CN113109329A

CN113109329A - Clinical test sample kit convenient for comparison and use method thereof

Info

Publication number: CN113109329A
Application number: CN202110375190.5A
Authority: CN
Inventors: 田辉; 孙振国; 岳韦名; 李树海; 张慧颖
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-04-08
Filing date: 2021-04-08
Publication date: 2021-07-13
Anticipated expiration: 2041-04-08
Also published as: CN113109329B

Abstract

The invention discloses a clinical test sample kit convenient for comparison and a use method thereof, belonging to the field of kits, wherein the clinical test sample kit convenient for comparison comprises a detection piece and an observation piece rotationally connected to the top end of the detection piece through a rotating shaft, the observation piece comprises a first shell and a second shell which are connected in an involutory manner, one opposite sides of the first shell and the second shell are respectively provided with an insertion groove, a reaction plate matched with the insertion groove is arranged in the insertion groove, a conduit is arranged in the detection piece, the bottom of the detection piece is provided with a detection bin, the top end of the inner wall of the detection bin is fixedly provided with a cylindrical connecting piece, the central axis of the cylindrical connecting piece is provided with a through hole, and a collecting needle is sleeved in the through hole.

Description

Clinical test sample kit convenient for comparison and use method thereof

Technical Field

The invention relates to the field of kits, in particular to a clinical test sample kit convenient for comparison and a using method thereof.

Background

The kit is generally used for containing boxes for detecting chemical reagents such as chemical components, drug residues, virus types and the like, and is frequently used in general hospitals, pharmaceutical enterprises and chemical plants. However, the general kit has a single structure, only can store a single reagent, mainly plays a role in storage, and generally needs to go to a special laboratory when detection is needed, but in a first-line production research and development department of a chemical plant, for the frequent condition of clinical tests, rapid detection is needed and sample attributes are compared, so the general kit cannot meet actual requirements.

Esophageal cancer is one of common malignant tumors in China, and the main type of esophageal cancer in China is esophageal squamous cell carcinoma. In recent years, multidisciplinary comprehensive treatment improves the treatment effect of squamous cell carcinoma to a certain extent, but the overall curative effect is still to be improved, and the 5-year survival rate is still low. Therefore, the occurrence and development mechanisms of squamous carcinoma are deeply disclosed, and new biomarkers are developed, which have important significance for auxiliary diagnosis and typing of esophageal carcinoma and improvement of treatment of esophageal carcinoma patients. The gene with the highest methylation frequency in squamous cell carcinoma is MGMT. MGMT is a DNA repair enzyme that removes methyl or alkyl groups from guanine, thereby protecting cells from guanine to adenine mutations. Abnormal hypermethylation of MGMT is associated with lymph node metastasis in squamous cell carcinoma patients and with the folate metabolizing enzyme gene MTHFR C677T polymorphism. In squamous cell carcinoma cell lines, inhibitor treatment of DNA methyltransferase DNMT can partially reverse the CpG hypermethylation state and restore mRNA expression of the MGMT gene, indicating that MGMT hypermethylation is a key mechanism for its silencing, and that loss of MLH1 protein is negatively correlated with its promoter hypermethylation as found in squamous cell carcinoma.

With the progress of scientific research, how to quickly detect and compare the MGMT content in blood clinically can visually display the hypermethylation degree, and has direct significance for preventing and judging esophageal squamous cell carcinoma, but the existing MGMT methylation kit needs to be operated by professional staff during detection, has complex detection steps, consumes a large amount of manpower for comparison and detection, and has high detection cost, so that a clinical test sample kit convenient for comparison and a using method thereof are provided for solving the problems.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the invention aims to provide a clinical test sample kit convenient for comparison and a using method thereof, when in use, the detecting piece and the observing piece are bent to be ninety degrees, the extrusion air bag and the conduit are extruded by the arc groove and the bottom end of the observing piece to discharge the air inside, the extrusion block enters the capsule bin from the arc leading-in opening and presses the reagent capsule and the wall breaking needle to break the reagent capsule while the detecting piece and the observing piece are bent, at the moment, the sealing tinfoil is uncovered, the finger to be detected extends into the detecting bin and extrudes the flexible sucker and the collecting needle, a closed space is formed between the flexible sucker and the finger, the collecting needle overcomes the elasticity of the tensioning spring while the finger is punctured by the force, the sealing gasket is far away from the liquid guiding through hole, the bending detecting piece and the observing piece are recovered, the collected blood flows into the conduit from the flexible sucker by utilizing the negative pressure state of the conduit and reacts with the reaction plate, meanwhile, reagents in the reagent capsules flow into the reaction plate to be diluted, the device is simple in structure and easy and convenient to operate, can be operated without the guidance of professionals, can effectively improve the contrast efficiency, has a market prospect, and is suitable for popularization.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

A clinical test sample kit convenient for comparison comprises a detection piece and an observation piece rotatably connected to the top end of the detection piece through a rotating shaft, wherein the observation piece comprises a first shell and a second shell which are connected in an involutive manner, one opposite side of the first shell and the second shell is provided with an insertion groove, a reaction plate matched with the insertion groove is arranged in the insertion groove, a guide pipe is arranged in the detection piece, the bottom of the detection piece is provided with a detection bin, the top end of the inner wall of the detection bin is fixedly provided with a cylindrical connecting piece, the central axis of the cylindrical connecting piece is provided with a through hole, a collection needle is sleeved in the through hole, a tension spring is fixed between the outer wall of the collection needle and the inner wall of the through hole of the cylindrical connecting piece, the bottom end of the cylindrical connecting piece is fixedly provided with a flexible sucker, the top end of the cylindrical connecting piece is communicated with the bottom, the liquid guide through hole is communicated with the guide pipe and the flexible sucker, a sealing gasket matched with the liquid guide through hole is fixed at the bottom end of the nail cap of the collecting needle, the connector is fixed at the bottom of the insertion groove, the top end of the guide pipe is communicated with the bottom end of the connector, an arc groove is formed in the top end of the detection piece, and an extrusion air bag is fixed on the outer wall of the guide pipe. The collecting needle overcomes the elasticity of the tension spring when the finger is punctured by stress, so that the sealing gasket is far away from the liquid guide through hole, the bending detection piece and the observation piece are recovered, collected blood flows into the guide pipe from the flexible sucker and reacts with the reaction plate by utilizing the negative pressure state of the guide pipe, and meanwhile reagent in the reagent capsule flows into the reaction plate to be diluted.

Furthermore, a capsule bin is arranged on one side of the first shell opposite to the reaction plate, the capsule bin is communicated with the insertion groove, reagent capsules are installed in the capsule bin, a baffle is fixed on one side of the capsule bin opposite to the reaction plate, a wall breaking needle is fixed on one side of the baffle opposite to the reagent capsules, an extrusion block is fixed on one side of the detection piece, the extrusion block is of an arc-shaped block structure, an arc-shaped lead-in port matched with the extrusion block is arranged on one side of the capsule bin far away from the reaction plate, the arc-shaped lead-in port and a rotating shaft of the extrusion block are coaxially arranged with a rotating shaft connected with the observation piece, the reagent capsules are pressed against the wall breaking needle when the bending detection piece and the observation piece are ninety degrees, so that the reagent capsules are damaged, and the discharge amount of the reagent in the reagent capsules can be controlled by increasing the bending number and the angle of the bending detection piece and the observation, the operation is convenient.

Further, the inboard that detects the storehouse is toper empty storehouse structure, the inner wall that detects the storehouse is fixed with a plurality of clamping rings, the clamping ring is silica gel elasticity clamping ring structure, the bottom that detects the storehouse is spliced and is equipped with sealed tinfoil, designs for toper empty storehouse structure through the inboard that will detect the storehouse, can accord with the user demand that different points to at the bulkhead design clamping ring that detects the storehouse, when the clamping ring contacts with the finger, promoted the seal that detects the storehouse and increased the amount of filling blood of finger simultaneously, promoted to see the collection and examine the amount of bleeding of time measuring.

Furthermore, the reaction plate is of a structure with a wide upper part and a narrow lower part, the bottom end of the reaction plate is of a cylindrical structure, the radius of the bottom end of the reaction plate is equal to the diameter of the inner wall of the connector, and the reaction plate is inserted from the top of the insertion groove and is in butt joint with the connector through the structural design of the structure with the wide upper part and the narrow lower part and the cylindrical bottom of the reaction plate, so that the assembly efficiency is improved, and the manufacturing difficulty is reduced.

Further, flexible sucking disc is bowl form silica gel structure, the tensioning spring has the drive the elastic force that the nut of gathering the needle is close to the cylindricality connecting piece, it is located flexible sucking disc's lower terminal surface to gather its bottom extreme point under free state, flexible sucking disc is greater than the biggest stroke volume of gathering the needle and the two difference of the upward displacement of collection needle and is 0.1-0.3CM at vertical direction pressurized deformation volume, and through flexible sucking disc's design, make the finger when pressing the collection needle, can not press too deeply and be injured, can form airtight space at the finger tip simultaneously, the collection of the blood of being convenient for.

Further, the bottom of the observation piece is of an arc-shaped structure taking a rotating shaft connected with the observation piece as a rotating shaft, the rotating shaft of the arc-shaped groove is coaxial with the rotating shaft connected with the observation piece, the distance between the arc-shaped groove and the bottom of the observation piece is equal to the total wall thickness of the extrusion air bag and the catheter, the arc-shaped groove and the bottom of the observation piece rotate relatively through the bending detection piece and the observation piece, so that the extrusion air bag and the catheter are extruded to discharge internal air to form a negative pressure state, when the bending detection piece and the observation piece reset, the extrusion air bag and the catheter start to reset, the collection needle does not reset at the moment, the pressure in the catheter is smaller than the pressure between the flexible sucker and a finger, blood flows into the catheter from the flexible sucker, when the finger is taken out, the collection needle resets, the bottom.

Further, carry out detachable closing through the buckle between first casing and the second casing, first casing, second casing and detection piece are medical soluble polytetrafluoroethylene integrated into one piece's injection moulding structure, through the adoption of soluble polytetrafluoroethylene material and injection moulding process, have promoted manufacturing efficiency and safety in utilization ability.

Further, the outer wall one end of first casing is equipped with the viewing aperture, viewing aperture and inserting groove intercommunication set up, one side of viewing aperture is equipped with contrast color card, through the structural design of viewing aperture and contrast color card, has promoted contrast efficiency and contrast directly perceived nature.

Furthermore, the reaction plate is coated with an antibody protein coating matched with an MGMT antigen on the surface, the antibody protein coating is an enzyme-labeled antibody, and the filling liquid in the reagent capsule is a sample diluent and is suitable for MGMT detection in esophageal squamous cell carcinoma.

A method of using a clinical test sample kit for facilitating comparison, comprising the steps of:

s1, sterilizing both hands of a user, taking out the kit from the sterile packaging bag, and placing the kit at room temperature for balancing for 15-19 minutes;

s2, disinfecting the finger to be detected again, bending the detection piece and the observation piece to ninety degrees, tearing the sealing tinfoil, extending the finger to be detected into the detection bin, and extruding the flexible sucker and the acquisition needle;

s3, repeatedly bending the detection piece and the observation piece for multiple times;

s4, restoring the bending detection piece and the observation piece, taking out the finger to be detected, and waiting for 5-10 minutes to read the result from the contrast color card at the observation port.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

(1) when the scheme is used, the detection piece and the observation piece are bent by ninety degrees, the extrusion air bag and the guide pipe are extruded by the arc groove and the bottom end of the observation piece to discharge the internal air, the extrusion block enters the capsule bin from the arc introducing port while bending the detection piece and the observation piece and presses the reagent capsule and the wall breaking needle to be extruded, so that the reagent capsule is damaged, the sealing tinfoil is uncovered, the finger to be detected extends into the detection bin and extrudes the flexible sucking disc and the acquisition needle, a closed space is formed between the flexible sucking disc and the finger, the elastic force of the tensioning spring is overcome while the finger is punctured by the acquisition needle, the sealing pad is far away from the liquid guiding through hole, the bending detection piece and the observation piece are restored, the acquired blood flows into the guide pipe from the flexible sucking disc and reacts with the reaction plate by utilizing the negative pressure state of the guide pipe, and the reagent in the reagent capsule flows out of the reaction plate to carry, the invention has simple structure and simple and convenient operation, can be operated without the guidance of professionals, can effectively improve the contrast efficiency, has market prospect and is suitable for popularization.

(2) When being ninety degrees through buckling the testing part and observing the piece, oppression reagent capsule extrudees with broken wall needle, makes the reagent capsule damaged to buckle the emission of this number of buckling and the controllable reagent capsule of angle of testing part and observing the piece through the promotion, the simple operation.

(3) The inboard design through will detecting the storehouse is toper empty storehouse structure, can accord with the user demand that different points to at the bulkhead design clamping ring that detects the storehouse, when the clamping ring contacted with the finger, promoted the seal that detects the storehouse and increased the amount of filling blood of finger simultaneously, promote to see the amount of bleeding that gathers detection time measuring.

(4) Through the structural design of the structure that the reaction plate is wide at the top and narrow at the bottom and is cylindrical at the bottom, the reaction plate is convenient to insert from the top of the inserting groove and is butted with the connector during production and manufacturing, the assembly efficiency is improved, and the manufacturing difficulty is reduced.

(5) Through the design of flexible sucking disc, make the finger when pressing the collection needle, can not press too deeply and be injured, can form airtight space, the collection of the blood of being convenient for at finger tip simultaneously.

(6) Through buckling the detection piece and observing the piece, relative rotation between arc groove and observation piece bottom to extrusion gasbag and pipe receive the inside air of extrusion discharge, form negative pressure state, when buckling the detection piece and observing the piece and reseing, extrusion gasbag and pipe begin to reset, gather the needle and do not reset this moment, pressure in the pipe is less than the pressure between flexible sucking disc and finger, blood flows into in the pipe from flexible sucking disc, take out when the finger, gather the needle and reset, it seals to flow into the pipe bottom, accomplish the blood sampling process.

(7) By adopting the soluble polytetrafluoroethylene material and the injection molding process, the manufacturing efficiency and the use safety performance are improved.

(8) Through the structural design of viewing aperture and contrast colour chip, promoted contrast efficiency and contrast directly perceived nature.

(9) Is suitable for MGMT detection in esophageal squamous cell carcinoma.

Drawings

FIG. 1 is a schematic front view of the present invention;

FIG. 2 is a schematic bottom view of the present invention;

FIG. 3 is a schematic cross-sectional view of the present invention;

FIG. 4 is an enlarged view of portion A of FIG. 3;

FIG. 5 is an enlarged view of the portion B in FIG. 3;

FIG. 6 is an enlarged view of the portion C of FIG. 3;

FIG. 7 is a schematic illustration of an explosive structure according to the present invention;

FIG. 8 is a schematic view of an exploded structure of the cylindrical connector and the conduit according to the present invention;

fig. 9 is a schematic view of an internal structure of a first housing proposed in the present invention;

FIG. 10 is a schematic view of the present invention in use bent ninety degrees;

FIG. 11 is a flow chart of a method of use of the present invention.

The reference numbers in the figures illustrate:

1, 101, 102, a plug groove, 103, 11 an observation port, 12 contrast color cards, 13 capsule bins, 131 baffles, 132 wall breaking needles, 14 reagent capsules, 2 detection pieces, 21 extrusion blocks, 22 detection bins, 221 sealing tinfoil, 222 compression rings, 23 arc grooves, 3 reaction plates, 4 catheters, 41 extrusion air bags, 5 cylindrical connecting pieces, 51 flexible suction cups, 52 acquisition needles, 53 tensioning springs, 54 liquid guiding through holes and 55 sealing gaskets.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1:

referring to fig. 1 and 7, a clinical test sample kit convenient for comparison comprises a detection part 2 and an observation part rotatably connected to the top end of the detection part 2 through a rotating shaft, the observation part comprises a first shell 1 and a second shell 101 which are connected in an involutory manner, one side of the first shell 1 opposite to the second shell 101 is provided with a splicing groove 102, a reaction plate 3 matched with the splicing groove 102 is arranged in the splicing groove 102, one end of the outer wall of the first shell 1 is provided with an observation port 11, the observation port 11 is communicated with the splicing groove 102, one side of the observation port 11 is provided with a contrast color card 12, through the structural design of the observation port 11 and the contrast color card 12, the comparison efficiency and the comparison intuitiveness are improved, the first shell 1 and the second shell 101 are detachably involutory through a buckle, the first shell 1, the second shell 101 and the detection part 2 are all injection molding structures integrally formed by medical soluble polytetrafluoroethylene, by adopting the soluble polytetrafluoroethylene material and the injection molding process, the manufacturing efficiency and the use safety performance are improved.

Referring to fig. 4 and 9, a capsule chamber 13 is disposed on a side of the first housing 1 opposite to the reaction plate 3, the capsule chamber 13 is communicated with the insertion groove 102, a reagent capsule 14 is installed in the capsule chamber 13, a baffle 131 is fixed on a side of the capsule chamber 13 opposite to the reaction plate 3, a wall breaking needle 132 is fixed on a side of the baffle 131 opposite to the reagent capsule 14, an antibody protein coating matched with an MGMT antigen is coated on the surface of the reaction plate 3, the antibody protein coating is an enzyme-labeled antibody, and a filling liquid in the reagent capsule 14 is a sample diluent, which is suitable for MGMT detection in esophageal squamous cell carcinoma.

Referring to fig. 5, an extrusion block 21 is fixed on one side of the detection member 2, the extrusion block 21 is an arc block structure, an arc-shaped introduction port matched with the extrusion block 21 is arranged on one side of the capsule chamber 13 away from the reaction plate 3, the arc-shaped introduction port and a rotation shaft of the extrusion block 21 are coaxially arranged with a rotation shaft connected with the observation member, the reagent capsule 14 is pressed to be extruded with the wall breaking needle 132 when the bending detection member 2 and the observation member are ninety degrees, the reagent capsule 14 is damaged, the discharge amount of the reagent in the reagent capsule 14 can be controlled by increasing the bending number and the bending angle of the bending detection member 2 and the observation member, and the operation is convenient.

Referring to fig. 5 and 6, a conduit 4 is arranged in a detection member 2, a detection chamber 22 is arranged at the bottom of the detection member 2, a cylindrical connecting member 5 is fixed at the top end of the inner wall of the detection chamber 22, a through hole is arranged on the central axis of the cylindrical connecting member 5, a collection needle 52 is sleeved in the through hole, a tension spring 53 is fixed between the outer wall of the collection needle 52 and the inner wall of the through hole of the cylindrical connecting member 5, a flexible suction cup 51 is fixed at the bottom end of the cylindrical connecting member 5, the top end of the cylindrical connecting member 5 is communicated with the bottom end of the conduit 4, a liquid guide through hole 54 is arranged on the cylindrical connecting member 5, the conduit 4 is communicated with the flexible suction cup 51 through the liquid guide through hole 54, a sealing gasket 55 matched with the liquid guide through hole 54 is fixed at the bottom end of the collection needle 52, a connector 103 is fixed at the bottom of an insertion groove 102, the reaction plate 3 is of, through the structural design of the reaction plate 3 with a cylindrical structure with a wide upper part and a narrow lower part and a cylindrical bottom, the reaction plate 3 is inserted from the top of the insertion groove 102 and is in butt joint with the connector 103 when the invention is produced and manufactured, the assembly efficiency is improved, and the manufacturing difficulty is reduced.

Referring to fig. 5 and 8, the top end of the conduit 4 is communicated with the bottom end of the connector 103, the top end of the detecting member 2 is provided with an arc-shaped groove 23, the outer wall of the conduit 4 is fixed with an extruding airbag 41, when in use, the detecting member 2 and the observing member are bent by ninety degrees, at this time, the extruding airbag 41 and the conduit 4 are extruded by the arc-shaped groove 23 and the bottom end of the observing member to discharge the internal air, while the detecting member 2 and the observing member are bent, the extruding block 21 enters the capsule chamber 13 from the arc-shaped inlet and presses the reagent capsule 14 and the wall breaking needle 132 to damage the reagent capsule 14, at this time, the sealing tinfoil 221 is uncovered, the finger to be detected is extended into the detecting chamber 22 and extrudes the flexible suction cup 51 and the collecting needle 52, a closed space is formed between the flexible suction cup 51 and the finger, the collecting needle 52 is stressed to puncture the finger, and overcomes the elasticity of the tension spring 53, and, at the moment, the bending detection piece 2 and the observation piece are restored, collected blood flows into the guide tube 4 from the flexible sucker 51 and reacts with the reaction plate 3 by utilizing the negative pressure state of the guide tube 4, and meanwhile, reagents in the reagent capsules 14 flow into the reaction plate 3 to be diluted.

Please refer to fig. 3, the inner side of the detection chamber 22 is a tapered empty chamber structure, the inner wall of the detection chamber 22 is fixed with a plurality of press rings 222, the press rings 222 are silica gel elastic press ring structures, the bottom end of the detection chamber 22 is connected with the sealing tinfoil 221, the inner side of the detection chamber 22 is designed into the tapered empty chamber structure, which can meet the use requirements of different fingers, and the press rings 222 are designed on the chamber wall of the detection chamber 22, when the press rings 222 contact with the fingers, the sealing performance of the detection chamber 22 is improved, the blood charging amount of the fingers is increased at the same time, and the blood output amount during the collection and detection is improved.

Referring to fig. 6, the flexible suction cup 51 is a bowl-shaped silica gel structure, the tension spring 53 has an elastic force for driving the nail cap of the collection needle 52 to approach the cylindrical connecting member 5, the bottom end point of the collection needle 52 is located on the lower end surface of the flexible suction cup 51 in a free state, the compression deformation amount of the flexible suction cup 51 in the vertical direction is greater than the maximum stroke amount of the upward displacement of the collection needle 52, and the difference between the two is 0.1 CM to 0.3CM, so that when the collection needle 52 is pressed by a finger, the finger cannot be pressed too deep to be injured, and meanwhile, a closed space can be formed at the end of the finger, thereby facilitating the collection of blood.

Referring to fig. 1-10, the bottom end of the observation piece is an arc-shaped structure using a rotating shaft connected with the observation piece as a rotating shaft, the rotating shaft of the arc-shaped groove 23 and the rotating shaft connected with the observation piece are coaxially arranged, the distance between the arc-shaped groove 23 and the bottom end of the observation piece is equal to the total wall thickness of the extrusion air bag 41 and the catheter 4, the arc-shaped groove 23 and the bottom end of the observation piece relatively rotate through the bending detection piece 2 and the observation piece, so that the extrusion air bag 41 and the catheter 4 are extruded to discharge internal air to form a negative pressure state, when the bending detection piece 2 and the observation piece are reset, the extrusion air bag 41 and the catheter 4 start to reset, at this time, the collection needle 52 is not reset, the pressure in the catheter 4 is smaller than the pressure between the flexible sucker 51 and the finger, blood flows into the catheter 4 from the flexible sucker 51, when the finger is taken out.

Referring to fig. 11, a method for using a clinical test sample kit for facilitating comparison includes the following steps:

s2, the finger to be detected is disinfected again, the detection piece 2 and the observation piece are bent for ninety degrees, the sealing tinfoil 221 is torn at the moment, the finger to be detected is inserted into the detection bin 22, and the flexible suction disc 51 and the acquisition needle 52 are squeezed;

s3, repeatedly bending the detection piece 2 and the observation piece for multiple times;

s4, restoring the bending detection piece 2 and the observation piece, taking out the finger to be detected, and waiting for 5-10 minutes to interpret the result from the contrast color card 12 of the observation port 11.

The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.

Claims

1. The utility model provides a clinical trial sample kit convenient to contrast, includes and detects piece (2) and rotates the observation piece of connection in detection piece (2) top through the pivot, its characterized in that: the observation piece comprises a first shell (1) and a second shell (101) which are connected in an involutory manner, wherein insertion grooves (102) are formed in one opposite sides of the first shell (1) and the second shell (101), reaction plates (3) matched with the insertion grooves (102) are arranged in the insertion grooves (102), a guide pipe (4) is arranged in the detection piece (2), a detection bin (22) is arranged at the bottom of the detection piece (2), a cylindrical connecting piece (5) is fixed at the top end of the inner wall of the detection bin (22), a through hole is formed in the central axis of the cylindrical connecting piece (5), a collection needle (52) is sleeved in the through hole, a tensioning spring (53) is fixed between the outer wall of the collection needle (52) and the inner wall of the through hole of the cylindrical connecting piece (5), a flexible sucker (51) is fixed at the bottom end of the cylindrical connecting piece (5), and the top end of the guide pipe (4) is communicated with each other, be equipped with drain through hole (54) on cylindricality connecting piece (5), drain through hole (54) intercommunication pipe (4) and flexible sucking disc (51), the nut bottom mounting of gathering needle (52) have with drain through hole (54) assorted sealed pad (55), the bottom of inserting groove (102) is fixed with connector (103), the top and connector (103) bottom intercommunication setting of pipe (4), the top of detecting part (2) is equipped with arc groove (23), the outer wall of pipe (4) is fixed with extrusion gasbag (41).

2. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: the reagent capsule detection device is characterized in that a capsule bin (13) is arranged on one side, opposite to the reaction plate (3), of the first shell (1), the capsule bin (13) is communicated with the insertion groove (102), a reagent capsule (14) is installed in the capsule bin (13), a baffle (131) is fixed on one side, opposite to the reaction plate (3), of a bin opening of the capsule bin (13), a wall breaking needle (132) is fixed on one side, opposite to the reagent capsule (14), of the baffle (131), an extrusion block (21) is fixed on one side of the detection piece (2), the extrusion block (21) is of an arc-shaped block structure, an arc-shaped introducing port matched with the extrusion block (21) is arranged on one side, far away from the reaction plate (3), of the capsule bin (13), and rotating shafts of the arc-shaped introducing port and the extrusion block (21) are coaxially arranged with a rotating shaft connected with.

3. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: the inboard that detects storehouse (22) is toper empty storehouse structure, the inner wall that detects storehouse (22) is fixed with a plurality of clamping rings (222), clamping ring (222) are silica gel elasticity clamping ring structure, the bottom that detects storehouse (22) splices and is equipped with sealed tinfoil (221).

4. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: the reaction plate (3) is of a structure with a wide upper part and a narrow lower part, the bottom end of the reaction plate (3) is of a cylindrical structure, and the radius of the bottom end of the reaction plate (3) is equal to the diameter of the inner wall of the connector (103).

5. A clinical trial sample kit for facilitating comparison and method of use thereof according to claim 1, wherein: flexible sucking disc (51) are bowl form silica gel structure, tensioning spring (53) have the drive the nut of gathering needle (52) is close to the elasticity of cylindricality connecting piece (5), gather needle (52) its bottom extreme point under free state and be located the lower terminal surface of flexible sucking disc (51), flexible sucking disc (51) is greater than the biggest stroke volume and the two difference of gathering needle (52) displacement upwards at vertical direction compression deformation volume and is 0.1-0.3 CM.

6. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: the bottom of observation piece is for using the pivot of connecting the observation piece as the circular-arc structure of rotation axis, the rotation axis of arc groove (23) and the coaxial setting of pivot of connecting the observation piece, the interval of arc groove (23) and observation piece bottom equals the total wall thickness of extrusion gasbag (41) and pipe (4).

7. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: the medical soluble polytetrafluoroethylene detection device is characterized in that the first shell (1) and the second shell (101) are detachably closed through buckles, and the first shell (1), the second shell (101) and the detection piece (2) are all injection molding structures integrally formed by medical soluble polytetrafluoroethylene.

8. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: an observation port (11) is arranged at one end of the outer wall of the first shell (1), the observation port (11) is communicated with the insertion groove (102), and a contrast color card (12) is arranged on one side of the observation port (11).

9. A clinical trial sample kit for facilitating comparison according to claim 1, wherein: the reaction plate (3) is coated with an antibody protein coating matched with MGMT antigen on the surface, the antibody protein coating is an enzyme-labeled antibody, and the filling liquid in the reagent capsule (14) is sample diluent.

10. The method for using the clinical test sample kit for facilitating comparison according to claim 1, comprising the steps of:

s2, the finger to be detected is disinfected again, the detection piece (2) and the observation piece are bent for ninety degrees, the sealing tinfoil (221) is torn at the moment, the finger to be detected is extended into the detection bin (22), and the flexible sucker (51) and the collecting needle (52) are squeezed;

s3, repeatedly bending the detection piece (2) and the observation piece for multiple times;

s4, the recovery bending detection piece (2) and the observation piece, the finger to be measured is taken out, and the result is read from the contrast color card (12) of the observation port (11) after waiting for 5-10 minutes.