CN112920235A - Preparation method of isomalt - Google Patents

Preparation method of isomalt Download PDF

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Publication number
CN112920235A
CN112920235A CN202110129658.2A CN202110129658A CN112920235A CN 112920235 A CN112920235 A CN 112920235A CN 202110129658 A CN202110129658 A CN 202110129658A CN 112920235 A CN112920235 A CN 112920235A
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isomaltulose
isomaltitol
isomalt
sucrose
solution
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闫传浩
刘建
任尚美
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Shandong Jianyihong Biopharmaceutical Co ltd
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Shandong Jianyihong Biopharmaceutical Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/02—Acyclic radicals, not substituted by cyclic structures
    • C07H15/04—Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/04—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides

Abstract

The invention provides a preparation method of isomaltitol, which comprises the following steps: (1) preparing isomaltulose by sucrose conversion; (2) carrying out decolorization, ion exchange and evaporation concentration on the isomaltulose liquid; (3) preparing isomaltitol by hydrogenation reduction of isomaltulose; (4) separating the isomalt by chromatography; (5) concentrating the isomaltulose alcohol component and preserving the heat of the isomaltulose alcohol component; (6) isomalt crystallization; (7) cooling, crushing and screening the crystallized isomaltulose alcohol; (8) the fusel fraction is subjected to ion exchange and concentration by evaporation. The invention saves the steam consumption in the isomaltulose evaporation process, omits the processes of isomaltulose crystallization, centrifugation and water addition dissolution, simplifies the production process, reduces the production cost, and leads the target product isomaltulose and the by-products sorbitol and mannitol obtained after hydrogenation to be easy to separate compared with the substances before hydrogenation.

Description

Preparation method of isomalt
Technical Field
The invention relates to the field of food preparation, in particular to a method for preparing isomaltitol.
Background
Isomalt (Isomalt), also known as palatinol or Isomalt, is a mixture of two isomers, alpha-D-glucopyranosyl-1, 6-D-sorbitol (1,6-GPS) and alpha-D-glucopyranosyl-1, 1-D-mannitol dihydrate (1,1-GPM dihydrate), respectively. The isomaltitol is a functional sugar alcohol with low calorie and low sweetness, is not easy to absorb moisture, has the melting point of 145-150 ℃, does not cause decayed teeth or increase blood sugar, and is a sweetener suitable for diabetics.
At present, the conventional production process of isomaltulose is to produce crystalline isomaltulose, dissolve the isomaltulose with water, then carry out hydrogenation reduction, and then recrystallize to prepare isomaltulose, the method increases the processes of evaporation, crystallization, centrifugation and dissolution with water of isomaltulose, not only the process is complicated, but also the production cost is increased, meanwhile, the isomaltulose crystallization process can produce a large amount of mother liquor, and the mother liquor comprises the following components: 40-50% of isomaltulose, 33-40% of trehalulose, 5-9% of fructose, 2-4% of glucose and a small amount of impurity sugar, and the mother liquor cannot be sold as a product because of no relevant product standard. In addition, the two main components of isomaltulose and trehalulose are difficult to separate, so that the mother liquor of this part is difficult to be utilized with high added value.
In order to solve the above problems, the CN200510035439.9 patent uses immobilized yeast to remove the impurity sugar other than isomaltulose in the transformation liquid, and the transformation liquid has the following composition: 73-80% of isomaltulose, 13-18% of trehalulose, 2-4% of fructose and about 1% of glucose, wherein the impurity sugar except the isomaltulose is removed by a fermentation method, so that more than 20% of sugar is fermented, which is a great waste. In addition, since fermentation requires the sugar concentration of the conversion solution to be low, below 25%, the concentration of the conversion solution needs to be controlled, which increases the amount of steam used for subsequent evaporative concentration, thus increasing the production cost.
How to solve the above technical problems is the subject of the present invention.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a method for preparing isomaltitol by directly hydrogenating to prepare isomaltitol without evaporative crystallization, then carrying out chromatographic purification on the obtained isomaltitol by a simulated moving bed to obtain an isomaltitol component and a fusel component, and crystallizing the isomaltitol component to obtain an isomaltitol product.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention provides a preparation method of isomaltitol, which comprises the following steps:
(1) sucrose conversion to isomaltulose: preparing sucrose into a sucrose solution with a certain concentration, wherein the concentration of the sucrose solution is 20-55%, adding the sucrose solution into an immobilized bacterium with sucrose isomerase, the weight of the immobilized bacterium is 8-12% of the weight of the sucrose solution, converting sucrose into isomaltulose under the conditions of pH5.0-8.0, temperature of 25-32 ℃, introducing sterile compressed air and pressure of 0.03-0.06Mpa, and obtaining isomaltulose liquid;
the isomaltulose solution had the following composition: 73-80% of isomaltulose, 13-18% of trehalulose, 2-4% of fructose and 0.5-2% of glucose.
(2) Carrying out decoloration, ion exchange and evaporation concentration on the isomaltulose liquid obtained in the step (1) to obtain isomaltulose purified liquid;
since the decolorization, ion exchange and evaporation concentration in step (2) are only to remove color, ions and moisture in the sugar solution, and have no influence on the sugar composition, the composition of the isomaltulose purification solution is the same as that of the isomaltulose sugar solution in step (1), and the composition of the isomaltulose purification solution is also: 73-80% of isomaltulose, 13-18% of trehalulose, 2-4% of fructose and 0.5-2% of glucose.
(3) Hydrogenation reduction of isomaltulose to isomalt: adjusting the pH of the isomaltulose purified liquid obtained in the step (2) to 6.5-8.5, and carrying out catalytic hydrogenation to obtain isomaltulose liquid;
the catalytic hydrogenation conditions are as follows: the concentration of the material is 30-50%, the catalyst is Raney nickel, the addition amount of the Raney nickel is 5-15% of the dry basis of the material, the temperature is 100-130 ℃, and the pressure is 5-13 Mpa;
the isomaltulose alcohol solution comprises the following components: 84-92% of isomalt, 6-10% of sorbitol and 1-2% of mannitol;
when the isomaltulose purification solution is hydrogenated and reduced, isomaltulose is reduced to obtain isomaltitol, a trehalulose reduction product is also isomaltitol through detection and structural analysis, a byproduct trehalulose is fully utilized, and the content of isomaltitol is slightly lower than the sum of the contents of isomaltulose and trehalulose because of the hydrogenation and reduction side reaction.
(4) Isomalt chromatography: carrying out ion exchange on the isomaltitol solution obtained in the step (3) to remove ions, and then carrying out simulated moving bed chromatographic purification to obtain an isomaltitol component and a fusel component;
the simulated moving bed chromatographic purification conditions are as follows: eluent is water, separating agent is calcium type strong acid cation exchange resin, the content of solid matters in the feed is 30-60%, and the separation temperature is 50-80 ℃;
the obtained isomaltulose alcohol component contains isomaltitol more than 97%, sorbitol 50-57%, isomaltitol 28-35%, and mannitol 15-22%.
(5) And (3) primarily concentrating the isomaltulose alcohol component obtained in the step (4) by adopting an evaporator, continuously concentrating by adopting a vacuum scraper evaporator until the solid content is more than 90 percent to obtain molten isomaltulose, and preserving the heat of the molten isomaltulose at the temperature of 125-150 ℃.
(6) Isomalt crystallization: spraying the heat-preserved molten isomaltitol obtained in the step (5) onto the suspended isomaltitol seed crystal through a nozzle, and stirring while spraying to crystallize the isomaltitol;
the spraying is realized by hot compressed air, the temperature of the hot air is 125-150 ℃, and the pressure is 0.4-0.7 Mpa;
the isomalt seed in suspension is achieved by: putting isomaltitol seed crystals into a crystallizer in advance, stirring by using a double-helix stirrer, lifting the materials upwards by the self-transmission of the helix, and making the materials move horizontally by revolution, thereby achieving the purpose of suspending the materials;
the mass ratio of the molten isomaltitol to the suspended isomaltitol seed crystal is (1-2): (1-2).
(7) And (4) cooling, drying, crushing and screening the crystallized isomaltitol obtained in the step (6) to obtain an isomaltitol product.
(8) And (4) carrying out ion exchange and evaporation concentration on the fusel component obtained in the step (4) to obtain a liquid sugar alcohol product.
The invention has the beneficial effects that:
1. the steam consumption in the isomaltulose evaporation process is saved, and the production cost is reduced.
2. The procedures of isomaltulose crystallization, centrifugation and water addition dissolution are omitted, the production process is simplified, and the production cost is reduced.
3. The target product isomaltitol is obtained by hydrogenation reduction of the byproduct trehalulose, and the product yield is improved.
4. The target product isomaltitol and by-products sorbitol and mannitol obtained after hydrogenation are easier to separate than the material before hydrogenation.
Drawings
FIG. 1 is a diagram showing the separation of isomaltulose from a calcium-type strongly acidic cation exchange resin according to an embodiment of the present invention.
FIG. 2 is a diagram showing the separation of isomaltitol solution by a calcium-type strongly acidic cation exchange resin according to an embodiment of the present invention.
Detailed Description
Technical characteristics of the scheme can be clearly explained, and the scheme is explained through a specific implementation mode.
The first embodiment is as follows:
this example is a process for the preparation of isomalt comprising the steps of:
(1) sucrose conversion to isomaltulose: preparing sucrose into a sucrose solution, adjusting the concentration of the sucrose solution to be 45%, adjusting the pH value to be 6.5, adding the sucrose solution into an immobilized bacterium with sucrose isomerase, wherein the weight of the immobilized bacterium is 10% of the weight of the sucrose solution, the temperature is 28 ℃, introducing sterile compressed air, and converting sucrose into isomaltulose under the condition of 0.05Mpa to obtain isomaltulose liquid;
the isomaltulose solution had the following composition: 79.5 percent of isomaltulose, 14.7 percent of trehalulose, 3.2 percent of fructose and 0.9 percent of glucose.
(2) Carrying out decoloration, ion exchange and evaporation concentration on the isomaltulose liquid obtained in the step (1) to obtain isomaltulose purified liquid;
the isomaltulose purifying liquid comprises the following components: 79.5 percent of isomaltulose, 14.7 percent of trehalulose, 3.2 percent of fructose and 0.9 percent of glucose.
(3) Hydrogenation reduction of isomaltulose to isomalt: adjusting the pH value of the isomaltulose purified liquid obtained in the step (2) to 7.5 for catalytic hydrogenation to obtain isomaltulose liquid;
the catalytic hydrogenation conditions are as follows: the concentration of the material is 40 percent, the catalyst is Raney nickel, the addition amount of the Raney nickel is 10 percent of the dry basis of the material, the temperature is 100 ℃, the pressure is 9Mpa, and the reaction is stopped when the conversion rate reaches more than 98 percent;
the isomaltulose alcohol solution comprises the following components: isomalt 90.6%, sorbitol 6.5%, mannitol 1.2%;
(4) isomalt chromatography: carrying out ion exchange on the isomaltitol solution obtained in the step (3) to remove ions, and then carrying out simulated moving bed chromatographic purification to obtain an isomaltitol component and a fusel component;
the simulated moving bed chromatographic purification conditions are as follows: eluent is water, separating agent is calcium type strong acid cation exchange resin, the content of solid matters in the feed is 40%, and the separation temperature is 65 ℃;
the isomaltulose alcohol component obtained contained 99.2% isomalt, the sorbitol component contained 52.7%, isomalt 29% and mannitol 18%.
(5) And (3) primarily concentrating the isomaltulose alcohol component obtained in the step (4) by adopting an evaporator, continuously concentrating by adopting a vacuum scraper evaporator until the solid content is more than 90 percent to obtain molten isomaltulose alcohol, and preserving the heat of the molten isomaltulose alcohol at the temperature of 130 ℃.
(6) Isomalt crystallization: spraying the heat-preserved molten isomaltitol obtained in the step (5) onto the suspended isomaltitol seed crystal through a nozzle, and stirring while spraying to crystallize the isomaltitol;
the spraying is realized by hot compressed air, the temperature of the hot air is 145 ℃, and the pressure is 0.5 Mpa;
the mass ratio of the molten isomalt to the suspended isomalt seed crystal was 1: 1.
(7) And (4) cooling, drying, crushing and screening the crystallized isomaltitol obtained in the step (6) to obtain an isomaltitol product.
(8) And (4) carrying out ion exchange and evaporation concentration on the fusel component obtained in the step (4) to obtain a liquid sugar alcohol product.
Separating the isomaltulose from the isomaltitol solution by using a calcium type strong-acid cation exchange resin:
table 1: FIG. 1 is a table of retention time versus substance
Retention time (min) 14.994 15.607 17.787 22.294
Name of substance Glucose Isomaltulose Trehalulose Fructose
Table 2: FIG. 2 is a table of retention time versus substance
Retention time (min) 17.746 22.902 25.849 35.180
Name of substance Isomalt A Isomalt B Mannitol Sorbitol
As can be seen from tables 1 and 2, the calcium-type strongly acidic cation exchange resin has a better effect of separating each substance in isomaltulose solution than isomaltulose, and therefore the resin is easier to separate isomaltulose and sorbitol and mannitol, which are the target products, obtained after hydrogenation, than the substances before hydrogenation.
Example two:
this example is a process for the preparation of isomalt comprising the steps of:
(1) sucrose conversion to isomaltulose: preparing sucrose into a sucrose solution, adjusting the pH value to 8.0, adding the sucrose solution into an immobilized bacterium with sucrose isomerase, wherein the weight of the immobilized bacterium is 12% of the weight of the sucrose solution, the temperature is 31 ℃, introducing sterile compressed air, and converting sucrose into isomaltulose under the condition of 0.06MPa of pressure to obtain isomaltulose liquid;
the isomaltulose solution had the following composition: 73.2% of isomaltulose, 17.4% of trehalulose, 3.8% of fructose and 1.7% of glucose.
(2) Carrying out decoloration, ion exchange and evaporation concentration on the isomaltulose liquid obtained in the step (1) to obtain isomaltulose purified liquid;
the isomaltulose purifying liquid comprises the following components: 73.2% of isomaltulose, 17.4% of trehalulose, 3.8% of fructose and 1.7% of glucose.
(3) Hydrogenation reduction of isomaltulose to isomalt: adjusting the pH of the isomaltulose purified liquid obtained in the step (2) to 8.5 for catalytic hydrogenation to obtain isomaltulose liquid;
the catalytic hydrogenation conditions are as follows: the concentration of the material is 40 percent, the catalyst is Raney nickel, the addition amount is 15 percent of the dry basis of the material, the temperature is 125 ℃, the pressure is 10Mpa, and the reaction is stopped when the conversion rate reaches more than 98 percent;
the isomaltulose alcohol solution comprises the following components: 85.7% of isomalt, 9.2% of sorbitol and 1.8% of mannitol;
(4) isomalt chromatography: carrying out ion exchange on the isomaltitol solution obtained in the step (3) to remove ions, and then carrying out simulated moving bed chromatographic purification to obtain an isomaltitol component and a fusel component;
the simulated moving bed chromatographic purification conditions are as follows: eluent is water, separating agent is calcium type strong acid cation exchange resin, the content of solid matters in the feed is 58%, and the separation temperature is 80 ℃;
the isomaltulose alcohol content in the obtained isomaltulose alcohol component was 98.6%, the sorbitol content in the fusel component was 52.1%, the isomaltulose alcohol content was 29.6%, and the mannitol content was 17%.
(5) And (3) preliminarily concentrating the isomaltulose alcohol component obtained in the step (4) by adopting an evaporator, continuously concentrating by adopting a vacuum scraper evaporator until the solid content is more than 90 percent to obtain molten isomaltulose alcohol, and preserving the heat of the molten isomaltulose alcohol at 150 ℃.
(6) Isomalt crystallization: spraying the heat-preserved molten isomaltitol obtained in the step (5) onto the suspended isomaltitol seed crystal through a nozzle, and stirring while spraying to crystallize the isomaltitol;
the spraying is realized by hot compressed air, the temperature of the hot air is 150 ℃, and the pressure is 0.6 Mpa;
the isomalt seed in suspension is achieved by: putting isomaltitol seed crystals into a crystallizer in advance, stirring by using a double-helix stirrer, lifting the materials upwards by the self-transmission of the helix, and making the materials move horizontally by revolution, thereby achieving the purpose of suspending the materials;
the mass ratio of the molten isomalt to the suspended isomalt seed crystal was 2: 1.
(7) And (4) cooling, drying, crushing and screening the crystallized isomaltitol obtained in the step (6) to obtain an isomaltitol product.
(8) And (4) carrying out ion exchange and evaporation concentration on the fusel component obtained in the step (4) to obtain a liquid sugar alcohol product.
Example three:
this example is a process for the preparation of isomalt comprising the steps of:
(1) sucrose conversion to isomaltulose: preparing sucrose into a sucrose solution, adjusting the concentration of the sucrose solution to be 20 percent, adjusting the pH value to be 5.0, adding the sucrose solution into an immobilized bacterium with sucrose isomerase, wherein the weight of the immobilized bacterium is 8 percent of the weight of the sucrose solution, the temperature is 31 ℃, introducing sterile compressed air, and converting sucrose into isomaltulose under the condition of pressure of 0.06Mpa to obtain isomaltulose liquid;
the isomaltulose solution had the following composition: 78.5% of isomaltulose, 16.7% of trehalulose, 2.9% of fructose and 1.1% of glucose.
(2) Carrying out decoloration, ion exchange and evaporation concentration on the isomaltulose liquid obtained in the step (1) to obtain isomaltulose purified liquid;
the isomaltulose purifying liquid comprises the following components: 78.5% of isomaltulose, 16.7% of trehalulose, 2.9% of fructose and 1.1% of glucose.
(3) Hydrogenation reduction of isomaltulose to isomalt: adjusting the pH value of the isomaltulose purified liquid obtained in the step (2) to 6.5 for catalytic hydrogenation to obtain isomaltulose liquid;
the catalytic hydrogenation conditions are as follows: the concentration of the material is 40 percent, the catalyst is Raney nickel, the addition amount is 5 percent of the dry basis of the material, the temperature is 125 ℃, the pressure is 10Mpa, and the reaction is stopped when the conversion rate reaches more than 98 percent;
the isomaltulose alcohol solution comprises the following components: 91.3% of isomalt, 7.4% of sorbitol and 1.1% of mannitol;
(4) isomalt chromatography: carrying out ion exchange on the isomaltitol solution obtained in the step (3) to remove ions, and then carrying out simulated moving bed chromatographic purification to obtain an isomaltitol component and a fusel component;
the simulated moving bed chromatographic purification conditions are as follows: eluent is water, separating agent is calcium type strong acid cation exchange resin, the solid content of the feed is 56%, and the separation temperature is 80 ℃;
the isomaltulose alcohol component obtained contained 99.4% isomalt, the sorbitol component contained 56.3%, isomalt 32.5%, and mannitol 18.3%.
(5) And (3) primarily concentrating the isomaltulose alcohol component obtained in the step (4) by adopting an evaporator, continuously concentrating by adopting a vacuum scraper evaporator until the solid content is more than 90 percent to obtain molten isomaltulose alcohol, and preserving the heat of the molten isomaltulose alcohol at the temperature of 130 ℃.
(6) Isomalt crystallization: spraying the heat-preserved molten isomaltitol obtained in the step (5) onto the suspended isomaltitol seed crystal through a nozzle, and stirring while spraying to crystallize the isomaltitol;
the spraying is realized by hot compressed air, the temperature of the hot air is 150 ℃, and the pressure is 0.7 Mpa;
the isomalt seed in suspension is achieved by: putting isomaltitol seed crystals into a crystallizer in advance, stirring by using a double-helix stirrer, lifting the materials upwards by the self-transmission of the helix, and making the materials move horizontally by revolution, thereby achieving the purpose of suspending the materials;
the mass ratio of the molten isomalt to the suspended isomalt seed crystal was 1: 2.
(7) And (4) cooling, drying, crushing and screening the crystallized isomaltitol obtained in the step (6) to obtain an isomaltitol product.
(8) And (4) carrying out ion exchange and evaporation concentration on the fusel component obtained in the step (4) to obtain a liquid sugar alcohol product.
The technical features of the present invention which are not described in the above embodiments may be implemented by or using the prior art, and are not described herein again, of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and variations, modifications, additions or substitutions which may be made by those skilled in the art within the spirit and scope of the present invention should also fall within the protection scope of the present invention.

Claims (8)

1. A method for preparing isomalt, which is characterized by comprising the following steps:
(1) sucrose conversion to isomaltulose: preparing sucrose into a sucrose solution with a certain concentration, adding the sucrose solution into immobilized bacteria with sucrose isomerase, and converting sucrose into isomaltulose under a certain condition to obtain isomaltulose liquid;
(2) carrying out decoloration, ion exchange and evaporation concentration on the isomaltulose liquid obtained in the step (1) to obtain isomaltulose purified liquid;
(3) hydrogenation reduction of isomaltulose to isomalt: adjusting the pH of the isomaltulose purified liquid obtained in the step (2) to 6.5-8.5, and carrying out catalytic hydrogenation to obtain isomaltulose liquid;
(4) isomalt chromatography: carrying out ion exchange on the isomaltitol solution obtained in the step (3) to remove ions, and then carrying out simulated moving bed chromatographic purification to obtain an isomaltitol component and a fusel component;
(5) preliminarily concentrating the isomaltulose alcohol component obtained in the step (4) by adopting an evaporator, continuously concentrating by adopting a vacuum scraper evaporator until the solid content is more than 90%, obtaining molten isomaltulose alcohol, and preserving heat of the molten isomaltulose alcohol;
(6) isomalt crystallization: spraying the heat-preserved molten isomaltitol obtained in the step (5) onto the suspended isomaltitol seed crystal through a nozzle, and stirring while spraying to crystallize the isomaltitol;
(7) cooling, drying, crushing and screening the crystallized isomaltitol obtained in the step (6) to obtain an isomaltitol product;
(8) and (4) carrying out ion exchange and evaporation concentration on the fusel component obtained in the step (4) to obtain a liquid sugar alcohol product.
2. The method according to claim 1, wherein the concentration of the sucrose solution in the step (1) is 20-55%, and the weight of the immobilized cells is 8-12% of the weight of the sucrose solution.
3. The process according to claim 1, wherein the conditions in step (1) are: pH5.0-8.0, temperature is 25-32 deg.C, and sterile compressed air is introduced under pressure of 0.03-0.06 MPa.
4. The process for producing isomalt according to claim 1, wherein the isomalt solution obtained in step (1) has the following composition: 73-80% of isomaltulose, 13-18% of trehalulose, 2-4% of fructose and 0.5-2% of glucose.
5. The process according to claim 1, wherein the catalytic hydrogenation conditions in step (3) are as follows: the concentration of the material is 30-50%, the catalyst is Raney nickel, the addition amount of the Raney nickel is 5-15% of the dry basis of the material, the temperature is 100-130 ℃, and the pressure is 5-13 Mpa;
the isomaltulose alcohol solution in the step (3) comprises the following components: 84-92% of isomalt, 6-10% of sorbitol and 1-2% of mannitol.
6. The process for preparing isomalt according to claim 1, wherein the simulated moving bed chromatographic purification conditions in step (4) are as follows: eluent is water, separating agent is calcium type strong acid cation exchange resin, the content of solid matters in the feed is 30-60%, and the separation temperature is 50-80 ℃;
in the step (4), the isomaltitol component contains isomaltitol more than 97%, and the fusel component contains sorbitol 50-57%, isomaltitol 28-35%, and mannitol 15-22%.
7. The process according to claim 1, wherein the temperature in the step (5) is 125-150 ℃.
8. The process according to claim 1, wherein the spraying in step (6) is carried out by hot compressed air at a temperature of 125 ℃ and 150 ℃ and a pressure of 0.4 to 0.7 MPa;
the mass ratio of the molten isomaltitol to the suspended isomaltitol seed crystal is (1-2): (1-2).
CN202110129658.2A 2021-01-29 2021-01-29 Preparation method of isomalt Pending CN112920235A (en)

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Publication number Priority date Publication date Assignee Title
CN112888697A (en) * 2018-09-11 2021-06-01 祖德楚克尔股公司 Improved method for producing sweetener
CN116987749A (en) * 2023-09-27 2023-11-03 广州医科大学附属第一医院（广州呼吸中心） Method for producing isomaltulose alcohol by catalyzing sucrose through multienzyme cascade reaction and application of method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000064916A1 (en) * 1999-04-23 2000-11-02 Xyrofin Oy Crystallization of glucopyranosylalditols, crystalline glucopyranosylalditol product and use thereof
US20060147597A1 (en) * 2004-12-21 2006-07-06 Pierrick Duflot Method for making a powder containing crystalline particles of glucopyranosyl-alditols
CN102964399A (en) * 2012-11-23 2013-03-13 浙江华康药业股份有限公司 Method for preparing isomalt powder
CN104894191A (en) * 2015-05-29 2015-09-09 湖南尔康制药股份有限公司 Preparation method of isomaltitol
US20160160251A1 (en) * 2013-07-18 2016-06-09 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Optimized Method For Producing A Composition Containing Isomaltulose
CN105969824A (en) * 2016-07-25 2016-09-28 山东百龙创园生物科技有限公司 Preparation method of palatinitol
CN110746469A (en) * 2019-11-07 2020-02-04 青岛科海生物有限公司 Method for separating isomaltulose mother liquor by simulated moving bed
WO2020053207A1 (en) * 2018-09-11 2020-03-19 Südzucker AG Method for improved production of isomalt

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000064916A1 (en) * 1999-04-23 2000-11-02 Xyrofin Oy Crystallization of glucopyranosylalditols, crystalline glucopyranosylalditol product and use thereof
US20060147597A1 (en) * 2004-12-21 2006-07-06 Pierrick Duflot Method for making a powder containing crystalline particles of glucopyranosyl-alditols
CN102964399A (en) * 2012-11-23 2013-03-13 浙江华康药业股份有限公司 Method for preparing isomalt powder
US20160160251A1 (en) * 2013-07-18 2016-06-09 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Optimized Method For Producing A Composition Containing Isomaltulose
CN104894191A (en) * 2015-05-29 2015-09-09 湖南尔康制药股份有限公司 Preparation method of isomaltitol
CN105969824A (en) * 2016-07-25 2016-09-28 山东百龙创园生物科技有限公司 Preparation method of palatinitol
WO2020053207A1 (en) * 2018-09-11 2020-03-19 Südzucker AG Method for improved production of isomalt
CN110746469A (en) * 2019-11-07 2020-02-04 青岛科海生物有限公司 Method for separating isomaltulose mother liquor by simulated moving bed

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112888697A (en) * 2018-09-11 2021-06-01 祖德楚克尔股公司 Improved method for producing sweetener
CN116987749A (en) * 2023-09-27 2023-11-03 广州医科大学附属第一医院（广州呼吸中心） Method for producing isomaltulose alcohol by catalyzing sucrose through multienzyme cascade reaction and application of method
CN116987749B (en) * 2023-09-27 2023-12-01 广州医科大学附属第一医院（广州呼吸中心） Method for producing isomaltulose alcohol by catalyzing sucrose through multienzyme cascade reaction and application of method

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