CN113117151A - Bone tissue engineering scaffold material and preparation method thereof - Google Patents
Bone tissue engineering scaffold material and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of high-tech materials, and particularly relates to a bone tissue engineering scaffold material and a preparation method thereof. The material takes polyglycolide-polycaprolactone two blocks as a polymer main material, and takes mesoporous bioactive glass powder as an inorganic bioactive component; the mesoporous bioactive glass powder is uniformly dispersed in the stent material. The scaffold material has the pore diameter and porosity which accord with those of the scaffold material for tissue engineering, and has good formability, bone induction and conductivity; the bone tissue engineering scaffold material overcomes the defects of uncontrollable degradation period, poor bone inductivity and conductivity, high preparation cost and the like of the conventional scaffold material, has good bone formation promoting effect, and is suitable for repairing various bone defects. In addition, the preparation method of the bone tissue engineering scaffold material is simple, has low cost and is suitable for large-scale production.
Description
Technical Field
The invention belongs to the technical field of high-tech materials, and particularly relates to a bone tissue engineering scaffold material and a preparation method thereof.
Background
Large bone defects caused by trauma, infection, tumor, etc. are common conditions in orthopedics clinic. Bone defect surgery has become second only to blood transfusion, second to human tissue transplantation, in terms of quantity, and the clinical demand is enormous. Conventional bone defect repair methods include: autologous bone transplantation, allogeneic bone transplantation and xenogeneic bone transplantation, but the methods have great disadvantages and greatly limit the clinical application of the methods. At present, the bioactive artificial bone which adopts a tissue engineering method to construct composite seed cells, scaffold materials and growth factors is considered to be one of the most effective methods for repairing bone defects and reconstructing bones in the future.
The scaffold material is used as a framework for bone tissue regeneration, is one of central links of bone tissue engineering research, and directly influences the survival, migration, proliferation and metabolism of seed cells. An ideal stent material would have: the biological compatibility is good, the biological compatibility is non-toxic, the biological agent is not abnormal, and the biological agent can be safely used for human bodies; good plastic formability and certain mechanical strength; thirdly, a three-dimensional porous structure with proper pore diameter (200-400 mu m) and high porosity (preferably more than 85 percent); degradability and controllability of degradation rate; no antigen effect; sixthly, a good material/cell interface can promote the adhesion and proliferation of cells; the source is not limited, and the sterilization and the transportation are easy. At present, the bone tissue engineering scaffold material mainly comprises a natural scaffold material, an artificially synthesized scaffold material and a composite scaffold material.
The natural scaffold material mainly comprises natural high molecular materials (chitosan, chitin, hyaluronic acid, collagen and the like) and natural inorganic materials (natural coral, deproteinized bones of human or animals and the like). The natural scaffold material is generally nontoxic, weak in antigenicity, good in hydrophilicity, biocompatibility and cell affinity, and the degradation product amino acid can be completely absorbed, but the defects are that the strength is poor, the degradation time cannot be accurately calculated, the mechanical and processing properties are poor, and the quality repeatability is poor.
The artificially synthesized scaffold material is extracellular matrix substitute synthesized by a physical and chemical method, and mainly synthesized by inorganic scaffold materials (hydroxyapatite, tricalcium phosphate and the like) and organic polymer scaffold materials (polylactic acid, polyglycolic acid and the like). The inorganic support material has good biocompatibility and osteoconductivity, and the high calcium ion layer and the slightly alkaline environment formed by the mild dissolution of the bioceramic can effectively promote the adhesion, proliferation and differentiation of osteocytes. But has the disadvantages of great brittleness, poor degradability, and fracture resistance and impact resistance which can not meet the requirements of artificial bones. The organic polymer scaffold material is easy to shape, has good cell compatibility and controllable degradability and absorptivity, and degradation products can be metabolized through a physiological metabolic pathway; the defects are that the hydrophilicity is insufficient, the cell adsorption force is weak, the degradation product can lead the local microenvironment to be acidic, the adverse effect is generated on the tissues, the mechanical strength is insufficient, and the like.
The composite scaffold material is prepared by combining the pure natural material and the artificial synthetic material, has excellent and short properties, better meets the requirements of bone tissue engineering, and can be better applied to clinical bone repair. The composite material integrates the advantages of various materials, not only can ensure that the material has enough strength, but also can effectively combine seed cells and growth factors, simultaneously has proper degradation rate, can be matched with bone tissues to be reconstructed, and can increase the bone transferability and the bone inductivity of the scaffold material. In recent years, the research focus of bone tissue engineering scaffold materials is mainly focused on the research of composite materials. The composite material mainly comprises the composition of a biological ceramic material and a natural polymer material, the composition of the biological ceramic material and an artificial synthetic material, the composition of the biological ceramic material and the like. Although the composite scaffold material has good effects in various bone repairs, no bone tissue engineering scaffold material can solve all the problems in bone repair at present, and more problems still exist in various bone repairs, such as the problem of matching of the degradation rate of the scaffold material with the bone formation rate. Polylactic acid-Polycaprolactone (PLGA) is an organic polymer material approved by FDA to be used clinically, so that a large amount of composite scaffold materials taking PLGA as an organic main body are prepared and applied to various bone repair, but the degradation performance and the osteogenesis performance are still to be further improved. PLGA is degradable and adjustable, but the degradation period is different from half a year to several years, after the composite stent material is compounded with other materials, the degradation rate of the composite stent material can be evaluated more accurately only by considering the self-attribute of the PLGA and the influence of other materials on the degradation of the PLGA, and further the matching with the osteogenesis rate can be realized by comprehensively regulating and controlling various properties of the stent material. Therefore, at present, there is still a need to develop a new scaffold material for bone tissue engineering and a preparation technology thereof, and particularly, to prepare a scaffold material more suitable for various clinical bone repair aiming at the key detail problems existing in various bone defect repairs.
Mesoporous bioactive glass (MGB) refers to a class of porous bioactive glass materials with pore sizes between 2-50 nm. Compared with the traditional non-mesoporous bioactive glass, the MGB has higher specific surface area and pore volume and shows better osteogenesis performance, and the MBG is an inorganic material, can obviously improve the hydrophobic performance of the polymer after being compounded with the polymer and improve the biocompatibility of the polymer, and in addition, the degraded product mainly exists in an ion form, can be metabolized by a human body and has no serious toxic effect on the human body, so the MBG is widely used as an inorganic component for preparing the composite scaffold material.
Disclosure of Invention
The invention mainly aims to provide a bone tissue engineering scaffold material and a preparation method thereof, and the scaffold material has the advantages of aperture and porosity which accord with the scaffold material for tissue engineering, good formability, bone induction and conductivity; the bone tissue engineering scaffold material overcomes the defects of uncontrollable degradation period, poor bone inductivity and conductivity, high preparation cost and the like of the conventional scaffold material, has good bone formation promoting effect, and is suitable for repairing various bone defects. In addition, the preparation method of the bone tissue engineering scaffold material is simple, has low cost and is suitable for large-scale production.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a bone tissue engineering scaffold material, which takes polyglycolide-polycaprolactone two blocks as a polymer main material and takes mesoporous bioactive glass powder as an inorganic bioactive component; the mesoporous bioactive glass powder is uniformly dispersed in the stent material.
Preferably, the pore diameter of the bone tissue engineering scaffold material is 300-450 μm, and the porosity is 75-80%.
Preferably, the mesoporous bioactive glass powder accounts for 10-40% of the weight of the bone tissue engineering scaffold material.
Further preferably, the mesoporous bioactive glass powder is silicon-calcium-phosphorus mesoporous bioactive glass powder or vanadium-doped silicon-calcium-phosphorus mesoporous bioactive glass powder.
Preferably, SiO in the silicon-calcium-phosphorus mesoporous bioactive glass powder2、CaO、P2O5In a molar ratio of 50-100:15: 2.5; the macro morphology is a cluster or chain structure formed by bonding and twisting vermicular structures, the grain diameter of the vermicular particles is 0.5-2 mu m, and the specific surface area is more than 300m2The mesoporous aperture is larger than 6nm and the macroscopic particle size is smaller than 45 mu m.
Preferably, the preparation method of the silicon-calcium-phosphorus mesoporous bioactive glass powder comprises the following steps:
(1) ventilating 0.5-1.0mol/L hydrochloric acid at room temperature, measuring a certain amount of hydrochloric acid, and adding into anhydrous ethanol to obtain a mixed solution;
(2) firstly, dissolving a template agent polyethylene oxide-polypropylene oxide-polyethylene oxide triblock copolymer (P123) in the mixed solution obtained in the step (1), stirring at normal temperature until the P123 is completely dissolved, and then adding Ca (NO)3)2·4H2O, after the mixture is completely dissolved, ethyl Triphosphate (TEP) and Tetraethoxysilane (TEOS) are sequentially added into the mixed solution in a dropwise mannerWherein the dropping speed of TEOS and TEP is 15-20 drops/min;
(3) after all the sol is added, continuously stirring for 24-48h at room temperature to obtain sol solution, transferring the obtained sol solution to a culture dish for induced evaporation, continuously inducing for 48-120h at room temperature to obtain xerogel, placing the xerogel in a high-temperature calcining box-type furnace, heating to 250-300 ℃ at a heating rate of 1-3 ℃/min, keeping the temperature for 2-3h, continuing to heat to 600 ℃ and 700 ℃, wherein the heating rate is 1-2 ℃/min, then preserving heat for 6-8h, keeping the whole calcining process in air atmosphere, closing the box furnace after calcining, naturally cooling to room temperature at room temperature, taking out the sample, finally grinding the obtained white powder without the template agent, sieving with a sieve of less than 325 meshes, thus obtaining the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing bone tissue engineering scaffold materials.
Further preferably, the template P123 used has a molecular weight MW of 5800Da, Ca (NO)3)2·4H2O, TEP, TEOS, and absolute ethanol analytically pure liquids were used, P123/absolute ethanol 1/15 (mass g/volume ml)
Preferably, the preparation method of the vanadium-doped silicon-calcium-phosphorus mesoporous bioactive glass powder specifically comprises the following steps:
1) preparing concentrated hydrochloric acid: preparing 1.6-2.0mol/L concentrated hydrochloric acid under the condition of ventilation room temperature;
2) dispersing the template agent: weighing the template agent, adding the template agent into the hydrochloric acid, and stirring at the stirring speed of 1000-3000r/min for 0.5-2 h at the temperature of 30-40 ℃ until the template agent is uniformly dispersed in the hydrochloric acid solution;
3) introduction of calcium, phosphorus, silicon and vanadium sources: under the condition of continuous stirring, firstly adding a vanadium source, adding a calcium source after the vanadium source is completely dissolved, adding a phosphorus source after the vanadium source is completely dissolved, and finally adding a silicon source, wherein the molar ratio of the silicon source to the calcium source to the phosphorus source to the vanadium source is 80-150:0.5-1:1-5: 5-15;
4) a hydrothermal process: after the silicon source of vanadium, calcium, phosphorus and silicon is added, continuously stirring the mixed system obtained in the step 3) for 12-24 hours at 35-38 ℃ to obtain a sol solution, then transferring the sol solution into a reaction kettle under the drainage of a glass rod, controlling the temperature to be 90-120 ℃, and carrying out hydrothermal reaction for 24-72 hours;
5) collecting mesoporous powder: after the reaction is finished, naturally cooling to room temperature, centrifuging the mixed system obtained in the step 4) at 7000r/min for 5-10min, pouring out supernatant, sequentially washing with distilled water and absolute ethyl alcohol for 2-5 times respectively, transferring the powder into a square porcelain boat, placing the square porcelain boat in an electric heating air blast drying oven, setting the temperature at 40-60 ℃, and drying for 12-24h to obtain mesoporous bioactive glass powder;
6) removing the template agent: placing the mesoporous bioactive glass powder obtained in the step 5) in a high-temperature box furnace, keeping the temperature for 2-3h when the temperature is raised to 250-300 ℃, continuing to raise the temperature to 600-700 ℃, keeping the temperature at the rate of 1-2 ℃/min, keeping the temperature for 6-8h, keeping the whole calcining process in the air atmosphere, closing the box furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, taking out a sample, and obtaining the white vanadium-doped calcium-silicon-phosphorus mesoporous bioactive glass powder without the template agent.
The invention also provides a preparation method of the bone tissue engineering scaffold material, which comprises the following steps:
dissolving polyglycolide-polycaprolactone in hexafluoroisopropanol, adding mesoporous bioactive glass powder, uniformly dispersing in the solution, and adding a pore-forming agent; granulating, molding, and filtering.
Preferably, the solid-to-liquid ratio of polyglycolide-polycaprolactone to hexafluoroisopropanol is 10-15% w/v; the mass ratio of the polyglycolide-polycaprolactone to the pore-foaming agent is 1: 6-7.
Preferably, the method for uniformly dispersing the mesoporous bioactive glass powder in the solution comprises the following steps: after the mesoporous bioactive glass powder is added into the solution, continuously stirring for 12-24h at 4000r/min of 2000-.
Preferably, the pore-foaming agent is sodium chloride, and the particle diameter of the sodium chloride particles is 300-450 μm.
Preferably, the granulation comprises the following steps: dropwise adding the solution added with the pore-foaming agent into absolute ethyl alcohol for granulation, wherein the dropwise adding speed is 20-30 drops/min, stirring is carried out simultaneously, and the stirring speed is 500-;
preferably, the press mold comprises the steps of: after granulation, continuously stirring for 30-50min at the stirring speed of 1000-2000r/min, pouring off the absolute ethyl alcohol, adding new absolute ethyl alcohol, continuously stirring for 1-5h at the same stirring speed, collecting precipitate, putting the precipitate into a mold, and pressing for 3-5min at the pressure of 8-12MPa to obtain a sample;
preferably, the leaching comprises the steps of: and drying the pressed sample for 12-24h under the ventilation condition at room temperature, then putting the sample into water at 4000-6000r/min, stirring and soaking for 72-120h, changing water once at intervals of 6-12h, observing the leaching condition of the scaffold at any time to adjust the stirring speed, and finally airing the leached scaffold material under the ventilation condition at room temperature to obtain the final scaffold material for bone tissue engineering.
Compared with the prior art, the invention has the following advantages:
polyethylene glycol lactone (PGA) is a polymer with a faster degradation rate than polylactic acid (PLLA); polycaprolactone (PCL) is a polymer with relatively slow degradation rate, so the PGA-PCL two-block polymer synthesized by taking PGA and PCL as raw materials has more controllable degradation period and wider degradation period range. The MBG/PGA-PCL composite scaffold material is prepared by combining MBG with excellent bone promotion performance and PGA-PCL with controllable degradation rate, and can realize better bone repair effect.
The scaffold material has the pore diameter and porosity which accord with those of the scaffold material for tissue engineering, and has good formability, bone induction and conductivity; the degradation period can be regulated, and the mechanical property, hydrophilicity and hydrophobicity, degradation rate and bone regeneration and repair capacity of the composite scaffold material are regulated and controlled by regulating the proportion of the mesoporous bioactive glass to the poly (caproic acid-polycaprolactone); the bone tissue engineering scaffold material overcomes the defects of uncontrollable degradation period, poor bone inductivity and conductivity, high preparation cost and the like of the conventional scaffold material, has good osteogenesis promoting effect, and is suitable for repairing various bone defects. The mesoporous bioactive glass/polyglycolide-polycaprolactone composite scaffold material has comprehensive physical and chemical properties and bone effect promotion, is used as a bone repair material in the field of bone tissue engineering, and has wide application prospect.
The preparation method of the bone tissue engineering scaffold material is simple, has low cost and is suitable for large-scale production.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a macroscopic morphology chart of the mesoporous bioactive glass prepared in example 1 and a mesoporous structure performance characterization chart thereof;
FIG. 2 is a macro-topography of MBG/PGA-PCL composite scaffold material prepared in example 2;
FIG. 3 is a graph showing the change in porosity of the MBG/PGA-PCL composite scaffold material prepared in example 3;
FIG. 4 is a MicroCT three-dimensional reconstruction map and a scanning electron microscope map of the MBG/PGA-PCL composite scaffold material prepared in example 4;
FIG. 5 is a graph of the distribution of Si, Ca and P elements on the scaffold material and an EDS energy spectrum on the MBG/PGA-PCL composite scaffold material prepared in example 5;
FIG. 6 is an in vivo degradation graph of the MBG/PGA-PCL composite scaffold material prepared in examples 4-6;
FIG. 7 is a diagram of the MBG/PGA-PCL composite scaffold prepared in examples 5 and 7 for repairing skull defects of mice in vivo.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, and/or combinations thereof, unless the context clearly indicates otherwise.
In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention will be described in detail below with reference to specific embodiments.
Example 1
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolved; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 15 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 15 drops/min, and continuously stirring for 36 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, continuously evaporating and inducing for 120h at room temperature, then placing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 2h when the temperature is raised to 250 ℃, continuing to raise the temperature to 600 ℃, keeping the temperature at the rate of 1 ℃/min, keeping the temperature for 6h, keeping the whole calcining process in the air atmosphere, closing the box-type furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, and taking out a sample; and finally, grinding the obtained white powder without the template agent, and sieving the ground white powder with a sieve with less than 325 meshes to obtain the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing the bone tissue engineering scaffold material.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 2h at the stirring speed of 3000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.12g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring for 24 hours at the stirring speed of 4000r/min at room temperature, and then performing ultrasonic dispersion for 30 minutes to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 6g of NaCl particles, adding the NaCl particles into the suspension, and continuously stirring for 10min at the stirring speed of 1000 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping is accompanied with stirring at the same time, and the stirring speed is 500 r/min; after the suspension is dripped, continuously stirring the mixture for 30min at a stirring speed of 1000r/min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring the mixture for 3h at the same stirring speed, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing the sediment for 5min at the pressure of 10MPa at room temperature to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 20h under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 96h at the stirring speed of 5000r/min at the interval of 6h, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering.
FIG. 1 is a macroscopic topography map of the prepared mesoporous bioactive glass and a mesoporous structure performance characterization map thereof.
Example 2
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolved; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 15 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 15 drops/min, and continuously stirring for 36 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, and continuously steaming at room temperatureInducing for 120h, then placing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 2h when the temperature rises to 250 ℃, continuing to rise to 600 ℃, keeping the temperature at the rate of 1 ℃/min, keeping the temperature for 6h, keeping the whole calcining process in the air atmosphere, closing the box-type furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, and taking out a sample; and finally, grinding the obtained white powder without the template agent, and sieving the ground white powder with a sieve with less than 325 meshes to obtain the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing the bone tissue engineering scaffold material.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 2h at the stirring speed of 3000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.34g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring for 24 hours at the stirring speed of 4000r/min at room temperature, and then performing ultrasonic dispersion for 30 minutes to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 6g of NaCl particles, adding the NaCl particles into the suspension, and continuously stirring for 10min at the stirring speed of 1000 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping is accompanied with stirring at the same time, and the stirring speed is 500 r/min; after the suspension is dripped, continuously stirring the mixture for 30min at a stirring speed of 1000r/min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring the mixture for 3h at the same stirring speed, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing the sediment for 5min at the pressure of 10MPa at room temperature to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 20h under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 96h at the stirring speed of 5000r/min at the interval of 6h, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering. FIG. 2 is a diagram of a mold used for preparing the MBG/PGA-PCL composite scaffold material and a macroscopic topography of the scaffold material;
example 3
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolved; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 15 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 15 drops/min, and continuously stirring for 36 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, continuously evaporating and inducing for 120h at room temperature, then placing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 2h when the temperature is raised to 250 ℃, continuing to raise the temperature to 600 ℃, keeping the temperature at the rate of 1 ℃/min, keeping the temperature for 6h, keeping the whole calcining process in the air atmosphere, closing the box-type furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, and taking out a sample; and finally, grinding the obtained white powder without the template agent, and sieving the ground white powder with a sieve with less than 325 meshes to obtain the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing the bone tissue engineering scaffold material.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 2h at the stirring speed of 3000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.12g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring for 24 hours at the stirring speed of 4000r/min at room temperature, and then performing ultrasonic dispersion for 30 minutes to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 5g/6g/7g/8g/9g NaCl particles, adding the particles into the suspension, and continuously stirring for 10min at the stirring speed of 1000 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping is accompanied with stirring at the same time, and the stirring speed is 500 r/min; after the suspension is dripped, continuously stirring the mixture for 30min at a stirring speed of 1000r/min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring the mixture for 3h at the same stirring speed, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing the sediment for 5min at the pressure of 10MPa at room temperature to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 20h under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 96h at the stirring speed of 5000r/min at the interval of 6h, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering. FIG. 3 is a graph showing the porosity change of the prepared MBG/PGA-PCL composite scaffold material;
example 4
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolved; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, and continuously stirring for 48 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, continuously evaporating and inducing for 120h at room temperature, then placing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 3h when the temperature is raised to 250 ℃, continuing to raise the temperature to 650 ℃, keeping the temperature at the rate of 1 ℃/min, keeping the temperature for 8h, keeping the whole calcining process in the air atmosphere, closing the box-type furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, and taking out a sample; finally, the obtained white powder without the template agent is ground and sieved by a screen with less than 325 meshes, namelyThe silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing bone tissue engineering scaffold materials is obtained.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 3 hours at the stirring speed of 3000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.12g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring for 20 hours at the stirring speed of 4000r/min at room temperature, and then performing ultrasonic dispersion for 40 minutes to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 6g of NaCl particles, adding the NaCl particles into the suspension, and continuously stirring for 12min at the stirring speed of 1200 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping is accompanied with stirring at the same time, and the stirring speed is 700 r/min; after the suspension is dripped, continuously stirring at the stirring speed of 1200r/min for 50min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring at the same stirring speed for 4h, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing at the room temperature under the pressure of 10MPa for 5min to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 24 hours under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 120 hours at the stirring speed of 5000r/min at the interval of 8 hours, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering. FIG. 4 is a MicroCT three-dimensional reconstruction image and a scanning electron microscope image of the prepared MBG/PGA-PCL composite stent material.
Example 5
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolvedSolving; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, and continuously stirring for 48 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, continuously evaporating and inducing for 120h at room temperature, then placing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 3h when the temperature is raised to 250 ℃, continuing to raise the temperature to 650 ℃, keeping the temperature at the rate of 1 ℃/min, keeping the temperature for 8h, keeping the whole calcining process in the air atmosphere, closing the box-type furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, and taking out a sample; and finally, grinding the obtained white powder without the template agent, and sieving the ground white powder with a sieve with less than 325 meshes to obtain the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing the bone tissue engineering scaffold material.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 3 hours at the stirring speed of 3000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.34g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring for 20 hours at the stirring speed of 4000r/min at room temperature, and then performing ultrasonic dispersion for 40 minutes to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 6g of NaCl particles, adding the NaCl particles into the suspension, and continuously stirring for 12min at the stirring speed of 1200 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping is accompanied with stirring at the same time, and the stirring speed is 700 r/min; after the suspension is dripped, continuously stirring at the stirring speed of 1200r/min for 50min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring at the same stirring speed for 4h, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing at the room temperature under the pressure of 10MPa for 5min to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 24 hours under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 120 hours at the stirring speed of 5000r/min at the interval of 8 hours, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering. FIG. 5 is a distribution diagram of Si, Ca and P elements on the prepared MBG/PGA-PCL composite scaffold material and an EDS energy spectrum.
Example 6
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolved; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, and continuously stirring for 48 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, continuously evaporating and inducing for 120h at room temperature, then placing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 3h when the temperature is raised to 250 ℃, continuing to raise the temperature to 650 ℃, keeping the temperature at the rate of 1 ℃/min, keeping the temperature for 8h, keeping the whole calcining process in the air atmosphere, closing the box-type furnace after the calcining is finished, naturally cooling to room temperature at the room temperature, and taking out a sample; and finally, grinding the obtained white powder without the template agent, and sieving the ground white powder with a sieve with less than 325 meshes to obtain the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing the bone tissue engineering scaffold material.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 3 hours at the stirring speed of 3000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.67g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring for 20 hours at the stirring speed of 4000r/min at room temperature, and then performing ultrasonic dispersion for 40 minutes to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 6g of NaCl particles, adding the NaCl particles into the suspension, and continuously stirring for 12min at the stirring speed of 1200 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping is accompanied with stirring at the same time, and the stirring speed is 700 r/min; after the suspension is dripped, continuously stirring at the stirring speed of 1200r/min for 50min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring at the same stirring speed for 4h, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing at the room temperature under the pressure of 10MPa for 5min to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 24 hours under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 120 hours at the stirring speed of 5000r/min at the interval of 8 hours, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering.
Example 7
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
preparation of mesoporous bioactive glass powder: weighing 60g of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, weighing 1ml of 0.5mol/L hydrochloric acid, adding the absolute ethyl alcohol, weighing 4.0g P123, adding the absolute ethyl alcohol, and stirring at normal temperature until P123 is completely dissolved; then 1.4g Ca (NO) was weighed out3)2·4H2Slowly adding O into the solution while stirring until the O is completely dissolved; measuring 0.69ml TEP, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, measuring 7.3ml TEOS after the TEP is added, slowly dropping the TEP into the solution at a dropping speed of 20 drops/min, and continuously stirring for 48 hours at room temperature after the TEP is completely added to obtain a sol solution; transferring the obtained sol solution to a culture dish, continuously evaporating and inducing at room temperature for 120h, and then performing evaporationPlacing the obtained xerogel in a high-temperature calcining box-type furnace, keeping the temperature for 3h when the temperature rises to 250 ℃, continuing to rise to 700 ℃, keeping the temperature for 7h when the temperature rises to 1 ℃/min, and keeping the temperature for 7h, wherein the box-type furnace is closed after the calcination is finished, and naturally cooling to room temperature at the room temperature, and taking out a sample; and finally, grinding the obtained white powder without the template agent, and sieving the ground white powder with a sieve with less than 325 meshes to obtain the silicon-calcium-phosphorus mesoporous bioactive glass powder which can be used for preparing the bone tissue engineering scaffold material.
