CN113009166B - Light-shading detection system and implementation method thereof - Google Patents
Light-shading detection system and implementation method thereof Download PDFInfo
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- CN113009166B CN113009166B CN202110195361.6A CN202110195361A CN113009166B CN 113009166 B CN113009166 B CN 113009166B CN 202110195361 A CN202110195361 A CN 202110195361A CN 113009166 B CN113009166 B CN 113009166B
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Abstract
The invention relates to the technical field of light-resistant detection of chemiluminescence immunoassay analyzers, and particularly discloses a light-resistant detection system, which comprises an outer light-resistant unit, an inner light-resistant unit, a reagent reaction unit, an optical detection unit and a movable light-resistant unit; the implementation method utilizes the units to carry out detection action; the detection action comprises preparation work in the early detection stage, reagent reaction process in the middle detection stage and photon reading of the detected object in the final detection stage. The invention is based on an external light-shading unit to block most external ambient light; an internal light-shading unit is used for blocking ambient light generated inside the light-shading detection system; in the middle-stage reagent reaction process of detection, a reagent reaction unit is used for allowing a substance or a mechanism to enter and exit the light-proof detection system, so that the reagent reaction process is promoted; in the final stage of reagent reaction, the light-resistant detection system is closed by moving the light-resistant unit to realize the isolation of external environment light; and the optical detection unit performs photon reading on the detected object at the end of detection to finish the detection process.
Description
Technical Field
The invention relates to the technical field of light-proof detection of a chemiluminescence immunoassay analyzer, in particular to a light-proof detection system and an implementation method thereof.
Background
In the current optical detection link in the test, a method of transferring a reaction tube loaded with a detected object to an optical detection part for photon reading is mainly adopted.
The design mode can be used for carrying out special light-shading design on the light detection component, the reaction tube is placed in the light detection component, and reliable photon reading is obtained through light detection through mechanical sealing.
During the reaction of the reagents, it is necessary to perform the transfer of the substance in the reaction tube, which is exposed to the external space; during the light detection, the reaction tube is transferred to the light detection part to block the ambient light. Such as: publication No.: CN110133314A, patent name: a full-automatic chemiluminescence immunoassay analyzer. The reaction and the light detection are in different environments, relatively complex reaction tube/cup transfer design and control are needed, and the volume and complexity of an instrument cannot be reduced in the link of transferring the reaction to the detection.
Disclosure of Invention
The invention aims to solve the technical problem of providing a light-shading detection system and an implementation method thereof, which unify the reaction and detection environment, ensure that the environment light is extremely low in the light detection link and cannot influence the light detection.
The technical problem to be solved by the invention is as follows:
on one hand, the light-resistant detection system comprises an outer light-resistant unit, an inner light-resistant unit, a movable light-resistant unit, a reagent reaction unit and a light detection unit;
the outer light-shading unit comprises a bottom plate, an outer cover which is arranged on the bottom plate and provided with a bin opening on the side surface, and a bin door which is matched with the bin opening; the inner light-shading unit, the reagent reaction unit and the light detection unit are arranged on the bottom plate and are positioned in the outer cover; the top of the outer cover is provided with a reaction inlet and a reaction outlet;
the movable light-shading unit is arranged at the reaction inlet and the reaction outlet;
the reagent reaction unit is connected with the inner side surface of the bin gate and does linear motion across the bin inlet or the bin outlet;
the inner light-shading unit is installed on one side, far away from the bin opening, of the bottom plate and is in sliding fit with the light detection unit.
The reagent strip is loaded on the reagent reaction unit; the bin door is electrically or manually closed, and is buckled on the bin opening of the outer cover and sealed. In the middle-stage detection reagent reaction process, the reagent reaction unit can realize the entrance and exit of substances or mechanisms into and out of the inner part of the outer cover through the reaction inlet and outlet; moving a light-shading unit to seal the reaction inlet and the reaction outlet at the end of reagent reaction to realize the obstruction of external ambient light; and the optical detection unit performs photon reading on the detected object at the end of detection to finish the detection process.
The invention is based on an external light-shading unit to block most external ambient light; an internal light-shading unit is used for blocking ambient light generated inside the light-shading detection system; the invention has simple structure and high integration level, does not need complex control design, and can be flexibly adjusted by the implementation method.
In some possible embodiments, to achieve photon readings for several fixed position detection objects or several movable detection objects; the light detection unit is arranged on the bottom plate in a sliding mode, and the sliding direction of the light detection unit is the same as the moving direction of the reagent reaction unit; the light detection unit comprises a photon counter, a photon counter motion mechanism used for controlling the photon counter to slide on the bottom plate, and a photon counter diaphragm which is arranged on a light sensing window of the photon counter and is in sliding fit with the inner light-shading unit.
In some possible embodiments, in order to protect the photon counter from ambient light during the light detection period, making the detection result inaccurate and even damaging, an internal light-shielding unit is provided; the inner light-shading unit comprises a photon counter light-shading mechanism and a hidden photoelectric switch triggering mechanism which are arranged on one side of the reagent reaction unit, which is far away from the bin opening; and the photon counter light-shading mechanism is in sliding fit with the photon counter diaphragm.
In some possible embodiments, in order to effectively realize the sliding fit of the light shielding mechanism of the photon counter and the photon counter; the light-shading mechanism of the photon counter is provided with a groove at one side close to the light detection unit, the diaphragm of the photon counter is provided with a circular boss structure at one side close to the groove, and the diaphragm of the photon counter is in sliding fit with the groove.
In some possible embodiments, a side of the hidden photoelectric switch triggering mechanism close to the light detection unit is provided with a photoelectric switch triggering rod; and the light detection unit is provided with a photoelectric switch trigger block matched with the photoelectric switch trigger rod for use.
The photoelectric switch trigger rod and the photoelectric switch trigger block are matched with each other to block light emitted by the photoelectric switch which plays a positioning/resetting role in a photon counter movement mechanism in the outer cover, so that the light cannot influence the environment light on the photon counter.
In some possible embodiments, to enable the opening or closing of the reaction ports; the movable light shading unit comprises a light shading plate fixing piece arranged on the outer cover and a light shading plate which is arranged on the light shading plate fixing piece in a sliding mode and used for opening or closing the detection opening; the light screen fixing piece is connected with the light screen in a sliding and sealing mode.
In some possible embodiments, in order to realize the connection between the light screen fixing piece and the light screen, the light leakage at the connection part is avoided; the light screen fixing piece is of a U-shaped structure, and a light screen channel is arranged on the inner side of the light screen fixing piece; the light screen is provided with a light screen boss which is matched with the opening for installation.
In some possible embodiments, a side of the light screen, which is away from the U-shaped structure, is provided with a light screen stop.
In some possible embodiments, the outer light-shielding unit further comprises a bin gate disposed corresponding to the bin opening, and the bin gate is connected to one end of the reagent reaction unit close to the bin opening;
the reagent reaction unit is a reagent strip loading device which is arranged in the outer light-resistant unit in a sliding mode and is connected with the bin gate, and the reagent strip loading device has temperature control capacity;
and a reagent reaction control device is also arranged on the outer side surface of the outer cover.
In some possible embodiments, in order to load reagent strips, the reagent strip loading device comprises two groups of loading mounting blocks which are arranged in parallel and one end of each loading mounting block is connected with a bin gate, and a plurality of reagent strip hole sites are arranged on each loading mounting block; and the plurality of reagent strip hole sites are arranged along the sliding direction of the loading mounting block.
On the other hand, the implementation method of the light-shielding detection system specifically comprises the following steps:
resetting related parts of the instrument; the method comprises resetting the relevant parts of the light-shielding detection system.
Preparation before testing: and replacing other disposable consumables used in the test. Placing the reagent strip on a reagent strip loading device, placing the sample in a specified position of an instrument, and closing a bin door and closing a reaction inlet and a reaction outlet;
and (3) testing: transferring the reactant/reagent to the next reaction/detection position by using a reagent reaction control device, controlling the temperature at the position of a reagent strip hole, and realizing the full reaction of the reagent and the sample after the set time is reached;
after the reaction is finished, closing the movable light-shading unit, and carrying out photon reading on the detected object by using a photon counter;
and (5) after the detection is finished, resetting related parts of the instrument.
Compared with the prior art, the invention has the beneficial effects that:
the invention is based on an external light-shading unit to block most external ambient light; an internal light-shading unit is used for blocking ambient light generated inside the light-shading detection system; in the middle-stage reagent reaction process of detection, the reagent reaction unit is applied, and a substance or a mechanism is allowed to enter and exit the light-shading detection system to promote the reagent reaction process; in the final stage of reagent reaction, the light-resistant detection system is closed by moving the light-resistant unit to realize the isolation of external environment light; the optical detection unit performs photon reading on the detected object at the final detection stage to complete the detection process;
the system has simple structure and high integration level, does not need complex control design, and can be flexibly adjusted by the implementation method.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a diagram of the position relationship between the base plate and the outer cover of the external light-shielding unit;
FIG. 3 is a schematic view of other components mounted on the base plate of the present invention;
FIG. 4 is a schematic structural diagram of a photon counter light shielding mechanism, a photon counter movement mechanism and a photon counter diaphragm in the invention;
FIG. 5 is a cross-sectional view of the photon counter light shielding mechanism, the photon counter, and the diaphragm of the photon counter;
FIG. 6 is a schematic diagram showing the correlation between the concealed photoelectric switch triggering mechanism and the photoelectric switch triggering block according to the present invention;
FIG. 7 is a schematic structural diagram of a mobile light-shielding unit according to the present invention;
FIG. 8 is a schematic diagram showing the structural relationship between the reagent reaction control device and the housing according to the present invention;
wherein: 11. a base plate; 111. mounting grooves; 12. a housing; 121. a bin mouth; 122. a reaction inlet and a reaction outlet; 13. a bin gate; 21. a photon counter light-shielding mechanism; 22. a hidden photoelectric switch trigger mechanism; 221. a photoelectric switch trigger lever; 31. a visor mount; 311. a visor channel; 32. a visor; 321. a visor boss; 322. a light screen stop; 41. a reagent strip loading device; 51. a photon counter; 52. a photon counter motion mechanism; 521. a photoelectric switch trigger block; 53. a photon counter diaphragm; 6. a reagent strip; 7. reagent reaction control device.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some, but not all, embodiments of the present application. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.
Reference herein to "first," "second," and similar words, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. Also, the use of the terms a, an, etc. do not denote a limitation of quantity, but rather denote the presence of at least one. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
In the implementation of the present application, "and/or" describes an association relationship of associated objects, which means that there may be three relationships, for example, a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone.
In the description of the embodiments of the present application, the meaning of "a number" means one or more unless otherwise specified. For example, if a reference post is one or more than one reference post.
The invention is further described below with reference to the following figures and examples.
The invention is realized by the following technical scheme, as shown in figures 1-8,
a light-resistant detection system comprises an outer light-resistant unit, an inner light-resistant unit, a movable light-resistant unit, a reagent reaction unit and a light detection unit;
the outer light-shading unit comprises a bottom plate 11, an outer cover 12 which is arranged on the bottom plate 11 and provided with a bin opening 121 on the side surface, and a bin door 13 which is matched with the bin opening 121; the internal light-shading unit, the reagent reaction unit and the light detection unit are arranged on the bottom plate 11 and are positioned in the outer cover 12; the top of the housing 12 is provided with a reaction inlet/outlet 122;
the mobile light-shielding unit is arranged at the reaction inlet and outlet 122;
the reagent reaction unit is connected with the inner side surface of the bin gate 13 and does linear motion spanning into or across the bin outlet 121;
the inner light-avoiding unit is arranged on one side of the bottom plate 11 far away from the bin opening 121 and is in sliding fit with the light detection unit.
Preferably, the outer cover 12 is box-shaped, and forms a light-resistant detection system installation inner cavity with the bottom plate 11, and the inner light-resistant unit, the movable light-resistant unit, the reagent reaction unit and the light detection unit are installed in the inner cavity;
the bottom of the outer cover 12 is arranged on the bottom plate 11, the bin port 121 is arranged on the side surface of the outer cover, and the reaction inlet and outlet 122 is arranged on the top surface of the outer cover;
preferably, as shown in fig. 1 and 2, the housing 12 has a square structure.
Preferably, the bottom plate 11 is provided with a mounting groove 111, and the bottom of the reagent reaction unit is provided with a slide rail matched with the mounting groove 111; the length direction of the mounting groove 111 is perpendicular to the side of the door 13.
The outer light-resistant unit is a platform for mounting and bearing all parts of the light-resistant detection system and a main barrier for preventing external environment light from entering the light-resistant detection system.
The inner light-avoiding unit is an equipment assembly for blocking self-luminescence inside the cavity of the light-avoiding detection system and enhancing the light-avoiding capability of the photon counter 51.
And the movable light-shading unit seals the detection operation inlet and outlet before performing light detection.
The reagent reaction unit supports the conditions required during the reagent reaction.
The light detection unit performs photon reading on the detected object during light detection.
According to the invention, the bin gate 13 is connected with the reagent reaction unit, when the reagent strip 6 needs to be loaded on the reagent reaction unit, the bin gate 13 is controlled to move towards the outer side far away from the bin opening 121, the reagent reaction unit is in sliding fit with the bottom plate 11, so that the reagent reaction unit is driven to move towards one side of the bin opening 121, and the reagent reaction unit is pulled out, so that the loading of the reagent strip 6 can be realized;
the reagent reaction unit and the door 13 form a drawer structure.
Preferably, when the bin door 13 and the bin opening 121 are closed, the bin door 13 is attached to the bin opening 121;
in order to make the two close to each other when the door is closed, rubber, foam or other flexible materials are arranged on the side where the door 13 and the bin opening 121 are close to each other;
in order to effectively block the light from entering the light-shading detection system from the joint of the bin door 13 and the bin opening 121; the bin gate 13 is engaged with the bin opening 121.
When in use, the bin door 13 slides outwards electrically or manually to drive the reagent reaction unit to move out of the bin opening 121, and the reagent strip 6 is loaded on the reagent reaction unit; the door 13 is closed electrically or manually, and is fastened to and closed by the door opening 121 of the housing 12. In the middle-stage reagent reaction process of detection, the reagent reaction unit can realize the entrance and exit of substances or mechanisms into and out of the inner part of the outer cover 12 through the reaction inlet and outlet 122; before the light detection, the light-shading unit is moved to seal the reaction inlet and outlet 122, so that external ambient light is blocked; and the optical detection unit performs photon reading on the detected object at the end of detection to finish the detection process.
The test strip 6 described herein may be a sample carrier or a reagent carrier.
The invention is based on an external light-shading unit to block most external ambient light; an internal light-shading unit is used for blocking ambient light generated inside the light-shading detection system; the invention has simple structure and high integration level, does not need complex control design, and can be flexibly adjusted by the implementation method.
In some possible embodiments, to achieve photon readings for several fixed position detection objects or several movable detection objects; the bottom of the light detection unit is provided with a slide rail matched with the mounting groove 111, and the sliding direction of the slide rail is the same as the moving direction of the reagent reaction unit; the light detection unit comprises a photon counter 51, a photon counter motion mechanism 52 for controlling the photon counter 51 to slide on the bottom plate 11, and a photon counter diaphragm 53 which is arranged on a light-sensitive window of the photon counter 51 and is in sliding fit with the internal light-resistant unit.
The photon counter 51 is fixed on a photon counter moving mechanism 52, and the photon counter moving mechanism 52 is used for controlling the photon counter 51 to slide along the long direction of the installation groove 111, so as to bring the photon counter 51 to a reset position and each light detection position.
The diaphragm 53 of the photon counter is fixed on the light-sensitive window of the photon counter 51, so that stray light can enter the light-sensitive window of the photon counter 51 as little as possible during light detection, and the photon reading is reliable; the photon counter moving mechanism 52 moves to the reset position, and the ambient light entering the light sensing window of the photon counter 51 is greatly weakened by matching with the internal light-shading unit.
In some possible embodiments, as shown in fig. 3, in order to protect the photon counter 51 from ambient light during light detection, making its detection result inaccurate or even damaging; the inner light-avoiding unit comprises a photon counter light-avoiding mechanism 21 and a hidden photoelectric switch triggering mechanism 22 which are arranged on one side of the reagent reaction unit, which is far away from the bin opening 121; the photon counter light-shielding mechanism 21 is in sliding fit with the photon counter diaphragm 53.
The light shielding mechanism 21 of the photon counter is not limited to a shutter structure electrically controlled and installed on the light sensing window of the photon counter 51, and may be a mechanical light shielding chamber structure when the photon counter 51 moves to a fixed position. The former can realize the controlled light-shielding at any time and place; the latter can be used in the motion mode of the photon counter 51, can realize light shielding at a fixed position, and has relatively simple design.
In some possible embodiments, as shown in fig. 4 and 5, in order to effectively realize the sliding fit of the photon counter light shielding mechanism 21 and the photon counter 51; one side of the photon counter light-shading mechanism 21 close to the light detection unit is provided with a groove, one side of the photon counter diaphragm 53 close to the groove is provided with a circular boss structure, and the photon counter diaphragm 53 is in sliding fit with the groove.
Preferably, the longitudinal axis of the groove is disposed in parallel with the longitudinal axis of the mounting groove 111.
The length direction of the slide rail is consistent with the sliding direction of the reagent reaction unit on the bottom plate 11, and the detection of the light detection unit at different positions can be realized through sliding fit.
Preferably, as shown in fig. 4, a photon counter 51 is mounted on the photon counter moving mechanism 52, and when the photon counter moving mechanism moves to the reset position, a photon counter diaphragm 53 mounted on a light-sensitive window of the photon counter 51 enters the photon counter light-shielding mechanism 21;
as shown in fig. 5, one side of the aperture 53 of the photon counter, which is close to the groove, is provided with an annular boss, and light can enter the light sensing window of the photon counter 51 only through the center of the annular boss; when the photon counter movement mechanism 52 is reset, the circular boss enters the groove, and the distance between the circular boss and the groove is small. The materials of the two are blackened, so that light rays are prevented from entering a light sensing window of the photon counter 51, and the purpose of avoiding light of the photon counter 51 is achieved.
Preferably, a shutter is arranged on the photon counter diaphragm 53, and when light detection is needed, the shutter is opened; the shutter is closed at the rest time, and the requirement that the photon counter 51 is shielded from light at any position at any time can be realized.
In some possible embodiments, as shown in fig. 6, a photoelectric switch trigger rod 221 is disposed on one side of the hidden photoelectric switch trigger mechanism 22 close to the light detection unit; the light detection unit is provided with a photoelectric switch trigger block 521 used in cooperation with the photoelectric switch trigger lever 221.
The photoelectric switch trigger block 521 is installed on one side of the photon counter motion mechanism close to the photoelectric switch trigger rod 221.
The photoelectric switch trigger rod 221 and the photoelectric switch trigger block 521 cooperate with each other to block light emitted from the photoelectric switch that has a positioning/resetting function on the photon counter movement mechanism 52 in the housing 12, so that the light does not affect the photon counter 51 by ambient light.
The photon counter movement mechanism 52 moves to the reset position as determined by the concealed opto-electronic switch triggering mechanism 22; when the moving mechanism 52 of the photon counter moves to the reset direction, the triggering block 521 of the photoelectric switch contacts the triggering rod 221 of the photoelectric switch and presses the latter into the hidden triggering mechanism 22 of the photoelectric switch.
The reset photoelectric switch is arranged in the hidden photoelectric switch trigger mechanism 22, the photoelectric switch trigger rod 221 enters a photosensitive area of the reset switch to generate a reset photoelectric signal, the motion of the motion mechanism 52 of the photon counter stops, and the reset action of the motion mechanism 52 of the photon counter is realized. The self-luminescence generated by the reset photoelectric switch is blocked by the internal structure and the spacer ring/baffle plate inside the hidden photoelectric switch triggering mechanism 22 and can not be emitted to the inner cavity of the light-resistant detection system. When the photon counter moving mechanism 52 moves outwards, the photoelectric switch trigger rod 221 automatically pops up, and the reset photoelectric signal disappears.
Preferably, the photon counter moving mechanism 52 is mainly used for controlling the light detection unit to move along the length direction of the installation groove 111, and may be a screw driving device or a linear push rod.
In some possible embodiments, to enable the opening or closing of the reaction access 122; as shown in fig. 1 and 7, the mobile light shielding unit includes a light shielding plate holder 31 mounted on the housing 12, a light shielding plate 32 slidably mounted on the light shielding plate holder 31 and used for opening or closing the detection opening; the light shielding plate fixing member 31 is connected with the light shielding plate 32 in a sliding and sealing manner.
In some possible embodiments, as shown in fig. 7, in order to realize the connection between the light shielding plate fixing member 31 and the light shielding plate 32, light leakage at the connection position is avoided; the light screen fixing piece 31 is of a U-shaped structure, and a light screen channel 311 is arranged on the inner side of the U-shaped structure; the light screen 32 is provided with a light screen boss 321 fitted into the light screen channel 311.
The light shielding plate channel 311 is of a U-shaped structure and is provided with an opening.
The visor mount 31 is bolted to the housing 12.
Preferably, a flexible material such as a rubber pad and a sealing strip may be padded between the light shielding plate fixing member 31 and the outer cover 12 to isolate external ambient light from entering the interior of the light-shielding detection system through a gap between the light shielding plate fixing member 31 and the outer cover 12.
Preferably, a light shielding plate blocking head 322 is arranged on one side of the light shielding plate 32 far away from the U-shaped structure.
When in use, the light screen 32 moves from the opening side of the U-shaped structure to the bottom side thereof, so as to close the reaction inlet/outlet 122, and conversely, open the reaction inlet/outlet;
preferably, the depth and width of the recess of the visor channel 311 match the visor projection 321; the light screen bosses 321 are arranged on three surfaces of the light screen 32, and when the light screen 32 is inserted into the light screen fixing piece 31 to the bottom, the light screen channel 311 is exactly matched with the light screen bosses 321 on the three surfaces; the opening end of the light shielding plate fixing member 31 is attached to the light shielding plate stopper 322. Light cannot enter the housing 12 through the cavity of the mask mount 31.
Preferably, the light shielding plate 32 is provided with an installation positioning hole; a light screen control arm is arranged on the installation positioning hole to shield and expose the light screen fixing piece 31 along the light screen channel 311, so that in the reagent reaction period, substances or mechanisms can enter and exit the light-shading detection system through the reaction inlet and outlet 122 to promote the reagent reaction process; in the final stage of reagent reaction, the light shielding plate 32 is controlled to close the light shielding detection system, so that the purpose of blocking external ambient light is achieved.
Preferably, the shutter plate control arm is disposed on the top surface of the shutter plate 32.
Preferably, the light screen fixing piece 31 and the light screen boss 321 are separated by a wear-resistant material, which is beneficial to reducing friction between the light screen fixing piece and the light screen boss and improving sliding smoothness.
In some possible embodiments, as shown in fig. 3, the reagent reaction unit is a reagent strip loading device 41 slidably mounted on the bottom plate 11 and connected to the door 13;
the reagent reaction unit is mainly used for loading reagent strips 6 and controlling the temperature in the reaction process;
the reagent strip 6 is loaded with a sample and various reagents;
the reagent strip loading device 41 is provided with a reagent strip temperature control assembly which can carry out independent temperature control on the two reagent strip loading devices 41, thereby indirectly carrying out temperature control on the liquid in the reagent strip 6 and keeping the liquid in a temperature range specified by a reagent system; to meet the requirements of the reagent reaction process.
As shown in fig. 8, it is preferable that a reagent reaction control device 7 is further included, and the reagent reaction control device 7 is disposed on the top of the housing 12, and can perform a function of extracting the reactant/reactant to the next reaction/detection position.
During the reagent reaction, the inner cavity of the light-shielding detection system is communicated with the outside, and the reagent reaction control device 7 enters the outer cover 12 through the reaction inlet and outlet 122, so that various functions can be realized, such as moving the reagent to the next reaction/detection position to adapt to the requirements of a reagent system and flexibly realize the test process.
After the reagent reaction process is finished, the reagent reaction control device 7 exits from the interior of the outer cover 12, and the light shielding plate 32 shields the reaction inlet and outlet 122, so that photon reading can be performed.
Preferably, the reagent reaction control device 7 is a magnetic suction device or a liquid suction needle; when the reaction device is magnetic equipment, the magnetic equipment is moved to the next reaction position through magnetic beads for absorbing reactants, and the next reaction is carried out; the magnetic attraction device can be a magnetic bar.
When the reaction vessel is a liquid suction needle, the reaction product is sucked by the liquid suction needle, and then the liquid suction needle is moved to the next reaction position to carry out the next reaction.
In some possible embodiments, as shown in fig. 3, in order to load the reagent strips 6, the reagent strip loading device 41 includes two sets of loading mounting blocks arranged in parallel and having one end connected to the door 13, and a plurality of reagent strip 6 holes are arranged on the loading mounting blocks to be suitable for an application scenario where one or more reagent strips 6 are loaded; and a plurality of reagent strip 6 hole sites are arranged along the sliding direction of the loading and mounting block.
The first end and the last end of the reagent strip 6 are respectively loaded on the two groups of loading and installing blocks, and the two groups of loading and installing blocks provide stable reaction and detection loading positions for the reagent strip 6;
preferably, the middle of the loading mounting block at the end of the reagent strip 6 is provided with a hole or slot to provide a detection window for light detection. Of course, a special central opening may not be provided depending on the installation direction and position of the photon counter 51.
An implementation method of a light-shielding detection system specifically comprises the following steps:
and resetting related parts of the instrument, including resetting related parts of the light-shielding detection system.
Preparation before testing: and replacing other disposable consumables used in the test. Placing the reagent strip 6 on the reagent strip loading device 41, placing the sample in the specified position of the instrument, closing the door 13 and closing the reaction inlet/outlet 122; the method specifically comprises the following steps:
the bin gate 13 is pulled out or automatically popped up, the reagent strip 6 is manually loaded on the reagent strip loading device 41, and the sample is placed in the designated position of the instrument; the door 13 is closed and the hatch 121 of the system housing 12 is closed.
And (3) carrying out a testing link: transferring the reactant/reagent to the next reaction/detection position by using a reagent reaction control device 7, controlling the temperature at the hole position of the reagent strip 6, and realizing the full reaction of the reagent and the sample after the set time is reached;
after the reaction is completed, the movable light-shielding unit is closed, and the photon counter 51 performs photon reading on the object to be detected. The method specifically comprises the following steps:
the reagent reaction control device 7 exits the inner part of the outer cover 12, and the reaction inlet and outlet 122 is closed; the photon counter movement mechanism 52 moves to a first light sensing position and the photon counter 51 takes a photon reading; the next light sensing position movement and photon readings are performed as required until photon readings are completed for all positions.
And (5) after the detection is finished, resetting related parts of the instrument.
In conclusion, the invention unifies the reaction and detection environments, ensures that the light detection is not influenced by extremely low ambient light in the light detection link. Therefore, the instrument can be greatly simplified in structure and design in the detection process, and the volume and weight of the instrument can be fundamentally reduced.
The above embodiments are described in detail, and specific examples are applied in the present application to explain the principles and embodiments of the present application, and the description of the above embodiments is only used to help understand the method and the core idea of the present application; meanwhile, for a person skilled in the art, according to the idea of the present application, the specific implementation manner and the application scope may be changed, and in summary, the content of the present specification should not be construed as a limitation to the present application.
Claims (6)
1. A light-resistant detection system, its characterized in that: comprises an outer light-proof unit, an inner light-proof unit, a movable light-proof unit, a reagent reaction unit and an optical detection unit;
the inner light-resistant unit, the reagent reaction unit and the light detection unit are arranged in the outer light-resistant unit;
the outer light-resistant unit comprises an outer cover, the side surface of the outer light-resistant unit is provided with a bin opening, the top surface of the outer light-resistant unit is provided with a reaction inlet and a reaction outlet, and the reagent reaction unit is slidably mounted in the outer light-resistant unit and does linear motion crossing the bin opening;
the inner light-shading unit is arranged on one side of the reagent reaction unit, which is far away from the bin opening;
the light detection unit is in sliding fit with the inner shading unit and slides along the sliding direction of the reagent reaction unit;
the movable light-shading unit is arranged on the reaction inlet and outlet and is used for opening or closing the reaction inlet and outlet;
the light detection unit comprises a photon counter, a photon counter motion mechanism for controlling the photon counter to slide, and a photon counter diaphragm which is arranged on a light-sensitive window of the photon counter and is in sliding fit with the inner light-shading unit;
the inner light-shading unit comprises a photon counter light-shading mechanism and a hidden photoelectric switch triggering mechanism which are arranged on one side of the reagent reaction unit, which is far away from the bin opening; the photon counter light-shading mechanism is in sliding fit with a photon counter diaphragm;
a groove is formed in one side, close to the light detection unit, of the photon counter light-shading mechanism, an annular boss structure is arranged on one side, close to the groove, of the photon counter diaphragm, and the photon counter diaphragm is in sliding fit with the groove;
a photoelectric switch trigger rod is arranged on one side, close to the light detection unit, of the hidden photoelectric switch trigger mechanism; and the light detection unit is provided with a photoelectric switch trigger block matched with the photoelectric switch trigger rod for use.
2. A light-shielding detection system according to claim 1, wherein: the outer shading unit also comprises a bottom plate, and the outer cover is arranged on the bottom plate; the movable light shading unit comprises a light shading plate fixing piece arranged on the outer cover and a light shading plate which is arranged on the light shading plate fixing piece in a sliding mode and used for opening or closing the detection opening; the light screen fixing piece is connected with the light screen in a sliding and sealing mode.
3. A light-resistant detection system according to claim 2, wherein: the light screen fixing piece is of a U-shaped structure, and a light screen channel is arranged on the inner side of the light screen fixing piece; the light screen is provided with a light screen boss which is matched with the opening for installation.
4. A light-tight detection system according to any one of claims 1-3, wherein: the outer light-shading unit also comprises a bin door arranged corresponding to the bin opening, and the bin door is connected with one end, close to the bin opening, of the reagent reaction unit;
the reagent reaction unit is a reagent strip loading device which is arranged in the outer light-shading unit in a sliding manner and is connected with the bin gate, and the reagent strip loading device has temperature control capability;
and a reagent reaction control device is also arranged on the outer side surface of the outer cover.
5. A light-tight detection system according to claim 4, wherein: the reagent strip loading device comprises two groups of loading mounting blocks which are arranged in parallel and one end of each loading mounting block is connected with the bin gate, and a plurality of reagent strip hole sites are arranged on the loading mounting blocks; and a plurality of reagent strip hole sites are arranged along the sliding direction of the loading mounting block.
6. A method of implementing a light-tight detection system according to any one of claims 1 to 5, wherein: the method specifically comprises the following steps:
resetting related components of the instrument, including resetting related components of the light-shielding detection system;
replacing other disposable consumables used in the test;
placing the reagent strip on a reagent strip loading device, placing the sample in a specified position of an instrument, and closing a bin door and a closed reaction inlet and outlet;
carrying out a testing link; transferring the reactant/reagent to the next reaction/detection position by using a reagent reaction control device, controlling the temperature at the position of a reagent strip hole, and realizing the full reaction of the reagent and the sample after the set time is reached;
after the reaction is finished, closing the movable light-shading unit, and carrying out photon reading on the detected object by using a photon counter;
and after the detection is finished, resetting related parts of the instrument, including resetting related parts of the light-shielding detection system.
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| CN202110195361.6A CN113009166B (en) | 2021-02-20 | 2021-02-20 | Light-shading detection system and implementation method thereof |
| PCT/CN2021/087664 WO2022174512A1 (en) | 2021-02-20 | 2021-04-16 | Light-shielding detection system and implementation method thereof |
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| CN202110195361.6A CN113009166B (en) | 2021-02-20 | 2021-02-20 | Light-shading detection system and implementation method thereof |
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| CN113009166A (en) | 2021-06-22 |
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