CN112985907A - Pre-charging type quantitative suction dilution sample adding device - Google Patents
Pre-charging type quantitative suction dilution sample adding device Download PDFInfo
- Publication number
- CN112985907A CN112985907A CN202110195540.XA CN202110195540A CN112985907A CN 112985907 A CN112985907 A CN 112985907A CN 202110195540 A CN202110195540 A CN 202110195540A CN 112985907 A CN112985907 A CN 112985907A
- Authority
- CN
- China
- Prior art keywords
- sample
- suction
- quantitative
- dilution
- liquid storage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hydrology & Water Resources (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a pre-charging type quantitative suction dilution sample adding device. Specifically, the device provided by the invention comprises (a) a barrel, wherein the barrel sequentially comprises the following components in the direction from a manipulation end to a sample suction end: a limiting part, a quantitative part and an optional liquid storage part; (b) a plunger, the plunger comprising: the push plug comprises a push plug main body, a connecting rod connected with the push plug main body and a knob handle connected with the connecting rod; (c) the tube barrel cover is internally provided with a mixing pool; the tube cover comprises a sample outlet which is communicated with the blending pool in a fluid mode; and (d) a draw needle connected in fluid communication with the sample outlet. In addition, the invention also provides a method for quantitatively absorbing, diluting and adding samples. The device integrates the functions of absorption, dilution, sample addition and the like, is convenient to use and simple to operate, and is not easy to generate errors.
Description
Technical Field
The invention belongs to the field of clinical medical examination; in particular, the invention relates to a prefilling type quantitative suction dilution sample adding device.
Background
With the growing concern for health, the clinical examination in hospitals is increasingly demanded, but because of the limited space of clinical examination departments, the demand for miniaturized and speeded instruments is also increasing. In addition, the detection result is greatly deviated due to the interference phenomenon commonly existing in clinical samples, and the phenomenon is generally avoided or reduced by dilution. However, the miniaturized instrument generally only can retain a simple detection and analysis function, and can peel functions such as absorption, dilution, sample addition and the like from the instrument, so that an operator is required to perform actions such as sample absorption, dilution, uniform mixing, sample addition and the like, and the workload of the operator and the probability of misoperation can be greatly increased.
The typical modes of operation for most products currently on the market are: an operator sucks a clinical sample by means of a pipette gun or a quantitative pipette and the like; then transferring the container to another container, adding the diluent into the container by means of a liquid transfer gun or a quantitative dropper, or pre-filling the diluent when the container is taken out of the warehouse, and omitting the transfer process of the diluent; then diluting and uniformly mixing the clinical samples; and finally, sucking the diluted clinical sample by virtue of a pipette or a quantitative dropper again to perform sample loading. This mode of operation has at least 3 disadvantages: 1) the operation action is complicated: two to three times of sucking action, one time of moving and taking, one time of mixing and one time of sample adding action are needed, so that the use by a user is inconvenient; 2) the required instruments and materials are more: a container for mixing and a pipette gun or a quantitative dropper for pipetting are required, which leads to high cost; 3) two to three aspirating actions require two to three pipette guns or burettes of different sizes, which also increases the risk of operator misuse and misuse.
In view of the above, there is a need in the art for a device that integrates the operations of sucking, diluting, and sample adding, and the device is convenient and simple to use, and is not prone to error.
Disclosure of Invention
The invention aims to provide a prefilled quantitative suction dilution sample adding device. The device is preloaded with required diluent, can integrate the processes of sample suction, dilution, sample adding and the like, and does not need to replace pipetting equipment and pipette.
In a first aspect of the present invention, there is provided a prefilled quantitative suction dilution sampling device, comprising:
(a) a barrel (2), the barrel includes in proper order to inhale the application of sample end direction along controlling the end: a limiting part, a quantitative part and an optional liquid storage part;
(b) a plunger (1) comprising: the push plug comprises a push plug main body (13), a connecting rod (12) connected with the push plug main body (13), and a knob handle (11) connected with the connecting rod;
(c) the device comprises a tube cover (4), wherein a mixing pool is arranged in the tube cover (4); the tube cover (4) is also provided with a sample outlet (41) which is communicated with the blending pool in a fluid manner; and
(d) a draw needle (5) connected in fluid communication with the sample outlet (41);
wherein the content of the first and second substances,
the quantitative part comprises an inner channel (24) with a preset cross section area S (the inner channel can be a tubular inner channel with the inner diameter D), and the plug body (13) is matched with the inner channel (24) so as to enable the plug body (13) to move in the inner channel (24) and enable the plug body (13) and the wall surface of the inner channel (24) to form a sealing structure (61);
the pipe barrel wall of the limiting part is provided with a hollow structure, the hollow structure comprises a strip-shaped sliding groove arranged parallel to the central line of the pipe barrel, and the two ends of the sliding groove in the length direction are respectively a B end and a C end; wherein, the C end is close to the sample suction and adding end; and the sliding groove is configured to enable the knob handle (11) to move along the length direction of the sliding groove, and the longest distance for the knob handle (11) to move in the sliding groove is H2;
a limiting structure (21) is further arranged between the end B and the end C and used for fixing the knob handle (11); the sliding groove is divided into a sucked section (22) and a sample adding section (23) according to a limiting structure; wherein the suction segment (22) is a part from the limiting structure to the end B, the length of the suction segment (22) is H1, the sample adding segment (23) is a part from the limiting structure to the end C, and the length of the sample adding segment is H3;
the liquid storage part comprises a liquid storage space (25) with a preset volume V, wherein the liquid storage space is formed by a tube wall of the liquid storage part, a virtual section between the liquid storage part and the limiting part and a virtual section of a bottom opening (27) of the tube (namely, an opening of the tube close to the sample absorbing end);
the blending pool in the tube cover (4), the liquid storage space (25) of the optional liquid storage part, the inner channel (24) of the quantitative part and the plug main body (13) form a continuous space together.
In another preferred example, the tube is provided with a first connecting unit, and the tube cover (4) is provided with a second connecting unit; and the first and second connection units are mating so that the cartridge and the cartridge cover (4) can be sealingly connected by the first and second connection units.
In another preferred example, the first connecting unit of the tube (1) is inside, and the second connecting unit of the tube cover (4) is outside.
In another preferred example, the first connection unit is an external thread (81) arranged on the outside of the tube wall of the tube and the second connection unit is an internal thread (82) arranged on the inside of the tube cover (4) matching the external thread (81).
In another preferred embodiment, the edge of the bottom opening (27) of the tube is also provided with a sealing ring (26).
In another preferred embodiment, the sealing ring (26) is a rubber ring
In another preferred embodiment, the inner channel (24) is a tubular inner channel.
In another preferred embodiment, the cross-section of the inner channel (24) is circular.
In another preferred embodiment, the inner diameter of the inner channel (24) is D.
In another preferred example, the end of the knob (11) protrudes out of the outer wall of the barrel.
In another preferred example, the limiting structure (21) is a side groove communicated with the sliding groove, and one end of the side groove far away from the sliding groove is an A end.
In another preferred embodiment, the knob handle can be moved completely into the side groove (i.e., the knob handle can be completely out of the sliding groove).
In another preferred example, the distance T between the upper limit side and the lower limit side of the limit structuregThe knob handle has a thickness T + -1% T (preferably + -0.5% T; more preferably + -0.1% T) in the direction of the tube centerline.
In another preferred embodiment, TgT ═ (1 ± 1%); preferably, TgNot (1. + -. 0.5% T; more preferably, Tg＝(1±0.1％)T。
In another preferred embodiment, the width of the side groove (along the tube central line direction), i.e. the distance T between the upper limit side and the lower limit sideg。
In another preferred example, the width W of the sliding groovegAbout 5% W (preferably 2% W; more preferably 1% W; most preferably 0.5% W) of the width of the knob handle in the tangential direction of the knob handle.
In another preferred embodiment, WgW ═ (1 ± 5%) W; preferably, WgW ═ (1 ± 2%); more preferably, WgW ═ (1 ± 1%); optimally, Wg＝(1±0.5％)W。
In another preferred embodiment, the device determines the quantitative suction volume V according to the predetermined section area S of the internal passage (24) and the suction segment length H1Suction device。
In another preferred embodiment, the device determines the range of dilution ratios (i.e., the maximum dilution factor and/or the minimum dilution factor) of the sample based on the predetermined cross-sectional area S of the internal channel (24), the suction segment length H1, and optionally the predetermined volume V of the reservoir space (25).
In another preferred embodiment, the device determines the dosing volume V based on the predetermined cross-sectional area S of the inner channel (24) and the maximum distance H2 that the knob handle (11) can move along the slideAdding。
In another preferred example, H2 is approximately equal to the sum of the suction segment length H1 and the loading segment length H3, i.e., H2 ═ H1+ H3 (1 ± 1%); preferably, H2 ═ (H1+ H3) × (1 ± 0.5%); more preferably, H2 ═ H1+ H3 (1 ± 0.1%).
In another preferred embodiment, the pipetting needle is connected to the sample outlet (41) in a disposable manner.
In another preferred embodiment, the disposable mode means that the sample sucking needle can be separated from the sample outlet (41) of the tube cover (4) by an external force (such as hand or tool).
In another preferred example, the connection mode of the sample outlet (41) and the sample sucking needle comprises a detachable connection (such as a nested connection or a threaded connection) or an integrated connection (such as 3D printing one-step molding).
In another preferred example, when the sample outlet (41) and the sample sucking needle are connected in an integrated mode, the connection part comprises a structure which is easy to break after being stressed.
In another preferred example, the structure which is easy to break after being stressed comprises: and (5) a concave structure.
In another preferred embodiment, the apparatus further comprises: a needle cap (6), and the bottom of the needle cap is provided with a sealing structure (61) for sealing the needle head of the sample sucking needle before use.
In another preferred example, the sealing structure (61) is a rubber pad.
In another preferred example, when the sample suction needle is in a sealed state, the plunger body (13), the inner channel (24), the optional liquid storage pool and the mixing pool are in a sealed state (or form a sealed space together).
In another preferred example, the plunger body (13) and the connecting rod are fixedly connected.
In another preferred embodiment, the link and the knob are fixedly connected.
In another preferred embodiment, the knob moves a certain distance in the tube barrel direction, and the connecting rod can make the plunger body (13) move in the same direction and distance in the tube barrel direction.
In another preferred example, the plug main body (13) is a rubber plug.
In another preferred embodiment, the apparatus further comprises: a barrel cap (3) located at the stopper end of the barrel.
In another preferred embodiment, the tube is a PET tube or a PP tube.
In another preferred example, the mixing pool in the tube cover (4), the liquid storage space (25) of the optional liquid storage part, the inner channel (24) of the quantitative part and the continuous space formed by the push plug main body (13) are filled with diluent.
In another preferred example, the continuous space further includes: the beads were stirred.
In a second aspect of the present invention, there is provided a method for quantitatively pipetting diluted samples, said method comprising the steps of:
(1) providing an apparatus as described in the first aspect;
(2) moving knob (11) of the device to the B end to quantitatively suck the sample;
(3) allowing the sample to be diluted within the device, and
(4) the knob (11) is moved to the C end to carry out quantitative sample adding.
In another preferred example, before moving the knob handle (11) to the end C, the method further comprises the following steps: and removing the sample sucking needle.
It is to be understood that within the scope of the present invention, the above-described features of the present invention and those specifically described below (e.g., in the examples) may be combined with each other to form new or preferred embodiments. Not to be reiterated herein, but to the extent of space.
Drawings
FIG. 1 is a schematic front view of a pre-filled quantitative suction dilution sample feeding device according to an embodiment of the present invention.
FIG. 2 is a schematic side view of a pre-filled quantitative suction dilution loading device according to an embodiment of the present invention.
Fig. 3 shows an internal view of a prefilled quantitative suction dilution sampling device (wherein the cartridge does not include a storage part) in an initial state according to an embodiment of the present invention.
Fig. 4 shows an internal view of a prefilled quantitative suction dilution sampling device (where the cartridge includes a storage part) in an initial state according to another embodiment of the present invention.
Fig. 5a-f are schematic structural views showing components constituting a prefilled quantitative suction dilution sample application device according to the present invention, wherein fig. 5a is a plunger, fig. 5b is a barrel, fig. 5c is a barrel cap, fig. 5d is a barrel cover, fig. 5e is a sample application needle, and fig. 5f is a needle cap.
In each figure, the notation is as follows:
1 is a push plug; 11 is a knob handle; 12 is a connecting rod; 13 is a plug main body;
2 is a pipe barrel; 21 is a limit structure; 22 is a suction section; 23 is a sample adding section; 24 is an inner channel; 25 is a liquid storage space; 26 is a sealing ring; 27 is a bottom opening;
3 is a tube cap;
4 is a tube cover; 41 is a sample outlet;
5 is a sample sucking needle;
6 is a needle cap; 61 is a sealing structure;
71 is a control end; 72 is the sample suction end;
81 is an external thread; and 82 is an internal thread.
Detailed Description
After the present inventors have extensively and intensively studied. The suction dilution sample adding device with the novel structure is designed for the first time, the inside of the device is pre-filled with diluent, and samples can be quantitatively sucked, diluted and then quantitatively added. In addition, the device is simple to operate. The inventors have completed the present invention based on this.
Term(s) for
Expressions such as "upper" and "lower" are used herein for the sake of understanding or convenience of description, but it should be understood that upper and lower refer to a relative positional relationship.
In this context, unless otherwise specified, height or length refers to the distance in the direction of the centerline of the syringe.
Pre-charging type quantitative suction dilution sample adding device
As shown in fig. 1-4 and 5a-f, the present invention provides a pre-filled quantitative suction dilution sample adding device, comprising:
(a) the tube 2 sequentially comprises, along the direction from the manipulation end 71 to the sample suction end 72: a limiting part, a quantitative part and an optional liquid storage part; wherein the tube 2 may be a PET tube or a PP tube;
(b) a plunger 1, said plunger 1 comprising: a plug main body 13 (such as a rubber plug), a connecting rod 12 connected (such as fixedly connected) with the plug main body 13, and a knob handle 11 connected (such as fixedly connected) with the connecting rod; wherein, the knob handle 11 can move a certain distance along a certain direction, and the connecting rod 12 can make the push plug main body 13 move the same distance along a certain direction; preferably, the knob protrudes the end of the knob (11) out of the outer wall of the barrel, making the device easy to handle;
(c) the device comprises a tube cover 4, wherein a mixing pool is arranged in the tube cover 4; the tube cover 4 is also provided with a sample outlet 41 which is communicated with the fluid of the blending pool; and
(d) a pipette tip 5, the pipette tip 5 being connected in fluid communication with the sample outlet 41; preferably, the pipetting needle 5 is connected in a disposable manner to said sample outlet 41; wherein, the disposable mode means that the sample sucking needle 5 can be separated from the sample outlet 41 of the tube cover 4 by an external force (such as hand or tool), so that the sample sucking needle can be omitted to avoid sample residue in the sample sucking needle when the sample is added; the sample outlet 41 and the sample sucking needle 5 may be detachably connected (such as nested connection or threaded connection) or may be integrally connected (such as 3D printing one-step molding), and when the sample outlet is integrally connected, the connection includes a structure (such as a recess or notch structure or other structures with similar functions) which is easily broken after being stressed;
wherein the content of the first and second substances,
the quantitative part comprises an inner channel 24 with a preset sectional area S, and the plug body 13 is matched with the inner channel 24 so that the plug body 13 can move in the inner channel 24 and the plug body 13 and the wall surface of the inner channel 24 form a sealing structure 61;
the pipe barrel wall of the limiting part is provided with a hollow structure, the hollow structure comprises a strip-shaped sliding groove arranged parallel to the central line of the pipe barrel, and the two ends of the sliding groove in the length direction are respectively a B end and a C end; wherein, the C end is close to the sample suction and adding end; and the sliding groove is configured such that the knob handle 11 can move in the long direction of the sliding groove, and the longest distance that the knob handle 11 moves in the sliding groove is H2;
a limiting structure 21 is further arranged between the end B and the end C (the limiting structure 21 can be a side groove communicated with the sliding groove, and one end of the side groove far away from the sliding groove is an end A), and the limiting structure is used for fixing the knob handle 11; the sliding groove is divided into a sucked section 22 and a sample adding section 23 according to a limiting structure; wherein, the suction segment 22 is a part from the limit structure to the end B, the length of the suction segment 22 is H1, the sample adding segment 23 is a part from the limit structure to the end C, and the length of the sample adding segment is H3;
the liquid storage part comprises a liquid storage space 25 with a preset volume V, wherein the liquid storage space is formed by a tube wall of the liquid storage part, a virtual section between the liquid storage part and the limiting part and a virtual section of a bottom opening 27 (namely an opening of the tube close to the sample absorbing end) of the tube;
the above-described construction allows the device of the present invention to determine a quantitative suction volume V based on the predetermined cross-sectional area S of the inner passage 24 and the suction section length H1Suction device(ii) a Determining a range of dilution ratios (i.e., a maximum dilution factor and/or a minimum dilution factor) for the sample based on the predetermined cross-sectional area S of the inner channel 24, the suction segment length H1, and optionally the predetermined volume V of the reservoir space 25; the quantitative sample addition volume V is determined based on the predetermined sectional area S of the inner channel 24 and the maximum distance H2 that the knob handle 11 can move along the slide grooveAdding；
The blending pool in the tube cover 4, the liquid storage space 25 of the optional liquid storage part, the inner channel 24 of the quantitative part and the plug main body 13 together form a continuous space (the continuous space can be preset with diluent and stirring beads for assisting stirring);
optionally a barrel cap 3, and said barrel cap 3 is located at the barrel stopper end (i.e. the side of the manipulation end 71).
An optional needle cap 6, and the bottom of the needle cap is provided with a sealing structure 61 (such as a rubber pad) for sealing the needle head of the sample aspirating needle before use; when the pipette needle is in a sealed state (e.g., closed by the sealing structure 61), the plunger body 13, the inner channel 24, the optional reservoir and the mixing reservoir are in a sealed state (or together form a sealed space).
In another specific embodiment, the tube 1 is provided with a first connection unit (for example, may be an external thread 81 disposed on the outside of the tube wall of the tube), and the tube cover 4 is provided with a second connection unit (for example, may be an internal thread 82 disposed on the inside of the tube cover 4 and matched with the external thread 81) matched with the first connection unit, so that the tube and the tube cover 4 can be hermetically connected through the first connection unit and the second connection unit. Preferably, the rim of the bottom opening 27 of the tube is also provided with a sealing ring 26 (e.g. a rubber ring). Preferably, the first connection unit of the cartridge 1 is inside and the second connection unit of the cartridge cap 4 is outside, so that the device of the invention is easier to pre-load with a larger amount of diluent.
In another preferred example, the absorbing segment refers to a portion from the upper limit side (i.e. the limit side far away from the sample absorbing and adding end) of the limit structure to the B end, and/or the sample adding segment refers to a portion from the lower limit side (i.e. the limit side near the sample absorbing and adding end) of the limit structure to the C end.
In another preferred embodiment, the upper side of the side groove is the upper limiting side, and/or the lower side of the side groove is the lower limiting side.
In another particular embodiment, the apparatus comprises:
the push plug 1 mainly comprises three parts: a knob handle 11, a connecting rod 12 connected with the knob handle, and a push plug main body (such as a rubber plug) 13 connected with the other end of the connecting rod;
a tube barrel 2, wherein the upper half part (i.e. the limiting part) of the tube barrel is hollowed, i.e. a hollowed structure is provided, the hollowed structure comprises a limiting structure 21 which provides an initial position for fixing the plunger and prevents the plunger from shaking when not in use, and a suction section and a sample application section 22 and 23 which are used for enabling the plunger to move therein so as to suck and apply sample, and when the knob of the plunger 1 is moved to the end B and the end C, the knob respectively represents a complete sample suction position and a complete sample application position of the plunger; and the lower barrel half (i.e. the dosing section) is provided with an internal channel 24, said internal channel 24 being intended for the movement of the plunger body 13 of the plunger inside it and optionally for storing part of the diluent.
In another preferred example, the apparatus further includes: a tube cap 3, a sample sucking needle 5 for sucking a sample, and a needle cap 6 for closing the needle head so as to avoid the performance reduction caused by the volatilization of the diluent in the syringe.
In addition, the knob protrudes outside the small device, as shown in fig. 2. As shown in fig. 3, before use, the pipeline is pre-filled with diluent; in the initial state, the knob is fixed at the A position.
It is to be understood that the components of the device are not limited to the shapes, sizes, dimensions, etc. shown in the drawings and/or described herein, and that other structures serving similar functions are within the scope of the present invention.
Application method
The invention also provides a method for quantitatively absorbing, diluting and adding samples, which comprises the following steps:
(1) providing an apparatus as described in the first aspect;
(2) moving knob (11) of the device to the B end to quantitatively suck the sample;
(3) allowing the sample to be diluted within the device, and
(4) the knob (11) is moved to the C end to carry out quantitative sample adding.
In another specific embodiment, step (2) comprises the steps of:
sucking a sample: pushing the knob handle 11 to move the knob handle from the position a to the position B by a height (or distance) H1, and simultaneously, the knob handle 11 drives the push plug 13 to move up by a height H1 through the connecting rod 12; according to the volume formula, if the piston height upward distance is H1 each time, the volume sucked is also constant; wherein the H1 height can be determined according to the amount of sample required to be aspirated; wherein the amount of the aspirated sample can be controlled by adjusting the inner diameter of the inner channel 24, typically, the amount of the aspirated sample (Vaspirate) is between 10-200. mu.L;
in another specific embodiment, step (3) comprises the steps of:
sample mixing and diluting: the absorbed sample and the pre-loaded diluent are mixed in a mixing pool, and the mixture is inverted and mixed for several times to achieve the effect of mixing.
In another specific embodiment, the step (4) comprises the steps of:
sample adding: the knob handle 11 is moved from the position B to the position C, the knob handle moves down by a height H2 (where H2 is H1+ H3), and the plunger body (e.g., rubber plug) 13 is also driven by the connecting rod 12 to move down by a height H2, and the volume of the sample to be added is also constant according to the volume formula. Wherein the height of H2 can be based on the amount of diluted sample (V) added as desiredAdding) To make a determination, typically between 10-200. mu.L.
Through the operation, the device of the invention can simply realize the functions of quantitative suction, dilution and quantitative sample addition.
Preparation method
In another aspect, the present invention also provides a method for preparing the device, comprising the steps of:
(1) providing components of a prefilled quantitative aspiration dilution sample application device, the components comprising: a barrel 2, a plunger 1, a barrel cover 4 and a pipette needle 5, an optional needle cap 6 and an optional barrel cap 3;
(2) assembling the pipe barrel 2 and the push plug 1, and fixing a knob handle 11 of the push plug 1 by a limiting structure;
(3) adding a diluent, optionally adding stirring beads;
(4) the cartridge 2, cartridge cap 4 and the pipetting needle 5, optional needle cap 6 and optional cartridge cap 3 are assembled.
