CN113145314A - Electrophoresis screening device of charged polydisperse polymer microspheres - Google Patents
Electrophoresis screening device of charged polydisperse polymer microspheres Download PDFInfo
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- CN113145314A CN113145314A CN202110338198.4A CN202110338198A CN113145314A CN 113145314 A CN113145314 A CN 113145314A CN 202110338198 A CN202110338198 A CN 202110338198A CN 113145314 A CN113145314 A CN 113145314A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C7/00—Separating solids from solids by electrostatic effect
- B03C7/02—Separators
Abstract
The invention relates to the technical field of microsphere processing, and discloses an electrophoresis screening device for charged polydisperse polymer microspheres, which is characterized in that: the device comprises an emulsion pool with a vibration device and at least one group of conveying and screening units which are communicated through a microsphere conveying pipeline; each group of conveying and screening units comprises a conveying pump and an electric field pool which are sequentially arranged along the flowing direction of the charged polydisperse microspheres to be screened; on the microsphere conveying pipeline, the emulsion pool and each group of conveying and screening units are sequentially arranged along the flowing direction of charged polydisperse microspheres to be screened; and the emulsion pool is communicated with the electric field pools in the last group of conveying and screening units through the microsphere conveying pipeline. Compared with the prior art, the invention has the advantages of low price, simple and convenient operation method, environmental protection, high efficiency and environmental protection, and can obtain the monodisperse microspheres with different particle sizes by screening from the monodisperse microspheres more simply and conveniently.
Description
Technical Field
The invention relates to the technical field of microsphere processing, in particular to an electrophoresis screening device for charged polydisperse polymer microspheres.
Background
The polymer microspheres refer to polymer particles with diameters ranging from tens of nanometers to hundreds of micrometers. The small particle size and volume allows the entire particle to have a fast response rate to external stimuli when used as a microreactor. The high-quality monodisperse polymer microsphere has the characteristics of large specific surface area, controllable particle size and the like, can be used for the separation and purification of biological molecules such as fillers, proteins, amino acids and the like, the sustained release of drugs, the diagnosis of diseases, high-added-value products such as novel ceramic materials and raw materials of liquid crystal displays, can also be used as an ultrapure water treatment material in the industries such as medicines and semiconductors, and can also be used as an additive of bulk products such as coatings, paper surface coatings, cosmetics and the like. The particle size of the microspheres may not be uniform due to the influence of objective factors during the preparation process. The existing microsphere screening technology in the industry is not mature enough, and the ultrafiltration membrane with good screening effect is too expensive, so that a set of screening technology is urgently needed to be developed to obtain monodisperse microspheres with different particle sizes, and the use value of the polydisperse microspheres is improved.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the problems in the prior art, the invention provides the charged monodisperse polymer microsphere electrophoresis screening device which is low in price, simple and convenient in operation method, green, efficient and environment-friendly, and can screen monodisperse microspheres with different particle sizes from the monodisperse microspheres more simply and conveniently.
The technical scheme is as follows: the invention provides an electrophoresis screening device of charged polydisperse polymer microspheres, which comprises an emulsion pool with a vibration device and at least one group of conveying screening units, wherein the emulsion pool is communicated with the vibration device through a microsphere conveying pipeline; each group of conveying and screening units comprises a conveying pump and an electric field pool which are sequentially arranged along the flowing direction of the charged polydisperse microspheres to be screened; on the microsphere conveying pipeline, the emulsion pool and each group of conveying and screening units are sequentially arranged along the flowing direction of charged polydisperse microspheres to be screened; and the emulsion pool is communicated with the electric field pools in the last group of conveying and screening units through the microsphere conveying pipeline.
Preferably, after each electric field cell is electrified, the field intensity of the formed adsorption electric field is the same. If the field intensity of the adsorption electric field in each electric field pool is the same, the electric fields sequentially arranged on the flow path of the microspheres are used for adsorbing the microspheres in the same particle size range, and the later electric field pool is used for adsorbing the rest microspheres in the former electric field pool, so that the microspheres in the same particle size range can be completely adsorbed as much as possible.
Preferably, after each electric field cell is electrified, the field intensity of the adsorption electric field formed along the flow direction of the charged polydisperse microspheres to be screened is sequentially increased. If the field intensity of the adsorption electric field in each electric field pool is increased in sequence, the field intensity of the electric field pool in the front is smaller and is used for adsorbing the microspheres with larger particle sizes in the polydisperse microspheres, and the field intensity of the electric field pool is larger towards the rear, and is used for adsorbing the microspheres with smaller particle sizes in the polydisperse microspheres, so that the microspheres with different particle size ranges in the polydisperse microspheres can be separated out respectively.
Further, the conveying and screening units are divided into two groups; the first group comprises a first delivery pump and a first electric field pool, the second group comprises a second delivery pump and a second electric field pool, and the emulsion pool, the first delivery pump, the first electric field pool, the second delivery pump and the second electric field pool are arranged on the microsphere delivery pipeline in sequence.
Further, the emulsion pool is communicated with the second electric field pool through the microsphere conveying pipeline.
Further, a third delivery pump is further arranged on the microsphere delivery pipeline between the emulsion pool and the second electric field pool.
Preferably, the third delivery pump is a peristaltic pump.
Preferably, the electrode plate in each electric field cell is detachably mounted. The electrode plates in each electric field pool are detachably mounted, so that microspheres adsorbed and screened on the electrode plates can be conveniently collected.
Preferably, the vibration device is an ultrasonic vibration device. The ultrasonic vibration device is arranged on the emulsion pool, so that the charged polydispersed microspheres to be screened can be vibrated to an even state, namely, the microspheres with the particle sizes are evenly dispersed, and the subsequent efficient screening of each electric field pool is facilitated.
Preferably, the delivery pump is a peristaltic pump.
Has the advantages that: in the invention, firstly, the polydisperse microspheres to be screened are placed in emulsion pools, after the microsphere emulsion is uniformly vibrated by a vibrating device, voltage is applied to each electric field pool to form an adsorption electric field, the microsphere emulsion in each emulsion pool is sequentially conveyed through each electric field pool by a conveying pump, and when flowing through each electric field pool, the adsorption electric field formed in each electric field pool adsorbs the microspheres with the particle size range matched with the field intensity in the microsphere emulsion; if the field intensity of the adsorption electric field in each electric field pool is the same, the particle size of the microspheres adsorbed in each electric field pool is the same, and if the field intensity of the adsorption electric field in each electric field pool is increased in sequence, the particle size of the microspheres adsorbed in each electric field pool is reduced in sequence.
After the emulsion pool is communicated with the last electric field pool, the polydisperse microspheres screened by the last electric field pool can be circulated into the first electric field pool again, and then are screened for the second time by each electric field pool again in sequence, so that microspheres with smaller particle size can be screened conveniently, or more microspheres with the same particle size range remained by the previous screening can be screened conveniently; therefore, the method can be repeatedly circulated until microspheres with all particle sizes are screened out, and is convenient and efficient.
The device can realize simple and effective screening of the polydisperse microspheres by a physical method.
When the device is used, firstly, the charged polydisperse microspheres to be screened are uniformly distributed and flow, then, each electric field pool is electrified, an adsorption electric field is formed in each electric field pool, and if microspheres with the same particle size range are adsorbed in each electric field pool, the same voltage is applied to each electric field pool to form an adsorption electric field with the same field intensity; if want to adsorb the microballon that the particle diameter scope is different in each electric field pond, then according to the precedence order of microballon flow direction, form the absorption electric field that field intensity increases in proper order in each electric field pond in proper order, like this, adsorb the great microballon of particle diameter in the less electric field pond of preceding absorption electric field, adsorb the less microballon of particle diameter in the great electric field pond of later absorption electric field, treat to adsorb after stable, the outage, take out the plate electrode in each electric field pond and just can collect the microballon that adsorbs on the plate electrode, realize the screening of polydisperse microballon. The method is a physical screening method and has the advantages of being green, efficient and environment-friendly.
Drawings
FIG. 1 is a schematic structural diagram of an electrophoretic screening device with charged polydisperse polymeric microspheres according to embodiments 1 to 3;
FIG. 2 is a diagram showing large-particle-size polymer microspheres having a particle size of about 2700nm obtained by sieving in embodiments 1 to 3, and their characterization data;
FIG. 3 shows the medium-sized polymer microspheres with a particle size of about 800nm obtained by screening in embodiments 2 and 3 and the characterization data thereof;
FIG. 4 shows the data of the polymer microspheres with small particle size of about 300nm obtained by sieving in embodiment 3.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
Embodiment 1:
the embodiment provides an electrophoresis screening device for charged polydisperse polymer microspheres, which mainly comprises an emulsion tank and two groups of conveying and screening units, wherein the emulsion tank is communicated with the ultrasonic vibration device through a microsphere conveying pipeline, and the two groups of conveying and screening units are arranged on the emulsion tank. The first group of conveying and screening units comprise a first conveying pump and a first electric field pool, the second group of conveying and screening units comprise a second conveying pump and a second electric field pool, and the emulsion pool, the first conveying pump, the first electric field pool, the second conveying pump and the second electric field pool are sequentially arranged on the microsphere conveying pipeline along the flowing direction of the charged polydispersed microspheres to be screened. The second electric field pool is communicated with the emulsion pool through a microsphere conveying pipeline, a third conveying pump can be further installed on the microsphere conveying pipeline between the emulsion pool and the second electric field pool, and a peristaltic pump is preferably selected as the third conveying pump. The electrode plates in the first electric field pool and the second electric field pool can be detached, and the first conveying pump and the second conveying pump are peristaltic pumps.
The device is used for screening charged polydisperse polymer microspheres, and the screening method comprises the following steps:
(1) selecting materials: selecting charged polydisperse polymer microsphere emulsion with the particle size range of 200 nm-5 um.
(2) Feeding: and putting the charged polydisperse polymer microsphere emulsion into an emulsion pool.
(3) Electrifying: and respectively electrifying the electrode plates in the first electric field pool and the second electric field pool, wherein the voltage in the first electric field pool and the voltage in the second electric field pool are both 5V.
(4) Starting a power device: starting an ultrasonic vibration device (with the frequency of 28 kHz) to enable microspheres in the microsphere emulsion in the emulsion pool to be uniformly distributed, starting a first delivery pump and a second delivery pump (with the rotating speed of 60 rpm) to enable the microspheres to flow in a microsphere delivery pipeline, and enabling an electrode plate in a first electric field pool to adsorb the microspheres with the particle size of about 2700nm when the microsphere emulsion flows through the first electric field pool; then the microsphere emulsion flows through a second electric field tank, and electrode plates in the second electric field tank adsorb the residual microspheres with the particle size of about 2700 nm.
(5) Collecting: and turning off all power supplies, and respectively collecting microspheres adsorbed on the electrode plates in the first electric field pool and the second electric field pool, wherein the particle size of the microspheres is about 2700 nm.
Through the primary screening, the monodisperse microsphere with the particle size of about 2700nm (shown in figure 2) can be obtained, and the screening effect is good.
(6) Secondary electrification: electrifying the electrode plates in the first electric field pool and the second electric field pool respectively, wherein the voltage in the first electric field pool and the voltage in the second electric field pool are both 5V;
(7) and (4) repeating the step (4).
(8) Repeating the step (5).
Through the secondary screening, more uniform monodisperse microspheres with the particle size of 2700nm (as shown in figure 2) are obtained, and the screening effect is better.
Embodiment 2:
the device in this embodiment is completely the same as the screening device in embodiment 1, and is not described herein.
The device can be used for screening charged polydisperse polymer microspheres, and the screening method comprises the following steps:
(1) selecting materials: selecting charged polydisperse polymer microsphere emulsion with the particle size range of 200 nm-5 um.
(2) Feeding: and putting the charged polydisperse polymer microsphere emulsion into an emulsion pool.
(3) Electrifying: and (3) electrifying the electrode plates in the first electric field pool and the second electric field pool respectively, wherein the voltage of the first electric field pool is 5V, and the voltage of the second electric field pool is 25V.
(4) Starting a power device: starting an ultrasonic vibration device (with the frequency of 64 kHz) to uniformly distribute microspheres in the microsphere emulsion, starting a first delivery pump and a second delivery pump (with the rotating speed of 180 rpm) to enable the microspheres to flow in a microsphere delivery pipeline, and enabling an electrode plate in a first electric field tank to adsorb the microspheres with the particle size of about 2700nm when the microsphere emulsion flows through the first electric field tank; then the microsphere emulsion flows through a second electric field tank, and an electrode plate in the second electric field tank adsorbs microspheres with the particle size of about 800nm in the microsphere emulsion.
(5) Collecting: and turning off all power supplies, and respectively collecting microspheres adsorbed on the electrode plates in the first electric field pool and microspheres adsorbed on the electrode plates in the second electric field pool, wherein the particle size of the microspheres is about 2700nm and the particle size of the microspheres is about 800 nm.
Through the primary screening, two types of monodisperse microspheres with uniform grain sizes of 2700nm (shown in figure 2) and 800nm (shown in figure 3) can be obtained, and the screening effect is good.
(6) Secondary electrification: and electrifying the electrode plates in the first electric field pool and the second electric field pool respectively, wherein the voltage in the first electric field pool is 5V, and the voltage in the second electric field pool is 25V.
(7) And (4) repeating the step (4).
(8) Repeating the step (5).
Through the secondary screening, two types of monodisperse microspheres with more uniform particle sizes of 2700nm (shown in figure 2) and 800nm (shown in figure 3) are obtained.
