CN112920932A - Consumable fixing device for nucleic acid extraction and using method thereof - Google Patents
Consumable fixing device for nucleic acid extraction and using method thereof Download PDFInfo
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- CN112920932A CN112920932A CN202110288441.6A CN202110288441A CN112920932A CN 112920932 A CN112920932 A CN 112920932A CN 202110288441 A CN202110288441 A CN 202110288441A CN 112920932 A CN112920932 A CN 112920932A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 48
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 47
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 47
- 238000000605 extraction Methods 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000010828 elution Methods 0.000 claims description 31
- 230000009089 cytolysis Effects 0.000 claims description 22
- 238000005336 cracking Methods 0.000 claims description 16
- 238000007789 sealing Methods 0.000 claims description 15
- 239000011324 bead Substances 0.000 claims description 14
- 239000011148 porous material Substances 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 7
- 239000012149 elution buffer Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 239000011534 wash buffer Substances 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 2
- 239000006166 lysate Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 66
- 210000002421 cell wall Anatomy 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 13
- 125000006850 spacer group Chemical group 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- Zoology (AREA)
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- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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Abstract
The invention provides a consumable fixing device for nucleic acid extraction and a use method thereof, relating to the technical field of consumable tube fixing and comprising the following steps: a consumable rack and a mounting seat; through the consumptive material arch on the consumptive material subassembly and the buckle on the consumptive material frame, with consumptive material subassembly joint on the consumptive material frame, make things convenient for the dismantlement of consumptive material subassembly on the consumptive material frame, and simultaneously, through the elastic component on the mount pad cell wall and the bellying cooperation on the consumptive material frame, with consumptive material frame joint on the mount pad, make things convenient for plug at any time between consumptive material frame and the mount pad, it directly places the porous single tube consumptive material that exists among the prior art and has fixed insecure on nucleic acid extraction module mount pad, get the technical problem of putting the difficulty.
Description
Technical Field
The invention relates to the technical field of consumable tube fixing, in particular to a consumable fixing device for nucleic acid extraction and a using method thereof.
Background
During nucleic acid extraction, porous single tube consumptive material can only be got one and put, gets to put slower, influences the efficiency of work, and the porous single tube consumptive material of prior art directly places on nucleic acid extraction module mount pad, and fixed knot that every porous single tube consumptive material and nucleic acid extraction between the module mount pad has fixed insecure, gets the problem of putting the difficulty.
Disclosure of Invention
The invention aims to provide a consumable fixing device for nucleic acid extraction and a use method thereof, so as to solve the technical problems that porous single-tube consumables are not firmly fixed and are difficult to take and place when being directly placed on a nucleic acid extraction module mounting seat in the prior art.
In a first aspect, the present invention provides a consumable fixing device for nucleic acid extraction, for fixing a consumable component, comprising: a consumable rack and a mounting seat;
the consumable rack is provided with a buckle, a consumable bulge is arranged on the consumable component, and the consumable bulge is clamped with the buckle;
the mounting seat is provided with the fixed slot, be provided with the elastic component on the inner wall of fixed slot, be provided with the bellying on the consumptive material frame, the elastic component configure into can with the bellying joint, with will the consumptive material frame is fixed in the fixed slot.
In an alternative embodiment, the consumable fixing device for nucleic acid extraction further comprises a plunger fixing seat;
the plunger fixing seats are arranged in plurality, the plunger fixing seats are all arranged on the mounting seat, and the elastic piece is mounted on the plunger fixing seats;
the consumptive material frame is provided with a plurality ofly, and is a plurality of the elastic component can the joint be a plurality of the consumptive material frame.
In an optional embodiment, the consumable rack is provided with a plurality of placing parts for placing consumable components, and a grid is arranged between any two adjacent placing parts;
each placing part is provided with a sheath hole position groove, an elution hole position groove, a connecting hole position groove and a cracking hole position groove.
In an optional embodiment, a plurality of buckles are arranged on the consumable rack, a spacing block is arranged between any two adjacent buckles, and a slit is arranged between any adjacent buckle and the spacing block;
the outer wall of the consumable rack is provided with a handheld bulge.
In an alternative embodiment, the consumable assembly has a lysis chamber, an elution chamber, and a plurality of wells;
the distance between the cracking cavity and the adjacent connecting cavity is larger than the distance between any two adjacent connecting cavities;
the distance between the elution cavity and the adjacent pore connecting cavity is larger than the distance between any two adjacent pore connecting cavities.
In an alternative embodiment, the lysis chamber, the elution chamber and the pore chamber are each of an opening diameter size greater than an end diameter size.
In an alternative embodiment, the top opening positions of the lysis cavity, the elution cavity and the pore connecting cavity are provided with membrane sealing steps.
In an alternative embodiment, the consumable assembly further has a sheath cavity in which the sheath body can be placed, the top opening of the sheath body being provided with a sheath groove.
In a second aspect, the present invention provides a method for using a consumable fixing device for nucleic acid extraction, comprising the steps of:
injecting lysis solution into the lysis cavity, injecting washing buffer solution and magnetic beads into the multiple connected-pore cavities respectively, and injecting elution buffer solution into the elution cavity;
the consumable bulge is clamped with the buckle so as to clamp the consumable assembly on the consumable rack;
and sealing the membrane of the consumable component.
In an alternative embodiment, the method further comprises the following steps:
the elastic piece is clamped with the protruding part so as to fix the consumable rack bearing the consumable component on the mounting seat;
removing the sealing film of the consumable component;
placing the sheath main body in a sheath cavity, and injecting a sample to be detected into the cracking cavity;
adsorbing the magnetic beads on the sheath main body;
extending the sheath main body with the magnetic beads into the cracking cavity;
the sheath body is subjected to washing and elution of nucleic acids.
The invention provides a consumable fixing device for nucleic acid extraction, which is used for fixing a consumable component and comprises: a consumable rack and a mounting seat; the consumable rack is provided with a buckle, a consumable bulge is arranged on the consumable component, and the consumable bulge is clamped with the buckle; the mount pad is provided with the fixed slot, is provided with the elastic component on the inner wall of fixed slot, is provided with the bellying on the consumptive material frame, and the elastic component configuration is configured to can with the bellying joint to be fixed in the fixed slot with the consumptive material frame. Through the consumptive material arch on the consumptive material subassembly and the buckle on the consumptive material frame, with consumptive material subassembly joint on the consumptive material frame, make things convenient for the dismantlement of consumptive material subassembly on the consumptive material frame, and simultaneously, through the elastic component on the mount pad cell wall and the bellying cooperation on the consumptive material frame, with consumptive material frame joint on the mount pad, make things convenient for plug at any time between consumptive material frame and the mount pad, it directly places the porous single tube consumptive material that exists among the prior art and has fixed insecure on nucleic acid extraction module mount pad, get the technical problem of putting the difficulty.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram illustrating an exploded structure of a consumable fixing device for nucleic acid extraction according to an embodiment of the present invention;
FIG. 2 is a sectional view of the structure of a consumable rack in the consumable fixing device for nucleic acid extraction according to the embodiment of the present invention;
FIG. 3 is a schematic diagram illustrating an overall structure of a consumable fixing device for nucleic acid extraction according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of a consumable rack in the consumable fixing device for nucleic acid extraction according to the embodiment of the present invention;
FIG. 5 is a schematic structural view of a consumable component in the consumable fixing device for nucleic acid extraction according to the embodiment of the present invention.
Icon: 100-consumable racks; 110-buckle; 120-a boss; 130-a grid; 140-sheath hole position groove; 150-elution pore site groove; 160-a hole position groove with holes; 170-lysis pore site groove; 180-spacer blocks; 190-a hand-held projection; 200-a mounting seat; 210-an elastic member; 220-plunger fixing seat; 300-consumable components; 310-consumable bumps; 320-a lysis chamber; 330-elution chamber; 340-a connecting cavity; 350-sealing a film step; 360-sheath lumen; 370-sheath groove.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships that the products of the present invention are conventionally placed in use, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Some embodiments of the invention are described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
As shown in FIG. 1, FIG. 2 and FIG. 3, the consumable fixing device for nucleic acid extraction according to the present embodiment is used to fix a consumable assembly 300, and comprises: a consumable rack 100 and a mounting base 200; the consumable rack 100 is provided with a buckle 110, the consumable component 300 is provided with a consumable protrusion 310, and the consumable protrusion 310 is clamped with the buckle 110; the mounting seat 200 is provided with a fixing groove, an elastic member 210 is provided on an inner wall of the fixing groove, a protrusion 120 is provided on the consumable rack 100, and the elastic member 210 is configured to be capable of being engaged with the protrusion 120 to fix the consumable rack 100 in the fixing groove.
Specifically, both sides position at consumable component 300 all is provided with the protruding 310 of consumptive material, consumable frame 100 is hollow structure, consumable component 300 can stretch into consumable frame 100, and inner wall position at consumable frame 100 sets up buckle 110, the specific quantity of buckle 110 and the protruding 310 of consumptive material one-to-one, when consumable component 300 stretches into consumable frame 100, the protruding 310 of consumptive material and buckle 110 joint, consumable component 300 fixes on consumable frame 100, form the consumable frame 100 that has consumable component 300, later will have the consumptive material frame 100 of consumable component 300 to stretch into the fixed slot of mount pad 200, elastic component 210 on the fixed slot cell wall contacts with bellying 120 on the consumable frame 100, with the joint of consumable frame 100 on the fixing base, accomplish the fixed of consumable component 300. Elastic component 210 specifically can set up to convex elasticity plunger, and convex elasticity plunger has certain elasticity, and when consumptive material frame 100 stretched into mount pad 200, bellying 120 extruded the elasticity plunger and warp, and behind the position of bellying 120 crossed the elasticity plunger, the elasticity plunger recovered original shape because of elasticity, and then with bellying 120 joint on the elasticity plunger, and because of the elastic action, can fix fast or dismantle consumptive material frame 100.
The nucleic acid extraction that this embodiment provided is with consumptive material fixing device for fixed consumptive material subassembly 300 includes: a consumable rack 100 and a mounting base 200; the consumable rack 100 is provided with a buckle 110, the consumable component 300 is provided with a consumable protrusion 310, and the consumable protrusion 310 is clamped with the buckle 110; the mounting seat 200 is provided with a fixing groove, an elastic member 210 is provided on an inner wall of the fixing groove, a protrusion 120 is provided on the consumable rack 100, and the elastic member 210 is configured to be capable of being engaged with the protrusion 120 to fix the consumable rack 100 in the fixing groove. Through the protruding 310 of consumptive material on the consumptive material subassembly 300 and the buckle 110 on the consumptive material frame 100, with consumptive material subassembly 300 joint on consumptive material frame 100, make things convenient for the dismantlement of consumptive material subassembly 300 on consumptive material frame 100, and simultaneously, elastic component 210 through on the mount pad 200 cell wall cooperates with bellying 120 on the consumptive material frame 100, with consumptive material frame 100 joint on mount pad 200, make things convenient for plug at any time between consumptive material frame 100 and the mount pad 200, it directly places to have on nucleic acid extraction module mount pad 200 and fixes insecure to have alleviated the porous single tube consumptive material that exists among the prior art, get and put the technical problem of difficulty.
On the basis of the above embodiments, in an optional implementation manner, the consumable fixing device for nucleic acid extraction provided by this embodiment further includes a plunger fixing seat 220; a plurality of plunger fixing seats 220 are arranged, the plunger fixing seats 220 are all arranged on the mounting seat 200, and the elastic part 210 is arranged on the plunger fixing seats 220; the consumable rack 100 is provided with a plurality of elastic members 210 capable of clamping the plurality of consumable racks 100.
Specifically, mount pad 200 can hold a plurality of consumptive material frame 100, set up a plurality of plunger fixing base 220 on mount pad 200, the specific quantity of plunger fixing base 220 is decided according to the quantity of consumptive material frame 100, guarantee that at least one consumptive material frame 100 corresponds and set up two plunger fixing base 220, two plunger fixing base 220 are located the both sides of consumptive material frame 100 respectively, and, all set up an elastic component 210 on every plunger fixing base 220 at least, select the mounted position and the quantity of plunger fixing base 220 according to actual conditions.
As shown in fig. 4, in an alternative embodiment, the consumable rack 100 is provided with a plurality of placing parts for placing the consumable components 300, and a grid 130 is arranged between any two adjacent placing parts; each placing part is provided with a sheath hole site groove 140, an elution hole site groove 150, a connecting hole site groove 160 and a cracking hole site groove 170.
Specifically, consumable component 300 can set up to porous single tube consumptive material, and porous single tube consumptive material can be provided with a plurality ofly, and consumable frame 100 has a plurality of portions of placing, and a porous single tube consumptive material all can be placed to every portion of placing, separates through grid 130 between two portions of placing, and consumable component 300 stretches into in sheath hole site groove 140, elution hole site groove 150, even hole site groove 160 and the schizolysis hole site groove 170.
The use of consumptive material frame 100 is passed through to this embodiment, has realized once only getting when the porous single tube consumptive material of multiunit separation originally, has solved among the prior art can only one get and has put, gets the technical problem that puts slower, has improved the flux of getting and has put efficiency, and wherein each porous single tube consumptive material on the consumptive material frame 100 can also be convenient get from consumptive material frame 100 and put, possesses oneself and gets the degree of freedom.
In an alternative embodiment, the consumable rack 100 is provided with a plurality of buckles 110, a spacer block 180 is arranged between any two adjacent buckles 110, and a slit is arranged between any adjacent buckle 110 and spacer block 180; the outer wall of the consumable rack 100 is provided with a handheld protrusion 190.
Specifically, set up a plurality of buckles 110 on the consumptive material frame 100 both sides, set up spacer 180 between the buckle 110, buckle 110 and spacer 180 interval set up, set up the slit between buckle 110 and the spacer 180, the deformation space of buckle 110 on the slit can be left out consumptive material frame 100 lateral wall, be convenient for on consumptive material subassembly 300 block to consumptive material frame 100. The buckle 110 is arranged on the end side of the consumable rack 100 corresponding to the consumable assembly 300; the spacing block 180 is disposed on the end side of the consumable rack 100 corresponding to the grid 130; the rear part of the spacing block 180 is provided with a reinforcing rib for reinforcing the strength of the consumable rack 100; the upper sides of the three spacing blocks 180 at the middle part are designed into handheld protrusions 190 protruding outwards, so that the consumable rack 100 can be conveniently taken and placed by hands.
In an alternative embodiment, the consumable assembly 300 has a lysis chamber 320, an elution chamber 330, and a plurality of communication chambers 340; the distance between the cracking cavity 320 and the adjacent connecting hole cavity 340 is larger than the distance between any two adjacent connecting hole cavities 340; the distance between elution chamber 330 and adjacent well bore 340 is greater than the distance between any adjacent two well bore 340.
Specifically, the consumable assembly 300 has a plurality of cavities, wherein the lysis chamber 320 is used for heating and lysis during nucleic acid extraction, and the elution chamber 330 is used for heating and elution during nucleic acid extraction, and because the heating modules are arranged below the elution chamber 330 and the lysis chamber 320, the interference of heat radiation to the through-hole chamber 340 can be reduced through the arrangement of the distance.
In an alternative embodiment, the lysis chamber 320, elution chamber 330, and well chamber 340 are all open diameter sizes that are larger than the end diameter sizes.
Specifically, each cavity of the consumable assembly 300 is provided with a structure with a thick upper surface and a thin lower surface, the sheath body is matched with the magnetic rod in nucleic acid extraction, cracking, washing, elution and other steps need to be carried out in each hole site, the lower surface is designed to be thin and can be adapted to the size of the end part of the sheath body, if the part is designed to be thick and the space is large, part of magnetic beads can not be absorbed onto the surface of the sheath body due to insufficient magnetic force of the magnetic rod, on the other hand, nucleic acid cracking and extraction caused by nucleic acid residue are prevented from being insufficient, the thick part designed on the upper surface can increase the volume of the hole site, the cavity space is larger, more samples can be received, and the sample capacity is increased.
In an alternative embodiment, the top opening positions of the lysis chamber 320, the elution chamber 330 and the pore connecting chamber 340 are all provided with a sealing step 350.
Specifically, the sealing film step 350 is arranged at the opening positions of the cracking cavity 320, the elution cavity 330 and the connecting hole cavity 340, so that the sealing film sealing effect and the sealing film attractiveness are improved.
In an alternative embodiment, as shown in fig. 5, the consumable assembly 300 further has a sheath cavity 360 in which the sheath body can be placed, the top opening of the sheath body being provided with a sheath groove 370.
Specifically, a circle of sheath grooves 370 are formed in the sheath body, protrusions are correspondingly formed in the sheath plug, and the sheath body can be conveniently taken and placed through the cooperation of the protrusions in the sheath plug and the sheath grooves 370 in the sheath body.
The application method of the consumable fixing device for nucleic acid extraction provided by the embodiment comprises the following steps: injecting lysis solution into the lysis cavity 320, injecting washing buffer solution and magnetic beads into the plurality of pore connecting cavities 340 respectively, and injecting elution buffer solution into the elution cavity 330; the consumable protrusion 310 is clamped with the clamp 110 to clamp the consumable assembly 300 on the consumable rack 100; the consumable assembly 300 is subjected to a sealing process.
Specifically, inject the lysate into the schizolysis chamber 320 of consumable component 300, inject washing buffer and magnetic bead into in even hole chamber 340 respectively, inject elution buffer into in elution chamber 330, place consumable component 300 block on consumable frame 100, seal the membrane together with consumable component 300 on the consumable frame 100.
In an alternative embodiment, the method further comprises the following steps: the elastic member 210 is clamped with the boss 120 to fix the consumable rack 100 carrying the consumable assembly 300 on the mounting seat 200; releasing the sealing film of the consumable assembly 300; placing the sheath body in the sheath cavity 360, and injecting a sample to be detected in the lysis cavity 320; adsorbing the magnetic beads on the sheath main body; extending the sheath body with the magnetic beads into the lysis chamber 320; the sheath body is subjected to washing and elution of nucleic acids.
Specifically, one consumable rack 100 carrying a plurality of sets of consumable components 300 is clamped with the mounting seat 200; one consumable rack 100 carrying a plurality of sets of consumable components 300 and a sheath body is engaged with the mount 200 as needed. Removing the sealing film of the consumable assembly 300 on the consumable rack 100; placing the sheath body in the sheath cavity 360 of the consumable assembly 300; injecting a sample to be tested into the lysis chamber 320; the next nucleic acid extraction procedure is then started: the sheath main body is sleeved on a sheath plug on a magnetic rod of the nucleic acid extraction module through a sheath groove 370, and the sheath main body absorbs magnetic beads from the magnetic bead placing cavity and injects the magnetic beads into the lysis cavity 320; in the cracking chamber 320, the sheath body and the magnetic rod start to vibrate and crack; after the cracking is completed, the sheath main body and the magnetic rod are synchronously moved out of the cracking cavity 320 and synchronously sleeved in the washing cavity for washing nucleic acid, and after the four washing cavities are washed, the sheath main body and the magnetic rod are synchronously sleeved in the elution cavity 330 of the consumable for eluting nucleic acid.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A consumable fixing device for nucleic acid extraction for fixing a consumable component (300), characterized by comprising: a consumable rack (100) and a mounting seat (200);
the consumable rack (100) is provided with a buckle (110), the consumable component (300) is provided with a consumable bulge (310), and the consumable bulge (310) is clamped with the buckle (110);
the mounting seat (200) is provided with a fixing groove, an elastic piece (210) is arranged on the inner wall of the fixing groove, a protruding portion (120) is arranged on the consumable rack (100), the elastic piece (210) is configured to be capable of being connected with the protruding portion (120) in a clamping mode, and therefore the consumable rack (100) is fixed in the fixing groove.
2. The nucleic acid extraction consumable fixing device according to claim 1,
the consumable fixing device for nucleic acid extraction further comprises a plunger fixing seat (220);
a plurality of plunger fixing seats (220) are arranged, the plunger fixing seats (220) are all arranged on the mounting seat (200), and the elastic piece (210) is mounted on the plunger fixing seats (220);
the consumable rack (100) is provided with a plurality of elastic pieces (210) which can be clamped with the consumable rack (100).
3. The nucleic acid extraction consumable fixing device according to claim 1,
the consumable rack (100) is provided with a plurality of placing parts for placing the consumable components (300), and a grid (130) is arranged between any two adjacent placing parts;
each placing part is provided with a sheath hole site groove (140), an elution hole site groove (150), a connecting hole site groove (160) and a cracking hole site groove (170).
4. The nucleic acid extraction consumable fixing device according to claim 1,
the consumable rack (100) is provided with a plurality of buckles (110), a spacing block (180) is arranged between any two adjacent buckles (110), and a slit is arranged between any adjacent buckle (110) and the spacing block (180);
the outer wall of the consumable rack (100) is provided with a handheld bulge (190).
5. The nucleic acid extraction consumable fixing device according to claim 1,
the consumable assembly (300) having a lysis chamber (320), an elution chamber (330), and a plurality of communication chambers (340);
the distance between the cracking cavity (320) and the adjacent connecting cavity (340) is larger than the distance between any two adjacent connecting cavities (340);
the distance between the elution chamber (330) and the adjacent pore connecting chamber (340) is larger than the distance between any two adjacent pore connecting chambers (340).
6. The nucleic acid extraction consumable fixing device according to claim 5,
the lysis chamber (320), the elution chamber (330) and the pore connecting chamber (340) are all of an opening diameter size larger than an end diameter size.
7. The nucleic acid extraction consumable fixing device according to claim 5,
and membrane sealing steps (350) are arranged at the top opening positions of the cracking cavity (320), the elution cavity (330) and the pore connecting cavity (340).
8. The nucleic acid extraction consumable fixing device according to claim 5,
the consumable assembly (300) further has a sheath cavity (360) in which a sheath body can be placed, and a sheath groove (370) is provided at a top opening of the sheath body.
9. A method for using a consumable fixing device for nucleic acid extraction according to any one of claims 1 to 8, comprising the steps of:
lysate is injected into the lysis cavity (320), washing buffer and magnetic beads are respectively injected into the multiple connected cavity (340), and elution buffer is injected into the elution cavity (330);
the consumable protrusion (310) is clamped with the clamp (110) to clamp the consumable assembly (300) on the consumable frame (100);
and performing film sealing treatment on the consumable component (300).
10. The method of using the consumable fixing device for nucleic acid extraction according to claim 9, further comprising the steps of:
the elastic piece (210) is clamped with the lug boss (120) so as to fix the consumable rack (100) bearing the consumable component (300) on the mounting seat (200);
removing the sealing film of the consumable component (300);
placing the sheath body in a sheath cavity (360), and injecting a sample to be detected in the cracking cavity (320);
adsorbing the magnetic beads on the sheath main body;
extending the sheath body with the magnetic beads into a lysis chamber (320);
the sheath body is subjected to washing and elution of nucleic acids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110288441.6A CN112920932B (en) | 2021-03-17 | 2021-03-17 | Consumable fixing device for nucleic acid extraction and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110288441.6A CN112920932B (en) | 2021-03-17 | 2021-03-17 | Consumable fixing device for nucleic acid extraction and use method thereof |
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| Publication Number | Publication Date |
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| CN112920932A true CN112920932A (en) | 2021-06-08 |
| CN112920932B CN112920932B (en) | 2023-10-27 |
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| CN202110288441.6A Active CN112920932B (en) | 2021-03-17 | 2021-03-17 | Consumable fixing device for nucleic acid extraction and use method thereof |
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| CN (1) | CN112920932B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120115238A1 (en) * | 2009-07-09 | 2012-05-10 | Toppan Printing Co., Ltd. | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
| CN106859507A (en) * | 2016-11-23 | 2017-06-20 | 德阳九鼎智远知识产权运营有限公司 | A kind of sweeping robot |
| CN207456907U (en) * | 2017-10-17 | 2018-06-05 | 深圳市易瑞生物技术股份有限公司 | Eight connecting leg magnetic frame of eight connecting leg frame of modularization, modularization support base and modularization |
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2021
- 2021-03-17 CN CN202110288441.6A patent/CN112920932B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120115238A1 (en) * | 2009-07-09 | 2012-05-10 | Toppan Printing Co., Ltd. | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
| CN106859507A (en) * | 2016-11-23 | 2017-06-20 | 德阳九鼎智远知识产权运营有限公司 | A kind of sweeping robot |
| CN207456907U (en) * | 2017-10-17 | 2018-06-05 | 深圳市易瑞生物技术股份有限公司 | Eight connecting leg magnetic frame of eight connecting leg frame of modularization, modularization support base and modularization |
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| CN112920932B (en) | 2023-10-27 |
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