CN113151079A - Paenibacillus polymyxa KDB and application thereof - Google Patents
Paenibacillus polymyxa KDB and application thereof Download PDFInfo
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a Paenibacillus polymyxa KDB and application thereof, belonging to the technical field of biology. The invention separates an endophytic bacterium from a millet variety purple stalk white, the endophytic bacterium is identified as paenibacillus polymyxa (Paenibacillus polymyxa) through molecules, the strain is named KDB, the strain is preserved in the common microorganism center of China Committee for culture Collection of microorganisms in 25 months in 2021, the preservation address is No.3 of Western Lu No.1 of the Korean district in Beijing, the taxonomic name is paenibacillus polymyxa (Paenibacillus polymyxa), and the preservation number is CGMCC No. 22073. The strain is found to have an inhibition rate of 87.82% on the blast millet bacteria (Pyricularia oryzae) in the culture of the plate confronting each other, and has good inhibition effects on various fungi such as the fusarium graminearum and the fusarium oxysporum.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a Paenibacillus polymyxa KDB and application thereof.
Background
Millet is a coarse cereal crop with Chinese characteristics, has a long history, is rich in nutrition, is drought-resistant and barren-resistant, and has a very good development prospect. The blast disease is a common and serious-harm epidemic disease in a millet planting area, the pathogenic bacteria of the blast disease is Pyricularia oryzae (Pyricularia oryzae), the blast disease can be caused in the whole growth period, particularly, the spike blast in the later period has great influence on the yield, and the serious occurrence can cause the failure of production. In recent years, because of the lack of disease-resistant varieties in production and the large-area planting of single varieties, blast diseases of millet have become one of important diseases affecting the production of millet in China. The most economical and effective method for preventing and treating the blast disease is to use disease-resistant varieties, but physiological races of the blast disease bacteria are frequently changed, so that the disease resistance of hosts is lost. The pesticide for preventing and treating the paddy rice blast is easy to generate pesticide resistance, pollutes the environment, increases the production cost of farmers, and is not beneficial to the production of green millet, so other methods are required to be found for preventing and treating the paddy rice blast. Biological control is a very promising development direction because it does not pollute the environment. Various biocontrol strains have been discovered and relevant application studies have been conducted on individual crops.
Endophytes are a class of microorganisms that grow in plants without causing any symptoms. In the long-term evolution process, the two are adapted and interacted with each other, and play an important role in preventing and controlling plant diseases. For example, Zabihulla separates a strain of endophytic Bacillus amyloliquefaciens 489-2-2 from cotton, the strain can inhibit the growth of verticillium dahliae filaments of cotton, and the disease control effect is better through seed soaking and root irrigation. The endophytic bacillus amyloliquefaciens RJ-4 strain is separated from sarcandra glabra and the like, has good prevention and control effects on sarcandra glabra anthracnose, can secrete protease, chitinase and the like, and the fermentation liquor can treat anthracnose hyphae to enable the hyphae to be twisted, broken and inhibited from growing.
Because of the easy reproduction of bacillus, strong stress resistance, wide bacteriostasis spectrum, long shelf life and other characteristics, a plurality of products are used for preventing and treating plant diseases. The paenibacillus polymyxa secretes a natural antibacterial substance, so that the paenibacillus polymyxa has a good effect in preventing and treating plant diseases. If the paenibacillus polymyxa KM2501-1 strain is obtained by once separation, the strain fermentation liquor is used for preventing and treating tomato root-knot nematode, and the prevention effect is equivalent to the effect of abamectin. A biocontrol bacterium HK18-8 is separated from the rhizosphere soil of the champignon in a peaceful manner and is identified as paenibacillus polymyxa, the strain can inhibit the growth of hyphae and the spore germination of the colletotrichum capsici, and the control effect of an in vitro inoculation experiment can reach 88.58%. Theobroma cacao black clamp rot is an important disease of Theobroma cacao in Mexico, is caused by Phytophthora tropicalis (Phytophthora tropicalis), and causes great harm to local cocoa planting. Ch & vezRami i rez utilizes the paenibacillus polymyxa NMA1017 strain to prevent and treat the disease, the experimental result shows that after the biocontrol bacteria is sprayed in the field, the morbidity is reduced to 6% from 68%, and the indoor antagonism experiment shows that the biocontrol bacteria inhibition rate is 85.9%, and the biocontrol bacteria cell wall is damaged. At present, people often select pesticides to control millet diseases, and biological control reports are few, the invention separates a bacillus polymyxa KDB from healthy leaves of a millet blast resistant variety purple stalk white, and the bacillus polymyxa KDB is cultured by culture dishes in opposition to each other, so that the bacillus polymyxa KDB can inhibit the growth of millet blast bacteria hyphae, has a wide antibacterial spectrum, and has strong inhibition on curvularia vularia lunata, sclerotinia sclerotiorum, botrytis cinerea, rhizoctonia cerealis, sesamum frutescens, pestalotiopsis cerealis and fusarium graminearum. The fermentation liquor is used for treating the blast disease bacterium conidia for moisturizing culture, and the fermentation liquor can well inhibit the germination of the blast disease bacterium conidia. The control effects of the fermentation liquor of the paenibacillus polymyxa KDB on the leaf blast and the panicle blast respectively reach 68.16 percent and 66.78 percent in pot experiments, the fermentation liquor has the potential of further developing biocontrol products, and can be used for preventing and treating the panicle blast in the future.
Disclosure of Invention
One of the purposes of the invention is to provide paenibacillus polymyxa which is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.22073 and the preservation address of No.3 Hospital No.1 of Beijing Korean district, Beichen Xilu.
The second purpose of the invention is to provide an engineering bacterium obtained by genetic improvement of paenibacillus polymyxa with the preservation number of CGMCC No. 22073.
Wherein, the engineering bacteria are targeted at the bacillus of the invention, and the adopted means is generally to transfer and/or knock out specific genes and/or sequences and the like into the bacillus, so the engineering bacteria are still the bacillus or the paenibacillus polymyxa. In addition, the engineering bacteria can be engineering bacteria with improved antibacterial activity on fusarium graminearum, rhizoctonia solani, pestalotiopsis microsporus, curvularia vularia lunata, botrytis cinerea, rhizoctonia solani, rhizoctonia cerealis, sclerotinia sclerotiorum, pyricularia graminis and sesamonia graminis, and can also be engineering strains with other pest and disease activities.
The invention also aims to provide a composition which comprises the paenibacillus polymyxa and/or the engineering bacteria.
The fourth object of the present invention is to provide a pesticidal preparation comprising the above-mentioned Paenibacillus polymyxa and/or the above-mentioned engineering bacterium.
The fifth purpose of the invention is to provide the application of the paenibacillus polymyxa, the engineering bacteria or the composition in preparing pesticide preparations.
Preferably, the application is that the paenibacillus polymyxa, the engineering bacteria or the composition is used for preventing and treating at least one of fusarium graminearum, rhizoctonia solani, pestalotiopsis microsporus, curvularia lunata, botrytis cinerea, rhizoctonia zeae, rhizoctonia cerealis, sclerotinia sclerotiorum, pyricularia gramineara and fusarium oxysporum.
In the present invention, the term "protection" means protection or mitigation of a target organism from a pest or the like by preventing and/or treating the pest or the like produced by the target organism.
The sixth purpose of the present invention is to provide a preparation method of the paenibacillus polymyxa fermentation liquid, which comprises the following steps: shaking in liquid fermentation medium at 25-30 deg.C for 45-50 hr.
Preferably, the shaking table is oscillated at a speed of 180 rpm/min.
More optionally, the liquid fermentation medium comprises the following components: 200 g of potato, 20 g of glucose and 1000mL of distilled water.
Compared with the prior art, the invention has the following beneficial effects:
the paenibacillus polymyxa KDB separated from healthy anti-sitz variety purple stalk white leaves has the bacteriostasis rate of 87.82% to the Pyricularia oryzae, the prevention and treatment effect of 68.16% to the phyllosphere and the prevention and treatment effect of 66.78% to the sitobis, and simultaneously has the growth inhibition effect on nine germs, namely, the curvularia lunata, the sclerotinia sclerotiorum, the rhizoctonia cerealis, the sesamum fruticosa, the pestalotia cerealis, the pestalotia microcarpa, the rhizoctonia solani, the rhizoctonia cerealis and the fusarium graminearum, and the inhibition rate is more than 60%, so that the broad spectrum is better, and the invention has good application prospect.
Biological deposit description of paenibacillus polymyxa KDB:
the preservation organization: china general microbiological culture Collection center;
the preservation date is as follows: 03 month 25, 2021;
the preservation number is: CGMCC No. 22073;
and (4) storage address: xilu No.1 Hospital No.3, Beijing, Chaoyang, North;
taxonomic nomenclature: paenibacillus polymyxa (Paenibacillus polymyxa).
Drawings
FIG. 1 is a phylogenetic tree of the 16S rDNA sequence of the strain KDB of example 2.
FIG. 2 is a graph showing the bacteriostatic results of the strain KDB in example 3 against Valeriana fauriei Keng.
FIG. 3 is a graph showing the bacteriostatic results of the strain KDB in example 3 on 10 different pathogenic bacteria.
FIG. 4 is a graph showing the inhibitory effect of fermentation broth of the strain KDB in example 4 on spore germination and appressorium formation of pestivirus cerealis.
Detailed Description
EXAMPLE 1 isolation and cultivation of the Strain KDB
0.5g of sample of anti-Valley blast variety purple stalk white healthy leaves collected from a Shijiazhuangyu xi horse experiment station is disinfected by 75% alcohol for 30s, washed by sterilized water for 3 times, cut into pieces by using disinfected scissors and then put into a mortar for grinding, a proper amount of water and carborundum are added into the mortar for grinding, and the grinding fluid is centrifuged to obtain supernatant, namely the bacterial suspension. Sequentially diluting the obtained bacterial suspension to 10-1、10-2、10-3、10-4、10-5Then 100ul of the strain is respectively smeared on an LB medium plate, the strain is cultured for 2d at 28 ℃ in an incubator (the preparation method of the LB medium comprises the steps of 10g of tryptone, 5g of yeast extract, 17g of agar powder, 10g of NaCl, 5mol/LNaOH, pH adjustment to 7.0, adding 1000mL of distilled water, and high-pressure steam sterilization at 121 ℃ for 20min), then a single colony is selected, the strain is purified by adopting a line drawing method, the strain is cultured in an opposite way with the Valsa, and antagonistic strains are screened from the strain. Screening shows that one strain has strong inhibition on the blast furnace blight, is named as KDB, and is subjected to strain identification.
Example 2 identification of the Strain KDB molecule
Performing molecular detection on the strain: carrying out amplification on KDB strain in LB liquid culture medium, carrying out PCR amplification by using 2 mul KDB bacterial liquid as template, wherein the reaction system is 25 mul, the primers are respectively 0.5 mul and Mix is 12.5 mul, and sterilizing ddH2O9.5. mu.l, and 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ IN NO. 1); 1495R: 5'-CTACGGCTACCTTGTTACGA-3' (SEQ IN NO.2) for amplifying the 16S rRNA gene of bacteria. And (3) amplification procedure: pre-denaturation at 94 deg.C for 10min, denaturation at 94 deg.C for 30s, annealing at 55 deg.C for 30s, extension at 72 deg.C for 2min, 30 cycles, extension at 72 deg.C for 10min, and storage at 4 deg.C. Detection of amplified nodules by 1% agarose gel electrophoresisAnd (3) recovering the target strip by using glue, connecting the target strip to a T vector, transforming the T vector to escherichia coli DH5a by using a heat shock method, coating the obtained product on an LB (lysogeny broth) plate containing ampicillin for overnight culture, selecting a single colony for colony PCR (polymerase chain reaction) detection, and sending the positively detected clone to Beijing Zhongkocillin company for sequencing. The results of the alignment analysis of the 16S rRNA gene (the sequence is shown in SEQ ID NO. 3) of the KDB strain and other Paenibacillus species by MEGA6.0 software are shown in FIG. 1, and the KDB strain and the Paenibacillus polymyxa (Paenibacillus polymyxa) can be clearly seen from the phylogenetic tree of FIG. 1, and the KDB strain is identified as the Paenibacillus polymyxa (Paenibacillus polymyxa).
Example 3 detection of bacteriostatic action by plate confrontation method
Inoculating the stored sitophil blight mycelia on a PDA (personal digital assistant) plate for activation, standing and culturing at the constant temperature of 28 ℃ for 9 days, growing the mycelia on a culture dish, punching holes on the edges of bacterial colonies by using a sterilized puncher, picking out bacterial cakes and placing the bacterial cakes in the center of a new Plate (PDA), respectively streaking and inoculating a paenibacillus polymyxa strain KDB at two positions which are about 3cm away from the bacterial cakes, taking a plate which is only inoculated with sitophil blight bacteria and is not inoculated with the strain KDB as a control, placing the inoculated plate in the same incubator for dark culture at the temperature of 28 ℃ for 7 days, observing the growth condition of the sitophil in the plate, measuring the diameter of the bacterial colonies, and seeing that the paenibacillus polymyxa strain KDB obviously inhibits the growth of the sitophil blight bacteria, wherein the result is shown in figure 2.
The antagonism of the bacterial strain KDB to the nine germs of the curvularia lunata, sclerotinia sclerotiorum, botrytis cinerea, rhizoctonia cerealis, fusarium graminearum, paecilomyces microsporum, rhizoctonia zeae, rhizoctonia cerealis and fusarium graminearum is detected according to the method, the results of calculation of the bacteriostatic ratio are shown in Table 1, and the bacteriostatic effects are shown in FIG. 3 (A, B, C, D, E, F, G, H, I, J in FIG. 3 represents the inhibitory effect maps of the strain KDB against Fusarium graminearum, Rhizoctonia solani, Pestalotiopsis microplasma, Curvularia lunata, Botrytis cinerea, Rhizoctonia zeae, Rhizoctonia cerealis, Sclerotinia sclertiorum, Pyricularia graminis, Pyricularia oryzae, and Bipolaris setaria), respectively, and it can be seen from FIG. 3 that the strain KDB significantly inhibits the growth of these bacteria. As can be seen from Table 1, the strain has the best inhibition effect on the fusarium graminearum which is 87.82%, and the secondary bacteria are the fusarium oxysporum, sclerotinia sclerotiorum, botrytis cinerea, rhizoctonia cerealis, fusarium oxysporum, pestalotiopsis microsporus, rhizoctonia zeae, rhizoctonia cerealis and fusarium graminearum.
Wherein the relative inhibition ratio is (control colony diameter-test strain treated colony diameter)/control colony diameter
TABLE 1 bacteriostatic Activity of Strain KDB
Example 4 Effect of the Strain KDB on spores of Gluconobacter
Inoculating KDB strain into liquid PDB culture medium (PDB culture medium preparation method: potato 200 g, glucose 20 g, distilled water 1000mL, 121 deg.C high pressure steam sterilization for 20min), shaking in shaking table at 28 deg.C of 180rpm for 48 hr, collecting fermentation broth, centrifuging to remove precipitate, filtering with filter paper, sterilizing the filtrate with 0.22 μm bacterial filter, collecting the same amount of fermentation broth and 1 × 106Mixing the individual/ml of the blasticidium graminearum suspension, simultaneously mixing the equal volume of the PDB culture medium with the spore suspension as a control, sucking 10 mu l of the mixed solution after mixing, dripping the mixed solution on hydrophobic glass paper, putting the hydrophobic glass paper in a culture dish for wet culture, and observing the germination condition of spores after 24 hours of culture.
Through microscopic observation, the blast fever conidia of the control spore suspension germinate and form black attached cells, while the blast fever conidia treated by the fermentation broth only have about 20 percent of spores capable of germinating, and the germ tubes formed by the germinated blast fever spores are short and do not form the attached cells, so that the blast fever conidia treated by the fermentation broth can obviously reduce the germination and the formation of the attached cells, and the result is shown in fig. 4, wherein A is a control group, and a PDB liquid medium treats the blast fever germs; and B is a treatment group, and the bacterial strain KDB fermentation liquor is used for treating the germs of the paddy rice blast.
Example 5 controlling Effect of the Strain KDB on Valley blast
(1) Preparation of blast disease bacterial spore suspension: activating the blast bacteria, transferring to a sorghum grain culture medium, culturing for about 10 days, washing the sorghum grains with hyphae with tap water, drying, placing on 2 layers of wet filter paper paved in an enamel disc, covering with 2 layers of gauze, and culturing at 25 ℃ for 5 days to promote spore production. Eluting conidia with distilled water after spore production to obtain a concentration of 1 × 105Tween 20 was added to a final concentration of 0.05% to the spore suspension.
(2) The test on the prevention and treatment effect of the strain KDB on the leaf blast comprises the following steps: sterilizing the flower pot and nutrient soil, planting susceptible variety Jigu 19, retaining 3-4 seedlings in each pot, and culturing in light incubator. After 30 days, when the seedlings grow to 6-7 leaf stage, the test groups use the fermentation liquor of the paenibacillus polymyxa strain KDB for spray inoculation (the preparation of the fermentation liquor of KDB is the same as that in example 4), and 3 times of repeated inoculation are set; the control group was sprayed with sterile water, taking care to preserve moisture. And after inoculating for 1d, carrying out blast disease bacterial spore suspension spray inoculation, keeping moisture in the dark for 24 hours, then transferring to an illumination incubator for culture, spraying water for 3 times every day to keep moisture, observing the disease condition of the leaves after 15d, and calculating the disease index and the prevention and treatment effect of the leaves.
(3) The test on the prevention and treatment effect of the strain KDB on the panicle blast comprises the following steps: when the millet enters the heading stage, spraying the millet ears by using a paenibacillus polymyxa strain KDB fermentation broth (the preparation of the KDB fermentation broth is the same as that in example 4) by a test group, and setting 3 times of repetition; the control group was sprayed with sterile water, taking care to preserve moisture. And after 1d, uniformly spraying a blast disease spore suspension on all the treatments, putting all the treatments in the same incubator for dark moisturizing, moving the incubators to an illumination incubator for culturing after 24h, observing the disease condition of the ears at the later stage of grouting, and calculating the disease index and the prevention and treatment effect. The results are shown in Table 2.
Disease index ═ Σ (number of diseased plants at each stage × disease value)/(total number of investigated plants × highest value) × 100
Control effect (%) (1- (disease index in treatment area/disease index in control area)) × 100
TABLE 2 prevention and treatment effects of KDB strain on leaf blast and ear blast
Therefore, the control effect of the biocontrol strain on leaf blast and panicle blast reaches 68.16% and 66.78% respectively.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Sequence listing
<110> millet institute of academy of agriculture, forestry and science of Hebei province
<120> Paenibacillus polymyxa KDB and application thereof
<130> 2021.04.14
<141> 2021-04-19
<160> 3
<170> SIPOSequenceListing 1.0
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<213> Artificial sequence ()
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agagtttgat cctggctcag 20
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<213> Artificial sequence ()
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<211> 1501
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<213> Paenibacillus polymyxa (Paenibacillus polymyxa KDB 16SrDNA sequence)
<400> 3
agagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgagc 60
gaggttgttt agaagcttgc ttctaaacaa cctagcggcg gacgggtgag taacacgtag 120
gcaacctgcc cacaagacag ggataactac cggaaacggt agctaatacc cgatacatcc 180
ttttcctgca tgggagaggg aggaaagacg gagcaatctg tcacttgtgg atgggcctgc 240
ggcgcattag ctagttggtg gggtaaaggc ctaccaaggc gacgatgcgt agccgacctg 300
agagggtgat cggccacact gggactgaga cacggcccag actcctacgg gaggcagcag 360
tagggaatct tccgcaatgg gcgaaagcct gacggagcaa cgccgcgtga gtgatgaagg 420
ttttcggatc gtaaagctct gttgccaggg aagaacgtct tgtagagtaa ctgctacaag 480
agtgacggta cctgagaaga aagccccggc taactacgtg ccagcagccg cggtaatacg 540
tagggggcaa gcgttgtccg gaattattgg gcgtaaagcg cgcgcaggcg gctctttaag 600
tctggtgttt aatcccgagg ctcaacttcg ggtcgcactg gaaactgggg agcttgagtg 660
cagaagagga gagtggaatt ccacgtgtag cggtgaaatg cgtagagatg tggaggaaca 720
ccagtggcga aggcgactct ctgggctgta actgacgctg aggcgcgaaa gcgtggggag 780
caaacaggat tagataccct ggtagtccac gccgtaaacg atgaatgcta ggtgttaggg 840
gtttcgatac ccttggtgcc gaagttaaca cattaagcat tccgcctggg gagtacggtc 900
gcaagactga aactcaaagg aattgacggg gacccgcaca agcagtggag tatgtggttt 960
aattcgaagc aacgcgaaga accttaccag gtcttgacat ccctctgacc gctgtagaga 1020
tatggctttc cttcgggaca gaggagacag gtggtgcatg gttgtcgtca gctcgtgtcg 1080
tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta tgcttagttg ccagcaggtc 1140
aagctgggca ctctaagcag actgccggtg acaaaccgga ggaaggtggg gatgacgtca 1200
aatcatcatg ccccttatga cctgggctac acacgtacta caatggccgg tacaacggga 1260
agcgaaatcg cgaggtggag ccaatcctag aaaagccggt ctcagttcgg attgtaggct 1320
gcaactcgcc tacatgaagt cggaattgct agtaatcgcg gatcagcatg ccgcggtgaa 1380
tacgttcccg ggtcttgtac acaccgcccg tcacaccacg agagtttaca acacccgaag 1440
tcggtggggt aacccgcaag ggagccagcc gccgaaggtg gggtagatga ttggggtgaa 1500
g 1501
Claims (9)
1. The paenibacillus polymyxa is characterized in that the paenibacillus polymyxa is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.22073 and the preservation address of No.3 Siro No.1 of Beijing republic of the rising district.
2. An engineered bacterium obtained by genetically modifying the Paenibacillus polymyxa of claim 1.
3. A composition comprising paenibacillus polymyxa according to claim 1 and/or an engineered bacterium according to claim 2.
4. A pesticide formulation comprising paenibacillus polymyxa according to claim 1 and/or an engineered bacterium according to claim 2.
5. Use of the paenibacillus polymyxa according to claim 1, the engineered bacterium according to claim 2 or the composition according to claim 3 for the preparation of a pesticide formulation.
6. The use of claim 5, wherein the use is of the Paenibacillus polymyxa, the engineering bacteria or the composition for controlling at least one of Fusarium graminearum, Rhizoctonia cerealis, Pestalotiopsis microphylla, Curvularia pombe, Botrytis cinerea, Rhizoctonia zeae, Rhizoctonia cerealis, Sclerotinia sclerotiorum, Sclerotinia graminis, Petasium graminearum, and Petasium vulgare.
7. A method of preparing a fermentation broth of Paenibacillus polymyxa according to claim 1, comprising the steps of: shaking in liquid fermentation medium at 25-30 deg.C for 45-50 hr.
8. The method of claim 7, wherein the shaking table is oscillated at a speed of 180 rpm/min.
9. The method of preparing a paenibacillus polymyxa fermentation broth of claim 8, wherein the liquid fermentation medium comprises the following components: 200 g of potato, 20 g of glucose and 1000mL of distilled water.
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| CN102250815A (en) * | 2011-07-21 | 2011-11-23 | 中国热带农业科学院香料饮料研究所 | Endogenous paenibacillus polymyxa |
| CN105018388A (en) * | 2015-07-31 | 2015-11-04 | 长江大学 | Paenibacillus polymyxa and application thereof |
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| CN109762777A (en) * | 2019-04-10 | 2019-05-17 | 鲁东大学 | One plant of Paenibacillus polymyxa bacterial strain and its application |
| CN110628686A (en) * | 2019-10-28 | 2019-12-31 | 云南省农业科学院生物技术与种质资源研究所 | Bacillus polymyxa strain and application thereof |
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| CN102250815A (en) * | 2011-07-21 | 2011-11-23 | 中国热带农业科学院香料饮料研究所 | Endogenous paenibacillus polymyxa |
| US20170303544A1 (en) * | 2014-08-08 | 2017-10-26 | Universidad De Santiago De Chile | Paenibacillus polymyxa schc 33 bacterial strain, and use thereof to combat phytopathogenic fungi in fruits, vegetables or plants |
| CN105018388A (en) * | 2015-07-31 | 2015-11-04 | 长江大学 | Paenibacillus polymyxa and application thereof |
| CN109762777A (en) * | 2019-04-10 | 2019-05-17 | 鲁东大学 | One plant of Paenibacillus polymyxa bacterial strain and its application |
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| CN114736825B (en) * | 2022-04-12 | 2023-12-19 | 慕恩（广州）生物科技有限公司 | Paenibacillus polymyxa, biochemical preparation and application thereof |
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