtained in the step 2), continuously stirring and reacting for at least 4 hours, taking out, washing with deionized water until the washing solution is neutral, and drying to obtain the basalt fiber cluster;
the populus diversifolia white carbon black is prepared by the following method: heating ash after burning of populus diversifolia branches and/or leaves at 550-600 ℃ for 2h, dispersing the ash in a sodium hydroxide solution according to the mass ratio of 1.3-1.5: 1 of the populus diversifolia ash to the sodium hydroxide, stirring and reacting at 45-60 ℃ for at least 2h by adding a cover at 120-600 r/min, filtering, washing residues with distilled water, settling, taking supernate, adjusting acid and alkali to be neutral, filtering with a 1500-mesh sieve, washing with deionized water, and drying at 40-60 ℃ for 48h to obtain the product.
2. The method of claim 1, wherein:
the diameter of the basalt fiber is 12-15 mu m; and/or
The mass fraction of the acetone solution is 5-20%.
3. The perishable waste rapid degradation microbial inoculum prepared by the method of claim 1 or 2 can be recycled.
4. A perishable waste rapid degradation microbial inoculum based on the carrier of claim 3, characterized by comprising:
a perishable waste rapidly degrading microbial inoculum reusable carrier as claimed in claim 3, and
the composite microbial inoculum which is fixed in the carrier and consists of bacillus amyloliquefaciens, pseudomonas azotoformans, pseudomonas yellowish, pseudomonas brucei, pseudomonas winkle, saccharomycetes and actinomycetes.
5. A perishable waste rapid degradation microbial inoculum according to claim 4, wherein the preparation method comprises: the content of the compound total strains is 1-2 multiplied by 1010cfu/mL of composite bacterial liquid;
immersing 100-150 g/L of umbelliform basalt fiber carrier into the bacterial liquid, fully mixing, standing for 24-36 h, taking out, and drying for 30min at the temperature of below 40 ℃ to obtain the composite bacterial strain.
6. A perishable waste rapid degrading microbial inoculum according to claim 4 or 5, wherein: the content difference between strains in the composite bacteria is not more than 50%.
7. A method for rapidly degrading perishable garbage, which is characterized by comprising the following steps:
1) providing a degradation bin with a stirring rod inside, wherein the stirring rod can be driven by a stirring motor arranged outside the degradation bin, the top of the degradation bin is provided with a feeding port capable of feeding perishable garbage, and the bottom of the degradation bin is provided with a discharge port capable of discharging perishable garbage degradation products;
2) detachably fixing a perishable garbage rapid degrading microbial inoculum according to any one of claims 4-6 to the stirring rod;
3) after non-degradable substances are picked out from the perishable garbage, crushing the perishable garbage to pass through a 10-mesh sieve, placing the sieved material in a degradation bin, starting a stirring motor to drive a stirring rod to rotate at 30-120 r/min, and carrying out aerobic degradation for 24-36 hours; after the degradation is finished, the degradation product is discharged through a discharge hole, and the slag and the liquid are separated and are further treated.
8. The method of claim 7, wherein:
the perishable garbage rapid degradation microbial inoculum is arranged and replaced according to the amount of 1-2 kg/t/12 h; and/or
The aerobic degradation temperature is 15-40 ℃, and the relative humidity is 80-90%; and/or ventilating 8-10 times/h during aerobic degradation.
9. Use of the degradation method of claim 7 or 8 for the rapid degradation of perishable waste.
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