CN113151086B - Siamese bacillus, microbial inoculum, extract and application thereof - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a Siamese bacillus, a microbial inoculum, an extract and application thereof, relating to the technical field of biological microbial inoculum, wherein the Siamese bacillus JZ1-4-10 is preserved in Guangdong province microbial strain preservation center in 10, 15 months in 2020, and the preservation number is as follows: GDMCC No:61236; the extract is obtained by culturing the Siamese bacillus to obtain fermentation liquor and then extracting the fermentation liquor by using an organic solvent. The strain can effectively inhibit fusarium causing potato dry rot, especially for Yuqing 9A-2-6 (C)Fusarium solani) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum) The inhibition effect is obvious.
Description
Technical Field
The invention relates to the technical field of biological inoculants, in particular to a Siamese bacillus, an inoculant, an extract and application thereof.
Background
The potato is the fourth most important grain crop in the world, and is easily attacked by a plurality of diseases such as potato late blight, potato black nevus, potato blight, potato dry rot, potato early blight, potato cancerization, potato silver rot, potato verticillium wilt, potato powdery scab and the like in the storage process. Among them, the potato dry rot is one of the major fungal diseases in the storage period of potatoes, and is composed of a plurality of fusarium (F.) (Fusariumspp.) causes, in particular, the fungus cyan 9A-2-6 (in FusariumFusarium tricinctum) 9A-5-7 (B)Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum). According to statistics, the yield loss caused by the dry rot of the potatoes is 6% -25% every year, and the highest yield can reach 60%. At present, chemical control is mainly used for the disease, commonly used medicaments comprise difenoconazole, azoxystrobin, cymoxanil, manganese zinc, tebuconazole and the like, but strains exist for long-term chemical controlThe drug resistance is enhanced, the environment is polluted, the pesticide residue is remained and the like.
Disclosure of Invention
In order to solve the above problems, the present invention provides a Bacillus siamensis, wherein the name of the Bacillus siamensis (Siamese Bacillus) (II)Bacillus siamensis）JZ1-4-10。
The Siamese bacillus provided by the invention is preserved in Guangdong province microorganism strain preservation center in 10, 15 th of 2020, and the preservation number is as follows: GDMCC No:61236.
the Siamese bacillus sieve provided by the invention is selected from highland barley distilled spirit grains.
In order to solve the problems, the invention also provides a microbial inoculum which comprises the Siamese bacillus JZ1-4-10.
The preparation method of the microbial inoculum comprises the steps of culturing the Siamese bacillus provided by the invention and adding corresponding auxiliary materials to prepare the microbial inoculum.
In order to solve the problems, the invention also provides an extract of the Siamese bacillus fermentation liquor, wherein the Siamese bacillus fermentation liquor is obtained by culturing the Siamese bacillus fermentation liquor and then extracting the Siamese bacillus fermentation liquor by using an organic solvent.
The method specifically comprises the following steps: inoculating Siamese bacillus JZ1-4-10 strain into ATCC213 improved liquid culture medium to culture for 5-7 d, harvesting fermentation liquor, and then filtering to remove thallus to obtain thallus-removed fermentation liquor.
Placing 600mL of fermentation liquor in a 3000mL separating funnel, sequentially extracting the fermentation liquor with chloroform, ethyl acetate and n-butanol which are equal in volume, and performing rotary evaporation on the extract to obtain an extract.
The Siamese bacillus, the microbial inoculum containing the Siamese bacillus and the Siamese bacillus fermentation liquor extract can be used for inhibiting fusarium, and the fusarium comprises but is not limited to cyan 9A-2-6 (C)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The use of (1).
In order to solve the problems, the invention also provides the Siamese bacillus, a microbial inoculum containing the Siamese bacillus and the Siamese bacillus fermentation liquor extract for preventing and controlling the cyan 9A-2-6 (Siamese bacillus extract)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The application in the dry rot caused by the disease.
Cyan 9A-2-6 (Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The induced dry rot includes, but is not limited to, potato dry rot.
Further, the dry rot is potato dry rot.
Biological preservation description:
classification nomenclature of biological materials: bacillus siamensis: (Bacillus siamensis）；
Strain number of biological material: JZ1-4-10;
name of the depository of biological material: guangdong province microbial strain preservation center;
the preservation unit of the biological material is abbreviated as: GDMCC;
storage unit address of the stored material: building No. 59, building No. 5 of the first-furious Zhonglu 100 yard in Guangzhou city;
preservation date of biological material: 10 and 15 days in 2020;
accession number to the collection of biological materials: GDMCC No:61236.
the invention has the beneficial effects that:
the Siamese bacillus, the microbial inoculum containing the Siamese bacillus and the Siamese bacillus fermentation liquor extract provided by the invention can effectively inhibit fusarium causing the dry rot of potatoes, particularly to green 9A-2-6 (Siamese bacillus extract)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The inhibition effect is remarkable, and the inhibition rate of Siamese bacillus on the blue green 9A-2-6 can reach 74.12 percent; siam sporulation rodThe inhibition rates of the ethyl acetate extract and the n-butanol extract of the strain fermentation liquor on fusarium wilt blue 9A-2-6 are respectively up to 77.34% and 78.00%, the inhibition rates of the ethyl acetate extract and the n-butanol extract on pathogen blue 9A-4-13 are up to 66.50% and 58.00%, the inhibition rate of the n-butanol extract on blue 9A-5-7 is 72.50%, and the inhibition rate of the ethyl acetate extract on blue 9A-4-13 is 66.50%.
In addition to the above-described objects, features and advantages, the present invention has other objects, features and advantages. The present invention will be described in further detail below with reference to the drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.
FIG. 1 is a phylogenetic tree of Bacillus siamensis JZ1-4-10 according to an embodiment of the present invention;
FIG. 2 is a graph of the inhibitory effect of Siamese bacillus JZ1-4-10 on potato pathogenic fungus cyan 9A-2-6 in the embodiment of the invention;
FIG. 3 is a graph of the inhibitory effect of Siamese bacillus JZ1-4-10 on potato pathogenic fungus cyan 9A-5-7 in the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The strains used in the embodiments of the present invention include: pathogenic fungus green 9A-2-6 (for potato dry rot disease)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (B)Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum) The pathogenic fungi are separated from the potato tuber 9 disease sample; a Siamese bacillus JZ1-4-10 strain is separated from highland barley white spirit fermented grains, the strains are purified and then are stored in a microorganism laboratory of institute of biotechnology, institute of agriculture and forestry, qinghai province, and are stored in a refrigerator at 4 DEG C。
The culture medium adopted by the embodiment of the invention comprises a potato glucose culture medium and a beef extract peptone culture medium.
The potato glucose culture medium comprises: 200g of potatoes, 15g of glucose, 15g of agar and 1000mL of distilled water.
The beef extract peptone culture medium comprises: 3g of beef extract, 10g of peptone, 5g of sodium chloride, 20g of agar and 1000mL of distilled water, and adjusting the pH value to 7.0-7.2.
The apparatus used included: the electronic balance is purchased from Mettler-Torledo instruments, inc., the high-pressure steam sterilization pot is purchased from Huayue Enterprise group, inc., the electric heating constant-temperature drying box is purchased from Shanghai Qixin scientific instruments, inc., the biochemical culture box is purchased from Shanghai Hengxin scientific instruments, inc., and the full-temperature shaking bottle cabinet is purchased from Changzhou Jintan Jingda instruments, inc.
Example 1
The application of the activation of Siamese bacillus:
taking out the stored Siamese bacillus JZ1-4-10 strain separated from the highland barley white spirit fermented grains from a refrigerator at 4 ℃, inoculating the Siamese bacillus JZ on a beef extract peptone culture medium, and placing the Siamese bacillus JZ in a constant-temperature culture box at 37 ℃ for culturing for 48 hours for later use.
The application of the identification of Siamese bacillus:
according to Bergey's Manual of bacteria identification (ninth edition) and Manual of identification of common bacteria systems, the strain JZ1-4-10 was subjected to glucose oxidative fermentation, citrate utilization, catalase, sugar and alcohol fermentation, litmus milk test, gelatin liquefaction, methyl red (M.R), nitrate reduction, V-P determination, O-nitrophenyl-beta-D galactopyranoside (ONPG) determination, and the results are shown in Table 1.
TABLE 1 physiological and biochemical characteristics of Siamese bacillus JZ1-4-10
As shown in Table 1, the strain JZ1-4-10 can liquefy gelatin, solidify litmus milk, utilize sucrose, maltose and citric acid, and utilize aerobic or anaerobic molecules to participate in the decomposition of glucose, and can produce ONPG, and the catalase test and the V-P test are negative. The strain JZ1-4-10 can not utilize mannitol, and has negative methyl red test and positive nitrate reduction test.
Molecular biology identification of Siamese bacillus:
the method adopts a column type bacteria DNA extraction kit of Shanghai biological engineering (Shanghai) corporation to perform program extraction on DNA of Siamese bacillus JZ1-4-10. The universal bacteria 16S rDNA primers F27 (5-. The PCR amplification system is as follows: 10 Xbuffer 2.5. Mu.L, dNTP (10 mmol. Multidot.L) -1 ) mu.L of 2. Mu.L of template DNA, 1. Mu.L of each primer, 0.2. Mu.L of Taq DNA polymerase and distilled water to make up to 25. Mu.L.
The PCR reaction conditions were as follows: denaturation at 94 deg.C for 5min; denaturation at 94 ℃ for 40s, annealing at 55 ℃ for 45s, extension at 72 ℃ for 80s, and 35 cycles; further extension was carried out at 72 ℃ for 10min. After the reaction is finished, 5 mu L of PCR product is taken for agarose gel electrophoresis detection, the recovered product is sent to the Shanghai biological engineering company for sequencing, and the nucleotide sequence of the Siamese bacillus is obtained as SEQ ID No:1 is shown.
The obtained 16S rDNA sequence is compared with the known 16S rDNA sequence in a database on a website https:// www.ezbio _ bound.net/, a sequence with homology of more than 95 percent with the 16S rDNA sequence of Siamese bacillus JZ1-4-10 is downloaded, and a phylogenetic tree is constructed according to a Neighbor-Joining method by using Mega (7.0) software, as shown in figure 1. The result shows that the strain JZ1-4-10 and Siamese Bacillus (Bacillus siamensis) cluster the same branch, the genetic relationship is recent, the similarity is as high as 99.58%, and finally the strain JZ1-4-10 is identified as Siamese Bacillus (Siamese Bacillus) by combining the physiological and biochemical characteristics of the Siamese Bacillus JZ1-4-10 and the sequence analysis result of 16S rDNABacillus siamensis）。
Example 2
Screening of active strains and evaluation of stability:
culture dish with diameter of 9cm by adopting flat plate opposing methodOne side of the edge 2cm is connected with Siamese bacillus strain separated from highland barley white spirit grains, and the other opposite side is respectively connected with pathogenic fungus green 9A-2-6 (5 mm in diameter)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum) The treatment was repeated 3 times for each pathogenic fungus, and the control was made of the treatment with only the pathogenic fungus. Culturing in 28 deg.C biochemical incubator for 7 days, observing whether there is bacteria inhibition zone between strain separated from fermented grains of highland barley Chinese liquor and pathogenic fungi, measuring colony diameter, and calculating bacteria inhibition rate, wherein the calculation formula is as follows:
inhibition (%) = [ (control colony diameter-treated colony diameter)/control colony diameter ] × 100
The results are shown in table 2 and fig. 2 and 3.
TABLE 2 Activity of Siamese bacillus JZ1-4-10 against Fusarium
In order to determine the stability of the active strains, the stability evaluation is carried out on the strains with the bacteriostatic activity obtained by primary screening, and the method is the same as the above method. Respectively with the Siamese bacillus of this application and fusarium confrontation cultivation 7d and 14d after, measure antibacterial bandwidth width, antibacterial bandwidth width reduces rate (VF) when calculating 14d, compares Siamese bacillus to various fusarium's suppression stability.
The stability evaluation criteria were: "- -": VF is more than or equal to 70 percent and is unstable; "-": VF more than or equal to 50 percent and less than 70 percent, is relatively unstable; "+": VF being more than or equal to 20 percent and less than 50 percent is more stable; "++": VF is less than 20 percent and is stable. The calculation formula is as follows:
VF (%) = [ (7 d bacteriostasis bandwidth value-14 d bacteriostasis bandwidth value)/7 d bacteriostasis bandwidth value ] × 100
The results are shown in table 3:
TABLE 3 stability of Siamese Bacillus JZ1-4-10 against pathogenic bacteria
As can be seen from Table 3, the Siamese bacillus JZ1-4-10 strain of the application has stable bacteriostatic action on fusarium solani 9A-2-6, green 9A-5-7 and green 9A-5-10, and the stability is '+'; has stable bacteriostatic action on the blue 9A-4-13, and the stability is plus.
Determination of prevention and treatment effect of Siamese bacillus JZ1-4-10 on potato dry rot
Firstly inoculating activated Siamese bacillus JZ1-4-10 in ATCC213 modified liquid culture medium, and inoculating at 37 ℃ for 200 r.min -1 Shake-culturing in shake flask for 2d, and harvesting the fermentation liquid. The concentration of JZ1-4-10 strain fermentation broth was adjusted to 1.0X 10 using a hemocytometer 8 cfu·mL -1 (ii) a Inoculating 4 kinds of Fusarium causing potato dry rot in PDA liquid culture medium, respectively, and culturing at 28 deg.C and 200 r min -1 Shake-culturing in shake flask for 2d, and harvesting the fermentation liquid. The spore concentration of 4 kinds of fusarium fermentation liquor is respectively adjusted to 1.0 multiplied by 10 by a blood counting chamber 7 each.mL -1 .2 orifices 3mm deep were then punched in the sterilized potatoes with a 5mm diameter punch. And each orifice is firstly accessed with 40 mu L of fusarium fermentation liquor, and then is accessed with 40 mu L of Siamese bacillus fermentation liquor to serve as an experimental group. Wrapping the inoculated potatoes with a sealing film, putting the wrapped potatoes into a self-sealing bag, and culturing for 14d in a constant-temperature incubator at the temperature of 28 ℃ and the relative humidity of about 75%. Treating fusarium inoculation fermentation liquid and equivalent sterile water as positive control; the treatment with the same amount of sterile water and the Siamese bacillus fermentation liquor is used as a negative control, and the treatment with only the same amount of sterile water is used as a blank control. One potato was inoculated per treatment and repeated 3 times.
After the potato tubers are cultured for 14 days, the fruit rot rate, the depth of the disease spots and the inhibition rate of the potato tubers treated by the fermentation liquor and the extract solution are respectively measured, and the calculation formula of the inhibition rate is as follows.
Inhibition (%) = LP-LA/LP × 100%.
Wherein LP is the potato tuber quality of the blank control group-the potato tuber quality of the positive control group, and LA is the potato tuber quality of the negative control group-the potato tuber quality of the experimental group. The results are shown in Table 4 below,
TABLE 4 Siamese Bacillus JZ1-4-10 inhibiting effect on Fusarium
As can be seen from the table, the inhibitory activities of the JZ1-4-10 fermentation broth on 4 fusarium species are respectively cyan 9A-2-6, cyan 9A-5-7, cyan 9A-4-13 and cyan 9A-5-10 from high to low. Wherein the rate of bad fruits of the JZ1-4-10 fermentation liquor on fusarium wilt 9A-2-6 is 0.78%, the depth of disease spots is 0.69 cm, and the inhibition rate reaches 75.33%; the rate of bad fruits for green 9A-5-7 is 0.35%, the depth of lesion spots is 0.89 cm, and the inhibition rate reaches 66.15%; the rate of bad fruits for the green 9A-4-13 is 0.62 percent, the depth of the disease spot is 0.93 cm, and the inhibition rate is 60.85 percent; the rate of bad fruits for green 9A-5-10 is 0.49%, the depth of lesion spots is 1.25 cm, and the inhibition rate reaches 43.26%.
Example 3
Preparation of Siamese bacillus JZ1-4-10 fermentation liquor and extract thereof:
inoculating Siamese bacillus JZ1-4-10 strain into an ATCC213 modified liquid culture medium with the bottling volume of 400mL, and placing at 37 ℃ for 180r min -1 Carrying out shake culture in a constant-temperature shake flask cabinet for 5-7 d, harvesting the fermentation liquor, and then filtering the fermentation liquor under reduced pressure by using a Buchner funnel to remove thalli to obtain the thalli-removed fermentation liquor for later use.
Placing 600mL of the thallus-removed fermentation liquor into a 3000mL separating funnel, sequentially extracting the fermentation liquor by using chloroform, ethyl acetate and n-butanol which are equal in volume, and carrying out rotary evaporation on the extract to obtain an extract; preparing the extract into extract solutions with mass concentrations of 1.00, 5.00, 10.00 and 20.00 mg/mL < -1 > respectively by using sterile water, and performing suction filtration on the solutions through a 0.22 mu m microporous filter membrane for later use.
The same method as that for the fermentation broth in example 2 was used to verify the inhibitory effect of the fermentation broth extract on the pathogens of potato dry rot, and the results are shown in table 5.
TABLE 5 inhibitory Effect of Siamese Bacillus JZ1-4-10 extract on pathogenic bacteria of dried rot disease of Potato
As can be seen from Table 5, the n-butanol extract of the fermentation broth of JZ1-4-10 strain had the highest inhibitory activity against 4 species of Fusarium which causes potato dry rot, the ethyl acetate extract had the lowest activity, and the chloroform extract had the lowest activity. Wherein the content of n-butanol extract is 10 mg/mL -1 The inhibition rate of the fusarium wilt enzyme 9A-2-6 is as high as 70.25% under the test concentration; at 20 mg. ML -1 The inhibition rates of the fusarium graminearum 9A-2-6 and the fusarium graminearum 9A-5-7 are respectively as high as 78.00% and 72.5% under the test concentration; the inhibition rate of the fusarium wilt enzyme 9A-4-13 is 58.00%. The ethyl acetate extract content was 10 mg. Multidot.mL -1 Under the test concentration of (1), the inhibition rate of the pathogenic bacteria cyan 9A-2-6 is 56.00 percent; at 20 mg. ML -1 The inhibition rates of the test solution on pathogenic bacteria blue 9A-2-6 and blue 9A-4-13 are respectively up to 77.34% and 66.50%.
10 mg·mL -1 And 20 mg. ML -1 The ethyl acetate extract and the n-butanol extract under all the tested concentrations have stronger inhibition effect on the pathogenic bacteria blue 9A-2-6, and the inhibition rate ranges from 56.00% to 78.00%. Only 20 mg. ML -1 The n-butanol extract has strong inhibition effect on pathogenic bacteria blue 9A-5-7, and the inhibition rate is as high as 72.50%. Only 20 mg. ML -1 The ethyl acetate and n-butanol extracts have strong inhibition effect on pathogenic bacteria cyan 9A-4-13, and the inhibition rate reaches 66.50% and 58.00%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Sequence listing
<110> academy of agriculture and forestry of Qinghai province
<120> Siamese bacillus, microbial inoculum, extract and application thereof
<130> 1
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1414
<212> DNA
<213> Siamese Bacillus (Bacillus siamensis)
<400> 1
gcagtcgagc ggacagatgg gagcttgctc cctgatgtta gcggcggacg ggtgagtaac 60
acgtgggtaa cctgcctgta agactgggat aactccggga aaccggggct aataccggat 120
ggttgtttga accgcatggt tcagacataa aaggtggctt cggctaccac ttacagatgg 180
acccgcggcg cattagctag ttggtgaggt aacggctcac caaggcgacg atgcgtagcc 240
gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg 300
cagcagtagg gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga 360
tgaaggtttt cggatcgtaa agctctgttg ttagggaaga acaagtgccg ttcaaatagg 420
gcggcacctt gacggtacct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 480
taatacgtag gtggcaagcg ttgtccggaa ttattgggcg taaagggctc gcaggcggtt 540
tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactggggaa 600
cttgagtgca gaagaggaga gtggaattcc acgtgtagcg gtgaaatgcg tagagatgtg 660
gaggaacacc agtggcgaag gcgactctct ggtctgtaac tgacgctgag gagcgaaagc 720
gtggggagcg aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctaag 780
tgttaggggg tttccgcccc ttagtgctgc agctaacgca ttaagcactc cgcctgggga 840
gtacggtcgc aagactgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 900
tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tctgacaatc 960
ctagagatag gacgtcccct tcgggggcag agtgacaggt ggtgcatggt tgtcgtcagc 1020
tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc 1080
agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg 1140
acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggacagaaca 1200
aagggcagcg aaaccgcgag gttaagccaa tcccacaaat ctgttctcag ttcggatcgc 1260
agtctgcaac tcgactgcgt gaagctggaa tcgctagtaa tcgcggatca gcatgccgcg 1320
gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1380
cgaagtcggt gaggtaacct ttatggagcc agcc 1414
Claims (4)
1. A Siamese bacillus is characterized in that the Siamese bacillus JZ1-4-10 is preserved in Guangdong province microorganism strain preservation center in 2020, 10 and 15 months, and the preservation number is as follows: GDMCC No:61236.
2. a microbial inoculant comprising bacillus siamensis JZ1-4-10 of claim 1.
3. A Siamese bacillus fermentation liquor extract is characterized in that the extract is obtained by culturing Siamese bacillus of claim 1 to obtain fermentation liquor and then extracting the fermentation liquor by using an organic solvent; the organic solvent is one of ethyl acetate and n-butyl alcohol; the culture medium used for culturing Siamese bacillus is an ATCC213 modified liquid culture medium.
4. Use of the bacillus siamensis JZ1-4-10 of claim 1, the microbial inoculum of claim 2 or the extract of claim 3 for preventing and treating dry rot of potato.
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| CN108034618A (en) * | 2018-01-24 | 2018-05-15 | 吉林省农业科学院 | Siam's Bacillus strain and its application |
| CN111690578A (en) * | 2020-07-31 | 2020-09-22 | 山东佐田氏生物科技有限公司 | Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof |
| CN111849844A (en) * | 2020-09-02 | 2020-10-30 | 青海省农林科学院 | Bacillus licheniformis A10201 and application thereof |
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| CN108034618A (en) * | 2018-01-24 | 2018-05-15 | 吉林省农业科学院 | Siam's Bacillus strain and its application |
| CN111876344A (en) * | 2020-06-29 | 2020-11-03 | 广西大学 | 24-membered ring macrolide high-yield strain and application thereof |
| CN111690578A (en) * | 2020-07-31 | 2020-09-22 | 山东佐田氏生物科技有限公司 | Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof |
| CN111849844A (en) * | 2020-09-02 | 2020-10-30 | 青海省农林科学院 | Bacillus licheniformis A10201 and application thereof |
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