CN112903542B - Microsphere open type piezoelectric micro-control measurement system and method based on standing wave sound field - Google Patents

Microsphere open type piezoelectric micro-control measurement system and method based on standing wave sound field Download PDF

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CN112903542B
CN112903542B CN202110063178.0A CN202110063178A CN112903542B CN 112903542 B CN112903542 B CN 112903542B CN 202110063178 A CN202110063178 A CN 202110063178A CN 112903542 B CN112903542 B CN 112903542B
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micro
container
piezoelectric ceramic
microsphere
cylinder
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CN112903542A (en
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冯浩人
王亮
王鑫
金家楣
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Nanjing University of Aeronautics and Astronautics
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Nanjing University of Aeronautics and Astronautics
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0038Investigating nanoparticles

Abstract

The invention discloses a microsphere open type piezoelectric micro-manipulation measuring system and method based on a standing wave sound field, wherein the measuring system comprises a micro-manipulation device, a micro-detection device and a calculating device; the micro control device comprises a container, a pre-tightening module, a piezoelectric ceramic module and a fixed cylinder; one end of the pre-tightening module is connected with the fixed cylinder, the other end of the pre-tightening module is connected with the container, and the piezoelectric ceramic module is clamped in the pre-tightening module; the microscopic detection device adopts a commercial industrial microscope, and the lower end of the fixed cylinder is placed on an object stage of the microscopic detection device, so that the container is horizontally placed. When the micro-detection device works, the micro-detection device observes the image of the microsphere in the container and transmits the image to the computing device; and the computing device analyzes the received images and obtains the motion trail of the microspheres and the morphological parameters thereof through machine learning. The invention has simple equipment and low price, realizes the nondestructive and contactless control of the microsphere by adopting the piezoelectric excitation micro control device, and can quickly obtain the motion trail and the morphological parameters of the microsphere.

Description

Microsphere open type piezoelectric micro-control measurement system and method based on standing wave sound field
Technical Field
The invention relates to the field of micro-manipulation and micro-particle rapid screening, in particular to a microsphere open type piezoelectric micro-manipulation measuring system and method based on a standing wave sound field.
Background
The laser confinement nuclear fusion ICF takes high-power and high-energy density laser as a driving source, and adopts a spherical implosion pressurization technology to enable nuclear fuel in a spherical target pellet to reach an ignition condition, so that a self-sustained thermonuclear reaction is formed. ICF is expected to provide clean and pollution-free energy for human beings. The ICF experiment has strict requirements on the quality of hollow microspheres (target pellets) serving as nuclear fuel containers in the aspects of geometric parameters, surface defects and the like, and the quality of the target pellets directly influences the success or failure of the ICF targeting experiment. At present, the detection devices used for measuring the geometric parameters of the microspheres include an X-ray apparatus, a white light interferometer, an atomic force microscope, and the like. The measurement precision of the instruments is high (can reach micron-scale or even nanometer-scale). However, the detection efficiency of these devices is very low, and all adopt a manual detection mode, and the subjective factor influence of microsphere screening is large. Moreover, because the preparation amount of the target pellets is huge and the detection efficiency of the existing equipment is low, a high-precision screening method cannot be adopted completely, so that a device for quickly measuring and screening the microspheres is urgently needed, and the screening efficiency of the microspheres is improved.
As the target pill has the characteristics of small size (diameter of 100-1000 mu m), fragile structure, strong viscosity and the like, the equipment for controlling the microspheres cannot cause secondary damage to the microspheres. The micro-manipulation technology using the sound wave as the driving source has the advantages of high biocompatibility, stable micro-scale manipulation and the like, and means that the micro-manipulation technology can be applied to nondestructive detection and measurement of the microspheres.
Disclosure of Invention
The invention aims to solve the technical problem of providing a microsphere open type piezoelectric micro-manipulation measuring system and method based on a standing wave sound field aiming at the defects involved in the background technology.
The invention adopts the following technical scheme for solving the technical problems:
the microsphere open type piezoelectric micro-manipulation measuring system based on the standing wave sound field comprises a micro-manipulation device, a micro-detection device and a calculating device;
the micro-control device comprises a container, a pre-tightening module, a piezoelectric ceramic module and a fixed cylinder;
the container is a hollow cylinder with an opening at the upper end and a closed lower end;
the piezoelectric ceramic module comprises 2n annular piezoelectric ceramic pieces, wherein n is a natural number more than or equal to 1;
the pre-tightening module comprises a connecting circular truncated cone, a connecting column, a connecting cylinder and a pre-tightening bolt, wherein the pre-tightening bolt comprises a nut and a stud; the connecting column is cylindrical, and a threaded hole matched with the pre-tightening bolt is formed in the lower end face of the connecting column along the axis; the connecting circular truncated cone is in a circular truncated cone shape, one end with a smaller area is coaxially and fixedly connected with the lower end face of the container, and the other end with a larger area is coaxially and fixedly connected with the upper end face of the connecting column; the connecting cylinder is a hollow cylinder with an opening at the lower end and a closed upper end, and a through hole for the stud of the pre-tightening bolt to pass through is formed in the center of the upper end face of the connecting cylinder; the nut of the pre-tightening bolt is positioned in the connecting cylinder, and the stud sequentially penetrates through the through hole in the upper end face of the connecting cylinder and the through hole in the center of the 2n piezoelectric ceramic pieces and then is in threaded connection with the threaded through hole of the connecting column, so that the 2n piezoelectric ceramic pieces are clamped between the connecting column and the connecting cylinder, and pre-pressures at two ends of the 2n piezoelectric ceramic pieces are adjusted;
the 2n piezoelectric ceramic pieces are polarized along the thickness direction of the piezoelectric ceramic pieces, and the polarization directions of the adjacent piezoelectric ceramic pieces are opposite;
two sides of the side wall of the connecting cylinder are provided with symmetrical connecting lugs;
the fixed cylinder is a through hole cylinder with openings at two ends, is sleeved outside the connecting cylinder, and the upper end of the fixed cylinder is fixedly connected with the connecting lugs at two sides of the connecting cylinder;
the container is used for containing a liquid bearing medium and microspheres to be measured for morphological parameters in the liquid bearing medium;
the connecting cylinder, the piezoelectric ceramic module, the connecting column and the connecting circular truncated cone form an energy converter, the piezoelectric ceramic module is used for applying a simple harmonic voltage signal to excite m-order longitudinal vibration of the energy converter so as to induce Bm + 2-order bending vibration of the container, and m is an odd number which is more than or equal to 1;
the micro-detection device adopts a commercial industrial microscope, and the lower end of the micro-control device fixing cylinder is placed on an object stage of the micro-detection device, so that the container is horizontally placed; the microscopic detection device is used for observing the image of the microsphere in the container and transmitting the image to the computing device;
the computing device is used for receiving and analyzing the images acquired by the microscopic detection device, and obtaining the movement track of the microspheres and the morphological parameters thereof through machine learning.
As a further optimization scheme of the microsphere open type piezoelectric micro-control measurement system based on the standing wave sound field, a pair of flat keys are arranged on the side wall of the connecting column to be matched with the pre-tightening bolts to adjust pre-pressures at two ends of 2n piezoelectric ceramic pieces.
As a further optimization scheme of the microsphere open type piezoelectric micro-manipulation measuring system based on the standing wave sound field, n is 2.
The invention also discloses a micro-control method of the microsphere open type piezoelectric micro-control measurement system based on the standing wave sound field, which comprises the following steps:
applying a simple harmonic voltage signal with the frequency of a preset first frequency threshold f1 to the piezoelectric ceramic module, exciting a first-order longitudinal vibration mode of the transducer, and inducing a B30 bending vibration mode of the container, wherein the sound field distribution in the liquid bearing medium is consistent with the mode, namely, the sound pressure node corresponds to a mode node, the sound pressure antinode corresponds to the maximum mode amplitude, and the microspheres placed in the liquid bearing medium of the container move towards the sound pressure node under the action of sound radiation force and liquid viscous force; the microspheres arranged near the circle center of the container are gathered at the circle center and do autorotation motion under the action of the acoustic flow;
and stopping applying the simple harmonic voltage signal with the frequency f1, applying the simple harmonic voltage signal with the frequency of a preset second frequency threshold value f2 to the piezoelectric ceramic module, exciting a third-order longitudinal vibration mode of the transducer, and inducing a B50 bending vibration mode of the container, wherein the microspheres gathered at the center of the circle of the container move outwards and stop on the pitch circle closest to the center of the circle due to the difference in distribution of pitch circle spaces of the B30 bending vibration mode and the B50 bending vibration mode.
Compared with the prior art, the invention adopting the technical scheme has the following technical effects:
1. the equipment is simple, the price is low, and the cost of a common measurement system can be reduced;
2. the piezoelectric-excited micro-control device can realize the nondestructive and contactless control of the microspheres, and the control means comprises: positioning control, rotation control and migration control;
3. the computing device can rapidly obtain the movement track of the microsphere and the morphological parameters thereof through machine learning.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural diagram of the micromanipulation device of the present invention;
FIG. 3 is a schematic view of the structure of the container, the connecting round table and the connecting column of the present invention;
FIG. 4 is a schematic view of the construction of the microscopic examination apparatus according to the present invention;
FIG. 5 is a schematic diagram of a simulation of the tank and transducer B30 mode of the present invention;
FIG. 6 is a schematic diagram of the nodal line distribution of acoustic pressure induced by the B30 mode in a container of the present invention;
FIG. 7 is a schematic representation of the sound pressure distribution and microsphere motion on the diameter line of a container induced by the B30 mode in the container of the present invention;
FIG. 8 is a schematic diagram of a simulation of the tank and transducer B50 mode of the present invention;
FIG. 9 is a schematic diagram of the nodal line distribution of acoustic pressure induced by the B50 mode in a container of the present invention;
fig. 10 is a graphical representation of the sound pressure distribution and microsphere movement on the diameter line of the container induced by the B50 mode in the container of the present invention.
In the figure, 1-a micro-control device, 1.1-a container, 1.2-a piezoelectric ceramic module, 1.3.1-a connecting round table, 1.3.2-a connecting column, 1.3.3-a connecting cylinder, 1.3.4-a pre-tightening bolt, 1.4-a fixed cylinder, 2-a micro-detection device, 2.1-a CCD camera, 2.2-a microscope lens, 2.3-a microscope bracket, 2.4-an object stage, 2.5-a display screen and 3-a calculation device.
Detailed Description
The technical scheme of the invention is further explained in detail by combining the attached drawings:
the present invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. In the drawings, components are exaggerated for clarity.
As shown in FIG. 1, the invention discloses a microsphere open type piezoelectric micro-manipulation measuring system based on a standing wave sound field, which is characterized by comprising a micro-manipulation device, a micro-detection device and a calculation device.
The micro-control device comprises a container, a pre-tightening module, a piezoelectric ceramic module and a fixed cylinder; the container is a hollow cylinder with an opening at the upper end and a closed lower end; as shown in fig. 2, the piezoelectric ceramic module includes 2n annular piezoelectric ceramic pieces, where n is a natural number greater than or equal to 1; the pre-tightening module comprises a connecting circular truncated cone, a connecting column, a connecting cylinder and a pre-tightening bolt, wherein the pre-tightening bolt comprises a nut and a stud; the connecting column is cylindrical, and a threaded through hole matched with the pre-tightening bolt is formed in the lower end face of the connecting column along the axis; the connecting circular truncated cone is in a circular truncated cone shape, one end with a smaller area is coaxially and fixedly connected with the lower end face of the container, and the other end with a larger area is coaxially and fixedly connected with the upper end face of the connecting column; the connecting cylinder is a hollow cylinder with an opening at the lower end and a closed upper end, and a through hole for the stud of the pre-tightening bolt to pass through is formed in the center of the upper end face of the connecting cylinder, as shown in fig. 3; the nut of the pre-tightening bolt is positioned in the connecting cylinder, and the stud sequentially penetrates through the through hole in the upper end face of the connecting cylinder and the through hole in the center of the 2n piezoelectric ceramic pieces and then is in threaded connection with the threaded through hole of the connecting column, so that the 2n piezoelectric ceramic pieces are clamped between the connecting column and the connecting cylinder, and pre-pressures at two ends of the 2n piezoelectric ceramic pieces are adjusted; the 2n piezoelectric ceramic pieces are polarized along the thickness direction of the piezoelectric ceramic pieces, and the polarization directions of the adjacent piezoelectric ceramic pieces are opposite; two sides of the side wall of the connecting cylinder are provided with symmetrical connecting lugs; the fixed cylinder is a through hole cylinder with openings at two ends, is sleeved outside the connecting cylinder, and the upper end of the fixed cylinder is fixedly connected with the connecting lugs at two sides of the connecting cylinder.
The container is used for containing a liquid bearing medium and microspheres to be measured for morphological parameters in the liquid bearing medium; the microspheres to be separated in the invention comprise microparticles, cells, organisms and the like, and the size of the microspheres is between nanometer and millimeter; according to the difference of the microspheres, different liquid carrying media such as deionized water, absolute ethyl alcohol, culture solution, tissue fluid and the like need to be added into the container.
The connecting cylinder, the piezoelectric ceramic module, the connecting column and the connecting circular truncated cone form an energy converter, the piezoelectric ceramic module is used for applying a simple harmonic voltage signal to excite m-order longitudinal vibration of the energy converter so as to induce Bm + 2-order bending vibration of the container, and m is an odd number greater than or equal to 1, as shown in fig. 5 and 6.
As shown in fig. 4, the microscopic detection device adopts a commercial industrial microscope, and comprises a CCD camera, a microscope lens, a microscope stand, an object stage and a display screen; the lower end of the micro-control device fixing cylinder is placed on an object stage of the micro-detection device, so that the container is horizontally placed; the microspheres in the container of the micro-control device are observed through the combination of the microscope lens and the CCD camera, and the observed microsphere images are displayed on the display screen in real time and transmitted to the computing device.
The computing device is used for receiving and analyzing the images acquired by the microscopic detection device, and obtaining the movement track of the microspheres and the morphological parameters thereof through machine learning.
The computing device can adopt a computer or a computing chip.
A pair of flat keys is arranged on the side wall of the connecting column to be matched with the pre-tightening bolt to adjust pre-pressure at two ends of the 2n piezoelectric ceramic pieces; n preferably takes 2.
The invention also discloses a micro-control method of the microsphere open type piezoelectric micro-control measurement system based on the standing wave sound field, which takes the case that n is 2, and comprises the following steps:
applying a simple harmonic voltage signal with a frequency of a preset first frequency threshold f1 to the piezoelectric ceramic module, exciting a first-order longitudinal vibration mode of the transducer, and inducing a B30 bending vibration mode of the container, as shown in FIG. 5, at this time, the sound field distribution in the liquid bearing medium is consistent with the mode, that is, the sound pressure node corresponds to a mode node, and the sound pressure antinode corresponds to the maximum mode amplitude, that is, the liquid bearing medium in the container has three sound pressure nodal circles, as shown in FIG. 6; the microspheres placed in the liquid carrying medium of the container will move towards the sound pressure node under the action of the acoustic radiation force and the liquid viscous force, as shown in fig. 7; the microspheres arranged near the circle center of the container are gathered at the circle center and do autorotation motion under the action of the acoustic flow;
stopping applying the simple harmonic voltage signal with the frequency f1, applying the simple harmonic voltage signal with the frequency f2 preset as a second frequency threshold to the piezoelectric ceramic module, exciting a third-order longitudinal vibration mode of the transducer, and inducing a B50 bending vibration mode of the container, as shown in FIG. 8; the liquid carrying medium in the container now has five sound pressure pitch circles, as shown in fig. 9; due to the difference in spatial distribution of the pitch circles of the B30 bending mode and the B50 bending mode, the microspheres collected at the center of the container move outward and stop at the pitch circle closest to the center of the container, as shown in fig. 10.
The micro-control method comprises three control means of positioning the center of a circle of the microsphere, rotating the center of the circle and performing directional motion.
It should be noted that the positioning and rotation of the microspheres are not limited to the center of the container, but can be implemented on each pitch circle, and the migration path of the microspheres is not limited to the center to the pitch circle, but also includes migration between pitch circles in multiple modes.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The above-mentioned embodiments, objects, technical solutions and advantages of the present invention are further described in detail, it should be understood that the above-mentioned embodiments are only illustrative of the present invention and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (4)

1. The microsphere open type piezoelectric micro-manipulation measuring system based on the standing wave sound field is characterized by comprising a micro-manipulation device, a micro-detection device and a calculating device;
the micro-control device comprises a container, a pre-tightening module, a piezoelectric ceramic module and a fixed cylinder;
the container is a hollow cylinder with an opening at the upper end and a closed lower end;
the piezoelectric ceramic module comprises 2n annular piezoelectric ceramic pieces, wherein n is a natural number more than or equal to 1;
the pre-tightening module comprises a connecting circular truncated cone, a connecting column, a connecting cylinder and a pre-tightening bolt, wherein the pre-tightening bolt comprises a nut and a stud; the connecting column is cylindrical, and a threaded hole matched with the pre-tightening bolt is formed in the lower end face of the connecting column along the axis; the connecting circular truncated cone is in a circular truncated cone shape, one end with a smaller area is coaxially and fixedly connected with the lower end face of the container, and the other end with a larger area is coaxially and fixedly connected with the upper end face of the connecting column; the connecting cylinder is a hollow cylinder with an opening at the lower end and a closed upper end, and a through hole for the stud of the pre-tightening bolt to pass through is formed in the center of the upper end face of the connecting cylinder; the nut of the pre-tightening bolt is positioned in the connecting cylinder, and the stud sequentially penetrates through the through hole in the upper end face of the connecting cylinder and the through hole in the center of the 2n piezoelectric ceramic pieces and then is in threaded connection with the threaded hole of the connecting column, so that the 2n piezoelectric ceramic pieces are clamped between the connecting column and the connecting cylinder, and pre-pressures at two ends of the 2n piezoelectric ceramic pieces are adjusted;
the 2n piezoelectric ceramic pieces are polarized along the thickness direction of the piezoelectric ceramic pieces, and the polarization directions of the adjacent piezoelectric ceramic pieces are opposite;
two sides of the side wall of the connecting cylinder are provided with symmetrical connecting lugs;
the fixed cylinder is a through hole cylinder with openings at two ends, is sleeved outside the connecting cylinder, and the upper end of the fixed cylinder is fixedly connected with the connecting lugs at two sides of the connecting cylinder;
the container is used for containing a liquid bearing medium and microspheres to be measured for morphological parameters in the liquid bearing medium;
the connecting cylinder, the piezoelectric ceramic module, the connecting column and the connecting circular truncated cone form an energy converter, the piezoelectric ceramic module is used for applying a simple harmonic voltage signal to excite m-order longitudinal vibration of the energy converter so as to induce Bm + 2-order bending vibration of the container, and m is an odd number which is more than or equal to 1;
the micro-detection device adopts a commercial industrial microscope, and the lower end of the micro-control device fixing cylinder is placed on an object stage of the micro-detection device, so that the container is horizontally placed; the microscopic detection device is used for observing the image of the microsphere in the container and transmitting the image to the computing device;
the computing device is used for receiving and analyzing the images acquired by the microscopic detection device, and obtaining the movement track of the microspheres and the morphological parameters thereof through machine learning.
2. The microsphere open type piezoelectric micro-manipulation measuring system based on the standing wave sound field according to claim 1, wherein a pair of flat keys are arranged on the side wall of the connecting column to match with the pre-tightening bolts to adjust pre-pressures at two ends of 2n piezoelectric ceramic sheets.
3. The standing wave acoustic field-based microsphere open piezoelectric micromanipulation measurement system of claim 1, wherein n is 2.
4. The micro-manipulation method of the microsphere open type piezoelectric micro-manipulation measuring system based on the standing wave sound field of claim 1, which is characterized by comprising the following steps:
applying a simple harmonic voltage signal with the frequency of a preset first frequency threshold f1 to the piezoelectric ceramic module, exciting a first-order longitudinal vibration mode of the transducer, and inducing a B30 bending vibration mode of the container, wherein the sound field distribution in the liquid bearing medium is consistent with the mode, namely, the sound pressure node corresponds to a mode node, the sound pressure antinode corresponds to the maximum mode amplitude, and the microspheres placed in the liquid bearing medium of the container move towards the sound pressure node under the action of sound radiation force and liquid viscous force; the microspheres arranged near the circle center of the container are gathered at the circle center and do autorotation motion under the action of the acoustic flow;
and stopping applying the simple harmonic voltage signal with the frequency f1, applying the simple harmonic voltage signal with the frequency of a preset second frequency threshold value f2 to the piezoelectric ceramic module, exciting a third-order longitudinal vibration mode of the transducer, and inducing a B50 bending vibration mode of the container, wherein the microspheres gathered at the center of the circle of the container move outwards and stop on the pitch circle closest to the center of the circle due to the difference in distribution of pitch circle spaces of the B30 bending vibration mode and the B50 bending vibration mode.
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