CN113057329A - Glucoraphanin functional food composition - Google Patents

Glucoraphanin functional food composition Download PDF

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Publication number
CN113057329A
CN113057329A CN202110379897.3A CN202110379897A CN113057329A CN 113057329 A CN113057329 A CN 113057329A CN 202110379897 A CN202110379897 A CN 202110379897A CN 113057329 A CN113057329 A CN 113057329A
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functional
glucoraphanin
oligosaccharide
dietary fiber
sugar
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洪汉君
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CHENGDU SANHERB BIOTECH CO LTD
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
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Abstract

The invention provides a glucoraphanin functional confectionary composition with a health-care effect, which comprises a glucoraphanin extract and functional sugar, wherein the functional sugar consists of functional dietary fibers, functional oligosaccharides and functional sugar alcohols; the food composition comprises 100-300 parts by weight of glucoraphanin extract, 100-300 parts by weight of functional dietary fiber, 0.015-0.1 part by weight of functional oligosaccharide and 5-30 parts by weight of functional sugar alcohol. The food composition can be used for preparing functional food with specific nutrition and health promotion functions.

Description

Glucoraphanin functional food composition
Technical Field
The invention relates to a food composition, in particular to a glucoraphanin functional confectionary composition, and belongs to the field of functional foods.
Background
A global survey by the world health organization indicates that truly healthy people account for only 5% of the population, that people with disease account for 20%, and that 75% are in sub-health. In 2004, 740 million cancer deaths worldwide accounted for about 13% of all deaths. WHO expects this number to continue to increase, and it is estimated that 1200 million people will die from cancer in 2030. The statistical data of the China Ministry of health show that: the total number of newly-increased tumor patients in China is about 212.7 million each year, and about 106 million newly-increased malignant tumor patients exist each year. The immune system is the natural barrier of the human body, can generate the immune protection function of maintaining the physiological balance of the organism and stabilizing the anti-tumor and the like, and the harmony of the immune system of the human body is the main reason of the natural regression of the cancer. Long-term hypoimmunity can lead to the development of cancer, and cancer patients are all the people with very low immunity.
The sulforaphane is used as aliphatic glucosinolate, is a natural active substance with the functions of enhancing immunity and resisting cancer, and has the maximum content of 20-50 mg/g in broccoli seeds. Therefore, the broccoli seed extract as a natural anti-cancer substance for enhancing immunity becomes a hot point for research of scholars at home and abroad. In 2017, 13-20% of broccoli seed aqueous extract containing sulforaphane is approved by the national committee of health care to be used as a new food raw material.
In recent years, with the continuous improvement of living standard of people, the dietary structure and the dietary habit of people are changed greatly. The intake of high calorie, high protein, high fat and fine food is greatly increased, and the intake of dietary fiber is relatively reduced. People neglect the balance of dietary nutrition, so that 'rich and noble diseases' -diabetes, cardiovascular diseases, obesity, intestinal cancer, constipation and the like are more and more common. Dietary fiber has been considered as a crude fiber having no nutritional value, but since the dietary fiber hypothesis proposed by burkittttrowell in the 70 th 20 th century, a number of studies have shown that dietary fiber has significant preventive and therapeutic effects on the above-mentioned various diseases. Dietary fiber is widely appreciated by experts from different fields, including medical scientists, dieticians, food scientists, biochemicals, and scientific deciders involved in food regulation and nutrition education, and is listed as the "seventh macronutrient" following carbohydrates, proteins, fats, water, minerals and vitamins. Many experts believe that fiber food will be one of the leading foods in the 21 st century.
The functional oligosaccharide is formed by polymerizing 2-10 monosaccharides through glycosidic bonds, cannot be degraded by gastric acid and gastric enzyme of a human body, cannot be absorbed by small intestines and can directly enter large intestines. The functional oligosaccharide can promote intestinal peristalsis, inhibit harmful bacteria, promote reproduction of probiotics and moisten intestines. With the rapid development of economy, people's demand for food is not only in the stage of satisfying satiety, and food with low energy, anti-caries, probiotic and other functions is more and more favored by consumers.
A functional sugar alcohol is a polyol that contains more than two hydroxyl groups and has some of the attributes of a sugar, although not a sugar. The functional sugar alcohol has higher stability to acid and heat, is not easy to generate Maillard reaction, is used as a low-calorific-value food sweetening agent, and is widely applied to sugar-free functional food formulas. Sugar alcohols have been used abroad in the food industry as sugar substitutes. Sugar alcohol is a good material for preventing dental caries because it is not utilized by microorganisms in the oral cavity. The sugar alcohol has no influence on the blood sugar value rise of a human body, and can provide a certain amount of heat for a diabetic, so the sugar alcohol can be used as a nutritive sweetener for the diabetic and the like. Sugar alcohols are now one of the unlimited use safe food products approved by the international food and health organization.
The inventor surprisingly discovers in research that the glucoraphanin functional sugar food composition combining the glucoraphanin extract and functional sugars such as functional dietary fibers, functional oligosaccharides, functional sugar alcohols and the like has the functions of remarkably improving the immunity and assisting in resisting tumors, has excellent effects and can be used for preparing functional foods with specific nutritional health-care functions.
Disclosure of Invention
The invention provides a glucoraphanin functional confectionary product composition, which is realized by the following technical scheme:
a glucoraphanin functional confectionery composition is provided, which includes a glucoraphanin extract and a functional sugar.
The functional sugar comprises functional dietary fiber, functional oligosaccharide and functional sugar alcohol.
The functional dietary fiber is at least one selected from soybean dietary fiber, seaweed dietary fiber, inulin, natural resistant starch, pectin, konjac polysaccharide, guar gum and chitin, the functional oligosaccharide is at least one selected from stachyose, raffinose, isomaltulose, lactulose, fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, gentiooligosaccharide, soybean oligosaccharide and chitosan oligosaccharide, and the functional sugar alcohol is at least one selected from sorbitol, maltitol, xylitol, erythritol and isomalt.
In some embodiments, the glucoraphanin functional sugar food composition comprises the following ingredients in parts by weight:
Figure BDA0003012550120000021
further, the glucoraphanin functional sugar food composition comprises the following components in parts by weight:
Figure BDA0003012550120000022
Figure BDA0003012550120000031
furthermore, the glucoraphanin functional sugar food composition comprises the following components in parts by weight:
Figure BDA0003012550120000032
in some embodiments, the functional dietary fiber is soy dietary fiber, the functional oligosaccharide is stachyose, and the functional sugar alcohol is xylitol.
The glucoraphanin extract is obtained by extracting broccoli seeds and the glucoraphanin extract according to the proportion of 10-30: 1, and preferably the proportion of 20: 1.
The glucoraphanin functional sugar food composition has the functions of obviously improving the immunity and assisting in resisting tumors, and can be used for preparing functional food with specific nutrition and health-care functions.
The glucoraphanin functional sugar food composition provided by the invention has the advantages that glucoraphanin extract is used as a bidirectional regulator of the immune function, functional oligosaccharide is used as a microecological balance regulator, functional dietary fiber is used as a nutrition enhancer, and functional sugar alcohol is used as a low-calorific-value food sweetening agent, so that the immune function of the organism is jointly improved, the cancer prevention and anti-cancer capacity is assisted and enhanced, the immunity of the organism is enhanced through the synergistic effect from multiple aspects by the composition of a plurality of obvious functional factors, and the harmonious immune system of the organism is maintained.
The product of the invention has the following characteristics: 1) the compatibility is scientific: the glucoraphanin extract, the functional dietary fiber, the functional oligosaccharide and the functional sugar alcohol are scientifically combined. 2) The components are clear and the effect is definite: the product is rich in a plurality of bioactive substances such as glucoraphanin, functional dietary fiber, functional oligosaccharide, functional sugar alcohol and the like, and has definite effect. 3) Natural, safe and has no side effect.
The present invention is described in further detail below with reference to specific embodiments of examples, but it should not be construed that the present invention is limited to the examples, and the present invention based on the above description is within the scope of the present invention.
Detailed Description
Example 1: preparation of glucoraphanin functional sugar granules
The formula is as follows: 100mg of stachyose, 300g of glucoraphanin extract (broccoli seed 10:1), 300g of soybean dietary fiber and 30g of xylitol;
auxiliary materials: 400g of soluble starch and 450g of powdered sugar;
the preparation method comprises the following steps: mixing the above components with adjuvants such as soluble starch and sugar powder, granulating with water as binder, drying, grading to obtain food granule, and packaging to obtain 1.5 g/bag.
The taking method comprises the following steps: 1 bag each time and 1 time daily.
Example 2: preparation of glucoraphanin oral liquid
The formula is as follows: 100mg of stachyose, 80g of glucoraphanin extract (broccoli seeds 30:1), 170g of soybean dietary fiber and 10g of maltitol;
auxiliary materials: 600g of honey, 120g of xylitol and a proper amount of water;
the preparation method comprises the following steps: adding the above formula components and adjuvants into 5000ml water under stirring, heating to dissolve, filtering, adding water to 10000ml, sterilizing, and packaging into 10ml per bottle to obtain oral liquid preparation for food.
The taking method comprises the following steps: 1 daily, 1 branch each time.
Example 3: preparation of glucoraphanin functional sugar tablets
The formula is as follows: 60mg of stachyose, 200g of glucoraphanin extract (broccoli seed 20:1), 170g of soybean dietary fiber and 15g of xylitol;
auxiliary materials: 580g of dextrin, 40g of microcrystalline cellulose and 10g of magnesium stearate;
the preparation method comprises the following steps: mixing the above formula components, sieving with a 40 mesh sieve, adding dextrin and microcrystalline cellulose, mixing well, granulating with 50% ethanol as binder, drying, grading, adding magnesium stearate, mixing well, tabletting, and making into 0.25 g/tablet to obtain the glucoraphanin functional sugar tablet.
The taking method comprises the following steps: 2 tablets each time and 2 times daily.
Example 4: preparation of glucoraphanin functional sugar tablets
The formula is as follows: 60mg of stachyose, 200g of glucoraphanin extract (30: 1 of broccoli seeds), 170g of soybean dietary fiber and 15g of xylitol;
auxiliary materials: 288g of microcrystalline cellulose, 300g of lactose and 12g of magnesium stearate;
the preparation method comprises the following steps: mixing the above formula components, sieving with a 40 mesh sieve, adding microcrystalline cellulose, lactose, magnesium stearate and other adjuvants, mixing well, directly tabletting the powder, and making into 0.25 g/tablet to obtain food tablet.
The taking method comprises the following steps: 2 tablets each time and 2 times daily.
Example 5: functional test
The purpose of the test is as follows: checking whether the product has the function of enhancing immunity
Test materials:
sample preparation: the glucoraphanin prepared in example 3 is orally administered in a recommended amount of 0.25 g/tablet 2 times a day, 2 tablets each time. The adult body weight is calculated according to 60kg, and the equivalent dose is 0.017 g/kg-bw.
Test animals: 200 female mice of clean-grade Kunming species, with the weight of 18-22 g.
The test environmental conditions are as follows: a barrier system. The temperature is 22-24 ℃, and the humidity is 52-58%.
Dose selection and sample treatment: the low, medium and high dosages of the glucoraphanin functional sugar tablet prepared in example 3 were 0.08g/kg · bw, 0.17g/kg · bw and 0.50g/kg · bw, respectively (corresponding to 5, 10 and 30 times of the recommended dosage for human body). When preparing low, medium and high dose test solutions, 0.8g, 1.7g and 5.0g of the glucoraphanin functional sugar tablets prepared in the embodiment 3 are respectively added with distilled water to be constant volume to 200mL, the same volume of distilled water is added to a control group, the test animals are respectively subjected to intragastric gavage, the intragastric gavage is performed once a day, the intragastric volume is 0.2mL/kg · bw, and the continuous 30 days are carried out.
Main apparatus and reagents: an animal platform scale, an analytical balance, a clean bench, a carbon dioxide incubator, an autoclave, a filter, a centrifuge, a 722 spectrophotometer, a constant temperature water bath, a microplate reader, a microscope and the like.
The test method comprises the following steps: the functional evaluation was carried out by organ/body weight ratio measurement, delayed type allergy, ConA-induced lymphocyte transformation test in mice, antibody-producing cell detection, measurement of median hemolysis value, carbon clearance test in mice, phagocytosis of chicken erythrocytes by macrophages in mouse abdominal cavity, and NK cell activity measurement test.
And (3) test results:
when the glucoraphanin prepared in example 3 is orally administered to mice at doses of 0.08 g/kg/bw, 0.17 g/kg/bw and 0.50 g/kg/bw for 30 days, the doses of 0.17 g/kg/bw and 0.50 g/kg/bw can enhance delayed-type allergic reaction of the mice, increase the number of antibody-producing cells of the mice and increase the activity of NK cells of the mice, and the dose of 0.50 g/kg/bw can increase the carbon clearance capacity of mononuclear-macrophages of the mice, increase the lymphocyte transformation capacity of the mice and increase the half hemolysis value of serum of the mice, wherein P is less than 0.05. No significant effect was observed on the mouse body weight gain, spleen/body weight ratio, thymus/body weight ratio, ability of macrophages to phagocytose chicken erythrocytes (P > 0.05). Under the experimental condition, the sample is suggested to have the function of enhancing the immunity.
The specific test results data are shown in the following table:
table 1: example 3 Effect of glucoraphanin-A-functional sugar tablets on delayed type allergy (DTH) in mice
Figure BDA0003012550120000051
As can be seen from Table 1, when different doses of the glucoraphanin functional sugar tablets are orally administered to the mice for 30 days, the degree of foot sole swelling of the medium and high dose groups is obviously higher than that of the control group, and the difference is significant (P < 0.05).
Table 2: example 3 Effect of the food Capsule prepared in example 3 on mouse ConA-induced mouse lymphocyte transformation Capacity test
Figure BDA0003012550120000061
As can be seen from Table 2, when the different doses of the glucoraphanin functional sugar tablets are orally administered to the mice for 30 days, the lymphocyte transformation capacity of the mice in the high dose group is obviously higher than that of the mice in the control group, and the difference is significant (P < 0.05).
Table 3: example 3 Effect of the glucoraphanin-A-functional sugar tablets prepared in example 3 on the number of mouse antibody-producing cells
Figure BDA0003012550120000062
As can be seen from Table 3, when the different doses of the glucoraphanin functional sugar tablets were orally administered to the mice for 30 days, the number of antibody-producing cells in the medium and high dose groups was significantly higher than that in the control group, and the difference was significant (P < 0.05).
Table 4: effect of glucoraphanin prepared in example 3 on half-maximal hemolysis value (HC50) of mouse
Figure BDA0003012550120000063
As can be seen from Table 4, when the different doses of the glucoraphanin functional sugar tablets are orally administered to the mice for 30 days, the half hemolysis value of the mice in the high dose group is obviously higher than that of the control group, and the difference is significant (P < 0.05).
Table 5: example 3 Effect of glucoraphanin-functional sugar tablets on mouse monocyte-macrophage carbon clearance
Figure BDA0003012550120000071
As shown in Table 5, when the different doses of the glucoraphanin functional sugar tablets are orally administered to the mice for 30 days, the phagocytosis index of the mice in the high dose group is obviously higher than that of the control group, and the difference is significant (P < 0.05).
Table 6: example 3 Effect of glucoraphanin functional sugar tablets on NK cell Activity in mice
Figure BDA0003012550120000072
As can be seen from Table 6, when the mice are orally administered with different dosages of the glucoraphanin functional sugar tablets for 30 days, the NK cell activity of the mice in the medium and high dose groups is obviously higher than that of the control group, and the difference is significant (P < 0.05).
Example 6: functional test
The purpose of the test is as follows: checking whether the product has the function of auxiliary inhibiting tumor growth
Test materials:
sample preparation: the glucoraphanin prepared in example 4 is orally administered in a recommended amount of 0.25 g/granule 2 times a day, 2 granules each time. The adult body weight is calculated according to 60kg, and the equivalent dose is 0.017 g/kg-bw.
Test animals: 100 male mice of clean-grade Kunming species, with the body weight of 18-22 g.
The test environmental conditions are as follows: a barrier system. The temperature is 22-24 ℃, and the humidity is 52-58%.
The main apparatus is as follows: an animal platform scale, an analytical balance, a clean bench, a carbon dioxide incubator, an autoclave, a centrifuge, a microscope and the like.
The test method comprises the following steps: a suspension of H22 tumor cells (1X 105) was inoculated subcutaneously into the axilla of the forelimb of animals at a rate of 0.2 ml/mouse for a total of 70 mice. After 24h the weights were randomly divided into 7 groups: the test drugs (the glucoraphanin functional sugar tablets prepared in example 4 are 0.08 g/kg-bw and 0.50 g/kg-bw), cyclophosphamide (CY, 0.03 g/kg-bw) and positive control (CY, 0.03 g/kg-bw) are added into groups of 10 test drugs (10 glucoraphanin functional sugar tablets prepared in example 4 are divided into groups and then administered, the test drugs are filled into the stomach, and the test drugs are injected into the abdominal cavity once a day for 10 days and administered every other day. The general status of each group of mice was observed daily. The next day after drug withdrawal, each group of animals was weighed, mice were sacrificed and tumor mass was stripped off, weighed and tumor inhibition rate was calculated.
The specific test results data are shown in the following table:
table 7: EXAMPLE 4 inhibitory Effect of glucoraphanin-A functional sugar tablet prepared in example 4 on mouse H22 tumor
Figure BDA0003012550120000081
Note: (ii) 0.05, 0.01 as compared to tumor control group; compared with the cyclophosphamide group, the delta is less than 0.05, and the delta is less than 0.01.
After tumor inoculation, the number of times of animal activities of the mice in the model group and the positive control group is obviously reduced, the skin is loose, the hair is upright, and the state is poor. The mice with the single test drug group and the combination of cyclophosphamide group have normal activity, smooth skin and hair and weight gain. The positive control medicine can obviously inhibit the growth of tumors, but the weight of animals is slowly increased, and the state is poor. The tested drug has the effect of inhibiting the growth of the H22 tumor when being used alone, and the tumor inhibition rate is 29.6 percent; the effect is strengthened after the composition is used with the chemotherapeutic drug cyclophosphamide, the tumor inhibition rate is 64.8 percent, and the inhibition rate of cyclophosphamide on H22 tumor is 59.8 percent. Meanwhile, the sample can also increase spleen and thymus index of tumor-bearing mice, promote weight gain of the mice, and has obvious difference compared with a tumor control group and a cyclophosphamide group, which indicates that the sample has protective effect on immune organs of the tumor-bearing mice in chemotherapy and has synergistic attenuation effect when being used with chemotherapeutic drugs. Under the experimental condition, the sample is suggested to have the function of assisting in inhibiting the growth of the tumor.

Claims (9)

1. A glucoraphanin functional confectionary composition is characterized by comprising glucoraphanin extract and functional sugar.
2. The glucoraphanin functional confectionery composition of claim 1, wherein the functional sugar comprises functional dietary fiber, functional oligosaccharide and functional sugar alcohol.
3. A glucoraphanin functional confectionery composition according to claim 2, wherein the functional dietary fiber is selected from at least one of soybean dietary fiber, seaweed dietary fiber, inulin, natural resistant starch, pectin, konjac polysaccharide, guar gum, chitin, the functional oligosaccharide is selected from at least one of stachyose, raffinose, isomaltulose, lactulose, fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, gentiooligosaccharide, soybean oligosaccharide, oligochitosan, the functional sugar alcohol is selected from at least one of sorbitol, maltitol, xylitol, erythritol, isomalt.
4. A glucoraphanin functional confectionery composition according to claim 2 or 3, comprising the following ingredients in parts by weight:
Figure FDA0003012550110000011
5. the glucoraphanin functional confectionery composition of claim 4, comprising the following ingredients in parts by weight:
Figure FDA0003012550110000012
6. a glucoraphanin functional confectionary composition of claim 5 comprising the following ingredients in parts by weight:
Figure FDA0003012550110000013
7. a functional glucoraphanin composition according to any one of claims 3-6, characterized in that the functional dietary fiber is soybean dietary fiber, the functional oligosaccharide is stachyose, and the functional sugar alcohol is xylitol.
8. A functional glucoraphanin composition according to any one of claims 1-7, wherein the glucoraphanin extract is extracted from broccoli seeds and the glucoraphanin extract at a ratio of 10-30: 1, preferably 20: 1.
9. Use of the glucoraphanin functional sugar food composition according to any one of claims 1 to 8 for preparing a functional food having a specific nutritional health function.
CN202110379897.3A 2021-04-08 2021-04-08 Glucoraphanin functional food composition Pending CN113057329A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108294211A (en) * 2018-02-02 2018-07-20 云南瑞宝生物科技股份有限公司 A kind of functional solid beverage and preparation method thereof containing glucorphanin
CN111419857A (en) * 2016-06-30 2020-07-17 深圳福山生物科技有限公司 Glucoraphanin composition and application thereof

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN111419857A (en) * 2016-06-30 2020-07-17 深圳福山生物科技有限公司 Glucoraphanin composition and application thereof
CN108294211A (en) * 2018-02-02 2018-07-20 云南瑞宝生物科技股份有限公司 A kind of functional solid beverage and preparation method thereof containing glucorphanin

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Application publication date: 20210702