CN112970587A

CN112970587A - Ginger virus-free asexual breeding method and breeding device

Info

Publication number: CN112970587A
Application number: CN202110463855.8A
Authority: CN
Inventors: 战良; 张吉滔
Original assignee: Qingdao Zexin Agricultural Technology Co ltd
Current assignee: Qingdao Zexin Agricultural Technology Co ltd
Priority date: 2021-04-28
Filing date: 2021-04-28
Publication date: 2021-06-18

Abstract

The invention discloses a ginger virus-free asexual breeding device, which comprises: a cultivation mechanism comprising a base and a cultivation box; the locking mechanism comprises a first threaded rod, two first sliding blocks and two clamping rods; the clamping mechanism comprises a clamping block with a T-shaped longitudinal section, and the clamping block is vertically and fixedly installed on the lower surface of the cultivation box and on the left side wall and the right side wall of the cultivation box and provided with a clamping groove matched with the two clamping rods for use. The two first sliding blocks in the locking mechanism are controlled to drive the two clamping rods to enter and exit from the two clamping grooves formed in the left side wall and the right side wall of the clamping block in the clamping mechanism, so that the cultivation box and the base can be installed and disassembled, the root tip obtained in the ginger virus-free asexual breeding method is part of tissue which is obtained from virus-free plants, then the root tip of the virus-free ginger obtained by adopting a tissue culture technology is used, and the ginger plants cultured by the root tip are used as stock seeds, so that more virus-free gingers can be bred.

Description

Ginger virus-free asexual breeding method and breeding device

Technical Field

The invention relates to the technical field of artificial planting, in particular to a ginger virus-free asexual breeding device.

Background

The ginger refers to a rhizome of a ginger plant, is warm in nature, and the unique gingerol can stimulate gastrointestinal mucosa, so that the gastrointestinal tract is congested and the digestion capability is enhanced, the abdominal distension, the abdominal pain, the diarrhea, the vomiting and the like caused by too much cold and cool food can be effectively treated, the ginger is more and more needed in life, so that how to better cultivate the ginger becomes the important factor in the field of artificial planting, the conventional ginger is generally used as a breeding device in the breeding process, and the pre-rooting obtained by processing the root tip of the ginger plant through some processing steps is mainly placed into a cultivation box for cultivation.

The cultivation box and the base are required to be disassembled to be cleaned after the cultivation work is finished, however, the installation mode between the conventional cultivation box and the base is usually realized through fixing parts such as screws and nuts, the disassembling and installing steps are complicated although stable, so that the working efficiency of workers for cleaning the cultivation box is greatly influenced, the quality of the conventional ginger is often declined due to the infection of plant viruses in the asexual breeding process, the production yield is further reduced, and therefore, how to cultivate the virus-free ginger also becomes an important research subject.

Disclosure of Invention

The invention aims to solve the following defects in the prior art that the cultivation box and the base are required to be disassembled for cleaning after cultivation work is finished, but the conventional cultivation box and the base are usually installed through fixing parts such as screws and nuts, and the disassembling and installing steps are complicated although the conventional cultivation box and the base are stable, so that the working efficiency of workers for cleaning the cultivation box is greatly influenced.

In order to achieve the purpose, the invention adopts the following technical scheme:

a ginger virus-free asexual breeding device comprises:

the cultivation mechanism comprises a base and a cultivation box, a cultivation groove for planting the ginger is formed in the upper surface of the cultivation box, and a box cover is rotatably installed on the upper surface of the cultivation box through a rotating shaft;

the locking mechanism comprises a first threaded rod, two first sliding blocks and two L-shaped clamping connecting rods, wherein the upper surface of the base is provided with a mounting groove, the first threaded rod is rotatably mounted in the mounting groove, the directions of threads on the left side and the right side of the first threaded rod are opposite, the two first sliding blocks are symmetrically sleeved on the left side and the right side of the first threaded rod in a threaded manner, and one ends of the two first sliding blocks, which are far away from the bottom of the mounting groove, are respectively and fixedly connected with one ends of the two clamping;

the clamping mechanism comprises a clamping block with a T-shaped longitudinal section, the clamping block is vertically and fixedly arranged on the lower surface of the cultivation box, and clamping grooves matched with the two clamping rods are formed in the left side wall and the right side wall of the cultivation box;

a drive assembly for providing a drive force for rotating the first threaded rod;

and the limiting component is used for limiting the two first sliding blocks to rotate together when the two first sliding blocks cannot rotate along with the first threaded rod.

Preferably, drive assembly includes worm, worm wheel and first regulating wheel, the base is worn out and is connected with first regulating wheel fixed mounting in the mounting groove and bottom to the perpendicular fixed mounting of worm, the fixed cover of worm wheel is connected on first threaded rod and with the worm meshing.

Preferably, spacing subassembly includes two pulleys, two the pulley is close to the one end fixed connection of mounting groove tank bottom with two first sliders respectively, the spacing groove has been seted up to mounting groove tank bottom level, two the pulley all with spacing groove cell wall rolling contact.

Preferably, a rotating cavity is formed in the base, a bearing is fixedly sleeved on the worm, and an outer ring of the bearing is fixedly connected with the wall of the rotating cavity.

Preferably, the lower surface of the cultivating box is provided with a plurality of water outlet holes communicated with the cultivating groove.

Preferably, cultivate the equal fixed mounting of lateral wall about the box and have the installation shell, the second threaded rod is installed to the installation shell internal rotation, the second slider has been cup jointed to the screw thread on the second threaded rod, cultivate about the box the lateral wall all set up perpendicularly with cultivate the mouth that slides that the groove is linked together, two the equal fixed mounting in one side that the second slider is close to each other has the subassembly of cutting earth, two the subassembly of cutting earth passes two slides respectively and gets into and cultivate the inslot, installation shell and fixed mounting have the second regulating wheel are worn out on the top of second threaded rod.

Preferably, the soil compacting assembly comprises a mounting plate, a spring rod and a pressing plate, the mounting plate is fixedly connected with the side wall of the second sliding block, the lower surface of the mounting plate is fixedly connected with the spring rod in a perpendicular mode, and the pressing plate is fixedly connected with one end, far away from the mounting plate, of the spring rod.

Preferably, the mounting panel lower surface has been seted up the spout, slidable mounting has the slide in the spout, two the equal fixed mounting in one side that the slide is close to each other has the pull rod.

Preferably, a first magnetic block is fixedly mounted at the bottom of the clamping groove, a second magnetic block is fixedly mounted at one end, close to each other, of each of the two clamping rods, and the magnetic poles of the surfaces, close to each other, of the first magnetic block and the second magnetic block are opposite.

In the invention, the virus-free asexual propagation method for the ginger comprises the following steps:

s1: when the ginger is mature or germinates, obtaining part of tissues planted without viruses from plants infected with the viruses, and then obtaining disease-free plants, namely virus-free ginger, by adopting a tissue culture technology;

s2: taking 1-2 cm of the root tip of the detoxified ginger plant obtained in the step S1, cleaning the root tip with clear water, rinsing the root tip with 70% alcohol for 30S, then sterilizing the root tip with 0.1% HgCl2 solution for 3 minutes, and washing the root tip with sterile water 3 over the root tip;

s3: placing the root tip obtained in the step S2 under a microscope, soaking the root tip in alcohol, then burning and cooling forceps and scissors to cut the cells in the meristematic region of the root tip into 3-7 root tip segments, and cleaning 3 parts of the root tip segments with sterile water;

s4: putting the root tip segments obtained in the step S3 into an induction culture medium for culturing for 15-25 days, wherein the induction culture medium is MS + 6-benzylpurine 1.5mg/L + naphthylacetic acid 1.0mg/L to obtain pre-rooted roots, cutting 1-2 cm of the root tips of the pre-rooted roots into 3-7 root tip segments, inoculating the segments into the induction culture medium for culturing for 15-25 days, repeating the steps until a proper number of pre-rooted roots are obtained, inoculating 25-30 pre-rooted roots into a culture box (2) in a culture device in batches, and culturing until cluster buds of 3-4 cm grow out;

s5: putting the long-leaf long-root seedlings obtained in the step S4 in the sun for 3-5 days, taking out the seedlings, transplanting the seedlings to a seedling bed, wherein the row spacing is 5-6 cm, the plant spacing is 5-6 cm, the opening depth is 3-4 cm, one seedling is planted at each position, the seedling bed is kept moist, the seedling bed is prepared by taking sugarcane leaves as a matrix and mixing filter mud of a cane sugar factory and/or sludge of a fish pond, dipotassium phosphate, peanut bran, humus, manganese sulfate and zinc sulfate for fermentation;

s6: transplanting the seedlings to a field when 6-7 compound leaves grow out, wherein the row spacing is 40-60 cm, and the plant spacing is 40-60 cm; the field is selected from sandy loam which has deep soil layer, is fertile, is convenient to drain and irrigate and faces the sun;

s7: fertilizing 5-7 kg per mu at regular intervals, and harvesting when the fertilizer is mature.

Compared with the prior art, the invention has the beneficial effects that:

1. the installation and the dismantlement between box and the base just can be realized cultivating fast to two joint grooves that the lateral wall was seted up about the lateral wall about the staff passes through two first slider area two joint pole business turn over clamping mechanism in the drive assembly control locking mechanical system, and convenient and fast has avoided using the loaded down with trivial details of mounting such as screw nut, has improved the work efficiency that the staff cleared up and cultivates the box.

2. When putting into the cultivation box with the root tip of ginger plant through some processing steps and the pre-rooting that obtains and cultivate the work, only need can control the second threaded rod through the second regulating wheel and take place to rotate, thereby the second slider of screw sleeve joint on the second threaded rod just can take the firming subassembly to carry out the compaction work to the earth of cultivating the inslot, do not need artificial to stretch into the cultivation inslot with the hand and carry out the firming work, and in order to avoid destroying the pre-rooting at the in-process of firming, can also adjust the area of contact between firming subassembly and the earth.

3. The root tip of the plant obtained in the asexual breeding method of the ginger is the root tip of the virus-free ginger obtained by tissue culture technology, and the ginger plant cultured by the root tip is used as an original seed, so that more virus-free gingers can be bred, the quality degradation of crops caused by the infection of plant viruses is avoided, and the yield is improved.

Drawings

FIG. 1 is a schematic front structural diagram of a ginger virus-free asexual propagation device provided by the present invention;

FIG. 2 is a schematic top view of the apparatus for virus-free asexual propagation of ginger according to the present invention;

FIG. 3 is a schematic view of a portion of the enlarged structure of A in FIG. 1;

FIG. 4 is a schematic view of a portion of B in FIG. 1;

fig. 5 is a partially enlarged structural diagram of C in fig. 1.

In the figure: the device comprises a base 1, a cultivation box 2, a cultivation groove 3, a box cover 4, a clamping block 5, a clamping groove 6, a first threaded rod 7, a first sliding block 8, a clamping rod 9, a mounting groove 10, a worm 11, a worm wheel 12, a first adjusting wheel 13, a pulley 14, a limiting groove 15, a rotating cavity 16, a bearing 17, a water outlet hole 18, an installation shell 19, a second threaded rod 20, a second sliding block 21, a sliding opening 22, a second adjusting wheel 23, an installation plate 24, a spring rod 25, a pressing plate 26, a sliding groove 27, a sliding plate 28, a pull rod 29, a first magnetic block 30 and a second magnetic block 31.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1 to 5, a ginger virus-free asexual propagation apparatus includes:

the cultivation mechanism comprises a base 1 and a cultivation box 2, a cultivation groove 3 for planting the ginger is formed in the upper surface of the cultivation box 2, and a box cover 4 is rotatably installed on the upper surface of the cultivation box 2 through a rotating shaft;

the locking mechanism comprises a first threaded rod 7, two first sliding blocks 8 and two L-shaped clamping rods 9, a mounting groove 10 is formed in the upper surface of the base 1, the first threaded rod 7 is rotatably mounted in the mounting groove 10, the directions of threads on the left side and the right side of the first threaded rod are opposite, the two first sliding blocks 8 are symmetrically sleeved on the left side and the right side of the first threaded rod 7 in a threaded manner, and one ends, far away from the bottom of the mounting groove 10, of the first sliding blocks are respectively and fixedly connected with one ends of the two clamping rods;

the clamping mechanism comprises a clamping block 5 with a T-shaped longitudinal section, the clamping block 5 is vertically and fixedly installed on the lower surface of the cultivation box 2, and clamping grooves 6 matched with the two clamping connection rods 9 are formed in the left side wall and the right side wall of the cultivation box;

the driving component is used for providing driving force for rotating the first threaded rod 7;

and the limiting component is used for limiting the two first sliding blocks 8 from rotating together with the first threaded rod 7.

In the embodiment applying the above technical scheme, firstly, a worker vertically puts the cultivation box 2 on the upper surface of the base 1, the clamping mechanism is positioned in the mounting groove 10, at the moment, the first threaded rod 7 is controlled to rotate by the driving component, at the moment, the two first sliding blocks 8 which are in threaded sleeve connection with the first threaded rod 7 can move in the horizontal direction under the action of the limiting component, because the left and right threads of the first threaded rod 7 are opposite in direction, when the first threaded rod 7 rotates, the two first sliding blocks 8 can be driven to approach or separate from the clamping rod 9 which is fixedly installed with the clamping rod 8, the two clamping grooves 6 which are formed on the left and right side walls of the clamping block 5 in the clamping mechanism are controlled to allow the two clamping rods 9 to enter and exit, so that the cultivation box 2 and the base 1 can be quickly installed and detached, the operation is convenient and fast, and the complexity of using fixing, the working efficiency of the worker cleaning the cultivation box 2 and the base 1 is improved.

In the preferred technical scheme of this embodiment, the driving assembly includes a worm 11, a worm wheel 12 and a first regulating wheel 13, the worm 11 is vertically and fixedly installed in the installation groove 10, the bottom end of the worm 11 penetrates through the base 1 and is fixedly installed with the first regulating wheel 13, and the worm wheel 12 is fixedly sleeved on the first threaded rod 7 and is meshed with the worm 11. The first adjusting wheel 13 is rotated, and the worm 11 is rotated, so that the worm wheel 12, which is engaged with the worm wheel and fixedly secured to the first threaded rod 7, rotates with the first threaded rod 7.

According to the preferable technical scheme in the embodiment, the limiting component comprises two pulleys 14, the two pulleys 14 are fixedly connected with one ends, close to the bottoms of the mounting grooves 10, of the two first sliding blocks 8 respectively, the limiting grooves 15 are horizontally formed in the bottoms of the mounting grooves 10, and the two pulleys 14 are in rolling contact with the wall of each limiting groove 15. The two pulleys 14 can only move in the horizontal direction in the limiting groove 15, so that the two first sliding blocks 8 fixedly connected with the two pulleys 14 respectively can only move in the horizontal direction when the first threaded rod 7 rotates, and cannot rotate along with the first threaded rod.

In the preferred technical scheme in this embodiment, a rotating cavity 16 is formed in the base 1, a bearing 17 is fixedly sleeved on the worm 11, and an outer ring of the bearing 17 is fixedly connected with the cavity wall of the rotating cavity 16. The bearing 17 limits the rotation range of the worm 11 and ensures that the worm does not shift in position during rotation.

In the preferred technical scheme of the embodiment, the lower surface of the cultivating box 2 is provided with a plurality of water outlet holes 18 communicated with the cultivating groove 3. After watering, the excess water can flow out of the water outlet hole 18 and fall into the mounting groove 10 under the action of the gravity of the user to be stored and then recycled.

The preferred technical scheme in this embodiment, cultivate the equal fixed mounting of lateral wall and have installation shell 19 about box 2, second threaded rod 20 is installed to the installation shell 19 internal rotation, second threaded rod 20 goes up the screw thread and has cup jointed second slider 21, cultivate the box 2 about the lateral wall all set up perpendicularly with cultivate the mouth 22 that is linked together of groove 3, the equal fixed mounting in one side that two second sliders 21 are close to each other has the soil compacting subassembly, two soil compacting subassemblies pass two smooth mouthful 22 respectively and get into in cultivating groove 3, installation shell 19 and fixed mounting have second regulating wheel 23 are worn out on the top of second threaded rod 20. Only need can control second threaded rod 20 through second regulating wheel 23 and take place to rotate to the second slider 21 of screw sleeve joint on second threaded rod 20 just can take the earth of firming subassembly to carry out the compaction work in the culture trough 3, does not need artificial to stretch into the culture trough 3 with the hand and carries out the firming work.

In the preferred technical scheme of this embodiment, the soil compacting assembly includes a mounting plate 24, a spring rod 25 and a pressing plate 26, the mounting plate 24 is fixedly connected with the side wall of the second slider 21, the lower surface of the mounting plate is vertically and fixedly connected with the spring rod 25, and the pressing plate 26 is fixedly connected with one end of the spring rod 25 far away from the mounting plate 24. The in-process that the soil pressing assembly moved down, clamp plate 26 can at first contact with earth, continues to push down this moment, and the spring beam 25 can take place the shrink to play the effect of buffering, clamp plate 26 just will extrude earth this moment, guarantee the tight real degree of earth.

In the preferred technical scheme of this embodiment, the lower surface of the mounting plate 24 is provided with a sliding groove 27, a sliding plate 28 is slidably mounted in the sliding groove 27, and a pull rod 29 is fixedly mounted on the surface of each of the two sliding plates 28 which is close to each other. In order to avoid damaging the pre-rooting during the soil compacting process, the contact area between the soil compacting assembly and the soil can be adjusted, and the sliding plate 28 can be controlled to move in the sliding groove 27 by pulling the pull rod 29, so that the contact area between the soil compacting assembly and the soil is changed.

In the preferred technical scheme in this embodiment, a first magnetic block 30 is fixedly mounted at the bottom of the groove in the clamping groove 6, a second magnetic block 31 is fixedly mounted at one end of each of the two clamping rods 9 close to each other, and the magnetic poles of the surfaces of the first magnetic block 30 and the second magnetic block 31 close to each other are opposite. When two clamping rods 9 enter the two clamping grooves 6, the first magnetic block 30 and the second magnetic block 31 are gradually close to and tightly attached to each other, and a suction force is generated at the moment, so that the clamping of the clamping rods 9 in the clamping grooves 6 is more stable.

The virus-free asexual propagation method of the ginger comprises the following steps:

s4: putting the root tip segments obtained in the step S3 into an induction culture medium for culturing for 15-25 days, wherein the induction culture medium is MS + 6-benzyl purine 1.5mg/L + naphthylacetic acid 1.0mg/L to obtain pre-rooted roots, cutting 1-2 cm of the root tips of the pre-rooted roots into 3-7 root tip segments, inoculating the segments into the induction culture medium for culturing for 15-25 days, repeating the steps until a proper number of pre-rooted roots are obtained, inoculating 25-30 pre-rooted roots into a culture box 2 in a culture device in batches, and culturing until 3-4 cm of cluster buds grow out;

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A ginger detoxification asexual propagation device is characterized by comprising:

the cultivation mechanism comprises a base (1) and a cultivation box (2), a cultivation groove (3) for planting the ginger is formed in the upper surface of the cultivation box (2), and a box cover (4) is rotatably installed on the upper surface of the cultivation box (2) through a rotating shaft;

the locking mechanism comprises a first threaded rod (7), two first sliding blocks (8) and two L-shaped clamping rods (9), wherein an installation groove (10) is formed in the upper surface of the base (1), the first threaded rod (7) is rotatably installed in the installation groove (10), the directions of threads on the left side and the right side are opposite, the two first sliding blocks (8) are symmetrically sleeved on the left side and the right side of the first threaded rod (7) in a threaded manner, and one end, far away from the bottom of the installation groove (10), of each first sliding block is respectively and fixedly connected with one end of each clamping rod (9) in a perpendicular mode;

the clamping mechanism comprises a clamping block (5) with a T-shaped longitudinal section, the clamping block (5) is vertically and fixedly installed on the lower surface of the cultivation box (2), and clamping grooves (6) matched with the two clamping connection rods (9) are formed in the left side wall and the right side wall of the cultivation box;

a drive assembly for providing a driving force for rotating the first threaded rod (7);

and the limiting component is used for limiting the two first sliding blocks (8) to rotate together when the first threaded rods (7) rotate.

2. The ginger virus-free asexual propagation device according to claim 1, wherein the driving assembly comprises a worm (11), a worm wheel (12) and a first adjusting wheel (13), the worm (11) is vertically and fixedly installed in the installation groove (10) and the bottom end of the worm passes through the base (1) and is fixedly installed with the first adjusting wheel (13), and the worm wheel (12) is fixedly sleeved on the first threaded rod (7) and is engaged with the worm (11).

3. The ginger detoxification asexual reproduction device according to claim 1, wherein the limiting component comprises two pulleys (14), the two pulleys (14) are respectively and fixedly connected with one ends of the two first sliding blocks (8) close to the bottom of the installation groove (10), a limiting groove (15) is horizontally formed at the bottom of the installation groove (10), and both pulleys (14) are in rolling contact with the wall of the limiting groove (15).

4. The ginger virus-free asexual propagation device according to claim 2, characterized in that a rotating cavity (16) is formed in the base (1), a bearing (17) is fixedly sleeved on the worm (11), and an outer ring of the bearing (17) is fixedly connected with the cavity wall of the rotating cavity (16).

5. The ginger virus-free asexual propagation device according to claim 1, wherein the lower surface of the cultivating box (2) is provided with a plurality of water outlet holes (18) communicated with the cultivating groove (3).

6. The ginger virus-free asexual breeding device according to claim 1, characterized in that the left and right side walls of the cultivating box (2) are both fixedly provided with a mounting shell (19), a second threaded rod (20) is rotatably mounted in the mounting shell (19), a second sliding block (21) is sleeved on the second threaded rod (20) in a threaded manner, the left and right side walls of the cultivating box (2) are both vertically provided with sliding openings (22) communicated with the cultivating groove (3), two soil compacting components are fixedly mounted on the surfaces of the second sliding blocks (21) close to each other, the two soil compacting components respectively pass through the two sliding openings (22) to enter the cultivating groove (3), and the top end of the second threaded rod (20) penetrates out of the mounting shell (19) and is fixedly provided with a second adjusting wheel (23).

7. The ginger virus-free asexual propagation device according to claim 6, wherein the soil compacting assembly comprises a mounting plate (24), a spring rod (25) and a pressing plate (26), the mounting plate (24) is fixedly connected with the side wall of the second sliding block (21), the lower surface of the mounting plate is vertically and fixedly connected with the spring rod (25), and the pressing plate (26) is fixedly connected with one end of the spring rod (25) far away from the mounting plate (24).

8. The ginger detoxification asexual propagation device according to claim 1, wherein a sliding groove (27) is formed in the lower surface of the mounting plate (24), a sliding plate (28) is slidably mounted in the sliding groove (27), and a pull rod (29) is fixedly mounted on the surfaces, close to each other, of the two sliding plates (28).

9. The ginger virus-free asexual propagation device according to claim 1, wherein a first magnetic block (30) is fixedly installed at the bottom of the inner groove of the clamping groove (6), a second magnetic block (31) is fixedly installed at one end of each of the two clamping rods (9) close to each other, and the magnetic poles of the surfaces of the first magnetic block (30) and the second magnetic block (31) close to each other are opposite.

10. A virus-free asexual propagation method for ginger is characterized by comprising the following steps: