CN113136322B - Air phage and mixed bacteria pre-killing system for fermentation - Google Patents
Air phage and mixed bacteria pre-killing system for fermentation Download PDFInfo
- Publication number
- CN113136322B CN113136322B CN202110526367.7A CN202110526367A CN113136322B CN 113136322 B CN113136322 B CN 113136322B CN 202110526367 A CN202110526367 A CN 202110526367A CN 113136322 B CN113136322 B CN 113136322B
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- China
- Prior art keywords
- air
- inlet
- outlet
- sterilizing liquid
- sterilizer
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- 238000000855 fermentation Methods 0.000 title claims abstract description 16
- 230000004151 fermentation Effects 0.000 title claims abstract description 16
- 241000894006 Bacteria Species 0.000 title claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 87
- 230000001954 sterilising effect Effects 0.000 claims abstract description 66
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000835 fiber Substances 0.000 claims abstract description 13
- 244000005700 microbiome Species 0.000 claims abstract description 9
- 238000010992 reflux Methods 0.000 claims abstract description 9
- 239000007791 liquid phase Substances 0.000 claims abstract description 4
- 239000012071 phase Substances 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 241001515965 unidentified phage Species 0.000 claims description 4
- 230000007797 corrosion Effects 0.000 claims description 2
- 238000005260 corrosion Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 229910052755 nonmetal Inorganic materials 0.000 claims description 2
- 238000009991 scouring Methods 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 claims 1
- 230000002458 infectious effect Effects 0.000 claims 1
- 238000001914 filtration Methods 0.000 abstract description 7
- 230000000813 microbial effect Effects 0.000 abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 4
- 238000001704 evaporation Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
Abstract
The invention relates to air sterilization equipment, in particular to an air phage and mixed bacteria pre-killing system for fermentation. The sterilizing device comprises an air inlet line, wherein the air inlet line is connected with a sterilizer inlet, a sterilizer outlet is connected with a liquid separator inlet, a gas phase outlet of the liquid separator is connected with an air discharge line, a liquid phase outlet of the liquid separator is connected with a sterilizing liquid tank inlet, a sterilizing liquid tank outlet is connected with a circulating pump inlet, a circulating pump outlet is connected with a filter inlet, the filter outlet is divided into two branches, one branch is connected to the sterilizer through a sterilizing liquid inlet pipe, the other branch is connected to a water remover top inlet, a water remover outlet is connected with a reflux pump inlet, and a reflux pump outlet is connected with a sterilizing liquid tank inlet; wherein, the inner wall of the sterilizer is connected with bionic fiber rods in a staggered way. The invention has simple structure and reasonable design, and can remove and kill most of harmful microorganisms in advance before the fermentation air enters the filtering system, and adsorb harmful components in part of the air, thereby purifying the air and meeting the aseptic requirement of microbial fermentation.
Description
Technical Field
The invention relates to air sterilization equipment, in particular to an air phage and mixed bacteria pre-killing system for fermentation.
Background
Some microbial fermentations require the use of sterile air, which is conventionally obtained by multistage filtration, but due to the complexity of practical operations, air filtration sterilization is often disabled, so that improper active bacteria or even phages are introduced into the sterile environment, which causes significant losses in industrial production.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an air phage and mixed bacteria pre-killing system for fermentation, which has a simple structure and reasonable design, and is used for pre-removing and killing most of harmful microorganisms before the fermentation air enters a filtering system according to the principle that harmful substances in the air are blocked by biological respiratory tracts, and adsorbing harmful components in part of the air, so that the air is purified and the sterile requirement of microbial fermentation is met.
In order to achieve the above object, the technical scheme of the present invention is as follows:
the air phage and bacteria pre-killing system for fermentation comprises an air inlet line, wherein the air inlet line is connected with a sterilizer inlet, a sterilizer outlet is connected with a liquid separator inlet, a gas phase outlet of the liquid separator is connected with an air discharge line, a liquid phase outlet of the liquid separator is connected with a sterilizing liquid tank inlet, a sterilizing liquid tank outlet is connected with a circulating pump inlet, a circulating pump outlet is connected with a filter inlet, the filter outlet is divided into two branches, one branch is connected to the sterilizer through a sterilizing liquid inlet pipe, the other branch is connected to a top inlet of a dehydrator, the dehydrator outlet is connected with a reflux pump inlet, and the reflux pump outlet is connected with the sterilizing liquid tank inlet;
wherein, the inner wall of the sterilizer is connected with bionic fiber rods in a staggered way, the top of the sterilizer is connected with a cleaning water pipeline, and the bottom of the sterilizer is provided with a liquid outlet. The bionic fiber rod is made of metal or nonmetal materials with elasticity, corrosion resistance and airflow scouring resistance.
Wherein:
preferably, an air flow meter is provided on the air inlet line.
Preferably, a sterilizing liquid heater is arranged in the sterilizing liquid tank and is used for heating the sterilizing liquid.
Preferably, the top of the dehydrator is provided with a liquid filling port.
Preferably, the top of the dehydrator is provided with an exhaust port.
Preferably, the lower part of the dehydrator is connected with a steam heater.
Working principle and process:
during operation, air passes through the sterilizer under the control of the air flowmeter, and substances such as particulate matters, microorganisms, bacteriophage and the like in the air collide with the bionic fiber rod under the action of the bionic interception system, so that alkaline and viscous sterilizing liquid on the bionic fiber is sucked in a sticking manner. The air after being stuck and absorbed entrains the sterilizing liquid into the liquid separator, the sterilized air is discharged out of the system through the air discharge line, and the sterilizing liquid enters the sterilizing liquid tank.
The sterilizing liquid enters a sterilizing liquid tank for heating, after the temperature is raised to a set temperature, the sterilizing liquid enters a filter for filtering under the conveying of a circulating pump, a part of the sterilizing liquid enters a sterilizer for sterilizing and adsorbing microorganism phage, and a part of the sterilizing liquid enters a dehydrator for evaporating water in air adsorbed by the sterilizing liquid.
Returning the sterilized solution after water removal to the sterilized solution tank to balance the concentration of the sterilized solution.
The sterilizing liquid discharged from the liquid discharge port can be added into the dehydrator through the liquid feeding port to be dehydrated and then recycled into the system for removal.
The control links can be automatically controlled.
Compared with the prior art, the invention has the following beneficial effects:
the invention has simple structure and reasonable design and imitates living thingsThe principle of blocking harmful substances in the air by the respiratory tract is to remove and kill most of harmful microorganisms in advance before the fermentation air enters the filtering system, and adsorb harmful components in part of the air, so that the air is purified and the sterile requirement of microbial fermentation is met. The air after sterilization can reach the air cleanliness grade of 10 ten thousand clean rooms. The particle number is more than or equal to 0.5 mu m and less than 35 ten thousand, the particle number is more than or equal to 5 mu m and less than 2 ten thousand, and the number of planktonic bacteria is less than 500 per m 3 。
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
in the figure: 1. an air inlet line; 2. a sterilizer; 3. cleaning a water line; 4. a bionic fiber rod; 5. a liquid outlet; 6. a sterilizing liquid inlet pipe; 7. an air discharge line; 8. a knockout; 9. a sterilizing liquid tank; 10. a sterilizing liquid heater; 11. a circulation pump; 12. a filter; 13. a reflux pump; 14. a water remover; 15. a steam heater; 16. a liquid adding port; 17. an exhaust port; 18. an air flow meter.
Detailed Description
The invention is further described in connection with the following detailed description.
Example 1
As shown in figure 1, the system for pre-killing the air bacteriophage and the mixed bacteria for fermentation comprises an air inlet line 1, wherein the air inlet line 1 is connected with an inlet of a sterilizer 2, an outlet of the sterilizer 2 is connected with an inlet of a liquid separator 8, a gas phase outlet of the liquid separator 8 is connected with an air discharge line 7, a liquid phase outlet of the liquid separator 8 is connected with an inlet of a sterilizing liquid tank 9, an outlet of the sterilizing liquid tank 9 is connected with an inlet of a circulating pump 11, an outlet of the circulating pump 11 is connected with an inlet of a filter 12, an outlet of the filter 12 is divided into two branches, one branch is connected to the sterilizer 2 through a sterilizing liquid inlet pipe 6, the other branch is connected with an inlet at the top of a dehydrator 14, an outlet of the dehydrator 14 is connected with an inlet of a reflux pump 13, and an outlet of the reflux pump 13 is connected with an inlet of the sterilizing liquid tank 9;
wherein, the inner wall of the sterilizer 2 is connected with bionic fiber rods 4 in a staggered way, the top of the sterilizer 2 is connected with a cleaning water pipeline 3, and the bottom of the sterilizer 2 is provided with a liquid outlet 5.
An air flow meter 18 is provided on the air inlet line 1.
A sterilizing liquid heater 10 is arranged in the sterilizing liquid tank 9 and is used for heating the sterilizing liquid.
A liquid filling port 16 is arranged at the top of the dehydrator 14.
The top of the water remover 14 is provided with an exhaust port 17.
The lower part of the dehydrator 14 is connected with a steam heater 15.
During operation, air passes through the sterilizer 2 under the control of the air flowmeter 18, and substances such as particulate matters, microorganisms, bacteriophage and the like in the air collide with the bionic fiber rod 4 under the action of the bionic interception system, so that alkaline and viscous sterilizing liquid on the bionic fiber is sucked in a sticking manner. The air after being stuck and absorbed entrains sterilizing liquid into a liquid separator 8, the sterilized air is discharged out of the system through an air discharge line 7, and the sterilizing liquid enters a sterilizing liquid tank 9.
The sterilizing liquid enters the sterilizing liquid tank 9 for heating, after the temperature is raised to a set temperature, the sterilizing liquid enters the filter 12 for filtering under the conveying of the circulating pump 11, part of the sterilizing liquid enters the sterilizer 2 for sterilizing and adsorbing microorganism phage, and part of the sterilizing liquid enters the dehydrator 14 for evaporating water in the air adsorbed by the sterilizing liquid.
The sterilized solution after water removal returns to the sterilized solution tank 9 to balance the concentration of the sterilized solution.
The sterilizing liquid discharged from the liquid outlet 5 can be added into the dehydrator 14 through the liquid inlet 16 to be dehydrated and then recycled into the system.
Of course, the above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (2)
1. An air phage and mixed bacteria pre-killing system for fermentation is characterized in that: the sterilizing device comprises an air inlet line (1), wherein the air inlet line (1) is connected with an inlet of a sterilizer (2), an outlet of the sterilizer (2) is connected with an inlet of a liquid separator (8), a gas phase outlet of the liquid separator (8) is connected with an air discharge line (7), a liquid phase outlet of the liquid separator (8) is connected with an inlet of a sterilizing liquid tank (9), an outlet of the sterilizing liquid tank (9) is connected with an inlet of a circulating pump (11), an outlet of the circulating pump (11) is connected with an inlet of a filter (12), an outlet of the filter (12) is divided into two branches, one branch is connected to the sterilizer (2) through a sterilizing liquid inlet pipe (6), the other branch is connected to an inlet at the top of a dehydrator (14), an outlet of the dehydrator (14) is connected with an inlet of a reflux pump (13), and an outlet of the reflux pump (13) is connected with an inlet of the sterilizing liquid tank (9);
wherein bionic fiber rods (4) are connected to the inner wall of the sterilizer (2) in a staggered manner, the top of the sterilizer (2) is connected with a cleaning water pipeline (3), and a liquid outlet (5) is arranged at the bottom of the sterilizer (2); the bionic fiber rod is made of elastic, corrosion-resistant, airflow scouring-resistant metal or nonmetal materials;
a sterilizing liquid heater (10) is arranged in the sterilizing liquid tank (9);
a liquid adding port (16) is arranged at the top of the dehydrator (14);
the lower part of the dehydrator (14) is connected with a steam heater (15);
working principle and process:
during operation, air passes through the sterilizer under the control of the air flowmeter, and particulate matters, microorganism phage and bionic fiber rods in the air are impacted under the action of the bionic interception system, so that alkaline and viscous sterilizing liquid on the bionic fibers are sucked in a sticking manner; the air after being stuck and absorbed entrains sterilizing liquid into a liquid separator, the sterilized air is discharged out of the system through an air discharge line, and the sterilizing liquid enters a sterilizing liquid tank;
the sterilizing liquid enters a sterilizing liquid tank to heat up, after the temperature is raised to a set temperature, the sterilizing liquid enters a filter to be filtered under the conveying of a circulating pump,
part of the water enters a sterilizer to sterilize and adsorb microorganism phage, and part of the water enters a dehydrator to evaporate water in the air adsorbed by the sterilizing liquid;
returning the sterilized solution after water removal to a sterilized solution tank to balance the concentration of the sterilized solution;
the sterilizing liquid discharged from the liquid discharge port can be added into the dehydrator through the liquid feeding port to be dehydrated and then recycled into the system for removal;
an air flow meter (18) is arranged on the air inlet line (1).
