CN112986228B - Hydrogel with uric acid detection function and application of hydrogel to disposable nursing products - Google Patents
Hydrogel with uric acid detection function and application of hydrogel to disposable nursing products Download PDFInfo
- Publication number
- CN112986228B CN112986228B CN202110225080.0A CN202110225080A CN112986228B CN 112986228 B CN112986228 B CN 112986228B CN 202110225080 A CN202110225080 A CN 202110225080A CN 112986228 B CN112986228 B CN 112986228B
- Authority
- CN
- China
- Prior art keywords
- hydrogel
- uric acid
- lutetium
- color
- bismuth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/07—Stiffening bandages
- A61L15/08—Stiffening bandages containing inorganic materials, e.g. plaster of Paris
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/07—Stiffening bandages
- A61L15/10—Stiffening bandages containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Materials Engineering (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Plasma & Fusion (AREA)
- Dispersion Chemistry (AREA)
- Immunology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The application relates to the field of disposable sanitary articles, in particular to application of hydrogel with uric acid detection function in the disposable sanitary articles. A hydrogel with uric acid detection function comprises ethylene glycol and a lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent coated with ligands on the surface, wherein the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent coated with ligands on the surface accounts for 7-13% of the whole hydrogel in percentage by mass. The hydrogel with uric acid detection function is especially suitable for users with high uric acid.
Description
Technical Field
The application relates to the field of disposable sanitary articles, in particular to application of hydrogel with uric acid detection function in the disposable sanitary articles.
Background
The disposable nursing pad has high use rate in bedridden nursing, light incontinence nursing and rehabilitation nursing, and the structure of the disposable nursing pad comprises a leakage-proof bottom film, an absorption core body and a surface layer, wherein the absorption core body is positioned between the leakage-proof bottom film and the surface layer. When in use, the nursing pad is flatly laid on the buttocks and the lower part of the user, so as to prevent the urine discharged by the patient from polluting the bed sheet. The existing nursing pad has only a simple antifouling function and has no uric acid detection function.
The main components of human urine comprise water, inorganic salts, organic matters and the like, wherein the inorganic matters contain uric acid components. The quality of the uric acid newly generated in the normal human body is equivalent to that of the excreted uric acid, and the uric acid is in a dynamic balance state, if excessive uric acid is generated in the human body and can not be excreted in time or the uric acid excreting mechanism is degenerated, the excessive in-vivo uric acid can be remained, when the concentration of the uric acid is high, the body fluid of the human body becomes acidic, the normal functions of human cells are affected, and gout can be caused seriously. The uric acid determination method mainly comprises a phosphotungstic acid reduction method, a uricase-peroxidase coupling method, a uric acid sensor detection method, a voltammetry method and the like [1-4], and the methods are used with specific instruments, so that real-time visual judgment is difficult to realize.
As is known from literature studies, uric acid has a molecular formula of C 5 H 4 N 4 O 3 The molecular structure of the kit contains a plurality of-NH bonds, and the color developing agent directly shows color change through reaction with substances, but the common color developing agent cannot directly react with uric acid, so that the novel color developing agent capable of reacting with uric acid is developed, the accuracy of uric acid detection can be improved, the detection method is simplified, and the kit has good application prospect in the field of multifunctional development of disposable nursing pads.
Reference to the literature
[1] A. B. Patil, C. B. Zheng, L. Y. Ma, R. H. Wu, S. K. Mengane, Y. F. Zhang, X. T. Liu, Z. H. Meng, W. L. Zhang, Z. J. Xu, C. F. Chen, J. N. Huang, X. Y. Liu, Nanotechnology, 2021, 32, 065502.
[2] C. Y. Huang, H. C. Hsiao, Sensors, 2020, 20.
[3] C. Borghi, C. Bentivenga, E. R. Cosentino, International Journal of Cardiology, 2020, 320, 23-24.
