CN112980669A - Detachable pore plate for biomacromolecule extraction - Google Patents

Detachable pore plate for biomacromolecule extraction Download PDF

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Publication number
CN112980669A
CN112980669A CN202110289447.5A CN202110289447A CN112980669A CN 112980669 A CN112980669 A CN 112980669A CN 202110289447 A CN202110289447 A CN 202110289447A CN 112980669 A CN112980669 A CN 112980669A
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plate body
base
orifice plate
biomacromolecule
upper portion
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CN202110289447.5A
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Chinese (zh)
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杨庭敢
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
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Abstract

The invention provides a detachable pore plate for biomacromolecule extraction, and relates to the field of pore plates for biomacromolecule extraction. This a can dismantle orifice plate for biomacromolecule draws, the on-line screen storage device comprises a base, base upper end periphery fixedly connected with barrel, the inside evenly distributed in base upper end has the mounting hole, the inside vertical evenly distributed in base upper end has the heating wire, the lower extreme of cooling hole all communicates on cooling tube, the equal fixedly connected with orifice plate body in upper end of installation pole, upper portion all is provided with the draw-in groove in the left side of orifice plate body, upper portion all is provided with the fixture block in the orifice plate body right side, front end and rear end on orifice plate body horizontal direction upper portion all are provided with the screw hole. The clamping grooves and the clamping blocks are connected together to enable the pore plate body to be flexibly connected, the arrangement of the heating wires and the cooling pipeline ensures that the pore plate body is stable in environment when in use, and the pore plate is worth popularizing.

Description

Detachable pore plate for biomacromolecule extraction
Technical Field
The invention relates to the technical field of pore plates for biomacromolecule extraction, in particular to a detachable pore plate for biomacromolecule extraction.
Background
Nucleic acid macromolecules are always combined with various proteins in cells, separation of the nucleic acid macromolecules mainly means separation of nucleic acid from biological macromolecular substances such as proteins and polysaccharide fat, and the following principles should be followed during separation, wherein the principles are firstly to ensure the integrity of primary structures of the nucleic acid macromolecules, and secondly to exclude other molecular pollution, most methods for separating and purifying the nucleic acid macromolecules generally comprise several main steps such as cell lysis, enzyme treatment, separation of the nucleic acid from other biological macromolecular substances, nucleic acid purification and the like, and the method mainly applicable to extraction of the nucleic acid macromolecular substances at present is a magnetic bead method nucleic acid purification technology.
When the nucleic acid macromolecule is extracted by the magnetic bead method nucleic acid purification technology, at first, the related pore plate is required to be used for carrying out the bearing of the biological macromolecule sample, the pore plate existing in the market generally has the following two problems, firstly, the pore plate is mostly connected into a whole at present, the actual biological macromolecule sample amount of nucleic acid is different at present, the pore plate can not be completely used up during the use, the result that leads to like this is that the pore plate uses the inflexibility to lead to waste, secondly, when the nucleic acid macromolecule is extracted at present, most all place the pore plate under the environment of specific construction, the setting of external environment makes the pore plate function single like this.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a detachable pore plate for extracting biomacromolecules, and solves the problems that the pore plate in the existing market is not flexible enough in use and the pore plate cannot create an experimental environment and has single function.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a detachable pore plate for extracting biomacromolecules comprises a base, wherein a barrel body is fixedly connected to the periphery of the upper end of the base, mounting holes are uniformly distributed in the upper end of the base, limiting grooves are transversely and uniformly distributed in the upper end of the base, heating wires are vertically and uniformly distributed in the upper end of the base, the front ends of the heating wires are electrically connected to a power socket, cooling holes are formed in the left side of the rear end of each mounting hole, the lower ends of the cooling holes are communicated with a cooling pipeline, the cooling pipeline is transversely arranged at the bottom in the base, mounting rods are arranged in the mounting holes, circular grooves are arranged on the outer sides of the mounting rods and clamped at the periphery of the limiting rods, a pore plate body is fixedly connected to the upper end of each mounting rod, clamping grooves are formed in the middle upper portion of the left side of the pore plate body, clamping blocks are arranged in the middle upper portion of the right side, the front end and the rear end of the upper part of the orifice plate body in the horizontal direction are both provided with threaded holes.
Preferably, the limiting grooves are communicated with the middle of the front end of the mounting hole, and limiting rods are arranged in the limiting grooves.
Preferably, the power socket is fixedly connected to the upper part of the front end of the base, and the heating wires are all arranged between the mounting holes.
Preferably, the right ends of the cooling pipelines are fixedly connected to the left end of the cooling pipe orifice, and the cooling pipe orifice is arranged at the middle lower part of the right side of the base.
Preferably, the clamping grooves and the clamping blocks of the orifice plate body in the horizontal direction are clamped in sequence.
Preferably, the threaded holes in the horizontal direction in the orifice plate body are all in threaded connection with fixed threaded rods, and two ends of each fixed threaded rod horizontally penetrate through the side walls on two sides of the upper part of the cylinder body.
Preferably, the periphery of the bottom wall of the pore plate body is uniformly distributed with small holes.
Preferably, the equal fixedly connected with stopper in the well upper portion of orifice plate body inner wall, the upper end inboard of stopper all is provided with the sample container.
The working principle is as follows: when the detachable pore plate extracted by biomacromolecules is used, corresponding pore plate bodies are selected according to the number of extracted samples, the pore plate bodies are connected together through the head-tail clamping grooves and the clamping blocks after the pore plate bodies are selected, then the detachable pore plate is placed in the barrel body to enable the installation rod to be inserted into the installation hole, at this time, the limiting rods are inserted into the limiting grooves in the base according to the number of the pore plate bodies in the base, the limiting rods are clamped in the circular grooves at the bottom of the installation rod at this time to enable the pore plate bodies to be fixed in the installation hole, then the fixed threaded rods are inserted into the barrel body and pass through the threaded holes on the pore plate bodies to enable the pore plate bodies to be completely fixed in the barrel body, then the sample containers are placed on the limiting blocks in the pore plate bodies one by one, so that the arrangement of the number of the pore plate bodies is, make the orifice plate use very nimble, avoid the waste of orifice plate, when extracting nucleic acid macromolecule, extract required environmental condition according to nucleic acid macromolecule, can pass through power socket switch on, make the heating wire generate heat, make the base generate heat, can provide certain temperature environment for the orifice plate body, also can let in low temperature nitrogen gas in to the cooling duct through the cooling pipe mouth of pipe, low temperature nitrogen gas passes through in the cooling hole gets into the barrel, inside the orifice plate body is entered into through the aperture again, such setting can guarantee that the sample container can have suitable extraction environment in the experiment, need not add when nucleic acid macromolecule extracts and build the environment, the service function of the orifice plate of increase, guarantee that it can be fine to use, be worth wideling popularize.
(III) advantageous effects
The invention provides a detachable pore plate for extracting biomacromolecules. The method has the following beneficial effects:
1. according to the invention, the pore plate body is selected according to the number of the extracted samples of the nucleic acid macromolecules, the pore plate body is connected with the clamping blocks through the clamping grooves at the head and the tail, then the mounting rod is fixed in the mounting hole through the limiting rod, the fixing threaded rod is inserted into the barrel body and passes through the threaded hole on the pore plate body, so that the pore plate body is completely fixed in the barrel body, the arrangement of the pore plate body is flexibly selected according to the number of the sample selection pore plate bodies, the pore plate has very high utilization rate, the pore plate is very flexible to use, and the waste of.
2. According to the invention, the power socket is connected with a power supply to enable the electric heating wire to generate heat, so that the base generates heat, low-temperature nitrogen can be introduced into the cooling pipeline through the cooling pipe opening, the low-temperature nitrogen enters the cylinder body through the cooling hole and enters the orifice plate body through the small hole, the arrangement can ensure that the sample container can have a proper extraction environment in an experiment, an environment is not additionally created in the extraction of nucleic acid macromolecules, the use function of the orifice plate is increased, the stability of the environment in the extraction of the nucleic acid macromolecules is facilitated, the orifice plate body can be well used, and the method is worthy of great popularization.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a perspective view of the base of the present invention;
FIG. 3 is a cross-sectional view of the base of the present invention;
FIG. 4 is a perspective view of an orifice plate body of the present invention;
fig. 5 is a sectional structural view of an orifice plate body of the present invention.
Wherein, 1, a base; 2. a power outlet; 3. a limiting rod; 4. cooling the pipe orifice; 5. a barrel; 6. fixing a threaded rod; 7. a well plate body; 8. mounting holes; 9. a cooling hole; 10. a limiting groove; 11. a cooling duct; 12. mounting a rod; 13. a card slot; 14. a limiting block; 15. a sample container; 16. a clamping block; 17. a small hole; 18. a circular groove; 19. a threaded hole; 20. an electric heating wire.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
as shown in FIGS. 1-5, the embodiment of the invention provides a detachable orifice plate for biomacromolecule extraction, which comprises a base 1, wherein a cylinder 5 is fixedly connected to the periphery of the upper end of the base 1, mounting holes 8 are uniformly distributed in the upper end of the base 1, limiting grooves 10 are uniformly distributed in the upper end of the base 1 in the transverse direction, heating wires 20 are uniformly distributed in the upper end of the base 1 in the vertical direction, the front ends of the heating wires 20 are electrically connected to a power socket 2, when nucleic acid macromolecules are extracted, a power supply can be switched on through the power socket 2 according to environmental conditions required by nucleic acid macromolecule extraction, the heating wires 20 are heated, the base 1 is heated, a certain temperature environment can be provided for an orifice plate body 7, cooling holes 9 are arranged on the left side of the rear end of the mounting holes 8, the lower ends of the cooling holes 9 are all communicated with a cooling pipeline 11, and the, low-temperature nitrogen is introduced into the cooling pipeline 11 through the cooling pipe orifice 4, the low-temperature nitrogen enters the barrel body 5 through the cooling hole 9 and enters the pore plate body 7 through the small hole 17, the arrangement can ensure that a sample container 15 can have a proper extraction environment in an experiment, an environment is not additionally created when nucleic acid macromolecules are extracted, the use function of the added pore plate is ensured to be well used, when the pore plate body 7 is used, the heating wire 20 and the cooling pipeline 11 are matched for use aiming at different use environments and are flexibly selected, the environment can be created inside the pore plate body 7, the use of extracting different nucleic acids is convenient, the installation rods 12 are arranged inside the installation holes 8, the circular grooves 18 are arranged on the outer sides of the installation rods 12, the circular grooves 18 are all clamped on the periphery of the limiting rod 3, the pore plate body 7 is fixedly connected to the upper ends of the installation rods 12, according to the quantity of orifice plate body 7 in base 1, insert the spacing groove 10 in base 1 with gag lever post 3, gag lever post 3 block is inside the circular slot 18 of installation pole 12 bottom this time, make orifice plate body 7 can fix in mounting hole 8, upper portion all is provided with draw-in groove 13 in the left side of orifice plate body 7, upper portion all is provided with fixture block 16 in the right side of orifice plate body 7, select the orifice plate body 7 of corresponding quantity according to the sample quantity of drawing, link together orifice plate body 7 through draw-in groove 13 and fixture block 16 of head and the tail after selecting orifice plate body 7, then place it inside making installation pole 12 insert the inside of mounting hole 8 at barrel 5, orifice plate body 7 uses very nimble, avoid the waste of orifice plate body 7, front end and the rear end on orifice plate body 7 horizontal direction upper portion all are provided with screw hole 19.
Limiting groove 10 all communicates at the front end middle part of mounting hole 8, all is provided with gag lever post 3 in the limiting groove 10, and inside gag lever post 3 inserted circular slot 18, make installation pole 12 can be fine fixed with mounting hole 8, can not make orifice plate body 7 drop because the removal.
Power socket 2 fixed connection all sets up between mounting hole 8 on the upper portion of base 1 front end, heating wire 20, if orifice plate body 7 need the temperature of height a bit when using, makes power socket 2 switch on, guarantees that base 1 generates heat and makes orifice plate body 7 can be in the temperature environment of suitable operation.
The equal fixed connection of right-hand member of cooling tube 11 is at the left end of cooling pipe orifice 4, and cooling pipe orifice 4 sets up the well lower part on base 1 right side, and cooling tube 11 lets in low temperature nitrogen gas bottom orifice plate body 7 through cooling hole 9, gets into the inside of orifice plate body 7 through aperture 17, has guaranteed sample container 15 in suitable environment.
The hole plate body 7 is sequentially clamped by the clamping grooves 13 and the clamping blocks 16 in the horizontal direction, so that the hole plate bodies 7 in different quantities can be connected, and normal and good use of samples is guaranteed.
The threaded holes 19 in the horizontal direction in the pore plate body 7 are internally and uniformly connected with the fixed threaded rods 6 in a threaded manner, the two ends of each fixed threaded rod 6 horizontally penetrate through the side walls on the two sides of the upper part of the barrel 5, the fixed threaded rods 6 are inserted into the barrel 5 and pass through the threaded holes 19 in the pore plate body 7, and the pore plate body 7 is completely fixed in the barrel 5.
The periphery of the bottom wall of the orifice plate body 7 is uniformly distributed with small holes 17, and the small holes 17 are used for guiding heat in the base 1 into the sample container 15 and also used for guiding nitrogen gas rising from the cooling holes 9 into the orifice plate body 7.
The equal fixedly connected with stopper 14 in the well upper portion of orifice plate body 7 inner wall, the upper end inboard of stopper 14 all is provided with sample container 15, puts sample container 15 into orifice plate body 7 one by one above stopper 14, selects the setting of orifice plate body 7 quantity like this according to the sample, can make orifice plate body 7 have very high utilization ratio.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. A can dismantle orifice plate for biomacromolecule draws, includes base (1), its characterized in that: the barrel body (5) is fixedly connected to the periphery of the upper end of the base (1), mounting holes (8) are uniformly distributed in the upper end of the base (1), limiting grooves (10) are uniformly distributed in the upper end of the base (1) in the transverse direction, heating wires (20) are uniformly distributed in the upper end of the base (1) in the vertical direction, the front ends of the heating wires (20) are electrically connected to the power socket (2), cooling holes (9) are formed in the left side of the rear end of each mounting hole (8), the lower ends of the cooling holes (9) are communicated to the cooling pipeline (11), the cooling pipeline (11) is transversely arranged at the inner bottom of the base (1), mounting rods (12) are arranged in the mounting holes (8), circular grooves (18) are formed in the outer sides of the mounting rods (12), and the circular grooves (18) are clamped to the periphery of the limiting rods (3), the equal fixedly connected with orifice plate body (7) in upper end of installation pole (12), the upper portion all is provided with draw-in groove (13) in the left side of orifice plate body (7), upper portion all is provided with fixture block (16) in orifice plate body (7) right side, front end and the rear end on orifice plate body (7) horizontal direction upper portion all are provided with screw hole (19).
2. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the limiting groove (10) is communicated with the middle part of the front end of the mounting hole (8), and limiting rods (3) are arranged in the limiting groove (10).
3. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the power socket (2) is fixedly connected to the upper portion of the front end of the base (1), and the heating wires (20) are arranged between the mounting holes (8).
4. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the right end of the cooling pipeline (11) is fixedly connected to the left end of the cooling pipe orifice (4), and the cooling pipe orifice (4) is arranged on the middle lower portion of the right side of the base (1).
5. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the hole plate body (7) is clamped with the clamping blocks (16) in sequence in the clamping groove (13) in the horizontal direction.
6. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the hole plate is characterized in that a threaded hole (19) in the horizontal direction in the hole plate body (7) is internally and uniformly connected with a fixed threaded rod (6), and two side walls on the upper portion of the barrel body (5) are horizontally penetrated at two ends of the fixed threaded rod (6).
7. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the periphery of the bottom wall of the pore plate body (7) is uniformly distributed with small holes (17).
8. A removable well plate for biomacromolecule extraction according to claim 1, wherein: the equal fixedly connected with stopper (14) in the well upper portion of orifice plate body (7) inner wall, the upper end inboard of stopper (14) all is provided with sample container (15).
CN202110289447.5A 2021-03-18 2021-03-18 Detachable pore plate for biomacromolecule extraction Pending CN112980669A (en)

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CN202110289447.5A CN112980669A (en) 2021-03-18 2021-03-18 Detachable pore plate for biomacromolecule extraction

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Application Number Priority Date Filing Date Title
CN202110289447.5A CN112980669A (en) 2021-03-18 2021-03-18 Detachable pore plate for biomacromolecule extraction

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CN211999714U (en) * 2019-12-29 2020-11-24 杭州和仪生物科技有限公司 Detection kit for microbial cultivation
CN211988780U (en) * 2020-04-23 2020-11-24 贵州航天医院 Pharmacy test tube heating support
CN112169854A (en) * 2020-09-29 2021-01-05 郑州卓峰制药有限公司 Test tube thermostat for research and development of medical injection
CN112973455A (en) * 2021-03-18 2021-06-18 杨庭敢 Efficient biomolecule splitter

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Publication number Priority date Publication date Assignee Title
CN101186289A (en) * 2006-11-17 2008-05-28 喻维杰 Method for producing lithium iron phosphate material by vacuum rotary kiln
CN103084233A (en) * 2013-01-08 2013-05-08 西安交通大学 Atmosphere-controllable visible cooling device
CN103343877A (en) * 2013-07-26 2013-10-09 武汉钢铁(集团)公司 Method and system for pre-cooling ethylene storage tank by low temperature nitrogen
CN107257710A (en) * 2014-12-10 2017-10-17 弗劳恩霍夫应用研究促进协会 Method and apparatus for the temperature control body of porous plate and for the biological specimen that freezes and/or thaw
CN206253157U (en) * 2016-11-14 2017-06-16 广州品知医疗器械有限公司 A kind of visual constant temp test tube rack device
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CN207042547U (en) * 2017-08-08 2018-02-27 四川省肿瘤医院 A kind of blood specimen frame
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CN109939767A (en) * 2017-12-20 2019-06-28 安徽尤泰克医疗科技有限公司 A kind of Combined test tube bracket
CN208302814U (en) * 2018-03-30 2019-01-01 成都迪盾生物技术有限公司 Metal bath, metal bath lid and metal bath replace external member
CN208297530U (en) * 2018-06-12 2018-12-28 中国人民解放军新疆军区总医院 A kind of detection kit of circulating tumor cell
CN209397189U (en) * 2018-11-06 2019-09-17 武汉瑞博科创生物技术有限公司 A kind of detachable porous plate of nucleic acid extraction
CN109722384A (en) * 2019-03-13 2019-05-07 广州出入境检验检疫局综合技术服务中心 A kind of kit and its application method extracting pathogenic nucleic acid from animal tissue sample
CN210935090U (en) * 2019-08-21 2020-07-07 温州市聚达信息科技有限公司 Test-tube rack for food detection
CN210761951U (en) * 2019-09-30 2020-06-16 乌鲁木齐新大联科生物科技有限公司 Liquid storage device for quantitative detection of protein
CN211216740U (en) * 2019-11-27 2020-08-11 鼓润(武汉)医疗科技有限公司 Detachable EP pipe
CN211999714U (en) * 2019-12-29 2020-11-24 杭州和仪生物科技有限公司 Detection kit for microbial cultivation
CN211253675U (en) * 2019-12-30 2020-08-14 上海市宝山区大场镇祁连社区卫生服务中心 Clinical laboratory examines case with human secretion censorship
CN211913849U (en) * 2020-01-09 2020-11-13 南安市瑞竣机械科技有限公司 Biological multi-functional test-tube rack of using
CN211988780U (en) * 2020-04-23 2020-11-24 贵州航天医院 Pharmacy test tube heating support
CN112169854A (en) * 2020-09-29 2021-01-05 郑州卓峰制药有限公司 Test tube thermostat for research and development of medical injection
CN112973455A (en) * 2021-03-18 2021-06-18 杨庭敢 Efficient biomolecule splitter

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Application publication date: 20210618