CN113112899A - Simulation research device for premature rupture of fetal membranes - Google Patents
Simulation research device for premature rupture of fetal membranes Download PDFInfo
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- CN113112899A CN113112899A CN202110438269.8A CN202110438269A CN113112899A CN 113112899 A CN113112899 A CN 113112899A CN 202110438269 A CN202110438269 A CN 202110438269A CN 113112899 A CN113112899 A CN 113112899A
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- 238000011160 research Methods 0.000 title claims abstract description 35
- 238000004088 simulation Methods 0.000 title claims abstract description 27
- 208000003107 Premature Rupture Fetal Membranes Diseases 0.000 title description 2
- 206010036603 Premature rupture of membranes Diseases 0.000 claims abstract description 29
- 239000007787 solid Substances 0.000 claims description 5
- 239000012188 paraffin wax Substances 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 210000002219 extraembryonic membrane Anatomy 0.000 abstract description 45
- 230000002028 premature Effects 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 2
- 238000010171 animal model Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 210000004379 membrane Anatomy 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 206010036590 Premature baby Diseases 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010060937 Amniotic cavity infection Diseases 0.000 description 1
- 208000008158 Chorioamnionitis Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 208000032571 Infant acute respiratory distress syndrome Diseases 0.000 description 1
- 206010056254 Intrauterine infection Diseases 0.000 description 1
- 206010051606 Necrotising colitis Diseases 0.000 description 1
- 206010028974 Neonatal respiratory distress syndrome Diseases 0.000 description 1
- 206010036595 Premature delivery Diseases 0.000 description 1
- 208000007313 Reproductive Tract Infections Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000000185 intracerebroventricular administration Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 208000004995 necrotizing enterocolitis Diseases 0.000 description 1
- 201000002652 newborn respiratory distress syndrome Diseases 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 201000006195 perinatal necrotizing enterocolitis Diseases 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
- G09B23/281—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine for pregnancy, birth or obstetrics
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- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Reproductive Health (AREA)
- Pregnancy & Childbirth (AREA)
- Computational Mathematics (AREA)
- Gynecology & Obstetrics (AREA)
- Chemical & Material Sciences (AREA)
- Mathematical Optimization (AREA)
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Abstract
The invention relates to a simulation research device for premature rupture of membranes, which comprises a culture box body and a culture top cover; a groove is formed in the culture box body, a protruding block is arranged in the middle of the groove, a detachable cylinder is connected to the protruding block in a threaded mode, and a connector is arranged in the culture box body; the connecting body is internally provided with a top end device, an elastic porous rubber valve is further arranged in the middle above the top end device, and the elastic porous rubber valve is communicated with the inside of the connecting body. Its advantages are: the invention simulates the occurrence of premature rupture of the fetal membrane by simulating the pressure in the uterine cavity and carrying out corresponding experimental intervention by combining with the real fetal membrane tissue of a human body, detects the fetal membrane tissue by corresponding molecular biological means after the occurrence of the premature rupture of the fetal membrane, researches the occurrence mechanism of the premature rupture of the fetal membrane, provides a convenient experimental research device for the research of the premature rupture of the fetal membrane in obstetrical department, and fills the gap that the dynamic activity research of the premature rupture of the fetal membrane at home and abroad can only use an animal model at present.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a simulation research device for premature rupture of membranes.
Background
Premature rupture of membranes (PROM) refers to the natural rupture of membranes before birth. The premature rupture of the membrane is called if the pregnancy reaches or exceeds 37 weeks; premature rupture of the membrane is said to occur in less than 37 weeks. The premature rupture of the fetal membranes is mainly caused by genital tract infection, increased pressure of amniotic cavities, uneven stress of the fetal membranes, lack of nutrition and the like; the premature rupture of the fetal membrane is a common complication of women in gestation period, the premature rupture of the fetal membrane in an immature period is easy to cause infection and premature delivery of a fetus, premature babies are born, the earlier the gestation period is, the higher the risk of chorioamnionitis is, various complications caused by premature baby immature and intrauterine infection comprise neonatal respiratory distress syndrome, intracerebroventricular hemorrhage, necrotizing enterocolitis and the like, the high morbidity and mortality of the newborn are caused, heavy burden is brought to the birth population quality and social economy, and therefore the rupture of the fetal membrane is discovered as soon as possible, and corresponding diagnosis, treatment and treatment are carried out on the pregnant woman and the fetus, and the premature rupture of the fetal membrane has important significance for controlling related complications. In view of such serious consequences, scientific researchers of related practitioners have continuously searched for mechanisms of premature rupture of membranes, so as to prevent and prevent premature rupture of membranes as early as possible. However, the related research on premature rupture of membranes is very slow, one important reason is that premature rupture of membranes occurs in pregnant women, and due to legal ethical limitations, a human model cannot be established, and the establishment of a corresponding animal research model is very difficult, and due to differences between people and animals, the research result is prone to deviation, and clinical prevention cannot be accurately guided.
Searching the current patent does not find a similar technology.
Therefore, in view of the above problems, the present invention provides a physical device capable of simulating the pressure in the uterine cavity and performing corresponding experimental intervention by combining with the real fetal membrane tissue of the human body, and the device can simulate the intrauterine environment by combining with corresponding chemical liquid; meanwhile, after the design of the device is finished, intervention can be carried out by adopting corresponding intervention factors such as inflammatory factors and small molecular compounds, comparison and the like can be carried out to know whether premature rupture of the fetal membranes occurs or not, and after the premature rupture of the fetal membranes occurs, corresponding molecular biological means can be carried out for detection and the like, so that intensive research on the premature rupture of the fetal membranes can be facilitated; the device is specifically a simulation research device for premature rupture of membranes, but no report is found about the novel simulation research device for premature rupture of membranes at present.
Disclosure of Invention
The invention aims to solve the problems and particularly provides a simulation research device for premature rupture of a fetal membrane.
In order to achieve the purpose, the invention adopts the technical scheme that:
a simulation research device for premature rupture of membranes comprises a cylindrical culture box body and a culture top cover positioned on the culture body, wherein the culture box body is in threaded connection with the culture top cover; the culture box body is internally provided with a groove A, the groove A is positioned at the upper half part of the culture box body, the lower half part of the culture box body is also provided with a groove B, the middle position of the groove B is provided with a convex block with threads, the convex block is in threaded connection with a detachable cylinder, the top end surface of the detachable cylinder is a paraffin surface, the interior of the culture box body is provided with a connector, the connector is in a convex structure and consists of an upper hollow cylinder and a lower hollow cylinder with different diameters, the upper half part of the connector is embedded in the groove A in the culture box body, the lower half part of the connector is embedded in the groove B of the culture box body, and the cylinder in the groove B is positioned in the connector; still be provided with the top end device in the hollow cylinder of the first half of connector, the top end device be the cylinder structure, its the intermediate position of top end device bottom still is provided with a recess C, recess C and top end device between be concave character type structure, and the top end device has the one end of recess C and sets up down, and the intermediate position of top end device top still is provided with the porose rubber valve of elasticity, just porose rubber valve of elasticity communicate with each other with recess C.
In the simulation research device for premature rupture of membranes, as a preferred embodiment, the diameter of the groove a is greater than that of the groove B, and the length of the excess diameter is the solid part of the culture box body, and the upper half part of the connector is located on the solid part of the culture box body.
In the simulation research device for premature rupture of membranes, the height of the cylinder is preferably consistent with that of the hollow cylinder in the lower half part of the connector.
In the simulation research device for premature rupture of membranes, as a preferred scheme, two side edges of the connector are provided with small grooves which are embedded in the two side edges of the connector and are downward integrally, the small grooves sequentially penetrate through the upper half part and the lower half part of the connector from top to bottom, a slotted hole transversely penetrating through the bottom edge of the lower half part of the connector is further formed in the side edge of the bottom of the lower half part of the connector, and the slotted hole is communicated with the small grooves in the two side edges of the connector.
In the simulation research device for premature rupture of membranes, as a preferred embodiment, elastic clips are arranged on two sides of the top end of the upper half part of the connecting body, and the clips are fixedly connected with the upper half part of the connecting body.
The invention has the advantages that:
1. the invention has simple operation and strong practicability, carries out corresponding experimental intervention by simulating the pressure in the uterine cavity and combining with the real fetal membrane tissue of a human body, thereby comparing, analyzing and knowing whether the premature rupture of the fetal membrane occurs or not, carrying out corresponding molecular biology means for detection after the premature rupture occurs and the like, being beneficial to carrying out various experimental researches while keeping the activity of the fetal membrane, providing a convenient and effective experimental device for the relevant researches of the fetal membrane in obstetrical department, and being suitable for large-scale popularization and application.
Drawings
FIG. 1 is a schematic structural diagram of a simulation research device for premature rupture of membranes in the invention.
Fig. 2 is a partial structural schematic diagram of a simulation research device for premature rupture of membranes in the invention.
Fig. 3 is a schematic view of a partial cross-sectional cut-away structure of a simulation study device for premature rupture of membranes in the invention.
FIG. 4 is a schematic structural diagram of the connecting body of the simulation research device for premature rupture of membranes in the invention.
Detailed Description
The invention will be further illustrated with reference to specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications can be made by those skilled in the art after reading the disclosure of the present invention, and equivalents fall within the scope of the appended claims.
The reference numerals and components referred to in the drawings are as follows:
1. culture box body 2, culture top cover 3, groove A
4. Groove B5, convex block 6, cylinder
7. Connector 8, top end device 9, groove C
10. Elastic porous rubber valve 11, small groove 12, groove hole
13. Elastic clamp
Referring to fig. 1-4, fig. 1 is a schematic structural diagram of a simulation research device for premature rupture of membranes according to the present invention. Fig. 2 is a partial structural schematic diagram of a simulation research device for premature rupture of membranes in the invention. Fig. 3 is a schematic view of a partial cross-sectional cut-away structure of a simulation study device for premature rupture of membranes in the invention. FIG. 4 is a schematic structural diagram of the connecting body of the simulation research device for premature rupture of membranes in the invention.
The invention mainly designs a physical device which can simulate the pressure in a uterine cavity and can be combined with real fetal membrane tissues of a human body to perform corresponding experimental intervention, the device can be combined with corresponding chemical liquid to simulate the intrauterine environment, and can intervene by adopting corresponding intervention factors such as inflammatory factors, small molecular compounds and the like after the design is finished, and can compare and know whether premature rupture of the fetal membrane occurs or not, and perform detection and the like by corresponding molecular biology means after the premature rupture of the fetal membrane occurs so as to improve the clinical experimental result, the device is specifically a simulation research device for the premature rupture of the fetal membrane, the device mainly comprises a cylindrical culture box body 1 and a culture top cover 2 positioned on the culture body, and the culture box body 1 is in threaded connection with the culture top cover 2; the culture box body 1 is internally provided with a groove A3, the groove A3 is positioned at the upper half part of the culture box body 1, the lower half part of the culture box body 1 is also provided with a groove B4, a threaded protruding block 5 is arranged at the middle position of the groove B4, the protruding block 5 is in threaded connection with a detachable cylinder 6, the top end surface of the detachable cylinder is a paraffin surface, the cylinder 6 can be detached in a state that culture solution needs to be added, the occupied space in the culture box body is reduced, the different culture solutions can be in full contact with the fetal membranes conveniently, further, the fetal membrane tissues can be compared in different change degrees, the accuracy of data experiments is improved, the top end surface of the detachable cylinder is a paraffin surface, cells can be prevented from adhering to the wall, and the like cannot be influenced by the subsequent detachment due to cell loss; the culture box body 1 is internally provided with a connector 7, the connector 7 is of a convex structure and consists of an upper hollow cylinder and a lower hollow cylinder with different diameters, the upper half part of the connector 7 is embedded in a groove A3 in the culture box body, the lower half part of the connector is embedded in a groove B4 in the culture box body, the cylinders in the groove B4 are positioned in the connector 7, the connector 7 is used for separating and communicating dry pre-liquid in an upper device and a lower device, and the inner surface of the connector is a main operation surface and can be used for placing and culturing intima tissues; a top end device 8 is further arranged in the hollow cylinder of the upper half part of the connecting body 7, the top end device 8 is of a cylindrical structure, a groove C9 is further arranged in the middle of the bottom of the top end device 8, a concave structure is formed between the groove C9 and the top end device 8, and one end, provided with the groove C, of the top end device faces downwards; and the intermediate position above the top end device 8 is also provided with an elastic porous rubber valve 10, and the elastic porous rubber valve 10 is communicated with the groove C9, the top end device 8 can be used for sealing the device after the fetal membrane tissue covers the culture surface, and meanwhile, the elastic porous rubber valve 10 can be penetrated by devices such as an injector and the like to pressurize gas or add culture solution so as to simulate intrauterine pressure and perform intervention of experiments, and further compare and know whether premature rupture of the fetal membrane occurs, and perform corresponding molecular biology means for detection after occurrence, thereby achieving the purpose of detecting the fetal membrane of a pregnant woman, improving the experimental result of the premature rupture of the clinical fetal membrane, and reducing the occurrence of complications.
In this embodiment, it is preferable that the diameter of the groove A3 is larger than that of the groove B4, and the length of the diameter is larger than that of the solid portion of the culture box body on which the upper half of the connecting body 7 is seated on the solid portion of the culture box body 1.
In this embodiment, the height of the cylinder 6 is preferably consistent with the height of the hollow cylinder in the lower half part of the connector 7, and this arrangement can keep the whole plane equal, which is beneficial to the whole contact between the fetal membrane and the plane and the tissue culture.
In this embodiment, it is preferred both sides limit of connector 7 is provided with embedded in connector both sides limit and whole decurrent little recess 11, little recess 11 from the top down run through the first half and the lower limb of connector 7 in proper order, just the bottom side of connector 7 the latter half still is provided with the slotted hole 12 that transversely runs through the inside base of connector the latter half, just slotted hole 12 communicate with each other with the little recess 11 on connector both sides limit, just little recess 11 and slotted hole 12's the setting of connector both sides can avoid closely laminating between connecting piece and the cultivation box body and lead to inside the unable entering culture box body of culture solution, can make things convenient for the operator to cultivate the box body internal liquid, provide convenience for the simulation culture work in later stage.
In this embodiment, preferably the top both sides of the first half of connector 7 are provided with elasticity clamping 13, just clamping 13 and the first half fixed connection of connector 7, elasticity clamping 13 adopt the rubber material to make, and have certain toughness and elasticity, and then make things convenient for the top device to get into and take out, also avoid the top device slippage to appear simultaneously, do benefit to and form inclosed atmospheric pressure environment, make things convenient for the doctor to diagnose it and detect.
The use method of the invention comprises the following steps: firstly, placing a connector in a groove B of a culture box body (the height of a cylinder in the groove B and the height of a hollow cylinder in the lower half part of the connector are kept horizontal), then adding a culture solution into the culture box body through the connector, wherein the added culture solution is about 0.5cm higher than a culture surface, then placing the obtained fetal membrane tissue on the culture surface to ensure that the culture solution submerges the fetal membrane tissue, and then placing the fetal membrane tissue into a 37-degree incubator for culture so that the fetal membrane tissue surface is fully attached to the culture surface; after the fetal membrane tissue is fully attached to the culture dish, the top end device is placed in the connector, the fetal membrane tissue and the culture surface are fully closed in a physical mode (the fetal membrane tissue is manually paved on the contact surface and then cultured, and after the fetal membrane tissue cells grow adherent to the wall, the sealing effect is achieved), and meanwhile, the injector can also be used for pressurizing through an elastic porous rubber valve arranged on the top end device, so that the pressure in the uterine cavity is simulated, and the authenticity of experimental data is improved; and when experiment intervention is carried out, the cylinder inside the culture box body is disassembled and taken out, and culture solution or culture solution added with intervention factors is added into the culture box body through the groove outside the connecting body, so that the experiment intervention operation is carried out.
It should be noted that: the invention has simple operation and strong practicability, carries out corresponding experimental intervention by simulating the pressure in the uterine cavity and combining with the real fetal membrane tissue of a human body, thereby comparing, analyzing and knowing whether the premature rupture of the fetal membrane occurs or not, carrying out corresponding molecular biology means for detection after the premature rupture occurs and the like, being beneficial to carrying out various experimental researches while keeping the activity of the fetal membrane, providing a convenient and effective experimental device for the relevant researches of the fetal membrane in obstetrical department, and being suitable for large-scale popularization and application.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and additions can be made without departing from the principle of the present invention, and these should also be considered as the protection scope of the present invention.
Claims (5)
1. A simulation research device for premature rupture of membranes is characterized by comprising a cylindrical culture box body and a culture top cover positioned on the culture body, wherein the culture box body is in threaded connection with the culture top cover; the culture box body is internally provided with a groove A, the groove A is positioned at the upper half part of the culture box body, the lower half part of the culture box body is also provided with a groove B, the middle position of the groove B is provided with a convex block with threads, the convex block is in threaded connection with a detachable cylinder, the top end surface of the detachable cylinder is a paraffin surface, the interior of the culture box body is provided with a connector, the connector is in a convex structure and consists of an upper hollow cylinder and a lower hollow cylinder with different diameters, the upper half part of the connector is embedded in the groove A in the culture box body, the lower half part of the connector is embedded in the groove B of the culture box body, and the cylinder in the groove B is positioned in the connector; still be provided with the top end device in the hollow cylinder of the first half of connector, the top end device be the cylinder structure, its the intermediate position of top end device bottom still is provided with a recess C, recess C and top end device between be concave character type structure, and the top end device has the one end of recess C and sets up down, and the intermediate position of top end device top still is provided with the porose rubber valve of elasticity, just porose rubber valve of elasticity communicate with each other with recess C.
2. The simulation study device for premature rupture of membranes according to claim 1, wherein the diameter of groove A is greater than that of groove B, and the length of the diameter of the groove A is greater than that of the groove B, and the upper half of the connector is located on the solid part of the culture box.
3. The device for simulating the research on the premature rupture of membranes as claimed in claim 1, wherein the height of the cylinder is consistent with the height of the hollow cylinder in the lower half part of the connector.
4. The simulation study device for premature rupture of membranes according to claim 1, wherein the two side edges of the connecting body are provided with small grooves embedded in the two side edges of the connecting body and downward integrally, the small grooves sequentially penetrate through the upper half part and the lower half part of the connecting body from top to bottom, the bottom side edge of the lower half part of the connecting body is further provided with a slotted hole transversely penetrating through the bottom edge of the lower half part of the connecting body, and the slotted hole is communicated with the small grooves on the two side edges of the connecting body.
5. The simulation research device for the premature rupture of membranes according to claim 1, wherein elastic clips are arranged on two sides of the top end of the upper half part of the connecting body, and the clips are fixedly connected with the upper half part of the connecting body.
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| CN202110438269.8A CN113112899B (en) | 2021-04-22 | 2021-04-22 | Simulation research device for premature rupture of fetal membranes |
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| CN202110438269.8A CN113112899B (en) | 2021-04-22 | 2021-04-22 | Simulation research device for premature rupture of fetal membranes |
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| CN113112899A true CN113112899A (en) | 2021-07-13 |
| CN113112899B CN113112899B (en) | 2024-03-22 |
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| JP2016202076A (en) * | 2015-04-22 | 2016-12-08 | 学校法人近畿大学 | Culture apparatus, and cell culture method using culture apparatus |
| CN107603878A (en) * | 2017-09-05 | 2018-01-19 | 广东省第二人民医院 | Cell culture apparatus and its method under a kind of in-vitro simulated pressing environment |
| CN110491234A (en) * | 2019-08-29 | 2019-11-22 | 北京航空航天大学 | A kind of experimental provision of mechanics of biomembranes pressure and mechanical property |
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2021
- 2021-04-22 CN CN202110438269.8A patent/CN113112899B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006212017A (en) * | 2005-02-03 | 2006-08-17 | Otake:Kk | Apparatus for carrying out rotational culture under loading and pressure |
| CN101421388A (en) * | 2006-01-16 | 2009-04-29 | 利莱恩斯生命科学有限公司 | Device for culturing and transporting cells |
| CN202003576U (en) * | 2011-03-21 | 2011-10-05 | 中国人民解放军第四军医大学 | In-vitro placental barrier model |
| CN103740591A (en) * | 2014-01-17 | 2014-04-23 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture device and application thereof |
| JP2016202076A (en) * | 2015-04-22 | 2016-12-08 | 学校法人近畿大学 | Culture apparatus, and cell culture method using culture apparatus |
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