CN113075345A - Method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting high performance liquid chromatography - Google Patents
Method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting high performance liquid chromatography Download PDFInfo
- Publication number
- CN113075345A CN113075345A CN202110345955.0A CN202110345955A CN113075345A CN 113075345 A CN113075345 A CN 113075345A CN 202110345955 A CN202110345955 A CN 202110345955A CN 113075345 A CN113075345 A CN 113075345A
- Authority
- CN
- China
- Prior art keywords
- compost
- performance liquid
- liquid chromatography
- high performance
- peak
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting a high performance liquid chromatography, which comprises the steps of extracting organic matters in a sample of biomass raw material aerobic high-temperature compost at any sampling time by using an organic extracting agent to obtain an extracting solution, analyzing organic matter components in the extracting solution by using the high performance liquid chromatography to obtain an HPLC spectrogram of the organic matters in the compost, and determining the content proportion of the organic matters with different polarities according to the area percentage of peak areas at different peak emergence times in the spectrogram; and (3) analyzing and comparing HPLC spectrograms of organic matters in the compost at any sampling time, and determining that the tested compost is decomposed stably when the content proportion of the organic matters with different polarities reaches a certain value. Compared with the conventional method for judging whether the compost is rotten and stable by monitoring the temperature, the seed germination index and the like in the prior art, the method provided by the invention is a more accurate and convenient method for judging whether the compost is rotten and stable in nature.
Description
Technical Field
The invention relates to a method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting a high performance liquid chromatography, belonging to the technical field of livestock and poultry manure resource utilization.
Background
In recent years, with the increasing national economy and the continuous improvement of the living standard of residents, the market demand of livestock and poultry is increased, which promotes the development of livestock and poultry breeding to a certain extent. The livestock and poultry breeding mode is gradually changed from decentralized feeding to regionalization, mechanization and intensification, and the expansion speed of the livestock and poultry breeding mode is gradually increased year by year. Meanwhile, although the livestock and poultry breeding method meets the market and economic requirements, the amount of the livestock and poultry excrement discharged in the process is very large, and the excrement has unpleasant pungent odor. Relevant investigations have shown that the total discharge of livestock and poultry manure in the country is as high as 19.00 million tons in 2010, of which about 2.27 million tons is a serious pollution source. If the fertilizer is applied to farmlands, the average manure pollution amount per hectare of farmlands can reach 1.86 tons. If no strong policy is provided for pollution control of livestock and poultry manure, the total pollution amount is further expanded and is predicted to increase to 2.98 hundred million tons in 2020. The national first pollution source census bulletin shows that the chemical oxygen demand discharged by livestock and poultry breeding in 2010 reaches 1268.26 ten thousand tons, and accounts for 96 percent of the total amount discharged by agricultural sources; the total nitrogen and phosphorus emissions were 102.48 and 16.04 million tons, accounting for 38 and 56% of the total agricultural source emissions, respectively. Currently, the relevant data of the Ministry of agriculture shows that the livestock and poultry waste production amount is about 38 hundred million tons every year in China. A large amount of livestock and poultry manure is discharged into the environment without professional environment-friendly treatment, so that heavy burden is brought to ecological environment and social environment and even serious threat is brought to human health.
Relevant researches show that compost treatment is a convenient treatment mode for reducing, harmlessly treating and recycling agricultural wastes. Under the condition of manual control, animal and plant remains and excrement containing fertilizer components are added with soil and mineral substances and then mixed and stacked, and under the conditions of high temperature and high humidity, organic macromolecular substances are decomposed into micromolecular substances which can be absorbed and utilized by crops through the action of microorganisms, so that the process is a mineralization, putrefaction and harmless process of organic substances. The decomposed compost can not only improve the yield and the quality of crops, but also increase the organic matter content of soil and improve the physical and chemical properties of the soil. However, after the non-decomposed compost is applied to soil, a large amount of oxygen in the root soil is consumed due to the strong decomposition of organic matters, and toxic substances such as organic acids and the like are generated to inhibit the growth of crops. At present, much research is carried out on the maturity at home and abroad, and the temperature, the germination index of seeds, the pH value, the C/N ratio and the like are common maturity judgment indexes. However, most studies show that most of the indicators can only be used as reference indicators for compost stability and maturity rather than as independent indicators. Therefore, whether the compost sample is decomposed stably or not can be judged more accurately and conveniently, and the method has great significance for practical production application and scientific research.
Disclosure of Invention
In order to solve the problems of more accurately and conveniently judging whether compost is decomposed stably and saving the cost of practical production application and scientific research, the invention provides a method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting a high performance liquid chromatography.
The method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography is characterized by comprising the following steps of: extracting organic matters in the aerobic high-temperature compost of the biomass raw material by using an organic extractant to obtain an extracting solution; then analyzing the organic matter components in the extracting solution by high performance liquid chromatography to obtain HPLC spectrograms of the organic matter in the samples of the aerobic high-temperature compost at different sampling times, judging the polarity performance of the organic matter in the compost material according to the peak-out time in the spectrograms, and determining the content proportion of the organic matter with different polarities according to the area percentage of peak areas at different peak-out times in the spectrograms; analyzing and comparing HPLC spectrograms of organic matters in samples at different sampling times, and judging whether the determined compost is mature and stable or not according to the change rule of the content proportion of the organic matters with different polarities; and when the content proportion of the organic matters with different polarities reaches a certain value, determining that the measured compost is decomposed stably.
The method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography is characterized in that in the step one, the biomass raw materials are livestock and poultry manure, plant straws, plant residues, agricultural product processing waste, biological fermentation filter residues, kitchen waste, rural perishable waste and the like.
The method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography is characterized in that the organic extracting agent is n-butyl alcohol, and the process of extracting and extracting organic matters in a sampling sample comprises the following steps: adding n-butanol into the sample, ultrasonic extracting for 60-120 min, vacuum filtering, filtering the filtrate with 0.45 μm organic filter membrane, concentrating, re-dissolving the concentrate with mobile phase of high performance liquid chromatography, and analyzing with high performance liquid chromatography.
The method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography is characterized in that the use conditions of the high performance liquid chromatograph are as follows: the chromatographic column is a C18 column (5 μm, 4.6X 250 mm), and a gradient elution procedure is adopted, methanol and water are used as mobile phases, and the flow rate is kept at 0.8 mL/min; the column temperature is 25 ℃; the sample volume is 20 mu L; PDAD ultraviolet scanning, and setting 245 nm as a detection wavelength.
Further, the steps of using a gradient elution procedure are: the volume ratio of the methanol to the water is =1:9 within 0-20 min of retention time; the volume ratio of the methanol to the water is =3:7 within the retention time of 20-40 min; and the volume ratio of the methanol to the water is =9:1 within the retention time of 40-160 min.
The method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography is characterized in that the polarity types of organic matters in compost materials are divided into strong polarity, medium polarity, weak polarity and non-polarity according to the peak emergence time in the high performance liquid chromatogram, the peak emergence time is 0-20 min as a strong polarity matter peak, 21-40 min as a strong polarity matter peak, 41-60 min as a medium polarity matter peak, 61-80 min as a weak polarity matter peak and 81-160 min as a non-polarity matter peak.
The method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography is characterized in that the proportion of the peak area of the strong polar substance peak in the total peak area is less than 12%, and meanwhile, the proportion of the peak area of the medium polar substance peak in the total peak area is more than 70%, and the measured compost is determined to be decomposed stably.
Compared with the prior art, the invention has the following beneficial effects:
the essence of the composting process is the process of organic matter stabilization and humification. Along with the prolonging of the composting time, the glucide and the like are easy to degrade organic components, lignin and the like and gradually convert to macromolecular humus with high stability. The invention provides a method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting a high performance liquid chromatography, which can judge whether the aerobic high-temperature compost is decomposed stably according to the composition of organic substances in the compost. Compared with the conventional method for judging the composting time by monitoring the temperature, the seed germination index and the like in the prior art, the method provided by the invention is a more accurate and convenient method for judging whether the compost is stably composted or not in nature.
Drawings
FIG. 1 is a graph comparing the composting temperature trend with the composting time for the control group and the test group;
in fig. 1: Δ represents the control group results, o represents the test group results, and ● represents the daily average air temperature;
FIG. 2 is a graph comparing the germination percentage of plant seeds when the plant seeds are cultured with fertilizers of the control group and the test group at different composting times according to the present invention;
in fig. 2: Δ represents the control group results, and o represents the test group results;
FIG. 3 is a comparison graph of the proportion of organic substances with different polarities in fertilizers of a control group and a test group of the invention when the fertilizers at different composting times are subjected to component analysis;
in fig. 3: panel a shows the control results and panel b shows the test results.
Detailed Description
The present invention is further illustrated by the following examples, which should not be construed as limiting the scope of the invention.
Example 1:
1) compost raw materials to be tested:
fresh pig manure is taken from a certain scale pig farm in Hangzhou city, and the water content is close to 80 percent.
2) Regulating and controlling the water content of the compost raw materials:
control group: adding wood chips into fresh pig manure to enable the mass content of the wood chips in the final mixture to be 20%;
test groups: spreading fresh pig manure in a maggot groove padded with a base material, and then uniformly scattering a certain amount of fly maggot inoculant on the surface of the manure to allow the fly maggots to freely enter a manure pile for feeding and growing; wherein the housefly larva inoculant is a mixture of wheat bran (used for hatching housefly eggs) and housefly first-instar larvae (not more than 24 hours after hatching), each gram contains about 3000 housefly first-instar larvae, and the inoculation amount of the housefly larva inoculant is 0.75 percent of the total mass of fresh pig manure. And the residue (maggot manure) left after 8 d cultivation is carried out according to the cultivation process of DB 33/T2149-2018 standard is used as a compost raw material, and the moisture content of the maggot manure is about 65 percent after the maggot manure is subjected to biological conversion.
3) Composting treatment:
composting the fertilizers obtained from the control group or the test group in the step 2) respectively, wherein the process is as follows:
the method comprises the steps of stacking the fertilizer into a strip-shaped stack body with the length of 2.0 m, the width of 1.5 m and the height of 1.0 m, controlling the ventilation condition by adopting a manual stack turning measure, turning the stack once every four days during the temperature rise period of the stack body, turning the stack once every 2-3 days during the high-temperature period, immediately turning the stack when the temperature of the stack body exceeds 65 ℃ during the high-temperature period, and controlling the temperature of the high-temperature period to be not higher than 75 ℃. And turning the stack once every 7 days after the stack starts to cool. Samples were taken at compost 0d, 2d, 13d, 19d, 22d, 39d, 52d, 55d, 61d, 70d, 72d, 87d, 92d, respectively.
4) The determination method comprises the following steps:
ambient temperature: the temperature of the air around the stack was measured with a wall thermometer;
composting temperature: during the conversion of pig manure fly maggots, inserting a mercury thermometer into the fertilizer pile body to measure the temperature at a depth of 4 cm, and randomly measuring 3 points; during aerobic high-temperature composting, 3 mercury thermometers with a length of 50 cm were inserted daily into the surface of each heap to a depth of 30 cm.
Germination index testing process: adding 20 g of sample into 200 mL of distilled water, oscillating for 60 min, filtering supernatant by using slow filter paper, and obtaining filtrate, namely compost leaching liquor, wherein the filtrate is for later use; a piece of filter paper with the corresponding size is laid in a 9 cm culture dish, 15 pakchoi seeds are evenly placed in the filter paper, 5.0 mL of compost leaching liquor is absorbed in the culture dish by a pipette, and distilled water is used as a control test. Each treatment was repeated 3 times. Then placing in dark at (25 + -1) deg.C for 48 hr, and determining germination rate and root length according to formula
The germination index GI (%) was calculated.
Organic matter polarity change: and (3) analyzing the sample in the step 3), weighing 5 g (accurately recording to 0.0001 g) of sample of the compost material, placing the sample in a 250 ml conical flask, adding 30 ml of n-butyl alcohol as an extracting agent, performing ultrasonic leaching for 80 min, performing suction filtration by a Buchner funnel, filtering the filtrate by a 0.45-micron organic system filter membrane, concentrating the filtrate at 105 ℃, re-dissolving the concentrate by using a mobile phase of a high performance liquid chromatography, and analyzing by using a high performance liquid chromatograph. The conditions of the HPLC are as follows: the chromatographic column is a C18 column (5 μm, 4.6X 250 mm), and a gradient elution procedure is adopted, methanol and water are used as mobile phases, and the flow rate is kept at 0.8 mL/min; the volume ratio of the methanol to the water is =1:9 within 0-20 min of retention time; the volume ratio of the methanol to the water is =3:7 within the retention time of 20-40 min; the volume ratio of the methanol to the water is =9:1 within 40-160 min of retention time; the column temperature is 25 ℃; the amount of the sample was 20. mu.L. PDAD ultraviolet scanning, and setting 245 nm as a detection wavelength.
5) And (3) test results:
FIG. 1 is a graph comparing the composting temperature trend with the composting time for the control group and the test group.
As can be seen from figure 1, the temperature of the pig manure compost is raised from 26 ℃ to 51 ℃ in the temperature raising period of 0-17 days; the reactor temperature is in a small-amplitude fluctuation state within 18-24 days. After 25 days, the temperature of the pile gradually becomes stable and is kept at about 50 ℃. Then entering a cooling period at about 38 days of composting. According to the national standard GB 7959-87 for composting of Chinese excrement, when the composting temperature is kept above 50-55 ℃ for 5-7 days, the excrement can be subjected to harmless treatment. Therefore, the pig manure compost reaches the rotten requirement after 38 days.
As can be seen from figure 1, the whole compost fermentation process of maggot manure can be divided into four stages of maggot treatment period, high temperature period, medium temperature period and stabilization period. The temperature of the manure pile is slowly increased in the maggot treatment stage of 0-8 days, after the maggot manure is separated and aerobic high-temperature composting is carried out, the temperature of the pile body is rapidly increased, and the temperature is kept at 55-60 ℃ within 10-20 days, so that the compost sterilization requirements are completely met.
The study of scholars shows that when the germination index of the seeds exceeds 60 percent, the compost is decomposed. FIG. 2 shows the germination index trend of Chinese cabbage seeds. It can be seen that the seeds did not germinate neither for the first 6 days of fly maggot treatment nor for the first 20 days of pig manure composting, indicating the presence of phytotoxicity. The phytotoxicity of the two composts gradually decreased and the germination index gradually increased with time. Wherein, the germination index of the maggot manure compost is more than 60 percent about 20 days, and the germination index of the pig manure compost is more than 60 percent about 40 days, so as to reach a rotten stable state.
The essence of the composting process is the process of organic matter stabilization and humification. Along with the prolonging of the composting time, the glucide and the like are easy to degrade organic components, lignin and the like and gradually convert to macromolecular humus with high stability. According to the difference of the peak-off time in the high performance liquid chromatogram, the organic substances are divided into 5 classes of strong polarity (A), strong polarity (B), medium polarity (C), weak polarity (D) and non-polarity (E), wherein the peak-off time of A is 0-20 min, that of B is 21-40 min, that of C is 41-60 min, that of D is 61-80 min, and that of E is 81-160 min. The partition ratio (i.e., percentage) of the amount of each substance is equal to the proportion of the area of the substance in the total area of the substance. As shown in FIG. 3, the variation of the strongly polar and moderately polar substances with the composting time is large, and the strongly polar organic substances generally show a downward trend, and the moderately polar organic substances generally show an upward trend. Along with the prolonging of time, the proportion of the strong polarity organic matters and the proportion of the medium polarity organic matters in maggot manure on the 20 th day of composting and the proportion of the pig manure on the about 40 th day of composting both meet the requirement that the proportion of the strong polarity organic matters is less than 12 percent and the proportion of the medium polarity organic matters is more than 70 percent, which indicates that the composting treatment has reached a rotten and stable state. This conclusion was also validated against compost temperature and seed germination index analysis.
In the figures 1, 2 and 3, the composting time of the test group is divided into two stages, the first stage corresponds to 0-8 days of the maggot bioconversion process in the step 2) of the example 1, and the second stage corresponds to the composting process in the step 3) of the example 1.
The statements in this specification merely set forth a list of implementations of the inventive concept and the scope of the present invention should not be construed as limited to the particular forms set forth in the examples.
Claims (7)
1. A method for analyzing and determining whether aerobic high-temperature compost is decomposed stably by adopting a high performance liquid chromatography is characterized by comprising the following steps: extracting organic matters in the aerobic high-temperature compost of the biomass raw material by using an organic extractant to obtain an extracting solution; then analyzing the organic matter components in the extracting solution by high performance liquid chromatography to obtain HPLC spectrograms of the organic matter in the samples of the aerobic high-temperature compost at different sampling times, judging the polarity performance of the organic matter in the compost material according to the peak-out time in the spectrograms, and determining the content proportion of the organic matter with different polarities according to the area percentage of peak areas at different peak-out times in the spectrograms; analyzing and comparing HPLC spectrograms of organic matters in the compost at different sampling times, and judging whether the measured compost is mature and stable or not according to the change rule of the content proportion of the organic matters with different polarities; and when the content proportion of the organic matters with different polarities reaches a certain value, determining that the measured compost is stable in compost maturity.
2. The method for determining whether aerobic high-temperature compost is decomposed stably by high performance liquid chromatography analysis as claimed in claim 1, wherein the biomass raw material is at least one of livestock and poultry manure, plant straw, plant residue, agricultural product processing waste, biological fermentation residue, kitchen waste, rural perishable waste.
3. The method for determining whether aerobic high-temperature compost is decomposed stably by adopting high performance liquid chromatography analysis as claimed in claim 1, wherein the organic extractant is n-butanol, and the process of extracting and extracting the organic matters in the compost sample comprises the following steps: adding n-butanol into sample, ultrasonic extracting for 60-120 min, vacuum filtering, filtering the filtrate with 0.45 μm organic filter membrane, concentrating, re-dissolving the concentrate with mobile phase of high performance liquid chromatography, and analyzing with high performance liquid chromatography.
4. The method for determining whether aerobic high-temperature compost is decomposed stably by adopting the high performance liquid chromatography analysis as claimed in claim 1, wherein the high performance liquid chromatography is used under the following conditions: the chromatographic column is C18 column (5 μm, 4.6 × 250 mm), and gradient elution procedure is adopted, methanol and water are used as mobile phase, and the column temperature is 25 deg.C; the sample volume is 20 mu L; PDAD ultraviolet scanning, and setting 245 nm as a detection wavelength.
5. The method for determining whether aerobic high-temperature compost is decomposed stably by high performance liquid chromatography analysis as claimed in claim 4, wherein the step of using a gradient elution program comprises: the flow rate of the mobile phase was maintained at 0.8 mL/min; the volume ratio of the methanol to the water is =1:9 within 0-20 min of retention time; the volume ratio of the methanol to the water is =3:7 within the retention time of 20-40 min; and the volume ratio of the methanol to the water is =9:1 within the retention time of 40-160 min.
6. The method for determining whether aerobic high-temperature compost is decomposed stably by adopting high performance liquid chromatography analysis as claimed in claim 4, wherein the polarity types of organic matters in compost materials are divided into strong polarity, medium polarity, weak polarity and non-polarity according to the peak emergence time in the high performance liquid chromatogram, the peak emergence time is 0-20 min as a strong polarity material peak, 21-40 min as a strong polarity material peak, 41-60 min as a medium polarity material peak, 61-80 min as a weak polarity material peak, and 81-160 min as a non-polarity material peak.
7. The method for determining whether aerobic high-temperature compost is rotten stably by high performance liquid chromatography analysis according to claim 5, wherein the proportion of the peak area of the strong polar substance peak in the total peak area is less than 12%, and the proportion of the peak area of the medium polar substance peak in the total peak area is more than 70%, and the determined compost is determined to be rotten stably.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110345955.0A CN113075345B (en) | 2021-03-31 | 2021-03-31 | Method for analyzing and determining whether aerobic high-temperature compost is decomposed stably |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110345955.0A CN113075345B (en) | 2021-03-31 | 2021-03-31 | Method for analyzing and determining whether aerobic high-temperature compost is decomposed stably |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113075345A true CN113075345A (en) | 2021-07-06 |
| CN113075345B CN113075345B (en) | 2022-06-24 |
Family
ID=76614023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110345955.0A Active CN113075345B (en) | 2021-03-31 | 2021-03-31 | Method for analyzing and determining whether aerobic high-temperature compost is decomposed stably |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113075345B (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5583045A (en) * | 1995-01-30 | 1996-12-10 | Bedminster Bioconversion Corporation | Compost curing and odor control system |
| JP2003090839A (en) * | 2001-09-18 | 2003-03-28 | Toru Ueda | Endocrine disrupting substance group and method for evaluating biotic waste processed product using its activity as indicator |
| CN101097211A (en) * | 2006-06-28 | 2008-01-02 | 宝山钢铁股份有限公司 | Method for detecting molecular weight of strong-polarity organic matters in coking wastewater biochemical treatment discharge liquid |
| CN101774828A (en) * | 2010-01-28 | 2010-07-14 | 浙江省农业科学院 | Composting method for biologically dehydrating livestock manure |
| WO2014143741A1 (en) * | 2013-03-15 | 2014-09-18 | Gaia Institute | A method for composting organic waste |
| CN104355711A (en) * | 2014-11-03 | 2015-02-18 | 同济大学 | Aerobic composting method for animal dung with humus |
| CN104829075A (en) * | 2015-04-28 | 2015-08-12 | 上海元钧环保科技有限公司 | Fermentation rotten degree judging method based on humus content |
| JP2017160100A (en) * | 2016-03-11 | 2017-09-14 | トヨタ自動車株式会社 | Method of producing compost in which germination inhibitor is reduced |
| CN107236685A (en) * | 2017-05-22 | 2017-10-10 | 中国科学院成都生物研究所 | A kind of method that utilization sludge produces γ polyglutamic acid organic fertilizers |
| CN108530648A (en) * | 2018-03-13 | 2018-09-14 | 天津大学 | A kind of extraction and purification method of sludge composting fulvic acid |
| CN110117565A (en) * | 2019-05-28 | 2019-08-13 | 青岛力力惠生物科技股份有限公司 | A kind of biology decomposing agent and its application in compost |
| CN110627543A (en) * | 2019-10-30 | 2019-12-31 | 江苏省农业科学院 | Method for promoting compost maturity of livestock and poultry manure through microbial pretreatment |
| CN110922218A (en) * | 2019-11-20 | 2020-03-27 | 浙江工业大学 | Composting method for effectively passivating heavy metals in livestock and poultry manure |
| CN111908756A (en) * | 2020-07-29 | 2020-11-10 | 同济大学 | Treatment method for efficiently and stably treating municipal sludge |
| WO2020226351A1 (en) * | 2019-05-03 | 2020-11-12 | 주식회사 원진 | Hybrid biochar and manufacturing method therefor |
-
2021
- 2021-03-31 CN CN202110345955.0A patent/CN113075345B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5583045A (en) * | 1995-01-30 | 1996-12-10 | Bedminster Bioconversion Corporation | Compost curing and odor control system |
| JP2003090839A (en) * | 2001-09-18 | 2003-03-28 | Toru Ueda | Endocrine disrupting substance group and method for evaluating biotic waste processed product using its activity as indicator |
| CN101097211A (en) * | 2006-06-28 | 2008-01-02 | 宝山钢铁股份有限公司 | Method for detecting molecular weight of strong-polarity organic matters in coking wastewater biochemical treatment discharge liquid |
| CN101774828A (en) * | 2010-01-28 | 2010-07-14 | 浙江省农业科学院 | Composting method for biologically dehydrating livestock manure |
| WO2014143741A1 (en) * | 2013-03-15 | 2014-09-18 | Gaia Institute | A method for composting organic waste |
| CN104355711A (en) * | 2014-11-03 | 2015-02-18 | 同济大学 | Aerobic composting method for animal dung with humus |
| CN104829075A (en) * | 2015-04-28 | 2015-08-12 | 上海元钧环保科技有限公司 | Fermentation rotten degree judging method based on humus content |
| JP2017160100A (en) * | 2016-03-11 | 2017-09-14 | トヨタ自動車株式会社 | Method of producing compost in which germination inhibitor is reduced |
| CN107236685A (en) * | 2017-05-22 | 2017-10-10 | 中国科学院成都生物研究所 | A kind of method that utilization sludge produces γ polyglutamic acid organic fertilizers |
| CN108530648A (en) * | 2018-03-13 | 2018-09-14 | 天津大学 | A kind of extraction and purification method of sludge composting fulvic acid |
| WO2020226351A1 (en) * | 2019-05-03 | 2020-11-12 | 주식회사 원진 | Hybrid biochar and manufacturing method therefor |
| CN110117565A (en) * | 2019-05-28 | 2019-08-13 | 青岛力力惠生物科技股份有限公司 | A kind of biology decomposing agent and its application in compost |
| CN110627543A (en) * | 2019-10-30 | 2019-12-31 | 江苏省农业科学院 | Method for promoting compost maturity of livestock and poultry manure through microbial pretreatment |
| CN110922218A (en) * | 2019-11-20 | 2020-03-27 | 浙江工业大学 | Composting method for effectively passivating heavy metals in livestock and poultry manure |
| CN111908756A (en) * | 2020-07-29 | 2020-11-10 | 同济大学 | Treatment method for efficiently and stably treating municipal sludge |
Non-Patent Citations (2)
| Title |
|---|
| S. GREGO ET AL.: "Changes in the Apolar Organic Fraction Through The Composting Process", 《COMPOST SCIENCE & UTILIZATION》 * |
| 李丹 等: "紫外-可见光谱研究堆肥水溶性有机物不同组分演化特征", 《中国环境科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113075345B (en) | 2022-06-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | Selection of sensitive seeds for evaluation of compost maturity with the seed germination index | |
| Selim et al. | Evaluation of phytotoxicity of compost during composting process | |
| Liu et al. | Measurement of cow manure compost toxicity and maturity based on weed seed germination | |
| Raj et al. | Evaluation of maturity and stability parameters of composts prepared from agro-industrial wastes | |
| Gao et al. | The effect of aeration rate on forced-aeration composting of chicken manure and sawdust | |
| Zmora-Nahum et al. | Dissolved organic carbon (DOC) as a parameter of compost maturity | |
| Singh et al. | Solid waste management of temple floral offerings by vermicomposting using Eisenia fetida | |
| Ernst et al. | C and N turnover of fermented residues from biogas plants in soil in the presence of three different earthworm species (Lumbricus terrestris, Aporrectodea longa, Aporrectodea caliginosa) | |
| Wang et al. | Evolution of phytotoxicity during the active phase of co-composting of chicken manure, tobacco powder and mushroom substrate | |
| Martinez-Balmori et al. | Molecular characteristics of vermicompost and their relationship to preservation of inoculated nitrogen-fixing bacteria | |
| Kong et al. | Dissolved organic matter evolution can reflect the maturity of compost: Insight into common composting technology and material composition | |
| Rajbanshi et al. | Chemical and biochemical changes during laboratory-scale composting of allelopathic plant leaves (Eupatorium adenophorum and Lantana camara) | |
| Gao et al. | Ending composting during the thermophilic phase improves cultivation substrate properties and increasing winter cucumber yield | |
| Ciavatta et al. | Compost production, analysis and applications in agriculture | |
| Tietjen et al. | Reducing peat in substrate mixture formulations for press pots using the Taguchi method | |
| CN113075345B (en) | Method for analyzing and determining whether aerobic high-temperature compost is decomposed stably | |
| Nadjet et al. | Effect of three types of composts of olive oil by-products on growth and yield of hard wheat" Triticum durum Desf.” | |
| CN110257486B (en) | Method for characterizing compost maturity based on cellulase gene | |
| Dhimal et al. | Effectiveness of vermicomposting in management of organic wastes using Eisenia foetida and Perionyx favatus in central zoo Jawalakhel, Nepal | |
| Halder et al. | Establishing a method to evaluate the maturity of liquid fertilizer by liquid fertilizer germination index (LFGI) | |
| Vukobratović et al. | Composting of wheat straw by using sheep manure and efective microorganisms | |
| CN101551370A (en) | Method for autointoxication evaluation of agriculture of Chinese medicinal crops | |
| Borang et al. | Effect of various substrates on performance of earthworm and quality of vermicompost | |
| Klimas et al. | Presence of plant hormones in composts made from organic fraction of municipal solid waste | |
| Liu et al. | Effects of applying recycled urban green waste compost made from pruning materials to soil on the growth of plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |