CN112915634B - Bacillus subtilis fermentation liquid concentration device - Google Patents
Bacillus subtilis fermentation liquid concentration device Download PDFInfo
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- CN112915634B CN112915634B CN202110111085.0A CN202110111085A CN112915634B CN 112915634 B CN112915634 B CN 112915634B CN 202110111085 A CN202110111085 A CN 202110111085A CN 112915634 B CN112915634 B CN 112915634B
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- bacillus subtilis
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- 239000007788 liquid Substances 0.000 title claims abstract description 73
- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 55
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 55
- 238000000855 fermentation Methods 0.000 title claims abstract description 43
- 230000004151 fermentation Effects 0.000 title claims abstract description 43
- 238000001914 filtration Methods 0.000 claims abstract description 79
- 239000012528 membrane Substances 0.000 claims abstract description 15
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 239000007787 solid Substances 0.000 claims description 18
- 230000002093 peripheral effect Effects 0.000 claims description 14
- 230000007246 mechanism Effects 0.000 claims description 12
- 239000002699 waste material Substances 0.000 claims description 12
- 238000009434 installation Methods 0.000 claims description 9
- 210000005056 cell body Anatomy 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000007790 scraping Methods 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 239000012535 impurity Substances 0.000 description 15
- 230000009471 action Effects 0.000 description 8
- 239000012634 fragment Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000002213 purine nucleotide Substances 0.000 description 2
- 125000000561 purinyl group Chemical class N1=C(N=C2N=CNC2=C1)* 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
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- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/11—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
- B01D29/31—Self-supporting filtering elements
- B01D29/35—Self-supporting filtering elements arranged for outward flow filtration
- B01D29/353—Self-supporting filtering elements arranged for outward flow filtration with corrugated, folded filtering elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/96—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor in which the filtering elements are moved between filtering operations; Particular measures for removing or replacing the filtering elements; Transport systems for filters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/16—Feed pretreatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
- B01D63/082—Flat membrane modules comprising a stack of flat membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
- C12M1/126—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means with hollow fibres or tubular filter elements
Abstract
The invention discloses a bacillus subtilis fermentation liquor concentration device, which belongs to the field of biological concentration equipment, wherein the concentration bin further comprises a first support plate and a second support plate which are obliquely arranged and can guide liquid, a channel which is arranged on each support plate and is of a corrugated structure, and a flow guide groove body which is fixedly connected to each support plate through bolts and covers the channel on the flow guide groove body, the section of the flow guide groove body is of a semi-ring structure, a flow channel which can be penetrated by the liquid and is of a corrugated structure is formed between the flow guide groove body and the support plates, and an ultrafiltration membrane which covers the channel on the flow guide groove body and is pressed on the periphery of the flow guide groove body is laid on each support plate. According to the invention, through the arrangement of conducting circuitous diversion on the liquid containing the bacillus subtilis and conducting multi-stage concentration and filtration on the liquid, the flowing time of the bacillus subtilis fermentation liquor in the concentration bin is prolonged, and the concentration effect of the concentration device on the bacillus subtilis fermentation liquor is effectively improved.
Description
Technical Field
The invention relates to the field of biological concentration equipment, in particular to a bacillus subtilis fermentation liquor concentration device.
Background
Bacillus subtilis is a species of Bacillus. Without capsule, the perigenic flagellum can move. The thallus is not expanded after sporulation. The colony surface is rough and opaque, and is dirty white or yellowish, and when the colony grows in a liquid culture medium, the skin becomes always formed. Aerobic bacteria. Tryptophan can be decomposed to form indole by using protein, various sugars and starch. The application in genetic research is wide, and the research on the synthetic pathway of purine nucleotide and the regulation mechanism of the purine nucleotide in the bacterium is clear. Widely distributed in soil and putrefactive organic matters, and is easy to propagate in the Sucus subtilis extract.
The existing device for concentrating the bacillus subtilis fermentation liquor cannot perform multi-stage concentration treatment on the fermentation liquor and cannot prolong the flowing time of the fermentation liquor in the device, so that the concentration effect of the device on the fermentation liquor is influenced, and therefore, the development of the bacillus subtilis fermentation liquor concentration device is urgently needed.
Disclosure of Invention
1. Technical problem to be solved
The invention aims to provide a bacillus subtilis fermentation liquor concentration device to solve the problems in the background technology.
2. Technical scheme
In order to solve the problems, the invention adopts the following technical scheme:
the utility model provides a bacillus subtilis zymotic fluid enrichment facility, includes filtering mechanism and concentration mechanism, filtering mechanism includes filters the storehouse and locates the inside filtration structure of crossing the storehouse, it is equipped with the inlet on the storehouse to cross the filter, concentration mechanism includes concentration bin, be equipped with the milipore filter that filters bacillus subtilis on the concentration bin, and still be equipped with liquid outlet, waste discharge port on the concentration bin, still install the connecting pipe one that is used for communicateing concentration bin on the filtration bin, concentration bin still includes two slope settings and can carry out the backup pad one, the backup pad two of water conservancy diversion to liquid, set up on every backup pad and be the passageway of ripple texture, through the water conservancy diversion cell body that bolt fixed connection just covers its passageway in every backup pad, the cross-section of water conservancy diversion cell body is half ring body structure, and forms the runner that can supply liquid to pass and be the waveform structure between water conservancy diversion cell body and the backup pad, an ultrafiltration membrane which covers the upper channel of each support plate and is pressed on the periphery of the guide groove body is laid on each support plate;
the inclination directions of the first support plate and the second support plate are opposite, the bottom end of the first connecting pipe is inserted into the highest end of the flow guide groove body on the first support plate, the bottom end of the first support plate is provided with a second connecting pipe which guides the liquid at the lowest end of the flow guide groove body on the first support plate to the second support plate, the lower end of the first support plate is provided with a communicating port communicated with the second connecting pipe, and the bottom end of each support plate is provided with a flow guide plate which guides the liquid passing through the corresponding ultrafiltration membrane;
the liquid outlet is provided with two, and is equipped with liquid outlet one between the guide plate on backup pad one and its bottom to be equipped with liquid outlet two between the guide plate on backup pad two and its bottom, liquid outlet one, liquid outlet two all are located the below of corresponding backup pad low-end department, offer on the lateral wall in concentrated storehouse and be used for the waste liquid exhaust's in the backup pad two waste discharge mouth.
Preferably, filtration is including being the fretwork sleeve that cup joints the connection in proper order, every adjacent two the fretwork sleeve all passes through sliding construction swing joint, and is equipped with on the telescopic peripheral border of fretwork of diameter the biggest and filters the connecting block that the storehouse inlet port is connected, fretwork sleeve, filtration storehouse, connecting block all set up to rectangle column structure, and filter and offer the installation breach that is used for installing the connecting block on the inlet port in storehouse to it has sealing washer three, every to glue on the terminal surface of connecting block and installation breach contact equally interval on the telescopic peripheral border of fretwork offers and carries out filterable through-hole to the solution that contains bacillus subtilis, and the minimum solid sleeve of diameter has solid sleeve through another sliding construction swing joint, the inlet in filtration storehouse is served and has the apron, and the inlet is located on the apron.
Preferably, the sliding structure includes that the equidistant bar spout of seting up on every sleeve periphery border, slides and locates every bar spout inboard and the other end all with corresponding side sleeve inner wall fixed connection's slider, every the inboard of bar spout all is equipped with the gag lever post rather than being parallel, and every gag lever post all runs through corresponding slider and with slider sliding connection, every all be equipped with the gyro wheel with the contact of bar spout on the slider.
Preferably, every all cup joint the spring that carries out elastic support to corresponding side slider on the gag lever post, all sleeves all are located the telescopic inboard that the diameter is the biggest when the spring is in natural state, all are equipped with sealing washer four between every two adjacent sleeves, and every sealing washer four all sets up the peripheral week of corresponding sleeve left end and circles to the peripheral week of every sealing washer four all is used for scribbling the piece of scraping on the corresponding sleeve inner wall, installation breach bottom is equipped with scribbles the water conservancy diversion notch one that scrapes the piece that gets off and accept on the sleeve inner wall, and filters the collecting vat that the bottom in storehouse was pegged graft and is had the collecting vat that communicates with water conservancy diversion notch one to the collecting vat passes through the screw and is connected with filtering the storehouse.
Preferably, the right-hand member lateral wall through type that filters the storehouse is equipped with the push rod, and the push rod is located the inboard one end of filter storehouse and is equipped with the support that is cross structure to the length of support slightly is less than the telescopic diameter of fretwork of the biggest diameter, the sleeve can take place to slide under the promotion of support, set up the perforation that supplies the push rod to pass on the right-hand member lateral wall of filter storehouse, and it has sealing washer one to glue on the fenestrate inner wall, the one end that the push rod is located filter storehouse is equipped with the push pedal.
Preferably, each guide groove body is provided with an inserting hole for inserting the bottom end of the corresponding connecting pipe at the highest end, and each inserting hole is adhered with a second sealing ring for sealing between the connecting pipe and the guide groove body.
Preferably, the second connecting pipe penetrates through the guide plate, another inserting hole for the bottom end of the first connecting pipe to vertically penetrate is formed in the guide plate below the first supporting plate, another sealing ring II is arranged on the inserting hole, the cross section of the guide plate is of an annular structure, each guide plate is parallel to the corresponding supporting plate, and the two ends of each guide plate are fixedly connected with the bottom end of the corresponding supporting plate.
Preferably, set up the water conservancy diversion notch two with a connecting pipe top intercommunication on filtering the inner wall in storehouse, filter storehouse, filtration, push rod all incline and mutual parallel arrangement, and water conservancy diversion notch two locates the low end department of filtering the storehouse to the high end department of filtering the storehouse is located to the inlet, the support is parallel with telescopic annular surface.
Preferably, a sealing gasket is adhered to the end face, in contact with the corresponding ultrafiltration membrane, of each flow guide groove body.
3. Advantageous effects
1. The liquid passing through the filter bin flows into the first support plate through the connecting pipe, and flows in a circuitous manner on the first support plate along the flow guide groove body, the bacillus subtilis is filtered by the ultrafiltration membrane on the first support plate when the liquid flows on the flow guide groove body and falls onto the flow guide plate at the bottom end of the first support plate through the channel on the first support plate, the filtered bacillus subtilis solution can be guided and conveyed by the flow guide plate and discharged from the first liquid outlet, and the liquid flowing to the lower end of the first support plate can flow into the second support plate through the connecting pipe again, so that the circuitous flow guide groove body on the second support plate can guide the liquid again, the filtration of the bacillus subtilis in the liquid is realized in the guiding flow process, and the secondarily filtered bacillus subtilis solution can pass through the second support plate and fall into the flow guide plate below the second support plate, and finally, the liquid is discharged from the liquid outlet II, and the arrangement for conducting circuitous diversion on the liquid containing the bacillus subtilis and conducting multi-stage concentration and filtration on the liquid prolongs the flowing time of the bacillus subtilis fermentation liquid in the concentration bin and effectively improves the concentration effect of the concentration device on the bacillus subtilis fermentation liquid.
2. When the bacillus subtilis fermentation liquor enters the filtering bin, weed fragments or other impurities in the fermentation liquor can be filtered through the hollow sleeves which are sequentially sleeved and connected in a sliding mode, and when more fermentation liquor flows into the inner sides of the hollow sleeves at one time, the fermentation liquor can impact the solid sleeves with larger force, so that the multistage hollow sleeves can slide under the dragging of the solid sleeves, the expansion of the multistage hollow sleeves is realized, the filtering area of the filtering structure on the fermentation liquor is increased, and the filtering efficiency of the filtering structure is improved.
3. The spring is compressed when the hollow sleeve slides under the action of impact force, the hollow sleeve can be reset to provide power through the elastic resetting capacity of the spring, namely, when the acting force of the hollow sleeve impacted by water flow is reduced, the hollow sleeve can be restored under the elastic action of the spring, and when the hollow sleeve is restored, the impurities attached to the inner wall of the hollow sleeve can be scraped by the close fit of the four sealing rings to the inner side wall of the hollow sleeve at the corresponding side, therefore, the self-cleaning of the hollow sleeve is realized, the hollow sleeve still has better filtering performance when used next time, the impurities scraped by the sealing ring IV can fall into the inner wall of the first diversion notch, impurity that will filter out through water conservancy diversion notch one flows through the inside to the collecting vat, and just through the collecting vat and the screw connection between the filter bin, can make things convenient for the clearance of staff later stage to the inside impurity of collecting vat.
4. In order to avoid elastic fatigue of the spring or blockage of the hollow sleeve during filtering and incapability of resetting the spring under the impact action of water flow, the hollow sleeve and the solid sleeve can be pushed by manually pushing the push rod to push the support on the end part of the push rod, so that the sleeve is assisted to reset, and the hollow sleeve can be manually and quickly cleaned.
5. The guide plate is of an annular structure and is arranged in parallel with the corresponding support plate, so that the guide plate can guide the solution of the bacillus subtilis rod passing through the support plate in a two-way manner, the bacillus subtilis rod solution falling on the guide plate can be concentrated at the lower end of the arc of the guide plate and can flow along the length direction of the guide plate at the lower end of the arc of the guide plate, and finally flow to the lower end of the guide plate, and the gathering effect of the guide plate on the bacillus subtilis rod solution is improved.
6. The filter bin is obliquely arranged, so that the flowing speed of water flow at the inner side of the filter bin can be increased, the filtering efficiency of the filter bin on the bacillus subtilis fermentation liquor is improved, meanwhile, the gathering amount of the bacillus subtilis fermentation liquor at the water inlet port of the filter bin can be reduced, and the waste is reduced.
Drawings
FIG. 1 is a schematic front view of the internal structure of the present invention;
FIG. 2 is a schematic view of the connection of the support plate to the baffle;
FIG. 3 is a schematic side view of the filter cartridge;
FIG. 4 is a schematic view of the interior of the filter structure;
FIG. 5 is an enlarged schematic view of the structure at A in FIG. 1;
fig. 6 is an enlarged schematic view of a portion B in fig. 1.
Reference numerals: 1. a filtering bin; 2. a concentration bin; 3. a filter structure; 4. installing a notch; 5. a cover plate; 6. a liquid inlet; 7. a support; 8. a push rod; 9. perforating; 10. a first sealing ring; 11. pushing the plate; 12. a first flow guide notch; 13. collecting tank; 14. a first support plate; 15. a second support plate; 16. a diversion tank body; 17. ultrafiltration membranes; 18. a gasket; 19. a first connecting pipe; 20. a first liquid outlet; 21. a channel; 22. a baffle; 23. a communication port; 24. a liquid outlet II; 25. a waste discharge port; 26. a second sealing ring; 27. inserting holes; 28. a second diversion notch; 29. a second connecting pipe; 31. hollowing out the sleeve; 32. a solid sleeve; 33. a slider; 34. a strip-shaped chute; 35. a limiting rod; 36. a spring; 37. connecting blocks; 38. a third sealing ring; 39. a through hole; 310. a fourth sealing ring; 311. and a roller.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Examples
As shown in fig. 1-6, a bacillus subtilis fermentation liquid concentrating device comprises a filtering mechanism and a concentrating mechanism, the filtering mechanism comprises a filtering bin 1 and a filtering structure 3 arranged inside the filtering bin 1, a liquid inlet 6 is arranged on the filtering bin 1, the filtering structure 3 comprises hollow sleeves 31 which are sequentially connected in a sleeved manner, every two adjacent hollow sleeves 31 are movably connected through a sliding structure, a connecting block 37 connected with a water inlet of the filtering bin 1 is arranged on the peripheral circumference of the hollow sleeve 31 with the largest diameter, the hollow sleeves 31, the filtering bin 1 and the connecting block 37 are all of rectangular structures, an installation notch 4 for installing the connecting block 37 is arranged on the water inlet of the filtering bin 1, a sealing ring III 38 is adhered on the end face of the connecting block 37, which is in contact with the installation notch 4, through holes 39 for filtering a solution containing bacillus subtilis are arranged on the peripheral circumference of each hollow sleeve 31 at equal intervals, the solid sleeve 32 with the smallest diameter is movably connected with the solid sleeve 32 through another sliding structure, the water inlet end of the filter bin 1 is connected with the cover plate 5 through screws, the liquid inlet 6 is formed in the cover plate 5, when bacillus subtilis fermentation liquor enters the filter bin 1, weed fragments or other impurities in the fermentation liquor can be filtered through the hollow sleeves 31 which are sequentially sleeved and connected in a sliding mode, and when the fermentation liquor flowing into the inner side of the hollow sleeve 31 at one time is large, the fermentation liquor can impact the solid sleeve 32 with large force, so that the multistage hollow sleeve 31 can slide under the dragging of the solid sleeve 32, the multistage hollow sleeve 31 is unfolded, the filtering area of the filtering structure 3 on the fermentation liquor is increased, and the filtering efficiency of the filtering structure 3 is improved;
the sliding structure comprises strip-shaped sliding grooves 34 which are arranged on the peripheral circumference of each sleeve at equal intervals, sliding blocks 33 which are arranged on the inner sides of the strip-shaped sliding grooves 34 in a sliding mode, the other ends of the sliding blocks 33 are fixedly connected with the inner wall of the corresponding sleeve, limiting rods 35 which are parallel to the strip-shaped sliding grooves are arranged on the inner sides of the strip-shaped sliding grooves 34, each limiting rod 35 penetrates through the corresponding sliding block 33 and is in sliding connection with the corresponding sliding block 33, each sliding block 33 is provided with a roller 311 which is in contact with the strip-shaped sliding groove 34, when the multistage hollow sleeve 31 slides under the dragging of the solid sleeve 32, the sliding block 33 is in sliding connection with the limiting rods 35 to limit the sliding hollow sleeve 31, and therefore the stability of the hollow sleeve 31 in sliding is guaranteed;
each limiting rod 35 is sleeved with a spring 36 which elastically supports the corresponding side sliding block 33, all the sleeves are located on the inner side of the sleeve with the largest diameter when the spring 36 is in a natural state, a sealing ring four 310 is arranged between every two adjacent sleeves, each sealing ring four 310 is arranged on a peripheral ring at the left end of the corresponding sleeve, the peripheral ring of each sealing ring four 310 is used for scraping fragments on the inner wall of the corresponding sleeve, a flow guide notch one 12 which is used for receiving the fragments scraped from the inner wall of the sleeve is arranged at the bottom end of the installation notch 4, a collecting tank 13 which is communicated with the flow guide notch one 12 is inserted into the bottom end of the filtering bin 1, the collecting tank 13 is connected with the filtering bin 1 through screws, the spring 36 is compressed when the hollow sleeve 31 slides under the action of impact force, and power can be provided for the hollow sleeve 31 to reset through the elastic resetting capability of the spring 36, when the acting force of water flow impact on the hollowed-out sleeve 31 is reduced, the hollowed-out sleeve 31 can be restored under the elastic action of the spring 36, and when the hollowed-out sleeve 31 is restored, the inner side wall of the hollowed-out sleeve 31 on the corresponding side can be attached to the sealing ring IV 310 in a clinging mode, impurities attached to the inner wall of the hollowed-out sleeve 31 can be scraped, so that self cleaning of the hollowed-out sleeve 31 is achieved, the hollowed-out sleeve 31 can still have good filtering performance when used next time, the impurities scraped by the sealing ring IV 310 can fall onto the inner wall of the first flow guide notch 12, the filtered impurities are guided to the inside of the collecting tank 13 through the first flow guide notch 12, and the collecting tank 13 is connected with the filtering bin 1 through screws, so that a worker can clean the impurities in the collecting tank 13 in the later period conveniently;
a push rod 8 is arranged on the side wall of the right end of the filter bin 1 in a penetrating way, a support 7 in a cross structure is arranged at one end of the push rod 8 positioned at the inner side of the filter bin 1, the length of the bracket 7 is slightly smaller than the diameter of the hollow sleeve 31 with the largest diameter, the sleeve can slide under the pushing of the bracket 7, the side wall of the right end of the filter bin 1 is provided with a through hole 9 for the push rod 8 to pass through, a first sealing ring 10 is stuck on the inner wall of the through hole 9, a push plate 11 is arranged at one end of the push rod 8 positioned at the filter bin 1, in order to avoid the spring 36 from being reset under the impact action of water flow when the spring 36 is in elastic fatigue or the hollow sleeve 31 is blocked during filtering, the hollow sleeve 31 and the solid sleeve 32 can be pushed by manually pushing the push rod 8 to make the bracket 7 on the end part of the push rod push the hollow sleeve 31 and the solid sleeve 32, the sleeve is reset, so that the hollow sleeve 31 can be quickly cleaned manually;
the inner wall of the filtering bin 1 is provided with a flow guide notch II 28 communicated with the top end of the connecting pipe I19, the filtering bin 1, the filtering structure 3 and the push rod 8 are all inclined and arranged in parallel, the flow guide notch II 28 is arranged at the lower end of the filtering bin 1, the liquid inlet 6 is arranged at the high end of the filtering bin 1, the support 7 is parallel to the annular surface of the sleeve, and the inclined arrangement of the filtering bin 1 can accelerate the flow speed of water flow at the inner side of the filtering bin, so that the filtering efficiency of the filtering bin 1 on the bacillus subtilis fermentation liquid is improved, and meanwhile, the gathering amount of the bacillus subtilis fermentation liquid at the water inlet port of the filtering bin 1 can be reduced;
the concentration mechanism comprises a concentration bin 2, an ultrafiltration membrane 17 for filtering bacillus subtilis is arranged on the concentration bin 2, a liquid outlet and a waste discharge port 25 are also arranged on the concentration bin 2, a first connecting pipe 19 for communicating the concentration bin 2 is also arranged on the filtering bin 1, the concentration bin 2 further comprises two first supporting plates 14 which are obliquely arranged and can guide liquid, second supporting plates 15, a channel 21 which is arranged on each supporting plate and is of a corrugated structure, and a flow guide groove body 16 which is fixedly connected on each supporting plate through bolts and covers the channel 21 on the flow guide groove body, the cross section of the flow guide groove body 16 is of a semi-annular structure, a flow channel which can be penetrated by the liquid and is of a corrugated structure is formed between the flow guide groove body 16 and the supporting plates, an ultrafiltration membrane 17 which covers the channel 21 on the flow guide groove body and is pressed on the flow guide groove body 16 in a pressing mode at the periphery is paved on each supporting plate, and a sealing gasket 18 is adhered on the end face, in contact with the corresponding ultrafiltration membrane 17, of each flow guide groove body 16, the joint between the ultrafiltration membrane 17 and the flow guide groove body 16 can be sealed through the sealing gasket 18, so that water flow is prevented from leaking to the outer side of the flow guide groove body 16 when flowing in the flow guide groove body 16;
the inclination directions of the first support plate 14 and the second support plate 15 are opposite, the bottom end of the first connecting pipe 19 is inserted into the highest end of the flow guide groove body 16 on the first support plate 14, the bottom end of the first support plate 14 is provided with a second connecting pipe 29 for guiding the liquid at the lowest end of the flow guide groove body 16 to the second support plate 15, the lower end of the first support plate 14 is provided with a communicating port 23 communicated with the second connecting pipe 29, the bottom end of each support plate is provided with a flow guide plate 22 for guiding the liquid passing through the corresponding ultrafiltration membrane 17, the highest end of each flow guide groove body 16 is provided with an insertion hole 27 for inserting the bottom end of the corresponding connecting pipe, and a second sealing ring 26 for sealing between the connecting pipe and the flow guide groove body 16 is adhered to each insertion hole 27;
the second connecting pipe 29 penetrates through the guide plate 22, another inserting hole 27 for the bottom end of the first connecting pipe 19 to vertically penetrate is arranged on the guide plate 22 positioned below the first supporting plate 14, and the plug hole 27 is provided with another second sealing ring 26, the section of the guide plate 22 is arranged to be an annular structure, and each guide plate 22 is parallel to the corresponding support plate, and both ends of each guide plate 22 are fixedly connected with the bottom end of the corresponding support plate, the guide plates 22 are in a ring-shaped structure and are arranged in parallel with the corresponding support plates, so that the guide plates 22 can guide the solution passing through the bacillus subtilis rods of the support plates in two directions, so that the bacillus subtilis solution falling on the guide plate 22 is concentrated at the lower end of the arc of the guide plate 22, the bacillus subtilis solution can flow along the length direction of the guide plate 22 at the lower end of the arc of the guide plate 22 and finally flow to the lower end of the guide plate 22, so that the gathering effect of the guide plate 22 on the bacillus subtilis solution is improved;
the liquid outlet is provided with two, and is equipped with liquid outlet one 20 between the guide plate 22 on backup pad one 14 and its bottom to be equipped with liquid outlet two 24 between backup pad two 15 and the guide plate 22 on its bottom, liquid outlet one 20, liquid outlet two 24 all are located the below of corresponding backup pad low-end department, offer on the lateral wall in concentrated storehouse 2 and be used for the waste liquid exhaust's on backup pad two 15 waste discharge port 25.
The specific application process of the bacillus subtilis fermentation liquor concentration device is as follows: when the device is used, when bacillus subtilis fermentation liquor enters the filter bin 1 through the liquid inlet 6, weed fragments or other impurities in the fermentation liquor can be filtered through the hollow sleeves 31 which are sequentially sleeved and connected in a sliding mode, and when more fermentation liquor flows into the inner sides of the hollow sleeves 31 at one time, the fermentation liquor can impact the solid sleeves 32 with larger force, so that the multistage hollow sleeves 31 can slide under the dragging of the solid sleeves 32, the expansion of the multistage hollow sleeves 31 is realized, the filtering area of the filtering structure 3 on the fermentation liquor is increased, and the filtering efficiency of the filtering structure 3 is improved;
the liquid passing through the filtering structure 3 is guided to the first connecting pipe 19 through the second guiding groove 28, and the liquid can be conveyed to the highest position of the first guiding groove body 16 on the first supporting plate 14 through the first connecting pipe 19, the liquid flows along the first guiding groove body 14 in a circuitous manner when entering the inner side of the first guiding groove body 16, the bacillus subtilis is filtered by the ultrafiltration membrane 17 on the first supporting plate 14 when the liquid flows on the first guiding groove body 16 and falls onto the guiding plate 22 at the bottom end of the first supporting plate 14 through the channel 21 on the first supporting plate 14, the filtered bacillus subtilis solution can be guided and conveyed and discharged from the first liquid outlet 20 through the guiding plate 22, and the liquid flowing to the lower end of the first supporting plate 14 can flow into the second supporting plate 15 through the second connecting pipe 29 again, so that the circuitous guiding groove body 16 on the second supporting plate 15 can guide the liquid again, the filtration of the bacillus subtilis in the liquid is realized in the process of the guide flow, the bacillus subtilis solution which is filtered out for the second time passes through the second support plate 15 and falls into the guide plate 22 below the second support plate, and is finally discharged from the second liquid outlet 24, and the liquid which is on the second support plate 15 and is not filtered is discharged from the waste discharge port 25;
in addition, when the hollow sleeve 31 slides under the action of impact force, the spring 36 is compressed, the hollow sleeve 31 can be reset and powered by the elastic resetting capacity of the spring 36, namely when the acting force of water flow impact on the hollow sleeve 31 is reduced, the hollow sleeve 31 can be reset under the elastic action of the spring 36, and when the hollow sleeve 31 is reset, the inner side wall of the hollow sleeve 31 on the corresponding side can be attached to the sealing ring four 310 in a clinging manner, impurities attached to the inner wall of the hollow sleeve 31 can be scraped, so that self-cleaning of the hollow sleeve 31 is realized, the hollow sleeve 31 can still have good filtering performance when being used next time, the impurities scraped by the sealing ring four 310 can fall onto the inner wall of the first flow guide notch 12, the filtered impurities are guided to the inside of the collecting tank 13 through the first flow guide notch 12 and are connected with the filtering tank 1 through screws, can make things convenient for the clearance of staff later stage to the inside impurity of collecting vat 13, and in order to avoid spring 36 to take place elasticity fatigue or fretwork sleeve 31 to block up when filtering and spring 36 this moment can not take place to reset under the impact of rivers, accessible manual promotion push rod 8 makes support 7 on its tip can be to fretwork sleeve 31 this moment, solid sleeve 32 promotes, reset with this help sleeve, thereby the manual quick clearance to fretwork sleeve 31 of realization of accessible, the use of this bacillus subtilis zymotic fluid enrichment facility has been accomplished so.
It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that changes and modifications to the above embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.
Claims (9)
1. The utility model provides a bacillus subtilis zymotic fluid enrichment facility, includes filtering mechanism and concentration mechanism, filtering mechanism includes crosses filter bin (1) and locates the inside filtration (3) in filter bin (1), it is equipped with inlet (6) on filter bin (1), concentration mechanism includes concentration bin (2), be equipped with on concentration bin (2) and carry out filterable milipore filter (17) to bacillus subtilis, and still be equipped with liquid outlet, waste discharge mouth (25) on concentration bin (2), a serial communication port, still install connecting pipe (19) that are used for communicateing concentration bin (2) on filtering bin (1), concentration bin (2) still include two slope settings and can carry out the water conservancy diversion to liquid backup pad one (14), backup pad two (15), open and be ripple structure's passageway (21) in every backup pad, through bolt fixed connection in every backup pad and to the water conservancy diversion cell body (16) that its upper channel (21) sheltered from, the section of the flow guide groove body (16) is of a semi-ring structure, a flow channel which can be penetrated by liquid and is of a wave structure is formed between the flow guide groove body (16) and the supporting plates, and an ultrafiltration membrane (17) which covers the channel (21) on the supporting plate and is pressed on the periphery of the flow guide groove body (16) is laid on each supporting plate;
the inclination directions of the first support plate (14) and the second support plate (15) are opposite, the bottom end of the first connecting pipe (19) is inserted into the highest end of the flow guide groove body (16) on the first support plate (14), the bottom end of the first support plate (14) is provided with a second connecting pipe (29) which guides the liquid at the lowest end of the flow guide groove body (16) on the first support plate to the second support plate (15), the lower end of the first support plate (14) is provided with a communicating port (23) communicated with the second connecting pipe (29), and the bottom end of each support plate is provided with a flow guide plate (22) which guides the liquid passing through the corresponding ultrafiltration membrane (17);
the liquid outlet is provided with two, and is equipped with liquid outlet one (20) between backup pad one (14) and its guide plate (22) on the bottom to be equipped with liquid outlet two (24) between backup pad two (15) and its guide plate (22) on the bottom, liquid outlet one (20), liquid outlet two (24) all are located the below of corresponding backup pad low-end department, offer on the lateral wall in concentrated storehouse (2) and be used for waste liquid exhaust's waste discharge mouth (25) on backup pad two (15).
2. The bacillus subtilis fermentation liquor concentration device according to claim 1, wherein the filtering structure (3) comprises hollow sleeves (31) which are sequentially connected in a sleeved manner, every two adjacent hollow sleeves (31) are movably connected through a sliding structure, a connecting block (37) connected with a water inlet of the filtering bin (1) is arranged on the peripheral circumference of the hollow sleeve (31) with the largest diameter, the hollow sleeves (31), the filtering bin (1) and the connecting block (37) are all of rectangular structures, an installation notch (4) used for installing the connecting block (37) is formed in the water inlet of the filtering bin (1), a sealing ring III (38) is adhered to the end face of the connecting block (37) in contact with the installation notch (4), and through holes (39) for filtering a solution containing bacillus subtilis are formed in the peripheral circumference of each hollow sleeve (31) at equal intervals, solid sleeve (32) that the diameter is minimum have solid sleeve (32) through another sliding construction swing joint, filter the water inlet of storehouse (1) and go up screw connection and have apron (5), and liquid inlet (6) are located on apron (5).
3. The bacillus subtilis fermentation liquor concentration device according to claim 2, wherein the sliding structure comprises strip-shaped sliding grooves (34) which are equidistantly arranged on the peripheral circumference of each sleeve, sliding blocks (33) which are slidably arranged on the inner side of each strip-shaped sliding groove (34) and the other ends of which are fixedly connected with the inner wall of the corresponding side sleeve, a limiting rod (35) which is parallel to the inner side of each strip-shaped sliding groove (34) is arranged on the inner side of each strip-shaped sliding groove (34), each limiting rod (35) penetrates through the corresponding sliding block (33) and is slidably connected with the sliding block (33), and a roller (311) which is in contact with the strip-shaped sliding grooves (34) is arranged on each sliding block (33).
4. The bacillus subtilis fermentation liquor concentration device according to claim 3, wherein each limiting rod (35) is sleeved with a spring (36) which elastically supports the corresponding side sliding block (33), all sleeves are positioned on the inner side of the sleeve with the largest diameter when the spring (36) is in a natural state, a sealing ring four (310) is arranged between every two adjacent sleeves, each sealing ring four (310) is arranged on a peripheral ring at the left end of the corresponding sleeve, the peripheral ring of each sealing ring four (310) is used for scraping chips on the inner wall of the corresponding sleeve, the bottom end of the installation notch (4) is provided with a flow guide notch I (12) which receives the chips scraped from the inner wall of the sleeve, and the bottom end of the filter bin (1) is inserted with a collecting tank (13) communicated with the flow guide notch I (12), and the collecting groove (13) is connected with the filtering bin (1) through screws.
5. The bacillus subtilis fermentation liquor concentration device according to claim 4, wherein a push rod (8) is arranged at the right end side wall of the filter bin (1) in a penetrating manner, a support (7) in a cross structure is arranged at one end of the push rod (8) positioned at the inner side of the filter bin (1), the length of the support (7) is slightly smaller than the diameter of a hollow sleeve (31) with the largest diameter, the sleeve can slide under the pushing of the support (7), a through hole (9) for the push rod (8) to pass through is formed in the right end side wall of the filter bin (1), a first sealing ring (10) is bonded on the inner wall of the through hole (9), and a push plate (11) is arranged at one end of the push rod (8) positioned at the filter bin (1).
6. The bacillus subtilis fermentation broth concentrating device according to claim 1, wherein the highest end of each guide groove body (16) is provided with a plug hole (27) for plugging the bottom end of the corresponding connecting pipe, and each plug hole (27) is adhered with a second sealing ring (26) for sealing between the connecting pipe and the guide groove body (16).
7. The bacillus subtilis fermentation liquor concentration device according to claim 1, wherein the second connecting pipe (29) penetrates through the guide plate (22), another inserting hole (27) for the bottom end of the first connecting pipe (19) to vertically penetrate through is formed in the guide plate (22) below the first supporting plate (14), another sealing ring second (26) is arranged on the inserting hole (27), the section of the guide plate (22) is arranged to be in an annular structure, each guide plate (22) is parallel to the corresponding supporting plate, and two ends of each guide plate (22) are fixedly connected with the bottom end of the corresponding supporting plate.
8. The bacillus subtilis fermentation liquor concentration device according to claim 5, wherein a second diversion notch (28) communicated with the top end of the first connecting pipe (19) is formed in the inner wall of the filtering bin (1), the filtering structure (3) and the push rod (8) are all arranged in parallel, the second diversion notch (28) is formed in the lower end of the filtering bin (1), the liquid inlet (6) is formed in the high end of the filtering bin (1), and the support (7) is parallel to the annular surface of the sleeve.
9. The bacillus subtilis fermentation liquor concentration device according to claim 1, wherein a sealing gasket (18) is adhered to the end surface of each flow guide groove body (16) which is in contact with the corresponding ultrafiltration membrane (17).
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| CN202110111085.0A CN112915634B (en) | 2021-01-27 | 2021-01-27 | Bacillus subtilis fermentation liquid concentration device |
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| CN112915634B true CN112915634B (en) | 2022-04-22 |
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| JP2002058918A (en) * | 2000-08-18 | 2002-02-26 | Marukou Tekkosho:Kk | Waste water treating equipment |
| CN1623636A (en) * | 2004-10-26 | 2005-06-08 | 贡有成 | Folding tubing film and filter formed by said tubing film |
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