Embodiment 3:
the device in this embodiment is completely the same as the screening device in embodiment 1, and is not described herein.
The device can be used for screening charged polydisperse polymer microspheres, and the screening method comprises the following steps:
steps (1) to (5) are completely the same as embodiment 2, and are not described herein.
After the power is cut off, microspheres with the grain size of about 2700nm (as shown in figure 2) adsorbed on the electrode plate in the first electric field pool and microspheres with the grain size of about 800nm (as shown in figure 3) adsorbed on the electrode plate in the second electric field pool are respectively collected, the method further comprises the following steps:
(6) secondary electrification: and electrifying the electrode plates in the first electric field pool and the second electric field pool respectively, wherein the voltage in the first electric field pool is 25V, and the voltage in the second electric field pool is 50V.
(7) Starting an ultrasonic vibration device (with the frequency of 64 kHz) to uniformly distribute microspheres in the microsphere emulsion, starting a first delivery pump and a second delivery pump (with the rotating speed of 180 rpm) to enable the microspheres to flow in a microsphere delivery pipeline, and when the microsphere emulsion flows through a first electric field tank, adsorbing and screening the remaining microspheres with the particle size of about 800nm by an electrode plate in the first electric field tank for one time; then the microsphere emulsion flows through a second electric field tank, and an electrode plate in the second electric field tank adsorbs microspheres with the particle size of about 300nm in the microsphere emulsion.
(8) Collecting: and turning off all power supplies, and respectively collecting microspheres with the particle size of about 800nm adsorbed on the electrode plates in the first electric field pool and microspheres with the particle size of about 300nm adsorbed on the electrode plates in the second electric field pool.
Through the screening, the microspheres with the residual particle size of 800nm (shown in figure 3) after the primary screening can be further screened, the monodisperse microspheres with the smaller particle size of about 300nm (shown in figure 4) can be obtained, and the monodisperse microspheres with three particle size ranges can be obtained through the secondary screening, so that the screening effect is better.
The above embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (10)
1. The utility model provides an electrophoresis screening plant of polydisperse polymer microballon of charged which characterized in that: the device comprises an emulsion pool with a vibration device and at least one group of conveying and screening units which are communicated through a microsphere conveying pipeline; each group of conveying and screening units comprises a conveying pump and an electric field pool which are sequentially arranged along the flowing direction of the charged polydisperse microspheres to be screened; on the microsphere conveying pipeline, the emulsion pool and each group of conveying and screening units are sequentially arranged along the flowing direction of charged polydisperse microspheres to be screened;
and the emulsion pool is communicated with the electric field pools in the last group of conveying and screening units through the microsphere conveying pipeline.
2. The device for electrophoretic separation of charged polydisperse polymeric microspheres of claim 1, further comprising: after the electric field cells are electrified, the formed adsorption electric fields have the same field intensity.
3. The device for electrophoretic separation of charged polydisperse polymeric microspheres of claim 1, further comprising: and after each electric field pool is electrified, the field intensity of an adsorption electric field formed along the flowing direction of the charged polydisperse microspheres to be screened is increased in sequence.
4. The device for electrophoretic separation of charged polydisperse polymeric microspheres of claim 1, further comprising: the conveying and screening units are divided into two groups; the first group comprises a first delivery pump and a first electric field pool, the second group comprises a second delivery pump and a second electric field pool, and the emulsion pool, the first delivery pump, the first electric field pool, the second delivery pump and the second electric field pool are arranged on the microsphere delivery pipeline in sequence.
5. The device for electrophoretically sieving a charged polydisperse polymeric microsphere of claim 2, further comprising: the emulsion pool is communicated with the second electric field pool through the microsphere conveying pipeline.
6. The device for electrophoretic separation of charged polydisperse polymeric microspheres of claim 5, further comprising: and a third delivery pump is also arranged on the microsphere delivery pipeline between the emulsion pool and the second electric field pool.
7. The device of claim 6, wherein the charged polydisperse polymeric microspheres of an electrophoretic screening device are: the third delivery pump is a peristaltic pump.
8. An electrophoretic screening device for charged polydisperse polymeric microspheres as claimed in any one of claims 1 to 7, wherein: the electrode plates in each electric field pool are detachably arranged.
9. An electrophoretic screening device for charged polydisperse polymeric microspheres as claimed in any one of claims 1 to 7, wherein: the vibration device is an ultrasonic vibration device.
10. An electrophoretic screening device for charged polydisperse polymeric microspheres as claimed in any one of claims 1 to 7, wherein: the first conveying pump and the second conveying pump are both peristaltic pumps.
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