CN113009125B - Breath metabolism measurement system - Google Patents
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- CN113009125B CN113009125B CN202110213588.9A CN202110213588A CN113009125B CN 113009125 B CN113009125 B CN 113009125B CN 202110213588 A CN202110213588 A CN 202110213588A CN 113009125 B CN113009125 B CN 113009125B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B2010/0083—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements for taking gas samples
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Abstract
The present disclosure provides an expiratory metabolism assay system, comprising: the device comprises an organic vapor micro-injection module, an air suction pump, a monomer breath print sampling module, an aerosol nozzle, a gas electromagnetic valve, a zero-level air purifier and an automatic solvent desorption module; the first end of the air pump is connected with an emergent port of the organic vapor micro-injection module, and the second end of the air pump is connected with the first end of the liner tube; the gas outlet of the monomer breath print sampling module is connected with the second end of the liner tube, and a stirrer adsorption extraction rod is arranged in the liner tube to sample the breath prints of the experimental animals; the air outlet of the monomer breath print sampling module is connected with the nozzle interface of the aerosol nozzle, and the air inlet of the monomer breath print sampling module is connected with the air outlet of the zero-level air purifier; the gas electromagnetic valve controls zero-order air to enter the monomer breath print sampling module and synchronously blocks inhaled exposed substances from entering the monomer breath print sampling module; the automatic solvent desorption module is used for detecting the breath metabolites collected by the stirrer adsorption extraction rod.
Description
Technical Field
The disclosure relates to the field of biological experiments, in particular to an expiratory metabolism determination system.
Background
In traditional expiratory metabolome and other metabolome studies, detection of time point metabolism after inhalation exposure has been achieved, but currently there is no technology for the real-time combination of inhalation exposure and expiratory blotting.
In studies in the fields of biology, medicine, pharmacy, etc., the real-time response of expiratory metabolome changes to inhalation exposure has an irreplaceable role in revealing the immediate health effects of foreign substances entering the organism via the inhalation route.
Accordingly, there is a need to provide an expiratory metabolic assay system that can combine natural inhalation exposure with an expiratory blot technique in real time.
Disclosure of Invention
Technical problem to be solved
The present disclosure provides an expiratory metabolic assay system to solve the above-presented technical problems.
(II) technical scheme
According to an aspect of the present disclosure, there is provided an expiratory metabolic assay system comprising:
the organic vapor micro-injection module outputs organic vapor from an outlet;
the first end of the air pump is connected with the emergent port of the organic vapor micro-injection module; the second end of the air pump is connected with the first end of the liner tube;
the device comprises a single breath print sampling module, an experimental animal is placed in the single breath print sampling module, an air outlet of the single breath print sampling module is connected with the second end of a liner tube, a stirrer adsorption extraction bar is arranged in the liner tube, and breath metabolites of the experimental animal are sampled;
the aerosol nozzle is radially provided with at least one nozzle interface; the nozzle interface is connected with an air outlet of the single breath print sampling module;
the gas electromagnetic valve is connected with the first end of the aerosol nozzle;
the gas outlet of the zero-order air purifier is connected with the gas inlet interface of the gas electromagnetic valve and/or the gas inlet of the monomer breath print sampling module; the gas electromagnetic valve controls zero-level air output by the zero-level air purifier to enter the monomer breath print sampling module and synchronously blocks inhaled exposed substances from entering the monomer breath print sampling module; and
and the automatic solvent desorption module is used for detecting the breath metabolites collected by the stirrer adsorption extraction rod.
In some embodiments of the present disclosure, further comprising: the external standard module is connected with the second end of the liner tube and is used for simulating the expiration process of the experimental animal and external standard method quantification of target metabolites; and an organic steam interface of the external standard module is connected with an air outlet of the zero-order air purifier.
In some embodiments of the present disclosure, further comprising: an air background module coupled to the second end of the liner, the air background module configured to detect an air background in the expiratory metabolic assay system.
In some embodiments of the present disclosure, the monomeric breath print sampling module comprises:
the section of the mask is of a double-layer cavity tube structure;
an air inlet communicated with the first end of the inner cavity tube of the face mask;
the air outlet is communicated with the outer wall surface of the outer cavity tube of the mask;
the first end of the experimental animal oral-nasal interface is connected with the second end of the mask; the head of the experimental animal extends into the second end of the oral-nasal interface of the experimental animal and is opposite to the second end of the inner cavity tube of the face mask;
the first end of the main pipe is connected with the second end of the face mask in a splicing and inserting manner; and
moisture absorption strip shaped plate, moisture absorption strip shaped plate first end with be responsible for first end and piece together to insert the connection, moisture absorption strip shaped plate second end with be responsible for the second end and pass through limit baffle and connect, moisture absorption strip shaped plate with laboratory animal's ventral surface is relative.
In some embodiments of the present disclosure, the main tube and the moisture-absorbing strip-shaped plate are transparent structures, and the single breath blot sampling module further comprises:
the camera fixing ring is sleeved on the outer wall of the main pipe;
the camera is connected with the camera fixing ring; the lens of the camera is opposite to the ventral surface of the experimental animal; and
and the breath video collector is used for receiving the breath video of the experimental animal shot by the camera.
In some embodiments of the present disclosure, further comprising: a first end of the multifunctional ejector is connected with a second end of the aerosol nozzle, and the second end of the multifunctional ejector is connected with an air outlet of the zero-level air purifier; the multifunctional ejector comprises:
the ejector cavity is connected with the first end of the gas-liquid interface;
the second end of the gas-liquid interface is respectively connected with the machine steam micro-injection module;
a first end of the two-phase nozzle extends into the ejector chamber, a second end of the two-phase nozzle is connected with an air outlet of the zero-level air purifier, and the two-phase nozzle carries out pneumatic atomization on the liquid in the ejector chamber and generates aerosol; and
the turbulent cone is arranged in the ejector cavity, is opposite to the first end of the two-phase nozzle and is used for impacting the aerosol in the ejector cavity.
In some embodiments of the present disclosure, the organic vapor microinjection module comprises:
an injector, wherein the cavity of the injector is filled with organic vapor;
the injector is inserted into the groove body of the constant-temperature groove seat;
the injection pushing rod is fixed on the thermostatic bath seat;
the stress plate is sleeved on the guide rod; and
and the driving device drives the stress plate to move towards the constant-temperature groove seat along the guide rod, and the stress plate presses the injector injection rod to enable the organic steam to be output through the injector injection port.
In some embodiments of the present disclosure, further comprising: the breath print operating cabin is used for accommodating the air pump, the single breath print sampling module, the aerosol nozzle and the gas electromagnetic valve; the breath print operating compartment comprises:
the air suction pump penetrates through the top cover and is connected with the emergent port of the organic vapor micro-injection module;
the second end of the aerosol nozzle penetrates through the bottom cover; the top cover and the bottom cover are both internally embedded with an inner track and an outer track;
the two curved surface sliding doors are arranged between the top cover and the bottom cover and respectively slide along the inner track and the outer track through rollers;
the two ends of the support frame are respectively connected with the top cover and the bottom cover; and
the supporting disk, the supporting disk sets up between top cap and the bottom, just the supporting disk wears to establish on the support frame.
In some embodiments of the present disclosure, the automatic solvent desorption module comprises:
the accommodating cavity is provided with a plurality of accommodating holes,
the transmission rubber rod is arranged between the two smooth electromagnetic rods, and two ends of the smooth electromagnetic rods and two ends of the transmission rubber rod are respectively arranged in the accommodating cavity in a penetrating manner;
the sample bottle is horizontally arranged between the two smooth electromagnetic rods; a solvent and the stirrer adsorption extraction rod are arranged in the sample bottle, and the stirrer adsorption extraction rod is provided with a magnetic core;
the stepping motor is used for electromagnetically driving the smooth electromagnetic rod to rotate; and
and the desorption process controller controls the rotation direction, the rotation speed and the rotation time of the sample bottle.
In some embodiments of the present disclosure, the automatic solvent desorption module further comprises: a sample bottle magnetic pipe frame, wherein the sample bottle after desorption is vertically inserted into the sample bottle magnetic pipe frame; and magnetic fields with opposite magnetic field directions are arranged at two ends of the magnetic pipe frame of each sample bottle, so that the stirrer adsorption extraction rod in each sample bottle is attached to the inner wall of the sample bottle.
(III) advantageous effects
According to the technical scheme, the breath metabolism measuring system disclosed by the invention has at least one or part of the following beneficial effects:
(1) the breath metabolism determination system provided by the disclosure can realize synchronous operation of inhalation exposure and breath blotting on experimental animals.
(2) The external standard module can simulate the exhalation process of the experimental animal and the external standard method quantification of the target metabolite, and is beneficial to the targeted research.
(3) The air background module is arranged in the breathing metabolic system, so that the air background in the breathing metabolic system can be detected, and the influence of the air background on the breathing metabolic system is researched.
(4) The moisture absorption strip-shaped plate in the single breath blot sampling module in the disclosure enables metabolites in exhaled air of experimental animals entering carrier gas to be free from interference of discharged substances, namely excrement and urine.
(5) The open transparent design of the chest and abdomen area of the experimental animal in the single breath print sampling module in the disclosure is combined with breath video acquisition, so that a relatively friendly breath physiological monitoring environment which is not closed to the experimental animal is provided.
(6) The combined use of the multifunctional ejector and the organic vapor microinjection module in the present disclosure supports the inhalation exposure operation of mixed gas, liquid aerosol and organic vapor to experimental animals.
(7) The breath print operation cabin can be opened at any angle, and the experiment operation is more convenient.
(8) The automatic solvent desorption module in the disclosure keeps the solvent desorption operation process consistent, ensures relatively stable desorption efficiency, and provides convenience for the pretreatment of samples during off-line detection.
Drawings
FIG. 1 is a schematic view of an expiratory metabolism assay system according to an embodiment of the disclosure.
FIG. 2 is another perspective view of an expiratory metabolic determination system according to an embodiment of the disclosure.
Fig. 3 is a schematic front view of an expiratory metabolic determination system according to an embodiment of the disclosure.
FIG. 4 is a schematic side view of an expiratory metabolic determination system according to an embodiment of the disclosure.
Fig. 5 is a disassembled view of the structure of fig. 1.
Fig. 6 is a disassembled view of the structure of fig. 2.
Fig. 7 is a disassembled view of the structure of fig. 3.
Fig. 8 is a disassembled view of the structure of fig. 4.
Fig. 9 is a partial enlarged view of the automatic solvent desorption module of fig. 1.
Fig. 10 is a schematic structural diagram of a single breath print sampling module.
Fig. 11 is a schematic sectional structure diagram of a single breath print sampling module.
Fig. 12 is a schematic structural diagram of an external standard module.
Fig. 13 is a schematic structural view of the gas solenoid valve.
Fig. 14 is a schematic structural view of the multifunctional ejector.
Detailed Description
The present disclosure provides an expiratory metabolism assay system, comprising: the device comprises an organic vapor micro-injection module, an air suction pump, a monomer breath print sampling module, an aerosol nozzle, a gas electromagnetic valve, a zero-level air purifier and an automatic solvent desorption module; the first end of the air pump is connected with an emergent port of the organic vapor micro-injection module, and the second end of the air pump is connected with the first end of the liner tube; the gas outlet of the monomer breath print sampling module is connected with the second end of the liner tube, and a stirrer adsorption extraction rod is arranged in the liner tube to sample breath metabolites of the experimental animal; the air outlet of the monomer breath print sampling module is connected with the nozzle interface of the aerosol nozzle, and the air inlet of the monomer breath print sampling module is connected with the air outlet of the zero-level air purifier; the gas electromagnetic valve controls zero-order air to enter the monomer breath print sampling module and synchronously blocks inhaled exposed substances from entering the monomer breath print sampling module; the automatic solvent desorption module is used for detecting the breath metabolites collected by the stirrer adsorption extraction rod. The present disclosure enables the synchronous operation of inhalation exposure and breath blotting of experimental animals.
For the purpose of promoting a better understanding of the objects, aspects and advantages of the present disclosure, reference is made to the following detailed description taken in conjunction with the accompanying drawings.
Certain embodiments of the present disclosure will now be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the disclosure are shown. Indeed, various embodiments of the disclosure may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements.
Before describing a solution to the problem, it is helpful to define some specific vocabulary.
As used herein, "inhalation exposure" refers to a process in which a substance, which is an inhalation exposure pathway, naturally or artificially forms an aerosol and is infused into a living body through the respiratory tract during the natural respiration of the living body.
As used herein, the term "breath print" refers to the collection and detection of metabolites (e.g., volatile organic compounds) contained in exhaled breath sols, i.e., the sampling and detection process in the breath metabolome study. The research object of metabonomics is endogenous metabolites (intermediate or metabolic end products), the influence of internal and external factors such as gene expression, protein regulation and the like on the body state can be analyzed from the whole organism by analyzing the change rule of the metabolites in body fluid and tissues, and the change of any physiological, pathological or other factors in the body can influence the concentration of the metabolites or change the metabolic flux, so that the metabonomic technology can reflect the actual situation of the body more truly. The metabolome can be classified into blood, tissue, urine, breath metabolome, etc., depending on the sample source of the metabolite.
The breath metabolome has the characteristics of continuous, noninvasive and convenient sampling in a plurality of metabolome researches. Substances directly discharged from blood through a qi-blood barrier or respiratory tract metabolism are mixed with air to form aerosol, and the aerosol comprises endogenous volatile organic compounds, non-volatile organic compounds, inorganic gas and the like, wherein the endogenous volatile organic compounds are the substances which are most researched by an expiratory metabolome. Comparing the differences of sampling modes determined by sample sources in different metabolome studies, the expiratory metabolome study has the advantage of continuous sampling. Based on the semi-permeability of the qi-blood barrier of an organism, considerable gas-liquid exchange area in the respiratory process, water vapor evaporation and pneumatic atomization of the inner surface liquid film of the lower respiratory tract (bronchioles, respiratory bronchioles, alveolar ducts and the like), the expiratory metabolome not only reflects respiratory tract related tissue metabolism, but also can reflect secondary and systemic metabolism by presenting metabolites in blood.
In a first exemplary embodiment of the present disclosure, an expiratory metabolic assay system is provided. As shown in fig. 1 to 8, before specifically describing the first exemplary embodiment of the present disclosure, it should be mentioned in advance that the first end to the second end are from top to bottom in the vertical direction and from right to left in the horizontal direction.
The disclosed breath metabolism assay system includes: the device comprises an organic vapor micro-injection module 1, an air pump 32, a monomer breath print sampling module 36, an aerosol nozzle 311, a gas electromagnetic valve 35, a zero-level air purifier 42 and an automatic solvent desorption module 2.
The first end of the air pump 32 is connected with the exit port of the organic vapor microinjection module 1, and the exit port of the organic vapor microinjection module 1 outputs organic vapor; the second end of the suction pump 32 is connected to the first end of the liner 33; an air outlet of the single breath print sampling module 36 is connected with the second end of the liner tube 33, an air outlet of the single breath print sampling module 36 is connected with a nozzle interface of the aerosol nozzle 311, and an air inlet of the single breath print sampling module 36 is connected with an air outlet of the zero-order air purifier 42; the liner tube 33 is also provided with a gas shunt electromagnetic valve 34; the gas solenoid valve 35 is connected with the first end of the aerosol nozzle 311, as shown in fig. 13, the gas inlet 351 of the gas solenoid valve 35 is connected with the zero-level air purifier 42, the zero-level air is controlled to enter the monomer breath print sampling module 36 and to synchronously block the inhalation of the exposed substance from entering the monomer breath print sampling module 36, the stirrer adsorption extraction rod is arranged in the liner tube 33 to sample the breath metabolites of the experimental animal, and the automatic solvent desorption module 2 is used for detecting the breath metabolites collected by the stirrer adsorption extraction rod.
The respective components of the breath metabolic assay system are described in detail below.
As regards the organic vapour microinjection module 1, it comprises: the device comprises an injector, a thermostatic bath seat 12, a guide rod, a stress plate 11 and a driving device. The injector is inserted in the groove body of the constant temperature groove seat 12, organic steam is filled in the cavity of the injector, and the constant temperature groove seat 12 has the function of preserving heat or promoting evaporation of the organic steam in the cavity of the injector. The guide rod is fixed on the thermostatic bath seat 12, the stress plate 11 is sleeved on the guide rod, the driving device drives the stress plate 11 to move towards the thermostatic bath seat 12 along the guide rod, and the stress plate 11 presses the injector injection rod, so that the organic steam is output through the injector injection port. In the preparation of the organic vapor, a zero-level air purifier 42 is interfaced with the injector chamber to provide purified air thereto. Wherein, the drive device can be a hydraulic push rod or a mechanical push rod of a stepping motor. The bottom of the zero-order air purifier 42 is provided with a shock absorption leg 41 to reduce the mechanical vibration of the zero-order air purifier 42 during operation.
As shown in fig. 9, the automatic solvent desorption module 2 includes: the device comprises an accommodating cavity, a cover 21, a cover buckle 25, two smooth electromagnetic rods 22, a transmission rubber rod 23, a sample bottle magnetic pipe frame 24, a stepping motor 26 and an desorption process controller 27. The transmission rubber stick 23 sets up between two smooth electromagnetic sticks 22, and the both ends of smooth electromagnetic stick 22 and transmission rubber stick 23 are connected with the wall of holding cavity respectively. The sample bottle is horizontally arranged between the two smooth electromagnetic rods 22, a solvent and the stirrer adsorption extraction rod are filled in the sample bottle, and the stirrer adsorption extraction rod is provided with a magnetic core. The stepping motor 26 electromagnetically drives the smooth electromagnetic rod 22 to rotate. The desorption process controller 27 controls the rotation direction, rotation speed, and rotation time of the sample bottle.
A sample bottle containing a solvent and a stirrer adsorption extraction rod (with a magnetic core) is horizontally placed between the smooth electromagnetic rod 22 and the transmission rubber rod 23, before automatic solvent desorption starts, the direction of the sample bottle is adjusted to enable the direction of the magnetic field of the magnetic core to be the same as the direction of the magnetic field of the smooth electromagnetic rod 22 after electrification, and when the cover 21 and the cover buckle 25 are closed, the stirrer adsorption extraction rod bounces and is separated from the solvent. The transmission rubber rod 23 enables the sample bottle to roll continuously through friction force, the stirring element adsorption extraction rod is soaked and washed by the solvent continuously, the smooth electromagnetic rod 22 is not electrified in the rolling process of the sample bottle, and the sample bottle only plays a supporting role. The stepping motor 26 drives the transmission rubber rod 23 to rotate, and the rotation direction, the rotation speed and the rotation time of the transmission rubber rod 23 are regulated by the desorption process controller 27. When the rotation is finished, the desorption process controller 27 automatically switches on the power supply of the smooth electromagnetic rod 22, the stirring bar adsorption extraction rod is bounced, and the automatic desorption process is finished. Magnets with opposite magnetic field directions are arranged on two sides of each sample bottle magnetic pipe frame 24, the direction of a stirrer adsorbing and extracting rod (with a magnetic core) in each sample bottle does not need to be adjusted, and when the sample bottle ending the desorption process is inserted into the sample bottle magnetic pipe frame 24, the stirrer adsorbing and extracting rod in each sample bottle is attached to the inner wall of the bottle, so that the desorbed solution can be transferred from the bottle opening through a liquid transfer gun conveniently. The gas flow controller 43 is selected to control the gas flow rate in the above-mentioned gas path portion.
In this optional embodiment, the method further includes: and the expiratory print operating cabin 3 is used for accommodating the air suction pump 32, the single expiratory print sampling module 36, the aerosol nozzle 311 and the gas solenoid valve 35.
The expiratory print operating cabin 3 is further described below.
The breath print operating compartment 3 includes: top cover 30, bottom cover 313, two curved sliding doors 31, support bracket 310 and support plate 39. The air pump 32 is arranged on the top cover 30 in a penetrating way and is connected with the emergent port of the organic vapor micro-injection module 1; the second end of the aerosol nozzle 311 is arranged through the bottom cover 313; the top cover 30 and the bottom cover 313 are both internally provided with an inner track and an outer track; the two curved sliding doors 31 are respectively arranged between the top cover 30 and the bottom cover 313, and the two curved sliding doors 31 respectively slide along the inner track and the outer track through rollers; the two ends of the supporting frame 310 are respectively connected with the top cover 30 and the bottom cover 313; the supporting plate 39 is disposed between the top cover 30 and the bottom cover 313, and the supporting plate 39 is inserted into the supporting frame 310. The curved sliding doors 31 are half-moon-shaped and provided with rollers, and the two curved sliding doors 31 with different curvatures can slide along the inner track and the outer track of the top cover 30 and the bottom cover 313 respectively at any angle, thereby facilitating the experiment operation in the breath print operating cabin 3
As shown in fig. 10 and 11, the single expiratory blot sampling module 36 includes: the mask 360, air inlet 3601, gas outlet 3602, experimental animal mouth and nose interface 3603, main pipe 366, moisture absorption strip plate 365.
In an alternative embodiment, the cross-section of the mask 360 is a double-layer lumen structure. The air inlet port 3601 is in communication with a first end of an inner lumen 3604 of the mask 360. The air outlet 3602 is communicated with the outer wall surface of the outer cavity tube of the face mask 360. The experimental animal oral-nasal interface 3603 is a hollow conical tube which is used for enabling the face mask 360 to be in contact with the head of an experimental animal, and it should be noted that the experimental animal oral-nasal interface 3603 is not directly connected with the inner-layer cavity tube 3604 of the face mask 360. When the head of the experimental animal is inserted into the oral-nasal interface 3603, the oral-nasal end of the experimental animal extends out of the oral-nasal interface 3603 and is opposite to the inner cavity tube 3604 of the face mask, and air in the double-layer cavity tube of the face mask 360 can flow from the air inlet 3601 to the air outlet 3602 in a one-way mode.
In an alternative embodiment, the face shield 360 may be coupled to the main tube 366 such that a protrusion on the bottom of the face shield 360 is aligned with a slot in the main tube 366, the slot communicating with the fastening screw hole 362, and the face shield 360 may be fastened to the main tube 366 by screwing the protrusion on the bottom of the face shield 360 through the fastening screw hole 362.
In this optional embodiment, a limiting bolt 361 can be inserted into the main tube 366 near the opening of the face mask 360, the limiting bolt 361 can be used together with the face mask 360 to limit the free movement of the head of the experimental animal, and the size of the opening of the limiting bolt 361 is smaller than the size of the head measured by the experimental animal and larger than the size of the neck, so that the upper respiratory tract of the experimental animal is prevented from being pressed when the movement of the head is limited. Wherein, the tip of the limit bolt 361 has a hemispherical protrusion, which can prevent the limit bolt 361 from sliding off the transparent tube 366.
In the alternative embodiment, a limiting bolt 361 can be inserted into the main tube 366 at an opening far away from the mask 360, so as to ensure that the experimental animal cannot escape from the transparent tube 366 even if the experimental animal is separated from the mask during the operation. In a specific embodiment, the skilled person can customize the inner diameter of the main tube 366 according to the body size of the experimental animal, so as to achieve the effect of preventing the experimental animal from turning around, and the specific limitation is not needed, thereby further reducing the possibility of the experimental animal escaping from the main tube 366.
Wherein, regarding moisture absorption strip 365, the first end of moisture absorption strip 365 with be responsible for 366 first end and piece together to insert and be connected, moisture absorption strip 365 second end is managed 366 and goes up keeping away from the spacing bolt 361 that the trompil department of face guard 360 is inserted and fix, and moisture absorption strip 365 is relative with experimental animals's ventral surface for absorb the urine and keep being responsible for the inside dry of 366.
In this optional embodiment, the method further includes: camera fixed ring 363, camera 364 and respiratory video collector 44.
The fixed ring 363 of camera can be overlapped on being responsible for 366, and the bottom of the fixed ring 363 of camera is connected with camera 364, and camera 364 camera head 364 camera lens point to experimental animals's chest belly for shoot the breathing video, camera 364 and breathing video collector 44 pass through the data line and are connected, receive the experimental animals breathing video that camera 364 shot. The video data stored in the breath video collector 44 can be further used for reflecting the physiological state of the breath of the experimental animal in the expiratory blot after further image processing and machine vision analysis.
Further, in order to better shoot the breath video of the experimental animal, the main tube 366, the moisture absorption strip 365 and the camera fixing ring 363 can be all transparent structures, such as a full transparent structure, a local transparent structure with a transparent main shooting position, and the like.
In an alternative embodiment, the breath metabolism assay system further comprises: and a first end of the multifunctional ejector 312 is connected with a second end of the aerosol nozzle 311, and a second end of the multifunctional ejector 312 is connected with an air outlet of the zero-level air purifier 42.
The multi-functional ejector 312 is further described below, as shown in fig. 14.
The multifunctional ejector 312 includes: an ejector chamber 3125, a liquid level sensor 3124, a gas-liquid interface 3122, a two-phase nozzle 3123, and a turbulence cone 3121. The ejector chamber 3125 is connected to a first end of the gas-liquid interface 3122. The second end of the gas-liquid interface 3122 is connected with the organic vapor micro-injection module 1 and/or an external supplement device, and after the gas-liquid interface 3122 is connected with the organic vapor micro-injection module 1, the gas-liquid interface can be used for the inhalation exposure of organic vapor and the internal standard method quantification of target metabolites. The external supplement device can be selected from a gas cylinder, a liquid supplement device and the like. When the gas-liquid interface 3122 leads solution or suspension into the ejector chamber 3125, the liquid level sensor 3124 is connected with an external liquid replenishing device for controlling the liquid replenishing process. The first end of the two-phase nozzle 3123 extends into the ejector chamber 3125, the second end of the two-phase nozzle 3123 is connected to the air outlet of the zero-order air purifier 42, and the two-phase nozzle 3123 pneumatically atomizes the liquid in the ejector chamber 3125 and generates an aerosol. The turbulence cone 3121 is disposed within the ejector chamber 3125, and the turbulence cone 3121 is disposed opposite the first end of the two-phase nozzle 3123 to impinge on the aerosol within the ejector chamber 3125, thereby eliminating larger particle size particles from the aerosol for inhalation exposure. The two-phase nozzle 3123 cooperates with the turbulence cone 3121 to dilute and mix the organic vapor, gas, or aerosol in the jet chamber 3125.
In an alternative embodiment, the breath metabolism assay system further comprises: and an external standard module 37. The external standard module 37 is used for simulating the expiration process of the experimental animal and the external standard method quantification of the target metabolite. As shown in FIG. 12, an external standard module 37 is coupled to the second end of the liner 33. The organic vapor interface 371 of the external standard module 37 is connected with the air outlet of the zero-order air purifier 42.
In an alternative embodiment, the breath metabolism assay system further comprises: an air background module 38. An air background module 38 is used to detect the air background in the expiratory metabolome system. An air background module 38 is coupled to the second end of liner 33.
So far, the embodiments of the present disclosure have been described in detail with reference to the accompanying drawings. It is to be noted that, in the attached drawings or in the description, the implementation modes not shown or described are all the modes known by the ordinary skilled person in the field of technology, and are not described in detail. Further, the above definitions of the various elements and methods are not limited to the various specific structures, shapes or arrangements of parts mentioned in the examples, which may be easily modified or substituted by those of ordinary skill in the art.
From the above description, those skilled in the art should have a clear understanding of the disclosed expiratory metabolic assay system.
In summary, the present disclosure provides an expiratory metabolic assay system capable of performing a synchronous operation of inhalation exposure and an expiratory blot on an experimental animal, which solves the problem that the inhalation exposure and the expiratory blot are difficult to combine in real time at present, and in the research in the fields of biology, medicine, pharmacy, and the like, the real-time response of the expiratory metabolome change to the inhalation exposure has an irreplaceable effect in revealing the instant health effect of an exogenous substance entering an organism through an inhalation pathway.
It should also be noted that directional terms, such as "upper", "lower", "front", "rear", "left", "right", and the like, used in the embodiments are only directions referring to the drawings, and are not intended to limit the scope of the present disclosure. Throughout the drawings, like elements are represented by like or similar reference numerals. Conventional structures or constructions will be omitted when they may obscure the understanding of the present disclosure.
And the shapes and sizes of the respective components in the drawings do not reflect actual sizes and proportions, but merely illustrate the contents of the embodiments of the present disclosure. Furthermore, in the claims, any reference signs placed between parentheses shall not be construed as limiting the claim.
Furthermore, the word "comprising" does not exclude the presence of elements or steps not listed in a claim. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements.
The use of ordinal numbers such as "first," "second," "third," etc., in the specification and claims to modify a corresponding element does not by itself connote any ordinal number of the element or any ordering of one element from another or the order of manufacture, and the use of the ordinal numbers is only used to distinguish one element having a certain name from another element having a same name.
Similarly, it should be appreciated that in the foregoing description of exemplary embodiments of the disclosure, various features of the disclosure are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the disclosure and aiding in the understanding of one or more of the various disclosed aspects. However, the disclosed method should not be interpreted as reflecting an intention that: that is, the claimed disclosure requires more features than are expressly recited in each claim. Rather, as the following claims reflect, disclosed aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this disclosure.
The above-mentioned embodiments are intended to illustrate the objects, aspects and advantages of the present disclosure in further detail, and it should be understood that the above-mentioned embodiments are only illustrative of the present disclosure and are not intended to limit the present disclosure, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present disclosure should be included in the scope of the present disclosure.
Claims (9)
1. An expiratory metabolism assay system comprising:
the organic vapor micro-injection module outputs organic vapor from an outlet;
the first end of the air pump is connected with the emergent port of the organic vapor micro-injection module; the second end of the air pump is connected with the first end of the liner tube;
the device comprises a single breath print sampling module, an experimental animal is placed in the single breath print sampling module, an air outlet of the single breath print sampling module is connected with the second end of a liner tube, a stirrer adsorption extraction bar is arranged in the liner tube, and breath metabolites of the experimental animal are sampled;
the aerosol nozzle is radially provided with at least one nozzle interface; the nozzle interface is connected with an air outlet of the single breath print sampling module;
the gas electromagnetic valve is connected with the first end of the aerosol nozzle;
the gas outlet of the zero-order air purifier is connected with the gas inlet interface of the gas electromagnetic valve and/or the gas inlet of the monomer breath print sampling module; the gas electromagnetic valve controls zero-level air output by the zero-level air purifier to enter the monomer breath print sampling module and synchronously blocks inhaled exposed substances from entering the monomer breath print sampling module; and
the automatic solvent desorption module is used for detecting the breath metabolites collected by the stirrer adsorption extraction bar;
a first end of the multifunctional ejector is connected with a second end of the aerosol nozzle, and the second end of the multifunctional ejector is connected with an air outlet of the zero-level air purifier; the multifunctional ejector comprises:
the ejector cavity is connected with the first end of the gas-liquid interface; the second end of the gas-liquid interface is respectively connected with the machine steam micro-injection module;
a first end of the two-phase nozzle extends into the ejector chamber, a second end of the two-phase nozzle is connected with an air outlet of the zero-level air purifier, and the two-phase nozzle carries out pneumatic atomization on the liquid in the ejector chamber and generates aerosol; and
the turbulent cone is arranged in the ejector cavity, is opposite to the first end of the two-phase nozzle and is used for impacting the aerosol in the ejector cavity.
2. The exhaled breath metabolic assay system of claim 1, further comprising:
the external standard module is connected with the second end of the liner tube and is used for simulating the expiration process of the experimental animal and external standard method quantification of target metabolites; and an organic steam interface of the external standard module is connected with an air outlet of the zero-order air purifier.
3. The exhaled breath metabolic assay system of claim 1, further comprising:
an air background module coupled to the second end of the liner, the air background module configured to detect an air background in the expiratory metabolic assay system.
4. The expiratory metabolic assay system of claim 1, wherein the monomeric expiratory blot sampling module comprises:
the section of the mask is of a double-layer cavity tube structure;
an air inlet communicated with the first end of the inner cavity tube of the face mask;
the air outlet is communicated with the outer wall surface of the outer cavity tube of the mask;
the first end of the experimental animal oral-nasal interface is connected with the second end of the mask; the head of the experimental animal extends into the second end of the oral-nasal interface of the experimental animal and is opposite to the second end of the inner cavity tube of the face mask;
the first end of the main pipe is connected with the second end of the face mask in a splicing and inserting manner; and
moisture absorption strip shaped plate, moisture absorption strip shaped plate first end with be responsible for first end and piece together to insert the connection, moisture absorption strip shaped plate second end with be responsible for the second end and pass through limit baffle and connect, moisture absorption strip shaped plate with laboratory animal's ventral surface is relative.
5. The exhaled breath metabolic assay system of claim 4, wherein said main tube and said moisture absorbing strip are transparent structures, said unitary exhaled breath blot sampling module further comprising:
the camera fixing ring is sleeved on the outer wall of the main pipe;
the camera is connected with the camera fixing ring; the lens of the camera is opposite to the ventral surface of the experimental animal; and
and the breath video collector is used for receiving the breath video of the experimental animal shot by the camera.
6. The breath metabolism assay system of claim 1, wherein the organic vapor microinjection module comprises:
an injector, wherein the cavity of the injector is filled with organic vapor;
the injector is inserted into the groove body of the constant-temperature groove seat;
the guide rod is fixed on the thermostatic bath seat;
the stress plate is sleeved on the guide rod; and
and the driving device drives the stress plate to move towards the constant-temperature groove seat along the guide rod, and the stress plate presses the injection rod of the injector to enable the organic steam to be output through the injection port of the injector.
7. The exhaled breath metabolic assay system of claim 1, further comprising: the breath print operating cabin is used for accommodating the air pump, the single breath print sampling module, the aerosol nozzle and the gas electromagnetic valve; the breath print operating compartment comprises:
the air suction pump penetrates through the top cover and is connected with the emergent port of the organic vapor micro-injection module;
the second end of the aerosol nozzle penetrates through the bottom cover; the top cover and the bottom cover are both internally embedded with an inner track and an outer track;
the two curved surface sliding doors are arranged between the top cover and the bottom cover and respectively slide along the inner track and the outer track through rollers;
the two ends of the support frame are respectively connected with the top cover and the bottom cover; and
the supporting disk, the supporting disk sets up between top cap and the bottom, just the supporting disk wears to establish on the support frame.
8. The breath metabolic assay system of claim 1, wherein the automatic solvent desorption module comprises:
the accommodating cavity is provided with a plurality of accommodating holes,
the transmission rubber rod is arranged between the two smooth electromagnetic rods, and two ends of the smooth electromagnetic rods and two ends of the transmission rubber rod are respectively arranged in the accommodating cavity in a penetrating manner;
the sample bottle is horizontally arranged between the two smooth electromagnetic rods; a solvent and the stirrer adsorption extraction rod are arranged in the sample bottle, and the stirrer adsorption extraction rod is provided with a magnetic core;
the stepping motor is used for electromagnetically driving the smooth electromagnetic rod to rotate; and
and the desorption process controller controls the rotation direction, the rotation speed and the rotation time of the sample bottle.
9. The breath metabolic assay system of claim 8, wherein the automatic solvent desorption module further comprises:
a sample bottle magnetic pipe frame, wherein the sample bottle after desorption is vertically inserted into the sample bottle magnetic pipe frame; and magnetic fields with opposite magnetic field directions are arranged at two ends of the magnetic pipe frame of each sample bottle, so that the stirrer adsorption extraction rod in each sample bottle is attached to the inner wall of the sample bottle.
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