CN113018448B - Dipeptide self-assembled fiber coated calcium carbonate and preparation method and application thereof - Google Patents
Dipeptide self-assembled fiber coated calcium carbonate and preparation method and application thereof Download PDFInfo
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- CN113018448B CN113018448B CN202110256716.8A CN202110256716A CN113018448B CN 113018448 B CN113018448 B CN 113018448B CN 202110256716 A CN202110256716 A CN 202110256716A CN 113018448 B CN113018448 B CN 113018448B
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
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Abstract
The invention provides dipeptide self-assembled fiber coated calcium carbonate and a preparation method and application thereof, wherein the preparation method comprises the following steps: (1) Mixing and dissolving calcium chloride and a charged compound, and then mixing the calcium chloride and a carbonate solution to obtain calcium carbonate particles with positive charges or negative charges on the surfaces; (2) And (2) mixing the calcium carbonate particles with positive charges or negative charges on the surface obtained in the step (1) with the dipeptide to obtain the calcium carbonate coated by the dipeptide self-assembly fiber. The calcium carbonate coated by the dipeptide self-assembly fiber provided by the invention has low toxicity and good drug slow-release effect, and is suitable for the field of medical science.
Description
Technical Field
The invention belongs to the field of biological materials, particularly relates to dipeptide self-assembled fiber coated calcium carbonate and a preparation method and application thereof, and particularly relates to low-toxicity dipeptide self-assembled fiber coated calcium carbonate and a preparation method and application thereof.
Background
Calcium carbonate is one of the most common and readily available materials in nature, and is easy to prepare and low in preparation cost. Crystalline calcium carbonate is a white hexagonal crystalline powder having three anhydrous crystalline forms of vaterite, aragonite and calcite. The most common is vaterite-structured calcium carbonate, mainly because the vaterite-structured calcium carbonate particles are stacked by hundreds of nano-sized calcium carbonate, so that CaCO 3 The particles have a porous structure, a high surface area and good dispersibility in aqueous solutions, and are widely used in biomedicine, materials science and the like.
Self-assembly of peptides is very common in nature, and it is composed of amino acids, is derived from organisms, is degradable in vivo, and metabolites are non-toxic, and its research has been receiving much attention in recent years. A large number of bioactive polypeptides exist in nature, have biocompatibility and biodegradability, play a very important role in biological activities, and relate to various fields such as molecular recognition, cell differentiation, signal transduction, cell differentiation, ontogenesis and the like. Because the dipeptide has rich sequence and is easy to modify, the dipeptide can be conveniently combined with other functional groups (such as N-terminal fluorenyl-9-methoxycarbonyl and Fmoc), and further the unique and adjustable self-assembly behavior of the dipeptide is endowed, so that the dipeptide has special appearance and function.
Although the assembly behavior of dipeptides has been extensively studied and has been exciting. It is still difficult to modify the surface finish of the particles to obtain calcium carbonate particles with certain properties on the surface, which are previously desired. Various functional nanostructures can be prepared in the current research, however, the influence of medium surface properties such as surface charge, hydrophobicity and hydrophilicity on self-assembly behaviors has not been studied in detail, so that the deep understanding of an interface on a dipeptide self-assembly mechanism is limited, and the further application of the dipeptide self-assembly mechanism in the medical field is limited.
CN111035768A discloses a calcium carbonate nanoparticle composition for improving oral absorption of insoluble drugs. The composition consists of insoluble drugs, amphiphilic polymers for connecting substrates of transporters expressed by small intestinal epithelial cells, calcium carbonate nanoparticles and optional medicinal auxiliary materials. The composition can obviously improve the oral absorption of insoluble drugs, and provides a novel preparation strategy for the oral administration of the insoluble drugs. But it has the risk of rapid drug release and high toxicity.
At present, no study on dipeptide self-assembly on the surface of the vaterite-structured calcium carbonate particles exists, and a calcium carbonate drug carrier with good drug slow-release effect and low toxicity does not exist. Therefore, how to provide a calcium carbonate drug carrier with good drug slow-release effect and low toxicity becomes a problem to be solved urgently.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide calcium carbonate coated by dipeptide self-assembled fibers, a preparation method and application thereof, and particularly provides calcium carbonate coated by dipeptide self-assembled fibers with low toxicity, a preparation method and application thereof. The calcium carbonate coated by the dipeptide self-assembly fiber provided by the invention has low toxicity and good drug slow-release effect, and is suitable for the field of medical science.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a preparation method of dipeptide self-assembled fiber coated calcium carbonate, which comprises the following steps:
(1) Mixing and dissolving calcium chloride and a charged compound, and then mixing the calcium chloride and a carbonate solution to obtain calcium carbonate particles with positive charges or negative charges on the surfaces;
(2) And (2) mixing the calcium carbonate particles with positive charges or negative charges on the surface obtained in the step (1) with the dipeptide to obtain the calcium carbonate coated by the dipeptide self-assembly fiber.
The preparation method comprises the steps of modifying the surface of calcium carbonate particles, and then adsorbing the calcium carbonate particles with dipeptide in a combined manner, so that the calcium carbonate coated by the dipeptide self-assembly fibers has the characteristics of low toxicity and good drug slow-release effect; the calcium carbonate is nontoxic and can adapt to different human body environments, the specific dipeptide can improve the drug slow release effect, and the specific modified compound can be selected to tightly adsorb the dipeptide on the surface of the calcium carbonate, so that the drug slow release effect of the calcium carbonate coated by the dipeptide self-assembly fiber in a human body is obviously improved.
Preferably, the charged compound of step (1) comprises any one of polydimethyldiallylammonium chloride (PDDA), gelatin, or polystyrene sulfonate (PSS).
The specific modified compound can tightly adsorb the dipeptide, and the drug slow-release effect of the calcium carbonate coated by the dipeptide self-assembly fiber is improved.
Preferably, in step (1), the mass ratio of calcium chloride to charged molecule is 1.
Preferably, the carbonate of step (1) comprises sodium carbonate and/or potassium carbonate.
Preferably, the dipeptide of step (2) comprises Fmoc-YL-NH 2 、Fmoc-LL-NH 2 Or Fmoc-TL-NH 2 Any one of themPreferably Fmoc-YL-NH 2 。
The specific dipeptide can improve the drug slow release effect of the calcium carbonate coated by the dipeptide self-assembly fiber, and has biocompatibility, biodegradability and low toxicity.
Preferably, said Fmoc-YL-NH 2 The preparation method comprises the following steps: fmoc-Y, L-NH 2 Mixing with enzyme and dissolving to obtain Fmoc-YL-NH 2 。
Preferably, the enzyme comprises thermolysin.
Preferably, the step (2) of mixing the calcium carbonate particles having positive or negative charges on the surface obtained in the step (1) with the dipeptide further comprises the step (2') of mixing the calcium carbonate particles having positive or negative charges on the surface obtained in the step (1) with a hydrophilic compound or a hydrophobic compound to obtain calcium carbonate particles having hydrophilic or hydrophobic groups on the surface.
The hydrophilic or hydrophobic compound is used for further modifying the calcium carbonate particles with positive charges or negative charges on the surface, so that the affinity effect of the calcium carbonate coated by the dipeptide self-assembly fibers in a human body environment can be improved, and the drug slow-release effect can be improved.
Preferably, the hydrophilic compound of step (2') comprises dopamine.
Preferably, the hydrophobic compound of step (2') comprises stearic acid.
As a preferred technical scheme of the invention, the preparation method comprises the following steps:
(1) Mixing and dissolving calcium chloride and a charged compound, and then mixing the calcium chloride and a carbonate solution to obtain calcium carbonate particles with positive charges or negative charges on the surfaces;
(2) Mixing the calcium carbonate particles with positive charges or negative charges on the surface obtained in the step (1) with a hydrophilic compound or a hydrophobic compound to obtain calcium carbonate particles with hydrophilic groups or hydrophobic groups on the surface;
(3) And (3) mixing the calcium carbonate particles with positive charges or negative charges on the surface obtained in the step (1) or the calcium carbonate particles with hydrophilic or hydrophobic groups on the surface obtained in the step (2) with the dipeptide to obtain the calcium carbonate coated by the dipeptide self-assembly fiber.
In a second aspect, the invention provides the calcium carbonate coated by the dipeptide self-assembled fiber prepared by the preparation method of the calcium carbonate coated by the dipeptide self-assembled fiber.
In a third aspect, the invention also provides application of the calcium carbonate coated by the dipeptide self-assembly fiber in preparation of a drug sustained-release carrier.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, a compound with specific properties is selected to modify the surface of calcium carbonate particles, and then the calcium carbonate is combined with dipeptide for adsorption, so that the calcium carbonate coated with the dipeptide self-assembly fibers is obtained, and has the characteristics of low toxicity and good drug slow-release effect; the calcium carbonate is nontoxic and can adapt to different human body environments, the specific dipeptide can improve the drug slow release effect, and the specific modified compound can be selected to tightly adsorb the dipeptide on the surface of the calcium carbonate, so that the drug slow release effect of the calcium carbonate coated by the dipeptide self-assembly fiber in a human body is obviously improved.
Drawings
FIG. 1 is the PDDA-CaCO in example 1 3 Scanning electron micrographs of the particles;
FIG. 2 is Fmoc-YL-NH in example 1 2 @PDDA-CaCO 3 Scanning electron micrographs of the particles;
FIG. 3 is the PSS-CaCO in example 2 3 Scanning electron micrographs of the particles;
FIG. 4 is Fmoc-YL-NH from example 3 2 @PDDA-CaCO 3 -scanning electron micrographs of SA particles;
FIG. 5 is Fmoc-YL-NH in example 4 2 @PDDA-CaCO 3 -scanning electron micrographs of DA particles.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
In the following examples, PDDA, PSS were purchased from Sigma-Aladdin.
Example 1
The embodiment provides a calcium carbonate particle coated by dipeptide self-assembly fiber, and the preparation method comprises the following steps:
(1) 0.1mL of PDDA was added to 10mL of water followed by 1M CaCl 2 The flask containing the above solution was placed on a magnetic stirrer and stirred at 1500rpm for 30 minutes, then 1mL Na was added 2 CO 3 Stirring the aqueous solution for 20 s, centrifuging the solution, washing the obtained particles with water and ethanol for three times, and vacuum drying to obtain particles marked as PDDA-CaCO 3 The scanning electron micrograph thereof is shown in FIG. 1.
(2) Fmoc-Y (120 mM) and L-NH 2 (480 mM) was dissolved in PBS buffer (pH =8,0.1mM, 2mL), and Fmoc Y and L-NH were allowed to react by adding 1mg of thermolysin 2 Carrying out condensation reaction for 2h to obtain Fmoc-YL-NH 2 。
(3) Fmoc-YL-NH obtained in the step (2) 2 (0.5 mg) was dissolved in an aqueous ethanol solution, sonicated, and then the particles obtained in step (1) were added to the above solution, adsorbed by shaking at 25 ℃, and finally the liquid was removed by rotary evaporation to obtain solid particles. Obtaining the calcium carbonate particles coated by the dipeptide self-assembly fibers, and the calcium carbonate particles are named as Fmoc-YL-NH 2 @PDDA-CaCO 3 The scanning electron micrograph is shown in FIG. 2.
Example 2
This example provides a calcium carbonate particle coated with dipeptide self-assembled fiber, and the preparation method is as follows:
(1) 0.02g PSS was added to 10mL water followed by 1M CaCl 2 The flask containing the above solution was placed on a magnetic stirrer and stirred at 1500rpm for 30 minutes, then 1mL Na was added 2 CO 3 The aqueous solution is continuously stirred for 20 seconds, finally the solution is taken out for centrifugation, the obtained particles are washed for three times by water and ethanol, and then the particles are dried in vacuum, and the obtained particles are marked as PSS-CaCO 3 The scanning electron micrograph is shown in FIG. 3
(2) Fmoc-Y (120 mM) and L-NH 2 (480 mM) was dissolved in PBS buffer (pH =8,0.1mM, 2mL), and Fmoc Y and L-NH were allowed to react by adding 1mg of thermolysin 2 Carrying out condensation reaction for 2h to obtain Fmoc-YL-NH 2 。
(3) Fmoc-YL-NH obtained in the step (2) 2 (0.5 mg) was dissolved in an aqueous ethanol solution, sonicated, and then the particles obtained in step (1) were added to the above solution, adsorbed by shaking at 25 ℃, and finally the liquid was removed by rotary evaporation to obtain solid particles. Obtaining the calcium carbonate particles coated by the dipeptide self-assembly fibers, and the calcium carbonate particles are named as Fmoc-YL-NH 2 @PSS-CaCO 3 。
Example 3
The embodiment provides a calcium carbonate particle coated by dipeptide self-assembly fiber, and the preparation method comprises the following steps:
(1) 0.1mL of PDDA was added to 10mL of water followed by 1M CaCl 2 The Erlenmeyer flask containing the above solution was placed on a magnetic stirrer and stirred at 1500rpm for 30 minutes, then 1mL of Na was added 2 CO 3 Stirring the aqueous solution for 20 s, centrifuging the solution, washing the obtained particles with water and ethanol for three times, and vacuum drying to obtain particles marked as PDDA-CaCO 3 。
(2) Fmoc-Y (120 mM) and L-NH 2 (480 mM) was dissolved in PBS buffer (pH =8,0.1mM, 2mL), and Fmoc Y and L-NH were allowed to react by adding 1mg of thermolysin 2 Carrying out condensation reaction for 2h to obtain Fmoc-YL-NH 2 。
(3) 0.2g of stearic acid was added to 20mL of an aqueous solution, the solution was dissolved by adjusting pH =9.8, and 80mg of PDDA-CaCO obtained in step (1) was added 3 Dispersing the granules in the solution, heating for 30min, washing the obtained granules with water and ethanol for 3 times, and vacuum drying to obtain granules marked as PDDA-CaCO 3 -SA。
(4) Fmoc-YL-NH obtained in the step (2) 2 (0.5 mg) was dissolved in an aqueous ethanol solution, sonicated, and then the particles obtained in step (3) were added to the above solution, adsorbed by shaking at 25 ℃, and finally the liquid was removed by rotary evaporation to obtain solid particles. Obtaining the calcium carbonate particles coated by the dipeptide self-assembly fibers, and the calcium carbonate particles are named as Fmoc-YL-NH 2 @PDDA-CaCO 3 SA, scanning electron micrograph of which is shown in FIG. 4.
Example 4
This example provides a calcium carbonate particle coated with dipeptide self-assembled fiber, and the preparation method is as follows:
(1) 0.1mL of PDDA was added to 10mL of water followed by 1M CaCl 2 The Erlenmeyer flask containing the above solution was placed on a magnetic stirrer and stirred at 1500rpm for 30 minutes, then 1mL of Na was added 2 CO 3 Stirring the aqueous solution for 20 s, taking out the solution, centrifuging, washing the obtained particles with water and ethanol for three times, and vacuum drying to obtain particles marked as PDDA-CaCO 3 。
(2) Fmoc-Y (120 mM) and L-NH 2 (480 mM) was dissolved in PBS buffer (pH =8,0.1mM, 2mL), and Fmoc Y and L-NH were allowed to react by adding 1mg of thermolysin 2 Carrying out condensation reaction for 2h to obtain Fmoc-YL-NH 2 。
(3) 2mg of dopamine hydrochloride and 0.3mL of CuSO are taken 4 /H 2 O 2 Adding the solution into Tris buffer solution with pH =8.5, 30mM and 5mL, stirring at 600rmp for 1.5 hr, centrifuging the solution, washing the obtained granules with water and ethanol for three times, and vacuum drying to obtain granules labeled as PDDA-CaCO 3 -DA。
(4) Fmoc-YL-NH obtained in the step (2) 2 (0.5 mg) was dissolved in an aqueous ethanol solution, sonicated, and then the particles obtained in step (3) were added to the above solution, adsorbed by shaking at 25 ℃, and finally the liquid was removed by rotary evaporation to obtain solid particles. Obtaining the calcium carbonate particles coated by the dipeptide self-assembly fibers, and the calcium carbonate particles are named as Fmoc-YL-NH 2 @PDDA-CaCO 3 -DA, the scanning electron micrograph of which is shown in FIG. 5.
Example 5
This example provides calcium carbonate particles prepared in accordance with example 1 except that Fmoc-Y was replaced with an equal amount of Fomc-T in step (2).
Example 6
This example provides calcium carbonate particles prepared in accordance with example 1 except that Fmoc-Y was replaced with an equal amount of Fomc-L in step (2).
Testing the slow release effect of the drug:
preparing 1mg/mL of Dox, dissolving in water for 2mL, adding 15mg of the calcium carbonate particles, co-culturing for 1h to ensure that the Dox is fully adsorbed on the surfaces of the particles, and finally carefully washing with water for three times for later use.
The pH of the cell environment was simulated to study the effect of different pH on drug release of calcium carbonate particles obtained after adsorption of the drug. 15mg of the calcium carbonate particles obtained after the adsorption of the drug were dispersed in 5ml of a buffer solution having ph =5.2, 6.5, and 7.4, respectively, and the reaction was carried out at 37 ℃. After 36h, 2.5mL of the supernatant was taken and analyzed for Dox release by measuring the absorbance at 559nm using a UV/Vis spectrometer. The liquid was removed and the same volume of fresh buffer was added to maintain the original volume.
The above tests were carried out on the products provided in examples 1, 5 and 6, with the following results:
the test result shows that the calcium carbonate coated by the dipeptide self-assembled fiber is tightly combined with the dipeptide through modifying the calcium carbonate, so that the drug slow-release effect of the calcium carbonate coated by the dipeptide self-assembled fiber is improved, and the toxicity is reduced; simultaneous selection of Fmoc-YL-NH 2 Compare Fmoc-LL-NH 2 And Fmoc-TL-NH 2 The sustained release effect can be further improved.
The applicant states that the present invention is illustrated by the above examples to the dipeptide self-assembled fiber coated calcium carbonate of the present invention and the preparation method and application thereof, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modifications of the present invention, equivalent substitutions of the raw materials of the product of the present invention, and the addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that, in the above embodiments, the various features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, the present invention does not separately describe various possible combinations.
Claims (11)
1. The preparation method of the calcium carbonate coated by the dipeptide self-assembled fiber is characterized by comprising the following steps:
(1) Mixing and dissolving calcium chloride and a charged compound, and then mixing the calcium chloride and a carbonate solution to obtain calcium carbonate particles with positive or negative charges on the surfaces;
(2) Mixing the calcium carbonate particles with positive charges or negative charges on the surface obtained in the step (1) with dipeptide to obtain calcium carbonate coated by the dipeptide self-assembly fiber;
the charged compound in the step (1) comprises any one of polydimethyldiallyl ammonium chloride, gelatin or polystyrene sulfonate;
the dipeptide in the step (2) is Fmoc-YL-NH 2 、Fmoc-LL-NH 2 Or Fmoc-TL-NH 2 Any one of them.
2. The method for preparing the dipeptide self-assembled fiber-coated calcium carbonate according to claim 1, wherein the mass ratio of the calcium chloride to the charged molecule in the step (1) is 1.
3. The method for preparing dipeptide self-assembled fiber-coated calcium carbonate according to claim 1, wherein the carbonate in step (1) comprises sodium carbonate and/or potassium carbonate.
4. The method for preparing calcium carbonate coated with dipeptide self-assembled fiber according to claim 1, wherein the dipeptide in step (2) is Fmoc-YL-NH 2 。
5. The method for preparing calcium carbonate coated with dipeptide self-assembled fiber according to claim 1, wherein Fmoc-YL-NH is added 2 The preparation method comprises the following steps: fmoc-Y, L-NH 2 Mixing with enzyme and dissolving to obtain Fmoc-YL-NH 2 。
6. The method for preparing the dipeptide self-assembled fiber-coated calcium carbonate according to claim 5, wherein the enzyme comprises thermolysin.
7. The method for preparing calcium carbonate coated with dipeptide self-assembled fibers according to claim 1, wherein step (2) further comprises step (2') of mixing the calcium carbonate particles having positive or negative charges on the surface obtained in step (1) with a hydrophilic compound or a hydrophobic compound before mixing the calcium carbonate particles having positive or negative charges on the surface obtained in step (1) with the dipeptide to obtain calcium carbonate particles having hydrophilic or hydrophobic groups on the surface.
8. The method for preparing dipeptide self-assembled fiber-coated calcium carbonate according to claim 7, wherein the hydrophilic compound of step (2') comprises dopamine.
9. The method for preparing dipeptide self-assembled fiber-coated calcium carbonate according to claim 7, wherein the hydrophobic compound of step (2') comprises stearic acid.
10. A dipeptide self assembled fiber coated calcium carbonate prepared by the method of preparing dipeptide self assembled fiber coated calcium carbonate according to any of claims 1-9.
11. Use of the dipeptide self-assembled fiber coated calcium carbonate according to claim 10 in the preparation of a drug sustained release carrier.
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