CN113040363A - Seasoning with gout resisting function prepared by fermenting lactobacillus acidophilus - Google Patents

Seasoning with gout resisting function prepared by fermenting lactobacillus acidophilus Download PDF

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Publication number
CN113040363A
CN113040363A CN202110392817.8A CN202110392817A CN113040363A CN 113040363 A CN113040363 A CN 113040363A CN 202110392817 A CN202110392817 A CN 202110392817A CN 113040363 A CN113040363 A CN 113040363A
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Prior art keywords
seasoning
lactobacillus acidophilus
flavor
enzymolysis
fermentation
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CN202110392817.8A
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Chinese (zh)
Inventor
李昊恩
解万翠
杨锡洪
董秀芳
吕秀林
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Qingdao University of Science and Technology
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Qingdao University of Science and Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/24Synthetic spices, flavouring agents or condiments prepared by fermentation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • A23L27/215Synthetic spices, flavouring agents or condiments containing amino acids heated in the presence of reducing sugars, e.g. Maillard's non-enzymatic browning
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus

Abstract

The invention belongs to the technical field of seasoning production, and discloses a seasoning with an anti-gout function prepared by lactobacillus acidophilus through fermentation. In the examples, compared with the control results, the seafood seasoning prepared by the invention has the function of resisting gout.

Description

Seasoning with gout resisting function prepared by fermenting lactobacillus acidophilus
Technical Field
The invention relates to a functional seafood seasoning prepared by probiotic fermentation, and relates to the field of seasoning production.
Background
The rapid development of Chinese economy and the improvement of the dietary consumption level bring huge market space and development opportunity for the production industry of salty essences and seasonings, the global annual operating amount of the seasoning industry reaches 2180 billion dollars, accounts for about 10 percent of the food industry amount, and is a typical small product and large market. At present, the total value of China is about 1300 billion yuan, which is about 200 billion dollars, and the proportion is less than 10 percent of the total amount of the world condiment market, so the market potential is huge. The seafood seasoning is characterized by fresh taste and rich nutrition, and becomes the main seasoning for food seasoning and freshness enhancement.
A large amount of byproducts are generated in the processing of aquatic products, wherein protein resources are not fully utilized, so that not only is the resources wasted, but also serious environmental pollution is caused. The main method for producing the seafood seasoning by using the aquatic product processing by-products is to take marine protein as a raw material, carry out compound enzymolysis by endogenous enzyme and an external enzyme preparation, improve the contents of delicious peptide, delicious amino acid and a strong flavor agent, namely nucleotide, and produce the seasoning base material with the seafood flavor by carrying out Maillard reaction under the condition of reheating.
Uric acid (C)5H4N4O3) Is a purine metabolite produced endogenously by primates. When the concentration of serum uric acid in the body of a patient exceeds the standard, hyperuricemia can be diagnosed; gout is caused when uric acid is supersaturated to separate out urate and is deposited in a human body. Xanthine Oxidase (XO) is a key enzyme in the last step of uric acid synthesis, and directly regulates the production of uric acid in purine metabolism. Inhibiting XO can effectively inhibit uric acid synthesis, and is the primary treatment method for reducing uric acid level of organisms and reducing inflammation. The health efficacy of microorganisms and their secondary metabolites has received increasing attention from researchers in recent years.
However, most of the existing seafood seasonings are aquatic product raw material enzymolysis liquid, and the seafood seasoning liquid is heated to generate Maillard reaction to increase fragrance, and the seasoning effect is prominent. With the advent of the afterepidemic era, functional foods and healthy diets have become a trend of development. The functionality of the probiotic fermentation product is well known to consumers, and whether the seafood seasoning can be endowed with functional activity is a key technical problem to be solved by the invention.
Disclosure of Invention
In order to solve the problems that the existing seafood seasoning only focuses on the seasoning effect, does not have obvious functional activity and limits the development of health food, the invention provides a probiotic fermentation method to increase the anti-gout activity of the seafood seasoning, and solves the problems that gout is high in coastal areas and the health of consumers is seriously influenced.
In order to solve the social problems, the invention adopts the following technical scheme:
adding water into a byproduct of the processing of the aquatic product, pulping the cod steak according to a certain material-liquid ratio, adding neutral protease and flavor enzyme for enzymolysis, adding flavor enhancers such as reducing sugar and the like, heating, performing Maillard reaction for flavoring, cooling to room temperature, adding lactobacillus acidophilus for fermentation, and preparing the seafood fish seasoning with the function of resisting uric acid.
The cod steak is adopted as a raw material, water is added according to a certain feed-liquid ratio of 1: 5-1: 10 for pulping, 0.25-0.5% of neutral protease and 0.5-1.00% of flavor enzyme are added according to the mass of cod steak solid, the enzymolysis temperature is 40-55 ℃, and the enzymolysis time is 4-6 hours. Adding flavor intensifier such as glucose 0.50-1.00%, fructose 0.1-0.5%, taurine 0.05-0.10%, etc. into the enzymolysis liquid, heating to 70-100 deg.C, heating for 3-6 hr for Maillard reaction to enhance flavor, cooling to room temperature, adding Lactobacillus acidophilus 107~108CFU/mL, adjusting the fermentation temperature to be 30-40 ℃, adjusting the fermentation time to be 12-24 hours, and detecting and filling the product.
Drawings
FIG. 1 is a graph showing the inhibition of Xanthine Oxidase (XO) activity.
Detailed Description
The invention discloses a seasoning with anti-gout function prepared by fermenting lactobacillus acidophilus, which can be realized by properly improving process parameters by referring to the content in the text. It is expressly intended that all such similar substitutes and modifications which would be obvious to those skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The following detailed description of the invention refers to specific embodiments thereof for better understanding by those skilled in the art.
Example 1
Taking cod steak which is a byproduct of cod processing, adding water according to the feed-liquid ratio of 1:5, pulping, adding neutral protease which is 0.5% of the mass of cod steak solid and flavor enzyme which is 1.0%, wherein the enzymolysis temperature is 40 ℃, and the enzymolysis time is 4 hours. Adding flavor enhancer such as glucose 0.50%, fructose 0.5%, taurine 0.10%, etc., heating to 70 deg.C for 6 hr for Maillard reaction to enhance flavor, cooling to room temperature, adding Lactobacillus acidophilus 107CFU/mL, adjusting the fermentation temperature to 30 ℃, adjusting the fermentation time to 24 hours, and detecting and filling the product.
The anti-gout function evaluation method comprises the following steps:
the supernatant was added to wells in a 96-well plate and the blank was added to the culture medium without inoculation. Each sample was analyzed in triplicate. Then, 140. mu.L of PBS buffer, 60. mu.L of xanthine oxidase (15mU/mL) enzyme, pre-heating at 37 ℃ for 10min, and then 40. mu.L of xanthine (6mM) were added to each well of the 96-well plate, and the initial absorbance value (OD1) at 290nm was immediately measured. The blank set measures absorbance values (OD 0). After incubation of the 96-well plates at 37 ℃ for 10min, the reaction was stopped by addition of 50. mu.L of 1M HCl and the absorbance value (OD3) was measured again at 290 nm. The blank set measures absorbance values (OD 4).
The XO inhibitory activity (XOI) of each sample was calculated according to the following formula:
Figure BDA0003017417490000021
XOI: XO inhibitory Activity
The product is evaluated, the result is shown in figure 1, the Xanthine Oxidase (XO) activity can be inhibited, and the product can resist gout.
Example 2
Adopting cod fillets as raw materials, adding water according to a certain feed-liquid ratio of 1:10 for pulping, and adding the cod filletsNeutral protease accounting for 0.5 percent of solid matter and flavourzyme accounting for 0.5 percent of solid matter, wherein the enzymolysis temperature is 55 ℃, and the enzymolysis time is 6 hours. Adding flavor enhancer such as glucose 1.00%, fructose 0.1%, taurine 0.05%, etc., heating to 100 deg.C for 3 hr for Maillard reaction to enhance flavor, cooling to room temperature, adding Lactobacillus acidophilus 108CFU/mL, adjusting the fermentation temperature to 40 ℃, adjusting the fermentation time to 12 hours, and detecting and filling the product.
The product is evaluated, the result is shown in figure 1, the Xanthine Oxidase (XO) activity can be inhibited, and the product can resist gout.
EXAMPLE 3
The cod steak is adopted as a raw material, water is added according to a certain feed-liquid ratio of 1:7 for pulping, neutral protease accounting for 0.3% of the mass of the cod steak solid and flavor enzyme accounting for 0.75% of the mass of the cod steak solid are added, the enzymolysis temperature is 50 ℃, and the enzymolysis time is 5 hours. Adding flavor enhancer such as glucose 0.75%, fructose 0.3%, taurine 0.075%, etc., heating to 80 deg.C for 5 hr for Maillard reaction to enhance flavor, cooling to room temperature, adding Lactobacillus acidophilus 107CFU/mL, adjusting the fermentation temperature to be 30-40 ℃, adjusting the fermentation time to be 20 hours, and detecting and filling the product.
The product is evaluated, the result is shown in figure 1, the Xanthine Oxidase (XO) activity can be inhibited, and the product can resist gout.
Control column
The cod steak is adopted as a raw material, water is added according to a certain feed-liquid ratio of 1:5 for pulping, neutral protease accounting for 0.5% of the mass of the cod steak solid and flavor enzyme accounting for 0.75% of the mass of the cod steak solid are added, the enzymolysis temperature is 45 ℃, and the enzymolysis time is 6 hours. Adding flavor intensifier such as glucose 1.00%, fructose 0.21%, taurine 0.10%, etc., heating to 100 deg.C, heating for 3 hr for Maillard reaction to enhance flavor, cooling to room temperature, detecting, and bottling.
The product is evaluated, the result is shown in figure 1, the activity of inhibiting Xanthine Oxidase (XO) is low, and the product has no anti-gout function.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (6)

1. A seasoning with anti-gout function prepared by fermenting lactobacillus acidophilus is characterized in that: adding water into a byproduct of the processing of the aquatic product, pulping the cod steak according to a certain material-liquid ratio, adding neutral protease and flavor enzyme for enzymolysis, adding flavor enhancers such as reducing sugar and the like, heating, performing Maillard reaction for flavoring, cooling to room temperature, adding lactobacillus acidophilus for fermentation, and preparing the seafood fish seasoning with the function of resisting uric acid.
2. The enzymatic hydrolysis of claim 1, wherein: adding neutral protease with the weight percentage of 0.25-0.5% of the solid content of the cod steak and flavor enzyme with the weight percentage of 0.5-1.00%.
3. The enzymatic conditions of claim 1, wherein: the enzymolysis temperature is 40-55 ℃, and the enzymolysis time is 4-6 hours.
4. The flavor enhancer of claim 1, wherein: comprises the following components, by weight, 0.50-1.00% of glucose, 0.1-0.5% of fructose and 0.05-0.10% of taurine.
5. A thermal fragrancing reaction as claimed in claim 1, characterized in that: adding the flavor enhancer enzymolysis liquid, heating to 70-100 ℃, and heating for 3-6 hours.
6. The probiotic fermentation of claim 1, characterized in that: the addition amount of Lactobacillus acidophilus is 107~108CFU/mL, fermentation temperature of 30-40 ℃, and time of 12-24 hours.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115429820A (en) * 2022-05-16 2022-12-06 微康益生菌(苏州)股份有限公司 Application of lactobacillus acidophilus in preparation of product for reducing blood uric acid

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0866165A (en) * 1994-08-30 1996-03-12 Tokushima Pref Gov Production of fermented seasoning using chicken bone as raw material
CN101156673A (en) * 2007-11-21 2008-04-09 中国水产科学研究院南海水产研究所 A method for preparing flavouring base material from java tilapia processing by-product
CN103142655A (en) * 2013-02-28 2013-06-12 薛松晓 Composite probiotics for treating podagra and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0866165A (en) * 1994-08-30 1996-03-12 Tokushima Pref Gov Production of fermented seasoning using chicken bone as raw material
CN101156673A (en) * 2007-11-21 2008-04-09 中国水产科学研究院南海水产研究所 A method for preparing flavouring base material from java tilapia processing by-product
CN103142655A (en) * 2013-02-28 2013-06-12 薛松晓 Composite probiotics for treating podagra and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115429820A (en) * 2022-05-16 2022-12-06 微康益生菌(苏州)股份有限公司 Application of lactobacillus acidophilus in preparation of product for reducing blood uric acid

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