CN113112899B - Simulation research device for premature rupture of fetal membranes - Google Patents
Simulation research device for premature rupture of fetal membranes Download PDFInfo
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- CN113112899B CN113112899B CN202110438269.8A CN202110438269A CN113112899B CN 113112899 B CN113112899 B CN 113112899B CN 202110438269 A CN202110438269 A CN 202110438269A CN 113112899 B CN113112899 B CN 113112899B
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- 238000004088 simulation Methods 0.000 title claims abstract description 24
- 238000011160 research Methods 0.000 title claims abstract description 23
- 208000003107 Premature Rupture Fetal Membranes Diseases 0.000 title claims abstract description 7
- 206010036603 Premature rupture of membranes Diseases 0.000 claims description 20
- 239000007787 solid Substances 0.000 claims description 6
- 210000004379 membrane Anatomy 0.000 claims description 4
- 239000012188 paraffin wax Substances 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 2
- 210000002219 extraembryonic membrane Anatomy 0.000 abstract description 45
- 230000002028 premature Effects 0.000 abstract description 29
- 230000000694 effects Effects 0.000 abstract description 4
- 238000010171 animal model Methods 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000007792 addition Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 1
- 208000032571 Infant acute respiratory distress syndrome Diseases 0.000 description 1
- 206010056254 Intrauterine infection Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010051606 Necrotising colitis Diseases 0.000 description 1
- 206010028974 Neonatal respiratory distress syndrome Diseases 0.000 description 1
- 206010036595 Premature delivery Diseases 0.000 description 1
- 208000007313 Reproductive Tract Infections Diseases 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 208000004995 necrotizing enterocolitis Diseases 0.000 description 1
- 201000002652 newborn respiratory distress syndrome Diseases 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 201000006195 perinatal necrotizing enterocolitis Diseases 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
- G09B23/281—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine for pregnancy, birth or obstetrics
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- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Algebra (AREA)
- Mathematical Analysis (AREA)
- General Health & Medical Sciences (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Reproductive Health (AREA)
- Pregnancy & Childbirth (AREA)
- Computational Mathematics (AREA)
- Gynecology & Obstetrics (AREA)
- Chemical & Material Sciences (AREA)
- Mathematical Optimization (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
- Business, Economics & Management (AREA)
- Educational Administration (AREA)
- Educational Technology (AREA)
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Abstract
The invention relates to a simulation research device for premature rupture of fetal membranes, which comprises a culture box body and a culture top cover; the culture box body is internally provided with a groove, a protruding block is arranged in the middle of the groove, the protruding block is connected with a detachable cylinder in a threaded manner, and a connector is arranged in the culture box body; the connecting body is internally provided with a top end device, an elastic rubber valve with holes is further arranged in the middle position above the top end device, and the elastic rubber valve with holes is communicated with the inside of the connecting body. The advantages are as follows: the invention simulates the occurrence of premature rupture of the fetal membranes by simulating the intrauterine pressure and carrying out corresponding experimental intervention by combining with the real fetal membrane tissue of a human body, and carries out corresponding molecular biological means to detect the fetal membrane tissue after the occurrence, so as to research the mechanism of occurrence of premature rupture of the fetal membranes, provide a convenient experimental research device for research on premature rupture of the fetal membranes of obstetrical department, and fill the gap that the current research on dynamic activity of premature rupture of the fetal membranes at home and abroad can only use animal models.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a simulation research device for premature rupture of a fetal membrane.
Background
Premature rupture of the membranes (premature rupture of membranes, PROM) refers to the natural rupture of the membranes just before birth. The term premature rupture of membranes is called by those who reach gestation over 37 weeks; those who did not reach 37 weeks were called premature rupture of the premature membranes. The occurrence of premature rupture of the fetal membranes is mainly associated with genital tract infection, increased amniotic cavity pressure, uneven stress of the fetal membranes, lack of nutrition and the like; however, premature rupture of the fetal membranes is a common complication of pregnant women, premature rupture of the fetal membranes at term is extremely easy to cause infection and premature delivery of the fetus, premature infants, premature pregnancy Zhou Yue is early, and the greater the risk of chorionic amniotis, various complications caused by premature infant failure and intrauterine infection, including neonatal respiratory distress syndrome, cerebral hemorrhage, necrotizing enterocolitis and the like, cause high morbidity and high mortality of the neonates, bring heavy burden to the quality of the birth population and the socioeconomic performance, so that rupture of the fetal membranes can be found early, and the pregnant women and the fetus can be correspondingly diagnosed and treated, thereby having important significance for controlling related complications. In view of such serious consequences, scientific researchers in the relevant arts have continuously sought a mechanism of occurrence of premature rupture of membranes, thereby preventing and preventing occurrence of premature rupture of membranes as early as possible. However, the related research on premature rupture of fetal membranes is very slow, wherein one important reason is that premature rupture of fetal membranes occurs in pregnant women, a human body model cannot be established due to legal ethical restrictions, a corresponding animal research model is difficult to establish, and research results are easy to deviate due to human-animal differences, so that clinical prevention cannot be guided accurately.
Searching for the present patent does not find a similar technology.
Therefore, in view of the above-mentioned problems, the present invention provides a physical device capable of simulating intrauterine pressure and performing corresponding experimental intervention in combination with human real fetal membrane tissue, and the device can simulate intrauterine environment in combination with corresponding chemical liquid; meanwhile, after the device is designed, the intervention can be carried out by adopting corresponding intervention factors such as inflammatory factors, small molecular compounds and the like, whether premature rupture of the fetal membranes occurs or not can be known by comparison and the like, and detection can be carried out by corresponding molecular biological means after the premature rupture of the fetal membranes occurs, so that the device is beneficial to carrying out intensive study on the occurrence mechanism of the premature rupture of the fetal membranes; the device is particularly a simulation research device for premature rupture of the fetal membranes, but the novel simulation research device for premature rupture of the fetal membranes is not reported at present.
Disclosure of Invention
The invention aims to solve the problems and provides a simulation research device for premature rupture of a fetal membrane.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a simulation research device for premature rupture of fetal membranes comprises a cylindrical culture box body and a culture top cover positioned on the culture body, wherein the culture box body is in threaded connection with the culture top cover; the culture box body is internally provided with a groove A, the groove A is positioned at the upper half part of the culture box body, the lower half part of the culture box body is also provided with a groove B, the middle position of the groove B is provided with a threaded protruding block, the protruding block is connected with a detachable cylinder in a threaded manner, the top end surface of the detachable cylinder is a paraffin surface, the interior of the culture box body is provided with a connector which is in a convex structure and consists of an upper hollow cylinder and a lower hollow cylinder with different diameters, the upper half part of the connector is embedded in the groove A in the culture box body, the lower half part of the connector is embedded in the groove B in the culture box body, and the cylinder in the groove B is positioned in the connector; the hollow cylinder of the upper half part of the connector is internally provided with a top end device, the top end device is of a cylinder structure, a groove C is further formed in the middle position of the bottom of the top end device, a concave structure is formed between the groove C and the top end device, one end of the top end device with the groove C is arranged downwards, an elastic porous rubber valve is further arranged in the middle position above the top end device, and the elastic porous rubber valve is communicated with the groove C.
In the above-mentioned simulation study device for premature rupture of membranes, as a preferred embodiment, the diameter of the groove a is larger than that of the groove B, and the excess diameter length is a solid portion of the culture cassette body, and the upper half portion of the connector is seated on the solid portion of the culture cassette body.
In the above-mentioned simulation study device for premature rupture of membranes, as a preferable mode, the height of the cylinder is kept identical to the height of the hollow cylinder in the lower half of the connector.
In the above-mentioned simulation research device for premature rupture of membranes, as an preferable scheme, the two sides of the connector are provided with small grooves embedded in the two sides of the connector and downward integrally, the small grooves sequentially penetrate through the upper half and the lower side of the connector from top to bottom, the bottom side of the lower half of the connector is further provided with a slot hole penetrating through the inner bottom edge of the lower half of the connector transversely, and the slot hole is communicated with the small grooves on the two sides of the connector.
In the above-mentioned simulation research device for premature rupture of membranes, as a preferable mode, elastic clips are arranged on two sides of the top end of the upper half part of the connector, and the clips are fixedly connected with the upper half part of the connector.
The invention has the advantages that:
1. the invention has simple operation and strong practicability, and can be used for carrying out corresponding experimental intervention by simulating the intrauterine pressure and combining with the real fetal membrane tissue of a human body, thereby realizing the technologies of comparing, analyzing and knowing whether the premature rupture of the fetal membrane occurs, carrying out corresponding molecular biological means for detection after the premature rupture, and the like, being beneficial to carrying out various experimental researches while keeping the activity of the fetal membrane, providing a convenient and effective experimental device for the related research of the fetal membrane of obstetrical department, and being suitable for large-scale popularization and application.
Drawings
FIG. 1 is a schematic diagram of a simulation study device for premature rupture of membranes according to the present invention.
FIG. 2 is a schematic view of a part of a simulation study device for premature rupture of membranes according to the present invention.
FIG. 3 is a schematic view of a partial cross-sectional cut-away of a simulation study device for premature rupture of membranes according to the present invention.
FIG. 4 is a schematic diagram of the structure of the connector on the simulation study device for premature rupture of membranes according to the present invention.
Detailed Description
The invention is further described below in conjunction with the detailed description. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. Further, it is understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the description of the present invention, and such equivalents are intended to fall within the scope of the claims appended hereto.
Reference numerals and components referred to in the drawings are as follows:
1. culture box body 2, culture top cover 3 and groove A
4. Groove B5, bump 6, cylinder
7. Connector 8 tip device 9 groove C
10. Elastic rubber valve 11 with holes, small groove 12, slotted hole
13. Elastic clamp
Example 1A simulation study device for premature rupture of membranes
Referring to fig. 1-4, fig. 1 is a schematic structural diagram of a simulation study device for premature rupture of membranes according to the present invention. FIG. 2 is a schematic view of a part of a simulation study device for premature rupture of membranes according to the present invention. FIG. 3 is a schematic view of a partial cross-sectional cut-away of a simulation study device for premature rupture of membranes according to the present invention. FIG. 4 is a schematic diagram of the structure of the connector on the simulation study device for premature rupture of membranes according to the present invention.
The invention mainly designs a physical device which can simulate intrauterine pressure and can be combined with human real fetal membrane tissues to perform corresponding experimental intervention, the device can be combined with corresponding chemical liquid to simulate intrauterine environment, intervention can be performed by adopting corresponding intervention factors such as inflammatory factors, small molecular compounds and the like after the design is finished, comparison and the like are performed to know whether premature rupture of the fetal membrane occurs or not, and detection and the like are performed by corresponding molecular biological means after the occurrence so as to improve clinical experimental results, and the device particularly relates to a simulation research device for premature rupture of the fetal membrane, which mainly comprises a cylindrical culture box body 1 and a culture top cover 2 positioned on the culture body, wherein the culture box body 1 is in threaded connection with the culture top cover 2; the culture box body 1 inside be provided with a recess A3, recess A3 be located culture box body 1's first half, and still be provided with a recess B4 at culture box body 1's latter half, recess B4's intermediate position be provided with a threaded boss piece 5, boss piece 5 on threaded connection can dismantle cylinder 6, just can dismantle cylinder 6 under the state that needs to increase the culture solution for the paraffin face, reduce the occupation space in the culture box body, make things convenient for the abundant contact between different culture solutions and the fetal membrane, and then realize carrying out the contrast of different degree of variation to the fetal membrane tissue, improve the accuracy of data experiment, and can avoid cell adherence to follow-up dismantlement influence such as cell loss can not cause for the top face of dismantling the cylinder for the setting of paraffin face; the inside of the culture box body 1 is provided with a connector 7, the connector 7 is in a convex structure and consists of an upper hollow cylinder and a lower hollow cylinder with different diameters, the upper half part of the connector 7 is embedded in a groove A3 in the culture box body, the lower half part of the connector 7 is embedded in a groove B4 of the culture box body, the cylinders in the groove B4 are positioned in the connector 7, the connector 7 is used for separating and communicating intervention liquid in an upper device and a lower device, the inner surface of the connector is a main operation surface, and the connector can be used for placing and culturing intima tissues; a top end device 8 is further arranged in the hollow cylinder at the upper half part of the connector 7, the top end device 8 is of a cylinder structure, a groove C9 is further formed in the middle position of the bottom of the top end device 8, a concave structure is formed between the groove C9 and the top end device 8, and one end of the top end device with the groove C is arranged downwards; and an elastic porous rubber valve 10 is further arranged in the middle position above the top end device 8, the elastic porous rubber valve 10 is communicated with the groove C9, the top end device 8 can be used for sealing the upper device after a fetal membrane tissue covers a culture surface, and meanwhile, the elastic porous rubber valve 10 can be penetrated through devices such as a syringe to carry out gas pressurization or culture solution addition so as to simulate the intrauterine pressure and carry out experimental intervention, further, the purposes of comparing and knowing whether the premature rupture of the fetal membrane can occur or not and carrying out corresponding molecular biological means for detection after the premature rupture of the fetal membrane occur are achieved, the clinical experimental result of the premature rupture of the fetal membrane is improved, and the occurrence of complications is reduced.
In this embodiment, it is preferable that the diameter of the groove A3 is larger than that of the groove B4, and the excess diameter length is a solid portion of the culture cassette, and the upper half of the connector 7 is seated on the solid portion of the culture cassette 1.
In this embodiment, the height of the cylinder 6 is preferably consistent with the height of the hollow cylinder in the lower half of the connector 7, and the arrangement can keep the whole plane equal, which is beneficial to the whole and comprehensive contact of the fetal membrane and the plane and the tissue culture.
In this embodiment, preferably, the two sides of the connector 7 are provided with small grooves 11 embedded in the two sides of the connector and wholly downward, the small grooves 11 sequentially penetrate through the upper half part and the lower side part of the connector 7 from top to bottom, the bottom side of the lower half part of the connector 7 is also provided with a slot 12 which transversely penetrates through the inner bottom edge of the lower half part of the connector, the slot 12 is communicated with the small grooves 11 on the two sides of the connector, and the small grooves 11 on the two sides of the connector and the slot 12 are arranged, so that the tight fit between the connector and the culture box can be avoided, and the culture solution cannot enter the culture box, so that an operator can conveniently fill the culture box with liquid, and convenience is provided for the later-stage simulated culture work.
In this embodiment, preferably, the top both sides of the upper half of the connector 7 are provided with elastic clips 13, and the clips 13 are fixedly connected with the upper half of the connector 7, the elastic clips 13 are made of rubber materials and have certain toughness and elasticity, so that the top device is convenient to enter and take out, and meanwhile, the top device is prevented from slipping off, which is beneficial to forming a closed air pressure environment, and is convenient for a doctor to diagnose and detect the top device.
The application method of the invention comprises the following steps: firstly, placing a connector in a groove B of a culture box body (the height of a cylinder in the groove B is kept horizontal with the height of a hollow cylinder in the lower half part of the connector), then adding culture solution into the culture box body through the connector, wherein the added culture solution is about 0.5cm higher than a culture surface, then placing a taken fetal membrane tissue on the culture surface, immersing the fetal membrane tissue by the culture solution, and then placing the fetal membrane tissue in a 37-DEG incubator for culture, so that the fetal membrane tissue surface is fully attached to the culture surface; when the fetal membrane tissue is fully attached to the culture dish, the top end device is placed in the connector, the fetal membrane tissue is physically paved on the contact surface by hand, then the culture is carried out, and after the fetal membrane tissue cells are attached to the wall and grow, the sealing effect is achieved,) the fetal membrane tissue and the culture surface are fully closed, meanwhile, the pressure in the uterine cavity can be simulated by pressurizing an injector through an elastic porous rubber valve arranged on the top end device, and the authenticity of experimental data is improved; and when experimental intervention is carried out, the cylinder in the culture box body is disassembled and taken out, and the culture solution is filled into the culture box body or the culture solution with the intervention factors is added into the culture box body through the connecting external groove, so that experimental intervention operation is carried out.
It should be noted that: the invention has simple operation and strong practicability, and can be used for carrying out corresponding experimental intervention by simulating the intrauterine pressure and combining with the real fetal membrane tissue of a human body, thereby realizing the technologies of comparing, analyzing and knowing whether the premature rupture of the fetal membrane occurs, carrying out corresponding molecular biological means for detection after the premature rupture, and the like, being beneficial to carrying out various experimental researches while keeping the activity of the fetal membrane, providing a convenient and effective experimental device for the related research of the fetal membrane of obstetrical department, and being suitable for large-scale popularization and application.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and additions to the present invention may be made by those skilled in the art without departing from the principles of the present invention and such modifications and additions are to be considered as well as within the scope of the present invention.
