CN112916062A - Driving method for liquid flow of microfluidic chip - Google Patents
Driving method for liquid flow of microfluidic chip Download PDFInfo
- Publication number
- CN112916062A CN112916062A CN202110130473.3A CN202110130473A CN112916062A CN 112916062 A CN112916062 A CN 112916062A CN 202110130473 A CN202110130473 A CN 202110130473A CN 112916062 A CN112916062 A CN 112916062A
- Authority
- CN
- China
- Prior art keywords
- branch
- main
- liquid
- flow channel
- isolation film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
Abstract
The invention relates to a method for driving liquid flow in a microfluidic chip, which is characterized by comprising the following steps: in the process that liquid dispersedly flows to branches in a main flow channel of the main chip, the branch well is communicated with the negative pressure flow channel through the isolation film, the negative pressure flow channel is communicated with all or part of the branch wells through the isolation film according to needs, different negative pressures are applied to the negative pressure flow channel according to needs or thrust is generated in the main flow channel, and therefore the speed of the main flow channel entering the flow channel branches and the branch wells is controlled.
Description
Technical Field
The invention relates to a driving method for liquid flow of a microfluidic chip.
Background
The driving mode of liquid flowing in the microfluidic chip is mainly thrust or suction; in the flow channel of the micro-fluidic chip, only the main flow channel is arranged, or the main flow channel is less, the main flow channel is provided with an outlet, the manufacture and the use are not influenced, but when branch flow channels exist, when branches do not collect and flow out to the main flow channel, liquid is difficult to flow to the branch flow channels and fill branch wells at the tail ends of the branch flow channels, the branch flow channels and the reaction wells of the branch flow channels are willing to contain gas, and the gas can enter only when being discharged;
however, if the outlets are arranged as the outlets of the main flow path, in order to prevent the branch flow paths from interfering with each other, it is necessary to arrange a large number of branch flow path outlets, which is obviously not practical.
At present, because PDMS has certain air permeability, a plurality of chips use PDMS as a main body of a microfluidic chip to avoid the problems, however, because PDMS is only suitable for laboratory manufacture at present and is difficult to produce in large quantities, a new technical scheme is provided to realize the driving of liquid in the branches of the microfluidic chip.
Disclosure of Invention
The invention provides a driving method for liquid flowing of a micro-fluidic chip, which helps liquid to fully enter a branch flow channel and a branch well of the branch flow channel in the process that the liquid in the micro-fluidic chip flows from a main flow channel to the branch flow channel, ensures that each branch well is independent from each other, and can control the speed of entering the branch well. The method can realize batch and industrial production, thereby realizing rapid production.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for driving liquid in a microfluidic chip is characterized in that: in the process that liquid flows in a distributed mode from a main channel of a main chip to a channel branch, the positions of branch wells are communicated with negative pressure channels through isolation films, the negative pressure channels are communicated with all or part of the branch wells through the isolation films as required, different negative pressures are applied to the negative pressure channels as required or thrust is generated in the main channel, and therefore the speed of the main channel entering the channel branch channels and the branch wells is controlled.
The liquid driving method in the microfluidic chip comprises the following steps: the isolating membrane is a whole air-permeable and water-impermeable membrane directly covering the microfluidic main chip and is used as an upper cover of a runner groove of the microfluidic chip.
The liquid driving method in the microfluidic chip comprises the following steps: in order to control the speed of flowing into the branch well, the thickness and the air permeability of the isolation film are adjustable, so that the speed of the liquid in the main flow channel entering the flow channel branch and the branch well is better controlled in cooperation with the application of negative pressure.
The liquid driving method in the microfluidic chip comprises the following steps: because of the permeability of the isolation film, the thickness of the isolation film is limited, so in order to protect the isolation film from being damaged in use, an upper plate is added on the corresponding surface of the isolation film, the upper plate is used for protecting the isolation film, under the condition of not applying negative pressure, the main chip and the isolation film can be used and realize some functions by using the main chip and the isolation film independently without the upper plate, and the liquid can also enter the flow channel branch and the branch well;
the negative pressure flow channel is additionally arranged on the upper plate, is communicated with the branch well on the main chip, and is divided by the isolating membrane in the middle.
The liquid driving method in the microfluidic chip comprises the following steps: when the driving mode used by the liquid in the main flow channel in the main chip is a propelling mode, when the outlet of the main flow channel is closed or the size of the outlet is changed, the speed of the main flow channel entering the flow channel branch can be changed due to the fact that the liquid in the main flow channel receives the thrust.
The liquid driving method in the microfluidic chip comprises the following steps: the isolating membrane is breathable and liquid-tight, the branch wells are mutually independent in the main chip, and the liquid or reaction in the branch wells cannot interfere with each other to influence the result.
The liquid driving method in the microfluidic chip comprises the following steps: the branch wells on the main chip are independent of each other.
The method for driving the liquid in the microfluidic chip mainly realizes the method for the main runner liquid in the microfluidic chip to enter the branch well. The advantage of this method is that
1. Liquid can fully enter the branch well;
2. the liquids in the branch wells are independent from each other and do not influence each other;
3. the whole isolation film is used, so that rapid mass production can be realized, and the manufacturing difficulty is greatly reduced;
4. the thickness and the air permeability of the isolating membrane are used for controlling the speed of liquid entering the branch well, so that the reaction process flow in the branch well can be well controlled.
Therefore, the method for driving the liquid by communicating the branch well with negative pressure or atmosphere by using the isolation film on the microfluidic chip is a technical scheme suitable for mass production.
Drawings
FIG. 1 is a schematic diagram of a microfluidic chip.
Fig. 2 is a structure diagram of a main chip of the microfluidic core.
FIG. 3 is a top plate structure of the microfluidic chip.
FIG. 4 is a view showing the structure of a separation membrane of a microfluidic chip.
Description of reference numerals: 1-a main chip; 11-a liquid inlet; 12-a main flow channel; 13-flow channel branching; 14-a lateral well; 15-outlet at the tail end of the main runner, and 2, arranging an upper plate; 21-a negative pressure flow channel; 22-a negative pressure outlet and 23-a liquid inlet avoidance port; 24-a main runner tail end outlet avoidance port; 3-isolating film, 31-liquid inlet avoiding port.
Detailed Description
As shown in fig. 1 to 4, in one embodiment of the microfluidic chip for detection, a liquid flows from a liquid inlet 11 to an outlet 15 at the end of a main channel through the main channel 12, and in order to fill the channel branches 13 and the branch wells 14 with the liquid, after the main channel 12 and the channel branches 13 of the main chip 1 are opened and covered by the isolation film 3, the whole main chip 1 is a closed chip, except for the avoidance port, since the isolation film 3 is gas-permeable and water-impermeable, since the isolation film 3 is located above the main channel 12, the channel branch 13 and the branch well 14, the liquid is also sealed on the main chip 1, the negative pressure flow passage 21 of the upper plate 2 communicates with the area of the isolation diaphragm 3 above each of the branch wells 14, so that the inspection liquid in the main flow channel 12 fills the branch well 14 by self-diffusion or negative pressure after the liquid enters the flow channel branch 13.
The embodiment is an implementation case of the method, and any air-permeable and liquid-tight isolating membrane which is used for pushing the liquid inlet of the branch well and the branch well by negative pressure or positive pressure falls into the protection scope of the invention.
Claims (7)
1. A method for driving liquid in a microfluidic chip is characterized in that: in the process that liquid flows in a distributed mode from a main channel of a main chip to a channel branch, the positions of branch wells are communicated with negative pressure channels through isolation films, the negative pressure channels are communicated with all or part of the branch wells through the isolation films as required, different negative pressures are applied to the negative pressure channels as required or thrust is generated in the main channel, and therefore the speed of the main channel entering the channel branch channels and the branch wells is controlled.
2. The method for driving liquid in a microfluidic chip according to claim 1, wherein: the isolating membrane is a whole air-permeable and water-impermeable membrane directly covering the microfluidic main chip and is used as an upper cover of a runner groove of the microfluidic chip.
3. The method for driving liquid in a microfluidic chip according to claim 1, wherein: in order to control the speed of flowing into the branch well, the thickness and the air permeability of the isolation film are adjustable, so that the speed of the liquid in the main flow channel entering the flow channel branch and the branch well is better controlled in cooperation with the application of negative pressure.
4. The method for driving liquid in a microfluidic chip according to claim 1, wherein: because of the permeability of the isolation film, the thickness of the isolation film is limited, so in order to protect the isolation film from being damaged in use, an upper plate is added on the corresponding surface of the isolation film, the upper plate is used for protecting the isolation film, under the condition of not applying negative pressure, the main chip and the isolation film can be used and realize some functions by using the main chip and the isolation film independently without the upper plate, and the liquid can also enter the flow channel branch and the branch well;
the negative pressure flow channel is additionally arranged on the upper plate, is communicated with the branch well on the main chip, and is divided by the isolating membrane in the middle.
5. The method for driving liquid in a microfluidic chip according to claim 1, wherein: when the driving mode used by the liquid in the main flow channel in the main chip is a propelling mode, when the outlet of the main flow channel is closed or the size of the outlet is changed, the speed of the main flow channel entering the flow channel branch can be changed due to the fact that the liquid in the main flow channel receives the thrust.
6. The method for driving liquid in a microfluidic chip according to claim 1, wherein: the isolating membrane is breathable and liquid-tight, the branch wells are mutually independent in the main chip, and the liquid or reaction in the branch wells cannot interfere with each other to influence the result.
7. The method for driving liquid in a microfluidic chip according to claim 1, wherein: the branch wells on the main chip are independent of each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110130473.3A CN112916062A (en) | 2021-01-29 | 2021-01-29 | Driving method for liquid flow of microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110130473.3A CN112916062A (en) | 2021-01-29 | 2021-01-29 | Driving method for liquid flow of microfluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112916062A true CN112916062A (en) | 2021-06-08 |
Family
ID=76168846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110130473.3A Pending CN112916062A (en) | 2021-01-29 | 2021-01-29 | Driving method for liquid flow of microfluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112916062A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113522387A (en) * | 2021-07-23 | 2021-10-22 | 江苏溢康辰医疗科技有限公司 | Bidirectional active micro-fluidic chip and application method thereof |
| CN114082456A (en) * | 2021-10-29 | 2022-02-25 | 重庆大学 | Micro-fluid driving system based on porous membrane regulation and control, driving mode and application thereof |
| CN114849799A (en) * | 2022-03-28 | 2022-08-05 | 哈尔滨医科大学 | Micro-fluidic detection chip |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897978A (en) * | 2012-12-26 | 2014-07-02 | 中国科学院苏州纳米技术与纳米仿生研究所 | Microfluidic microbe culture chip |
| CN108339580A (en) * | 2018-03-20 | 2018-07-31 | 哈尔滨工业大学深圳研究生院 | A kind of fluid shearing force generating mechanism and hydrodynamic shear generation method |
| CN208449339U (en) * | 2018-05-04 | 2019-02-01 | 南京邮电大学 | A kind of MHD micro-fluidic chip of star-tree-shaped composite construction |
| CN111282605A (en) * | 2020-02-17 | 2020-06-16 | 苏州大学 | Microfluidic chip and application method thereof |
-
2021
- 2021-01-29 CN CN202110130473.3A patent/CN112916062A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897978A (en) * | 2012-12-26 | 2014-07-02 | 中国科学院苏州纳米技术与纳米仿生研究所 | Microfluidic microbe culture chip |
| CN108339580A (en) * | 2018-03-20 | 2018-07-31 | 哈尔滨工业大学深圳研究生院 | A kind of fluid shearing force generating mechanism and hydrodynamic shear generation method |
| CN208449339U (en) * | 2018-05-04 | 2019-02-01 | 南京邮电大学 | A kind of MHD micro-fluidic chip of star-tree-shaped composite construction |
| CN111282605A (en) * | 2020-02-17 | 2020-06-16 | 苏州大学 | Microfluidic chip and application method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113522387A (en) * | 2021-07-23 | 2021-10-22 | 江苏溢康辰医疗科技有限公司 | Bidirectional active micro-fluidic chip and application method thereof |
| CN114082456A (en) * | 2021-10-29 | 2022-02-25 | 重庆大学 | Micro-fluid driving system based on porous membrane regulation and control, driving mode and application thereof |
| CN114849799A (en) * | 2022-03-28 | 2022-08-05 | 哈尔滨医科大学 | Micro-fluidic detection chip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112916062A (en) | Driving method for liquid flow of microfluidic chip | |
| CN107739706B (en) | Multi-flux microfluidic nucleic acid detection chip for actively controlling flow path and application method thereof | |
| CN112566722B (en) | Microfluidic device and method for separating, purifying and concentrating components of a fluid medium | |
| US7794611B2 (en) | Micropump for integrated device for biological analyses | |
| JP4368914B2 (en) | Manifold adapter plate for filtration equipment | |
| KR101922627B1 (en) | Multiplexor for control of flow in microfluidics chip and microfluidics chip assembly | |
| CA2618164C (en) | Cartridge having variable volume reservoirs | |
| KR100773563B1 (en) | Micro fluidics device with micro fluid treating element and method for fabricating the same | |
| ITBO20090155A1 (en) | METHOD FOR INSULATING PARTICLES | |
| CN102427883A (en) | Microfluidic device for isolation of cells | |
| CA3072484C (en) | An injection molded microfluidic/fluidic cartridge integrated with silicon-based sensor | |
| KR101252829B1 (en) | Array apparatus for separation of single cell | |
| CN107557452A (en) | A kind of micro-fluidic chip for nucleic acid amplification detection | |
| ITBO20090154A1 (en) | MICROFLUID SYSTEM | |
| CN113234583A (en) | Cell reaction module integrated with gas circuit control and method for carrying out cell reaction by adopting cell reaction module | |
| CN207498376U (en) | The micro-fluidic nucleic acid detection chip of more flux of active control flow path | |
| CN113522383A (en) | Cell working condition device | |
| CN106195439A (en) | Micro-valve system based on stream state | |
| CN1331575C (en) | Implementation of microfluidic components in a microfluidic system | |
| CN115041244B (en) | Microfluidic chip for generating and wrapping microorganism liquid drops in multiple channels | |
| SG176172A1 (en) | A microfluidic device | |
| Skelley et al. | An active, integrated bubble trap and debubbler for microfluidic applications | |
| CN116034153A (en) | High flux detection chip | |
| ITBO20100503A1 (en) | MICROFLUID SYSTEM | |
| ITBO20100502A1 (en) | MICROFLUID SYSTEM |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |