CN112914503B - Method, apparatus, storage medium, and electronic device for measuring wound of subject - Google Patents
Method, apparatus, storage medium, and electronic device for measuring wound of subject Download PDFInfo
- Publication number
- CN112914503B CN112914503B CN201911238458.XA CN201911238458A CN112914503B CN 112914503 B CN112914503 B CN 112914503B CN 201911238458 A CN201911238458 A CN 201911238458A CN 112914503 B CN112914503 B CN 112914503B
- Authority
- CN
- China
- Prior art keywords
- skin
- data
- intensity
- subject
- raman signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
- A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
- A61B5/445—Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Heart & Thoracic Surgery (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Veterinary Medicine (AREA)
- Medical Informatics (AREA)
- Biophysics (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Dermatology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a method, a device and an electronic device for measuring a wound of a subject, wherein the method comprises the following steps: obtaining first data relating to a thickness of an epidermal layer of skin of a site to be examined of a subject; receiving a first Raman signal from skin of the site to be measured of the subject; obtaining second data according to the first Raman spectrum signal, wherein the second data is related to the content of the repair component in the dermis layer of the skin of the part to be detected; and determining the skin wound characteristics of the part to be detected of the detected object according to the first data and the second data. The wound repair response can be rapidly identified through Raman spectroscopy, and more scientific understanding and judgment of the wound response can be prompted to doctors, so that the method provides help for further methods and drug selection of medical treatment. The technical scheme of the invention is non-invasive, and the wound is detected non-invasively, so that the pain of the patient is relieved.
Description
Technical Field
The invention relates to the field of medical treatment, in particular to a method, a device, a storage medium and an electronic device for measuring a wound of a subject.
Background
Chronic wounds are a major health problem in the world and comprise a range of underlying diseases, with wounds taking the form of three major conditions: bedsores, venous ulcers and diabetic ulcers. Chronic and difficult healing wounds often lead to serious distress and disability in the elderly, while also adding a significant amount of work to the healthcare workers (clinical and laboratory tests).
The primary treatment for chronic wounds is with frequent and long lasting dressing changes and/or surgery of the wound. The treatment method of changing the medicine is characterized in that the etiology of the chronic wound is unclear, the thought of a doctor is to adopt the medicine to carry out the treatment by inspection when changing the medicine, and the medicine selection determined by the effect of the change of the wound after changing the medicine for a plurality of times is changed or not changed, so that the difference recognition that 'the kernel sees the kernel and the intelligence of the intelligence sees the intelligence' is objective. The main operation of the chronic wound surface operation is debridement, and correct removal of chronic ulcers is very important for achieving good clinical effects. Global data indicate that debridement of patients is typically performed weekly, and that debridement at the correct time has been shown to increase the rate of healing of venous ulcers (one of three slow-wound conditions) compared to the rate of healing of non-debrided wounds (the prescription change method). Between 8 and 20 weeks after debridement, the debrided ulcer healing rate accounted for 16%, the non-debrided ulcer accounted for 4.3%, and about 20% of patients never healed. From these data, it was also found that the cost of treatment for chronic wounds exceeds $ 250 billion per year in the united states alone, which also includes the cost of surgical debridement (the primary means of treating chronic wounds).
Compared with tumor resection and other surgical operations, the recognition of the chronic wound debridement operation is relatively original, and still stays at the cognitive level of the acute wound debridement operation (the textbook does not have the content of the chronic wound, even if a student course is researched, the chronic wound is a new subject which develops and evolves along with the economic development in recent decades). Although some common clinical/pathological factors may help to determine in advance whether a wound is "healing" or "non-healing", or an acute wound fails to heal normally and turns into a chronic wound, none of these refractory wounds have had any specific laboratory test to distinguish and define objective visual descriptions and explanations of the refractory wound types, rather than the physician's guesses. In addition, the chronic wound debridement also has no objective histology at present, biological and molecular markers can help clinicians make accurate judgment on wounds in real time, and even so far, most new surgeons are still taught that the debridement till bleeding is the so-called gold standard judgment of thorough debridement and the important decision debridement principle of entering the next step of treatment. Furthermore, to date, there is no clear way to define how to predict the healing process and the likely response of a patient to wound care treatment.
Wound healing is controlled by complex biological processes involving multiple cell types. The "over-stimulation" or "under-stimulation" of the response to the healing phase of skin injury may result in poor healing of the wound. The healing process of skin lesions includes these phases: coagulation, inflammation, angiogenesis, followed by fibrosis for collagen synthesis, wound contractile epithelialization and finally tissue remodeling.
Therefore, the real-time visualization technology and method adopted for supplementing objective and rapid methods to the healing capacity and healing process of the wound, especially the chronic wound, can provide comprehensive objective difference evidence for the cognition of doctors to different patients and even different wounds of the same patient, thereby providing better treatment measures for the patient through 'customized' and helping the patient to reduce unnecessary expensive treatment cost to a certain extent.
Disclosure of Invention
In order to solve the above problem, embodiments of the present invention provide a method, an apparatus, and an electronic device for measuring a wound of a subject.
In a first aspect, an embodiment of the present invention provides a method for measuring a wound of a subject, including the following steps:
obtaining first data relating to a thickness of an epidermal layer of skin of a site to be measured of a subject;
receiving a first Raman signal from skin of the site to be measured of the subject;
obtaining second data according to the first Raman signal, wherein the second data is related to the content of the repair component in the dermis layer of the skin of the part to be detected;
and determining the skin wound characteristics of the part to be detected of the detected object according to the first data and the second data.
Optionally, the obtaining the first data comprises:
receiving a second Raman signal from skin of an intact part of the subject, the intact part of the skin being proximal to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model.
Optionally, the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～ 1850cm -1 Selected from within.
Optionally, the first prediction model satisfies the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the first data.
Optionally, the method of determining the first set of constants comprises:
collecting a plurality of said ratios of different body parts of at least one subject;
measuring the skin thickness of each of the different body parts;
generating a calibration curve relating ratio to skin thickness from said ratio and resulting skin thickness for each of said different body parts;
determining the first set of constants from the calibration curve.
In a second aspect, embodiments of the present invention provide another method of measuring a wound of a subject, comprising the steps of:
obtaining first data relating to a thickness of an epidermal layer of skin of a part to be measured of a subject;
receiving a third Raman signal from skin of the site to be measured of the subject at a first time;
obtaining second data according to the third Raman signal, wherein the second data is related to the content of the repair components in the dermis layer of the skin of the part to be detected at the first time;
determining a wound characteristic of the subject at a first time based on the first data and the second data;
receiving a fourth Raman signal from the skin of the site to be measured of the subject at a second time;
obtaining third data according to the fourth Raman signal, wherein the third data is related to the content of the repair components in the dermis layer of the skin of the part to be detected at a second time;
determining wound characteristics of the subject at a second time according to the first data and the third data;
determining a wound change characteristic of the subject based on the wound characteristics at the first time and the wound characteristics at the second time.
Optionally, the obtaining the first data comprises:
receiving a fifth raman signal from skin of an intact part of the subject, the intact part of the skin being proximal to the skin of the site to be measured;
measuring a first intensity of the fifth raman signal at a predetermined wavenumber and a second intensity of the fifth raman signal at a predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model.
Optionally, the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～ 1850cm -1 Selected from the list.
Optionally, the first prediction model satisfies the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the first data or the third data.
Optionally, the method of determining the first set of constants comprises:
collecting a plurality of said ratios of different body parts of at least one subject;
measuring the skin thickness of each of the different body parts;
generating a calibration curve relating ratio to skin thickness from said ratio and resulting skin thickness for each of said different body parts;
determining the first set of constants from the calibration curve.
In a third aspect, embodiments of the present invention provide an apparatus for measuring a wound of a subject, the apparatus comprising:
a first obtaining unit configured to obtain first data relating to a thickness of an epidermal layer of skin of a part to be measured of a subject;
a receiving unit configured to receive a first raman signal from skin of the portion to be measured of the subject;
a second obtaining unit configured to obtain second data based on the first raman signal, the second data being related to a content of a repair component in a dermis layer of the skin of the site to be measured;
a determination unit for determining a skin wound characteristic of the portion to be measured of the subject according to the first data and the second data.
In a fourth aspect, embodiments of the present invention provide an apparatus for measuring a wound of a subject, the apparatus comprising:
a first obtaining unit configured to obtain first data relating to a thickness of an epidermal layer of skin of a part to be measured of a subject;
a receiving unit configured to receive a third raman signal from the skin of the portion to be measured of the subject at a first time;
a second obtaining unit, configured to obtain second data according to the third raman signal, where the second data is related to a content of a repair component in a dermis layer of the skin of the site to be detected at a first time;
a determination unit for determining a wound characteristic of the subject at a first time from the first data and the second data;
the receiving unit is further used for receiving a fourth Raman signal from the skin of the part to be measured of the examinee at a second time;
the second obtaining unit is further configured to obtain third data according to the fourth raman signal, where the third data is related to a content of a repair component in a dermis layer of the skin of the site to be measured at a second time;
the determining unit is further used for determining wound characteristics of the examinee at a second time according to the first data and the third data;
a second determination unit for determining a wound change characteristic of the subject according to the wound characteristic at the first time and the wound characteristic at the second time.
In a fifth aspect, an embodiment of the present invention provides a detection system, including: the device comprises a laser, a probe, a spectrum analyzer and a transmission optical fiber;
the laser transmits laser to the probe through the transmission optical fiber, so that the probe enables the laser to irradiate the skin of the predetermined position of the detected object to excite a Raman signal;
the probe collects the Raman signal and transmits the Raman signal to the spectrum analyzer through the transmission optical fiber;
and the spectrum analyzer performs spectrum analysis on the Raman signal by the method to obtain the skin wound characteristics of the part to be detected of the detected person.
In a sixth aspect, the present invention provides a computer-readable storage medium, on which a computer program is stored, which when executed by a processor implements the steps of any one of the above methods.
In a seventh aspect, an embodiment of the present invention provides an electronic device, where the electronic device is configured to implement the steps of any one of the foregoing methods.
The method, the device and the electronic equipment for measuring the wound of the detected person are different from the method for observing the healing condition of the wound by the eyes of the operating doctor, determine the repair reaction of the wound through Raman spectrum, and prompt doctors to understand and judge the wound reaction more scientifically so as to provide help for further methods and medicine selection of medical treatment. The technical scheme of the invention is non-invasive, and the wound is detected non-invasively, so that the pain of the patient is relieved. Meanwhile, the technical scheme of the invention has good response to the wound of the surgical debridement, and can be used for determining the position for removing the chronic wound and/or the debridement degree to judge whether the debridement is thorough.
Drawings
FIG. 1 is a schematic flow chart diagram illustrating a method for determining a first set of constants in a first predictive model according to an embodiment of the present invention;
FIG. 2 is a schematic flow chart of a method for measuring epidermal skin thickness according to an embodiment of the present invention;
fig. 3 is a schematic flow chart of a method of measuring a wound of a subject according to an embodiment of the present invention;
FIG. 4 is a flow chart schematic diagram of another method of measuring a wound of a subject provided by an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of an apparatus for measuring a wound of a subject according to an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of another apparatus for measuring a wound of a subject according to an embodiment of the present invention;
fig. 7 is a schematic structural diagram of an electronic device according to an embodiment of the present invention.
Detailed Description
The present application is further described with reference to the following figures and examples.
In the following description, the terms "first" and "second" are used for descriptive purposes only and are not intended to indicate or imply relative importance. The following description provides embodiments of the invention, which may be combined with or substituted for various embodiments, and this application is therefore intended to cover all possible combinations of the same and/or different embodiments described. Thus, if one embodiment includes the feature A, B, C and another embodiment includes the feature B, D, then this application should also be considered to include embodiments that include one or more of all other possible combinations of A, B, C, D, although such embodiments may not be explicitly recited in text below.
The following description provides examples, and does not limit the scope, applicability, or examples set forth in the claims. Changes may be made in the function and arrangement of elements described without departing from the scope of the disclosure. Various examples may omit, substitute, or add various procedures or components as appropriate. For example, the described methods may be performed in an order different than the order described, and various steps may be added, omitted, or combined. Furthermore, features described with respect to some examples may be combined into other examples.
At present, in the field of substance detection, the application of a Raman spectrometer is wide, such as drugs, flammable and explosive dangerous chemicals, medicines and the like, and the detection only needs several seconds or dozens of seconds to obtain the result. The Raman spectrometer is mainly suitable for optical aspects such as physical and chemical laboratories, biological and medical fields and the like of scientific research institutions and higher institutions and is used for judging and confirming the components of research substances. And collecting the Raman spectrum scattered by the substance, and comparing the spectrum after pretreatment with the predicted substance spectrum to determine the name of the substance. The application is just applying the Raman spectrum to the field of clinical medicine, obtaining skin thickness data by using the Raman spectrum, and combining the skin thickness data with the Raman intensity data to accurately identify the recovery condition of the wound.
The human skin comprises two layers in the normal state: the epidermis layer is positioned above the dermis layer. Wounds on human skin usually involve the epidermis layer and the dermis layer, and the epidermis layer is usually damaged, and in order to accurately detect recovery from the wound, the skin thickness of the epidermis layer needs to be determined.
The raman spectrum scattered from the skin contains a number of signal peaks, some of which originate from the epidermis layer, some of which originate from the dermis layer, or a superposition of both signals. The raman signal peaks vary greatly with the thickness of the skin, corresponding to certain wavenumbers, as the raman signal comes from molecules in the epidermal layer. They therefore reflect a characteristic of the thickness of the epidermis layer and are therefore referred to as signal peaks. In the following description, the raman intensity corresponding to a signal peak is referred to as a first intensity. For some other wavenumbers, the raman signal peak is not sensitive to skin thickness because it comes from molecules in the dermis layer, and these raman signal peaks are called reference peaks. The raman intensity corresponding to the reference peak is referred to as a second intensity in the following description.
The epidermis does not contain tissues such as blood vessels, and the epidermis is relatively uniform. Suppose S Raman spectrum Raman intensity scattered into the epidermal layer, I Laser beam The laser intensity emitted by the laser emitting device, the raman intensity at the depth ζ of the epidermal layer is:
dS’ raman spectrum ＝aI’ Laser (1)
a is a coefficient, I ', proportional to Raman activity' Laser beam Is the intensity of the laser at the depth ζ. Both raman and laser light are attenuated through the epidermis layer, and therefore, the following relationships exist:
substituting (2) and (3) into (1),
the thickness of the epidermis is Z, the integral of the thickness of the whole epidermis is carried out to obtain the Raman intensity of the skin scattered back,
due to S Raman spectrum Not entirely from the epidermis layer, in the right equationAnd adding a bias constant to the side. Thus, the final first prediction model satisfies the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the first data.
The inventors have determined the first set of constants and performed a large number of raman spectroscopic measurements of different body parts of a plurality of subjects with one reflective raman system. Referring to fig. 1, fig. 1 is a schematic flow chart of determining a first set of constants in a first prediction model according to an embodiment of the present invention, where the method includes:
s101, collecting a plurality of ratios of different body parts of at least one subject.
Laser light in multiple wavenumber ranges can be transmitted by a raman spectroscopy instrument to multiple body parts of multiple subjects and corresponding raman spectra scattered back can be collected.
After numerous experiments, the inventors determined that the predetermined wave number in the above-mentioned Raman spectrum is from a spectrum range of 1110cm -1 ～1368cm -1 、1530cm -1 ～1850cm -1 Selected from within.
And S102, measuring the skin thickness of each different body part.
The skin thickness of each part of each subject is determined by a reference method such as Optical Coherence Tomography (OCT), ultrasound imaging, terahertz imaging, and near-infrared absorption spectroscopy.
And S103, generating a calibration curve of the correlation between the ratio and the skin thickness according to the ratio and the skin thickness obtained by each different body part.
Using the collected raman spectra, the ratio of all raman intensities was calculated. For each body part of all subjects, a ratio-skin thickness map was generated. For each body part, a cluster of data points is determined on the ratio-skin thickness map. For each cluster of data, a cluster center point is calculated. The candidate wave number pairs whose cluster center points satisfy a common functional relationship are retained, and the candidate wave number pair whose corresponding cluster center point expresses the common functional relationship in the most compact manner is selected as the optimal pair. In one embodiment, the candidate wavenumber pair that yields the smallest set standard deviation is selected as the best pair, and the predetermined wavenumber range in the raman spectrum is determined from the best pair.
And S104, determining the first group of constants by the calibration curve.
A correction curve based on a first prediction model is fitted, with a first set of constants R EP λ and R 0 Extracted by a least squares curve fit.
The following describes how the skin thickness is determined using the first predictive model described above. Fig. 2 is a schematic flow chart of a method for measuring the thickness of the epidermal skin according to an embodiment of the present invention, wherein the method comprises:
s201, receiving a second Raman signal from the skin of an intact part of the detected person, wherein the skin of the intact part is close to the skin of the part to be detected.
Because the skin of the part to be measured is the skin where the wound is located, the epidermis layer is usually in a damaged state, and the skin thickness of the epidermis layer cannot be accurately measured by directly testing the skin of the part to be measured. Therefore, skin of an intact part adjacent to the skin of the site to be measured is collected for detection.
S202, measuring a first intensity of the second Raman signal at a predetermined wavenumber and a second intensity of the Raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermis layer of the skin of the intact site, the second intensity being related to a thickness of a dermis layer of the skin of the intact site.
The predetermined wavenumber that can be in the second Raman spectrum is from a spectral range of 1110cm -1 ～1368cm -1 、 1530cm -1 ～1850cm -1 The selected first intensity and the selected second intensity.
S203, calculating the ratio of the first intensity and the second intensity.
And S204, deriving the first data according to the ratio and a first prediction model.
First data representing the thickness of the epidermis layer can be derived from the ratio and a first predictive model. In order to measure the skin thickness of the epidermis layer more accurately, raman spectra of normal skin at a plurality of sites adjacent to the wound of the same examiner are collected to determine the skin thicknesses of the plurality of adjacent sites, and the skin thickness of the wound site is determined based on the skin thicknesses of the plurality of adjacent sites.
The following describes how the skin thickness of the epidermal layer can be used to determine wound healing. Fig. 3 is a schematic flow chart of a method for measuring a wound of a subject according to an embodiment of the present invention, the method including:
s301, first data are obtained, and the first data are related to the thickness of the epidermis layer of the skin of the part to be detected of the detected person.
The specific implementation process of S301 may refer to the description of S201-S204 in fig. 2, and is not described herein again.
S302, receiving a first Raman signal from the skin of the part to be detected of the detected person.
And S303, obtaining second data according to the first Raman signal, wherein the second data is related to the content of the repair components in the dermis layer of the skin of the part to be detected.
A raman spectrum is typically composed of a certain number of raman peaks, each representing the wavelength position and intensity of the corresponding raman scattered light. Each raman peak corresponds to a particular molecular bond vibration. Raman spectroscopy is very sensitive to molecular bonding and the structure of the sample, so each molecule or sample has its own spectral "fingerprint". The content of the repair component is related to the repair degree of the wound, and the repair component can comprise: collagen, lipids, elastin, and sugars, among others. Molecules of different repair components in the dermis layer can correspond to a specific Raman peak, and the content of the repair components in the dermis layer of the skin of the part to be detected can be determined through Raman intensity of a Raman spectrum at a specific wavelength position.
S304, determining the skin wound characteristics of the part to be detected of the detected person according to the first data and the second data.
The first data represents the thickness of the epidermis layer at the site to be measured. The second data represents the content of the repair component in the dermis layer of the site to be measured. The repair composition may include a variety of substances. For example, the repair component may be collagen. Collagen is the main component of human connective tissue, affecting the healing of the skin at the wound site. Therefore, according to the first data and the second data, the skin wound characteristics of the part to be measured of the detected person can be determined.
As an example, wound healing can be classified into the following four cases from good to severe: the healing is good, common and poor. The wound healing conditions are not limited to the four conditions, and the user can set the healing conditions according to actual conditions, and the invention is not limited in any way.
The skin wound characteristics of the site to be tested of the subject can be determined by table 1 below. In table 1, the type and content of the repair component in the skin to be tested can be determined from the wavelength range corresponding to the second data and the raman intensity corresponding to the second data, and the degree of healing of the wound can be determined.
TABLE 1
It should be noted that the technical solution of the present invention is not limited to the above-mentioned manner of determining the characteristics of the skin wound through the list. All ways of determining the skin wound characteristics of the portion of the subject to be tested from the first data and the second data are within the scope of the present application.
The method for measuring the wound of the detected person can quickly identify the wound repair reaction through the Raman spectrum, and prompt doctors to understand and judge the wound reaction more scientifically, so that the method provides help for further medical treatment and medicine selection. Therefore, the invention can solve the problem of slow visual ability and pathology of the operating doctor in the prior art. The technical scheme of the invention is non-invasive, and the invention can detect wound without wound, thereby relieving the pain of the patient.
Another method for determining wound healing using the thickness of the epidermal layer is described below. Fig. 4 is a schematic flow chart of another method for measuring a wound of a subject according to an embodiment of the present invention, the method including:
s401, first data are obtained, and the first data are related to the thickness of the epidermis layer of the skin of the portion to be detected of the detected person.
The specific implementation process of S401 may refer to the description of S301 in fig. 3, and is not described herein again.
S402, receiving a third Raman signal from the skin of the part to be measured of the detected person at the first time.
The first time is an early time of wound detection. The specific implementation process of S402 may refer to the description of S302 in fig. 3, and is not described herein again.
And S403, obtaining second data according to the third Raman signal, wherein the second data is related to the content of the repair component in the dermis layer of the skin of the part to be detected in the first time.
The content of the repairing component is related to the repairing degree of the wound, and the repairing component can comprise: collagen, lipids, elastin, and sugars, among others.
The specific implementation process of S403 may refer to the description of S303 in fig. 3, and is not described herein again.
S404, determining wound characteristics of the examinee at a first time according to the first data and the second data.
The specific implementation process of S404 may refer to the description of S304 in fig. 3, and is not described herein again.
S405, receiving a fourth Raman signal from the skin of the part to be detected of the detected object at a second time.
The second time is a later time for wound detection. The specific implementation process of S405 may refer to the description of S301 in fig. 3, and is not described herein again.
And S406, obtaining third data according to the fourth Raman signal, wherein the third data is related to the content of the repair component in the dermis layer of the skin of the part to be detected at a second time.
The content of the repairing component is related to the repairing degree of the wound, and the repairing component can comprise: collagen, lipids, elastin, and sugars, among others.
The specific implementation process of S406 may refer to the description of S302 in fig. 3, and is not described herein again.
And S407, determining wound characteristics of the examinee at a second time according to the first data and the third data.
The specific implementation process of S407 may refer to the description of S303 in fig. 3, and is not described herein again.
S408, determining wound change characteristics of the subject according to the wound characteristics at the first time and the wound characteristics at the second time.
The invention relates to a method for detecting characteristic peak position change and intensity difference expression of wounds at different time points of tissues in chronic wounds and at wound edge parts by adopting a spectrum technology, determining the condition of the wounds, predicting the future recovery condition of the wounds and providing a basis for doctors and patients to establish correct treatment schemes. Specifically, if the wound recovery at the first time is worse than the wound recovery at the second time, the subject may be determined to have a better wound recovery trend. If the wound recovery at the first time and the wound recovery at the second time are both complete, it can be determined that the wound of the subject is not recovering and the patient can be advised to continue care and take medications. If the wound recovery at the first time is better than the wound recovery at the second time, a determination can be made that the subject's wound is worsening and the patient can be advised of treatment and debridement is necessary.
Embodiments of the present invention provide a method of determining the prognosis of a given wound that is relatively simple to perform, performs efficiently and provides an accurate indication of the likely outcome of the wound prior to or during treatment. The method uses a labeled sample with a high degree of representativeness to distinguish between acute wounds, chronic wounds and non-healing wounds, and is therefore particularly suitable for treatment options for a given wound.
The invention has obvious advantages clinically: the system has the advantages of quick identification and precision of dressing change, no need of sample collection, no wound, no pain, quick identification in operation, no waste of waiting time, reduction of the anesthesia dosage of a patient and the like, continuous spectrum information, better data support for decision making and the like.
Fig. 1 to 4 above illustrate in detail a method for measuring a wound of a subject according to an embodiment of the present application. Referring to fig. 5, fig. 5 is a schematic structural diagram of an apparatus for measuring a wound of a subject according to an embodiment of the present invention, and as shown in fig. 5, the apparatus for measuring a wound of a subject includes:
a first obtaining unit 501 for obtaining first data relating to a thickness of an epidermal layer of skin of a part to be measured of a subject;
a receiving unit 502 for receiving a first raman signal from the skin of the portion to be measured of the subject;
a second obtaining unit 503, configured to obtain second data according to the first raman signal, where the second data is related to a content of a repair component in a dermis layer of the skin of the site to be detected;
a determining unit 504, configured to determine a skin wound characteristic of the to-be-tested part of the subject according to the first data and the second data.
Optionally, the first obtaining unit 501 is specifically configured to:
receiving a second raman signal from skin of an intact part of the subject, the intact part of the skin being proximal to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermis layer of the skin of the intact site, the second intensity being related to a thickness of a dermis layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model.
Optionally, the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～ 1850cm -1 Selected from within.
Optionally, the first prediction model satisfies the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the first data.
Optionally, determining that the apparatus further comprises:
a constant determination unit 505 for collecting a plurality of said ratios of different body parts of at least one subject;
measuring the skin thickness of each of the different body parts;
generating a calibration curve relating ratio to skin thickness from said ratio and resulting skin thickness for each of said different body parts;
determining the first set of constants from the calibration curve.
Referring to fig. 6, fig. 6 is a schematic structural diagram of another apparatus for measuring a wound of a subject according to an embodiment of the present invention, and as shown in fig. 6, the apparatus for measuring a wound of a subject includes:
a first obtaining unit 601 for obtaining first data relating to a thickness of an epidermis layer of skin of a part to be measured of a subject;
a receiving unit 602 configured to receive a third raman signal from the skin of the portion to be measured of the subject at a first time;
a second obtaining unit 603 configured to obtain second data based on the third raman signal, the second data being related to a content of a repair component in a dermis layer of the skin of the site to be measured at a first time;
a determining unit 604 for determining a wound characteristic of the subject at a first time based on the first data and the second data;
the receiving unit 602 is further configured to receive a fourth raman signal from the skin of the portion to be measured of the subject at a second time;
the second obtaining unit 603 is further configured to obtain third data according to the fourth raman signal, where the third data is related to the content of the repair component in the dermis layer of the skin of the site to be measured at a second time;
the determining unit 604, further configured to determine a wound characteristic of the subject at a second time according to the first data and the third data;
a second determination unit 605 for determining a wound change characteristic of the subject according to the wound characteristic at the first time and the wound characteristic at the second time.
Optionally, the first obtaining unit 601 is specifically configured to:
receiving a fifth raman signal from skin of an intact part of the subject, the intact part of the skin being proximal to the skin of the site to be measured;
measuring a first intensity of the fifth raman signal at a predetermined wavenumber and a second intensity of the fifth raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermis layer of the skin of the intact site, the second intensity being related to a thickness of a dermis layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model.
Optionally, the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～ 1850cm -1 Selected from within.
Optionally, the first prediction model satisfies the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the first data or the third data.
Optionally, the apparatus further comprises:
a constant determination unit 606 for collecting a plurality of said ratios of different body parts of at least one subject;
measuring the skin thickness of each of the different body parts;
generating a calibration curve relating ratio to skin thickness from said ratio and skin thickness obtained for each of said different body parts;
determining the first set of constants from the calibration curve.
It is clear to a person skilled in the art that the solution according to the embodiments of the invention can be implemented by means of software and/or hardware. The "unit" and "module" in the present specification refer to software and/or hardware capable of performing a specific function independently or in cooperation with other components, wherein the hardware may be, for example, an FPGA (Field-Programmable Gate Array), an IC (Integrated Circuit), or the like.
Each processing unit and/or module according to the embodiments of the present invention may be implemented by an analog circuit that implements the functions described in the embodiments of the present invention, or may be implemented by software that executes the functions described in the embodiments of the present invention.
The embodiment of the invention also provides a system structure schematic diagram for measuring the wound of the detected person. A detection system, comprising: the device comprises a laser, a probe, a spectrum analyzer and a transmission optical fiber;
the laser transmits laser to the probe through the transmission optical fiber, so that the probe enables the laser to irradiate the skin of the predetermined position of the detected object to excite a Raman signal;
the probe collects the Raman signal and transmits the Raman signal to the spectrum analyzer through the transmission optical fiber;
and the spectrum analyzer performs spectrum analysis on the Raman signal by the method for measuring the wound of the examinee to obtain the skin wound characteristics of the part to be measured of the examinee.
Embodiments of the present invention also provide a computer readable storage medium having stored thereon a computer program which, when executed by a processor, performs the steps of the above method of measuring a wound of a subject. The computer-readable storage medium may include, but is not limited to, any type of disk including floppy disks, optical disks, DVDs, CD-ROMs, microdrive, and magneto-optical disks, ROMs, RAMs, EPROMs, EEPROMs, DRAMs, VRAMs, flash memory devices, magnetic or optical cards, nanosystems (including molecular memory ICs), or any type of media or device suitable for storing instructions and/or data.
Referring to fig. 7, a schematic structural diagram of an electronic device according to an embodiment of the present invention is shown, which can be used to implement the method for measuring a wound of a subject in the above embodiment. Specifically, the method comprises the following steps: the electronic device 1000 may include: at least one processor 1001, at least one network interface 1004, a user interface 1003, memory 1005, at least one communication bus 1002.
Wherein a communication bus 1002 is used to enable connective communication between these components.
The user interface 1003 may include a Display screen (Display) and a Camera (Camera), and the optional user interface 1003 may also include a standard wired interface and a wireless interface.
The network interface 1004 may optionally include a standard wired interface, a wireless interface (e.g., WI-FI interface), among others.
The Memory 1005 may include a Random Access Memory (RAM) or a Read-Only Memory (Read-Only Memory). Optionally, the memory 1005 includes a non-transitory computer-readable medium. The memory 1005 may be used to store an instruction, a program, code, a set of codes, or a set of instructions. The memory 1005 may include a program storage area and a data storage area, wherein the program storage area may store instructions for implementing an operating system, instructions for at least one function (such as a touch function, a sound playing function, an image playing function, etc.), instructions for implementing the above-described method embodiments, and the like; the storage data area may store data and the like referred to in the above respective method embodiments. The memory 1005 may optionally be at least one memory device located remotely from the processor 1001. As shown in fig. 7, a memory 1005, which is a kind of computer storage medium, may include therein an operating system, a network communication module, a user interface module, and a bluetooth pairing application program.
In the several embodiments provided in the present application, it should be understood that the disclosed apparatus and method may be implemented in other manners. The above-described device embodiments are merely illustrative, for example, the division of the unit is only a logical functional division, and there may be other division ways in actual implementation, such as: multiple units or components may be combined, or may be integrated into another system, or certain features may be omitted, or not implemented. In addition, the coupling, direct coupling or communication connection between the components shown or discussed may be through some interfaces, and the indirect coupling or communication connection between the devices or units may be electrical, mechanical or other forms.
All functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may be separately used as one unit, or two or more units may be integrated into one unit; the integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional unit.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A device for measuring a wound of a subject, the device comprising:
a first obtaining unit configured to obtain first data relating to a thickness of an epidermal layer of skin of a part to be measured of a subject;
a receiving unit configured to receive a first raman signal from skin of the portion to be measured of the subject;
a second obtaining unit configured to obtain second data based on the first raman signal, the second data being related to a content of a repair component in a dermis layer of the skin of the site to be measured, the repair component including collagen;
a determination unit for determining the skin wound characteristics of the part to be measured of the examinee according to the first data and the second data,
the determining the skin wound characteristics of the part to be measured of the subject according to the first data and the second data comprises the following steps:
determining the skin wound characteristics of the part to be measured of the examinee according to the corresponding relation between the first data and the wound characteristics and the second data, wherein the second data comprises wavelength positions and Raman intensities,
the obtaining first data comprises:
receiving a second Raman signal from skin of an intact part of the subject, the intact part of the skin being adjacent to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model,
the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～1850cm -1 Selected from within.
2. A device for measuring a wound of a subject, the device comprising:
a first obtaining unit configured to obtain first data relating to a thickness of an epidermal layer of skin of a part to be measured of a subject;
a receiving unit configured to receive a third raman signal from the skin of the portion to be measured of the subject at a first time;
a second obtaining unit configured to obtain second data based on the third raman signal, the second data being related to a content of a repair component in a dermis layer of the skin of the site to be measured at a first time;
a determination unit for determining a wound characteristic of the subject at a first time from the first data and the second data;
the receiving unit is further used for receiving a fourth Raman signal from the skin of the part to be measured of the detected object at a second time;
the second obtaining unit is further configured to obtain third data according to the fourth raman signal, where the third data is related to a content of a repair component in a dermal layer of the skin of the site to be measured at a second time, and the repair component includes collagen;
the determination unit is further configured to determine wound characteristics of the subject at a second time according to the first data and the third data;
a second determination unit for determining a wound change characteristic of the subject based on the wound characteristic at the first time and the wound characteristic at the second time,
the determining a wound characteristic of the subject at a first time from the first data and the second data comprises:
determining a wound characteristic of the subject at a first time based on the correspondence of the first data and second data to the wound characteristic, the second data comprising a wavelength location and a Raman intensity,
the obtaining first data comprises:
receiving a second raman signal from skin of an intact part of the subject, the intact part of the skin being proximal to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model,
the predetermined wavenumber isFrom the spectral range 1110cm -1 ～1368cm -1 、1530cm -1 ～1850cm -1 Selected from the list.
3. A detection system, comprising: the device comprises a laser, a probe, a spectrum analyzer and a transmission optical fiber;
the laser transmits laser to the probe through the transmission optical fiber, so that the probe enables the laser to irradiate the skin of the predetermined position of the detected object to excite a Raman signal;
the probe collects the Raman signal and transmits the Raman signal to the spectrum analyzer through the transmission optical fiber;
the spectrum analyzer is used for carrying out spectrum analysis on the Raman signal to obtain the skin wound characteristics of the part to be detected of the detected person,
the spectrum analyzer is configured to:
obtaining first data relating to a thickness of an epidermal layer of skin of a site to be examined of a subject;
receiving a first Raman signal from skin of the site to be measured of the subject;
obtaining second data according to the first Raman signal, wherein the second data is related to the content of a repair component in a dermis layer of the skin of the part to be detected, and the repair component comprises collagen;
determining the skin wound characteristics of the part to be measured of the examinee according to the corresponding relation between the first data and the wound characteristics and the second data, wherein the second data comprises wavelength positions and Raman intensities,
the obtaining first data comprises:
receiving a second Raman signal from skin of an intact part of the subject, the intact part of the skin being adjacent to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model,
the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～1850cm -1 Selected from within.
4. The detection system of claim 3, the first predictive model satisfying the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the first data.
5. The detection system of claim 4, the determining the first set of constants comprising:
collecting a plurality of said ratios of different body parts of at least one subject;
measuring the skin thickness of each of the different body parts;
generating a calibration curve relating ratio to skin thickness from said ratio and resulting skin thickness for each of said different body parts;
determining the first set of constants from the calibration curve.
6. The detection system of claim 3, the spectrum analyzer configured to:
obtaining first data relating to a thickness of an epidermal layer of skin of a site to be examined of a subject;
receiving a third Raman signal from skin of the site to be measured of the subject at a first time;
obtaining second data according to the third Raman signal, wherein the second data is related to the content of the repair components in the dermis layer of the skin of the part to be detected at the first time;
determining, from the first data and the second data, wound characteristics of the subject at a first time;
receiving a fourth Raman signal from the skin of the site to be measured of the subject at a second time;
obtaining third data according to the fourth Raman signal, wherein the third data is related to the content of the repair component in the dermis layer of the skin of the part to be detected at a second time;
determining wound characteristics of the subject at a second time according to the first data and the third data;
determining a wound change characteristic of the subject from the wound characteristics at the first time and the wound characteristics at the second time.
7. The detection system of claim 6, the obtaining first data comprising:
receiving a fifth Raman signal from skin of an intact part of the subject, the intact part of the skin being adjacent to the skin of the site to be measured;
measuring a first intensity of the fifth raman signal at a predetermined wavenumber and a second intensity of the fifth raman signal at a predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model.
8. The detection system of claim 7, the first predictive model satisfying the following equation:
wherein R is the ratio of the first intensity and the second intensity, R EP λ and R 0 Is a first set of constants and Z is the third data.
9. A computer-readable storage medium on which a computer program is stored, the program, when executed by a processor, performing the steps of:
obtaining first data relating to a thickness of an epidermal layer of skin of a site to be examined of a subject;
receiving a first Raman signal from skin of the site to be measured of the subject;
obtaining second data from the first raman signal, the second data relating to a content of a repair component in a dermal layer of the skin of the site to be tested, the repair component comprising collagen;
determining skin wound characteristics of the part to be detected of the detected person according to the corresponding relation between the first data and the wound characteristics and the second data, wherein the second data comprises wavelength positions and Raman intensities,
the obtaining first data comprises:
receiving a second Raman signal from skin of an intact part of the subject, the intact part of the skin being adjacent to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model,
the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～1850cm -1 Selected from within.
10. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, wherein the processor when executing the program performs the steps of:
obtaining first data relating to a thickness of an epidermal layer of skin of a site to be examined of a subject;
receiving a first Raman signal from skin of the site to be measured of the subject;
obtaining second data from the first raman signal, the second data relating to a content of a repair component in a dermal layer of the skin of the site to be tested, the repair component comprising collagen;
determining skin wound characteristics of the part to be detected of the detected person according to the corresponding relation between the first data and the wound characteristics and the second data, wherein the second data comprises wavelength positions and Raman intensities,
the obtaining first data comprises:
receiving a second raman signal from skin of an intact part of the subject, the intact part of the skin being proximal to the skin of the site to be measured;
measuring a first intensity of the second raman signal at a predetermined wavenumber and a second intensity of the raman signal at the predetermined wavenumber, the first intensity being related to a thickness of an epidermal layer of the skin of the intact site, the second intensity being related to a thickness of a dermal layer of the skin of the intact site;
calculating a ratio of the first intensity and the second intensity;
deriving the first data from the ratio and a first predictive model,
the predetermined wavenumber is 1110cm from the spectral range -1 ～1368cm -1 、1530cm -1 ～1850cm -1 Selected from the list.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911238458.XA CN112914503B (en) | 2019-12-06 | 2019-12-06 | Method, apparatus, storage medium, and electronic device for measuring wound of subject |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911238458.XA CN112914503B (en) | 2019-12-06 | 2019-12-06 | Method, apparatus, storage medium, and electronic device for measuring wound of subject |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112914503A CN112914503A (en) | 2021-06-08 |
| CN112914503B true CN112914503B (en) | 2023-03-28 |
Family
ID=76161325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911238458.XA Active CN112914503B (en) | 2019-12-06 | 2019-12-06 | Method, apparatus, storage medium, and electronic device for measuring wound of subject |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112914503B (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9924894B2 (en) * | 2015-06-03 | 2018-03-27 | Hong Kong Applied Science And Technology Research Institute Co. Ltd. | Non-invasive measurement of skin thickness and glucose concentration with Raman spectroscopy and method of calibration thereof |
-
2019
- 2019-12-06 CN CN201911238458.XA patent/CN112914503B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112914503A (en) | 2021-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20110208084A1 (en) | Method and apparatus for brain damage detection | |
| JP2003527916A (en) | Method and apparatus for diagnostic multispectral digital imaging | |
| EP2661615B1 (en) | An apparatus for optical analysis of an associated tissue | |
| CN109781701A (en) | Real-time detection method in a kind of parathyroidectomy based on Raman spectroscopy | |
| Regensburger et al. | Multispectral optoacoustic tomography for non-invasive disease phenotyping in pediatric spinal muscular atrophy patients | |
| JP2007524833A (en) | Method and apparatus for diagnosing bone tissue condition | |
| Negi et al. | Role of near-infrared spectroscopy in intravascular coronary imaging | |
| Abraham et al. | Comparison of transcutaneous oximetry with symptoms and arteriography in thoracic outlet syndrome | |
| Wang et al. | EEG-derived pain threshold index for prediction of postoperative pain in patients undergoing laparoscopic urological surgery: a comparison with surgical pleth index | |
| Li et al. | Multispectral raster-scanning optoacoustic mesoscopy differentiate lesional from non-lesional atopic dermatitis skin using structural and functional imaging markers | |
| CN112951426B (en) | Construction method and evaluation system of pancreatic duct adenoma inflammatory infiltration degree judgment model | |
| CN112914503B (en) | Method, apparatus, storage medium, and electronic device for measuring wound of subject | |
| US20140321728A1 (en) | Methods for detection and characterization of ionizing radiation exposure in tissue | |
| Bi et al. | A portable optical pulsatile flowmetry demonstrates strong clinical relevance for diabetic foot perfusion assessment | |
| RU183278U1 (en) | DEVICE FOR NEUROSURGICAL ASPIRATION WITH SPECTROSCOPIC AND ELECTROPHYSIOLOGICAL CONTROL | |
| CN114938974A (en) | Method and system for predicting pancreatic fistula risk based on ultrasonic elasticity technology | |
| Promny et al. | Evaluation of hyperspectral imaging as a modern aid in clinical assessment of burn wounds of the upper extremity | |
| US11382525B2 (en) | Handheld blood-flow imaging device | |
| EP2642910B1 (en) | Device and method for determining a disease activity | |
| KR102290280B1 (en) | Non-invasive measuring device and method for bio-analyte and diagnosing device and method for diagnosing metabolic syndrome | |
| Queen et al. | Importance of imaging to wound care practice | |
| RU2758563C1 (en) | Method for diagnosis of skin melanoma | |
| CN111539932B (en) | Hemoglobin measuring instrument and method | |
| RU2204945C2 (en) | Method for giving differential evaluation of lower extremity muscular tissue | |
| RU2247379C1 (en) | Differential diagnosis method for detecting destructive changes in the cases of various forms of acute cholecystitis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |