CN113008754A - Device and method for detecting bacteria filtering efficiency of mask fabric - Google Patents

Device and method for detecting bacteria filtering efficiency of mask fabric Download PDF

Info

Publication number
CN113008754A
CN113008754A CN202110174586.3A CN202110174586A CN113008754A CN 113008754 A CN113008754 A CN 113008754A CN 202110174586 A CN202110174586 A CN 202110174586A CN 113008754 A CN113008754 A CN 113008754A
Authority
CN
China
Prior art keywords
box
drawer
test tube
liquid
type dish
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202110174586.3A
Other languages
Chinese (zh)
Other versions
CN113008754B (en
Inventor
钟宁远
李君�
沈佳琪
朱希
朱辉
公伟
王建东
韦博君
朱央央
谢浙裕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shaoxing Institute For Food And Drug Control
Original Assignee
Shaoxing Institute For Food And Drug Control
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shaoxing Institute For Food And Drug Control filed Critical Shaoxing Institute For Food And Drug Control
Priority to CN202110174586.3A priority Critical patent/CN113008754B/en
Publication of CN113008754A publication Critical patent/CN113008754A/en
Application granted granted Critical
Publication of CN113008754B publication Critical patent/CN113008754B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/08Investigating permeability, pore-volume, or surface area of porous materials
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B25HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25JMANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J21/00Chambers provided with manipulation devices
    • B25J21/02Glove-boxes, i.e. chambers in which manipulations are performed by the human hands in gloves built into the chamber walls; Gloves therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/08Investigating permeability, pore-volume, or surface area of porous materials
    • G01N2015/084Testing filters
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Robotics (AREA)
  • Mechanical Engineering (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a bacteria filtration efficiency detection device of a mask fabric, which comprises a detection cabinet and an aerosol pipe, wherein a negative pressure test cavity is arranged in the detection cabinet, a sealing movable door is arranged on the detection cabinet and can seal the negative pressure test cavity, the aerosol pipe is fixed in the middle of the negative pressure test cavity, a mist liquid generating device is connected with a pipeline at the upper end of the aerosol pipe, and two sampling detection devices are connected with a pipeline at the lower end of the aerosol pipe And (4) cleaning efficiency.

Description

Device and method for detecting bacteria filtering efficiency of mask fabric
Technical Field
The invention relates to the technical field of quality detection devices of mask fabrics, in particular to a device and a method for detecting the bacterial filtration efficiency of a mask fabric.
Background
The mask is a common air filtering appliance, the main performance index is the filtering efficiency of bacteria, the staphylococcus aureus method is generally adopted to test the index, the detection process is to install the mask fabric on a testing device, gas containing bacteria is introduced, after the mask fabric is filtered, part of the bacteria is remained in the testing device, the filtering efficiency of the mask fabric is determined by the culture technology and bacterial colony data acquisition, the staphylococcus aureus is used in the detection process, detection personnel need to wear protective equipment to operate, the experiment process is time-consuming and labor-consuming and has certain danger, the common sampling testing device can be divided into a multilayer culture structure, but the disassembly and the assembly of the testing device are carried out integrally, and a certain culture layer cannot be extracted independently for culture and data acquisition, the disassembly, assembly and cleaning are very inconvenient.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a device and a method for detecting the bacteria filtering efficiency of mask fabric.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a bacterium filtration efficiency detection device of gauze mask surface fabric, is including detecting cabinet, aerial fog pipe, be equipped with negative pressure test chamber in the detection cabinet, detect and install sealed dodge gate on the cabinet, sealed dodge gate can airtight negative pressure test chamber, aerial fog pipe fixes at negative pressure test chamber middle part, aerial fog pipe upper end tube coupling has fog liquid generating device, aerial fog pipe lower extreme tube coupling has two sets of sampling detection device, sampling detection device includes sampling device base, steamer tray box installation screw rod, two steamer tray box installation screw rod parallel arrangement and install perpendicularly on the sampling device base, two the stack is equipped with a plurality of steamer tray box subassembly on the steamer tray box installation screw rod, steamer tray box installation screw rod upper end screw thread is installed and is compressed tightly the spiral shell, through compress tightly the spiral shell can compress tightly a plurality of steamer tray box subassembly coincide.
Two operating gloves are installed on the sealing movable door, and an observation window is arranged on the sealing movable door.
The fog liquid generating device comprises a vibration liquid mixing mechanism, a peristaltic pump and a sprayer, wherein the vibration liquid mixing mechanism comprises a test tube box, a test liquid test tube, a test tube box underframe and a test tube box vibration driving assembly, the test tube box outer side wall is symmetrically connected with test tube box moving wings, the test liquid test tube is inserted into the test tube box, the test tube box underframe is fixedly installed on the inner wall of a negative pressure test cavity, two test tube box moving grooves which are symmetrically arranged are formed in the test tube box underframe, the size of each test tube box moving groove is matched with that of each test tube box moving wing, the test tube box is movably installed on the test tube box underframe through the test tube box moving wings, two impact rods are connected to the bottom of the test tube box, an impact rod moving groove is formed in the test tube box underframe, the test tube box is installed and inserted into the impact rod moving groove, and the test tube box vibration driving assembly comprises a motor, a liquid outlet, the cam, motor fixed mounting is on negative pressure test intracavity wall, the cam is fixed on the motor output shaft, the cam is located between two striker rods, two the striker rod is in on the cam movement track line, peristaltic pump input end links to each other with fungus liquid test tube way, be equipped with fungus liquid import, spraying export, raffinate recovery mouth on the atomizer, peristaltic pump output end links to each other with fungus liquid import pipeline, raffinate recovery mouth links to each other with fungus liquid test tube way, aerial fog pipe top is equipped with the fog import, the fog import links to each other with spraying export pipeline.
The aerial fog pipe top is equipped with auxiliary air inlet, two auxiliary air inlet divides and lists fog liquid import both sides, aerial fog pipe middle part is equipped with the pressure detection interface, aerial fog socle portion is equipped with two symmetrical arrangement's fog liquid export, every the fog liquid export all the tube coupling to on one set of sampling detection device.
The drawer dish box assembly comprises a drawer dish box base, a drawer dish box and a box pressing cover, wherein two drawer dish box moving groove rails are symmetrically arranged on the drawer dish box base, a box body rear limiting seat is connected behind the drawer dish box moving groove rails, drawer dish box moving wings are symmetrically connected to the outer wall of the drawer dish box, the size and the installation position of each drawer dish box moving wing are matched with those of the drawer dish box moving groove rails, the drawer dish boxes are movably inserted into the drawer dish box base through the drawer dish box moving wings, a cover closing groove is formed in the box body rear limiting seat, two drawer dish box moving groove rails are connected to the side faces of the drawer dish box moving groove rails respectively, spring holes which are vertically communicated are formed in the bottom butt joint columns, springs are installed in the spring holes, the box pressing cover comprises a pressing cover plate, a circle of cloth pressing flange is connected to the lower end face of the pressing cover plate, and the peripheral size of the circle of cloth pressing flange is matched with that of the opening of the drawer dish box, the pressure apron front end is connected with the steamer tray board, the pressure apron rear end is connected with the closing and covers the picture peg, close and cover picture peg size and close the cover groove phase-match, the pressure apron both sides are connected with the engaging lug that the symmetry set up, the engaging lug lower extreme is connected with the guide post, be equipped with the double-screw bolt jack in the guide post, the double-screw bolt jack runs through guide post and engaging lug, double-screw bolt jack diameter and steamer tray dish box installation screw rod phase-match, guide post external diameter and spring hole phase-match, install the handle on the outer wall before the steamer tray dish box, be equipped with first joint strip on the steamer tray dish box lateral wall up end.
The bottom of the drawer dish box of top layer and middle layer is equipped with leads to the fog district, it is equipped with a plurality of little through-holes to lead to the fog district, above-mentioned all be equipped with second joint strip on the bottom face of drawer dish box, second joint strip circle is established and is being led to the fog district periphery, middle layer and bottom the box is pressed and is covered and has been seted up and lead to the fog drill way, lead to the fog drill way area and be greater than and lead to the fog district area, lead to the fog drill way area and be less than the inside area of second joint strip, the top layer the box is pressed and is covered and be connected with the sampling and advance the fog interface, the top layer install the surface fabric frame mount table in the drawer dish box, fixed mounting has the surface fabric pavement frame on the surface fabric frame mount table, the surface fabric pavement frame is last to be laid and is equipped with the gauze mask surface.
A method for detecting the bacterial filtration efficiency of mask fabric comprises the following steps:
1) sequentially stacking a plurality of drawer-type dish box assemblies on a sampling device base to form a sampling detection device, loading cut mask fabric to be detected into the drawer-type dish box assembly at the top layer, and rotating a pressing screw disc to press the drawer-type dish box assemblies;
2) inserting a plurality of bacteria liquid test tubes on a test tube box, and well performing the connection work of each pipeline;
3) the test tube box oscillation driving assembly is started to drive the bacterial liquid test tube to oscillate left and right to perform liquid mixing operation, then the peristaltic pump and the sprayer are started, the bacterial liquid is formed into vaporific aerosol and is introduced into the aerosol tube, and the vaporific aerosol is conveyed to the sampling detection device through the aerosol tube;
4) after experiment a period of time, each equipment in step 3) is closed, after a period of standing, upwards twist and compress tightly the spiral shell dish, remove the state that compresses tightly of steamer tray dish subassembly, extract one or more steamer tray dish boxes and go to carry out the bacterial culture, observe, gather bacterial colony data.
The invention has the beneficial effects that: the sampling detection device adopts a drawer type structure, has good sealing performance, can ensure the smooth running of the experiment, can realize the independent disassembly of the drawer type containers in each layer in the data acquisition process, provides convenience for the collection of experiment data, and improves the disassembly, assembly and cleaning efficiency of the device.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a front view of the oscillating liquid mixing mechanism of the present invention;
FIG. 3 is a side view of the oscillating liquid mixing mechanism of the present invention;
FIG. 4 is a front view of the sampling test device of the present invention;
FIG. 5 is a cross-sectional structural view of the top drawer capsule assembly of the present invention;
FIG. 6 is a cross-sectional structural view of the mid-tier drawer capsule assembly of the present invention;
FIG. 7 is a three-dimensional block diagram of the cartridge gland on the top drawer capsule assembly of the present invention;
FIG. 8 is a three-dimensional block diagram of the cartridge gland on the mid-tier drawer capsule assembly of the present invention;
FIG. 9 is a three-dimensional block diagram of the capsule base of the present invention;
fig. 10 is a three-dimensional structural view of the drawer box of the present invention.
In the figure: the detection cabinet 1, the negative pressure test chamber 11, the sealing movable door 12, the operation glove 121, the observation window 122, the aerosol tube 2, the aerosol inlet 21, the auxiliary airflow inlet 22, the pressure detection interface 23, the aerosol outlet 24, the aerosol generating device 3, the oscillation liquid mixing mechanism 31, the test tube box 311, the test tube box moving wing 3111, the striking rod 3112, the bacteria liquid test tube 312, the test tube box bottom frame 313, the test tube box moving groove 3131, the striking rod moving groove 3132, the test tube box oscillation driving component 314, the motor 3141, the cam 3142, the peristaltic pump 32, the atomizer 33, the bacteria liquid inlet 331, the spray outlet 332, the residual liquid recycling port 333, the sampling detection device 4, the sampling device base 41, the drawer box mounting screw 42, the drawer box assembly 43, the drawer box base 431, the drawer box moving groove rail 4311, the box rear limit seat 4312, the close cover groove 4313, the bottom butt joint column 4314, the spring hole 43141, the spring 4315, the drawer box 432, the drawer box moving wing 4321, the drawer box moving wing 4321, the drawer moving wing, The device comprises a handle 4322, a first sealing rubber strip 4323, a second sealing rubber strip 4324, a fog passing area 4325, a small through hole 43251, a box pressing cover 433, a pressing cover plate 4331, a cloth pressing flange 4332, a drawer baffle 4333, a cover closing inserting plate 4334, a connecting lug 4335, a guide column 4336, a stud inserting hole 43361, a fog passing hole 4337, a sampling fog inlet port 4338, a fabric frame mounting table 434, a fabric paving frame 435, an air outlet 436 and a pressing screw disk 44.
Detailed Description
The present invention will be further described with reference to the following drawings and detailed description, wherein the descriptions of "left", "right", etc. refer to fig. 1 for reference:
as shown in figures 1-10, a device for detecting the bacteria filtration efficiency of a mask fabric, which comprises a detection cabinet 1 and an aerosol pipe 2, wherein a negative pressure test cavity 11 is arranged in the detection cabinet 1, the negative pressure test cavity 11 can effectively ensure that no bacteria-carrying gas leaks in an experiment and protect the health of experimenters, a sealing movable door 12 is arranged on the detection cabinet 1, the sealing movable door 12 can seal the negative pressure test cavity 11, all operations must be performed under the condition of closing the sealing movable door 12 during the experiment, the aerosol pipe 2 is fixed in the middle of the negative pressure test cavity 11, the aerosol pipe 2 is used as an intermediate channel of bacteria-carrying aerosol, the upper end of the aerosol pipe 2 is connected with an aerosol generating device 3, the lower end of the aerosol pipe 2 is connected with two sets of sampling detection devices 4, the bacteria-carrying aerosol is generated from the aerosol generating device 3 and flows to the sampling detection device 4 through the aerosol pipe 2, and the sampling detection devices 4 comprise sampling, Drawer dish box installation screw rod 42, two drawer dish box installation screw rods 42 parallel arrangement and vertical installation are on sampling device base 41, two drawer dish box installation screw rods 42 are gone up the fold and are equipped with a plurality of drawer dish box subassembly 43, drawer dish box installation screw rod 42 upper end screw thread is installed and is compressed tightly spiral shell 44, can compress tightly a plurality of drawer dish box subassemblies 43 coincide through compressing tightly spiral shell 44, treat the gauze mask surface fabric fixed mounting who treats the side of trying to see in the drawer dish box subassembly 43 at the top layer, the area fungus aerial fog is filtered through the gauze mask surface fabric after, its residual that is not filtered can be followed the air current and is attacked whereabouts by drawer dish box subassembly 43 one by one, the thallus can be followed the striking and stay on drawer dish box subassembly 43, through carrying out the bacterial culture to drawer dish box subassembly 43, gather the bacterial colony quantity of each drawer dish box subassembly 43 and calculate the bacterial filtration efficiency of gauze mask surface fabric.
Install two operation gloves 121 on the sealed dodge gate 12, because the operation of opening the door is not allowed in the experimentation, all operations need all to go on after operation gloves 121 on the people's gloves, are equipped with observation window 122 on the sealed dodge gate 12, can be in the experimental procedure audio-visual observation experimental conditions.
The fog liquid generating device 3 comprises a vibration liquid mixing mechanism 31, a peristaltic pump 32 and an atomizer 33, the vibration liquid mixing mechanism 31 comprises a test tube box 311, a bacteria liquid test tube 312, a test tube box bottom frame 313 and a test tube box vibration driving component 314, test tube box moving wings 3111 are symmetrically connected on the outer side wall of the test tube box 311, a plurality of bacteria liquid test tubes 312 are inserted in the test tube box 311, bacteria liquid is filled in the bacteria liquid test tubes 312, the test tube box bottom frame 313 is fixedly arranged on the inner wall of the negative pressure test cavity 11, two symmetrically arranged test tube box moving grooves 3131 are arranged on the test tube box bottom frame 313, the size of the test tube box moving groove 3131 is matched with that of the test tube box moving wings 3111, the test tube box 311 is movably arranged on the test tube box bottom frame 313 through the test tube box moving wings 3111, two impact bars 3112 are connected at the bottom of the test tube box 311, impact bar moving groove 3132 is arranged on the test tube box bottom frame 313, after the test tube box 311 is arranged, the impact, the test tube box vibration driving component 314 comprises a motor 3141 and a cam 3142, the motor 3141 is fixedly arranged on the inner wall of the negative pressure test cavity 11, the cam 3142 is fixed on the output shaft of the motor 3141, the cam 3142 is arranged between two impact rods 3112, the two impact rods 3112 are positioned on the motion track line of the cam 3142, because the experiment time is often long, the bacteria liquid is required to be continuously introduced in the process to form aerial fog, the bacteria liquid is easy to form uneven conditions in the test tube under long-time static conditions to influence the