CN113084191A - Preparation method of silver-coated gold nanorod - Google Patents
Preparation method of silver-coated gold nanorod Download PDFInfo
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 88
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 229910052709 silver Inorganic materials 0.000 title claims abstract description 46
- 239000004332 silver Substances 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 110
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 40
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims abstract description 37
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 20
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 19
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 239000012279 sodium borohydride Substances 0.000 claims abstract description 12
- 229910000033 sodium borohydride Inorganic materials 0.000 claims abstract description 12
- 101710134784 Agnoprotein Proteins 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims abstract description 10
- 239000002244 precipitate Substances 0.000 claims abstract description 9
- 239000011248 coating agent Substances 0.000 claims abstract description 8
- 238000000576 coating method Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 4
- 239000008363 phosphate buffer Substances 0.000 claims abstract description 4
- 239000011780 sodium chloride Substances 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 20
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(I) nitrate Inorganic materials [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 20
- 229910004042 HAuCl4 Inorganic materials 0.000 claims description 10
- 238000007792 addition Methods 0.000 claims description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 3
- 235000000069 L-ascorbic acid Nutrition 0.000 claims description 3
- 239000002211 L-ascorbic acid Substances 0.000 claims description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 230000009467 reduction Effects 0.000 claims description 3
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 238000003260 vortexing Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 239000002073 nanorod Substances 0.000 claims 1
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 description 10
- 206010028980 Neoplasm Diseases 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000003917 TEM image Methods 0.000 description 4
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- 201000011510 cancer Diseases 0.000 description 2
- VYXSBFYARXAAKO-WTKGSRSZSA-N chembl402140 Chemical compound Cl.C1=2C=C(C)C(NCC)=CC=2OC2=C\C(=N/CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-WTKGSRSZSA-N 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
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- 239000012620 biological material Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 229960000800 cetrimonium bromide Drugs 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 229940124447 delivery agent Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 238000012546 transfer Methods 0.000 description 1
- 238000004627 transmission electron microscopy Methods 0.000 description 1
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- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/07—Metallic powder characterised by particles having a nanoscale microstructure
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- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/17—Metallic particles coated with metal
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- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- B22F2301/00—Metallic composition of the powder or its coating
- B22F2301/25—Noble metals, i.e. Ag Au, Ir, Os, Pd, Pt, Rh, Ru
- B22F2301/255—Silver or gold
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Abstract
The invention discloses a preparation method of a silver-coated gold nanorod, which comprises the following steps: preparation of NaBH4Dissolving and reacting said NaBH4The solution was inserted into ice for at least 10 minutes; adding a CTAB solution of hexadecyl trimethyl ammonium bromide into a container, placing the container in a water bath at 30 ℃, and stirring the mixture at a speed of not higher than 600 rpm; adding HAuCl into the CTAB solution4Solution, then adding said NaBH under vigorous stirring at not less than 1000rpm4Obtaining a brown seed solution; and after 5 minutes, standing the seed solution in a water bath for 1 hour; stirring at 30 ℃ of not higher than 600rpmAdding AgNO into CTAB solution3Aqueous solution, then HAuCl is added4The solution, ascorbic acid solution and seed solution to obtain colorless mixed solution; stirring the colorless mixed solution for 1 minute, and then standing in the dark for 1 hour; transferring the mixed solution after standing to an Eppendorf tube, and centrifuging to remove supernatant to obtain solution precipitate; resuspending the solution precipitate in phosphate buffer saline to obtain gold nanorods; and forming a silver coating on the surface of the gold nanorod.
Description
Technical Field
The invention relates to the technical field of preparation of nano biological materials. Specifically, the invention relates to a preparation method of a silver-coated gold nanorod.
Background
The excellent performance of Gold Nanorods (GNRs) on Local Surface Plasmon Resonance (LSPR) and Surface Enhanced Raman Scattering (SERS) characteristics makes them have great potential in the fields of cancer imaging, biological and optical sensing, chemical catalysis, etc., and has been a research hotspot of scientists in the past decades.
