CN113106024A - Stem cell culture vessel - Google Patents

Stem cell culture vessel Download PDF

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Publication number
CN113106024A
CN113106024A CN202110556241.4A CN202110556241A CN113106024A CN 113106024 A CN113106024 A CN 113106024A CN 202110556241 A CN202110556241 A CN 202110556241A CN 113106024 A CN113106024 A CN 113106024A
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circular
shaped
block
groove
annular
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CN202110556241.4A
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Chinese (zh)
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孙海艳
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/54Constructional details, e.g. recesses, hinges hand portable
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a stem cell culture vessel, which comprises a circular ring shell and a cover body, wherein a circular base is fixedly arranged at the bottom of the circular ring shell, a limiting device is arranged in the circular ring shell at the center of the top of the circular base, the invention comprises a circular ring shell, a circular base, a limiting device, a separating device and a cover body, wherein the top of the circular base is uniformly provided with the separating device in the circular ring shell, the cover body is arranged above the circular ring shell, the separating device is firstly placed in the circular ring shell on the circular base according to different types of cultured cells and then is limited by the limiting device, thereby make the suitability reinforcing when cultivateing cell, lock lid and ring housing fast at last, avoided traditional rotatory locking of screw thread, and can not influence the stability of cell in the culture dish.

Description

Stem cell culture vessel
Technical Field
The invention relates to the field of culture dishes, in particular to a stem cell culture dish.
Background
A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types of materials, mainly plastic and glass, the glass can be used for plant materials, microorganism culture and adherent culture of animal cells, and the plastic can be made of polyethylene materials, is disposable and reusable, is suitable for operations of laboratory inoculation, streaking and bacterium separation, and can be used for culture of plant materials. Culture dishes are commonly used to culture stem cells, a type of cell that has unlimited or immortal self-renewal capacity, and is capable of producing at least one type of highly differentiated progeny cells.
In the process of culturing cells, due to the limitation of the culture dish, the following problems exist in the practical operation process:
when stem cell cultivates, a culture dish can only cultivate a cell, and when multiple cell was cultivateed to needs, required culture dish quantity also can be more, leads to constant temperature culture room storage pressure great, and current cell culture dish also has two regional culture dishes, nevertheless when extracting the cell in one of them region, pollutes another region because of the hand is trembled very easily, can not be alone with this regional whole taking out, and the use can cause inconveniently.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a stem cell culture vessel.
The technical scheme adopted by the invention to solve the technical problem is as follows: a stem cell culture vessel comprises a circular ring shell and a cover body, wherein a circular base is fixedly installed at the bottom of the circular ring shell, a limiting device is arranged in the circular ring shell at the center of the top of the circular base, a separating device is uniformly arranged in the circular ring shell at the top of the circular base, and the cover body is arranged above the circular ring shell;
the limiting device comprises a cylindrical block, a wedge block, a first spline groove, a spline rod, an annular block, a disc block, a second spline groove, an annular limiting plate, a circular head plate and a compression spring, wherein the cylindrical block is fixedly arranged at the top of the circular base, clamping grooves are uniformly formed in the top of the cylindrical block along the circumferential direction, the wedge block is arranged in the clamping grooves, the inclination angle of the wedge block is gradually reduced from top to bottom, the first spline groove is formed in the center of the top of the cylindrical block, the spline rod is arranged in the first spline groove, the annular block is fixedly arranged at the outer side of the spline rod at the top of the cylindrical block, the disc block is arranged at the top of the annular block, the disc block is in a petal shape, the second spline groove is formed in the top of the disc block, the spline rod penetrates through the second spline groove to extend upwards, the annular limiting plate is fixedly arranged at the outer side of the disc block at the top of the cylindrical block, a round head plate is arranged in the sliding hole in a sliding manner, one end, positioned on the outer side of the annular limiting plate, of the bottom of the round head plate is inclined, the thickness of the tail end of the bottom of the round head plate is gradually reduced from the center of the cylindrical block to the edge, a compression spring is arranged on the round head plate, one end of the compression spring is fixedly connected with the inner wall of the annular limiting plate, and the other end of the compression spring is arranged on the round head plate;
the separating device comprises fan-shaped separating plates, fan-shaped grooves, rubber pads and clamping blocks, wherein a plurality of groups of fan-shaped separating plates are uniformly arranged on the top of the circular base in the circular ring shell, the fan-shaped grooves are formed in the top of the fan-shaped separating plates, the rubber pads are fixedly arranged at one ends of the fan-shaped separating plates, which are positioned on the inner wall of the circular ring shell, the clamping blocks are fixedly arranged at the other ends of the fan-shaped separating plates, and the clamping blocks are positioned in the clamping grooves;
the cover body comprises a circular cover, an annular hollow cylinder, a Contraband-shaped block, clamping grooves, displacement grooves, L-shaped clamping plates and a telescopic spring rod, the bottom of the circular cover is fixedly provided with the annular hollow cylinder, the annular hollow cylinder is matched with the annular shell, the inner side wall of the annular hollow cylinder is provided with a V-21274, the outer side wall of the annular hollow cylinder is located at the V-21274, the clamping grooves are formed in the positions of the V-21274, the displacement grooves are formed in vertical sections at two ends of the V-21274, the clamping grooves are internally provided with two sets of L-shaped clamping plates, and the vertical sections of the two sets of L-shaped clamping plates are connected through the telescopic spring rod.
As a preferred technical scheme, the left side wall and the right side wall of the circular shell are provided with a first rectangular groove, and the front end surface and the rear end surface of the first rectangular groove are provided with positioning grooves.
As a preferred technical scheme of the invention, the inner side wall of the circular shell is uniformly provided with a first arc-shaped groove along the circumferential direction; when stop device makes the rubber pad on the fan-shaped division board and the inner wall of ring casing contact, an arc recess can increase frictional force for the inside wall laminating of rubber pad and ring casing is inseparabler.
As a preferred technical scheme of the invention, the bottom of the disc block is provided with an annular groove, and the top of the annular block is positioned in the annular groove; the annular groove is formed in the bottom of the disc block, so that the disc block is supported by the annular block, and the rotation of the disc block is not influenced.
As a preferred technical scheme of the invention, the front side and the rear side of the side wall of the circular shell are symmetrically and fixedly provided with a v-shaped handle 21274; through the arranged v-21274', the shape handle is convenient to take, and the corners of the v-21274-shaped handle are arc-shaped, so that the appearance is attractive, and the potential safety hazard is avoided.
As a preferred technical scheme, a handle is fixedly arranged in the middle of the top of the circular cover, a first circular groove is formed in the bottom of the handle, a second circular groove is formed in the center of the bottom of the circular cover, and the second circular groove is communicated with the first circular groove; the cover is convenient to take through the installed handle, and the spline rod is convenient to store through the arranged first round groove and the second round groove.
As a preferred technical scheme, a first pull ring is uniformly arranged on the outer side wall of the annular limiting plate along the circumferential direction, a second pull ring is arranged at one end, close to the annular limiting plate, of the wedge block, and the first pull ring is connected with the second pull ring through an anti-lost rope; through the rope of losing of preventing that sets up, prevent that the wedge from losing because of the structural constraint of wedge, be difficult for seeking.
As a preferred technical scheme of the invention, one end of the clamping block close to the side wall of the cylindrical block is inclined, and the inclination angle is gradually increased from top to bottom; through the chucking piece of slope, when the wedge promoted the chucking piece and make fan-shaped division board and the inner wall contact of ring casing, the inclined plane of wedge just cooperated with the inclined plane of chucking piece.
Compared with the prior art, the invention has the following advantages:
1. according to the invention, through the arrangement of the circular ring shell, the circular base, the limiting device, the separating device and the cover body, the separating device is firstly placed in the circular ring shell on the circular base according to different types of cultured cells and then limited by the limiting device, so that the applicability of the cell culture is enhanced, and finally the cover body and the circular ring shell are quickly locked, so that the traditional locking of screw rotation is avoided, and the stability of the cells in the culture dish is not influenced.
2. According to the invention, the v-shaped handle 21274is convenient to take, and the corners of the v-shaped handle 21274are arc-shaped, so that the appearance is attractive, and the potential safety hazard is avoided.
3. According to the invention, the first arc-shaped groove is uniformly formed in the inner side wall of the circular ring shell, so that when the limiting device enables the rubber pad on the fan-shaped isolation plate to be in contact with the inner wall of the circular ring shell, the first arc-shaped groove can increase friction force, and the rubber pad is attached to the inner side wall of the circular ring shell more tightly.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic perspective view of the ring housing, circular base, separating device and limiting device of the present invention;
FIG. 2 is a schematic perspective view of the cover of the present invention;
FIG. 3 is a schematic perspective view of the present invention;
FIG. 4 is a schematic front view of the ring housing, the circular base, the separating device and the limiting device of the present invention;
FIG. 5 is a schematic top view of the ring housing, circular base, spacer and retainer of the present invention;
FIG. 6 is a schematic top view of the spacing device of the present invention;
FIG. 7 is a schematic structural view of the cover of the present invention;
FIG. 8 is a schematic perspective view of the spacing device of the present invention;
fig. 9 is a partial enlarged view of the invention at M of fig. 4.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the effects of the invention easy to understand, the invention is further explained with reference to fig. 1 to 9.
A stem cell culture vessel comprises a circular shell 1 and a cover body 2, wherein a circular base 3 is fixedly installed at the bottom of the circular shell 1, a limiting device 4 is arranged in the circular shell 1 and at the center of the top of the circular base 3, separating devices 5 are uniformly arranged in the circular shell 1 and at the top of the circular base 3, and the cover body 2 is arranged above the circular shell 1;
the limiting device 4 comprises a cylindrical block 4a, a wedge block 40, a first spline groove 41, a spline rod 42, an annular block 43, a disc block 44, a second spline groove 45, an annular limiting plate 46, a round head plate 47 and a compression spring 48, the cylindrical block 4a is fixedly arranged at the top of the circular base 3, clamping grooves are uniformly formed in the top of the cylindrical block 4a along the circumferential direction, the wedge block 40 is arranged in the clamping grooves, the inclination angle of the wedge block 40 is gradually reduced from top to bottom, the first spline groove 41 is formed in the center position of the top of the cylindrical block 4a, the spline rod 42 is arranged in the first spline groove 41, the annular block 43 is fixedly arranged at the outer side of the spline rod 42 at the top of the cylindrical block 4a, the disc block 44 is arranged at the top of the annular block 43, the disc block 44 is in a petal shape, the second spline groove 45 is formed in the top of the disc block 44, the spline rod 42 penetrates through the second spline groove 45 and extends, an annular limiting plate 46 is fixedly mounted at the top of the cylindrical block 4a and positioned at the outer side of the disc block 44, a sliding hole is formed in the annular limiting plate 46 along the circumferential direction, a circular head plate 47 is arranged in the sliding hole in a sliding mode, one end, positioned at the outer side of the annular limiting plate 46, of the bottom of the circular head plate 47 is inclined, the thickness of the tail end of the bottom of the circular head plate 47 becomes thinner gradually from the center of the cylindrical block 4a to the edge, a compression spring 48 is arranged on the circular head plate 47, one end of the compression spring 48 is fixedly connected with the inner wall of the annular limiting plate 46, and the other end of the compression;
firstly, the separating device 5 is placed in a clamping groove on the cylindrical block 4a according to different cultured cell types, after the placing is completed, the wedge block 40 is also placed in the clamping groove, the wedge block 40 is enabled to extrude the separating device 5, the separating device 5 is enabled to be contacted with the inner wall of the ring shell 1, the spline rod 42 is enabled to move upwards, the spline rod 42 is enabled to be separated from the first spline groove 41, the spline rod 42 is rotated at the moment, the spline rod 42 is matched with the second spline groove 45, the spline rod 42 drives the disc block 44 to rotate, the disc block 44 extrudes the disc block 47, at the moment, under the action of the compression spring 48, a plurality of groups of disc blocks 47 are enabled to simultaneously open outwards, the bottom of the disc block 47 is enabled to be contacted with the top of the wedge block 40, the disc block 47 synchronously presses down the wedge block 40 and limits the wedge block 40, and finally the spline rod 42 is placed in the first spline groove 41, therefore, the separating device 5 is limited, the separating device 5 is prevented from shaking, different types of cells needing to be cultured are placed in the separating device 5 at the moment, when the separating device 5 needs to be taken out, the spline rod 42 moves upwards, and when the cells are separated from the first spline groove 41, the cells are reversed, the circular head plate 47 does not limit the wedge block 40 any more, the wedge block 40 is taken out firstly, and finally the separating device 5 is taken out;
the bottom of the disc block 44 is provided with an annular groove, and the top of the annular block 43 is positioned in the annular groove;
the annular groove is formed at the bottom of the disc block 44, so that the disc block 44 is supported by the annular block 43, and the rotation of the disc block 44 is not influenced;
a first pull ring is uniformly arranged on the outer side wall of the annular limiting plate 46 along the circumferential direction, a second pull ring is arranged at one end, close to the annular limiting plate 46, of the wedge block 40, and the first pull ring and the second pull ring are connected through an anti-lost rope 461;
through the anti-lost rope 461, the wedge-shaped block 40 is prevented from being lost due to the structural limitation of the wedge-shaped block 40, and the anti-lost rope is not easy to find.
The separating device 5 comprises a fan-shaped isolation plate 51, a fan-shaped groove 52, a rubber pad 53 and a clamping block 54, wherein a plurality of groups of fan-shaped isolation plates 51 are uniformly arranged on the top of the circular base 3 in the circular shell 1, the fan-shaped groove 52 is formed in the top of the fan-shaped isolation plate 51, the rubber pad 53 is fixedly arranged at one end of the fan-shaped isolation plate 51, which is positioned on the inner wall of the circular shell 1, the clamping block 54 is fixedly arranged at the other end of the fan-shaped isolation plate 51, and the clamping block 54 is positioned in the clamping groove;
firstly, different numbers of clamping blocks 54 are placed in clamping grooves according to different types of cultured cells, so that the clamping blocks 54 drive the fan-shaped isolation plate 51 to be contacted with the circular base 3, after the clamping blocks are placed, the rubber pads 53 on the fan-shaped isolation plate 51 are in close contact with the inner wall of the circular shell 1 according to the limiting device 4, the fan-shaped isolation plate 51 is prevented from shaking, the culture of the cells is influenced, and finally, the cells of different types to be cultured are placed in the fan-shaped grooves 52 on the fan-shaped isolation plate 51;
the front side and the rear side of the side wall of the circular shell 1 are symmetrically and fixedly provided with a v-shaped handle 12, a v-shaped handle 21274, the corners of the inner wall and the outer wall of the v-shaped handle 12 are arc-shaped; through the arranged v-shaped handle 12, the v-shaped handle 12 is convenient to take and is curved at the corner of the v-shaped handle 12, so that the appearance is attractive, and the potential safety hazard is avoided;
the inner side wall of the circular shell 1 is uniformly provided with a first arc-shaped groove along the circumferential direction; through the first arc-shaped grooves uniformly formed in the inner side wall of the circular shell 1, when the limiting device 4 enables the rubber pads 53 on the fan-shaped partition plates 51 to be in contact with the inner wall of the circular shell 1, the first arc-shaped grooves can increase friction force, so that the rubber pads 53 are attached to the inner side wall of the circular shell 1 more tightly;
one end of the clamping block 54 close to the side wall of the cylindrical block 4a is inclined, and the inclination angle is gradually increased from top to bottom;
with the inclined clamping block 54, when the wedge block 40 pushes the clamping block 54 so that the sector isolation plate 51 contacts the inner wall of the ring housing 1, the inclined surface of the wedge block 40 just matches with the inclined surface of the clamping block 54.
The cover body 2 comprises a circular cover 21, an annular hollow cylinder 22, a first-type \ 21274, a shaped block 23, a clamping groove 24, a displacement groove 25, an L-shaped clamping plate 26 and a telescopic spring rod 27, wherein the bottom of the circular cover 21 is fixedly provided with the annular hollow cylinder 22, the annular hollow cylinder 22 is matched with the circular shell 1, the inner side wall of the annular hollow cylinder 22 is provided with the first-type \ 21274, the shaped block 23 is provided with the outer side wall of the annular hollow cylinder 22 at the first-type 21274, the position of the shaped block 23 is provided with the clamping groove 24 and the first-type \ 21274, the vertical sections at the two ends of the shaped block 23 are provided with the displacement groove 25, two groups of L-shaped clamping plates 26 are arranged in the clamping groove 24, and the vertical sections of the two groups of L-;
after the limiting device 4 limits the separating device 5 according to different types of stem cells, different types of cells to be cultured are placed in the fan-shaped grooves 52 on the fan-shaped separating plate 51, two sets of L-shaped clamping plates 26 are pinched at the moment, the two sets of L-shaped clamping plates 26 are contracted under the action of the telescopic spring rods 27, the annular hollow cylinder 22 on the circular cover 21 is placed on the circular shell 1, and after the placement is finished, the L-shaped clamping plates 26 are loosened, so that the annular hollow cylinder 22 and the circular shell 1 are locked, the locking of the annular hollow cylinder 22 and the circular shell 1 caused by the conventional thread rotation is avoided, the stability is increased, and the circular shell 1 is prevented from rotating to damage the stem cells in the separating device 5;
the middle position of the top of the circular cover 21 is fixedly provided with a handle 211, the bottom of the handle 211 is provided with a first circular groove, the center position of the bottom of the circular cover 21 is provided with a second circular groove, and the second circular groove is communicated with the first circular groove;
the spline rod 42 is convenient to take and store through the installed handle 211 and the first round groove and the second round groove;
a first rectangular groove 11 is formed in the left side wall and the right side wall of the circular shell 1, and positioning grooves are formed in the front end face and the rear end face of the first rectangular groove 11;
firstly, two groups of L-shaped clamping plates 26 are pinched, a I-shaped 21274on the annular hollow cylinder 22 is slid into a I-shaped rectangular groove 11 on the circular shell 1, after the L-shaped clamping plates 26 are placed, the L-shaped clamping plates 26 are loosened, so that the horizontal sections of the two groups of L-shaped clamping plates 26 penetrate through the I-shaped 21274, and a displacement groove 25 on the L-shaped clamping plates 23 is clamped into a positioning groove, so that the annular hollow cylinder 22 and the circular shell 1 are quickly locked, and the time is saved while the stem cells in the separating device 5 are not influenced;
the concrete during operation:
firstly, different numbers of clamping blocks 54 are placed in clamping grooves according to different types of cultured cells, so that the clamping blocks 54 drive the fan-shaped isolation plate 51 to be contacted with the circular base 3, after the clamping blocks are placed, the rubber pads 53 on the fan-shaped isolation plate 51 are in close contact with the inner wall of the circular shell 1 according to the limiting device 4, the fan-shaped isolation plate 51 is prevented from shaking, and finally, different types of cells to be cultured are placed in the fan-shaped grooves 52 on the fan-shaped isolation plate 51;
the second step, hold two sets of L shape clamp plates 26 this moment, make two sets of L shape clamp plates 26 shrink under the effect of telescopic spring pole 27, place the annular hollow cylinder 22 on the circular lid 21 on the ring casing 1 this moment again, after placing, loosen L shape clamp plate 26, thereby make the horizontal segment of two sets of L shape clamp plates 26 run through one \ 21274, displacement groove 25 joint on the shape piece 23 is to the constant head tank in, make annular hollow cylinder 22 and ring casing 1 lock fast, avoided traditional screw rotation to make the locking of annular hollow cylinder 22 and ring casing 1, thereby increase stability.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (8)

1. A stem cell culture vessel, includes ring casing (1) and lid (2), its characterized in that: a circular base (3) is fixedly installed at the bottom of the circular shell (1), a limiting device (4) is arranged in the circular shell (1) at the center of the top of the circular base (3), separating devices (5) are uniformly arranged in the circular shell (1) at the top of the circular base (3), and a cover body (2) is arranged above the circular shell (1);
the limiting device (4) comprises a cylindrical block (4a), a wedge block (40), a first spline groove (41), a spline rod (42), an annular block (43), a disc block (44), a second spline groove (45), an annular limiting plate (46), a round head plate (47) and a compression spring (48), wherein the cylindrical block (4a) is fixedly installed at the top of the circular base (3), clamping grooves are uniformly formed in the top of the cylindrical block (4a) along the circumferential direction, the wedge block (40) is arranged in the clamping grooves, the inclination angle of the wedge block (40) is gradually reduced from top to bottom, the first spline groove (41) is formed in the center position of the top of the cylindrical block (4a), the spline rod (42) is arranged in the first spline groove (41), the annular block (43) is fixedly installed on the outer side of the spline rod (42) at the top of the cylindrical block (4a), and the annular disc block (44) is arranged at the top of the cylindrical block (43), the disc block (44) is petal-shaped, a second spline groove (45) is formed in the top of the disc block (44), the spline rod (42) penetrates through the second spline groove (45) to extend upwards, an annular limiting plate (46) is fixedly mounted on the outer side, located on the disc block (44), of the top of the cylindrical block (4a), a sliding hole is formed in the annular limiting plate (46) along the circumferential direction, a disc plate (47) is arranged in the sliding hole in a sliding mode, one end, located on the outer side of the annular limiting plate (46), of the bottom of the round head plate (47) is inclined, the thickness of the tail end of the bottom of the disc plate (47) is gradually reduced from the center of the cylindrical block (4a) to the edge, a compression spring (48) is arranged on the disc plate (47), one end of the compression spring (48) is fixedly connected with the inner wall of the annular limiting plate (46), and the other end of the compression spring (;
the separating device (5) comprises a fan-shaped isolation plate (51), fan-shaped grooves (52), rubber pads (53) and clamping blocks (54), wherein a plurality of groups of fan-shaped isolation plates (51) are uniformly arranged on the top of the circular base (3) in the circular ring shell (1), the fan-shaped grooves (52) are formed in the top of the fan-shaped isolation plates (51), the rubber pads (53) are fixedly arranged at one ends of the fan-shaped isolation plates (51) on the inner wall of the circular ring shell (1), the clamping blocks (54) are fixedly arranged at the other ends of the fan-shaped isolation plates (51), and the clamping blocks (54) are arranged in the clamping grooves;
the cover body (2) comprises a circular cover (21), an annular hollow cylinder (22), a Contraband-shaped block (23), a clamping groove (24), a displacement groove (25), an L-shaped clamping plate (26) and a telescopic spring rod (27), the bottom of the circular cover (21) is fixedly provided with the annular hollow cylinder (22), the annular hollow cylinder (22) is matched with the circular ring shell (1), the inner side wall of the annular hollow cylinder (22) is provided with a v-21274, the shaped block (23), the outer side wall of the annular hollow cylinder (22) is located at a v-21274, the position of the shaped block (23) is provided with the clamping groove (24), and a v-21274, the vertical sections at two ends of the shaped block (23) are provided with the displacement groove (25), two sets of L-shaped clamping plates (26) are arranged in the clamping groove (24), and the vertical sections of the two sets of L-shaped clamping plates (26) are connected through the telescopic spring rod (27).
2. A stem cell culture vessel according to claim 1, wherein: a rectangular groove (11) is formed in the left side wall and the right side wall of the circular shell (1), and positioning grooves are formed in the front end face and the rear end face of the rectangular groove (11).
3. A stem cell culture vessel according to claim 1, wherein: the inner side wall of the circular ring shell (1) is uniformly provided with a first arc-shaped groove along the circumferential direction.
4. A stem cell culture vessel according to claim 1, wherein: an annular groove is formed in the bottom of the disc block (44), and the top of the annular block (43) is located in the annular groove.
5. A stem cell culture vessel according to claim 1, wherein: the front side and the rear side of the side wall of the circular shell (1) are symmetrically and fixedly provided with a v-shaped handle (12) and a v-shaped handle (12), wherein corners of the inner wall and the outer wall of the v-shaped handle (12) are arc-shaped.
6. A stem cell culture vessel according to claim 1, wherein: the handle (211) is fixedly installed at the middle position of the top of the circular cover (21), a circular groove is formed in the bottom of the handle (211), a circular groove is formed in the center of the bottom of the circular cover (21), and the circular groove is communicated with the circular groove.
7. A stem cell culture vessel according to claim 1, wherein: the outer side wall of ring-shaped limiting plate (46) evenly install a pull ring along circumference, and wedge (40) go up the one end that is close to ring-shaped limiting plate (46) and install No. two pull rings, a pull ring and No. two pull rings are connected through anti-lost rope (461).
8. A stem cell culture vessel according to claim 1, wherein: one end of the clamping block (54) close to the side wall of the cylindrical block (4a) is inclined.
CN202110556241.4A 2021-05-21 2021-05-21 Stem cell culture vessel Withdrawn CN113106024A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110556241.4A CN113106024A (en) 2021-05-21 2021-05-21 Stem cell culture vessel

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110556241.4A CN113106024A (en) 2021-05-21 2021-05-21 Stem cell culture vessel

Publications (1)

Publication Number Publication Date
CN113106024A true CN113106024A (en) 2021-07-13

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113478916A (en) * 2021-07-28 2021-10-08 鲍龙英 Non-woven cloth bag surface image-text color printing method
CN117327555A (en) * 2023-10-07 2024-01-02 康润景星(苏州)生物科技有限公司 Strain fermentation process beneficial to improvement of strain density

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113478916A (en) * 2021-07-28 2021-10-08 鲍龙英 Non-woven cloth bag surface image-text color printing method
CN117327555A (en) * 2023-10-07 2024-01-02 康润景星(苏州)生物科技有限公司 Strain fermentation process beneficial to improvement of strain density
CN117327555B (en) * 2023-10-07 2024-02-27 康润景星(苏州)生物科技有限公司 Strain fermentation process beneficial to improvement of strain density

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