In another specific embodiment, the method of making comprises the steps of:
(1) providing components of a pre-filled quantitative suction dilution sample adding device (as shown in FIGS. 5 a-f);
(2) putting the plunger into the pipe barrel, and adjusting a knob handle 11 on the plunger to a position A of the pipe barrel from an opening position in the pipe barrel;
(3) capping the upper end (i.e. the side of the manipulation end) of the cartridge with a cartridge cap 3 (shown in fig. 5 c) for closing;
inverting the assembled workpiece, and adding diluent from the other opening section (namely the bottom opening 27) of the pipe barrel; 2-3 beads (about 1-2mm in diameter) optionally added during this process, such as beads of glass or polymeric (e.g., polytetrafluoroethylene) material, more preferably polytetrafluoroethylene beads, for subsequent intimate mixing of the sample with the clinical diluent;
(4) screwing a pipe barrel cover 4 and a pipe barrel 1, wherein the pipe barrel 1 is provided with external threads 81, the pipe barrel cover 4 is provided with internal threads 82, and a rubber ring 26 is added at the bottom end of the pipe barrel to play a role in sealing;
optionally linking the pipette tip 5 (shown in FIG. 5 e) to the sample outlet 41 (shown in FIG. 5 d) of the cartridge cap 4 and adding to the workpiece;
the needle cap 6 (as shown in fig. 5 f) is added into the workpiece to protect the sample sucking needle, the bottom end of the needle cap is made of rubber (serving as a sealing structure 61), and the needle head of the sample sucking needle is inserted into the rubber of the needle cap to play a role in sealing, so that performance reduction caused by volatilization of pre-loaded clinical diluent is avoided.
Wherein the sample sucking needle 5 can be integrated with the tube barrel cover 4 or separated from the tube barrel cover, if the sample sucking needle is separated, the sample sucking needle can be abandoned and then the sample is added before the sample is added, and the result misalignment caused by the sample residue in the sample sucking needle can be avoided. If the sample collection needle is integrated, the connection part of the sample collection needle and the sample collection needle can be a structure which is easy to twist off, such as a recess, so that the sample collection needle can be removed before sample collection, and the result misalignment caused by sample residue in the sample collection needle during sample collection can be avoided.
The device of the invention is simple to prepare, and can realize large-scale production and use:
the main advantages of the invention include:
1) the cost is low: the required materials such as PET (polyethylene terephthalate) (the same as that of the vacuum blood collection tube) or PP (polypropylene) (the same as that of the centrifugal tube), rubber and the like are commonly used in the market;
2) the operation is simple and convenient: the step of dilution is reserved, and the product performance and the optimization space are ensured; reagent consumables do not need to be replaced in the whole process, and quantitative sample suction, quantitative sample adding and automatic operation are realized;
3) the project adaptability is strong: the large-span dilution ratio project can be realized, the large-span dilution ratio project is different from 1:1 to 1:10 to 1:500, and the small-ratio dilution project can be adjusted by the small-span dilution device to realize different-ratio dilution processes by adjusting the amount of the pre-filled diluent; large scale dilution program adjustments may be made to achieve high scale dilution by pre-loading larger volumes of clinical diluent by adjusting the diameter and length of the diluent storage compartment at the lower end of the barrel.
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The following examples are experimental procedures without specific conditions noted, or according to the manufacturer's recommendations. Unless otherwise indicated, percentages and parts are percentages and parts by weight.
Example 1 Using the apparatus, item testing requiring 1:10 dilution was performed
Wherein the inner diameter (namely the section diameter of the inner channel 24) of the pipe barrel where the push plug is positioned is 3mm, and the inner diameter of the joint of the pipe barrel and the mixing pool (namely the liquid storage space 25) is 6 mm;
height H1 of A to B is 14.2 mm;
the total amount of clinical diluent pre-filled in the tube barrel is 900 mul, wherein the volume of the clinical diluent in the tube barrel (in the inner channel 24) where the push plug is positioned is 300 mul, the liquid level height is 42.6mm, the volume of the clinical diluent in the tube barrel (namely in the liquid storage space 25) connected with the blending pool is 600 mul, and the liquid level height is 21.3 mm;
wherein two polytetrafluoroethylene microbeads with the diameter of 1.5mm are put in;
the height H2 of B to C was 28.4 mm.
During detection, the knob is used for pushing 11 from A to B, and the volume of the sample sucked upwards can be 100 mu l according to the volume formula V-pi r 2; the clinical diluent and the sample are fully and evenly mixed under the gravity action of the polytetrafluoroethylene in the pipe barrel; then, the knob is pushed 11 from B to C for sample loading, and the volume of the sample punched downwards is also 200 μ l according to the volume formula. Thereby realizing the detection of the items needing 1:10 dilution
Example 2 Using the apparatus, item testing requiring 1:500 dilution was performed
The tube barrel assembly is replaced, wherein the inner diameter of the tube barrel where the push plug is positioned (namely the section diameter of the inner channel 24) is 2mm, the inner diameter of the connection part of the tube barrel and the mixing pool (namely the liquid storage space 25) is 20mm,
height H1 of A to B is 12.7 mm;
the total amount of clinical diluent pre-filled in the pipe barrel is 19960 mu l, wherein the volume of the clinical diluent in the pipe barrel where the push plug is positioned (in the inner channel 24) is 60 mu l, the liquid level height of the clinical diluent is 19.1mm, and the volume of the clinical diluent in the pipe barrel connected with the blending pool (namely in the liquid storage space 25) is 19900 mu l, and the liquid level height of the clinical diluent is 63.4 mm;
wherein three polytetrafluoroethylene microbeads with the diameter of 1.5mm are put in;
the height H2 of B to C was 31.8 mm.
During detection, the knob is used for pushing 11 from A to B, and the volume of the sample sucked upwards can be 40 mu l according to the volume formula V-pi r 2; the clinical diluent and the sample are fully and evenly mixed under the gravity action of the polytetrafluoroethylene in the pipe barrel; then, the knob is pushed 11 from B to C, and the sample volume that is punched out downward is also 100 μ l according to the volume formula. Thereby realizing the detection of the items requiring 1:500 dilution.
All documents referred to herein are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention can be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the present invention as defined by the appended claims.
Claims (10)
1. A pre-filled quantitative aspiration dilution sample application device, comprising:
(a) a barrel (2), the barrel includes in proper order to inhale the application of sample end direction along controlling the end: a limiting part, a quantitative part and an optional liquid storage part;
(b) a plunger (1) comprising: the push plug comprises a push plug main body (13), a connecting rod (12) connected with the push plug main body (13), and a knob handle (11) connected with the connecting rod;
(c) the device comprises a tube cover (4), wherein a mixing pool is arranged in the tube cover (4); the tube cover (4) is also provided with a sample outlet (41) which is communicated with the blending pool in a fluid manner; and
(d) a draw needle (5) connected in fluid communication with the sample outlet (41);
wherein the content of the first and second substances,
the quantitative part comprises an inner channel (24) with a preset sectional area S, and the plug main body (13) is matched with the inner channel (24) so that the plug main body (13) can move in the inner channel (24) and the plug main body (13) and the wall surface of the inner channel (24) form a sealing structure;
the pipe barrel wall of the limiting part is provided with a hollow structure, the hollow structure comprises a strip-shaped sliding groove arranged parallel to the central line of the pipe barrel, and the two ends of the sliding groove in the length direction are respectively a B end and a C end; wherein, the C end is close to the sample suction and adding end; and the sliding groove is configured to enable the knob handle (11) to move along the length direction of the sliding groove, and the longest distance for the knob handle (11) to move in the sliding groove is H2;
a limiting structure (21) is further arranged between the end B and the end C and used for fixing the knob handle (11); the sliding groove is divided into a sucked section (22) and a sample adding section (23) according to a limiting structure; wherein the suction segment (22) is a part from the limiting structure to the end B, the length of the suction segment (22) is H1, the sample adding segment (23) is a part from the limiting structure to the end C, and the length of the sample adding segment is H3;
the liquid storage part comprises a liquid storage space (25) with a preset volume V, wherein the liquid storage space is formed by a tube wall of the liquid storage part, a virtual section between the liquid storage part and the limiting part and a virtual section of a bottom opening (27) of the tube;
the blending pool in the tube cover (4), the liquid storage space (25) of the optional liquid storage part, the inner channel (24) of the quantitative part and the plug main body (13) form a continuous space together.
2. A pre-filled quantitative suction dilution sampling device according to claim 1, wherein the cartridge is provided with a first connection unit, and the cartridge cover (4) is provided with a second connection unit; and the first and second connection units are mating so that the cartridge and the cartridge cover (4) can be sealingly connected by the first and second connection units.
3. A pre-filled metered dose aspirating dilution sample adding device according to claim 2, wherein the first connection unit is an external thread (81) provided on the outside of the cartridge wall of the cartridge, and the second connection unit is an internal thread (82) provided on the inside of the cartridge cover (4) matching with the external thread (81).
4. A pre-filled quantitative suction dilution sampling device according to claim 1,
the device determines a quantitative suction volume V according to a predetermined cross-sectional area S of the inner passage (24) and a suction section length H1Suction device；
The device determines the range of dilution ratios of the sample from a predetermined cross-sectional area S of the inner channel (24), a suction segment length H1 and optionally a predetermined volume V of the reservoir space (25);
the device determines a metered sample volume V based on a predetermined cross-sectional area S of the inner channel (24) and a maximum distance H2 that the knob handle (11) can move along the slide grooveAdding。
5. A pre-filled quantitative suction dilution sampling device according to claim 1, wherein the sample aspirating needle is connected to the sample outlet (41) in a disposable manner.
6. A pre-filled metered dose aspirating dilution sample application device according to claim 1, further comprising: a needle cap (6), and the bottom of the needle cap is provided with a sealing structure (61) for sealing the needle head of the sample sucking needle before use.
7. A pre-filled metered dose aspirating dilution sample application device according to claim 1, further comprising: a barrel cap (3) located at the manipulation end side of the barrel.
8. The pre-filled quantitative suction dilution sample adding device according to claim 1, wherein the mixing well in the cartridge cover (4), the liquid storage space (25) of the optional liquid storage part, the inner channel (24) of the quantitative part, and the continuous space formed by the plunger body (13) are filled with the diluent.
9. A pre-filled metered dose aspirating dilution sample application device according to claim 8, further comprising within said continuous space: the beads were stirred.
10. A method for quantitatively sucking diluted sample application, comprising the steps of:
(1) providing the apparatus of claim 1;
(2) moving knob (11) of the device to the B end to quantitatively suck the sample;
(3) allowing the sample to be diluted within the device, and
(4) the knob (11) is moved to the C end to carry out quantitative sample adding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110195540.XA CN112985907A (en) | 2021-02-19 | 2021-02-19 | Pre-charging type quantitative suction dilution sample adding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110195540.XA CN112985907A (en) | 2021-02-19 | 2021-02-19 | Pre-charging type quantitative suction dilution sample adding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112985907A true CN112985907A (en) | 2021-06-18 |
Family
ID=76394174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110195540.XA Pending CN112985907A (en) | 2021-02-19 | 2021-02-19 | Pre-charging type quantitative suction dilution sample adding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112985907A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114459819A (en) * | 2022-02-09 | 2022-05-10 | 中国人民解放军军事科学院军事医学研究院 | Sample dilution tube and sampling kit using same |
-
2021
- 2021-02-19 CN CN202110195540.XA patent/CN112985907A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114459819A (en) * | 2022-02-09 | 2022-05-10 | 中国人民解放军军事科学院军事医学研究院 | Sample dilution tube and sampling kit using same |
| CN114459819B (en) * | 2022-02-09 | 2024-05-03 | 中国人民解放军军事科学院军事医学研究院 | Sample diluting tube and sampling kit using same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0725593B1 (en) | Fluid sample collection and introduction device | |
| EP1788944B1 (en) | Allergy-testing device | |
| EP3096149A1 (en) | Soft stopper-penetrating pipetting device, and soft stopper-penetrating pipetting method | |
| US20230404544A1 (en) | Self-contained sampling device for processing whole blood | |
| CN112985907A (en) | Pre-charging type quantitative suction dilution sample adding device | |
| US8333711B2 (en) | Blood-drawing device, in particular for infants and small children or small animals | |
| CN214952380U (en) | Pre-charging type quantitative suction dilution sample adding device | |
| US5125278A (en) | Volumetric pipette | |
| US20230138146A1 (en) | Biological Fluid Collection and Stabilization System | |
| EP3318191B1 (en) | System for collecting blood samples from a patient, and corresponding sample collection device and pumps for extracting blood from a patient | |
| US20210114024A1 (en) | Device and Method for Preparing Sample Material | |
| CN218530975U (en) | Push-pull liquid transfer device for sucking viscous liquid | |
| CN214048831U (en) | Liquid sampling device and apparatus, and sampling head or syringe for liquid sampling device | |
| EP2741082A1 (en) | Sampler | |
| CN113349771A (en) | Liquid sampling device and method | |
| CN216485078U (en) | Device for extracting a fluid sample from a closed chamber | |
| KR100624491B1 (en) | Pipette of Pencil Type | |
| CN118122406A (en) | Single-range pipette, pipetting method, separable medicine bin and medicine filling method | |
| CN117615708A (en) | Capillary blood collection device | |
| CN115228518A (en) | Sample quantitative collection and processing assembly | |
| AU2936102A (en) | Adaptor for use with point-of-care testing cartridge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |