CN112986465A - Multilayer assembling preparation method of chromatographic analysis column - Google Patents
Multilayer assembling preparation method of chromatographic analysis column Download PDFInfo
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- CN112986465A CN112986465A CN202110204380.0A CN202110204380A CN112986465A CN 112986465 A CN112986465 A CN 112986465A CN 202110204380 A CN202110204380 A CN 202110204380A CN 112986465 A CN112986465 A CN 112986465A
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- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000000945 filler Substances 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000003825 pressing Methods 0.000 claims abstract description 19
- 238000006073 displacement reaction Methods 0.000 claims abstract description 10
- 238000012856 packing Methods 0.000 claims description 13
- 239000000725 suspension Substances 0.000 claims description 11
- 239000006185 dispersion Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 238000002525 ultrasonication Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 12
- 238000011049 filling Methods 0.000 abstract description 11
- 238000005429 filling process Methods 0.000 abstract description 4
- 238000004220 aggregation Methods 0.000 abstract description 3
- 230000002776 aggregation Effects 0.000 abstract description 3
- 239000002245 particle Substances 0.000 abstract description 3
- 238000004062 sedimentation Methods 0.000 abstract description 3
- 239000004793 Polystyrene Substances 0.000 description 11
- 229920002223 polystyrene Polymers 0.000 description 11
- 230000000694 effects Effects 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical group CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 125000001997 phenyl group Chemical class [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- 238000004237 preparative chromatography Methods 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000010829 isocratic elution Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/56—Packing methods or coating methods
- G01N2030/562—Packing methods or coating methods packing
- G01N2030/565—Packing methods or coating methods packing slurry packing
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
A multilayer assembly preparation method of a chromatographic column relates to the preparation of chromatographic columns. Connecting a hollow column pipe with a sieve plate and a fixed nut at the outlet end with a homogenate tank, dividing the required filler homogenate into a plurality of equal parts, pouring the equal parts into the homogenate tank in sequence, pressing the displacement liquid into the homogenate tank through a high-pressure pump, further pressing the chromatographic filler into the hollow column pipe in sequence, pressing all the equal parts of the homogenate into the chromatographic column pipe, taking down the chromatographic column, and adding the sieve plate and the fixed nut to obtain the chromatographic analysis column prepared by multilayer assembly. The technology can be used for filling chromatographic columns with more compact column beds, and the obtained chromatographic columns have the advantages of high column efficiency and the like. Because the multilayer assembly method is adopted, when each layer of column bed is filled, filler particles in the homogenate liquid are in a highly dispersed state, the aggregation and sedimentation of the filler in the conventional single filling process are avoided, and the existence of column bed unevenness and gaps is reduced. The method is simple to operate, and can remarkably improve the separation performance of the chromatographic column.
Description
Technical Field
The invention relates to preparation of a chromatographic column, in particular to a multilayer assembly preparation method of a chromatographic analysis column.
Background
The uniformity of a chromatographic column bed is a decisive factor influencing the column efficiency, and factors such as the type of homogenate, the type of displacement liquid, column loading pressure and the like of chromatographic packing are considered and optimized in order to improve the column efficiency, and the final aim is to obtain a compact and uniform column bed. In the traditional filling process, filler homogenate is poured into a homogenate tank with a larger volume at one time, the process that the homogenate with the large volume is replaced by hydraulic pressure and is fed into a chromatographic column tube is a longer process, generally 10-20 min, in the process, adverse phenomena such as aggregation, sedimentation and the like gradually occur in the filler, and further gaps with different degrees appear in a column bed in the chromatographic column tube, and non-uniformity occurs in the column bed at the front end and the rear end of the chromatographic column, and the column efficiency is reduced.
Disclosure of Invention
The invention aims to overcome the defects of the conventional chromatographic column filling method, and provides a multilayer assembly preparation method of a chromatographic column, which can realize the highly uniform filling preparation of a chromatographic column bed, obviously improve the column efficiency of the chromatographic column, obtain a chromatographic column with a highly uniform bed and obtain a high-column-efficiency chromatographic column.
The method comprises the following specific steps: connecting a hollow column pipe with a sieve plate and a fixed nut at the outlet end with a homogenate tank, dividing the required filler homogenate into a plurality of equal parts, pouring the equal parts into the homogenate tank in sequence, pressing the displacement liquid into the homogenate tank through a high-pressure pump, further pressing the chromatographic filler into the hollow column pipe, pressing all the equal parts of the homogenate into the chromatographic column pipe, taking down the chromatographic column, and adding the sieve plate and the fixed nut to obtain the multilayer assembled chromatographic analysis column.
Dividing the required filler homogenate into a plurality of equal parts, namely, mixing the filler required by filling one chromatographic column with the corresponding dispersion liquid to form a filler suspension, and then dividing the filler suspension into a plurality of equal parts, preferably five equal parts; the dispersion and suspension of the filler is maintained by stirring, ultrasonication, etc. prior to pouring into the surge tank.
The specific method for pressing the chromatographic packing into the hollow column tube can be as follows: and (3) opening the homogenate tank after the suspension in the homogenate tank is completely pressed into the chromatographic column tube each time, taking out the displacement liquid in the homogenate tank, pouring the next homogenate liquid, sealing, continuously pressing the filler into the chromatographic column tube, and repeating the operation until all the homogenate liquid is pressed into the chromatographic column tube.
The pressure of pressing into the chromatographic column tube can be 2000-15000 psi.
After all equal parts of the homogenate are pressed into the chromatographic column tube, the filler in the chromatographic column tube can be further continuously compacted by adopting higher pressure, the pressure is maintained for 10-60 min, then the pressure is gradually reduced, and the lowest pressure is maintained for 30-60 min, so that the pressure in the column bed is fully balanced.
The high pressure is higher than the pressure of pressing in the homogenate liquid, and the low pressure is the lowest pressure of the column filling system.
The step of taking down the chromatographic column and adding the sieve plate and the fixing screw cap is to separate the chromatographic column from the lower end of the homogenate tank, and the sieve plate and the fixing screw cap are added at the front end of the column tube.
The inner diameter of the chromatographic column tube can be 2-1000 mm, and the length can be 10-5000 mm.
The invention can be used for filling chromatographic columns with more compact column beds, and the obtained chromatographic columns have the advantages of high column efficiency and the like. Because a multilayer assembly method is adopted, when each layer of column bed is filled, the homogenate liquid is highly dispersed chromatographic packing, thereby avoiding the aggregation and sedimentation of the packing in the conventional single filling process and reducing the existence of column bed nonuniformity and gaps. The method is simple to operate, and can remarkably improve the separation performance of the chromatographic column.
Drawings
FIG. 1 is a diagram of a five-layer assembled device for preparing a porous polystyrene chromatographic column according to the present invention.
FIG. 2 is a diagram of a device for preparing a porous polystyrene chromatographic column by a conventional single homogenization filling method.
FIG. 3 is a diagram showing the separation effect of the preparative chromatography column according to the embodiment of the present invention.
FIG. 4 is a graph showing the separation effect of a column for comparative example preparative chromatography.
Detailed Description
The invention will be further described with reference to the drawings and several alternative embodiments. It is to be noted that: the present invention is not limited to the following examples. Any of the features and embodiments in the following examples are one or more of a variety of alternative features and alternative embodiments. For the sake of simplicity of description, this document is not exhaustive of all the alternative technical features and embodiments encompassed by the present invention. The examples do not show the specific techniques and conditions, and the reagents and apparatus are not shown in the manufacturers, and the reagents and apparatus are all conventional products commercially available, according to the techniques and conditions described in the literature in the field or according to the specifications of the products.
The embodiment of the invention comprises the following specific steps: connecting a hollow column pipe with a sieve plate and a fixed nut at the outlet end with a homogenate tank, dividing the required filler homogenate into a plurality of equal parts, pouring the equal parts into the homogenate tank in sequence, putting the displacement liquid into the homogenate tank through a high-pressure pump, pressing the chromatographic filler into the hollow column pipe, pressing all the equal parts of the homogenate into the chromatographic column pipe, taking down the chromatographic column, and adding the sieve plate and the fixed nut to obtain the chromatographic column.
Furthermore, the chromatographic column packing preparation system is a mainstream hydraulic or pneumatic packing system and comprises a high-pressure system, a homogenate tank, an empty column pipe and various sealing fittings.
Further, the filler required for filling one chromatographic column is mixed with the corresponding dispersion liquid to form a filler suspension, which is divided into several equal parts, preferably five equal parts, and the dispersion and suspension of the filler is maintained by stirring, ultrasound, etc. before being poured into the homogenization tank.
Further, after the suspension in the homogenate tank is completely pressed into the chromatographic column tube each time, the homogenate tank is opened, the displacement liquid in the homogenate tank is taken out, then the next homogenate is poured into the homogenate tank, the filler is continuously pressed into the chromatographic column tube after sealing, and the operation is repeated until all the filler homogenate is pressed into the chromatographic column tube.
Further, after all the filler homogenate liquid is pressed into the column tube, the filler in the chromatographic column tube is further continuously compacted by adopting high pressure for generally 30-60 min, then the pressure is gradually reduced, and the lowest pressure is maintained for 30-60 min, so that the pressure in the column bed is fully balanced.
And further, after the filling process is finished, separating the chromatographic column from the lower end of the homogenate tank, and adding a sieve plate and a fixing screw cap at the front end of the column tube to obtain the chromatographic column.
Furthermore, the column packing method is suitable for chromatographic columns with the inner diameter of 2-1000 mm and the length of 10-5000 mm.
Specific examples and comparative examples are given below.
Example (b): five-layer assembled porous polystyrene chromatographic column
As shown in fig. 1, an analytical column (3) of size 4.6 x 25mm was packed using a 20mL volume homogenizer tank (1) and a high pressure pump infusion system (2). 2g of porous cross-linked polystyrene chromatography packing, 5 μm in particle size, was mixed with 100mL of isopropanol, sonicated for 5min, and then aliquoted into five 50mL beakers (4), 20mL of homogenate per beaker. Connecting an analytical column (3) with a sieve plate and a fixed screw at the outlet end with a homogenizing tank (1), pouring 20mL of homogenate into the homogenizing tank, stirring and performing ultrasound for 5min before pouring the homogenate, pressing 20mL of homogenate into a chromatographic column tube under the pressure of 5000psi, then reducing the pressure to normal pressure, pouring out the displacement liquid in the homogenizing tank, adding the uniformly mixed 20mL of homogenate, continuing to load the column under the pressure of 5000psi, repeating the operation until 100mL of homogenate is pressed into the chromatographic column tube, then increasing the pressure to 7000psi for maintaining for 30min, then reducing the pressure to 0psi, maintaining for 30min, separating the chromatographic column from the lower end of the homogenizing tank, adding a sieve plate and a fixed screw cap at the front end of the column tube, and obtaining the five-layer assembled polystyrene chromatographic column (3).
Comparative example: preparation of porous polystyrene chromatographic column by traditional single homogenate filling method
As shown in fig. 2, the homogenate tank was replaced with a 100mL volume homogenate tank (5) by using the same hydraulic column packing system as in example 1, and an analytical column (6) having a size of 4.6 × 25mm was packed. Weighing 2g of porous crosslinked polystyrene chromatographic filler with the particle size of 5 microns, mixing the chromatographic filler with 100mL of isopropanol (7), carrying out ultrasonic treatment for 5min, pouring 100mL of homogenate into a homogenate tank (5), using ethanol as a displacement liquid, pressing 100mL of homogenate into a chromatographic column tube under the pressure of 5000psi, then reducing the pressure to 7000psi for 30min, then reducing the pressure to 0psi, maintaining for 30min, separating the chromatographic column from the lower end of the homogenate tank, and adding a sieve plate and a fixed screw cap at the front end of the column tube to obtain the polystyrene chromatographic column (6) prepared by a single homogenate filling method.
Column efficiency evaluation was performed on the columns prepared in examples and comparative examples:
the two columns prepared in examples and comparative examples were subjected to column efficiency evaluation, and the column efficiency and separation ability of the columns were evaluated.
The mobile phase A is water, the mobile phase B is acetonitrile, and the separated sample is a benzene series. The separation condition is 85% acetonitrile solution, the sample amount is 5 muL, the column temperature is 25 ℃, the flow rate is 1mL/min, the detection wavelength is 214nm, the separation is carried out by adopting an isocratic elution mode, the separation effect and the column effect of the porous polystyrene chromatographic column prepared by five-layer assembly are shown in table 1 and figure 3, and the separation effect and the column effect of the porous polystyrene chromatographic column prepared by the traditional single homogenate filling method are shown in table 2 and figure 4. As can be seen from the number of the benzene series plates, the porous polystyrene chromatographic column prepared by adopting the five-layer assembly has higher column efficiency.
The separation effect data of the chromatographic column prepared by the embodiment of the invention are shown in the table 1.
TABLE 1
Comparative example preparative chromatographic columns the separation performance data are shown in table 2.
TABLE 2
Experiments show that the chromatographic packing homogenate is divided into a plurality of times and is respectively injected into the homogenate tank and pressed into the chromatographic column tube in a plurality of times, and a column bed is formed in a multi-layer assembly mode. Each layer of filler is in a compact and non-porous state, and a uniform and perfect column bed is finally formed through multilayer assembly, so that the separation efficiency of the chromatographic column can be remarkably improved.
Claims (8)
1. A multilayer assembly preparation method of a chromatographic column is characterized by comprising the following specific steps: connecting a hollow column pipe with a sieve plate and a fixed nut at the outlet end with a homogenate tank, dividing the required filler homogenate into a plurality of equal parts, pouring the equal parts into the homogenate tank in sequence, pressing the displacement liquid into the homogenate tank through a high-pressure pump, further pressing the chromatographic filler into the hollow column pipe, pressing all the equal parts of the homogenate into the chromatographic column pipe, taking down the chromatographic column, and adding the sieve plate and the fixed nut to obtain the multilayer assembled chromatographic analysis column.
2. The method of claim 1, wherein the dividing of the desired filler homogenate into equal portions is performed by mixing the desired filler for packing one chromatography column with the corresponding dispersion to form a filler suspension, and then dividing the filler suspension into equal portions, preferably into five equal portions; the dispersion and suspension of the filler is maintained by stirring, ultrasonication, etc. prior to pouring into the surge tank.
3. The method for preparing a multilayer assembly of chromatographic analytical columns according to claim 1, wherein the specific method for pressing chromatographic packing into the hollow column tube is as follows: and (3) opening the homogenate tank after the suspension in the homogenate tank is completely pressed into the chromatographic column tube each time, taking out the displacement liquid in the homogenate tank, pouring the next homogenate liquid, sealing, continuously pressing the filler into the chromatographic column tube, and repeating the operation until all the homogenate liquid is pressed into the chromatographic column tube.
4. The method of claim 3, wherein the pressure applied to the column tube is 2000-15000 psi.
5. The method of claim 1, wherein after all the aliquots of the homogenate are pressed into the column tube, the packing material in the column tube is further compacted continuously at a higher pressure for 10-60 min, then gradually depressurized, and maintained at a minimum pressure for 30-60 min to fully equalize the pressure in the column bed.
6. The method of claim 5, wherein the higher pressure is higher than the pressure of the pressurized homogenate and the lowest pressure is the lowest pressure of the system in which the column is installed.
7. The method of claim 1, wherein the removing the chromatography column and the adding the sieve plate and the fixing nut are performed by separating the chromatography column from the lower end of the homogenate tank and adding the sieve plate and the fixing nut to the front end of the column tube.
8. The method of claim 1, wherein the inner diameter of the chromatography column tube is 2-1000 mm and the length thereof is 10-5000 mm.
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| CN202110204380.0A CN112986465A (en) | 2021-02-23 | 2021-02-23 | Multilayer assembling preparation method of chromatographic analysis column |
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| CN202110204380.0A CN112986465A (en) | 2021-02-23 | 2021-02-23 | Multilayer assembling preparation method of chromatographic analysis column |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078602A (en) * | 2022-05-30 | 2022-09-20 | 郑州大学第一附属医院 | Capillary high-pressure liquid chromatographic column and ultrasonic preparation method and application thereof |
| CN116351399A (en) * | 2022-12-30 | 2023-06-30 | 厦门色谱分析仪器有限公司 | Bonding cellulose derivative chiral liquid chromatographic column and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465975A (en) * | 2002-07-04 | 2004-01-07 | 中国科学院大连化学物理研究所 | Method for filling soil chromatographic column |
| JP2006078231A (en) * | 2004-09-07 | 2006-03-23 | Kurita Water Ind Ltd | Manufacturing method of chromatographic column |
| CN102989198A (en) * | 2012-11-27 | 2013-03-27 | 张勇 | Dry-method column-packing method for preparative columns |
| CN103675169A (en) * | 2012-08-31 | 2014-03-26 | 月旭材料科技(上海)有限公司 | Method for filling gel chromatographic columns |
| CN109030689A (en) * | 2018-07-17 | 2018-12-18 | 王晓飞 | The pre-assembled preparation method of capillary chromatographic column |
| CN209043854U (en) * | 2018-07-17 | 2019-06-28 | 晋江精纯科技有限公司 | A kind of pre-assembled capillary chromatographic column |
| CN111089924A (en) * | 2019-12-21 | 2020-05-01 | 江苏奥迪康医学科技股份有限公司 | Preparation process of glycosylated hemoglobin high-pressure liquid chromatography column |
-
2021
- 2021-02-23 CN CN202110204380.0A patent/CN112986465A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465975A (en) * | 2002-07-04 | 2004-01-07 | 中国科学院大连化学物理研究所 | Method for filling soil chromatographic column |
| JP2006078231A (en) * | 2004-09-07 | 2006-03-23 | Kurita Water Ind Ltd | Manufacturing method of chromatographic column |
| CN103675169A (en) * | 2012-08-31 | 2014-03-26 | 月旭材料科技(上海)有限公司 | Method for filling gel chromatographic columns |
| CN102989198A (en) * | 2012-11-27 | 2013-03-27 | 张勇 | Dry-method column-packing method for preparative columns |
| CN109030689A (en) * | 2018-07-17 | 2018-12-18 | 王晓飞 | The pre-assembled preparation method of capillary chromatographic column |
| CN209043854U (en) * | 2018-07-17 | 2019-06-28 | 晋江精纯科技有限公司 | A kind of pre-assembled capillary chromatographic column |
| CN111089924A (en) * | 2019-12-21 | 2020-05-01 | 江苏奥迪康医学科技股份有限公司 | Preparation process of glycosylated hemoglobin high-pressure liquid chromatography column |
Non-Patent Citations (3)
| Title |
|---|
| 冯国政: "液相色谱柱高压匀浆填充装置", 《分析仪器》 * |
| 刘国诠 等: "《色谱柱技术》", 31 July 2001, 北京：化学工业出版社 * |
| 赵继红 等: "高效液相色谱柱的稀匀浆填充方法", 《北方工业大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078602A (en) * | 2022-05-30 | 2022-09-20 | 郑州大学第一附属医院 | Capillary high-pressure liquid chromatographic column and ultrasonic preparation method and application thereof |
| CN115078602B (en) * | 2022-05-30 | 2024-05-28 | 郑州大学第一附属医院 | Capillary high-pressure liquid chromatographic column and ultrasonic preparation method and application thereof |
| CN116351399A (en) * | 2022-12-30 | 2023-06-30 | 厦门色谱分析仪器有限公司 | Bonding cellulose derivative chiral liquid chromatographic column and preparation method and application thereof |
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