CN112898957B

CN112898957B - Carrying fluid and preparation method thereof

Info

Publication number: CN112898957B
Application number: CN202110068856.2A
Authority: CN
Inventors: 冯青; 李啸南; 佘跃惠; 张凡; 黄子俊; 李凡; 曾鸣; 杨慰兴; 魏志鹏; 樊爱彬; 姜杰; 高杰; 田苗; 蔡依娜; 王绪性; 苏三宝; 董浩; 冯阳阳
Original assignee: China Oilfield Services Ltd
Current assignee: China Oilfield Services Ltd
Priority date: 2021-01-19
Filing date: 2021-01-19
Publication date: 2022-10-28
Anticipated expiration: 2041-01-19
Also published as: CN112898957A

Abstract

The application discloses a nano silicon material carrying fluid, which comprises the following components in percentage by weight: 0.1 to 1 percent of biosurfactant, 0.1 to 2 percent of biological enzyme, 0.5 to 2 percent of cosolvent, 0.01 to 0.1 percent of microbial polysaccharide and the balance of water. The hydrophobic nano polysilicon material can be well dispersed in the carrying fluid and keeps very good stability. The carrying fluid is green and environment-friendly, can be completely biodegraded, can be stable for a long time when the hydrophobic nano material is dispersed in the carrying fluid, enters the oil field layer under the high temperature and multivalent ion environment, is firmly adsorbed on the surface of rock after demulsification, changes the wettability of the rock, reduces the water injection pressure and improves the water injection quantity.

Description

Carrying fluid and preparation method thereof

Technical Field

The invention relates to but is not limited to an oil field working fluid, in particular to but not limited to a carrying fluid and a preparation method thereof.

Background

For the development of oil fields by water injection, along with the extension of water injection time, the surface of rock pores in the near wellbore zone is hydrated and expanded to block a throat, so that the water injection pressure is increased, and even the development is carried out to the ground without water injection. Most oil fields increase the water injection rate by improving the water injection pressure, but the water injection pressure cannot exceed the bearing capacity of equipment and oil reservoirs, so the problem cannot be solved fundamentally only by improving the water injection pressure to increase the water injection rate, and hidden dangers are brought to safe production. The conventionally adopted drag reduction and injection increase method is to implement acidification operation on a water injection well, and aims to remove pollution of a near-wellbore area and an expanded layer on the surface of rock pores and play a role in reducing pressure and increasing injection, but the method has short effective period, mostly less than three months, frequent operation, poor use effect in medium and low permeability oil reservoirs, high cost and environmental pollution. The surfactant is adopted to reduce the pressure and increase the injection, and the problem of short validity period and the like also exists.

Compared with other oil field injection increasing technologies, the pressure-reducing injection-increasing effect of the polysilicon nano injection increasing technology is mainly based on physical effects, and the processing technology of the polysilicon nano material is relatively simple and has no pollution to the ground and the stratum environment. The technology is suitable for the pressure reduction and injection increase of the water injection well of the medium and low permeability oil reservoir. The injected silicon material is adsorbed on the rock surface of an oil layer, so that the rock surface is changed from water wetting to oil wetting, the dynamic action of fluid and the rock surface is improved, the seepage resistance is reduced, and the method has the advantages of obvious effect, simplicity in construction, no pollution and the like. However, the process needs diesel oil to carry, the dosage is large, the cost is high, and the diesel oil has various safety problems in the transportation, storage and use processes, which brings great difficulty to the popularization of the technology.

In recent years, a water-based microemulsion system of polysilicon nano powder is prepared by uniformly dispersing a super-hydrophobic nano polysilicon material in water by selecting a compound chemical surfactant. The system has good dispersibility and stability, and can replace diesel oil as a carrying medium to bring the nano-silicon material into the underground. However, the hydrophobic nano water-based polysilicon carrier fluid system prepared by chemical surfactants such as SDS, TX-10, CTAB and the like needs ultrasonic dispersion or high-speed stirring, such as 5000-8000 rpm, and hot water with the temperature of more than 80 ℃ and is inconvenient to prepare in an oil field. If the water-based nanodispersions are prepared in advance in a factory or a solution preparation station, they cannot be stabilized for a long period of time. In addition, the chemical surfactant has the problems of difficult microbial degradation, safety, environmental protection and the like.

Disclosure of Invention

The following is a summary of the subject matter described in detail herein. This summary is not intended to limit the scope of the present application.

The application provides a bio-based hydrophobic polysilica nano-material carrier fluid used for increasing injection of an oil field water injection well, which is safe and environment-friendly, replaces a diesel oil or chemical surfactant system, carries and injects hydrophobic polysilica nano-powder material into the water injection well, reduces construction cost, eliminates potential safety hazards, is green and environment-friendly, and has the characteristic of sustainable development.

The application provides a nano silicon material carrying fluid, which comprises the following components in percentage by weight: 0.1 to 1 percent of biosurfactant, 0.1 to 2 percent of biological enzyme, 0.5 to 2 percent of cosolvent, 0.01 to 0.1 percent of microbial polysaccharide and the balance of water.

In exemplary embodiments provided herein, the biosurfactant is selected from any one or more of rhamnolipids, lipopeptides, sterols, lipoproteins and sophorolipids.

In exemplary embodiments provided herein, the biological enzyme is selected from any one or more of lipases, proteases, esterases, isomerases, and amylases;

in exemplary embodiments provided herein, the weight ratio of lipase, protease, esterase, isomerase, and amylase is (0.01 to 0.1): (0.01 to 1): (0.05 to 0.5): (0 to 0.5): and;

in exemplary embodiments provided herein, the lipase is selected from either or both of a Pseudomonas sp-producing microorganism and a Bacillus sp-producing microorganism;

in exemplary embodiments provided herein, the protease is selected from either or both of a protease produced by a microorganism of the genus Bacillus (Bacillus sp.) and a protease produced by a microorganism of the genus brevibacterium (Curtobacterium sp.);

in exemplary embodiments provided herein, the esterase is selected from either or both of an esterase produced by a microorganism of the genus Acinetobacter (Acinetobacter sp.) and an esterase produced by a microorganism of the genus Pseudomonas (Pseudomonas sp.);

in exemplary embodiments provided herein, the isomerase is selected from any one or two of an isomerase produced by a microorganism of the genus Rhodobacter (Rhodobacter sp.), an isomerase produced by a microorganism of the genus acidophilic lactobacillus (Lact obacillus acidophilus sp.);

in exemplary embodiments provided herein, the amylase is selected from any one or both of a Bacillus (Bacillus sp.) microorganism amylase, halomonas (Halomonas) amylase.

In exemplary embodiments provided herein, the lipopeptide may be selected from the group consisting of a lipopeptide produced by Bacillus subtilis; the sterol may be selected from cholesterol;

in exemplary embodiments provided herein, the lipoprotein may be selected from the group consisting of a Pseudomonas aeruginosa (Pseudomonas aeruginosa) lipoprotein.

In exemplary embodiments provided herein, the co-solvent is a low molecular weight alcohol;

in exemplary embodiments provided herein, the co-solvent is selected from any one or more of propanol, isopropanol, butanol, pentanol, and isoamyl alcohol.

In exemplary embodiments provided herein, the microbial polysaccharide is selected from either or both of lactobacillus sp and xanthomonas sp microbial biomacromolecule microbial polysaccharides.

When in use, the weight ratio of the nano carrying fluid to the nano silicon material is 100 (0.01 to 10), and optionally, the weight ratio of the nano carrying fluid to the nano silicon material is 100 (0.01 to 0.15).

In exemplary embodiments provided herein, the microbial polysaccharide is selected from exopolymers produced by a microorganism;

in an exemplary embodiment provided herein, the microbial polysaccharide is xanthan gum.

In exemplary embodiments provided herein, the nano-silicon material is a hydrophobic nano-polysilicon material;

in exemplary embodiments provided herein, the nano-silicon material is selected from any one or more of tert-butyl silane, vinyl silane, amino silane, and siloxane coupling agent modified silicon materials.

On the other hand, the present application provides a preparation method of the above nanosilicon material carrier fluid, the preparation method including: uniformly stirring the raw materials, heating to 25-40 ℃, and preserving heat for 0-2h; cooling to obtain the nano silicon material carrying fluid.

In an exemplary embodiment provided herein, the rotation speed of the stirring is 100 to 180 revolutions per minute, and the stirring time is 10 to 60 minutes.

In the exemplary embodiments provided herein, the raw materials are added to the vessel in the order of water, biosurfactant, bio-enzyme, co-solvent and microbial polysaccharide.

In another aspect, the application provides the use of biosurfactant and biological enzyme in the carrier fluid of the nano-silicon material;

in exemplary embodiments provided herein, the biosurfactant is present in an amount of 0.1wt.% to 1wt.%, and the biological enzyme is present in an amount of 0.1wt.% to 2wt.%;

in exemplary embodiments provided herein, the biosurfactant is selected from any one or more of rhamnolipids, lipopeptides, sterols, lipoproteins and sophorolipids; the biological enzyme is selected from any one or more of lipase, protease, esterase, isomerase, and amylase.

According to the bio-based carrying fluid system, the additives can be completely degraded by microorganisms, and the bio-based carrying fluid system is pollution-free to the environment and stratum and is environment-friendly. After the hydrophobic polysilica nano material is added, the viscosity is certain, the nano particles are kept to form a stable colloidal solution, the aggregation of the nano particles is reduced, the sedimentation speed is reduced, the stability can be realized for a long time, and after the nano particles enter an oil field stratum, the nano particles can be demulsified under the action of high temperature and high-valence ions to release the hydrophobic nano particles, and the nano particles are firmly adsorbed on the surface of a rock to form a compact hydrophobic layer, so that the wettability is changed, the water flow resistance is reduced, the water injection pressure is reduced, and the water injection quantity is increased. The anti-swelling effect is obvious, the anti-swelling rate of injected water is more than 80% and the anti-swelling rate of formation water is more than 90% by using the augmented liquid prepared by mixing the carrying fluid provided by the application and the nano silicon material.

Additional features and advantages of the application will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the application. Other advantages of the present application may be realized and attained by the invention in its aspects as described in the specification.

Drawings

The accompanying drawings are included to provide an understanding of the present disclosure and are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the examples serve to explain the principles of the disclosure and not to limit the disclosure.

FIG. 1 is a graph showing the distribution of particle sizes of the nano-carrier fluid after being left for different periods of time.

Fig. 2 is a graph of the measurement of the wetting angle of the surface of the core.

FIG. 3 is a graph comparing the carrying fluid obtained in example 1 with the kerosene-based carrying fluid obtained in comparative example 1 (the carrying fluid obtained in example 1 is shown on the left side, and the kerosene-based carrying fluid is shown on the right side);

FIG. 4 is a comparison graph of the carrier fluid obtained in example 1 with a comparative carrier fluid 1 obtained in comparative example 2 (the right side is the carrier fluid obtained in example 1, and the left side is the comparative carrier fluid 1);

fig. 5 is a comparison graph of the carrier fluid obtained in example 1 with a comparative carrier fluid 2 obtained in comparative example 3 (the right side is the carrier fluid obtained in example 1, and the left side is the comparative carrier fluid 2).

FIG. 6 is a graph showing the anti-swelling effect of the fluid obtained in example 1 on the clay of the reservoir, wherein the left side shows the swelling of the clay of the reservoir in water, and the right side shows the swelling of the clay of the reservoir in the fluid obtained in example 1;

FIG. 7 is a graph comparing the core barrel treated with the augmented injection solution (left core barrel) prepared in example 1 with the core barrel treated with normal tap water (right core barrel), and the experimental results show that the weight of the right core barrel is increased by 21mg and the weight of the left core barrel is increased by 6.4mg.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, embodiments of the present application are described in detail below. It should be noted that the embodiments and features of the embodiments in the present application may be arbitrarily combined with each other without conflict.

The embodiment of the application provides a nano silicon material carrying fluid, which comprises the following components in percentage by weight: 0.1 to 1 percent of biosurfactant, 0.1 to 2 percent of biological enzyme, 0.5 to 2 percent of cosolvent, 0.01 to 0.1 percent of microbial polysaccharide and the balance of water.

In embodiments of the present application, the biosurfactant is selected from any one or more of rhamnolipids, lipopeptides, sterols, lipoproteins and sophorolipids.

In embodiments herein, the biological enzyme is selected from any one or more of lipases, proteases, esterases, isomerases and amylases;

in the examples herein, the weight ratio of lipase, protease, esterase, isomerase, and amylase is (0.01 to 0.1): (0.01 to 1): (0.05 to 0.5): (0 to 0.5): 0 to 0.5);

in the present embodiment, the lipase is selected from either or both of a microorganism of the genus Pseudomonas (Pseudomonas sp.) producing lipase and a microorganism of the genus Bacillus (Bacillus sp.) producing lipase;

in the present embodiment, the protease is selected from either or both of protease produced by a microorganism of the genus Bacillus (Bacillus sp.) and protease produced by a microorganism of the genus brevibacterium (Bacillus sp.);

in the present embodiment, the esterase is selected from either or both of an esterase produced by a microorganism belonging to the genus Acinetobacter (Acinetobacter sp.) and an esterase produced by a microorganism belonging to the genus Pseudomonas sp;

in the present embodiment, the isomerase is selected from any one or two of a microorganism isomerase of the genus Rhodobacter (Rhodobacter sp.), a microorganism isomerase of the genus lactobacillus (lactobacillus acidophilus sp.);

in the examples of the present application, the amylase is selected from any one or both of amylase produced by microorganisms of the genus Bacillus (Bacillus sp.), amylase produced by Halomonas (Halomonas), and amylase produced by Bacillus sp.

In the present examples, the lipopeptide may be selected from the group consisting of a lipopeptide produced by Bacillus subtilis; the sterol may be selected from cholesterol;

in the present embodiment, the lipoprotein may be selected from the group consisting of lipoproteins produced by Pseudomonas aeruginosa (Pseudomonas aeruginosa).