[4] H. Y. Zhang, S. Liu, Journal of Alloys and Compounds, 2020, 842, 155873。
Disclosure of Invention
In order to enrich the functions of disposable nursing products, the application aims to provide the hydrogel with the uric acid detection function, after the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent with the surface coated with the ligand in the hydrogel encounters urine, hydroxyl (-OH) on the surface of the compound and-NH in urine molecules form bonds through electrostatic attraction, and then bismuth lutetium potassium iodide in the compound and uric acid in the urine act to form an organic-inorganic complex, so that the complex presents deep yellow color and can be well applied to quantitative concentration detection of uric acid.
In order to achieve the above purpose, the present application adopts the following technical scheme:
a hydrogel with uric acid detection function comprises ethylene glycol and a lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent coated with ligands on the surface, wherein the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent coated with ligands on the surface accounts for 7-13% of the whole hydrogel in percentage by mass.
Preferably, the ligand coated on the surface of the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developer is citric acid and EDTA. The molar ratio of citric acid to EDTA is 1: (0.7-0.9). The molecular formula of the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent is as follows: KLuF (kluF) 4 @KLu 0.2 Bi 0.8 I 4 。
Preferably, the hydrogel absorbs 5-50 milliliters of urine, the hydrogel does not develop color when the uric acid concentration in the urine is lower than a threshold value, the hydrogel shows yellow when the uric acid concentration in the urine is higher than the threshold value, and the uric acid concentration is in direct proportion to the brightness of the color developer. The threshold value is that the uric acid concentration in urine is 420 mu mol/L.
Preferably, the hydrogel is prepared from deionized water, polyethylene glycol diacetate acrylate, ethylene glycol, 2-hydroxy-2-methyl-1- [4- (2-hydroxyethoxy) phenyl ] -1-propanone and a color developing agent of a complex of potassium lutetium fluoride-bismuth lutetium iodide coated with a ligand on the surface.
Preferably, the preparation method of the hydrogel with uric acid detection function comprises (1) dissolving prepared citric acid and ethylenediamine tetraacetic acid ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent (50 mg) in (5-20) ml deionized water, then adding acrylic acid polyethylene glycol diester (10-15) ml (2) to carry out ultrasonic treatment for 5-12 hours in an ultrasonic instrument with the power of more than 1KW, then adding 2-hydroxy-2-methyl-1- [4- (2-hydroxyethoxy) phenyl ] -1-acetone, and stirring for 20-40 minutes at room temperature; (3) Irradiating the solution with laser with power of 1-5W and wavelength of 659 nm for 15-30 min, and adding 50 microliter of ethylene glycol every 1 min during irradiation to obtain hydrogel with porous structure.
The disposable nursing article has the hydrogel with uric acid detecting function.
The disposable nursing pad set comprises a packaging bag, a color rendering index card and a plurality of pieces of disposable nursing articles; the brightness of the color displayed by the hydrogel is changed into a color rendering index through the value of the fluorescence intensity, and the color rendering index card corresponds to the color rendering index of the hydrogel.
Further, the application provides a preparation method of the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent, which comprises the following steps:
1) Dissolving (1-2) millimoles lutetium acetate, (1-2) millimoles potassium acetate and (4-8) millimoles ammonium fluoride in (4-8) milliliters of deionized water, and stirring (30-60) for minutes at room temperature, wherein the molar ratio of lutetium acetate, potassium acetate and ammonium fluoride is 1:1:4;
2) Adding (20-30) ml oleic acid and (10-20) ml octadecene into 1), stirring (30-60) at room temperature, transferring into a hydrothermal kettle, and adding into a solvent under stirring at 160% o C-200 o C, preserving heat (12-24 hours);
3) Centrifugally washing the product obtained in the step 2) for (3-5) times by using a mixed solution of cyclohexane and ethanol (volume ratio is 1:3), and then dispersing the obtained nanocrystalline in (4-8) milliliters of cyclohexane;
4) Adding 1-2 ml of dilute hydrochloric acid (1.6 mol/L) into the solution obtained in the step 3), performing ultrasonic treatment for 30-60 minutes in an ultrasonic cleaner with the power of more than 1 kilowatt, then performing centrifugal washing (1-2) for times by using a mixed solution of ethanol and deionized water (the volume ratio is 1:3), and dispersing the obtained nanocrystalline in (2-4) ml of deionized water;
5) Stirring basic bismuth nitrate (0.5-1) millimole, lutetium acetate (0.33-0.66) millimole, glacial acetic acid (5-20) ml and deionized water (40-60) ml for 30-60 minutes at room temperature, wherein the molar ratio of bismuth nitrate to lutetium acetate is 3:2;
6) Adding the nanocrystalline solution obtained in the step (4) into the solution obtained in the step (5), and stirring for 30-60 minutes at room temperature;
7) Adding potassium iodide (the molar quantity of which is 4/5 of that of bismuth nitrate) and dilute hydrochloric acid (0.3-1) ml into the solution obtained in the step 6), and stirring (10-20) for minutes at room temperature;
8) Adding citric acid and EDTA (the molar ratio of which is 1: (0.7-0.9) in an amount of 1.5 times the molar amount of potassium iodide in the chamberStirring at a temperature for 2-5 min, and then placing at 30-40 o And C, standing for 24-48 hours in the oven of the formula, and finally storing the obtained color developing agent in a black bottle.
After the hydrogel adsorbs urine, the hydrogel dissolves, the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent coated with the ligand on the surface in the hydrogel contacts uric acid, whether the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent is displayed or not is determined by detecting the amount of uric acid, namely the uric acid concentration in the urine is lower than a threshold value, the hydrogel does not develop color, when the uric acid concentration in the urine is higher than the threshold value, the hydrogel shows yellow, the brightness degree of the hydrogel is in direct proportion to the uric acid concentration, the brightness degree of the hydrogel gradually deepens along with the increase of the uric acid concentration, and after the uric acid concentration reaches a certain degree, EDTA can adsorb enough sodium ions, and the color-developing agent is changed into orange. In conclusion, the hydrogel with uric acid detection function is particularly suitable for users with high uric acid.
Drawings
FIG. 1 shows the results of an inductively coupled plasma atomic emission spectrometry analysis of a potassium lutetium fluoride-bismuth lutetium iodide color developer.
FIG. 2 is a transmission electron microscope image of a potassium lutetium fluoride-bismuth lutetium iodide complex developer.
FIG. 3 yellow light intensity versus uric acid concentration for citric acid and EDTA dual ligand coated potassium lutetium fluoride-bismuth lutetium iodide.
FIG. 4 is a graph of yellow light intensity versus uric acid concentration for a color developer without surface ligand modification.
FIG. 5 yellow intensity versus uric acid concentration for a citric acid ligand-modified developer alone.
FIG. 6 yellow intensity versus uric acid concentration for EDTA ligand-modified color developers alone.
FIG. 7 is a graph showing uric acid concentration detection performance of hydrogels.
Detailed Description
Experimental part
The main reagent comprises: potassium acetate (99.0%), ammonium fluoride (99.0%), bismuth subnitrate (98%), lutetium acetate (99.9%), potassium iodide (99.0%), glacial acetic acid (100%), oleic acid, octadecene (90%) and uric acid (99%) were purchased from Sigma-Aldrich, cyclohexane, dilute hydrochloric acid and deionized water were purchased from national pharmaceutical chemicals limited, and test urine was taken from adults.
Characterization instrument and method
Inductively coupled plasma atomic emission spectroscopy (ZX-Z5000-WLD 5000, powder sample, frequency 50/60 Hz), transmission electron microscopy (TEM, FEI Tecnai G2F 20), spectrometer (FLUROHUB-B, HORIBA JOBIN YVON).
Preparation of atomic emission spectrometry test samples: freezing and drying the color developing agent to obtain a powdery sample;
preparation of a transmission electron microscope sample: a little color reagent is taken and dissolved in 4 ml of ethanol solution, and 3 to 6 drops of liquid are dropped on the ultrathin carbon film after 5 minutes of ultrasound.
The uric acid detection method comprises the following steps: removing a certain volume of color developing agent, dividing into a plurality of groups, adding uric acid with different molar amounts into each group, characterizing the change of fluorescence intensity by a fluorescence spectrometer, and fitting a standard curve.
The concentration of uric acid refers to the concentration of uric acid per unit volume of aqueous solution.
KLuF 4 @KLu 0.2 Bi 0.8 I 4 Is prepared from
1 millimole of lutetium acetate, 1 millimole of potassium acetate and 4 millimoles of ammonium fluoride are dissolved in 6 ml of deionized water and stirred at room temperature for 30 minutes; then 20 ml oleic acid and 10 ml octadecene were added, stirred at room temperature for 60 minutes, then transferred to a hydrothermal kettle and at 160 o C, preserving heat for 12 hours; KLuF is carried out 4 Centrifuge washing 3 times with cyclohexane and ethanol mixture (volume ratio 1:3), followed by KLuF 4 The nanocrystals were dispersed in 4 ml cyclohexane; adding 1 ml of dilute hydrochloric acid into the solution, performing ultrasonic treatment in an ultrasonic cleaner with the power of 1 kilowatt for 30 minutes, and centrifugally washing for 1 time by using a mixed solution of ethanol and deionized water (the volume ratio is 1:3), thereby obtaining KLuF 4 The nanocrystals were dispersed in 2 ml deionized water; bismuth subnitrate 0.6 mmol, lutetium acetate 0.4 mmol, glacial acetic acid 10 ml and deionized water 40 ml are stirred at room temperature for 30 minutes to obtainTo solution a; KLuF was added to solution A 4 Stirring the nanocrystalline solution at room temperature for 60 minutes; then, 0.48 mmol of potassium iodide and 0.3 ml of diluted hydrochloric acid were added to the above solution, and stirred at room temperature for 10 minutes; citric acid and EDTA (1:0.8 molar ratio) were then added in a total molar amount of 0.72 mmole), stirred at room temperature for 5 minutes, and then placed at 30 o C, standing for 24 hours in the oven, and finally storing the obtained color developing agent in a black bottle.
Hydrogel preparation
50 mg of prepared citric acid and ethylenediamine tetraacetic acid (EDTA) ligand modified lutetium potassium fluoride-bismuth lutetium iodide compound color reagent is dissolved in (5-20) ml of deionized water, and then 10-15 ml of acrylic acid polyethylene glycol diester is added; ultrasonic treatment for 5-12 hr in ultrasonic apparatus with power greater than 1KW, adding 2-hydroxy-2-methyl-1- [4- (2-hydroxy ethoxy) phenyl ] -1-acetone, and stirring at room temperature for 20-40 min; then irradiating the solution for 15-30 minutes by using a laser with the power of 1-5W and the wavelength of 659 nanometers, and adding 50 microliters of ethylene glycol every 1 minute in the irradiation process to finally obtain the hydrogel with the porous structure.
The color developing agent uniformly distributed in the hydrogel needs to act with a certain amount of uric acid to realize color development, and the color developing agent cannot develop color when the uric acid concentration is lower than the critical value, whereas the color developing agent can develop color when the uric acid concentration is higher than the critical value, and the critical value is defined as a threshold value of the uric acid concentration.
The single urine volume of an adult is generally 200-400 milliliters, the porous structure hydrogel designed by the patent can absorb 5-50 milliliters of urine once, the upper limit of the single uric acid absorption volume is controlled through the size of the hydrogel holes, the larger the holes are, the faster the uric acid absorption speed is, the higher the upper limit of uric acid which can be absorbed is, for example, the single absorption volume is 10 milliliters, and the purpose is to confirm whether the uric acid concentration of a user is higher or not through smaller volume.
The hydrogel prepared by the preparation method comprises 8% of the total mass of the hydrogel, namely the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent coated with the ligand according to the mass percentage, and is suitable for absorbing 10 milliliters of urine, when the amount of urine absorption needs to be regulated, the quality of the color-developing agent in the hydrogel can be regulated, for example, when the quality of the color-developing agent is 65 milligrams, the hydrogel is suitable for absorbing 20 milliliters of urine.
The hydrogel is gel with a three-dimensional network structure formed by induction of a photoinitiator under the irradiation of an external light source, and the color developing agent is uniformly distributed in the three-dimensional network structure of the hydrogel.
The brightness of the color of the developer is changed into a color rendering index through the value of the fluorescence intensity, and the color rendering index card corresponds to the color rendering index of the hydrogel. When the uric acid concentration in urine is in a normal range, namely less than 420 mu mol/L, the color-developing agent does not develop color, the color-developing index is less than 1, and when the uric acid concentration in urine exceeds the normal range, the color-developing agent starts to develop color, and the color-developing index is more than 1. The larger the color rendering index, the larger the magnitude of the uric acid concentration in the urine to be detected from the normal value, and when the color rendering index is larger than 7, the color developer turns orange, and the user needs to pay special attention.
Under different uric acid concentration conditions, the color development intensity of the hydrogel is represented, and the color development intensity value is divided by a special value because the color development intensity value is usually larger, the color development intensity value is converted into a color development index with smaller value, and the color development index card is manufactured by combining the meaning of the uric acid concentration of a human body in physiological medicine, and the actually used hydrogel is compared with the color development index card, so that the uric acid concentration in urine is deduced. The disposable nursing pad with uric acid prompt facility of this patent belongs to the nursing articles for use, because user individual difference, color rendering index and threshold value can not accomplish complete unification, and the precision that its detected is not 100% accurate yet, and this product is particularly useful for slightly uric acid to be used by the user who is high, through detecting the color rendering index every day, promptly through comparing with the color rendering index of own before, judges near-term uric acid concentration situation.
When the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent material coated with the ligand on the surface is used for directly detecting uric acid, the dosage of the color-developing agent is large in order to improve the detection sensitivity, and a small amount of uric acid can cause color development. In order to realize the function of detecting the threshold value, the hydrogel reduces the dosage of the color developing agent in the preparation process, when the mass percentage of the color developing agent is lower than 7%, the color developing sensitivity of the hydrogel is too low to realize the detection of uric acid with low concentration, and when the mass percentage of the color developing agent is higher than 13%, the color developing sensitivity of the hydrogel is too high to realize the threshold value function of uric acid concentration detection.
As shown in figure 1, the results of inductively coupled plasma atomic emission spectrometry show that the ligand-modified bismuth lutetium potassium iodide product contains K, lu, bi, I and F elements in the mole percentages of 17.35%,9.77%,6.12%,34.13% and 32.63%, respectively, which are basically consistent with the mole percentages added in the raw materials, indicating that the obtained product is KLuF 4 @KLu 0.2 Bi 0.8 I 4 . The transmission electron microscopy analysis showed that the product was particles attached to random flakes (fig. 2). The preparation process of the product comprises the following steps: firstly, potassium ions, lutetium ions and fluoride ions in the raw material are combined to form KLuF 4 Then at KLuF 4 Bismuth lutetium potassium iodide is formed on the surface, and then citric acid and EDTA ligand are coated through surface modification.
The citric acid and EDTA ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide product is light white, and after a small amount of uric acid is added into the color developing agent, the color of the product is changed into yellow. The mechanism is explained as follows: the carboxyl in the ligand and the-NH bond in uric acid promote bismuth lutetium potassium iodide and uric acid to form a complex through electrostatic attraction, the complex finally presents yellow, the color of the solution gradually deepens along with the increase of the concentration of uric acid, and the change amplitude of the solution can be characterized through fluorescence spectrum. As shown in FIG. 3, with increasing uric acid concentration, the intensity of yellow light is gradually increased, and a clear positive correlation is exhibited, so that the yellow light can be used as a standard curve for quantitative detection of uric acid.
The ligand coated on the surface of the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent is designed, and the ligand-free modified lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent is prepared for verifying the importance of modifying lutetium potassium fluoride-bismuth lutetium potassium iodide by adopting citric acid and EDTA ligand. As shown in FIG. 4, the addition of uric acid to this developer did not cause a color change, and even when the amount of uric acid was increased to 100. Mu. Mol/liter, the color change did not occur in the developer, indicating that the surface ligand helps promote the binding of the developer to uric acid by surface action to form a complex.
To verify the effect of each ligand separately, citric acid or EDTA single ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide complexes were prepared separately. As shown in FIG. 5, the intensity of yellow light increases very little and there is no color change after uric acid is added to the citric acid ligand-modified lutetium potassium fluoride-bismuth lutetium potassium iodide developer alone. As shown in FIG. 6, the luminescence intensity of the EDTA single ligand-modified lutetium potassium fluoride-bismuth lutetium potassium iodide color developer hardly changes with the change of uric acid concentration. While adding excessive Na to the solution + After the ions, the color reagent turned orange, indicating that EDTA was easily associated with Na + The ions chelate, thereby changing the color of the developer.
FIG. 7 shows the measurement data of uric acid by the hydrogel in the examples, and it can be seen that the fluorescence intensity is not substantially changed when the uric acid concentration is lower than 420. Mu. Mol/L, and the fluorescence intensity is significantly changed when the uric acid concentration exceeds 420. Mu. Mol/L.
The applicant of the patent researches that the lutetium potassium fluoride-bismuth lutetium potassium iodide compound modified by polyacrylic acid, citric acid and EDTA is mainly applied to urine display lines, and uric acid is added into a color developing agent prepared by the lutetium potassium fluoride-bismuth lutetium potassium iodide compound modified by polyacrylic acid, citric acid and EDTA, and because polyacrylic acid and citric acid are synergistic, the detection sensitivity is extremely high, and a very small amount of uric acid can cause color development, so that the function of detecting a threshold value is difficult to realize.
The preparation method adopts a solvothermal method to prepare potassium lutetium fluoride nanocrystalline, uses hydrochloric acid to treat the nanocrystalline to remove surface ligands, then uses the nanocrystalline as a core, adopts a room-temperature stirring method to prepare the potassium lutetium fluoride-bismuth lutetium iodide composite color developing agent, and then prepares the potassium lutetium fluoride-bismuth lutetium iodide composite color developing agent coated by citric acid and EDTA ligands through surface modification. After uric acid is added into the color developing agent, the solution is dark yellow, the brightness of the solution is in direct proportion to the concentration of uric acid, and the method can be well applied to quantitative detection of uric acid by fitting a relationship curve of the intensity of yellow light and the concentration of uric acid. On the basis, urine of normal people is added into the lutetium potassium fluoride-bismuth lutetium potassium iodide color developing agent, yellow color can be obviously seen, the brightness of the yellow color is gradually deepened along with the increase of uric acid concentration, and after the uric acid concentration reaches a certain degree, EDTA can adsorb enough sodium ions, and the color developing agent is changed into yellow or orange color.
The disposable nursing pad set comprises a packaging bag, a color rendering index card and a plurality of disposable nursing articles, wherein the upper surface layer of the disposable nursing articles is provided with the hydrogel with the uric acid detection function.
Claims (9)
1. The hydrogel with the uric acid detection function is characterized by comprising ethylene glycol and a lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent coated with a ligand on the surface, wherein the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color-developing agent coated with the ligand on the surface accounts for 7-13% of the whole hydrogel in percentage by mass;
the ligand coated on the surface of the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent is citric acid and EDTA;
the preparation method of the lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent comprises the following steps:
1) Dissolving lutetium acetate, potassium acetate and ammonium fluoride in deionized water, and stirring;
2) Adding oleic acid and octadecene in the step 1), stirring, transferring to a hydrothermal kettle, and preserving heat;
3) Centrifugally washing the product obtained in the step 2) by using a mixed solution of cyclohexane and ethanol, and then dispersing the obtained nanocrystals in the cyclohexane;
4) Adding dilute hydrochloric acid into the solution obtained in the step 3), performing ultrasonic treatment in an ultrasonic cleaner, and then performing centrifugal washing by using a mixed solution of ethanol and deionized water to disperse the obtained nanocrystalline in the deionized water;
5) Stirring bismuth subnitrate, lutetium acetate, glacial acetic acid and deionized water at room temperature;
6) Adding the nanocrystalline solution obtained in the step 4) into the solution obtained in the step 5), and stirring;
7) Adding potassium iodide and dilute hydrochloric acid into the solution obtained in the step 6), and stirring;
8) And 7) adding citric acid and EDTA into the solution obtained in the step 7), stirring, and then placing in an oven for standing to obtain the color developing agent.
2. The hydrogel with uric acid detection function according to claim 1, wherein the molar ratio of citric acid to EDTA is 1:0.7-0.9.
3. The hydrogel with uric acid detection function according to claim 1, wherein the molecular formula of the lutetium potassium fluoride-bismuth lutetium potassium iodide complex developer is as follows: KLuF4@KLu0.2Bi0.8I4.
4. A hydrogel with uric acid detection as defined in claim 3, wherein the hydrogel absorbs 5-50 ml of urine, the hydrogel does not develop color when the uric acid concentration in the urine is below a threshold value, the hydrogel develops yellow when the uric acid concentration in the urine is above the threshold value, and the uric acid concentration is proportional to the brightness of the color developer.
5. A hydrogel with uric acid detection as defined in claim 4, wherein the threshold value is 420. Mu. Mol/L of uric acid concentration in urine.
6. The hydrogel with uric acid detection function according to claim 1, wherein the hydrogel is prepared from deionized water, polyethylene glycol diacetate, ethylene glycol, 2-hydroxy-2-methyl-1- [4- (2-hydroxyethoxy) phenyl ] -1-propanone and a color developing agent of lutetium potassium fluoride-bismuth lutetium potassium iodide compound coated with a ligand on the surface.
7. The method for preparing a hydrogel with uric acid detection function according to claim 6, wherein (1) 50 mg of the prepared citric acid and ethylenediamine tetraacetic acid ligand modified lutetium potassium fluoride-bismuth lutetium potassium iodide compound color developing agent is dissolved in 5-20 ml of deionized water, and 10-15 ml of acrylic acid polyethylene glycol diester is added; (2) Ultrasonic treatment for 5-12 hr in ultrasonic apparatus with power greater than 1KW, adding 2-hydroxy-2-methyl-1- [4- (2-hydroxy ethoxy) phenyl ] -1-acetone, and stirring at room temperature for 20-40 min; (3) Irradiating the solution with laser with power of 1-5W and wavelength of 659 nm for 15-30 min, and adding 50 microliter of ethylene glycol every 1 min during irradiation to obtain hydrogel with porous structure.
8. A disposable nursing article, characterized in that the upper surface layer is provided with the hydrogel with uric acid detection function as defined in claim 1.
9. A disposable care pad set comprising a package bag, a color rendering index card and a plurality of disposable care products according to claim 8; the brightness of the color displayed by the hydrogel is changed into a color rendering index through the value of the fluorescence intensity, and the color rendering index card corresponds to the color rendering index of the hydrogel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110225080.0A CN112986228B (en) | 2021-03-01 | 2021-03-01 | Hydrogel with uric acid detection function and application of hydrogel to disposable nursing products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110225080.0A CN112986228B (en) | 2021-03-01 | 2021-03-01 | Hydrogel with uric acid detection function and application of hydrogel to disposable nursing products |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112986228A CN112986228A (en) | 2021-06-18 |
| CN112986228B true CN112986228B (en) | 2023-08-29 |
Family
ID=76351563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110225080.0A Active CN112986228B (en) | 2021-03-01 | 2021-03-01 | Hydrogel with uric acid detection function and application of hydrogel to disposable nursing products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112986228B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1450071A (en) * | 2003-05-14 | 2003-10-22 | 华东师范大学 | Synthesis and use of novel thorium color developing reagent metafluoro azo fluorophosphine |
| CN107286928A (en) * | 2017-05-26 | 2017-10-24 | 安徽师范大学 | The detection method and application of up-conversion luminescence nanomaterial of citrate modification and preparation method thereof, hydrogen peroxide or uric acid |
| CN111443082A (en) * | 2020-03-09 | 2020-07-24 | 西安医学院 | Special micro-fluidic paper chip for uric acid detection and detection analysis method |
| CN112369609A (en) * | 2020-10-30 | 2021-02-19 | 云南奥咖生物技术有限公司 | Functional food capable of reducing uric acid and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7445908B2 (en) * | 2003-06-18 | 2008-11-04 | The United States Of America As Represented By The Secretary Of The Army | Detection of oxidizing agents in urine |
-
2021
- 2021-03-01 CN CN202110225080.0A patent/CN112986228B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1450071A (en) * | 2003-05-14 | 2003-10-22 | 华东师范大学 | Synthesis and use of novel thorium color developing reagent metafluoro azo fluorophosphine |
| CN107286928A (en) * | 2017-05-26 | 2017-10-24 | 安徽师范大学 | The detection method and application of up-conversion luminescence nanomaterial of citrate modification and preparation method thereof, hydrogen peroxide or uric acid |
| CN111443082A (en) * | 2020-03-09 | 2020-07-24 | 西安医学院 | Special micro-fluidic paper chip for uric acid detection and detection analysis method |
| CN112369609A (en) * | 2020-10-30 | 2021-02-19 | 云南奥咖生物技术有限公司 | Functional food capable of reducing uric acid and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 二氯苯醌氯亚胺比色测定尿液尿酸;吴辉, 夏寿阳, 董永明, 程国翔;临床检验杂志(第01期);11 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112986228A (en) | 2021-06-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ma et al. | Linker-eliminated nano metal–organic framework fluorescent probe for highly selective and sensitive phosphate ratiometric detection in water and body fluids | |
| Liu et al. | Constructing carbon dots and CdTe quantum dots multi-functional composites for ultrasensitive sensing and rapid degrading ciprofloxacin | |
| Xiong et al. | Sensing in 15 s for aqueous fluoride anion by water-insoluble fluorescent probe incorporating hydrogel | |
| CN101620229B (en) | Hepatits B virus e antibody assay kit and assay method thereof | |
| Ma et al. | A novel ascorbic acid sensor based on the Fe 3+/Fe 2+ modulated photoluminescence of CdTe quantum dots@ SiO 2 nanobeads | |
| Zhu et al. | A novel magnetic fluorescent molecularly imprinted sensor for highly selective and sensitive detection of 4-nitrophenol in food samples through a dual‐recognition mechanism | |
| CN100520369C (en) | Chemiluminescent organophosphorus pesticide residual analyzer and detecting method thereof | |
| Johnson et al. | Adsorption of organic matter at mineral/water interfaces: 3. Implications of surface dissolution for adsorption of oxalate | |
| CN107573933A (en) | A kind of carbon quantum dot copper ion fluorescence probe and its preparation method and application | |
| CN109696430A (en) | A method of measurement Microcystins Concentration | |
| CN101576484B (en) | Reagent and method for detecting concentration of hydrogen peroxide | |
| Preechakasedkit et al. | Gold nanoparticle core–europium (iii) chelate fluorophore-doped silica shell hybrid nanocomposites for the lateral flow immunoassay of human thyroid stimulating hormone with a dual signal readout | |
| CN112986228B (en) | Hydrogel with uric acid detection function and application of hydrogel to disposable nursing products | |
| Alkian et al. | Quantum yield optimization of carbon dots using response surface methodology and its application as control of Fe3+ ion levels in drinking water | |
| CN103865519A (en) | Preparation method for up-conversion luminescent nano-probe and application of up-conversion luminescent nano-probe to measurement of antioxidant activity of substance | |
| CN103482698A (en) | Nanometer blue titanium dioxide colloid and preparation method and application thereof | |
| CN110194951B (en) | Tetraphenyl ethylene derivative fluorescent probe and preparation method thereof | |
| JP4820963B2 (en) | Functional silica particles and uses thereof | |
| CN101864289A (en) | Detecting particle for free tetraiodothyroxine, preparation and application thereof | |
| CN112998965B (en) | Baby diaper with excrement prompt function | |
| CN113017995B (en) | Adult diaper with uric acid prompting function | |
| CN111474167B (en) | Cu-MOF-luminol-H 2 O 2 Detection of Pb by chemiluminescence system 2+ Method (2) | |
| CN112972124A (en) | Disposable nursing pad with uric acid prompt function | |
| CN107655844A (en) | Developer, copper ion colorimetric method sensor and its application method and purposes for copper ion colorimetric method | |
| CN107102052A (en) | Based on the uric acid electrochemical sensor containing active copper carbon point and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |