CN113008880A - Medical instrument residual blood detection kit and using method thereof - Google Patents
Medical instrument residual blood detection kit and using method thereof Download PDFInfo
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- CN113008880A CN113008880A CN202110218965.8A CN202110218965A CN113008880A CN 113008880 A CN113008880 A CN 113008880A CN 202110218965 A CN202110218965 A CN 202110218965A CN 113008880 A CN113008880 A CN 113008880A
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a medical instrument residual blood detection kit and a using method thereof, and relates to the technical field of medical instrument cleaning detection. The invention comprises developer A liquid and developer B liquid, positive contrast liquid, swab and filter paper, external packing box, semi-quantitative standard color card, and a terminal machine for photographing and recording, wherein the terminal machine is used for recording all instrument information and cleaning process information, and the developer A comprises the following components: the color-developing composition A comprises the following raw materials in parts by weight: 2-12 parts of alcohol solvent and 0.2-2 parts of light stabilizer. The blood-collecting device is simple to operate, the swab and the filter paper can be matched to be contacted with any part of any instrument, particularly a cavity, a hollowed part and a gap of the instrument, so that the part to be detected can be directly wiped, and the residual blood quantity of the part to be detected can be accurately fed back.
Description
Technical Field
The invention belongs to the technical field of cleaning and detecting of medical instruments, and particularly relates to a medical instrument residual blood detection kit and a using method thereof.
Background
The cleaning of the reused medical apparatus can reduce the biological load to the maximum extent, the sterilization process does not include any cleaning process, and the dirt of the articles with the dirt continues to exist after the sterilization; any residual organic matter such as blood clots, pus, proteins, mucus, oil stains, etc. can prevent microorganisms from effectively contacting with the sterilization medium and can produce a protective film of bacteria to affect the sterilization effect, resulting in failure of chemical sterilization, gas, steam, hot air, and low temperature sterilization; the accurate and timely cleaning process and the good cleaning result can reduce the chance that the subsequent retreatment personnel, such as the operation of the checking and packaging area, are free from the pollution of pathogenic microorganisms to the maximum extent; the cleaning process is timely and correct, the cleaning result is good, the decontamination can be realized to the maximum extent, the cleaning concentration is reduced, the cleaning quality is improved, and meanwhile, the corrosivity of blood and the like to medical equipment can be reduced to the maximum extent.
Technical Specification for Sterilization (2002 edition) and AMIST79 all indicate that thorough cleaning is the key to ensure successful sterilization or disinfection "
The current commonly used cleaning result detection methods include: 1. visual inspection: the method is simple, convenient and economical, is a main daily monitoring means, and is easy to observe larger particles of stains, water scales, rusts and the like. 2. A magnifying glass method: compared with visual method, the sensitivity is improved by 4 times, and particles and stains with the size of 50 mu m can be seen. 3. Residual protein assay: the method is specific, sensitive and convenient to use; is not interfered by the mechanical treatment mode, such as the action of disinfectants, high temperature and the like; but is expensive and not suitable for routine detection. The culture time of the reagent is strictly observed, and the color is deepened due to too long time, so that the interpretation of the result is interfered. 4. Residual blood detection method: although the method has high sensitivity, the existing product is tested by dropping water at the part to be tested on test paper, so the method is inconvenient to use and is not adopted in a large range.
The patent application with the publication number of CN206177839U discloses a medical instrument residual blood contamination detection and cleaning integrated machine, which comprises a machine body, a conveying mechanism, a detection module and a cleaning module; the body is provided with an accommodating space; the conveying mechanism is arranged at the bottom of the accommodating space; the detection module and the cleaning module are arranged in the accommodating space, the detection module and the cleaning module are positioned above the conveying mechanism, and the detection module comprises a disc, a detection camera, a plurality of spot lamps and a plurality of spray heads; the cleaning module comprises a multi-axis manipulator and a spray gun, wherein the spray gun is arranged at the free end of the multi-axis manipulator, and the spray gun is connected with a dry gas storage tank and a cleaning liquid storage tank. Through being provided with detection module and clean module, utilize detection module can carry out the automated inspection of remaining bloodiness to medical instrument, and utilize clean module can dry medical instrument and can have the medical instrument that remains bloodiness to have and have pointed washing, strengthened the continuity of equipment operation to it is convenient to bring for the use.
The publication is CN205301179U patent application, a medical instrument remains bloodiness detection device is disclosed, the inside top of organism is equipped with sprinkler, sprinkler is connected with the one end of conveyer pipe, the other end of conveyer pipe is connected with detecting the liquid holding vessel, be equipped with the force (forcing) pump on the conveyer pipe, the left and right sides symmetry on the inside top of organism is equipped with first light source and second light source, be equipped with the third light source on the right side inner wall of organism, be equipped with the support slide rail on the left side inner wall of organism, be equipped with the detection trailing arm on the support slide rail, the front end that detects the trailing arm is equipped with and detects camera and data processor, the right side bottom of organism is equipped with data. The beneficial effects of the utility model are that compare with traditional range estimation method, utilize the characteristic that blood and chemical reaction discolour, spray on medical equipment and detect liquid and pass through light source and spectral image processing again, can fix a position fast and remain bloodiness, great promotion the visual sensitivity who detects, the accurate detection of being convenient for remains bloodiness in the medical instrument, has huge latent energy and influence.
The patent application with the publication number of CN2844908Y discloses a device for detecting residual blood stain of medical equipment, which comprises a color development block with the function of detecting trace heme, a substrate and a colorless transparent film, wherein the color development block is attached to the substrate; the colorless transparent film covers part of the surface of the color developing block. The utility model also provides another detection device, including the color development piece that has the function of detecting trace heme and holding the tube-like thing of color development piece, color development piece part sets up in the tube-like thing. The utility model discloses a color development piece with detect trace heme function, to remaining a small amount of bloodiness on medical instrument surface, or the bloodiness of hidden department can effectively detect out fast. The utility model discloses utilized the siphon or the dialysis function that apparent color lump self has guarantees the apparent color lump only has the partial surface direct contact to be detected medical instrument to guarantee that chemical can not pollute medical instrument, reach the purpose of detecting safety.
The patent application with the publication number of CN2826413Y discloses a test paper for detecting the residual blood contamination of medical instruments. The applicant designs a test paper for detecting the residual bloodstain of medical instruments after improving a test paper for detecting whether the excrement or urine of a patient contains trace amount of heme in clinical diagnosis. It not only can be fast to remaining on the medical instrument surface with the a small amount of bloodiness that the naked eye can not see, or the bloodiness of hidden department can effectively detect out, and it can not let harmful chemical reagent direct contact medical instrument moreover, and then reaches the purpose of avoiding polluting medical instrument.
The existing detection technologies for the residual blood of the medical apparatus and instruments CN206177839U and CN205301179U have the following disadvantages: the range of detectable range is limited, both inventions are not suitable for detecting the interior of a device with a cavity, both inventions CN2844908Y and CN2826413Y react with an indicator by the residual blood brought by the water drop, the detection result is not complete residual blood quantity, and is not suitable for detecting irregular surfaces or prominent micro surfaces, and the color developing agent is mentioned to be carcinogenic and is not friendly to operators.
Disclosure of Invention
The invention aims to provide a medical instrument residual blood detection kit and a using method thereof, the operation is simple, the matching of a swab and filter paper can be contacted with any part of any instrument, especially a cavity, a hollowed part and a gap of the instrument, the part to be detected can be directly wiped, the amount of residual blood at the part to be detected can be accurately fed back, and the problems in the prior art are solved.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a medical instrument residual blood detection kit comprises a color developing agent A liquid, a color developing agent B liquid, a positive control liquid, a swab, filter paper, an outer packaging box and a semi-quantitative standard color card;
the terminal machine is used for recording all instrument information and information of the cleaning process;
the color developing liquid A comprises the following components: alcohol solvent, light stabilizer, antioxidant, color developing agent, pH value regulator, chelating agent and deionized water;
the color developing composition A liquid comprises the following raw materials in parts by weight: 2-12 parts of alcohol solvent, 0.2-2 parts of light stabilizer, 0.2-2 parts of antioxidant and 0.5-3 parts of color developing agent, wherein the pH value regulator can be adjusted according to the pH value of the solution A, and the final pH value of the solution A is adjusted to 3.5-6; the content of the chelating agent is 0.4-1.4; adding the deionized water to 100 parts;
the color development liquid B comprises 1-5% of hydrogen peroxide.
Optionally, the alcohol agent is ethanol, propylene glycol, butanol, glycerol, or a combination thereof.
Optionally, the antioxidant is a phenolic antioxidant, and the phenolic antioxidant is Irgnox1010 or TBHQ or BHA or BHT, and the like.
Optionally, the color-developing agent is aminopyrine, and the PH regulator is any one of acetic acid, dilute sulfuric acid and hydrochloric acid or a combination thereof.
Optionally, the swab is: the total length is 1cm-30 cm.
Optionally, the length of the swab head is 0.1cm-10cm, the diameter of the swab head is 1mm-5mm, and the swab is made of nylon, polyester or cellulose through flocking or winding.
Optionally, the filter paper: the thickness is 100-400 microns, and the main component is cellulose.
Optionally, the light stabilizer is a composition of hindered amines/benzotriazoles or phentriazine, and the composition of hindered amines/benzotriazoles or phentriazine is any one of or a composition of tiancheng-zhu 5050, tiancheng-zhu 5115, tiancheng-zhu 5248 and tiancheng-zhu 5341.
Optionally, the chelating agent is ethylenediamine tetraacetic acid, and the positive control solution is a component of bovine blood or sheep blood hemoglobin which is water-soluble, and the concentration of the positive control solution is 0.3-3.0 mg/L.
A method of using a medical device residual blood detection kit, comprising: detecting the interior of a medical instrument with a cavity;
detecting a physical instrument;
wherein, the medical instrument with the cavity has the following internal detection limit and the lowest detection limit of the physical instrument detection: 5 micrograms;
the color does not change after 1 minute after wiping, and the color can be judged to be negative;
a positive control method step;
if the result is positive, it indicates that the reaction activity of A, B solution is effective.
The embodiment of the invention has the following beneficial effects:
the embodiment of the invention has simple operation, and the matching of the swab and the filter paper can be contacted with any part of any instrument, especially the cavity, the hollowed part and the gap of the instrument, so that the part to be detected can be directly wiped, and the residual blood quantity of the part to be detected can be accurately fed back.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic diagram of a terminal according to an embodiment of the invention;
FIG. 2 is a schematic diagram showing a comparative reaction scheme according to an embodiment of the present invention;
FIG. 3 is a schematic view of the color development of the reaction reagent according to one embodiment of the present invention;
FIG. 4 is a diagram of a standard color chart according to an embodiment of the present invention;
FIG. 5 is a schematic view of an embodiment of the present invention;
FIG. 6 is a schematic view of a swab-wiping bioptome in accordance with one embodiment of the present invention;
FIG. 7 is a schematic view of an embodiment of the present invention with filter paper wiped against the outside;
FIG. 8 is a schematic view of the filter paper test outer surface inside the swab wiping chamber according to one embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.
To maintain the following description of the embodiments of the present invention clear and concise, a detailed description of known functions and known components of the invention have been omitted.
Referring to fig. 1-8, in the present embodiment, a medical device blood residual detection kit is provided, including: the color developing agent A solution, the color developing agent B solution and the positive control solution simultaneously comprise a swab and filter paper, an outer packing box and a semi-quantitative standard color card;
the terminal machine is used for recording all instrument information and information of the cleaning process;
the color developing liquid A comprises the following components: alcohol solvent, light stabilizer, antioxidant, color developing agent, pH value regulator, chelating agent and deionized water;
the color developing composition A liquid comprises the following raw materials in parts by weight: the color developing liquid A comprises the following components: alcohol solvent, light stabilizer, antioxidant, color developing agent, pH value regulator, chelating agent and deionized water;
the color developing composition A liquid comprises the following raw materials in parts by weight: 2-12 parts of alcohol solvent, 0.2-2 parts of light stabilizer, 0.2-2 parts of antioxidant and 0.5-3 parts of color developing agent, wherein the pH value regulator can be adjusted according to the pH value of the solution A, and the final pH value of the solution A is adjusted to 3.5-6; the content of the chelating agent is 0.4-1.4; adding the deionized water to 100 parts;
the color development liquid B comprises 1-5% of hydrogen peroxide.
The blood-collecting device is simple to operate, the swab and the filter paper can be matched to be contacted with any part of any instrument, particularly a cavity, a hollowed part and a gap of the instrument, so that the part to be detected can be directly wiped, and the residual blood quantity of the part to be detected can be accurately fed back.
The alcohol agent in this embodiment is ethanol, propylene glycol, butanol, glycerol, or a combination thereof.
The antioxidant in this embodiment is a phenolic antioxidant, and the phenolic antioxidant is Irgnox1010 or TBHQ or BHA or BHT, etc.
The color-developing agent in this embodiment is aminopyrine, and the PH adjuster is any one of acetic acid, dilute sulfuric acid, and hydrochloric acid, or a combination thereof.
The swab of the present embodiment: the total length is 1cm-30 cm.
The length of the swab head is 0.1cm-10cm, the diameter is 1mm-5mm, and the swab is made of nylon, polyester or cellulose and is made in a flocking type or a winding type.
The filter paper of this example: the thickness is 100-400 microns, and the main component is cellulose.
The light stabilizer of this embodiment is a composition of hindered amines/benzotriazoles or benzotriazines, and the composition of hindered amines/benzotriazoles or benzotriazines is any one of tiancheng zhu 5050, tiancheng zhu 5115, tiancheng zhu 5248 and tiancheng zhu 5341 or a composition thereof.
The chelating agent of the embodiment is ethylenediamine tetraacetic acid, and the component of the positive control solution, namely bovine blood or sheep blood hemoglobin, is water-soluble, and the concentration of the chelating agent is 0.3-3.0 mg/L.
A method of using a medical device residual blood detection kit, comprising: detecting the interior of a medical instrument with a cavity;
detecting a physical instrument;
wherein, the medical instrument with the cavity has the following internal detection limit and the lowest detection limit of the physical instrument detection: 5 micrograms;
the color does not change after 1 minute after wiping, and the color can be judged to be negative;
a positive control method step;
if the result is positive, the reaction activity of A, B solution is effective;
as shown in fig. 1, the color development result after the swab and the filter paper are wiped can be photographed by a terminal machine, and then the test result is printed and recorded;
the blood-collecting device is simple to operate, the swab and the filter paper can be matched to be contacted with any part of any instrument, particularly a cavity, a hollowed part and a gap of the instrument, so that the part to be detected can be directly wiped, and the residual blood quantity of the part to be detected can be accurately fed back. For example, the gynecological aspirator can be used for detecting residual blood, the inside of the gynecological aspirator can be wiped by using A/B liquid in combination with a swab, and the outside of the gynecological aspirator can be wiped by using A/B liquid in combination with filter paper, so that the gynecological aspirator can be operated conveniently. For example, the residual blood at the meshing part of the hemostatic forceps can be tested by clamping filter paper wetted by A/B liquid, so that whether the residual blood exists can be judged, and the hemostatic forceps are visual and easy to operate. The head of the biopsy forceps is provided with a gap which is a cleaning blind area, so that a hand is difficult to directly contact, the biopsy forceps can be extended into the cavity by using a swab, and the biopsy forceps are repeatedly wiped to detect residual blood.
As shown in FIG. 5, among them, 1 swab, 1-1 swab head, 1-2 swab rod, 2 filter paper, 3 color reagent A solution, 4 color reagent B solution, 5 positive control, 6 hand-held terminal mechanism, 7 external packing box.
The above embodiments may be combined with each other.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are capable of operation in sequences other than those illustrated or described herein.
In the description of the present invention, it is to be understood that the orientation or positional relationship indicated by the orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a reverse description, these orientation words do not indicate and imply that the device or element being referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be considered as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.
Claims (10)
1. A medical device residual blood detection kit, comprising: the color developing agent A solution, the color developing agent B solution and the positive control solution simultaneously comprise a swab and filter paper, an outer packing box and a semi-quantitative standard color card;
the terminal machine is used for recording all instrument information and information of the cleaning process;
the color developing liquid A comprises the following components: alcohol solvent, light stabilizer, antioxidant, color developing agent, pH value regulator, chelating agent and deionized water;
the color developing composition A liquid comprises the following raw materials in parts by weight: 2-12 parts of alcohol solvent, 0.2-2 parts of light stabilizer, 0.2-2 parts of antioxidant and 0.5-3 parts of color developing agent, wherein the pH value regulator can be adjusted according to the pH value of the solution A, and the final pH value of the solution A is adjusted to 3.5-6; the content of the chelating agent is 0.4-1.4; adding the deionized water to 100 parts;
the color development liquid B comprises 1-5% of hydrogen peroxide.
2. The medical device residual blood detection kit according to claim 1, wherein the alcohol agent is ethanol, propylene glycol, butanol, glycerol, or a combination thereof.
3. The medical device residual blood detection kit according to claim 2, wherein the antioxidant is a phenolic antioxidant, and the phenolic antioxidant is Irgnox1010 or TBHQ or BHA or BHT.
4. The kit according to claim 3, wherein the color-developing agent is aminopyrine, and the pH regulator is any one of acetic acid, dilute sulfuric acid and hydrochloric acid or a combination thereof.
5. The medical device residual blood detection kit according to claim 4, wherein the swab: the total length is 1cm-30 cm.
6. The medical device residual blood detection kit according to claim 5, wherein the swab head has a length of 0.1cm-10cm and a diameter of 1mm-5mm, and the swab is made of nylon, polyester or cellulose and is made by flocking or winding.
7. The medical device residual blood detection kit according to claim 6, wherein the filter paper: the thickness is 100-400 microns, and the main component is cellulose.
8. The kit for detecting residual blood in medical equipment according to claim 7, wherein the light stabilizer is a hindered amine/benzotriazole or benzotriazine composition, and the hindered amine/benzotriazole or benzotriazine composition is one of tiancheng kang 5050, tiancheng kang 5115, tiancheng kang 5248 and tiancheng kang 5341 or a combination thereof.
9. The medical device residual blood detection kit according to claim 8, wherein the chelating agent is ethylenediamine tetraacetic acid, and the positive control solution is a component of bovine or sheep blood hemoglobin soluble in water at a concentration of 0.3-3.0 mg/L.
10. A method for using a medical device residual blood detection kit, comprising: detecting the interior of a medical instrument with a cavity;
step 1, taking a swab, firstly dripping 2-3 drops of liquid A on a swab head, and then dripping 2-3 drops of liquid B on the same position to wet the swab head;
step 2, the moistened wiper head is immediately used for penetrating into the cavity mirror to be detected, rotating wiping is carried out, and the wiping process is fully contacted with the position to be detected as much as possible;
step 3, observing the color change of the swab, and interpreting after 1 minute after wiping;
step 4, photographing and recording by using a terminal, uploading to a computer, and printing a recording result;
detecting a physical instrument;
step 1, taking a piece of filter paper, dripping 1 drop of liquid A on the filter paper, and dripping 1 drop of liquid B on the same position;
step 2, wiping the part to be detected with the wet part of the filter paper, and wiping repeatedly to ensure that the residues are fully contacted with the color development liquid;
step 3, observing the color change on the filter paper, and comparing the color change with a standard color chart;
step 4, judging and reading after 1 minute after wiping;
step 5, photographing and recording by using a terminal, uploading to a computer, and printing a recording result;
wherein, the medical instrument with the cavity has the following internal detection limit and the lowest detection limit of the physical instrument detection: 5 micrograms;
the color does not change after 1 minute after wiping, and the color can be judged to be negative;
a positive control method step;
step 1, taking a swab, dripping two drops of liquid A on a swab head, dripping two drops of liquid B on the same position, and wetting the swab head
Step 2, dropping a drop of positive control liquid to the swab head;
step 3, observing the color change of the swab and comparing the color change with a standard color card;
if the result is positive, it indicates that the reaction activity of A, B solution is effective.
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| CN202110218965.8A CN113008880A (en) | 2021-02-26 | 2021-02-26 | Medical instrument residual blood detection kit and using method thereof |
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