CN112915281A - Anticoagulant-free PRP biological membrane preparation device and method - Google Patents
Anticoagulant-free PRP biological membrane preparation device and method Download PDFInfo
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- CN112915281A CN112915281A CN202110273716.9A CN202110273716A CN112915281A CN 112915281 A CN112915281 A CN 112915281A CN 202110273716 A CN202110273716 A CN 202110273716A CN 112915281 A CN112915281 A CN 112915281A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
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Abstract
The application discloses a PRP biological membrane preparation device and method without anticoagulant. On one hand, the anticoagulant-free PRP biological membrane preparation device comprises a disc body, wherein a cavity is formed in the disc body, and a plurality of burr protrusions are uniformly formed on the bottom surface of the cavity; a pipe joint penetrating through the side wall of the cavity is arranged on the tray body, a valve is arranged on the pipe joint, and the pipe joint is connected with the blood taking needle in an inserting manner; the tray body is sealed and covered with a medical protective film; a socket communicated to the inner wall of the cavity is arranged at the notch of the cavity; the socket is detachably connected with the negative suction mechanism in an inserted manner. On the other hand, the application provides a preparation method of the anticoagulant-free PRP biological membrane, which comprises the processes of assembling, blood sampling, standing blood coagulation and the like of the anticoagulant-free PRP biological membrane preparation device, and is simple to operate. The PRP biological membrane preparation device and the method without the anticoagulant can be used for preparing the PRP biological membrane with high efficiency and low cost.
Description
Technical Field
The application relates to the field of PRP (platelet-rich plasma) biomembrane preparation devices, in particular to a PRP biomembrane preparation device and method without an anticoagulant.
Background
At present, in the treatment process of intractable wounds, platelet-rich (PRP) biofilms are often used for clinical treatment.
At present, the preparation methods of the platelet-rich biofilm commonly used are mainly two types: one is to collect the blood of a patient, avoid blood coagulation by adding anticoagulant such as sodium citrate, separate the collected blood by a centrifugal separator to obtain a platelet layer, then add coagulant such as calcium chloride, and spread the liquid into a film shape to form a platelet-rich biomembrane after coagulation; the other is to collect blood from a patient, the blood is naturally coagulated by the coagulation factor of the blood itself, a coagulated clot is collected by a sterile forceps, and the clot is mechanically compressed into a film shape. The two methods are relatively complex to operate, require a long time, and have relatively high equipment cost.
Disclosure of Invention
In order to solve the problems, the application provides a device and a method for preparing a PRP biological membrane without an anticoagulant.
In one aspect, the present application provides an anticoagulant-free PRP biofilm manufacturing apparatus including a tray body, wherein,
the tray body is provided with a cavity, the depth of the cavity is within the range of 8mm-10mm, the length and the width of the cavity are within the range of 40mm-60mm, a plurality of burr protrusions are uniformly arranged on the bottom surface of the cavity, and the distance between the burr protrusions is 5 mm;
a pipe joint penetrating through the side wall of the cavity is arranged on the tray body, a valve is arranged on the pipe joint, and the pipe joint is connected with the blood taking needle in an inserting manner;
the tray body is sealed and covered with a medical protective film;
a socket communicated to the inner wall of the cavity is arranged at the notch of the cavity;
the socket is detachably connected with the negative suction mechanism in an inserted manner.
Preferably, the pipe joint is provided at a corner of the tray body, and the socket is provided at a diagonal of the corner.
Preferably, the negative suction mechanism comprises a needle cylinder and a control joint, the control joint comprises a three-way joint, the needle cylinder is communicated with one end of the three-way joint, one-way valve is arranged at the other two ends of the three-way joint, one-way valve is communicated with the needle cylinder, the other one-way valve is communicated with the three-way joint, the other one-way valve is communicated with the outside of the three-way joint, and one end of the three-way joint, which is provided with the one-way valve and is directed to the needle cylinder.
Preferably, a circumferential groove is formed in the top of the side wall of the tray body.
Preferably, the tray body is a transparent structure formed by medical polypropylene, and scales are arranged on the side wall of the tray body.
Preferably, the medical protective film is a medical polypropylene film.
In another aspect, the present application provides a method for preparing a PRP biofilm without an anticoagulant, and an apparatus for preparing a PRP biofilm using the same, comprising:
connecting the blood taking needle to a pipe joint, covering the cavity of the tray body by a medical protective film, connecting the control joint to the socket, and connecting the needle cylinder to the control joint;
sterilizing the blood taking needle, and inserting the blood taking needle into a vein of a patient;
opening the valve, and repeatedly pumping and pressing the needle cylinder to enable the blood taking needle to draw blood from the vein of the patient until a set blood taking amount is reached;
and closing the valve to take down the blood taking needle, and standing until the blood is coagulated into a PRP biological membrane.
The preparation device and method of the anticoagulant-free PRP biological membrane have the following beneficial effects:
the application provides a PRP biomembrane preparation facilities of no anticoagulant passes through medical protecting film with the cavity of disk body forms relative confined sterile environment, through set up the cylinder and the control joint that are used for producing the negative pressure on the disk body come produce certain negative pressure in the cavity, blood sampling from the patient's vein through the blood taking needle of connecting at the coupling arrives in the cavity until reaching 3mm, will valve on the coupling is closed and is stood and wait for blood to coagulate under the coagulation factor effect that self contains, in the coagulation process, in the cavity burr arch can separate the coagulation fibre in the blood, avoids coagulation fibre to gather into a group when coagulating and cause the problem that blood coagulates into the piece in the coagulation process, consequently the PRP biomembrane preparation facilities of no anticoagulant that this application provided can guarantee that blood evenly coagulates into comparatively even membrane, rolling is not required. Moreover, the anticoagulant-free PRP biological membrane preparation device is simple in structure, low in cost and low in material cost. On the other hand, the preparation method of the anticoagulant-free PRP biological membrane provided by the application utilizes the anticoagulant-free PRP biological membrane preparation device provided by the application, blood is directly collected from the vein of a patient to the cavity, the cavity is stood for coagulation, the degree of centrifugation and coagulation agglomeration is low without adding the anticoagulant, the clot is not required to be rolled, and the operation flow for preparing the PRP biological membrane is simple, the time is short, the efficiency is high, and the cost is low.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a structural view of a PRP biofilm formation apparatus without an anticoagulant in the example of the present application;
FIG. 2 is a cross-sectional view of a control joint in an embodiment of the present application;
FIG. 3 is a flow chart of a method for preparing an anticoagulant-free PRP biofilm according to an embodiment of the present application.
The reference numbers and meanings in the figures are as follows:
1. disk body, 11, cavity, 12, burr arch, 13, socket, 14, valve, 15, recess, 2, medical protective film, 3, blood taking needle, 4, syringe, 5, control joint.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The present invention will be described with reference to the accompanying drawings, in which FIG. 1 is a schematic diagram of an anticoagulant-free PRP biofilm preparation apparatus according to an embodiment of the present invention; FIG. 2 is a cross-sectional view of a control joint in an embodiment of the present application;
FIG. 3 is a flow chart of a method for preparing an anticoagulant-free PRP biofilm according to an embodiment of the present application.
Referring to fig. 1, the invention provides a PRP biofilm preparation device without anticoagulant, which comprises a tray body 1, wherein a cavity 11 is arranged on the tray body 1, and a plurality of burr protrusions 12 are uniformly arranged on the bottom surface of the cavity 11; in the specific implementation process, the depth of the cavity 11 is within the range of 8mm-10mm, the length and the width of the cavity are within the range of 40mm-60mm, the distance between the burr protrusions is 5mm, and a feasible shape of the burr protrusions is conical, the diameter of the bottom surface of the burr protrusions is 2mm-3mm, and the height of the burr protrusions is 3 mm.
Set up on the disk body 1 and run through the coupling of 11 lateral walls of cavity, in the concrete implementation process, the coupling is close to a corner of disk body 1, be provided with valve 14 on the coupling, the coupling is pegged graft and is communicate blood taking needle 3.
A socket 13 communicated to the inner wall of the cavity 11 is arranged at the notch of the cavity 11, and the height of the communicated part of the socket 13 and the inner wall of the cavity 11 relative to the bottom of the cavity 11 is higher than 3 mm; the socket 13 is detachably connected with a negative suction mechanism. In a specific implementation, the insertion openings 13 are arranged at opposite corners of the pipe joint. The negative suction mechanism comprises a medical needle cylinder 4 and a control joint 5, as shown in the figure 2, the control joint 5 comprises a main body formed by a three-way joint, the needle cylinder 4 is detachably inserted into one end of the three-way joint, the other two ends of the three-way joint are respectively provided with a one-way valve, one of the one-way valves leads to the needle cylinder 4 and is communicated with the socket 13, and the other one-way valve leads to the outside. When the needle cylinder 4 is used for air suction, the needle cylinder 4 is communicated with the cavity 11 through the one-way valve of the control joint 5, and the needle cylinder 4 is isolated from the outside; when the needle cylinder 4 exhausts, the needle cylinder 4 is isolated from the cavity by the one-way valve of the control joint 5, and the needle cylinder is communicated with the outside.
The medical protective film 2 is hermetically covered on the tray body 1; in the specific implementation process, a groove 15 is formed in the top of the side wall of the tray body 1, and the groove 15 is used for arranging and fixing a rubber band or a bandage of the medical protective film 2. The medical protective film 2 is a medical polypropylene film.
In the specific implementation process, the tray body 1 is a transparent structure formed by medical polypropylene, the side wall of the tray body 1 is provided with scales, and the zero point of the scales is opposite to the bottom surface of the cavity 11.
In another aspect, referring to fig. 3, the present application provides a method for preparing a PRP biofilm without an anticoagulant, and an apparatus for preparing a PRP biofilm using the same, comprising:
s1, assembling the PRP biological membrane preparation device without the anticoagulant. Specifically, a blood taking needle is connected with a pipe joint, a cavity of a disc body is covered by a medical protective film, a control joint is connected with a socket, a needle cylinder is connected with the control joint, and the disc body is horizontally arranged;
and S2, closing the valve on the pipe joint, and pre-pumping the syringe to generate a certain slight negative pressure in the cavity of the disc body.
And S3, sterilizing the blood taking needle, and inserting the blood taking needle into the vein of the patient.
And S4, opening the valve, enabling the blood to flow out under the action of negative pressure, and repeatedly pumping and pressing the needle cylinder to enable the blood taking needle to draw blood from the vein of the patient until the set blood taking amount is reached, wherein the thickness of the blood taken in the cavity is controlled to be 3mm by the general blood taking amount.
And S5, closing the valve to remove the blood taking needle from the body of the patient, and standing until the blood is coagulated into PRP biomembranes.
S6, opening the medical protective membrane, and taking out the PRP biological membrane through medical forceps.
Before step S6, if it is necessary to remove red blood cells from the generated PRP biofilm, the blood collection needle is connected to a saline bottle or a saline bag, and the syringe is pumped to suck saline to flush the PRP biofilm, and the burred protrusion can fix the PRP biofilm to prevent the PRP biofilm from being flushed away by the saline. After the washing is finished, the control joint is taken down from the socket, the blood taking needle is taken down from the pipe joint, and washing liquid is discharged from the pipe joint.
The anticoagulant-free PRP biomembrane preparation device provided by the invention forms a relatively closed sterile environment through the medical protective membrane 2 and the cavity 11 of the tray body 1, generates certain negative pressure in the cavity 11 by arranging the needle cylinder 4 and the control joint 5 which are used for generating negative pressure on the tray body 1, collects blood from the vein of a patient into the cavity until reaching 3mm through the blood collection needle 3 connected with the joint, closes and stands the valve 14 on the joint to wait for the blood to coagulate under the action of coagulation factors contained in the blood, and the burr bulge 12 in the cavity 11 can separate coagulation fibers in the blood in the coagulation process, so that the problem that the coagulation fibers are agglomerated during coagulation to cause the coagulation of the blood into blocks in the coagulation process is avoided, therefore, the anticoagulant-free PRP biomembrane preparation device provided by the invention can ensure that the blood is uniformly coagulated into a relatively uniform membrane, rolling is not required. Moreover, the anticoagulant-free PRP biological membrane preparation device is simple in structure, low in cost and low in material cost. On the other hand, the preparation method of the anticoagulant-free PRP biological membrane provided by the application utilizes the anticoagulant-free PRP biological membrane preparation device provided by the application, blood is directly collected from the vein of a patient to the cavity, the cavity is stood for coagulation, the degree of centrifugation and coagulation agglomeration is low without adding the anticoagulant, the clot is not required to be rolled, and the operation flow for preparing the PRP biological membrane is simple, the time is short, the efficiency is high, and the cost is low.
It should be noted that in the claims, any reference signs placed between parentheses shall not be construed as limiting the claim. The word "comprising" does not exclude the presence of elements or steps not listed in a claim. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements. The invention may be implemented by means of hardware comprising several distinct elements, and by means of a suitably programmed computer. In the unit claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The usage of the words first, second and third, etcetera do not indicate any ordering. These words may be interpreted as names.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following appended claims be interpreted as including the preferred embodiment and all such alterations and modifications as fall within the true scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.
Claims (7)
1. A PRP biofilm preparation device without anticoagulant, which is characterized by comprising a disc body (1), wherein,
a cavity (11) is formed in the tray body (1), and a plurality of burr protrusions (12) are uniformly arranged on the bottom surface of the cavity (11);
a pipe joint penetrating through the side wall of the cavity (11) is arranged on the tray body (1), a valve (14) is arranged on the pipe joint, and the pipe joint is connected with the blood taking needle (3) in an inserting manner;
the medical protective film (2) is hermetically covered on the tray body (1);
a socket (13) communicated to the inner wall of the cavity (11) is arranged at the notch of the cavity (11);
the socket (13) is detachably connected with a negative suction mechanism.
2. The anticoagulant-free PRP biofilm preparation device according to claim 1, wherein the pipe joint is provided at a corner of the tray body (1) and the socket (13) is provided at a diagonal corner of the corner.
3. The anticoagulant-free PRP biofilm preparation device according to claim 1, wherein the negative suction mechanism comprises a needle cylinder (4) and a control joint (5), the control joint (5) comprises a three-way joint, the needle cylinder (4) is communicated with one end of the three-way joint, one check valve is arranged at the other two ends of the three-way joint, one check valve is opened towards the needle cylinder (4), the other check valve (4) is opened towards the outside of the three-way joint, and one end of the three-way joint, which is provided with the check valve and is directed towards the needle cylinder (4), is communicated with the socket (13).
4. The anticoagulant-free PRP biofilm preparation device according to claim 1, wherein a circumferential groove (15) is provided at the top of the side wall of the tray body (1).
5. The anticoagulant-free PRP biofilm preparation device according to claim 1, wherein the tray body (1) is a transparent structure formed by medical polypropylene, and scales are arranged on the side wall of the tray body (1).
6. The anticoagulant-free PRP biofilm preparation device according to claim 1, wherein the medical protective membrane (2) is a medical polypropylene film.
7. A method for preparing a non-anticoagulant PRP biofilm using the non-anticoagulant PRP biofilm preparation device according to any one of claims 1 to 6, comprising:
connecting the blood taking needle to a pipe joint, covering the cavity of the tray body by a medical protective film, connecting the control joint to the socket, and connecting the needle cylinder to the control joint;
sterilizing the blood taking needle, and inserting the blood taking needle into a vein of a patient;
opening the valve, and repeatedly pumping and pressing the needle cylinder to enable the blood taking needle to draw blood from the vein of the patient until a set blood taking amount is reached;
and closing the valve to take the blood taking needle from the patient, and standing until the blood is coagulated into PRP biomembranes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110273716.9A CN112915281B (en) | 2021-03-12 | 2021-03-12 | Preparation device and method of anticoagulant-free PRP (platelet-Rich plasma) biomembrane |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110273716.9A CN112915281B (en) | 2021-03-12 | 2021-03-12 | Preparation device and method of anticoagulant-free PRP (platelet-Rich plasma) biomembrane |
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| CN112915281A true CN112915281A (en) | 2021-06-08 |
| CN112915281B CN112915281B (en) | 2022-12-16 |
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| CN112295017A (en) * | 2020-10-28 | 2021-02-02 | 武汉齿欣生物医药科技有限责任公司 | Optimized preparation method of solid platelet-rich fibrin membrane |
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| WO1995026749A1 (en) * | 1994-03-31 | 1995-10-12 | Fibrin Corporation | Cryoprecipitated native fibrinogen concentrates |
| CN1297356A (en) * | 1998-02-27 | 2001-05-30 | 拜奥拉拜奥爱克斯公司 | Matrix protein compositions for wound healing |
| CN103415329A (en) * | 2011-03-11 | 2013-11-27 | 汾沃有限公司 | Membrane separation devices, systems and methods employing same, and data management systems and methods |
| EP2574350A1 (en) * | 2011-09-29 | 2013-04-03 | Biorigen S.r.l | Medical uses of lyophilized polymeric membranes containing blood derivatives |
| CN103690999A (en) * | 2013-12-10 | 2014-04-02 | 北京大学口腔医学院 | Implant material with PRF precursor liquid solidified into gel mask |
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| CN112915281B (en) | 2022-12-16 |
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