Claims (4)
1. The simulation research device for premature rupture of fetal membranes is characterized by comprising a cylindrical culture box body and a culture top cover positioned on the culture body, wherein the culture box body is in threaded connection with the culture top cover; the culture box body is internally provided with a groove A, the groove A is positioned at the upper half part of the culture box body, the lower half part of the culture box body is also provided with a groove B, the middle position of the groove B is provided with a threaded protruding block, the protruding block is connected with a detachable cylinder in a threaded manner, the top end surface of the detachable cylinder is a paraffin surface, the interior of the culture box body is provided with a connector which is in a convex structure and consists of an upper hollow cylinder and a lower hollow cylinder with different diameters, the upper half part of the connector is embedded in the groove A in the culture box body, the lower half part of the connector is embedded in the groove B in the culture box body, and the cylinder in the groove B is positioned in the connector; the upper half part of the connecting body is of a cylindrical structure, a groove C is formed in the middle position of the bottom of the top device, a concave structure is formed between the groove C and the top device, one end of the top device with the groove C is arranged downwards, an elastic porous rubber valve is further arranged in the middle position above the top device, the elastic porous rubber valve is communicated with the groove C, the diameter of the groove A is larger than that of the groove B, the excessive diameter length is the solid part of the culture box body, the upper half part of the connecting body is located on the solid part of the culture box body, the inner face of the connecting body is a main operation face, the connecting body can be used for placing and culturing inner membrane tissues, the elastic porous rubber valve arranged on the top device is used for pressurizing an injector, and the pressure in a uterine cavity is simulated.
2. A simulation study apparatus for premature rupture of membranes according to claim 1, wherein the height of said cylinder is maintained in conformity with the height of the hollow cylinder in the lower part of the connector.
3. The device for simulating premature rupture of membranes according to claim 1, wherein small grooves which are embedded in the two side edges of the connector and integrally downward are formed in the two side edges of the connector, the small grooves penetrate through the upper half part and the lower side part of the connector from top to bottom in sequence, slotted holes which penetrate through the inner bottom edges of the lower half part of the connector transversely are formed in the side edges of the bottom half part of the connector, and the slotted holes are communicated with the small grooves in the two side edges of the connector.
4. A device for simulating premature rupture of membranes according to claim 1, wherein the upper part of the connector is provided with resilient clips on opposite sides of its top end, and wherein said clips are fixedly connected to the upper part of the connector.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110438269.8A CN113112899B (en) | 2021-04-22 | 2021-04-22 | Simulation research device for premature rupture of fetal membranes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110438269.8A CN113112899B (en) | 2021-04-22 | 2021-04-22 | Simulation research device for premature rupture of fetal membranes |
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| CN113112899A CN113112899A (en) | 2021-07-13 |
| CN113112899B true CN113112899B (en) | 2024-03-22 |
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| JP2016202076A (en) * | 2015-04-22 | 2016-12-08 | 学校法人近畿大学 | Culture apparatus, and cell culture method using culture apparatus |
| CN107603878A (en) * | 2017-09-05 | 2018-01-19 | 广东省第二人民医院 | Cell culture apparatus and its method under a kind of in-vitro simulated pressing environment |
| CN110491234A (en) * | 2019-08-29 | 2019-11-22 | 北京航空航天大学 | A kind of experimental provision of mechanics of biomembranes pressure and mechanical property |
-
2021
- 2021-04-22 CN CN202110438269.8A patent/CN113112899B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006212017A (en) * | 2005-02-03 | 2006-08-17 | Otake:Kk | Apparatus for carrying out rotational culture under loading and pressure |
| CN101421388A (en) * | 2006-01-16 | 2009-04-29 | 利莱恩斯生命科学有限公司 | Device for culturing and transporting cells |
| CN202003576U (en) * | 2011-03-21 | 2011-10-05 | 中国人民解放军第四军医大学 | In-vitro placental barrier model |
| CN103740591A (en) * | 2014-01-17 | 2014-04-23 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture device and application thereof |
| JP2016202076A (en) * | 2015-04-22 | 2016-12-08 | 学校法人近畿大学 | Culture apparatus, and cell culture method using culture apparatus |
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