Preparing the MBG/PGA-PCL composite scaffold material: measuring 10ml of hexafluoroisopropanol into a small glass bottle, weighing 1g of PGA-PCL to be dissolved in the hexafluoroisopropanol, and continuously stirring for 3h at the stirring speed of 2000r/min at room temperature until the PGA-PCL is completely dissolved; weighing 0.67g of MBG powder, slowly adding the MBG powder into the PGA-PCL solution while stirring, continuously stirring at the stirring speed of 3000r/min for 20h at room temperature, and then performing ultrasonic dispersion for 40min to uniformly disperse the MBG powder into the solution to obtain uniformly dispersed suspension; then weighing 6g of NaCl particles, adding the NaCl particles into the suspension, and continuously stirring for 15min at the stirring speed of 1200 r/min; firstly, measuring 250ml of absolute ethyl alcohol, adding the absolute ethyl alcohol into a beaker, slowly dripping the suspension containing the NaCl particles into an absolute ethyl alcohol solvent through a suction pipe in a fume hood, wherein the dripping speed is 30 drops/min, and the dripping speed is simultaneously accompanied with stirring at the stirring speed of 800 r/min; after the suspension is dripped, continuously stirring at the stirring speed of 1200r/min for 50min, pouring off the absolute ethyl alcohol, then adding 250ml of new absolute ethyl alcohol, continuously stirring at the same stirring speed for 4h, collecting the sediment at the bottom of the beaker, putting the sediment into a stainless steel cylindrical die with the diameter of 12mm, and pressing at the room temperature under the pressure of 10MPa for 5min to obtain a cylindrical sample with the height of 30 mm; and drying the obtained sample for 24 hours under the ventilation condition at room temperature, then putting the sample into a self-made hollow plastic cup, putting the sample into deionized water, continuously stirring and soaking for 120 hours at the stirring speed of 5000r/min at the interval of 8 hours, changing the deionized water once, and finally placing the leached scaffold material under the ventilation condition at room temperature for airing to obtain the final scaffold material for bone tissue engineering.
Example 8
A preparation method of a bone tissue engineering scaffold material comprises the following steps:
(1) preparing mesoporous bioactive glass powder: preparing 1.60mol/L hydrochloric acid in a volumetric flask by using 37% mass concentrated hydrochloric acid, then measuring 50ml of 1.60mol/L hydrochloric acid in a 100ml beaker by using a measuring cylinder, then measuring 1.0g P123(MW 5800) by using an analytical balance to dissolve in 50ml of 1.67mol/L hydrochloric acid, continuously stirring for 1H under the condition of 37 ℃ of a water bath until P123 is completely dissolved, wherein the solution is in a transparent state, then measuring 0.068g of Na3VO 4.12H 2O by using the analytical balance, slowly adding the solution into the solution under the condition of continuous stirring, measuring 1.98g of Ca (NO3) 2.4H 2O after the solution is completely dissolved, slowly adding the solution into the solution under the condition of continuous stirring, dropwise adding 0.309ml of TEP after the solution is completely dissolved, wherein the dropwise adding rate is 12 drops/min, continuously stirring for 15min after the addition is finished, finally dropwise adding 3.049ml of TEOS, the dropwise adding rate is 10 drops/min, and continuously stirring the water bath solution under the condition of 12H after the complete addition, then transferring the mixed solution into a polytetrafluoroethylene reaction kettle liner under the drainage of a glass rod, carrying out hydrothermal reaction for 48 hours at the constant temperature of 100 ℃, after the reaction is finished, naturally cooling the temperature to room temperature, transferring the obtained mixed system liquid into a 50ml centrifuge tube, centrifuging for 5min at 4000r/min, pouring out the supernatant, sequentially washing with distilled water and absolute ethyl alcohol for 3 times respectively, then transferring the powder into a square porcelain boat, placing the square porcelain boat in an electric heating blast drying oven, drying for 12h at the constant temperature of 60 ℃, finally placing the obtained powder in a high-temperature box type calcining furnace, heating to 250 deg.C at a heating rate of 1 deg.C/min in air atmosphere, maintaining for 2h, heating to 600 deg.C at a heating rate of 1 deg.C/min, and then preserving heat for 6h, closing the box furnace after calcination, naturally cooling to room temperature, and taking out a sample to obtain the white mesoporous bioactive glass powder without the template agent.
The other steps were the same as those described in example 7.
The in vivo degradation of the MBG/PGA-PCL composite scaffold materials prepared in examples 4-6 is shown in FIG. 6; the ability of the MBG/PGA-PCL composite scaffold prepared in examples 5 and 7 to repair skull defects of mice in vivo is shown in FIG. 7.
The above description is only a partial example of the present invention combining the actual synthesis technology and principles, the present invention is not limited to the above example, as long as the MBG/PGA-PCL composite bone tissue engineering scaffold material can be prepared within the parameter range related to the technical solution of the present invention, the technical parameter range related to the present invention includes the material ratio for preparing MBG powder; stirring speed and time; the liquid dropping rate; calcining temperature, time and solid-to-liquid ratio of PGA-PCL and hexafluoroisopropanol for preparing the composite scaffold material; MBG doping proportion; the ratio of PGA-PCL to pore-foaming agent; stirring speed and time; the liquid dropping rate; die pressing pressure and time; leaching time, etc.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A bone tissue engineering scaffold material is characterized in that polyglycolide-polycaprolactone two blocks are used as a polymer main body material, and mesoporous bioactive glass powder is used as an inorganic bioactive component; the mesoporous bioactive glass powder is uniformly dispersed in the stent material.
2. The scaffold material for bone tissue engineering according to claim 1, wherein the scaffold material for bone tissue engineering has a pore size of 300-450 μm and a porosity of 75-80%.
3. The bone tissue engineering scaffold material according to claim 1, wherein the mesoporous bioactive glass powder is 10-40% by weight of the bone tissue engineering scaffold material.
4. The bone tissue engineering scaffold material according to claim 1 or 3, wherein said mesoporous bioactive glass powder is a silicon-calcium-phosphorus mesoporous bioactive glass powder or a vanadium-doped silicon-calcium-phosphorus mesoporous bioactive glass powder.
5. The scaffold material for bone tissue engineering according to claim 4, wherein SiO is contained in the mesoporous bioactive glass powder containing Si, Ca and P2、CaO、P2O5In a molar ratio of 50-100:15: 2.5; the macro morphology is a cluster or chain structure formed by bonding and twisting vermicular structures, the grain diameter of the vermicular particles is 0.5-2 mu m, and the specific surface area is more than 300m2The mesoporous aperture is larger than 6nm and the macroscopic particle size is smaller than 45 mu m.
6. The method for preparing the bone tissue engineering scaffold material of any one of claims 1 to 5, comprising the steps of:
dissolving polyglycolide-polycaprolactone in hexafluoroisopropanol, adding mesoporous bioactive glass powder, uniformly dispersing in the solution, and adding a pore-forming agent; granulating, molding, and filtering.
7. The process according to claim 6, wherein the solid-to-liquid ratio of polyglycolide-polycaprolactone to hexafluoroisopropanol is 10 to 15% w/v; the mass ratio of the polyglycolide-polycaprolactone to the pore-foaming agent is 1: 6-7.
8. The method according to claim 6, wherein the method for uniformly dispersing the mesoporous bioactive glass powder in the solution comprises: after the mesoporous bioactive glass powder is added into the solution, continuously stirring for 12-24h at 4000r/min of 2000-.
9. The method as claimed in claim 6 or 7, wherein the pore-forming agent is sodium chloride, and the particle size of the sodium chloride is 300-450 μm.
10. The method for preparing according to claim 6, characterized in that said granulation comprises the following steps: dropwise adding the solution added with the pore-foaming agent into absolute ethyl alcohol for granulation, wherein the dropwise adding speed is 20-30 drops/min, stirring is carried out simultaneously, and the stirring speed is 500-;
preferably, the press mold comprises the steps of: after granulation, continuously stirring for 30-50min at the stirring speed of 1000-2000r/min, pouring off the absolute ethyl alcohol, adding new absolute ethyl alcohol, continuously stirring for 1-5h at the same stirring speed, collecting precipitate, putting the precipitate into a mold, and pressing for 3-5min at the pressure of 8-12MPa to obtain a sample;
preferably, the leaching comprises the steps of: and drying the pressed sample for 12-24h under the ventilation condition at room temperature, then putting the sample into water at 4000-6000r/min, stirring and soaking for 72-120h, changing water once at intervals of 6-12h, observing the leaching condition of the scaffold at any time to adjust the stirring speed, and finally airing the leached scaffold material under the ventilation condition at room temperature to obtain the final scaffold material for bone tissue engineering.
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