2. The system for pre-killing bacteriophage and infectious microbe for fermentation according to claim 1, wherein the system comprises: an exhaust port (17) is arranged at the top of the dehydrator (14).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110526367.7A CN113136322B (en) | 2021-05-14 | 2021-05-14 | Air phage and mixed bacteria pre-killing system for fermentation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202110526367.7A CN113136322B (en) | 2021-05-14 | 2021-05-14 | Air phage and mixed bacteria pre-killing system for fermentation |
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CN113136322A CN113136322A (en) | 2021-07-20 |
CN113136322B true CN113136322B (en) | 2023-12-08 |
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CN202110526367.7A Active CN113136322B (en) | 2021-05-14 | 2021-05-14 | Air phage and mixed bacteria pre-killing system for fermentation |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6261614A (en) * | 1985-09-10 | 1987-03-18 | Aoki Kensetsu:Kk | Sterilizing filter |
CN101947328A (en) * | 2010-09-03 | 2011-01-19 | 方民 | Method for performing strengthened sterilization on biological fermentation air supply and exhaust and strengthening sterilizers |
CN104324512A (en) * | 2014-11-07 | 2015-02-04 | 徐建涛 | Heat pump evaporating unit |
CN107496968A (en) * | 2017-09-07 | 2017-12-22 | 凯莱英医药集团(天津)股份有限公司 | Remove the method and system of fermentation compressed air pnagus medius |
CN107588487A (en) * | 2017-10-19 | 2018-01-16 | 天津雾净环保科技有限公司 | A kind of air-conditioning system of efficient sterilizing |
JP2020067245A (en) * | 2018-10-25 | 2020-04-30 | 株式会社ピーズガード | Air supply device |
CN213159867U (en) * | 2020-07-02 | 2021-05-11 | 四川海恩贝生物科技有限公司 | Abamectin sterile air filtering structure for dogs |
-
2021
- 2021-05-14 CN CN202110526367.7A patent/CN113136322B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6261614A (en) * | 1985-09-10 | 1987-03-18 | Aoki Kensetsu:Kk | Sterilizing filter |
CN101947328A (en) * | 2010-09-03 | 2011-01-19 | 方民 | Method for performing strengthened sterilization on biological fermentation air supply and exhaust and strengthening sterilizers |
CN104324512A (en) * | 2014-11-07 | 2015-02-04 | 徐建涛 | Heat pump evaporating unit |
CN107496968A (en) * | 2017-09-07 | 2017-12-22 | 凯莱英医药集团(天津)股份有限公司 | Remove the method and system of fermentation compressed air pnagus medius |
CN107588487A (en) * | 2017-10-19 | 2018-01-16 | 天津雾净环保科技有限公司 | A kind of air-conditioning system of efficient sterilizing |
JP2020067245A (en) * | 2018-10-25 | 2020-04-30 | 株式会社ピーズガード | Air supply device |
CN213159867U (en) * | 2020-07-02 | 2021-05-11 | 四川海恩贝生物科技有限公司 | Abamectin sterile air filtering structure for dogs |
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CN113136322A (en) | 2021-07-20 |
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