CN112971126A - Total nutrient formula food containing GABA for relieving epilepsy and preparation method thereof - Google Patents

Total nutrient formula food containing GABA for relieving epilepsy and preparation method thereof Download PDF

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Publication number
CN112971126A
CN112971126A CN202110304727.9A CN202110304727A CN112971126A CN 112971126 A CN112971126 A CN 112971126A CN 202110304727 A CN202110304727 A CN 202110304727A CN 112971126 A CN112971126 A CN 112971126A
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vitamin
formula food
protein
premix
gaba
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王元秀
李旭
丁良
王彬
张吉方
陈玉晓
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Shandong Huamei Biotechnology Co ltd
University of Jinan
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Shandong Huamei Biotechnology Co ltd
University of Jinan
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    • A23L33/40—Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/175—Amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A23L33/185—Vegetable proteins
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention provides a total nutrient formula food containing GABA for relieving epilepsy and a preparation method thereof, wherein each 100mL of the total nutrient formula food contains: 3-12 g of protein, 5-20 g of grease, 2-8 g of carbohydrate, 5-20 g of rose water extract, 0.1-0.5 g of GABA, 0.01-0.5 g of tryptophan, 0.05-0.5 g of vitamin premix, 1-10 g of mineral premix, 1-10 g of soybean phospholipid and the balance of water; dissolving the nutrients and the soybean phospholipids in water and grease respectively to obtain a water phase and an oil phase, mixing the water phase and the oil phase together, and performing shearing, homogenizing and sterilization to obtain the full-nutrition formula food. The invention utilizes the antioxidation of roses and the action of tryptophan for relieving oxidative stress and the action of GABA for relieving epilepsy by the synergistic action of various components, and prolongs the seizure period of epilepsy.

Description

Total nutrient formula food containing GABA for relieving epilepsy and preparation method thereof
The invention relates to a nutritional preparation, in particular to a full nutritional formula food containing GABA for relieving epilepsy and a preparation method thereof, belonging to the technical field of biology.
Background
Epilepsy is a chronic nervous system disease, at present, the epileptic seizure of most patients can be controlled by medication, but some patients have poor response to the anti-epileptic medication, and the epileptic seizure is difficult to control, so that intractable epilepsy is formed. At present, more than 600 million epileptics in China are present, 65-70 million new epileptics are present each year, and about 25 percent of patients are intractable epileptics. Although the current diagnosis and treatment of epilepsy has advanced greatly, the number of intractable epilepsy patients is increasing.
The onset of epilepsy is caused by an imbalance between central nervous system excitation and inhibition, which is mainly associated with changes in ion channels, neurotransmitters and glial cells. The gene mutation can cause the ion channel dysfunction, namely the defective gene codes defective ion channel protein to cause diseases, wherein the research on the correlation of sodium ion, potassium ion and calcium ion channels and epilepsy is more clear; when excitatory neurotransmitter is too much or inhibitory neurotransmitter is too little, imbalance between excitation and inhibition can be caused, neurons are over-discharged, and epilepsy is caused; seizures can result when the astrocytes have altered uptake of glutamate or gamma aminobutyric acid. Among the inhibitory neurotransmitters important in the central nervous system are gamma-aminobutyric acid (GABA) and glycine, and the excitatory neurotransmitters include glutamic acid (Glu) and aspartic acid.
The first suggestion that epileptogenesis is related to intracerebral GABA was that Tower (Mckhann G M, Tower D B. gamma-amino acids: a substrate for oxidative metabolism of cerebral core [ J ]. The American journel of physiology, 1959, 196(1): 36-38.) shows that The GABA content in brain tissue of experimental convulsive animals-cats is significantly reduced.
In 2009, Fretias et al (Fretias R M D, Nasciento K G D, Ferreira P, et al, Neurochemical change on oxidative stress in a thioppocampus duringphase of pirocarpine-induced epilepsy [ J ]. Pharmacology Biochemistry and Behavior 2010, 94: 341-345.) demonstrated by rat experiments that oxidative stress plays an important role in the onset of epilepsy and that the duration of epilepsy was positively correlated with oxidative damage.
Roses (Rosa rugosa Thunb.) are perennial deciduous shrubs, belong to Rosa (Rosaceae) plants, are mainly produced in France, Russia, Baugalia and other countries, and the main planting areas in China are Shandong, Gansu, Yunnan and other places. In China, roses are also a medicinal and edible plant with a long history. In 2010, the ministry of health in China has approved the Rose with double petals (Rose rugosa cv. plina) as a common food resource for food preparation and processing. According to the record of Chinese pharmacopoeia, the rose bud is warm in nature and sweet in taste, and has the effects of promoting blood circulation, regulating menstruation, tranquilizing and allaying excitement, strengthening spleen, treating traumatic injury and the like.
The extraction method of flos Rosae Rugosae is mainly divided into water extraction method and organic solvent extraction method. Zhanglianli et al (Zhanglianli, Daiweifeng, Shujunha, etc.. analysis of flavonoid components in three rose tea and research on antioxidant activity thereof [ J ]. academic newspaper of Kunming science university (Nature science edition), 2019, 44(05): 84-90.) the content of flavonoid components in three rose tea soups is respectively higher than that in methanol extract thereof; the antioxidant activity of the golden edge and the rose tea soup with flat yin are both higher than that of the methanol extract of the golden edge and the rose tea soup with bitter water, and the antioxidant activity of the rose tea soup with bitter water is lower than that of the methanol extract of the rose tea soup with bitter water. The rose extract obtained by organic solvent extraction method is not suitable for food.
The flos Rosae Rugosae contains essential oil, polyphenols, flavonoids, polysaccharides, etc., and has effects of reducing and eliminating free radicals, resisting oxidation, and resisting bacteria.
Alfreda et al (Alfreda wei, Takayuki Shibamoto. Antioxidant Activities and volabile Consistents of valve Essential Oils [ J ]. J Agric Food Chem, 2007, 55(5): 1737-.
The research on the content of total flavonoids in roses and rose attacking residues [ J ] by ultraviolet spectrophotometry of Setron, Zhang Yina, Limin and the like [ J ] agricultural product processing, 2007(4): 19-20.) and Zhouda and the like [ J ] of rose flavonoids in diabetic mice [ J ] food industry science and technology, 2011, 32(2): 319 and 321 ] finds that the flavonoids in roses have the effects of oxidation resistance and blood sugar reduction.
The study on the extraction and efficacy of the frangipani (frangipani. rose polysaccharide [ D ]. joinan: Shandong light industry academy 2010.) finds that the polysaccharide in the rose has the effects of resisting oxidation and tumors.
Researches on plum jade product 36188.
Tryptophan can relieve oxidative stress (NAYAK B N, BUTTAR H S. Evaluation of the antioxidant properties of tryptophans and its metabolites in the vitamin assay [ J ]. Journal of Complementary and integral Medicine, 2016, 13: 129-136.), the oxidative stress can enhance the catabolism of tryptophan, the amino group of tryptophan can be directly combined with an oxidizing agent, the oxidation reaction of other substances can be effectively prevented, and the antioxidant effect can be achieved. Melatonin, a metabolite of tryptophan, acts as a non-enzymatic antioxidant to scavenge free radicals in the body and also has antioxidant activity.
Tryptophan produces 5-hydroxytryptamine in vivo by the catalytic action of tryptophan hydroxylase and aromatic acid decarboxylase, 5-hydroxytryptamine being the most widely affected in epilepsy (Xu W, Orr-Urrerger A, Nigro F, et al, multiorganic Autonochemical function in the peptide linkage the beta2 and the beta4 cosmetics of neuronal nicotinic acid receptors [ J ]. J Neurosci, 1999, 19(21): 9298. cnotr 9305.), 5-hydroxytryptamine binds to 5-HT3 receptor, causes neuronal depolarization by changing the permeability of Na +, K + and Ca2+, can inhibit epileptic seizures (Zhao H, Lin Y, Chen S, et al. 5-HT3 receptors: a potential therapeutic target for epilepsy [ 2018, Current). The rose and the tryptophan have the effects of eliminating free radicals and resisting oxidation, and can relieve oxidative stress, so that the epilepsy is relieved; meanwhile, tryptophan can be metabolized in vivo to generate 5-hydroxytryptamine, so that epileptic seizure is inhibited, and based on the principle, a full-nutrition formula food containing GABA and used for relieving epilepsia and a preparation method thereof are prepared.
Disclosure of Invention
The invention provides a full-nutrition formula food containing GABA and used for relieving epilepsy and a preparation method thereof, and aims to prolong the seizure period of a patient by utilizing the influence of the metabolism of roses and amino acid in a body on oxidative stress. The invention provides a total nutrient formula food containing GABA for relieving epilepsy, which is an emulsion and is prepared by the following steps:
firstly, the compounding of full-nutrition formula food
The total nutritional formula contains per 100 mL:
3-12 g of protein, wherein the protein is a protein,
5-20 g of grease,
2-8 g of carbohydrate,
5-20 g of rose water extract,
GABA 0.1~0.5 g，
0.01 to 0.5 g of tryptophan,
0.05-0.5 g of vitamin premix,
1-10 g of mineral premix,
1-10 g of soybean lecithin,
the balance being water.
The protein is one or a mixture of more of concentrated whey protein, hydrolyzed whey protein, soy protein isolate and sodium caseinate.
The oil is one or more of soybean oil, peanut oil, linseed oil, perilla seed oil and medium chain triglyceride.
The carbohydrate is one or a mixture of more of maltodextrin and fructo-oligosaccharide.
The rose water extract is filtrate obtained by soaking rose flowers at 70-90 ℃ for 10-20 min according to a material-liquid ratio of 1: 10-1: 25 (g: mL) and filtering, and is concentrated by using a rotary evaporator, wherein the solid content in the filtrate after concentration is 40-60%, polysaccharide is 0.1-0.5 g/L, flavonoid is 0.5-1.5 g/L, and polyphenol is 150-200 g/L.
The mineral premix comprises, by 1g, 30-80 mg of calcium carbonate, 15-20 mg of magnesium chloride, 50-100 mg of edible salt, 80-150 mg of potassium chloride, 20-60 mg of monopotassium phosphate, 0.5-1.0 mg of ferrous sulfate monohydrate, 0.2-0.6 mg of zinc sulfate monohydrate, 0.001-0.01 mg of sodium selenite, 0.03-0.06 mg of anhydrous copper sulfate, 0.01-0.05 mg of manganese citrate, 0.005-0.01 mg of potassium iodide and the balance of a mineral premix carrier.
The mineral premix carrier is maltodextrin.
The vitamin premix comprises, by 1g, 2-5 mg of vitamin A, 0.03-0.06 mg of vitamin D, 250-500 mg of ascorbic acid, 30-60 mg of vitamin E, 10.1-0.5 mg of vitamin K, 3-6 mg of thiamine, 2-6 mg of riboflavin, 8-15 mg of nicotinamide, 15-20 mg of pantothenic acid, 3-6 mg of pyridoxine hydrochloride, 0.5-2.5 mg of folic acid, 0. 120.003-0.020 mg of vitamin B, 0.05-0.25 mg of biotin and the balance of a vitamin premix carrier.
The vitamin premix carrier is maltodextrin.
Preparation of full-nutrient formula food
Water phase: adding protein, carbohydrate, mineral premix, vitamin premix, GABA, tryptophan and rose extract into distilled water at the temperature of 30-60 ℃, and fully stirring until the protein, carbohydrate, mineral premix, vitamin premix, GABA, tryptophan and rose extract are dissolved;
oil phase: adding the emulsifier into the grease, and fully stirring until the emulsifier is dissolved;
uniformly mixing the water phase and the oil phase, and uniformly mixing by using a high-speed shearing machine, wherein the rotating speed is 1500-3000 r/min, and the shearing time is 5-20 min to obtain primary emulsion;
homogenizing the colostrum by using a high-pressure homogenizer under the following homogenizing conditions: homogenizing for 2-8 times at 200-800 bar;
after homogenizing, filling and sterilizing, wherein the sterilization conditions are as follows: 110-125 ℃ for 10-30 min; taking out and cooling to room temperature to obtain the total nutrient formula food.
The full-nutrition formula food provided by the invention contains proteins such as soybean protein isolate and concentrated whey protein, has rich nitrogen source, better supplements the needed protein, is rich in unsaturated fatty acids such as medium-chain fatty acid and DHA, and has important inhibition effect on oxidative stress of epilepsy.
The total nutrient formula food provided by the invention is particularly added with GABA, tryptophan and rose extract, wherein GABA can increase the content of GABA in brain, maintain the balance between excitatory neurotransmitter and inhibitory neurotransmitter in brain, tryptophan can relieve oxidative stress and metabolize to generate 5-hydroxytryptamine, and the rose extract has the effects of reducing and eliminating free radicals, resisting oxidation and the like, relieves oxidative stress and achieves the effect of relieving epilepsy.
The total nutrient formula food provided by the invention adopts carbohydrates with low Glycemic Index (GI) such as maltodextrin, and is beneficial to the control of blood sugar of patients.
The total nutrient formula food provided by the invention is a composite food, combines proteins such as soybean protein isolate, concentrated whey protein and the like, supplements required proteins, combines rose and tryptophan to relieve the oxidative stress of epileptics, combines maltodextrin and other carbohydrates with low GI (glucose) to control the blood sugar of patients, relieves the oxidative stress of epileptics through the synergistic effect of various components, improves the immunity of patients, and relieves the attack of epileptics. The GABA-containing full-nutrition formula food for relieving epilepsy can be used as a nutritional food for providing nutritional support for intractable epilepsy patients, and can also be used as nutritional support for common epilepsy patients.
Example 1:
firstly, the compounding of full-nutrition formula food
The total nutritional formula contains per 100 mL:
6 g of the isolated soy protein is prepared,
10 g of linseed oil, namely 10 g of linseed oil,
4 g of maltodextrin, namely 4 g of maltodextrin,
10 g of rose water extract liquid,
GABA 0.25 g，
0.19 g of tryptophan (tryptophan) is added,
0.2 g of vitamin premix,
5 g of mineral premix, namely 5 g of mineral premix,
4 g of soybean lecithin, namely 4 g of soybean lecithin,
the balance being water.
The rose water extract is filtrate obtained by soaking rose according to the material-liquid ratio of 1:20 (g: mL) at 80 ℃ for 15 min and then filtering, and is concentrated by using a rotary evaporator, wherein the solid content in the filtrate after concentration is 50%, polysaccharide is 0.21 g/L, flavonoid is 0.78 g/L, and polyphenol is 180 g/L.
1g of mineral premix comprises 56 mg of calcium carbonate, 18.3 mg of magnesium chloride, 83 mg of edible salt, 111 mg of potassium chloride, 40 mg of monopotassium phosphate, 0.83 mg of ferrous sulfate monohydrate, 0.4 mg of zinc sulfate monohydrate, 0.003 mg of sodium selenite, 0.044 mg of anhydrous copper sulfate, 0.025 mg of manganese citrate, 0.007 mg of potassium iodide and the balance of maltodextrin.
The vitamin premix 1g comprises 3.6 mg of vitamin A, 0.048 mg of vitamin D, 336 mg of ascorbic acid, 48 mg of vitamin E, 10.264 mg of vitamin K, 4.2 mg of thiamine, 4.2 mg of riboflavin, 12 mg of nicotinamide, 17.4 mg of pantothenic acid, 4.2 mg of pyridoxine hydrochloride, 1.332 mg of folic acid, 120.0078 mg of vitamin B, 0.132 mg of biotin and the balance of maltodextrin. The vitamin premix carrier is maltodextrin.
Preparation of full-nutrient formula food
Water phase: adding soybean protein isolate, maltodextrin, mineral premix, vitamin premix, GABA, tryptophan and rose extract into distilled water of 40 ℃, and fully stirring until the soybean protein isolate, the maltodextrin, the mineral premix, the vitamin premix, the GABA, the tryptophan and the rose extract are dissolved;
oil phase: adding soybean lecithin into linseed oil, and fully stirring until the soybean lecithin is dissolved;
uniformly mixing the water phase and the oil phase, and uniformly mixing by using a high-speed shearing machine, wherein the rotating speed is 2500 r/min, and the shearing time is 15 min to obtain primary emulsion;
homogenizing the colostrum by using a high-pressure homogenizer under the following homogenizing conditions: 600 bar, homogenizing for 4 times;
after homogenizing, filling and sterilizing, wherein the sterilization conditions are as follows: at 115 ℃ for 20 min; taking out and cooling to room temperature to obtain the total nutrient formula food.
Example 2:
firstly, the compounding of full-nutrition formula food
The total nutritional formula contains per 100 mL:
3 g of concentrated whey protein is added into the concentrated whey protein,
5 g of purple perilla seed oil,
2 g of fructo-oligosaccharide,
the rose water extract is 5 g,
GABA 0.1 g，
0.1 g of tryptophan is added in the feed,
0.1 g of vitamin premix,
2 g of mineral premix, namely 2 g of mineral premix,
2 g of soybean lecithin,
the balance being water.
The rose water extract is filtrate obtained by soaking rose according to the material-liquid ratio of 1:10 (g: mL) at 70 ℃ for 20 min and then filtering, and is concentrated by using a rotary evaporator, wherein the solid content in the filtrate after concentration is 60%, polysaccharide is 0.1 g/L, flavonoid is 0.5 g/L, and polyphenol is 150 g/L.
1g of mineral premix comprises 56 mg of calcium carbonate, 18.3 mg of magnesium chloride, 83 mg of edible salt, 111 mg of potassium chloride, 40 mg of monopotassium phosphate, 0.83 mg of ferrous sulfate monohydrate, 0.4 mg of zinc sulfate monohydrate, 0.003 mg of sodium selenite, 0.044 mg of anhydrous copper sulfate, 0.025 mg of manganese citrate, 0.007 mg of potassium iodide and the balance of maltodextrin.
The vitamin premix 1g comprises 3.6 mg of vitamin A, 0.048 mg of vitamin D, 336 mg of ascorbic acid, 48 mg of vitamin E, 10.264 mg of vitamin K, 4.2 mg of thiamine, 4.2 mg of riboflavin, 12 mg of nicotinamide, 17.4 mg of pantothenic acid, 4.2 mg of pyridoxine hydrochloride, 1.332 mg of folic acid, 120.0078 mg of vitamin B, 0.132 mg of biotin and the balance of maltodextrin. The vitamin premix carrier is maltodextrin.
Preparation of full-nutrient formula food
Water phase: adding concentrated whey protein, fructo-oligosaccharide, mineral premix, vitamin premix, GABA, tryptophan and rose extract into distilled water of 30 deg.C, and stirring thoroughly to dissolve;
oil phase: adding soybean phospholipid into perilla seed oil, and stirring to dissolve;
uniformly mixing the water phase and the oil phase, and uniformly mixing by using a high-speed shearing machine, wherein the rotating speed is 1500 r/min, and the shearing time is 20 min to obtain primary emulsion;
homogenizing the colostrum by using a high-pressure homogenizer under the following homogenizing conditions: homogenizing at 200 bar for 8 times;
after homogenizing, filling and sterilizing, wherein the sterilization conditions are as follows: 110 ℃ for 30 min; taking out and cooling to room temperature to obtain the total nutrient formula food.
Example 3:
firstly, the compounding of full-nutrition formula food
The total nutritional formula contains per 100 mL:
12 g of sodium caseinate, namely 12 g,
20 g of medium-chain triglycerides, 20 g,
8 g of maltodextrin, namely 8 g of maltodextrin,
20 g of rose water extract liquid,
GABA 0.5 g，
0.25 g of tryptophan was added thereto,
0.5 g of vitamin premix,
10 g of mineral premix, namely 10 g of mineral premix,
8 g of soybean lecithin (soybean lecithin),
the balance being water.
The rose water extract is filtrate obtained by soaking rose according to the material-liquid ratio of 1:30 (g: mL) at 90 ℃ for 10 min and then filtering, and is concentrated by using a rotary evaporator, wherein the solid content in the filtrate after concentration is 40%, polysaccharide is 0.5 g/L, flavonoid is 1.5 g/L, and polyphenol is 200 g/L.
1g of mineral premix comprises 56 mg of calcium carbonate, 18.3 mg of magnesium chloride, 83 mg of edible salt, 111 mg of potassium chloride, 40 mg of monopotassium phosphate, 0.83 mg of ferrous sulfate monohydrate, 0.4 mg of zinc sulfate monohydrate, 0.003 mg of sodium selenite, 0.044 mg of anhydrous copper sulfate, 0.025 mg of manganese citrate, 0.007 mg of potassium iodide and the balance of maltodextrin.
The vitamin premix 1g comprises 3.6 mg of vitamin A, 0.048 mg of vitamin D, 336 mg of ascorbic acid, 48 mg of vitamin E, 10.264 mg of vitamin K, 4.2 mg of thiamine, 4.2 mg of riboflavin, 12 mg of nicotinamide, 17.4 mg of pantothenic acid, 4.2 mg of pyridoxine hydrochloride, 1.332 mg of folic acid, 120.0078 mg of vitamin B, 0.132 mg of biotin and the balance of maltodextrin. The vitamin premix carrier is maltodextrin.
Preparation of full-nutrient formula food
Water phase: adding sodium caseinate, maltodextrin, mineral premix, vitamin premix, GABA, tryptophan and rose extract into distilled water at 50 ℃, and fully stirring until the sodium caseinate, the maltodextrin, the mineral premix, the vitamin premix, the GABA, the tryptophan and the rose extract are dissolved;
oil phase: adding soybean phospholipid into medium chain triglyceride, and stirring to dissolve;
uniformly mixing the water phase and the oil phase, and uniformly mixing by using a high-speed shearing machine at the rotating speed of 3000 r/min for 10 min to obtain primary emulsion;
homogenizing the colostrum by using a high-pressure homogenizer under the following homogenizing conditions: 800 bar, homogenizing for 2 times;
after homogenizing, filling and sterilizing, wherein the sterilization conditions are as follows: at 121 ℃ for 10 min; taking out and cooling to room temperature to obtain the total nutrient formula food.
The full-nutrition formula food provided by the invention has the following effects on the epileptic attack of mice:
constructing an epilepsia mouse model, taking the test of the example 1-3, only irrigating the stomach, setting a negative control group at the same time, irrigating the stomach with physiological saline of the negative control group, and normally feeding. Kunming mice, male, weight 18-22 g, each group of 10. Mice were gavaged daily for 7 consecutive days at a dose of 0.05 g/10 g. Weighing 1 time every day, observing the disease condition, taking blood from eyeball after last gastric lavage for 1 h, and detecting malondialdehyde content and enzyme activities of superoxide dismutase, glutathione peroxidase and SOD with malondialdehyde detection kit (Nanjing institute of bioengineering), superoxide dismutase detection kit (Nanjing institute of bioengineering), glutathione peroxidase detection kit (Nanjing institute of bioengineering), and SOD commercial detection kit (Biyuntian). The results of the influence of the formula food for special medical application of epileptics on the body weight of a mouse are shown in table 1, the results of the influence on the attack of epilepsy are shown in table 2, and the influences on the malonaldehyde content and the enzyme activity are shown in table 3.
TABLE 1 Effect of the Total nutrient formula on mouse body weight
Initial body weight (g) gain (g)
Example 123.6729.075.40
Example 223.7028.204.50
Example 323.6628.725.06
Negative control group 23.6428.474.83
As can be seen from Table 1, there was no significant difference in the weight gain (P > 0.05) in the mice of examples 1-3, compared to the negative control group. Therefore, the preparation has no obvious influence on the weight increase of mice, and can maintain the normal physiological function of the mice.
TABLE 2 Effect of the Total nutrient formula on the onset of epilepsy in mice
Prolonged attack time compared to negative control group
Example 140 s
Example 230 s
Example 335 s
As can be seen from Table 2, the total nutrient formula food provided by the invention can obviously prolong the seizure cycle of mice and is beneficial to relieving epilepsy.
TABLE 3 Effect of the Total nutrient formula on the blood indices of mice
GSH-Px（μmol/L） CAT（U/g） SOD（U/mL） MDA（nmol/mL）
Example 132.46. + -. 2.6893.71. + -. 10.4819.34. + -. 2.4340.63. + -. 7.25
Example 225.55. + -. 2.2490.08. + -. 20.9118.62. + -. 2.7744.41. + -. 6.53
Example 321.51. + -. 2.6787.42. + -. 21.4718.14. + -. 1.7955.35. + -. 6.40
Negative control group 18.89 + -2.9585.00 + -14.1415.23 + -2.0160.05 + -7.02
As can be seen from Table 3, the total nutrient formula food provided by the invention can improve the enzyme activities of superoxide dismutase, glutathione peroxidase and SOD and reduce the content of malondialdehyde relative to a negative control group, and can relieve the epilepsy of mice.

Claims (8)

1. Firstly, the compounding of full-nutrition formula food
The total nutritional formula contains per 100 mL:
3-12 g of protein, wherein the protein is a protein,
5-20 g of grease,
2-8 g of carbohydrate,
5-20 g of rose water extract,
GABA 0.1~0.5 g，
0.01 to 0.5 g of tryptophan,
0.05-0.5 g of vitamin premix,
1-10 g of mineral premix,
1-10 g of soybean lecithin,
the balance of water;
preparation of full-nutrient formula food
Water phase: adding protein, carbohydrate, mineral premix, vitamin premix, GABA, tryptophan and rose extract into distilled water at the temperature of 30-50 ℃, and fully stirring until the protein, carbohydrate, mineral premix, vitamin premix, GABA, tryptophan and rose extract are dissolved;
oil phase: adding the emulsifier into the grease, and fully stirring until the emulsifier is dissolved;
uniformly mixing the water phase and the oil phase, and uniformly mixing by using a high-speed shearing machine, wherein the rotating speed is 1500-3000 r/min, and the shearing time is 10-20 min to obtain primary emulsion;
homogenizing the colostrum by using a high-pressure homogenizer under the following homogenizing conditions: homogenizing for 2-8 times at 200-800 bar;
after homogenizing, filling and sterilizing, wherein the sterilization conditions are as follows: 110-125 ℃ for 10-30 min; taking out and cooling to room temperature to obtain the total nutrient formula food.
2. The nutritionally complete formula according to claim 1, wherein the protein is one or a mixture of several of concentrated whey protein, hydrolysed whey protein, soy protein isolate and sodium caseinate.
3. The nutritionally complete formula according to claim 1, wherein said oil or fat is one or a mixture of several of soybean oil, peanut oil, linseed oil, perilla seed oil and medium chain triglycerides.
4. The nutritionally complete formula according to claim 1, wherein the carbohydrate is one or a mixture of two of maltodextrin and fructooligosaccharides.
5. The total nutrient formula food as claimed in claim 1, wherein the rose water extract is a filtrate obtained by soaking roses at 70-90 ℃ for 10-20 min according to a material-liquid ratio of 1: 10-1: 25 (g: mL) and filtering, and is concentrated by a rotary evaporator, wherein the solid content in the concentrated filtrate is 40-60%, the polysaccharide content is 0.1-0.5 g/L, the flavonoid content is 0.5-1.5 g/L, and the polyphenol content is 150-200 g/L.
6. The full-nutrition formula food as claimed in claim 1, wherein 1g of the mineral premix comprises 30-80 mg of calcium carbonate, 15-20 mg of magnesium chloride, 50-100 mg of edible salt, 80-150 mg of potassium chloride, 20-60 mg of monopotassium phosphate, 0.5-1.0 mg of ferrous sulfate monohydrate, 0.2-0.6 mg of zinc sulfate monohydrate, 0.001-0.01 mg of sodium selenite, 0.03-0.06 mg of anhydrous copper sulfate, 0.01-0.05 mg of manganese citrate, 0.005-0.01 mg of potassium iodide, and the balance of mineral premix carrier.
7. The total nutrient formula food as claimed in claim 1, wherein 1g of the vitamin premix comprises 2-5 mg of vitamin A, 0.03-0.06 mg of vitamin D, 250-500 mg of ascorbic acid, 30-60 mg of vitamin E, 10.1-0.5 mg of vitamin K, 3-6 mg of thiamine, 2-6 mg of riboflavin, 8-15 mg of nicotinamide, 15-20 mg of pantothenic acid, 3-6 mg of pyridoxine hydrochloride, 0.5-2.5 mg of folic acid, 120.003-0.020 mg of vitamin B, 0.05-0.25 mg of biotin, and the balance of a vitamin premix carrier.
8. The total nutrient formula food according to claim 1, wherein the shearing speed is 1500 to 3000 r/min, and the shearing time is 10 to 20 min; the homogenization conditions were: homogenizing for 2-8 times at 200-800 bar; the sterilization conditions are as follows: 110-125 ℃ for 10-30 min.
CN202110304727.9A 2021-03-23 2021-03-23 Total nutrient formula food containing GABA for relieving epilepsy and preparation method thereof Withdrawn CN112971126A (en)

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Application publication date: 20210618