CN113029640A - Freezing formula section device for it is biological experiments - Google Patents

Freezing formula section device for it is biological experiments Download PDF

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Publication number
CN113029640A
CN113029640A CN202110451075.1A CN202110451075A CN113029640A CN 113029640 A CN113029640 A CN 113029640A CN 202110451075 A CN202110451075 A CN 202110451075A CN 113029640 A CN113029640 A CN 113029640A
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CN
China
Prior art keywords
groove
freezing
support frame
fixedly installed
depth control
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Pending
Application number
CN202110451075.1A
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Chinese (zh)
Inventor
吕然然
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Individual
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Individual
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Priority to CN202110451075.1A priority Critical patent/CN113029640A/en
Publication of CN113029640A publication Critical patent/CN113029640A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/04Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42Low-temperature sample treatment, e.g. cryofixation

Abstract

The invention provides a freezing type slicing device for a biological experiment, and relates to the field of biological experiment equipment. This frozen formula section device for it is biological experiments, including the support frame, the top the place ahead fixed mounting of support frame has degree of depth control mechanism, and the freezing mechanism of the place ahead fixed mounting of support frame freezes the mechanism, freezes the mechanism and is located degree of depth control mechanism under, and one side fixed mounting that the top of support frame is located degree of depth control mechanism has power unit. This frozen type section device for it is biological experiments, can drive the rack rotation through pressing the control gasbag, thereby make in the weight ball weight of a certain amount falls to the inner skleeve, change how much that biological sample immerges in the freezing groove through the weight ball, the cooperation freezing groove freezes biological sample, will freeze through the scraper blade after freezing and obtain biological sample and separate with the biological sample that does not freeze, thereby can solve among the prior art biological sample section thickness wayward and the problem of the fold of the easy play of section in-process.

Description

Freezing formula section device for it is biological experiments
Technical Field
The invention relates to the technical field of biological experiment equipment, in particular to a freezing type slicing device for a biological experiment.
Background
Biological experiments refer to the process of purposefully observing and researching the phenomena of organism structure and life activities which are not easy to observe under general conditions by a scientific method by using certain instruments, materials and medicines under specific environmental conditions. Biology is a natural science based on experiments. Through biological experiments, the system not only can help students to understand concepts and rules of biology and really learn basic knowledge of biology, but also is beneficial to inspiring active thinking of students, carrying out scientific method training and cultivating scientific quality of students.
When the current slicing observation is carried out on biological tissues, the thickness of the biological slices in the current slicing process is not easy to control, and the surfaces of the slices are easy to wrinkle in the slicing process, so that a novel biological tissue slicing device is needed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a freezing type slicing device for a biological experiment, which solves the problems in the background technology.
In order to achieve the purpose, the invention is realized by the following technical scheme: the freezing type slicing device for the biological experiment comprises a support frame, wherein a depth control mechanism is fixedly arranged in front of the top end of the support frame, a freezing mechanism is fixedly arranged in front of the support frame and is positioned under the depth control mechanism, a power mechanism is fixedly arranged on one side of the depth control mechanism above the support frame, a counterweight mechanism is meshed with the top end of the power mechanism and is positioned above the depth control mechanism, the counterweight mechanism is fixedly arranged on the side of the depth control mechanism, the depth control mechanism comprises an outer sleeve, the outer sleeve is fixedly arranged in front of the top end of the support frame, a through groove is formed in the front end of the outer sleeve, a baffle is connected in the through groove in a sliding manner, a reset spring is fixedly arranged on the inner bottom wall of the outer sleeve, an inner sleeve is fixedly arranged on the top, the front end of the inner sleeve is provided with a middle groove, the bottom end of the inner sleeve is fixedly provided with a support rod, the bottom end of the support rod penetrates through the outer sleeve and extends to the lower part of the outer sleeve, two sides of the outer surface of the support rod, which is positioned below the outer sleeve, are fixedly provided with limit blocks, two sides of the outer surface of the outer sleeve are fixedly provided with limit sleeves, the limit blocks are connected inside the limit sleeves in a sliding manner, the bottom end of the support rod is fixedly provided with a fixing frame, the inner top wall of the fixing frame is fixedly provided with an upper fixing needle, the side of the fixing frame is provided with a through hole, the inside of the through hole is inserted with a side fixing needle, the freezing mechanism comprises a freezing groove, the freezing groove is fixedly arranged at the front end of the support frame, the middle part of, pipe casing fixed mounting is at the upper surface of support frame, and the inside sliding connection of pipe casing one end has the kickboard, and the top fixed mounting of kickboard has the rack board, and the top of rack board extends to the top of pipe casing, and the one end fixed mounting that the kickboard was kept away from to the pipe casing has the control gasbag, counter weight mechanism includes the shell, and the top of shell extends to the top of inner skleeve, and the shell passes through link and outer sleeve fixed connection, and the bottom and the top fixed rotation of shell are connected with drive gear, and the drive gear one end fixed mounting of bottom has power gear, and power gear and rack board meshing, drive gear's surface has cup jointed the rack area, and the arrangement groove has been seted up to the surface of rack area, and the counter weight ball has been placed to the inside in arrangement groove.
Preferably, the section of the pipe groove is U-shaped, an observation window is fixedly arranged in front of the pipe groove, and a sliding plate is connected in the pipe groove in a sliding manner.
Preferably, a tension wheel is fixedly mounted inside the housing, the tension wheel is sleeved outside the toothed belt, and a blocking groove is fixedly mounted on one side, located above the outer sleeve, of the top end of the housing.
Preferably, the inner bottom wall of the inner sleeve is U-shaped, the middle groove is flush with the through groove, and the width of the middle groove is the same as that of the through groove.
Preferably, the distance between the placing grooves is the same, and the diameter of the placing grooves is the same as that of the counterweight balls.
Preferably, the thread below the outer surface of the limiting sleeve is connected with a fixing bolt, and the inner end of the fixing bolt is in contact with the limiting block.
The invention has the following beneficial effects:
1. this freezing formula section device for it is biological experiments, sheathed tube cross sectional shape are "U" font, and sheathed tube preceding fixed mounting has the observation window, and sheathed tube inside sliding connection has the sliding plate, through setting to "U" shape, can save space, can observe liquid displacement through setting up observation window and sliding plate, has solved among the prior art problem that the section displacement is little difficult for observing down.
2. This frozen type section device for it is biological experiments, the interval between the resettlement groove is the same, and the diameter of resettlement groove is the same with the diameter of counter weight ball to and set up and place the mouth and can be convenient for refill the resettlement groove with the counter weight ball after finishing using, solved among the prior art can not recycle the problem.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic top half-section view of the depth control mechanism of the present invention;
FIG. 3 is a bottom view of the depth control mechanism of the present invention;
FIG. 4 is a schematic half-sectional view of a freezing mechanism according to the present invention;
FIG. 5 is a schematic half-sectional view of a power mechanism according to the present invention;
FIG. 6 is a schematic half-sectional view of a counterweight mechanism according to the present invention.
Wherein, the support frame-1, the depth control mechanism-2, the freezing mechanism-3, the power mechanism-4, the counterweight mechanism-5, the outer sleeve-201, the through groove-202, the baffle-203, the reset spring-204, the inner sleeve-205, the support rod-206, the limit block-207, the limit sleeve-208, the fixed mount-209, the upper fixed pin-210, the through hole-211, the side fixed pin-212, the middle groove-213, the fixed bolt-214, the freezing groove-301, the cover plate-302, the liquid inlet-303, the placing groove-304, the scraper-305, the pipe groove-401, the floating plate-402, the rack plate-403, the control air bag-404, the observation window-405, the sliding plate-406, the shell-501, the connecting frame-502, the power mechanism-4, a transmission gear-503, a power gear-504, a rack belt-505, a placing groove-506, a counterweight ball-507, a placing opening-508 and a tension wheel-509.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides a freezing type slicing device for a biological experiment, which comprises:
in the first embodiment, as shown in fig. 1 to 6, the depth control mechanism comprises a support frame 1, a depth control mechanism 2 is fixedly installed in front of the top end of the support frame 1, a freezing mechanism 3 is fixedly installed in front of the support frame 1, the freezing mechanism 3 is located right below the depth control mechanism 2, a power mechanism 4 is fixedly installed above the support frame 1 and on one side of the depth control mechanism 2, a counterweight mechanism 5 is engaged with the top end of the power mechanism 4, the counterweight mechanism 5 is located above the depth control mechanism 2, the counterweight mechanism 5 is fixedly installed on the side of the depth control mechanism 2, the depth control mechanism 2 comprises an outer sleeve 201, the outer sleeve 201 is fixedly installed in front of the top end of the support frame 1, a through groove 202 is formed in the front end of the outer sleeve 201, a baffle 203 is slidably connected inside the through groove 202, a return spring 204 is fixedly installed, an inner sleeve 205 is slidably connected inside the outer sleeve 201.
The front end of the inner sleeve 205 is provided with a middle groove 213, the inner bottom wall of the inner sleeve 205 is U-shaped, the middle groove 213 is flush with the through groove 202, and the width of the middle groove 213 is the same as that of the through groove 202, so that the counterweight ball 507 can be conveniently recycled.
The bottom end of the inner sleeve 205 is fixedly provided with a support rod 206, the bottom end of the support rod 206 penetrates through the outer sleeve 201 and extends to the lower portion of the outer sleeve 201, the two sides of the outer surface of the support rod 206 below the outer sleeve 201 are fixedly provided with limit blocks 207, the two sides of the outer surface of the outer sleeve 201 are fixedly provided with limit sleeves 208, the lower portion of the outer surface of the limit sleeves 208 is in threaded connection with fixing bolts 214, the inner ends of the fixing bolts 214 are in contact with the limit blocks 207, and through the arrangement, the support rod 206 can be positioned when the support rod.
The limiting block 207 is connected inside the limiting sleeve 208 in a sliding manner, the bottom end of the supporting rod 206 is fixedly provided with a fixing frame 209, the inner top wall of the fixing frame 209 is fixedly provided with an upper fixing needle 210, the side of the fixing frame 209 is provided with a through hole 211, the inside of the through hole 211 is inserted with a side fixing needle 212, a biological tissue sample can be fixed by arranging the side fixing needle 212 and the upper fixing needle 210, the freezing mechanism 3 comprises a freezing groove 301, the inside of the freezing groove 301 is hollow, the freezing groove 301 is fixedly arranged at the front end of the supporting frame 1, the middle part of the freezing groove 301 is inserted with a cover plate 302, the cover plate 302 has a sealing effect, one side of the freezing groove 301 is connected with and penetrated with a liquid inlet 303, the front end of the freezing groove 301 is fixedly provided with a placing groove 304, the inside of the placing groove 304 is clamped with a scraping plate 305, the power mechanism 4 comprises a, the cross-sectional shape of the tube tray 401 is "U" shape, the observation window 405 is fixedly installed on the front surface of the tube tray 401, the sliding plate 406 is slidably connected to the inside of the tube tray 401, the diameter of the sliding plate 406 is the same as that of the tube tray 401, the U shape is set, space can be saved, and the moving distance of the liquid can be observed by setting the observation window 405 and the sliding plate 406.
The inside sliding connection of tube seat 401 one end has floating plate 402, the top fixed mounting of floating plate 402 has rack plate 403, the top of rack plate 403 extends to the top of tube seat 401, the one end fixed mounting that floating plate 402 was kept away from to tube seat 401 has control gasbag 404, counter weight mechanism 5 includes shell 501, the top of shell 501 extends to the top of inner skleeve 205, shell 501 passes through link 502 and outer sleeve 201 fixed connection, the bottom and the top fixed rotation of shell 501 are connected with drive gear 503, the drive gear 503 one end fixed mounting of bottom has power gear 504.
The power gear 504 is meshed with the rack plate 403, the toothed belt 505 is sleeved on the outer surface of the transmission gear 503, the tension wheel 509 is fixedly mounted inside the shell 501, the tension wheel 509 is sleeved on the outer side of the toothed belt 505, the blocking groove is fixedly mounted on one side, located above the outer sleeve 201, of the top end of the shell 501, the toothed belt 505 can be in a tensioned state through the arrangement of the tension wheel 509, and therefore the toothed belt 505 can be made to be L-shaped so that a plurality of installation grooves can be formed conveniently.
The outer surface of the rack belt 505 is provided with a placing groove 506, a counterweight ball 507 is placed in the placing groove 506, the mass of the counterweight ball 507 is the same, the distance between the placing grooves 506 is the same, the diameter of the placing groove 506 is the same as that of the counterweight ball 507, and through the limitation, the power gear 504 can be guaranteed to rotate for one circle, the falling number of the counterweight balls 507 is the same, and further the falling number of the counterweight balls 507 and the moving position of the sliding plate 406 are in linear relation. The surface of the housing 501 is provided with a placing opening 508 at the position of the placing groove 506.
When the biological sample freezing device is used, a biological sample is inserted into the upper fixing needle 210, the side fixing needle 212 is used for fixing the biological surface, the pressing control air bag 404 can ventilate the inside of the pipe groove 401, the liquid in the pipe groove 401 is pressed to move through air, the rack plate 403 can be driven to move, the rack belt 505 can be driven to move through gear transmission, the counterweight ball 507 outside the rack belt 505 can be pushed, when the counterweight ball 507 moves to the top end, the counterweight ball can slide into the inner sleeve 205, the inner sleeve 205 is driven to move downwards through pressing the inner sleeve 205, the depth of the biological sample extending into the freezing groove 301 can be controlled, the sliding plate 406 can also move in the moving process of the floating plate 402, the dropping amount of the counterweight ball 507 can be calculated through observing the position of the sliding plate 406, liquid nitrogen is led into the freezing groove 301 through the liquid inlet 303, and the temperature inside the freezing groove 301 is reduced, the biological sample is partially frozen, after freezing is finished, the scraper 305 slides over the unfrozen part and the frozen part of the biological sample to separate the frozen part from the unfrozen part, so that the slice thickness of the biological sample can be controlled, meanwhile, the problem of wrinkling in the slicing process can be avoided, after the biological sample is used, the counterweight ball 507 can be taken out of the inner sleeve 205 by opening the baffle plate 203, and the rack belt 505 is driven to rotate by pulling the rack plate 403, so that the counterweight ball 507 can be placed in the placement groove 506.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. The utility model provides a frozen formula section device for it is biological experiments, includes support frame (1), its characterized in that: a depth control mechanism (2) is fixedly mounted in front of the top end of the support frame (1), a freezing mechanism (3) is fixedly mounted in front of the support frame (1), the freezing mechanism (3) is located right below the depth control mechanism (2), a power mechanism (4) is fixedly mounted on one side, located above the support frame (1), of the depth control mechanism (2), a counterweight mechanism (5) is meshed with the top end of the power mechanism (4), the counterweight mechanism (5) is located above the depth control mechanism (2), and the counterweight mechanism (5) is fixedly mounted on the side of the depth control mechanism (2);
the depth control mechanism (2) comprises an outer sleeve (201), the outer sleeve (201) is fixedly installed in front of the top end of the support frame (1), a through groove (202) is formed in the front end of the outer sleeve (201), a baffle (203) is connected to the inside of the through groove (202) in a sliding manner, a reset spring (204) is fixedly installed on the inner bottom wall of the outer sleeve (201), an inner sleeve (205) is fixedly installed on the top end of the reset spring (204), the inner sleeve (205) is connected to the inside of the outer sleeve (201) in a sliding manner, a middle groove (213) is formed in the front end of the inner sleeve (205), a support rod (206) is fixedly installed at the bottom end of the inner sleeve (205), the bottom end of the support rod (206) penetrates through the outer sleeve (201) and extends to the lower portion of the outer sleeve (201), limiting blocks (207) are fixedly installed on, the limiting block (207) is connected inside the limiting sleeve (208) in a sliding mode, a fixing frame (209) is fixedly installed at the bottom end of the supporting rod (206), an upper fixing needle (210) is fixedly installed on the inner top wall of the fixing frame (209), a through hole (211) is formed in the side of the fixing frame (209), and a side fixing needle (212) is inserted inside the through hole (211);
the freezing mechanism (3) comprises a freezing groove (301), the freezing groove (301) is fixedly installed at the front end of the support frame (1), a cover plate (302) is inserted in the middle of the freezing groove (301), one side of the freezing groove (301) is connected with and penetrates through a liquid inlet (303), a placing groove (304) is fixedly installed at the front end of the freezing groove (301), and a scraper blade (305) is clamped in the placing groove (304);
the power mechanism (4) comprises a pipe groove (401), the pipe groove (401) is fixedly installed on the upper surface of the support frame (1), a floating plate (402) is connected to the inner portion of one end of the pipe groove (401) in a sliding mode, a rack plate (403) is fixedly installed at the top end of the floating plate (402), the top end of the rack plate (403) extends to the upper portion of the pipe groove (401), and a control air bag (404) is fixedly installed at one end, far away from the floating plate (402), of the pipe groove (401);
counter weight mechanism (5) include shell (501), the top of shell (501) extends to the top of inner skleeve (205), shell (501) are through link (502) and outer skleeve (201) fixed connection, the bottom and the top fixed rotation of shell (501) are connected with drive gear (503), drive gear (503) one end fixed mounting of bottom has power gear (504), power gear (504) and rack board (403) meshing, rack belt (505) have been cup jointed to the surface of drive gear (503), resettlement groove (506) have been seted up to the surface of rack belt (505), counter weight ball (507) have been placed to the inside of resettlement groove (506), the surface of shell (501) is located resettlement groove (506) and has been seted up and place mouth (508).
2. The apparatus of claim 1, wherein: the section of the pipe groove (401) is U-shaped, an observation window (405) is fixedly arranged in front of the pipe groove (401), and a sliding plate (406) is connected in the pipe groove (401) in a sliding manner.
3. The apparatus of claim 1, wherein: a tension wheel (509) is fixedly mounted in the shell (501), the tension wheel (509) is sleeved on the outer side of the rack belt (505), and a blocking groove is fixedly mounted on one side, located above the outer sleeve (201), of the top end of the shell (501).
4. The apparatus of claim 1, wherein: the inner bottom wall of the inner sleeve (205) is U-shaped, the middle groove (213) is flush with the through groove (202), and the width of the middle groove (213) is the same as that of the through groove (202).
5. The apparatus of claim 1, wherein: the distance between the placing grooves (506) is the same, and the diameter of the placing grooves (506) is the same as that of the counterweight balls (507).
6. The apparatus of claim 1, wherein: the lower part of the outer surface of the limiting sleeve (208) is in threaded connection with a fixing bolt (214), and the inner end of the fixing bolt (214) is in contact with the limiting block (207).
CN202110451075.1A 2021-04-26 2021-04-26 Freezing formula section device for it is biological experiments Pending CN113029640A (en)

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CN202110451075.1A CN113029640A (en) 2021-04-26 2021-04-26 Freezing formula section device for it is biological experiments

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Application Number Priority Date Filing Date Title
CN202110451075.1A CN113029640A (en) 2021-04-26 2021-04-26 Freezing formula section device for it is biological experiments

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114055547A (en) * 2021-11-29 2022-02-18 达科为(深圳)医疗设备有限公司 Sample calliper
CN115655776A (en) * 2022-07-28 2023-01-31 南通大学附属医院 Pathology department sample freezing slicing system and slicing method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11118679A (en) * 1997-10-15 1999-04-30 Kanagawa Acad Of Sci & Technol Method and apparatus for holding and feeding refrigerated work
CN103975229A (en) * 2011-09-29 2014-08-06 迈阿密大学 Ultra-rapid diagnostic tissue preparation as an alternative to frozen section
CN208701069U (en) * 2018-07-05 2019-04-05 南京路通工程科技有限公司 A kind of microbial metabolism detecting oxygen device
CN109737897A (en) * 2019-03-07 2019-05-10 王子琛 A kind of hydraulic detection device of the plate-girder hinge seam vertical relative displacement in two sides
CN111268364A (en) * 2020-02-11 2020-06-12 衢州图艺工业设计有限公司 Steel ball loading device for ball mill barrel

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11118679A (en) * 1997-10-15 1999-04-30 Kanagawa Acad Of Sci & Technol Method and apparatus for holding and feeding refrigerated work
CN103975229A (en) * 2011-09-29 2014-08-06 迈阿密大学 Ultra-rapid diagnostic tissue preparation as an alternative to frozen section
CN208701069U (en) * 2018-07-05 2019-04-05 南京路通工程科技有限公司 A kind of microbial metabolism detecting oxygen device
CN109737897A (en) * 2019-03-07 2019-05-10 王子琛 A kind of hydraulic detection device of the plate-girder hinge seam vertical relative displacement in two sides
CN111268364A (en) * 2020-02-11 2020-06-12 衢州图艺工业设计有限公司 Steel ball loading device for ball mill barrel

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114055547A (en) * 2021-11-29 2022-02-18 达科为(深圳)医疗设备有限公司 Sample calliper
CN114055547B (en) * 2021-11-29 2023-06-23 达科为(深圳)医疗设备有限公司 Sample calliper
CN115655776A (en) * 2022-07-28 2023-01-31 南通大学附属医院 Pathology department sample freezing slicing system and slicing method

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