CN112899126A - Fermenting installation of bacillus subtilis microbial preparation - Google Patents

Fermenting installation of bacillus subtilis microbial preparation Download PDF

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CN112899126A
CN112899126A CN202110413279.6A CN202110413279A CN112899126A CN 112899126 A CN112899126 A CN 112899126A CN 202110413279 A CN202110413279 A CN 202110413279A CN 112899126 A CN112899126 A CN 112899126A
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wall
gear
disc
mixing tank
rotating
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CN112899126B (en
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蒋小丰
余锐
刘亚力
罗国升
潘长咏
戴富全
彭地纬
常翠维
龚龑
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Hunan Perfly Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers

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Abstract

The invention discloses a fermentation device of a bacillus subtilis microbial preparation, and relates to the field of bacillus subtilis production. According to the invention, by arranging the fermentation tank, the sealing cover, the mixing mechanism, the mixing tank, the spraying mechanism, the oxygen supply mechanism and the corrugated pipe, after fermentation is carried out for a fixed time, materials at the bottom end of the mixing tank begin to agglomerate, so that the fermentation of the materials in the mixing tank is not uniform, at the moment, the mixing mechanism is started again to transport the deposited and agglomerated materials at the bottom end of the mixing tank to the upper part of the mixing tank through the helical blades, and the materials in the mixing tank are stirred and mixed again, so that the uniformity of material fermentation is ensured, a user can conveniently and accurately control the whole fermentation progress of the materials in the fermentation tank, and the production quality of the.

Description

Fermenting installation of bacillus subtilis microbial preparation
Technical Field
The invention relates to the field of bacillus subtilis production, in particular to a fermentation device for a bacillus subtilis microbial preparation.
Background
With the gradual improvement of the quality of life and the enhancement of environmental awareness, people pay more and more attention to living environment, and the prevention and treatment of plant diseases also begin to find safer, more reliable, more economic and more effective technologies. Biological control gradually becomes a main method for replacing chemical control because of the advantages of good environmental compatibility, difficult resistance, lasting and stable control effect and the like, the research on biological control focuses on searching for biocontrol bacteria resources and developing and researching novel biocontrol microorganisms, while bacillus, especially bacillus subtilis, has been the hot spot of research as a beneficial biocontrol bacterium, bacillus is one of the earliest bacteria found in human beings, as early as 1835, Ehrenberg described "vibrio subtilis" which is now familiar with "bacillus subtilis" formally named by Cohn in 1872, now as a model strain of the family bacillaceae, and has typical bacillus characteristics in terms of biological properties, the cells are straight rod-shaped, have the sizes of (0.8-1.2) Mumx (1.5-4.0) Mum, are single, gram-positive, are uniformly colored, can produce capsules and can move (peritrichome); spore mesogenesis or near mesogenesis, which is less than or equal to the cell width and is elliptical to cylindrical; the colony is coarse, opaque, expanded, white or slightly yellow; the bacillus subtilis can be liquefied into gelatin, peptone milk, reduced nitrate and hydrolyzed starch, is typical aerobic bacteria, has been studied and applied for more than 100 years at home and abroad, most of early work is concentrated on aspects of morphological observation, classification identification, physiological mechanism, function discovery, prevention and control and the like, in recent years, the research on the chemical book bacillus subtilis is gradually developed into the fields of genetics and molecular biology, research contents are embodied in the aspects of searching and cloning specific functional genes into required species or genetic modification on bacillus subtilis producing bacteria by means of mutagenesis, genetic engineering and the like, along with the deep development of people on the research on the bacillus subtilis, the bacillus subtilis is found to have wide application values in the aspects of industry, agriculture, medical sanitation, food health care, aquaculture and the like, belongs to bacillus and is a thermophilic aerobic bacillus, Gram-positive bacteria, which are endogenous resistant to spores, are widely distributed in soil and putrefactive organisms, and have been studied for over 50 years as typical representatives of gram-positive bacteria in physiology, biochemistry, heredity and molecular biology. The bacillus subtilis is one of the common host bacteria for transgenic technology, and other common host bacteria include escherichia coli, saccharomycetes, agrobacterium and the like. The bacillus subtilis is also a probiotic, is applied to a plurality of fields as a probiotic, can generate an antibiotic with a certain antibacterial effect in the growth process in the aspect of food industry, and can be used as a zymogen of food products as the bacillus subtilis does not generate substances harmful to human bodies through research verification; in agriculture, bacillus subtilis is widely used as an effective biocontrol bacterium, can inhibit the growth of pathogenic bacteria and can promote the yield increase of crops; the bacillus subtilis can also be applied to industries such as feed, medicine (novel medicine or antigen delivery system and the like), breeding industry, washing and textile leather and the like at present, and can also be applied to various fields as a microecological preparation.
However, in the production process of the bacillus subtilis microbial preparation in the current market, the most important step is a fermentation step, and a fermentation device of the bacillus subtilis microbial preparation in the current market has a large defect in the fermentation process, and the problems of culture medium precipitation and caking can occur in the fermentation process of the culture medium, so that the fermentation speeds of the culture mediums at different parts in the fermentation tank are different, and therefore a user can not accurately control the fermentation time of the bacillus subtilis microbial preparation, and the quality of the bacillus subtilis microbial preparation is low.
Disclosure of Invention
The invention aims to: in order to solve the above problems, a fermentation apparatus for a bacillus subtilis microbial preparation is provided.
In order to achieve the purpose, the invention provides the following technical scheme: a fermentation device of a bacillus subtilis microbial preparation comprises a fermentation tank, wherein a sealing cover is installed at the top end of the fermentation tank, a mixing mechanism is arranged inside the sealing cover, a corrugated pipe is fixed inside the fermentation tank, the bottom end of the corrugated pipe is connected with a mixing tank, a spraying mechanism is installed on the outer wall of the mixing tank, and an oxygen supply mechanism is fixed at the bottom end of the mixing tank;
the mixing mechanism comprises a motor connected with a sealing cover, a first rotating rod is fixed at the bottom end of the motor, a first gear disc is connected to the outer wall of the first rotating rod, a second gear disc is arranged on the outer wall of the first gear disc, a gear sleeve is arranged on the outer wall of the second gear disc, a second rotating rod is fixed inside the second gear disc, a first helical gear is connected to the top end of the second rotating rod, a second helical gear is arranged on the outer wall of the first helical gear, a third rotating rod is arranged inside the second helical gear, a rotating disc is fixed at one end, away from the second helical gear, of the third rotating rod, a second connecting rod is connected inside the rotating disc, a rotating disc is arranged at one end, away from the rotating disc, of the second connecting rod, a third connecting rod is arranged at the bottom end of the rotating disc, and a lifting rod is connected to the outer, the bottom end of the lifting rod is fixedly provided with a connecting block, the outer wall of the first rotating rod is provided with a spiral blade, the outer wall of the spiral blade is provided with an upper charging barrel, and the outer wall of the upper charging barrel is connected with a stirring blade;
the spraying mechanism comprises a clamping strip connected with the mixing tank, a third gear disc is arranged on the outer wall of the clamping strip, a first gear shaft is fixed inside the third gear disc, a first chain is arranged on one side, located on the first gear shaft, of the outer wall of the first gear shaft, a second gear shaft is connected to the upper portion, located on the first gear shaft, of the inner wall of the first chain, a second chain is arranged on one side, located on the first chain, of the outer wall of the second gear shaft, a third gear shaft is connected to one side, located on the second gear shaft, of the inner wall of the second chain, a rotating block is fixed at the bottom end of the third gear shaft, an atomizing nozzle is arranged at the bottom end of the rotating block, and the input end of the atomizing nozzle is connected with;
the oxygen suppliment mechanism is including the lift seat that links to each other with the blending tank, the bottom mounting of lift seat has the piston board, the outer wall connection of piston board has the piston pipe, first connecting pipe is installed to one side of piston pipe, one side that the piston pipe was kept away from to first connecting pipe is provided with the oxygen jar, the one end of piston pipe is fixed with the second connecting pipe, the output of second connecting pipe is provided with waterproof ventilated membrane.
Preferably, first rotation pole is including the telescopic link that links to each other with helical blade, the top of telescopic link is connected with the stop collar, spacing dish is installed to the inner wall of telescopic link, the top of spacing dish is fixed with the head rod, the outer wall of head rod is provided with the limiting plate.
Preferably, the number of the second gear discs is four, the outer walls of the first gear discs are meshed with the outer walls of the four second gear discs through clamping teeth, the outer walls of the second gear discs are meshed with the inner wall of the gear sleeve through clamping teeth, and the first gear disc rotates six circles to drive the second gear disc to rotate one circle.
Preferably, the inner wall bottom of blending tank is provided with the annular spout with second dwang assorted, the bottom and the annular spout of second dwang pass through the bearing and rotate and be connected, the inner wall bottom of first dwang and blending tank passes through the bearing and rotates and be connected.
Preferably, the number of the feeding barrels is five groups, four groups of the feeding barrels are fixedly connected with four groups of second rotating rods, and the other group of the feeding barrels are fixedly connected with the first rotating rods.
Preferably, the quantity of oxygen suppliment mechanism is two sets of, two sets of the internally mounted of oxygen suppliment mechanism has four waterproof ventilated membranes of group, four groups waterproof ventilated membrane evenly distributed is in the inner wall of blending tank, the inside of second connecting pipe is provided with the check valve, the inside of first connecting pipe is provided with the check valve.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, by arranging the fermentation tank, the sealing cover, the mixing mechanism and the mixing tank, a user puts materials into the mixing tank through the feeding hole at the top end of the sealing cover, after the materials are completely filled, the feeding hole at the top end of the sealing cover is sealed, then a power switch of a motor is started, the output end of the motor drives the first rotating rod to rotate, so that the spiral blade is driven to rotate, the spiral blade rotates to drive the materials at the bottom end of the mixing tank to move upwards, and meanwhile, larger materials are crushed; when the first rotating rod drives the spiral blade to rotate, the upper charging barrel is driven to rotate, the upper charging barrel rotates to drive the stirring blade to rotate, so that materials in the mixing tank are driven to rotate, the spiral blade moves the materials at the bottom end of the mixing tank upwards, and the stirring blade drives the materials in the mixing tank to rotate, so that the materials in the mixing tank are uniformly stirred; when the first rotating rod drives the spiral blades to rotate, the first gear disc is driven to rotate, the first gear disc drives the second gear disc to rotate, so that the second rotating rod is driven to rotate, the second rotating rod drives the first helical gear to rotate, so that the second helical gear is driven to rotate, the second helical gear drives the third rotating rod to rotate, so that the rotating disk is driven to rotate, the rotating disk drives the second connecting rod to rotate, so that the rotating disk moves, so that the third connecting rod moves, so that the lifting rod is driven to do lifting reciprocating motion, the lifting rod moves to drive the connecting block to move, so that the mixing tank is driven to do lifting reciprocating motion, the internal materials are vibrated, the caked materials are shaken and dispersed, and the materials can be fermented better;
2. according to the invention, by arranging the fermentation tank, the sealing cover, the mixing mechanism, the mixing tank and the oxygen supply mechanism, when the mixing tank does lifting reciprocating motion, the lifting seat at the bottom end of the mixing tank is driven to move, so that the piston plate is driven to do vertical reciprocating motion, when the piston plate goes upwards, oxygen in the oxygen tank is pumped and driven to enter the piston tube through the first connecting pipe, when the piston plate goes downwards, the oxygen in the piston tube is driven to move, the oxygen enters the mixing tank through the second connecting pipe and the waterproof breathable films, and the second connecting pipe and the waterproof breathable films are multiple in number and are uniformly distributed at the bottom end of the inner wall of the mixing tank, so that materials in the mixing tank are fully contacted with the oxygen, the material oxidation reaction is more uniform, and;
3. the invention is provided with the fermentation tank, the sealing cover, the mixing mechanism, the mixing tank and the spraying mechanism, when the mixing tank drives the lifting seat to move, the clamping strip is driven to move, thereby driving the third gear plate to rotate, the third gear plate drives the first gear shaft to rotate, thereby driving the first chain to move, the first chain drives the second gear shaft to rotate, thereby driving the second chain to move, the second chain drives the third gear shaft to rotate, thereby driving the rotating block to rotate, the rotating block drives the atomizing nozzle to move, when a user needs to spray water on the materials, the valve of the water inlet pipe is opened, the water inlet pipe conveys the external water source to the atomizing nozzle, when the atomizing spray head is used for spraying water on the materials, the materials are in a mixing and stirring state due to the steps, the atomizing nozzle is in a reciprocating swing state, so that the materials in the mixing tank are uniformly mixed with water;
4. the invention is provided with a fermentation tank, a sealing cover, a mixing mechanism, a mixing tank, a spraying mechanism, an oxygen supply mechanism and a corrugated pipe, after the materials in the mixing tank are mixed, the power switch of the motor is closed, the valve of the water inlet pipe is closed, the mixing tank is in a static state, the materials in the mixing tank are gradually calmed down, so that partial materials in the mixing tank begin to precipitate and ferment, when the materials at the bottom end of the mixing tank begin to agglomerate after the fermentation is carried out for a fixed time, the materials in the mixing tank begin to ferment unevenly, at the moment, the mixing mechanism is started again to transport the deposited and agglomerated materials at the bottom end of the mixing tank to the upper part of the mixing tank through the helical blades, and the materials in the mixing tank are stirred and mixed again, the uniformity of material fermentation is ensured, the whole fermentation progress of the material in the fermentation tank is conveniently and accurately controlled by a user, and therefore the production quality of the bacillus subtilis microbial preparation is higher.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural diagram of a mixing mechanism of the present invention;
FIG. 3 is a schematic structural view of a rotary disk according to the present invention;
FIG. 4 is a schematic structural view of a helical blade according to the present invention;
FIG. 5 is a schematic structural diagram of the first rotating rod of the present invention;
FIG. 6 is a schematic structural view of the spray mechanism of the present invention;
fig. 7 is a schematic diagram of the mechanism of the oxygen supply mechanism of the present invention.
In the figure: 1. a fermentation tank; 2. a sealing cover; 3. a mixing mechanism; 301. a motor; 302. a first rotating lever; 3021. a first connecting rod; 3022. a limiting plate; 3023. a limiting disc; 3024. a telescopic rod; 3025. a limiting sleeve; 303. a first gear plate; 304. a second gear wheel disc; 305. a gear sleeve; 306. a second rotating lever; 307. a first helical gear; 308. a second helical gear; 309. a third rotating rod; 310. rotating the disc; 311. a second connecting rod; 312. rotating the disc; 313. a third connecting rod; 314. a lifting rod; 315. connecting blocks; 316. a helical blade; 317. feeding a material barrel; 318. stirring blades; 4. a mixing tank; 5. a spraying mechanism; 501. clamping the strip; 502. a third gear plate; 503. a first gear shaft; 504. a first chain; 505. a second gear shaft; 506. a second chain; 507. a third gear shaft; 508. rotating the block; 509. an atomizing spray head; 510. a water inlet pipe; 6. an oxygen supply mechanism; 601. a lifting seat; 602. a piston plate; 603. a piston tube; 604. a first connecting pipe; 605. an oxygen tank; 606. a second connecting pipe; 607. a waterproof breathable film; 7. a bellows.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. The following describes an embodiment of the present invention based on its overall structure.
Referring to fig. 1-7, a fermentation apparatus for bacillus subtilis microbial preparation comprises a fermentation tank 1, a sealing cover 2 is installed at the top end of the fermentation tank 1, a mixing mechanism 3 is arranged inside the sealing cover 2, a corrugated pipe 7 is fixed inside the fermentation tank 1, the bottom end of the corrugated pipe 7 is connected with a mixing tank 4, a spraying mechanism 5 is installed on the outer wall of the mixing tank 4, and an oxygen supply mechanism 6 is fixed at the bottom end of the mixing tank 4;
the mixing mechanism 3 comprises a motor 301 connected with the sealing cover 2, a first rotating rod 302 is fixed at the bottom end of the motor 301, a first gear disc 303 is connected with the outer wall of the first rotating rod 302, a second gear disc 304 is arranged on the outer wall of the first gear disc 303, a gear sleeve 305 is arranged on the outer wall of the second gear disc 304, a second rotating rod 306 is fixed inside the second gear disc 304, a first helical gear 307 is connected with the top end of the second rotating rod 306, a second helical gear 308 is arranged on the outer wall of the first helical gear 307, a third rotating rod 309 is arranged inside the second helical gear 308, a rotating disc 310 is fixed at one end of the third rotating rod 309 far away from the second helical gear 308, a second connecting rod 311 is connected inside the rotating disc 310, a rotating disc 312 is arranged at one end of the second connecting rod 311 far away from the rotating disc 310, a third connecting rod 313 is arranged at the bottom end of the rotating disc 312, a connecting block 315 is fixed at the bottom end of the lifting rod 314, a spiral blade 316 is installed on the outer wall of the first rotating rod 302, an upper charging barrel 317 is arranged on the outer wall of the spiral blade 316, and a stirring blade 318 is connected to the outer wall of the upper charging barrel 317;
the spraying mechanism 5 comprises a clamping bar 501 connected with the mixing tank 4, a third gear disc 502 is arranged on the outer wall of the clamping bar 501, a first gear shaft 503 is fixed inside the third gear disc 502, a first chain 504 is arranged on one side, located on the first gear shaft 503, of the outer wall of the first gear shaft 503, a second gear shaft 505 is connected to the upper portion, located on the first gear shaft 503, of the inner wall of the first chain 504, a second chain 506 is arranged on one side, located on the first chain 504, of the outer wall of the second gear shaft 505, a third gear shaft 507 is connected to one side, located on the second gear shaft 505, of the inner wall of the second chain 506, a rotating block 508 is fixed to the bottom end of the third gear shaft 507, an atomizing nozzle is arranged at the bottom end of the rotating block 509, and the input end;
the oxygen supply mechanism 6 comprises a lifting seat 601 connected with the mixing tank 4, a piston plate 602 is fixed at the bottom end of the lifting seat 601, a piston pipe 603 is connected to the outer wall of the piston plate 602, a first connecting pipe 604 is installed on one side of the piston pipe 603, an oxygen tank 605 is arranged on one side, away from the piston pipe 603, of the first connecting pipe 604, a second connecting pipe 606 is fixed at one end of the piston pipe 603, and a waterproof breathable film 607 is arranged at the output end of the second connecting pipe 606.
Referring to fig. 1-5, the first rotating rod 302 includes an expansion rod 3024 connected to the spiral blade 316, a limiting sleeve 3025 is connected to the top end of the expansion rod 3024, a limiting disc 3023 is installed on the inner wall of the expansion rod 3024, a first connecting rod 3021 is fixed to the top end of the limiting disc 3023, and a limiting plate 3022 is installed on the outer wall of the first connecting rod 3021, so that the first rotating rod 302 can move up and down in a reciprocating manner when the mixing tank 4 moves up and down in a reciprocating manner.
Please refer to fig. 1 and fig. 2, the number of the second gear discs 304 is four, the outer wall of the first gear disc 303 is engaged with the outer walls of the four second gear discs 304 through the teeth, the outer wall of the second gear disc 304 is engaged with the inner wall of the gear sleeve 305 through the teeth, the first gear disc 303 rotates six turns to drive the second gear disc 304 to rotate one turn, so that the first gear disc 303 rotates at a high speed and drives the second gear disc 304 to rotate slowly.
Please refer to fig. 1 and fig. 2, the bottom end of the inner wall of the mixing tank 4 is provided with an annular sliding groove matched with the second rotating rod 306, the bottom end of the second rotating rod 306 is rotatably connected with the annular sliding groove through a bearing, and the first rotating rod 302 is rotatably connected with the bottom end of the inner wall of the mixing tank 4 through a bearing, so that the stability between the second rotating rod 306 and the mixing tank 4 is increased.
Please refer to fig. 2 and fig. 4, the number of the upper material cylinders 317 is five, the number of the second rotating rods 306 is four, the four upper material cylinders 317 are fixedly connected with the four second rotating rods 306, the other upper material cylinder 317 is fixedly connected with the first rotating rod 302, so that the helical blade 316 drives the bottom layer culture medium to move upwards, and the rotation of the outer wall stirring blade 318 makes the culture medium in the mixing tank 4 stir more fully.
Please refer to fig. 1 and fig. 7, the number of the oxygen supply mechanisms 6 is two, four sets of waterproof air permeable membranes 607 are installed inside the two sets of oxygen supply mechanisms 6, the four sets of waterproof air permeable membranes 607 are uniformly distributed on the inner wall of the mixing tank 4, the one-way valve is installed inside the second connecting pipe 606, and the one-way valve is installed inside the first connecting pipe 604, so that the oxygen inside the oxygen tank 605 can be unidirectionally transported to the inside of the mixing tank 4 after passing through the first connecting pipe 604, the piston pipe 603 and the second connecting pipe 606.
The working principle is as follows: firstly, a user puts a material into the mixing tank 4 through a feeding hole at the top end of the sealing cover 2, after the material is filled, the feeding hole at the top end of the sealing cover 2 is sealed, then a power switch of the motor 301 is started, the output end of the motor 301 drives the first rotating rod 302 to rotate, so that the spiral blade 316 is driven to rotate, the spiral blade 316 rotates to drive the material at the bottom end of the mixing tank 4 to move upwards, and meanwhile, larger materials are crushed;
when the first rotating rod 302 drives the helical blade 316 to rotate and simultaneously drives the upper charging barrel 317 to rotate, the upper charging barrel 317 rotates to drive the stirring blade 318 to rotate, so that the materials in the mixing tank 4 are driven to rotate, and the helical blade 316 moves the materials at the bottom end of the mixing tank 4 upwards, and the stirring blade 318 drives the materials in the mixing tank 4 to rotate, so that the materials in the mixing tank 4 are uniformly stirred;
when the first rotating rod 302 drives the spiral blade 316 to rotate and simultaneously drives the first gear disc 303 to rotate, the first gear disc 303 rotates and drives the second gear disc 304 to rotate, so as to drive the second rotating rod 306 to rotate, the second rotating rod 306 rotates and drives the first bevel gear 307 to rotate, so as to drive the second bevel gear 308 to rotate, the second bevel gear 308 rotates and drives the third rotating rod 309 to rotate, so as to drive the rotating disc 310 to rotate, the rotating disc 310 rotates and drives the second connecting rod 311 to rotate, so as to drive the rotating disc 312 to move, the rotating disc 312 moves and drives the third connecting rod 313 to move, so as to drive the lifting rod 314 to do lifting reciprocating motion, the lifting rod 314 moves and drives the connecting block 315 to move, so as to drive the mixing tank 4 to do lifting reciprocating motion, so that the internal material is vibrated, so that the caked material is shaken and;
when the mixing tank 4 does lifting reciprocating motion, the lifting seat 601 at the bottom end of the mixing tank is driven to move, so that the piston plate 602 is driven to do vertical reciprocating motion, when the piston plate 602 goes upwards, oxygen in the oxygen tank 605 is pumped and driven to enter the piston pipe 603 through the first connecting pipe 604, when the piston plate 602 goes downwards, the oxygen in the piston pipe 603 is driven to move, the oxygen enters the mixing tank 4 through the second connecting pipe 606 and the waterproof breathable films 607, and because the second connecting pipes 606 and the waterproof breathable films are in multiple groups and are uniformly distributed at the bottom end of the inner wall of the mixing tank 4, materials in the mixing tank 4 are fully contacted with the oxygen, so that the material oxidation reaction is more uniform, and the fermentation effect is better;
when the mixing tank 4 drives the lifting seat 601 to move and simultaneously drives the clamping strip 501 to move, so as to drive the third gear plate 502 to rotate, the third gear plate 502 rotates to drive the first gear shaft 503 to rotate, so as to drive the first chain 504 to move, the first chain 504 moves to drive the second gear shaft 505 to rotate, so as to drive the second chain 506 to move, the second chain 506 moves to drive the third gear shaft 507 to rotate, so as to drive the rotating block 508 to rotate, the rotating block 508 rotates to drive the atomizing nozzle 509 to move, when a user needs to sprinkle water on a material, a valve of the water inlet pipe 510 is opened, the water inlet pipe 510 transports an external water source to the atomizing nozzle 509, and when the material is sprinkled through the atomizing nozzle 509, the material is in a mixing and stirring state due to the steps, and the atomizing nozzle 509 is in a reciprocating swing state, so as to uniformly mix the material and the water in the;
after the mixing of the inside material of blending tank 4 finishes, close motor 301's switch, close the valve of inlet tube 310, blending tank 4 is in quiescent condition this moment, the inside material of blending tank 4 calms down gradually, thereby lead to partial material to begin to precipitate in blending tank 4, the fermentation, after fermentation fixed time, 4 bottom materials of blending tank begin to agglomerate, it is inhomogeneous to lead to 4 inside material fermentations of blending tank, restart mixing mechanism 3 at this moment transports 4 bottom deposit caking materials of blending tank to 4 tops of blending tank through helical blade 316, and stir the mixture once more to 4 inside materials of blending tank, with the homogeneity of guarantee material fermentation, the whole fermentation progress of 1 inside material of convenient to use person accurate control fermentation cylinder, thereby make bacillus subtilis microbial preparation's production quality higher.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. A fermenting installation of bacillus subtilis microbial preparation, includes fermentation cylinder (1), its characterized in that: the top end of the fermentation tank (1) is provided with a sealing cover (2), a mixing mechanism (3) is arranged inside the sealing cover (2), a corrugated pipe (7) is fixed inside the fermentation tank (1), the bottom end of the corrugated pipe (7) is connected with a mixing tank (4), the outer wall of the mixing tank (4) is provided with a spraying mechanism (5), and the bottom end of the mixing tank (4) is fixed with an oxygen supply mechanism (6);
the mixing mechanism (3) comprises a motor (301) connected with a sealing cover (2), a first rotating rod (302) is fixed at the bottom end of the motor (301), a first gear disc (303) is connected to the outer wall of the first rotating rod (302), a second gear disc (304) is arranged on the outer wall of the first gear disc (303), a gear sleeve (305) is installed on the outer wall of the second gear disc (304), a second rotating rod (306) is fixed inside the second gear disc (304), a first helical gear (307) is connected to the top end of the second rotating rod (306), a second helical gear (308) is installed on the outer wall of the first helical gear (307), a third rotating rod (309) is arranged inside the second helical gear (308), a rotating disc (310) is fixed at one end, far away from the second helical gear (308), of the third rotating rod (309), and a second connecting rod (311) is connected inside the rotating disc (310), a rotating disc (312) is mounted at one end, away from the rotating disc (310), of the second connecting rod (311), a third connecting rod (313) is arranged at the bottom end of the rotating disc (312), a lifting rod (314) is connected to the outer wall of the third connecting rod (313), a connecting block (315) is fixed to the bottom end of the lifting rod (314), a spiral blade (316) is mounted on the outer wall of the first rotating rod (302), an upper charging barrel (317) is arranged on the outer wall of the spiral blade (316), and a stirring blade (318) is connected to the outer wall of the upper charging barrel (317);
the spraying mechanism (5) comprises a clamping strip (501) connected with the mixing tank (4), a third gear disc (502) is arranged on the outer wall of the clamping strip (501), a first gear shaft (503) is fixed inside the third gear disc (502), a first chain (504) is installed on one side, located on the first gear shaft (503), of the outer wall of the first gear shaft (503), a second gear shaft (505) is connected to the upper portion, located on the first gear shaft (503), of the inner wall of the first chain (504), a second chain (506) is arranged on one side, located on the first chain (504), of the outer wall of the second gear shaft (505), a third gear shaft (507) is connected to one side, located on the second gear shaft (505), of the inner wall of the second chain (506), a rotating block (508) is fixed at the bottom end of the third gear shaft (507), and an atomizing spray head (509) is installed at the bottom end of the rotating block (508), the input end of the atomizing nozzle (509) is connected with a water inlet pipe (510);
oxygen suppliment mechanism (6) are including lift seat (601) that link to each other with blending tank (4), the bottom mounting of lift seat (601) has piston board (602), the outer wall connection of piston board (602) has piston pipe (603), first connecting pipe (604) are installed to one side of piston pipe (603), one side that piston pipe (603) were kept away from in first connecting pipe (604) is provided with oxygen jar (605), the one end of piston pipe (603) is fixed with second connecting pipe (606), the output of second connecting pipe (606) is provided with waterproof ventilated membrane (607).
2. The apparatus for fermenting a microbial preparation of bacillus subtilis according to claim 1, wherein: first pivot pole (302) including telescopic link (3024) that link to each other with helical blade (316), the top of telescopic link (3024) is connected with stop collar (3025), spacing dish (3023) are installed to the inner wall of telescopic link (3024), the top of spacing dish (3023) is fixed with head rod (3021), the outer wall of head rod (3021) is provided with limiting plate (3022).
3. The apparatus for fermenting a microbial preparation of bacillus subtilis according to claim 1, wherein: the number of second toothed disc (304) is four groups, first toothed disc (303) outer wall passes through the latch meshing with four groups second toothed disc (304) outer walls, the outer wall of second toothed disc (304) passes through the latch meshing with the inner wall of gear sleeve (305), first toothed disc (303) rotate six rings and drive second toothed disc (304) and rotate the round.
4. The apparatus for fermenting a microbial preparation of bacillus subtilis according to claim 1, wherein: the inner wall bottom of blending tank (4) is provided with the annular spout with second dwang (306) assorted, the bottom and the annular spout of second dwang (306) pass through the bearing and rotate and be connected, first dwang (302) pass through the bearing rotation with the inner wall bottom of blending tank (4) and are connected.
5. The apparatus for fermenting a microbial preparation of bacillus subtilis according to claim 1, wherein: go up feed cylinder (317) quantity and be five groups, four groups go up feed cylinder (317) and four groups second dwang (306) fixed connection, another group go up feed cylinder (317) and first dwang (302) fixed connection.
6. The apparatus for fermenting a microbial preparation of bacillus subtilis according to claim 1, wherein: the quantity of oxygen suppliment mechanism (6) is two sets of, two sets of the internally mounted of oxygen suppliment mechanism (6) has four waterproof ventilated membrane of group (607), four groups waterproof ventilated membrane (607) evenly distributed is in the inner wall of blending tank (4), the inside of second connecting pipe (606) is provided with the check valve, the inside of first connecting pipe (604) is provided with the check valve.
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KR100751792B1 (en) * 2007-02-16 2007-08-23 오봉길 Fermenter
KR20080097893A (en) * 2007-05-02 2008-11-06 최병권 Treatment system of organic waste
CN109678575A (en) * 2019-02-18 2019-04-26 蚌埠学院 A kind of agricultural wastes aerobic composting fermentation case
CN110229741A (en) * 2019-06-21 2019-09-13 徐光飞 A kind of fermentation mixing arrangement with self-cleaning function for Chemical Manufacture
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CN211311451U (en) * 2019-07-11 2020-08-21 陆良佰佳益康生物科技有限公司 Fermentation cylinder of bacillus subtilis production
CN211688986U (en) * 2019-11-07 2020-10-16 武汉光华时代生物科技有限公司 Preparation facilities of bacillus subtilis zymotic fluid suspension
CN111825489A (en) * 2020-06-28 2020-10-27 周谢保 Bio-organic fertilizer mixing fermentation cylinder
CN211814194U (en) * 2020-03-02 2020-10-30 山东土秀才生物科技有限公司 High-efficient microbial fermentation equipment
CN111979104A (en) * 2020-09-01 2020-11-24 威海惠高生物科技有限公司 Functional health-care red yeast rice fermentation preparation process and preparation device thereof

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100751792B1 (en) * 2007-02-16 2007-08-23 오봉길 Fermenter
KR20080097893A (en) * 2007-05-02 2008-11-06 최병권 Treatment system of organic waste
CN109678575A (en) * 2019-02-18 2019-04-26 蚌埠学院 A kind of agricultural wastes aerobic composting fermentation case
CN110229741A (en) * 2019-06-21 2019-09-13 徐光飞 A kind of fermentation mixing arrangement with self-cleaning function for Chemical Manufacture
CN211311451U (en) * 2019-07-11 2020-08-21 陆良佰佳益康生物科技有限公司 Fermentation cylinder of bacillus subtilis production
CN210826179U (en) * 2019-10-30 2020-06-23 湖南普菲克生物科技有限公司 Solid-state fermentation device for biological feed
CN211688986U (en) * 2019-11-07 2020-10-16 武汉光华时代生物科技有限公司 Preparation facilities of bacillus subtilis zymotic fluid suspension
CN211814194U (en) * 2020-03-02 2020-10-30 山东土秀才生物科技有限公司 High-efficient microbial fermentation equipment
CN111825489A (en) * 2020-06-28 2020-10-27 周谢保 Bio-organic fertilizer mixing fermentation cylinder
CN111979104A (en) * 2020-09-01 2020-11-24 威海惠高生物科技有限公司 Functional health-care red yeast rice fermentation preparation process and preparation device thereof

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