For example, Gold Nanorods (GNRs) have a small volume but a large surface area, and thus can be used to load drugs and enhance the stability and solubility of drugs, and GNRs can deliver unstable drugs to areas of the body that would otherwise not be accessible as effective drug delivery agents. While GNRs have better absorption and scattering capabilities compared to other molecular species, so they can also be used for bio-imaging and for the production of new biomedical sensors and contrast agents. In addition, the GNR has the capacity of converting absorbed light into heat in a non-radiation process, so that the gold nanorods are enriched in the tumor by a certain method, the tumor is locally heated to kill tumor tissues under the irradiation of laser with specific wavelength, and surrounding normal tissues are not affected by the gold nanorods, so that the gold nanorods can be used for photo-thermal treatment of cancer.
In the mainstream gold nanorod synthesis process at present, Cetyl Trimethyl Ammonium Bromide (CTAB) is required to be used, so that the formed gold nanorod solution contains a large amount of CTAB. The CTAB layer greatly limits the application in the biology of gold nanorods due to its strong cytotoxicity and high bad ability of cell membrane.
The prior art generally removes CTAB on the surface of the gold nanorod by multiple washing. The washing method can remove part of CTAB on the surface of the gold nanorod, but on the other hand, other molecules or groups can be introduced on the surface of the gold nanorod. Therefore, a new method is needed in the art to solve the problem of CTAB toxicity on the surface of gold nanorods.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a preparation method of a silver-coated gold nanorod, which comprises the following steps:
preparation of NaBH4Dissolving and reacting said NaBH4The solution was inserted into ice for at least 10 minutes;
adding a CTAB solution of hexadecyl trimethyl ammonium bromide into a container, placing the container in a water bath at 30 ℃, and stirring the mixture at a speed of not higher than 600 rpm;
adding HAuCl into the CTAB solution4Solution, then adding said NaBH under vigorous stirring at not less than 1000rpm4Obtaining a brown seed solution; and
after 5 minutes, the seed solution was allowed to stand in a water bath for 1 hour;
adding AgNO into CTAB solution under stirring at 30 deg.C not higher than 600rpm3Aqueous solution, then HAuCl is added4The solution, ascorbic acid solution and seed solution to obtain colorless mixed solution;
stirring the colorless mixed solution for 1 minute, and then standing in the dark for 1 hour;
transferring the mixed solution after standing to an Eppendorf tube, and centrifuging to remove supernatant to obtain solution precipitate;
resuspending the solution precipitate in phosphate buffer saline to obtain gold nanorods; and
and forming a silver coating on the surface of the gold nanorod.
In one embodiment of the invention, the NaBH4The concentration of the solution is 0.01Mol/L, the concentration of the CTAB solution is 0.1Mol/L, and the HAuCl4The concentration of the solution was 0.01 Mol/L.
In one embodiment of the invention, the seed solution comprises 9.75ml CTAB solution, 0.25ml HAuCl4Solution and 0.6ml NaBH4And (3) solution.
In one embodiment of the invention, the AgNO3The concentration of the aqueous solution was 0.01Mol/L and the concentration of the ascorbic acid was 0.1 Mol/L.
In one embodiment of the invention, the gold nanorods comprise 130 μ l of AgNO3Aqueous solution, 9.5ml CTAB solution, 0.5ml HAuCl4Solution, 55. mu.l ascorbic acid, and 12. mu.l seed solution.
In one embodiment of the invention, the centrifugation speed is 8500rpm and lasts 15 minutes.
In one embodiment of the invention, the forming of the silver coating on the surface of the gold nanorod comprises adding silver nitrate and ascorbic acid solutions with different concentrations into the gold nanorod solution, then adding ammonia water, and mixing uniformly until the color does not change any more, so that the surface of the gold nanorod is coated with the silver layer.
In one embodiment of the invention, the transfer is to a 2mL centrifuge tube. To 0.5mL of the prepared gold nanorod solution was added 7.5. mu.L of 0.1M AgNO3And 0.1M ascorbic acid, followed by 3. mu.LNH4OH, AgNO reduction by ascorbic acid3The mixture was blackened, and the solution was centrifuged at 4000rpm for 10 minutes at 4 ℃ and then redispersed in ultrapure water.
In one embodiment of the invention, 7.5. mu.L of 0.1M AgNO was added3And 0.1M ascorbic acid can be added in multiple portions, with 10s vortexing after each addition.
The embodiment of the invention provides a method for synthesizing silver-coated gold nanorods. The gold nanorod coated with silver has stronger SERS performance. The surface of the formed silver-coated gold nanorod is free of surfactant, free of biotoxicity and good in biocompatibility. The synthesis method of the silver-coated gold nanorod provided by the invention has the advantages of simplicity in operation, rapidness, strong repeatability, low cost and the like.
Drawings
To further clarify the above and other advantages and features of embodiments of the present invention, a more particular description of embodiments of the invention will be rendered by reference to the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. In the drawings, the same or corresponding parts will be denoted by the same or similar reference numerals for clarity.
Fig. 1 illustrates a method for preparing silver-coated gold nanorods according to one embodiment of the present invention.
FIG. 2 shows a TEM image of a gold nanorod formed by an embodiment of the present invention.
Fig. 3 shows a transmission electron microscope TEM image of silver-coated gold nanorods formed by an embodiment of the present invention.
Fig. 4 shows a Surface Enhanced Raman Spectrum (SERS) of a 1 μ M solution into which gold nanorods formed by an embodiment of the present invention were dropped on a glass slide.
Fig. 5 shows a Surface Enhanced Raman Spectrum (SERS) of a 1 μ M solution into which silver-coated gold nanorods formed by an example of the present invention were dropped on a glass slide.
Detailed Description
In the following description, the invention is described with reference to various embodiments. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of the specific details, or with other alternative and/or additional methods, materials, or components. In other instances, well-known structures, materials, or operations are not shown or described in detail to avoid obscuring aspects of embodiments of the invention. Similarly, for purposes of explanation, specific numbers, materials and configurations are set forth in order to provide a thorough understanding of the embodiments of the invention. However, the invention may be practiced without specific details. Further, it should be understood that the embodiments shown in the figures are illustrative representations and are not necessarily drawn to scale.
Reference in the specification to "one embodiment" or "the embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment.
The embodiment of the invention discloses a method for synthesizing silver-coated gold nanorods. The gold nanorod particles solve the problem of CTAB cytotoxicity on the surfaces of the gold nanorods through silver coating, so that the gold nanorods have better biocompatibility and can maintain the stability of the gold nanorods, and meanwhile, the gold nanorods have stronger SERS (surface enhanced Raman scattering) characteristics due to the silver coating, so that the silver-coated gold nanorods can obtain a larger space in the application of biomedicine, such as biosensors, biomedical imaging, medical rapid detection and the like.
Fig. 1 illustrates a method for preparing silver-coated gold nanorods according to one embodiment of the present invention.
As shown in fig. 1, a seed solution is first prepared, comprising:
first, at step 1011, NaBH is prepared4And (3) solution. Preparation of NaBH at defined concentrations4Dissolving and reacting said NaBH4The solution was inserted into ice for at least 10 minutes; in one embodiment of the invention, the NaBH4The concentration of the solution is 0.01 Mol/L;
next, in step 1012, a CTAB solution is prepared. The CTAB solution is cetyltrimethylammonium bromide (Cetrimonium bromide), and is prepared by: adding a certain amount of CTAB solution of a protective agent with a specified concentration into a container, wherein the volume of the container is not less than 20mL, and the container can be a glass vial, a beaker or any other container convenient for stirring, and placing the container in a water bath with the temperature of 30 ℃ to slowly stir at the speed of not more than 600 rpm; in one embodiment of the invention, the concentration of CTAB solution is 0.1Mol/L, in another embodiment of the invention, CTAB solution is prepared in 9.75 ml;
next, at step 1013, a seed solution is synthesized. Adding HAuCl into the CTAB solution4The solution is then added with vigorous stirring at not less than 1000rpm to the NaBH prepared in step 10114Obtaining a seed solution, wherein the color of the solution is changed from bright yellow to dark brown in the process; in one embodiment of the invention, the HAuCl4The solution concentration was 0.01Mol/L, and in yet another embodiment of the invention, HAuCl was used40.25ml of solution and NaBH40.6ml of solution; and
finally, at step 1014, rest. After the solution prepared in step 1013 became tan for 5 minutes, the seed solution was allowed to stand in a water bath for 1 hour; and
synthesizing gold nanorods comprising:
first, at step 1021, the solution is mixed. AgNO is slowly stirred at a speed of not higher than 600rpm at a temperature of 30 DEG C3The aqueous solution was added to CTAB solution, followed by HAuCl4The solution, the ascorbic acid solution and the seed solution are added, and the color of the solution is changed from fresh yellow to colorless; in one embodiment of the invention, the AgNO3The concentration of the aqueous solution is 0.01Mol/L, the concentration of the CTAB solution is 0.1Mol/L, and the HAuCl4The concentration of the solution was 0.01Mol/L and the concentration of the ascorbic acid was 0.1Mol/L, and in yet another embodiment of the invention, the respective solutions were used in the following amounts: 30 μ l of AgNO3Aqueous solution, 9.5ml CTAB solution, 0.5ml HAuCl4Solution, 55. mu.l ascorbic acid, and 12. mu.l seed solution;
next, at step 1022, the solution is stirred. Stirring the colorless mixed solution obtained in the step 1021 for 1 minute, standing for 1 hour in the dark, and changing the color to red in about 15 minutes, which indicates that the synthesis of the gold nanorods is successful;
next, at step 1023, a precipitate is obtained. Transferring the mixed solution into an Eppendorf tube, centrifuging, and removing a supernatant to obtain a solution precipitate; in one embodiment of the invention, the centrifugation speed is 8500rpm for 15 minutes; and
finally, at step 1024, gold nanorods are obtained and stored. Resuspending the solution precipitate in phosphate buffer saline to obtain gold nanorods, and storing the gold nanorods at room temperature; in one embodiment of the invention, the solution pellet is resuspended in 10ml of phosphate buffered saline at pH 7.4;
next, in step 1031, a silver coating is formed on the surface of the gold nanorods. Specifically, silver nitrate and ascorbic acid solutions with different concentrations are added into a gold nanorod solution, then ammonia water is added, and the mixture is uniformly mixed until the color does not change any more, so that the surface of a gold nanorod is coated with a silver layer, and the silver-coated gold nanorod solution is obtained.
For example, in one example, 0.5mL of a previously prepared gold nanorod solution is transferred to a 2mL centrifuge tube. Add 7.5. mu.L of 0.1M AgNO to the tube3And 0.1M Ascorbic Acid (AA). Multiple additions were made, with 10s vortexing after each addition. Then 3. mu.L NH was added4OH, AgNO reduction by ascorbic acid AA3The mixture turns black. The solution will stabilize in about 5 minutes. Finally, the solution was centrifuged at 4000rpm for 10 minutes at 4 ℃ and then redispersed in ultrapure water.
Next, the silver coating layer of the gold nanorods was studied by using ultraviolet-visible light (UV-Vis) absorption spectroscopy and TEM transmission electron microscopy.
FIG. 2 shows a TEM image of a gold nanorod formed by an embodiment of the present invention.
Fig. 3 shows a transmission electron microscope TEM image of silver-coated gold nanorods formed by an embodiment of the present invention. As shown in fig. 3, the gold nanorods were completely covered with silver, and the particle diameter became larger after the surface of the gold nanorods was covered with silver.
Rhodamine 6G (R6G) was used as a model analyte to test the surface raman enhancement performance of gold nanorods and silver-coated gold nanorods. Fig. 4 shows a Surface Enhanced Raman Spectrum (SERS) of a 1 μ M solution into which gold nanorods formed by an embodiment of the present invention were dropped on a glass slide. Fig. 5 shows a Surface Enhanced Raman Spectrum (SERS) of a 1 μ M solution into which silver-coated gold nanorods formed by an example of the present invention were dropped on a glass slide. As can be seen from fig. 4 and 5, the signal intensity from the silver-coated gold nanorods is an order of magnitude higher than that of the gold nanorods.
The embodiment of the invention provides a method for synthesizing silver-coated gold nanorods. The gold nanorod coated with silver has stronger SERS performance. The surface of the formed silver-coated gold nanorod is free of surfactant, free of biotoxicity and good in biocompatibility. The synthesis method of the silver-coated gold nanorod provided by the invention has the advantages of simplicity in operation, rapidness, strong repeatability, low cost and the like.
While various embodiments of the present invention have been described above, it should be understood that they have been presented by way of example only, and not limitation. It will be apparent to persons skilled in the relevant art that various combinations, modifications, and changes can be made thereto without departing from the spirit and scope of the invention. Thus, the breadth and scope of the present invention disclosed herein should not be limited by any of the above-described exemplary embodiments, but should be defined only in accordance with the following claims and their equivalents.
Claims (9)
1. A preparation method of silver-coated gold nanorods comprises the following steps:
preparation of NaBH4Dissolving and reacting said NaBH4The solution was inserted into ice for at least 10 minutes;
adding a CTAB solution of hexadecyl trimethyl ammonium bromide into a container, placing the container in a water bath at 30 ℃, and stirring the mixture at a speed of not higher than 600 rpm;
adding HAuCl into the CTAB solution4Solution, then adding said NaBH under vigorous stirring at not less than 1000rpm4Obtaining a brown seed solution; and
after 5 minutes, the seed solution was allowed to stand in a water bath for 1 hour;
adding AgNO into CTAB solution under stirring at 30 deg.C not higher than 600rpm3Aqueous solution, then HAuCl is added4The solution, ascorbic acid solution and seed solution to obtain colorless mixed solution;
stirring the colorless mixed solution for 1 minute, and then standing in the dark for 1 hour;
transferring the mixed solution after standing to an Eppendorf tube, and centrifuging to remove supernatant to obtain solution precipitate;
resuspending the solution precipitate in phosphate buffer saline to obtain gold nanorods; and
and forming a silver coating on the surface of the gold nanorod.
2. The method of claim 1, wherein the gold nanorods are coated with silverThe preparation method is characterized in that the NaBH is prepared by4The concentration of the solution is 0.01Mol/L, the concentration of the CTAB solution is 0.1Mol/L, and the HAuCl4The concentration of the solution was 0.01 Mol/L.
3. The method of preparing silver-coated gold nanorods according to claim 2, wherein the seed solution comprises 9.75ml CTAB solution, 0.25ml HAuCl4Solution and 0.6ml NaBH4And (3) solution.
4. The method of preparing silver-coated gold nanorods according to claim 1, wherein the AgNO is3The concentration of the aqueous solution was 0.01Mol/L and the concentration of the ascorbic acid was 0.1 Mol/L.
5. The method of preparing silver-coated gold nanorods according to claim 4, wherein the gold nanorods comprise 130 μ l of AgNO3Aqueous solution, 9.5ml CTAB solution, 0.5ml HAuCl4Solution, 55. mu.l ascorbic acid, and 12. mu.l seed solution.
6. The method of preparing silver-coated gold nanorods according to claim 1, wherein the centrifugation rotation speed is 8500rpm and lasts 15 minutes.
7. The method of preparing silver-coated gold nanorods according to claim 1, wherein forming a silver coating on the surface of the gold nanorods comprises adding silver nitrate and ascorbic acid solutions of different concentrations into the gold nanorod solution, then adding ammonia water, and mixing uniformly until the color does not change any more, so that the surface of the gold nanorods is coated with the silver layer.
8. The method of preparing silver-coated gold nanorods according to claim 1, wherein the nanorods are transferred into a 2mL centrifuge tube. To 0.5mL of the prepared gold nanorod solution was added 7.5. mu.L of 0.1M AgNO3And 0.1M ascorbic acid, then 3. mu.L NH was added4OH, AgNO reduction by ascorbic acid3Mixtures ofThe solution was blackened, centrifuged at 4000rpm for 10 minutes at 4 ℃ and then redispersed in ultrapure water.
9. The method of preparing silver-coated gold nanorods according to claim 1, wherein 7.5 μ L of 0.1M AgNO is added3And 0.1M ascorbic acid can be added in multiple portions, with 10s vortexing after each addition.
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