output, the vibration liquid mixing mechanism 31 is designed, the motor 3141 drives the cam 3142 to rotate, so that the two impact rods 3112 impact left and right circularly to drive the test tube box 311 to move left and right on the test tube box bottom 313, the bacteria liquid in the test tube 312 is continuously vibrated along with the test tube box 311 moving left and right circularly, the mixing state is maintained, the input end of the peristaltic pump 32, the sprayer 33 is provided with a bacteria liquid inlet 331, a spray outlet 332 and a residual liquid recycling port 333, the output end of the peristaltic pump 32 is connected with a bacteria liquid inlet 331 through a pipeline, the peristaltic pump 32 sucks and conveys the bacteria liquid to the sprayer 33, the residual liquid recycling port 333 is connected with a bacteria liquid test tube 312 through a pipeline, the top of the aerosol tube 2 is provided with a mist liquid inlet 21, the mist liquid inlet 21 is connected with a spray outlet 332 through a pipeline, the sprayer 33 forms the bacteria liquid into mist aerosol to be output into the aerosol tube 2, the sprayer 33 has residual bacteria liquid during aerosol formation, and the residual bacteria liquid flows back into the bacteria liquid test tube 312 through the residual liquid recycling port 333.
2 tops of aerial fog pipe are equipped with auxiliary air inlet 22, two auxiliary air inlet 22 divide and are listed as fog liquid import 21 both sides, auxiliary air inlet 22 links outside clean air supply outward, come and take the fungus aerial fog to mix through letting in clean air, form the air flue of flow direction sampling detection device 4, 2 middle parts of aerial fog pipe are equipped with pressure measurement interface 23, in the experimentation, must keep the negative pressure state in the aerial fog pipe 2, form from last air current passageway extremely down, pressure measurement interface 23 links pressure sensor outward, can survey aerial fog pipe 2's negative pressure, thereby provide the parameter for adjustment air current speed, 2 bottoms of aerial fog pipe are equipped with two symmetrical arrangement's fog liquid export 24, every fog liquid export 24 all pipeline connection is to on one set of sampling detection device 4.
The drawer dish box assembly 43 comprises a drawer dish box base 431, a drawer dish box 432 and a box pressing cover 433, two drawer dish box moving groove rails 4311 which are symmetrically arranged are arranged on the drawer dish box base 431, a box body rear limiting seat 4312 is connected behind the drawer dish box moving groove rails 4311, drawer dish box moving wings 4321 are symmetrically connected to the outer wall of the drawer dish box 432, the dimensions and the installation positions of the drawer dish box moving wings 4321 are matched with those of the drawer dish box moving groove rails 4311, the drawer dish box 432 is movably inserted into the drawer dish box base 431 through the drawer dish box moving wings 4321, the drawer dish box 432 is installed on the drawer dish box base 431 by adopting a drawing and moving structure, the assembly and disassembly are very convenient, the box body rear limiting seat 4312 plays a limiting and positioning role of being installed in place, a closing groove 4313 is arranged on the box body rear limiting seat 4312, both side surfaces of the two drawer dish box moving grooves 4311 are connected with a bottom butt-joint column 4314, a spring hole 43141 is arranged in a butt joint column 4314, the box pressing cover 433 comprises a pressing cover plate 4331, a circle of cloth pressing flange 4332 is connected to the lower end face of the pressing cover plate 4331, the outer periphery of the circle of cloth pressing flange 4332 is matched with the size of the mouth of the drawer dish box 432, after the box pressing cover 433 is installed on the drawer dish box 432, the cloth pressing flange 4332 is inserted into the mouth of the drawer dish box 432, the front end of the pressing cover 4331 is connected with a drawer blocking plate 4333, the rear end of the pressing cover 4331 is connected with a cover closing insertion plate 4334, the size of the cover closing insertion plate 4334 is matched with a cover closing groove 4313, two sides of the pressing cover 4331 are connected with symmetrically arranged connection lugs 4335, the lower end of the connection lug 4335 is connected with a guide column 4336, a stud insertion hole 43361 is arranged in the guide column 4336, the stud insertion hole 43361 penetrates through the guide column 4336 and the connection lug 4335, the diameter of the stud insertion hole 43361 is matched with the mounting screw 42 of the drawer dish box, the outer diameter of the guide column 4336 is matched with the spring hole 43141, and the guide column 4336 plays, a drawer box mounting screw 42 penetrates through a guide column 4336 and a bottom butt joint column 4314, so that the whole drawer box assembly 43 is mounted on the drawer box mounting screw 42, a pressing screw disk 44 is rotated to press a box pressing cover 433 downwards, the guide column 4336 can compress a spring 4315, after the cover is mounted, a drawer blocking plate 4333 can block the pulling-out movement of a drawer box 432, a cover closing inserting plate 4334 is inserted into a cover closing groove 4313, a drawer box base 431, the drawer box 432 and a box pressing cover 433 form a sealed whole, a handle 4322 is mounted on the front outer wall of the drawer box 432 to play a role of the drawer box 432, a first sealing rubber strip 4323 is mounted on the upper end face of the side wall of the drawer box 432, after the box pressing cover 433 is pressed in place, the first sealing rubber strip 4323 plays a role in sealing the joint of the drawer box 432 and the box pressing cover, the leakage of bacteria-carrying aerosol is prevented, and when the drawer box and the bacteria culture box are required to be taken out and the pressing screw disk is reversely pressed, only required to collect the data of the drawer box 432, box gland 433 can lose the overdraft, receive spring 4315 restoring force effect, box gland 433 can be bounced, drawer plate 4333 can no longer block the motion of pulling out of steamer tray box 432, at this moment, just can pull out steamer tray box 432 through handle 4322, the whole dismantlement and the installation of steamer tray box 432 are all very convenient, and in the sampling process, do not need whole sample detection device 4 of tearing open greatly, only need loosen and compress tightly spiral shell 44, just can realize the independent extraction of steamer tray box 432 on each steamer tray box subassembly 43, the sampling operation has been made things convenient for, can realize that corresponding data of gathering individual layer or multilayer carry out the operation of analysis.
The bottom of the drawer box 432 of the top layer and the middle layer is provided with a mist passing area 4325, the mist passing area 4325 is provided with a plurality of small through holes 43251, bacteria-carrying aerosol passes through the plurality of small through holes 43251 of the mist passing area 4325, thalli advancing along with air flow can be left in the mist passing area 4325 during impact so as to facilitate subsequent bacteria culture, the bottom end face of the drawer box 432 is provided with a second sealing rubber strip 4324, the second sealing rubber strip 4324 is arranged around the periphery of the mist passing area 4325 in a circle mode, mist passing holes 4337 are formed in box pressure covers 433 of the middle layer and the bottom layer so as to facilitate the passing of the aerosol, the area of each mist passing hole 4337 is larger than that of the mist passing area 4325, the area of each mist passing hole 4337 is smaller than that of the second sealing rubber strip 4324, during experiments, a gap sealing effect between the drawer box assemblies 43 which are overlapped is realized by the second sealing rubber strips 4324, the gaps between the aerosol passing holes which are mainly prevented from being not leaked from the mist passing holes, and sampling inlet ports, the fog inlet port 4338 is the same as the pipeline of the fog liquid outlet 24 at the bottom of the fog pipe 2, the bacteria-carrying fog enters from the fog inlet port 4338, the surface fabric frame mounting table 434 is mounted in the drawer box 432 at the top layer, the surface fabric spreading frame 435 is fixedly mounted on the surface fabric frame mounting table 434, the surface fabric frame mounting table 434 is a ring and is connected to the inner side wall of the drawer box 432, the surface fabric spreading frame 435 is spread with the mask fabric to be detected, the surface fabric spreading frame 435 adopts a large grid structure, the spreading of the surface fabric is not influenced, the downward permeation after the fog filtration is not influenced, when the box pressing cover 433 is pressed in place, the cloth pressing flange 4332 is pressed on the mask fabric, the position of the mask fabric is pressed and positioned, a sealing glue layer can be mounted at the bottom of the cloth pressing flange 4332 to assist in sealing, the side wall of the drawer box 432 at the bottom layer is connected with the air outlet 436, the drawer box 432 at the bottom layer is not provided with the fog passing region 43, thus, a negative pressure air path from the aerosol pipe 2 to the sampling detection device 4 can be formed, and the flow of the aerosol with bacteria is formed to carry out the whole experiment.
A method for detecting the bacterial filtration efficiency of mask fabric comprises the following steps:
1) sequentially stacking a plurality of drawer box assemblies 43 on a sampling device base 41 to form a sampling detection device 4, loading cut mask fabric to be detected into the drawer box assembly 43 at the top layer, and pressing the plurality of drawer box assemblies 43 by rotating a pressing screw disk 44;
2) inserting a plurality of bacteria liquid test tubes 312 on the test tube box 311, and well performing the connection work of each pipeline;
3) the test tube box oscillation driving assembly 314 is started to drive the bacteria liquid test tube 312 to oscillate left and right to perform liquid mixing operation, then the peristaltic pump 32 and the sprayer 33 are started, the bacteria liquid is formed into fog aerosol and is led into the aerosol tube 2, the fog aerosol formed by the bacteria liquid is mixed with clean air in the aerosol tube 2 to form aerosol, negative pressure is formed in the aerosol tube 2, and the bacteria-carrying aerosol is conveyed to the sampling detection device 4 through the aerosol tube 2;
4) after experiment for a period of time, each device in step 3) is closed, after standing for a period of time, upwards screwing and pressing the screw disc 44, the pressing state of the drawer dish box assembly 43 is removed, one or more drawer dish boxes 432 are extracted to carry out bacteria culture, the bacterial colony data are observed and collected, during the experiment, mask fabrics with different materials and different specifications of layers can be respectively filled in the two sets of sampling detection devices 4 to be tested, the experiment efficiency can be effectively improved, the mask fabrics are not filled in the one set of sampling detection devices 4, the experiment data of the mask fabrics are compared by collecting the bacterial colony number in the empty sampling detection devices 4, and therefore the bacteria filtering efficiency of the mask fabrics is obtained.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (7)

1. The utility model provides a bacterium filtration efficiency detection device of gauze mask surface fabric, includes detection cabinet (1), aerial fog pipe (2), its characterized in that: a negative pressure test cavity (11) is arranged in the detection cabinet (1), a sealing movable door (12) is installed on the detection cabinet (1), the sealing movable door (12) can seal the negative pressure test cavity (11), the aerial fog pipe (2) is fixed in the middle of the negative pressure test cavity (11), a fog liquid generating device (3) is connected to the upper end pipeline of the aerial fog pipe (2), two sampling detection devices (4) are connected to the lower end pipeline of the aerial fog pipe (2), each sampling detection device (4) comprises a sampling device base (41) and drawer box mounting screw rods (42), the two drawer box mounting screw rods (42) are arranged in parallel and are vertically installed on the sampling device base (41), a plurality of drawer box assemblies (43) are stacked on the two drawer box mounting screw rods (42), and a pressing spiral plate (44) is installed at the upper end of each drawer box mounting screw rod (42), the pressing screw disc (44) can be used for laminating and pressing a plurality of drawer dish box assemblies (43).
2. The mask fabric bacteria filtration efficiency detection device according to claim 1, wherein: two operating gloves (121) are installed on the sealing movable door (12), and an observation window (122) is arranged on the sealing movable door (12).
3. The mask fabric bacteria filtration efficiency detection device according to claim 1, wherein: the fog liquid generating device (3) comprises a vibration liquid mixing mechanism (31), a peristaltic pump (32) and an atomizer (33), the vibration liquid mixing mechanism (31) comprises a test tube box (311), a bacteria liquid test tube (312), a test tube box chassis (313) and a test tube box vibration driving component (314), the outer side wall of the test tube box (311) is symmetrically connected with a test tube box moving wing (3111), a plurality of bacteria liquid test tubes (312) are inserted on the test tube box (311), the test tube box chassis (313) is fixedly installed on the inner wall of a negative pressure test cavity (11), two symmetrically arranged test tube box moving grooves (3131) are formed in the test tube box chassis (313), the size of each test tube box moving groove (3131) is matched with the test tube box moving wing (3111), the test tube box (311) is movably installed on the test tube box chassis (313) through the test tube box moving wing (3111), and the bottom of the test tube box (311) is connected with two impact rods (3112), the test tube box comprises a test tube box underframe (313), wherein a striking rod moving groove (3132) is formed in the test tube box underframe (313), a test tube box (311) is installed, the striking rod (3112) is inserted into the striking rod moving groove (3132), a test tube box oscillation driving assembly (314) comprises a motor (3141) and a cam (3142), the motor (3141) is fixedly installed on the inner wall of a negative pressure test cavity (11), the cam (3142) is fixed on an output shaft of the motor (3141), the cam (3142) is arranged between two striking rods (3112), the two striking rods (3112) are arranged on a motion track line of the cam (3142), the input end of a peristaltic pump (32) is connected with a test tube (312) pipeline, a bacteria liquid inlet (331), a spray outlet (332) and a bacteria liquid residual liquid recycling port (333) are formed in a sprayer (33), and the output end of the peristaltic pump (32) is connected with the bacteria liquid inlet (331) pipeline, the residual liquid recycling port (333) is connected with a bacteria liquid test tube (312) through a pipeline, a mist inlet (21) is formed in the top of the mist tube (2), and the mist inlet (21) is connected with a mist outlet (332) through a pipeline.
4. The mask fabric bacteria filtration efficiency detection device according to claim 3, wherein: aerial fog pipe (2) top is equipped with auxiliary air current import (22), two auxiliary air current import (22) branch is listed as fog liquid import (21) both sides, aerial fog pipe (2) middle part is equipped with pressure detection interface (23), aerial fog pipe (2) bottom is equipped with two symmetrical arrangement's fog liquid export (24), every fog liquid export (24) all pipe connection is to one set of sampling detection device (4) on.
5. The mask fabric bacteria filtration efficiency detection device according to claim 1, wherein: the drawer-type dish box assembly (43) comprises a drawer-type dish box base (431), a drawer-type dish box (432) and a box pressing cover (433), two drawer-type dish box moving groove rails (4311) which are symmetrically arranged are arranged on the drawer-type dish box base (431), a box body rear limiting seat (4312) is connected behind the drawer-type dish box moving groove rails (4311), drawer-type dish box moving wings (4321) are symmetrically connected to the outer wall of the drawer-type dish box (432), the size and the installation position of each drawer-type dish box moving wing (4321) are matched with the drawer-type dish box moving groove rails (4311), the drawer-type dish box (432) is movably inserted into the drawer-type dish box base (431) through the drawer-type dish box moving wings (4321), a closing groove (4313) is formed in the rear limiting seat (4312), two drawer-type dish box moving grooves (4311) are connected to the connection column (4314) in the side face to face, and bottom of the connection column (4314) are provided with a spring hole (43141) penetrating through the drawer-type dish connection column (4314), the spring hole (43141) is internally provided with a spring (4315), the box pressing cover (433) comprises a pressing cover plate (4331), the lower end face of the pressing cover plate (4331) is connected with a circle of pressing cloth flange (4332) and a circle of pressing cloth flange (4332) with the size matched with the box opening of the drawer box (432), the front end of the pressing cover plate (4331) is connected with a drawer baffle plate (4333), the rear end of the pressing cover plate (4331) is connected with a cover closing inserting plate (4334), the size of the cover closing inserting plate (4334) is matched with a cover closing groove (4313), the two sides of the pressing cover plate (4331) are connected with connecting lugs (4335) which are symmetrically arranged, the lower end of the connecting lug (4335) is connected with a guide column (43361), a stud inserting hole (4336) is arranged in the guide column (4336), the stud inserting hole (43361) runs through the guide column (4336) and the connecting lug (4335), the diameter of the stud inserting hole (43361) is matched with the inserting hole (42), the guide post (4336) is matched with the spring hole (43141) in outer diameter, a handle (4322) is installed on the front outer wall of the drawer box (432), and a first sealing rubber strip (4323) is installed on the upper end face of the side wall of the drawer box (432).
6. The mask fabric bacteria filtration efficiency detection device according to claim 5, wherein: a mist passing area (4325) is arranged at the lower bottom of the drawer dish box (432) of the top layer and the middle layer, a plurality of small through holes (43251) are arranged in the mist passing area (4325), second sealing rubber strips (4324) are arranged on the bottom end face of the drawer dish box (432), the second sealing rubber strips (4324) are arranged at the periphery of the mist passing area (4325) in a surrounding manner, mist passing holes (4337) are formed in the box pressing covers (433) of the middle layer and the bottom layer, the area of each mist passing hole (4337) is larger than that of the mist passing area (4325), the area of each mist passing hole (4337) is smaller than that of the second sealing rubber strip (4324), a sampling mist inlet interface (4338) is connected to the box pressing cover (433) of the top layer, a surface material rack mounting table (434) is mounted in the drawer dish box (432) of the top layer, a surface material laying frame (435) is fixedly mounted on the surface material laying frame (435), and an exhaust port (436) is connected to the side wall of the drawer dish box (432) at the bottom layer.
7. A method for detecting the bacterial filtration efficiency of mask fabric is characterized by comprising the following steps: the method comprises the following steps:
1) sequentially stacking a plurality of drawer-type dish box assemblies (43) on a sampling device base (41) to form a sampling detection device (4), loading cut mask fabric to be detected into the drawer-type dish box assembly (43) at the top layer, and pressing the plurality of drawer-type dish box assemblies (43) by rotating a pressing screw disc (44);
2) inserting a plurality of bacteria liquid test tubes (312) on the test tube box (311), and making connecting tubes of all pipelines;
3) the test tube box is started to vibrate the driving assembly (314) to drive the bacteria liquid test tube (312) to vibrate left and right to mix liquid, then the peristaltic pump (32) and the sprayer (33) are started, the bacteria liquid is formed into fog-shaped aerosol to be introduced into the aerosol pipe (2), and the fog pipe (2) is used for conveying the aerosol to the sampling detection device (4);
4) after experiment a period of time, each equipment in step 3) is closed, after a period of time of stewing, upwards twist and compress tightly spiral shell dish (44), remove the state of compressing tightly of steamer tray dish subassembly (43), extract one or more steamer tray dish boxes (432) and go to carry out the bacterial culture, observe, gather bacterial colony data.
CN202110174586.3A 2021-02-07 2021-02-07 Bacteria filtering efficiency detection device and detection method for mask fabric Active CN113008754B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110174586.3A CN113008754B (en) 2021-02-07 2021-02-07 Bacteria filtering efficiency detection device and detection method for mask fabric

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110174586.3A CN113008754B (en) 2021-02-07 2021-02-07 Bacteria filtering efficiency detection device and detection method for mask fabric

Publications (2)

Publication Number Publication Date
CN113008754A true CN113008754A (en) 2021-06-22
CN113008754B CN113008754B (en) 2023-08-25

Family

ID=76384003

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110174586.3A Active CN113008754B (en) 2021-02-07 2021-02-07 Bacteria filtering efficiency detection device and detection method for mask fabric

Country Status (1)

Country Link
CN (1) CN113008754B (en)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002098704A (en) * 2000-09-25 2002-04-05 Olympus Optical Co Ltd Autoanalyzer of bacteria
CN101629892A (en) * 2009-08-20 2010-01-20 青岛众瑞智能仪器有限公司 Filtering efficiency detector of air filter materials
CN103999755A (en) * 2014-05-26 2014-08-27 魏延恕 Anti-drop type soilless culture system with electric interlocking device
KR101721190B1 (en) * 2015-11-09 2017-03-30 재단법인경북테크노파크 Microorganism Filter Test System
CN206680506U (en) * 2017-04-17 2017-11-28 新乡医学院 A kind of cell culture case
CN206927895U (en) * 2017-04-07 2018-01-26 安徽九川生物科技有限公司 A kind of sheep interferon target gene screening reagent box
CN208200932U (en) * 2018-04-16 2018-12-07 马吉云 A kind of portable microculture box
CN209739660U (en) * 2019-04-03 2019-12-06 訾利芳 Tool storage box
CN210875099U (en) * 2019-08-11 2020-06-30 天津九鼎鑫星生物科技有限公司 Medicine vibrating device for clinical experiment
CN211785014U (en) * 2020-04-03 2020-10-27 上海简户仪器设备有限公司 Stable bacterial filtration test equipment
CN211927635U (en) * 2020-03-31 2020-11-13 青岛众瑞智能仪器有限公司 Comprehensive detection device for medical mask

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002098704A (en) * 2000-09-25 2002-04-05 Olympus Optical Co Ltd Autoanalyzer of bacteria
CN101629892A (en) * 2009-08-20 2010-01-20 青岛众瑞智能仪器有限公司 Filtering efficiency detector of air filter materials
CN103999755A (en) * 2014-05-26 2014-08-27 魏延恕 Anti-drop type soilless culture system with electric interlocking device
KR101721190B1 (en) * 2015-11-09 2017-03-30 재단법인경북테크노파크 Microorganism Filter Test System
CN206927895U (en) * 2017-04-07 2018-01-26 安徽九川生物科技有限公司 A kind of sheep interferon target gene screening reagent box
CN206680506U (en) * 2017-04-17 2017-11-28 新乡医学院 A kind of cell culture case
CN208200932U (en) * 2018-04-16 2018-12-07 马吉云 A kind of portable microculture box
CN209739660U (en) * 2019-04-03 2019-12-06 訾利芳 Tool storage box
CN210875099U (en) * 2019-08-11 2020-06-30 天津九鼎鑫星生物科技有限公司 Medicine vibrating device for clinical experiment
CN211927635U (en) * 2020-03-31 2020-11-13 青岛众瑞智能仪器有限公司 Comprehensive detection device for medical mask
CN211785014U (en) * 2020-04-03 2020-10-27 上海简户仪器设备有限公司 Stable bacterial filtration test equipment

Also Published As

Publication number Publication date
CN113008754B (en) 2023-08-25

Similar Documents

Publication Publication Date Title
CN105880017A (en) Bi-directional dust collection system
CN102095835A (en) Device for simulating coal and gas outburst experiment
CN206640980U (en) Aspirated-air type high density precision drill
CN113008754A (en) Device and method for detecting bacteria filtering efficiency of mask fabric
CN201935906U (en) Simulator for coal and gas outburst tests
CN209900902U (en) Air extractor for laboratory
CN209061254U (en) A kind of chemical industry grinding device
CN104370579B (en) A kind of glaze spraying device
CN208466555U (en) Color selector and splicing dust exhaust apparatus for color selector
CN108176148A (en) A kind of circulation type powder filter device and filter method based on Bernoulli effect
CN209106571U (en) A kind of medical product storage medical cupboard
CN210559705U (en) Oil-water separation device for oily sewage
CN205007755U (en) Unpowered deashing bag dust collector
CN207917345U (en) A kind of automatic Unpacking of cleaning-type
CN207045861U (en) A kind of sterilization filling nozzle for soya sauce
CN206103476U (en) Filter
CN214571917U (en) Spore trapping instrument
CN203997370U (en) A kind of milk powder preservation device
CN217888642U (en) Edulcoration device is used in herbal pieces-production
CN219104838U (en) Liquid chromatograph mobile phase stock solution taking device
CN209717163U (en) A kind of automation drilling machine dust collecting
CN216639745U (en) Based on duck fine hair goose fine hair product is with dividing hair equipment
CN209206005U (en) A kind of biologic cleanliness safety cabinet being conveniently replaceable filter
CN216464028U (en) Vacuum dust removal type shot blasting machine
CN221628515U (en) Cabinet type centrifugal fan

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant