CN112985953A - Surgical specimen marking method using magnifier and dye - Google Patents
Surgical specimen marking method using magnifier and dye Download PDFInfo
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- CN112985953A CN112985953A CN202110198222.9A CN202110198222A CN112985953A CN 112985953 A CN112985953 A CN 112985953A CN 202110198222 A CN202110198222 A CN 202110198222A CN 112985953 A CN112985953 A CN 112985953A
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- specimen
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000000975 dye Substances 0.000 claims abstract description 38
- 230000003902 lesion Effects 0.000 claims abstract description 14
- 230000036285 pathological change Effects 0.000 claims abstract description 13
- 231100000915 pathological change Toxicity 0.000 claims abstract description 13
- 239000011521 glass Substances 0.000 claims abstract description 10
- 210000004907 gland Anatomy 0.000 claims abstract description 7
- 239000012188 paraffin wax Substances 0.000 claims abstract description 7
- 230000001575 pathological effect Effects 0.000 claims abstract description 7
- 238000010186 staining Methods 0.000 claims abstract description 7
- 210000004204 blood vessel Anatomy 0.000 claims abstract description 5
- MGJURKDLIJVDEO-UHFFFAOYSA-N formaldehyde;hydrate Chemical compound O.O=C MGJURKDLIJVDEO-UHFFFAOYSA-N 0.000 claims abstract description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 21
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 239000003086 colorant Substances 0.000 claims description 4
- 230000005856 abnormality Effects 0.000 claims description 3
- 206010040882 skin lesion Diseases 0.000 claims description 3
- 231100000444 skin lesion Toxicity 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 230000002496 gastric effect Effects 0.000 claims description 2
- 239000003550 marker Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000001356 surgical procedure Methods 0.000 claims 1
- 230000006496 vascular abnormality Effects 0.000 claims 1
- 238000010827 pathological analysis Methods 0.000 abstract description 10
- 238000002360 preparation method Methods 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 14
- 230000009286 beneficial effect Effects 0.000 description 4
- 210000004400 mucous membrane Anatomy 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 2
- 238000012326 endoscopic mucosal resection Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 238000013110 gastrectomy Methods 0.000 description 1
- 238000009802 hysterectomy Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A method for marking a surgical specimen by using a magnifier and dye relates to the field of specimen preparation. The surgical specimen marking method using the magnifier and the dye comprises the following steps: observing the operation specimen under a magnifier, and marking the boundary part of the pathological changes in the operation specimen, the structural special-shaped part in the pathological changes, the blood vessel special-shaped part and the gland expansion part by using dyes; after the dye is dried, the specimen is placed in a formaldehyde water solution for processing for more than 24 hours; making tissues in the staining range of the operation specimen into tissue strips; the tissue strips were embedded in paraffin and made into slides for pathological observation. According to the surgical specimen marking method using the magnifying glass and the dye, the lesion observed by the magnifying glass is marked on the general excision specimen through the dye, so that a pathologist can correspondingly observe under a microscope to improve the pathological diagnosis level and reduce the workload of pathological diagnosis.
Description
Technical Field
The application relates to the field of specimen preparation, in particular to a surgical specimen marking method by using a magnifying glass and dye.
Background
Various skin and mucous membrane specimens are cut by endoscopic mucosal resection, gastrectomy, skin lesion resection, hysterectomy and the like, and all the cut specimens need to be sent to a pathology department for pathological diagnosis. For small specimens, such as specimens resected by endoscopic mucosal resection, pathologists can fix all specimens for observation; for large specimens, a pathologist fixes the site where gross lesions are visible to the naked eye and then observes the site. The fixed specimen can be cut into a strip tissue with the width of about 2mm for observation, and an endoscopist cannot know the gross lesion of the specimen when observing the lesion under a microscope.
The pathological diagnosis method leads to the barrier of communication between clinicians and pathologists, and firstly, the pathologist can not fix the specimen according to the requirements of the clinicians quickly; secondly, doctors cannot correspond pathological changes observed under a microscope to pathological changes observed by clinicians through an amplification endoscope during operation, and information such as structural abnormity, vascular abnormity, pigment change and the like observed by the clinicians through the amplification endoscope cannot be effectively transmitted to the pathological doctors; cellular abnormalities, vascular encroachment, gland abnormalities, etc. observed by pathologists do not directly convey location information to clinicians.
Disclosure of Invention
The application aims to provide a surgical specimen marking method by using a magnifying glass and a dye, which is beneficial for a pathologist to correspondingly observe under a microscope so as to improve the pathological diagnosis level and reduce the workload of pathological diagnosis.
The embodiment of the application is realized as follows:
the embodiment of the application provides a surgical specimen marking method by using a magnifier and a dye, which comprises the following steps:
observing the operation specimen under a magnifier, and marking the boundary part of the pathological changes in the operation specimen, the structural special-shaped part in the pathological changes, the blood vessel special-shaped part and the gland expansion part by using dyes;
after the dye is dried, the specimen is placed in a formaldehyde water solution for processing for more than 24 hours;
making tissues in the staining range of the operation specimen into tissue strips;
the tissue strips were embedded in paraffin and made into slides for pathological observation.
In some alternative embodiments, the dye is selected from at least one of a biological color tone agent, india ink.
In some alternative embodiments, the aqueous solution of formaldehyde has a mass fraction of formaldehyde of 4%.
In some alternative embodiments, the tissue strip is prepared by cutting tissue within a range of 4-5cm outside of the staining marker of the surgical specimen.
In some alternative embodiments, the width of the tissue strip is 2-3 mm.
In some alternative embodiments, the tissue strips are sectioned into slides 3-5 μm thick using paraffin embedded sections.
In some alternative embodiments, when using dyes to mark lesion boundary sites in surgical specimens and structurally-shaped sites, vascular-shaped sites, and gland-expanded sites in lesions, sites of different lesion types are marked with dyes of different colors.
In some alternative embodiments, the magnifying glass is a gastrointestinal scope with magnifying function, a magnifying glass for observing skin lesions, or a solid-state microscope for post-operative observation of specimens.
The beneficial effect of this application is: the method for marking a surgical specimen by using a magnifier and a dye provided by the embodiment comprises the following steps: observing the operation specimen under a magnifier, and marking the boundary part of the pathological changes in the operation specimen, the structural special-shaped part in the pathological changes, the blood vessel special-shaped part and the gland expansion part by using dyes; after the dye is dried, the specimen is placed in a formaldehyde water solution for processing for more than 24 hours; making tissues in the staining range of the operation specimen into tissue strips; the tissue strips were embedded in paraffin and made into slides for pathological observation. The surgical specimen marking method using the magnifier and the dye marks the pathological changes observed by the magnifier on the general excision specimen through the dye, is beneficial to corresponding observation of a pathologist under a microscope to improve the pathological diagnosis level, reduces the workload of pathological diagnosis, meanwhile, the pathologist checks and writes a report according to the dye mark of a clinician, is beneficial to the clinician to trace the pathological change type and improve the clinical diagnosis level, and the method has wide application range, and all excision specimens relating to skin and mucous membrane organs can be marked.
Detailed Description
The features and properties of the surgical specimen marking method using magnifying glasses and dyes of the present application are described in further detail below with reference to examples.
The embodiment provides a surgical specimen marking method by using a magnifier and a dye, which comprises the following steps:
after a clinician cuts out an operation specimen in an operation, the clinician observes the operation specimen under a solid microscope, and uses India ink as fuel to respectively mark a lesion boundary part in the operation specimen and a structural abnormal part, a blood vessel abnormal part and a gland expansion part in the lesion;
after the dye is dried, the marked operation specimen is placed in an aqueous solution of formaldehyde for treatment for more than 24 hours, wherein the mass fraction of the formaldehyde in the aqueous solution of the formaldehyde is 4%;
the tissue within 5cm outside the staining marking range of the operation specimen is made into a tissue strip with the width of 2mm, the tissue strip is embedded by paraffin and then sliced into a slide with the thickness of 5 mu m, a pathologist observes the slide under a microscope, the pathological change corresponds to the endoscopic change according to the dye color of the mucous membrane surface, and finally a pathological report and a pathological recovery report are given.
The surgical specimen marking method using the magnifier and the dye marks lesions on the surgical specimen observed by the magnifier through the dye with different colors, is helpful for corresponding observation of a pathologist under a microscope to improve the pathological diagnosis level, reduces the workload of pathological diagnosis work, writes reports after the pathologist checks the dye marks according to the clinician, is helpful for the clinician to trace back the lesion types, improves the clinical diagnosis level, has wide application range, and can mark all excision specimens related to skin and mucous membrane organs.
In other alternative embodiments, different colors of the bio-color patches may be used for the dyes.
The embodiments described above are some, but not all embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Claims (8)
1. A surgical specimen marking method using a magnifier and a dye is characterized by comprising the following steps:
observing the operation specimen under a magnifier, and marking the boundary part of the pathological changes in the operation specimen, the structural special-shaped part in the pathological changes, the blood vessel special-shaped part and the gland expansion part by using dyes;
after the dye is dried, the specimen is placed in a formaldehyde water solution for processing for more than 24 hours;
making tissues in the staining range of the surgical specimen into tissue strips;
the tissue strips were embedded in paraffin and fabricated into slides for pathological observation.
2. The method of claim 1, wherein the dye is selected from at least one of a bio-color marker and India ink.
3. The method for marking a surgical specimen using a magnifier and a dye according to claim 1, wherein the mass fraction of formaldehyde in the aqueous solution of formaldehyde is 4%.
4. The method of claim 2, wherein the tissue strip is prepared by cutting tissue within a range of 4-5cm outside the surgical specimen staining mark.
5. The method of claim 4, wherein the width of the tissue strip is 2-3 mm.
6. The method of claim 1, wherein the tissue strip is embedded in paraffin and then sliced into a slide with a thickness of 3-5 μm.
7. The method for marking a surgical specimen using a magnifier and a dye according to claim 1, wherein when the dye is used to mark a lesion boundary region in the surgical specimen, a structural abnormality region in a lesion, a vascular abnormality region, and a gland enlargement region, the dye of different colors are used to mark the regions of different lesion types.
8. The method for marking a surgical specimen using a magnifying glass and a dye according to claim 1, wherein the magnifying glass is a gastrointestinal scope having a magnifying function, a magnifying glass for observing skin lesion, or a solid microscope for observing a specimen after surgery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110198222.9A CN112985953A (en) | 2021-02-22 | 2021-02-22 | Surgical specimen marking method using magnifier and dye |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110198222.9A CN112985953A (en) | 2021-02-22 | 2021-02-22 | Surgical specimen marking method using magnifier and dye |
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| Publication Number | Publication Date |
|---|---|
| CN112985953A true CN112985953A (en) | 2021-06-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110198222.9A Pending CN112985953A (en) | 2021-02-22 | 2021-02-22 | Surgical specimen marking method using magnifier and dye |
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Application publication date: 20210618 |
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