CN113151086A - Siamese bacillus, microbial inoculum, extract and application thereof - Google Patents

Siamese bacillus, microbial inoculum, extract and application thereof Download PDF

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CN113151086A
CN113151086A CN202110444947.1A CN202110444947A CN113151086A CN 113151086 A CN113151086 A CN 113151086A CN 202110444947 A CN202110444947 A CN 202110444947A CN 113151086 A CN113151086 A CN 113151086A
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fusarium
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bacillus
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CN113151086B (en
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沈硕
李玮
王舰
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Qinghai Academy of Agricultural and Forestry Sciences
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract

The invention discloses a Siamese bacillus, a microbial inoculum, an extract and application thereof, relating to the technical field of biological microbial inoculants, wherein the Siamese bacillus JZ1-4-10 is preserved in Guangdong province microbial strain preservation center in 10, 15 months in 2020, and the preservation number is as follows: GDMCC No: 61236, respectively; the extract is obtained by culturing the Siamese bacillus to obtain fermentation liquor and then extracting the fermentation liquor by using an organic solvent. The strain can effectively inhibit fusarium causing potato dry rot, especially for green 9A-2-6 (C)Fusarium solani) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum) The inhibition effect is obvious.

Description

Siamese bacillus, microbial inoculum, extract and application thereof
Technical Field
The invention relates to the technical field of biological inoculants, in particular to a Siamese bacillus, an inoculant, an extract and application thereof.
Background
The potato is the fourth most important grain crop in the world, and is easily attacked by potato late blight, potato black nevus, potato blight and horse in the storage processPotato dry rot, potato early blight, potato cancerization, potato silver rot, potato verticillium wilt, potato powdery scab and other diseases. Among them, the potato dry rot is one of the major fungal diseases in the storage period of potatoes, and is composed of a plurality of fusarium (F.) (Fusariumspp.) causes, in particular, the fungus cyan 9A-2-6 (FusariumFusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum). According to statistics, the yield loss caused by the dry rot of the potatoes is 6-25% every year, and the maximum yield can reach 60%. At present, chemical prevention and control are mainly performed on the disease, commonly used medicaments comprise difenoconazole-azoxystrobin, Freon urea-manganese zinc, tebuconazole and the like, but the problems of enhanced drug resistance of strains, environmental pollution, pesticide residue and the like exist when the chemical medicaments are used for preventing and controlling the disease for a long time.
Disclosure of Invention
In order to solve the above problems, the present invention provides a Bacillus siamensis, wherein the name of the Bacillus siamensis (Siamese Bacillus) (II)Bacillus siamensis）JZ1-4-10。
The Siamese bacillus provided by the invention is preserved in Guangdong province microorganism strain preservation center in 10, 15 th of 2020, and the preservation number is as follows: GDMCC No: 61236.
the Siamese bacillus sieve provided by the invention is selected from highland barley distilled spirit grains.
In order to solve the problems, the invention also provides a microbial inoculum which comprises the Siamese bacillus JZ 1-4-10.
The preparation method of the microbial inoculum comprises the steps of culturing the Siamese bacillus provided by the invention and adding corresponding auxiliary materials to prepare the microbial inoculum.
In order to solve the problems, the invention also provides an extract of the Siamese bacillus fermentation liquor, wherein the Siamese bacillus fermentation liquor is obtained by culturing the Siamese bacillus fermentation liquor and then extracting the Siamese bacillus fermentation liquor by using an organic solvent.
The method specifically comprises the following steps: inoculating Siamese bacillus JZ1-4-10 strain into an ATCC213 improved liquid culture medium for culturing for 5-7 d, harvesting fermentation liquor, and then filtering to remove thallus to obtain thallus-removed fermentation liquor.
Placing 600mL of fermentation liquor in a 3000mL separating funnel, sequentially extracting the fermentation liquor with chloroform, ethyl acetate and n-butanol which are equal in volume, and performing rotary evaporation on the extract to obtain an extract.
The Siamese bacillus, the microbial inoculum containing the Siamese bacillus and the Siamese bacillus fermentation liquor extract can be used for inhibiting fusarium, and the fusarium comprises but is not limited to cyan 9A-2-6 (C)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The use of (1).
In order to solve the problems, the invention also provides the Siamese bacillus, a microbial inoculum containing the Siamese bacillus and the Siamese bacillus fermentation liquor extract for preventing and controlling the cyan 9A-2-6 (Siamese bacillus extract)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The application in the dry rot caused by the disease.
Cyan 9A-2-6 (Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The induced dry rot includes, but is not limited to, potato dry rot.
Further, the dry rot is potato dry rot.
Biological preservation description:
classification nomenclature of biological materials: bacillus siamensis: (Bacillus siamensis）；
Strain number of biological material: JZ 1-4-10;
deposit name of biological material: guangdong province microbial strain preservation center;
the preservation unit of the biological material is abbreviated as: GDMCC;
storage unit address of the stored material: building No. 59, building No. 5 of the first-furious Zhonglu 100 yard in Guangzhou city;
preservation date of biological material: 10 and 15 days in 2020;
accession number to the collection of biological materials: GDMCC No: 61236.
the invention has the beneficial effects that:
the Siamese bacillus, the microbial inoculum containing the Siamese bacillus and the Siamese bacillus fermentation liquor extract provided by the invention can effectively inhibit fusarium causing the dry rot of potatoes, particularly to green 9A-2-6 (Siamese bacillus extract)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The inhibition effect is remarkable, and the inhibition rate of Siamese bacillus on the green 9A-2-6 can reach 74.12%; the inhibition rates of the Siamese bacillus fermentation liquor ethyl acetate extract and the n-butyl alcohol extract on fusarium wilt 9A-2-6 are respectively up to 77.34% and 78.00%, the inhibition rates of the ethyl acetate extract and the n-butyl alcohol extract on pathogenic bacteria wilt 9A-4-13 are up to 66.50% and 58.00%, the inhibition rate of the n-butyl alcohol extract on wilt 9A-5-7 is 72.50%, and the inhibition rate of the ethyl acetate extract on wilt 9A-4-13 is 66.50%.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.
FIG. 1 is a phylogenetic tree of Bacillus siamensis JZ1-4-10 according to an embodiment of the present invention;
FIG. 2 is a graph of the inhibitory effect of Siamese Bacillus JZ1-4-10 on potato pathogenic fungus cyan 9A-2-6 in accordance with an embodiment of the present invention;
FIG. 3 is a graph showing the inhibitory effect of Bacillus siamensis JZ1-4-10 on potato pathogenic fungus cyan 9A-5-7 in accordance with an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The strains used in the embodiments of the present invention include: pathogenic fungus green 9A-2-6 (for potato dry rot disease)Fusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum) The pathogenic fungi are separated from the potato tuber 9 disease sample; siamese bacillus JZ1-4-10 strain is separated from highland barley white spirit fermented grains, and the strain is purified and then stored in a microbiological laboratory of the institute of biotechnology, institute of agriculture and forestry, Qinghai province, and stored in a refrigerator at 4 ℃.
The culture medium adopted by the embodiment of the invention comprises a potato glucose culture medium and a beef extract peptone culture medium.
The potato glucose culture medium comprises: 200g of potatoes, 15g of glucose, 15g of agar and 1000mL of distilled water.
The beef extract peptone culture medium comprises: 3g of beef extract, 10g of peptone, 5g of sodium chloride, 20g of agar and 1000mL of distilled water, and adjusting the pH value to 7.0-7.2.
The apparatus used included: the electronic balance is purchased from Mettler-Torledo instruments, Inc., the high-pressure steam sterilization pot is purchased from Huayue Enterprise group, Inc., the electric heating constant-temperature drying box is purchased from Shanghai Qixin scientific instruments, Inc., the biochemical culture box is purchased from Shanghai Hengxin scientific instruments, Inc., and the full-temperature shaking bottle cabinet is purchased from Changzhou Jintan Jingda instruments, Inc.
Example 1
The application of the activation of Siamese bacillus:
taking out the stored Siamese bacillus JZ1-4-10 strain separated from the highland barley white spirit fermented grains from a refrigerator at 4 ℃, inoculating the Siamese bacillus JZ1-4-10 strain on a beef extract peptone culture medium, and culturing for 48 hours in a constant-temperature culture box at 37 ℃ for later use.
The application of the identification of Siamese bacillus:
the results of tests such as glucose oxidative fermentation, citrate utilization, catalase, sugar and alcohol fermentation, litmus milk test, gelatin liquefaction, methyl red (M.R), nitrate reduction, V-P assay, and O-nitrobenzene-beta-D galactopyranoside (ONPG) assay were performed on strain JZ1-4-10 according to Bergey's Manual of bacteria identification (ninth edition) and Manual of general bacteria System identification (ORA), respectively, and are shown in Table 1.
TABLE 1 physiological and biochemical characteristics of Siamese bacillus JZ1-4-10
Figure DEST_PATH_IMAGE001
Note: "+" indicates positive, and "-" indicates negative
As shown in Table 1, JZ1-4-10 can liquefy gelatin, coagulate litmus milk, utilize sucrose, maltose and citric acid, and utilize aerobic or anaerobic molecules to decompose glucose to produce ONPG, which is negative in both catalase test and V-P test. The strain JZ1-4-10 can not utilize mannitol, and is negative in methyl red test and positive in nitrate reduction.
Molecular biology identification of Siamese bacillus:
the DNA of Siamese bacillus JZ1-4-10 is extracted by a column type bacterium DNA extraction kit of Shanghai biological engineering (Shanghai) corporation. The universal bacterial 16S rDNA primers F27 (5'-AGAGTTTGATCCTGGCTCA GG-3') and P1541 (5'-AAGGAGGTGGTGATCCAGCCG CA-3') were selected. The PCR amplification system is as follows: 10 Xbuffer 2.5. mu.L, dNTP (10 mmol. multidot.L)-1) mu.L of 2. mu.L of template DNA, 1. mu.L of each primer, 0.2. mu.L of Taq DNA polymerase and distilled water to make up to 25. mu.L.
The PCR reaction conditions were as follows: denaturation at 94 deg.C for 5 min; denaturation at 94 ℃ for 40s, annealing at 55 ℃ for 45s, and extension at 72 ℃ for 80s for 35 cycles; further extension was carried out at 72 ℃ for 10 min. After the reaction is finished, 5 mu L of PCR product is taken for agarose gel electrophoresis detection, the recovered product is sent to the Shanghai biological engineering company for sequencing, and the nucleotide sequence of the Siamese bacillus is obtained as SEQ ID No: 1 is shown.
The obtained 16S rDNA sequence is compared with the known 16S rD in the database on the website https:// www.ezbio clusterThe NA sequences were aligned and sequences with homology greater than 95% to the 16S rDNA sequence of Siamese Bacillus JZ1-4-10 were downloaded and phylogenetic trees were constructed using Mega (7.0) software according to the Neighbor-Joining method, as shown in FIG. 1. The result shows that the strain JZ1-4-10 and Siamese Bacillus (Bacillus siamensis) cluster the same branch, the genetic relationship is recent, the similarity is as high as 99.58%, and finally the strain JZ1-4-10 is identified as the Siamese Bacillus (Bacillus siamensis) by combining the physiological and biochemical characteristics of the Siamese Bacillus JZ1-4-10 and the sequence analysis result of 16S rDNABacillus siamensis）。
Example 2
Screening of active strains and evaluation of stability:
adopting a plate opposing method to connect a Siamese bacillus strain separated from highland barley white spirit grains at one side of 2cm position of the edge of a culture dish with the diameter of 9cm, and respectively inoculating pathogenic fungus cyan 9A-2-6 (5 mm) with the diameter at the opposite sideFusarium tricinctum) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And 9A-4-13 (Fusarium acuminatum) The treatment was repeated 3 times for each pathogenic fungus, and the control was made of the treatment with only the pathogenic fungus. Culturing in 28 deg.C biochemical incubator for 7 days, observing whether there is bacteria inhibition zone between strain separated from fermented grains of highland barley Chinese liquor and pathogenic fungi, measuring colony diameter, and calculating bacteria inhibition rate, wherein the calculation formula is as follows:
inhibition (%) = [ (control colony diameter-treated colony diameter)/control colony diameter ] × 100
The results are shown in table 2, fig. 2 and fig. 3.
TABLE 2 Activity of Siamese Bacillus JZ1-4-10 against Fusarium
Figure 780941DEST_PATH_IMAGE002
Note: the values in the table are mean ± sd, and different lower case letters represent significant differences at P <0.05 levels after the same column of data.
In order to determine the stability of the active strains, the stability evaluation is carried out on the strains with the bacteriostatic activity obtained by primary screening, and the method is the same as the above method. Respectively with the Siamese bacillus of this application and fusarium confrontation cultivation 7d and 14d after, measure antibacterial bandwidth width, antibacterial bandwidth width reduces rate (VF) when calculating 14d, compares Siamese bacillus to various fusarium's suppression stability.
The stability evaluation criteria were: "- -": VF is more than or equal to 70 percent and is unstable; "-": VF more than or equal to 50 percent and less than 70 percent, is relatively unstable; "+": VF being more than or equal to 20 percent and less than 50 percent is more stable; "++": VF is less than 20 percent and is stable. The calculation formula is as follows:
VF (%) = [ (7d antibacterial bandwidth value-14 d antibacterial bandwidth value)/7 d antibacterial bandwidth value ] × 100
The results are shown in Table 3:
TABLE 3 stability of Siamese Bacillus JZ1-4-10 against pathogenic bacteria
Figure DEST_PATH_IMAGE003
As can be seen from Table 3, the Siamese bacillus JZ1-4-10 strain of the application has stable bacteriostatic action on fusarium solani 9A-2-6, 9A-5-7 and 9A-5-10, and the stability is '+'; has stable bacteriostatic action on the blue 9A-4-13, and the stability is plus.
Determination of prevention and treatment effect of Siamese bacillus JZ1-4-10 on potato dry rot
Firstly inoculating activated Siamese bacillus JZ1-4-10 in ATCC213 modified liquid culture medium, and inoculating at 37 ℃ for 200 r min-1Shake-culturing in shake flask cabinet for 2d, and collecting fermentation liquid. The concentration of JZ1-4-10 strain fermentation broth was adjusted to 1.0X 10 using a hemocytometer8 cfu·mL-1(ii) a Inoculating 4 kinds of Fusarium causing potato dry rot in PDA liquid culture medium, respectively, and culturing at 28 deg.C and 200 r min-1Shake-culturing in shake flask cabinet for 2d, and collecting fermentation liquid. The spore concentration of 4 kinds of fusarium fermentation liquor is respectively adjusted to 1.0 multiplied by 10 by a blood counting chamber7each.mL-1.2 orifices 3mm deep were then punched in the sterilized potatoes with a 5mm diameter punch. Each orifice is firstly connected with 40 mu L fusarium fermentation liquor, and then is connected with 40 mu L SiameseThe bacillus fermentation broth was used as the experimental group. Wrapping the inoculated potatoes with a sealing film, putting the wrapped potatoes into a self-sealing bag, and culturing for 14d in a constant-temperature incubator at the temperature of 28 ℃ and the relative humidity of about 75%. Treating fusarium inoculation fermentation liquid and equivalent sterile water as positive control; the treatment with the same amount of sterile water and the Siamese bacillus fermentation liquor is used as a negative control, and the treatment with only the same amount of sterile water is used as a blank control. One potato was inoculated per treatment and repeated 3 times.
After the potato tubers are cultured for 14 days, the fruit failure rate, the lesion depth and the inhibition rate of the potato tubers treated by the fermentation liquor and the extract solution are respectively measured, and the inhibition rate calculation formula is as follows.
Inhibition (%) = LP-LA/LP × 100%.
Wherein LP is the potato tuber quality of the blank control group-the potato tuber quality of the positive control group, and LA is the potato tuber quality of the negative control group-the potato tuber quality of the experimental group. The results are shown in Table 4 below,
TABLE 4 inhibitory Effect of Siamese Bacillus JZ1-4-10 on Fusarium
Figure 760399DEST_PATH_IMAGE004
As can be seen from the table, the inhibitory activities of the JZ1-4-10 fermentation broth on 4 kinds of fusarium are respectively cyan 9A-2-6, cyan 9A-5-7, cyan 9A-4-13 and cyan 9A-5-10 from high to low. Wherein the rate of bad fruits of the JZ1-4-10 fermentation liquor on fusarium wilt blue 9A-2-6 is 0.78%, the depth of disease spots is 0.69 cm, and the inhibition rate reaches 75.33%; the rate of bad fruits for the green 9A-5-7 is 0.35 percent, the depth of the disease spot is 0.89 cm, and the inhibition rate reaches 66.15 percent; the rate of bad fruits for the green 9A-4-13 is 0.62%, the depth of the disease spot is 0.93 cm, and the inhibition rate is 60.85%; the rate of bad fruits for green 9A-5-10 is 0.49%, the depth of lesion spots is 1.25 cm, and the inhibition rate reaches 43.26%.
Example 3
Preparation of Siamese bacillus JZ1-4-10 fermentation liquor and extract thereof:
inoculating Siamese bacillus JZ1-4-10 strain into an ATCC213 modified liquid culture medium with the bottling volume of 400mL, and placing at 37 ℃ for 180r min-1Shaking culture in constant temperature shaking flask cabinetAnd 5-7 d, harvesting the fermentation liquor, and then filtering the fermentation liquor under reduced pressure by using a Buchner funnel to remove thalli to obtain a thallus-removed fermentation liquor for later use.
Placing 600mL of the thallus-removed fermentation liquor into a 3000mL separating funnel, sequentially extracting the fermentation liquor by using chloroform, ethyl acetate and n-butanol which are equal in volume, and carrying out rotary evaporation on the extract to obtain an extract; the extract was prepared with sterile water to mass concentrations of 1.00, 5.00, 10.00 and 20.00 mg/mL, respectively-1The extract solution of (2) is filtered by a 0.22 μm microporous membrane for later use.
The same method as that for the fermentation broth in example 2 was used to verify the inhibitory effect of the fermentation broth extract on the pathogens of potato dry rot, and the results are shown in table 5.
TABLE 5 inhibitory Effect of Siamese Bacillus JZ1-4-10 extract on pathogenic bacteria of dried rot disease of potato
Figure DEST_PATH_IMAGE005
As can be seen from Table 5, the n-butanol extract of the fermentation broth of JZ1-4-10 strain had the highest inhibitory activity against 4 Fusarium species causing potato dry rot, the ethyl acetate extract had the second highest activity, and the chloroform extract had the lowest activity. Wherein the content of n-butanol extract is 10 mg/mL-1The inhibition rate of the fusarium wilt enzyme 9A-2-6 is up to 70.25% under the test concentration; at 20 mg. mL-1The inhibition rates of the fusarium graminearum 9A-2-6 and the fusarium graminearum 9A-5-7 are respectively as high as 78.00% and 72.5% under the test concentration; the inhibition rate of the fusarium wilt enzyme 9A-4-13 is 58.00%. The ethyl acetate extract was 10 mg/mL-1The inhibition rate of the test solution on the pathogenic bacteria cyan 9A-2-6 is 56.00 percent; at 20 mg. mL-1The inhibition rates of the test solution on pathogenic bacteria cyan 9A-2-6 and cyan 9A-4-13 are respectively up to 77.34% and 66.50%.
10 mg·mL-1And 20 mg. mL-1The ethyl acetate extract and the n-butanol extract under all the tested concentrations have stronger inhibition effects on the pathogenic bacteria blue 9A-2-6, and the inhibition rate ranges from 56.00% to 78.00%. Only 20 mg. mL-1The n-butanol extract has strong inhibition effect on pathogenic bacteria blue 9A-5-7The preparation rate is as high as 72.50%. Only 20 mg. mL-1The ethyl acetate and n-butanol extracts have strong inhibition effect on pathogenic bacteria cyan 9A-4-13, and the inhibition rate reaches 66.50% and 58.00%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Sequence listing
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<120> Siamese bacillus, microbial inoculum, extract and application thereof
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ggttgtttga accgcatggt tcagacataa aaggtggctt cggctaccac ttacagatgg 180
acccgcggcg cattagctag ttggtgaggt aacggctcac caaggcgacg atgcgtagcc 240
gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg 300
cagcagtagg gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga 360
tgaaggtttt cggatcgtaa agctctgttg ttagggaaga acaagtgccg ttcaaatagg 420
gcggcacctt gacggtacct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 480
taatacgtag gtggcaagcg ttgtccggaa ttattgggcg taaagggctc gcaggcggtt 540
tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactggggaa 600
cttgagtgca gaagaggaga gtggaattcc acgtgtagcg gtgaaatgcg tagagatgtg 660
gaggaacacc agtggcgaag gcgactctct ggtctgtaac tgacgctgag gagcgaaagc 720
gtggggagcg aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctaag 780
tgttaggggg tttccgcccc ttagtgctgc agctaacgca ttaagcactc cgcctgggga 840
gtacggtcgc aagactgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 900
tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tctgacaatc 960
ctagagatag gacgtcccct tcgggggcag agtgacaggt ggtgcatggt tgtcgtcagc 1020
tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc 1080
agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg 1140
acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggacagaaca 1200
aagggcagcg aaaccgcgag gttaagccaa tcccacaaat ctgttctcag ttcggatcgc 1260
agtctgcaac tcgactgcgt gaagctggaa tcgctagtaa tcgcggatca gcatgccgcg 1320
gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1380
cgaagtcggt gaggtaacct ttatggagcc agcc 1414

Claims (9)

1. The Siamese bacillus is characterized in that the Siamese bacillus JZ1-4-10 is preserved in Guangdong province microorganism strain preservation center in 2020, 10 and 15 days, and the preservation number is as follows: GDMCC No: 61236.
2. a microbial inoculant comprising bacillus siamensis JZ1-4-10 of claim 1.
3. A siamese bacillus fermentation liquor extract, characterized in that the extract is obtained by culturing the siamese bacillus of claim 1 to obtain a fermentation liquor, and then extracting the fermentation liquor with an organic solvent.
4. The Siamese Bacillus fermentation broth extract according to claim 3, wherein the organic solvent is one of chloroform, ethyl acetate and n-butanol.
5. The Siamese Bacillus fermentation broth extract according to claim 3, wherein the culture medium used for culturing the Siamese Bacillus is ATCC213 modified liquid culture medium.
6. Use of the bacillus siamensis JZ1-4-10 of claim 1, the microbial inoculum of claim 2 or the extract of claim 3 for inhibiting fusarium.
7. The use of claim 6, wherein said Fusarium is cyan 9A-2-6 (C.)Fusarium solani) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) Or 9A-4-13 (Fusarium acuminatum）。
8. Use of the bacillus siamensis JZ1-4-10 of claim 1, the microbial inoculum of claim 2 or the extract of claim 3 in preventing and controlling the growth of the green blue 9A-2-6 (6)Fusarium solani) 9A-5-7 (Fusarium equiseti) 9A-5-10 (Fusarium incarnatum) And/or 9A-4-13 (Fusarium acuminatum) The application in the dry rot caused by the disease.
9. Use according to claim 8, wherein the dry rot is potato dry rot.
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CN113201478A (en) * 2021-05-27 2021-08-03 广西民族大学 Siamese bacillus Gxun-36 secreting iron carrier and application thereof
CN114891670A (en) * 2022-04-28 2022-08-12 岭南师范学院 Bacillus siamensis ZJh with broad-spectrum antibacterial activity and application thereof

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CN111690578A (en) * 2020-07-31 2020-09-22 山东佐田氏生物科技有限公司 Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof
CN111849844A (en) * 2020-09-02 2020-10-30 青海省农林科学院 Bacillus licheniformis A10201 and application thereof
CN111876344A (en) * 2020-06-29 2020-11-03 广西大学 24-membered ring macrolide high-yield strain and application thereof

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CN111876344A (en) * 2020-06-29 2020-11-03 广西大学 24-membered ring macrolide high-yield strain and application thereof
CN111690578A (en) * 2020-07-31 2020-09-22 山东佐田氏生物科技有限公司 Salt and alkali resistant Siamese bacillus and production method and application of viable bacteria preparation thereof
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CN113201478A (en) * 2021-05-27 2021-08-03 广西民族大学 Siamese bacillus Gxun-36 secreting iron carrier and application thereof
CN113201478B (en) * 2021-05-27 2022-07-29 广西民族大学 Siamese bacillus Gxun-36 secreting iron carrier and application thereof
CN114891670A (en) * 2022-04-28 2022-08-12 岭南师范学院 Bacillus siamensis ZJh with broad-spectrum antibacterial activity and application thereof
CN114891670B (en) * 2022-04-28 2023-09-19 岭南师范学院 Siamese bacillus ZJh with broad-spectrum antibacterial property and application thereof

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