In the examples herein, the co-solvent is a low molecular weight alcohol;

in embodiments herein, the co-solvent is selected from any one or more of propanol, isopropanol, butanol, pentanol and isoamyl alcohol.

In the embodiments of the present application, the microbial polysaccharide is selected from either or both of a lactic acid bacteria (lactobacillus sp.) microorganism and a xanthomonas (xanthomonas sp.) microorganism-producing biomacromolecule microbial polysaccharide.

In the present examples, the microbial polysaccharide is selected from exopolymers produced by microorganisms;

in the examples herein, the microbial polysaccharide is xanthan gum.

In the embodiment of the application, the nano silicon material is a hydrophobic nano polysilicon material;

in embodiments herein, the nano-silicon material is selected from any one or more of tert-butyl silane, vinyl silane, amino silane, and siloxane coupling agent modified silicon materials.

Example 1

In this example, the rhamnolipid is CAS:147858-26-2.

In this example, the lipase is a mixture of a microorganism belonging to the genus Pseudomonas (Pseudomonas sp.) and a microorganism belonging to the genus Bacillus (Bacillus sp.) producing lipase in a (W/W) 1:1;

the protease is protease produced by microorganisms of Bacillus (Bacillus sp.) and protease produced by microorganisms of Bacillus (Curtobacterium sp.) in a mixture manner according to (W/W) 1:1;

the esterase is a mixture of esterase produced by microorganisms of Acinetobacter (Acinetobacter sp.) and lipase produced by microorganisms of Pseudomonas (Pseudomonas sp.) in a (W/W) 1:1;

in this example, the xanthan gum was purchased from southbound engineering biotechnology limited, engineering brand;

the hydrophobic nano poly-aminosilane modified silicon material is purchased from Tantai micro-nano chemical plant, changtai CT-617 brand, shandong province Shouguang.

Preparing a bio-based hydrophobic nano carrier fluid, sequentially weighing 0.3 g of rhamnolipid, 0.4 g of lipase, 0.3 g of protease, 0.5 g of esterase, 0.5 g of propanol, 0.3 g of butanol, 0.68 g of amyl alcohol and 0.02 g of xanthan gum, sequentially dissolving the rhamnolipid, the lipase, the propanol, the butanol, the amyl alcohol and the xanthan gum in 97 g of water, stirring the mixture for 30 minutes at 100 revolutions per minute, heating the mixture to 40 ℃, preserving the heat for 2 hours, cooling the mixture to obtain 100 g of the bio-based hydrophobic nano carrier fluid, then adding 0.1 g of hydrophobic nano polysilicon material, stirring the mixture for 30 minutes at 120 revolutions per minute, and cooling the mixture for later use to form an injection fluid.

The mean particle size of the nanoparticles in the fresh carrying fluid prepared in this example was determined to be 36.68nm by a laser particle sizer (Mastersizer 3000) (figure 1).

The carrier fluid was left to stand for 24 hours, and the average particle diameter of the nanoparticles in the carrier fluid was measured to be 52.52nm (fig. 1).

The carrying fluid was left to stand for 7 days, and the average particle diameter of the nanoparticles in the carrying fluid was measured to be 53.11nm (fig. 1).

As can be seen from the above data, the nanoparticles in the carrier fluid maintained good dispersion stability, and the nanoparticle size in the carrier fluid remained at 50nm after standing for 7 days, and no precipitation coalescence occurred.

Comparative example 1

Preparing the diesel oil hydrophobic nano silicon dioxide carrying fluid, weighing 0.1 g of hydrophobic nano silicon material, dissolving in 100 g of kerosene, stirring for 30 minutes at 100 revolutions per minute, heating to 40 ℃, preserving heat for 2 hours to obtain the kerosene nano carrying fluid, and cooling for later use. As can be seen in FIG. 3, the solution of example 1 was clear with no precipitation and no flocs present; comparative example 1 kerosene-based nano-carrier fluid was turbid, precipitation occurred, and flocculation occurred.

The average particle size of the nanoparticles in the fresh carrying fluid prepared in this comparative example was determined by means of a laser particle sizer (Mastersizer 3000). It was found that the average particle diameter of the nanoparticles in the nano-sized carrier fluid of example 1 was 37.78nm, and the average particle diameter of the nanoparticles in the nano-sized carrier fluid of comparative example 1 was 127.26nm. Example 1 the nano-dispersion in the nano-carrying fluid is better than that of the kerosene-based nano-carrying fluid. Has better dispersion stability.

Comparative example 2

Preparing a bio-based hydrophobic nano-silica carrying fluid without biological enzyme, sequentially weighing 0.3 g of rhamnolipid, 0.5 g of propanol, 0.3 g of butanol, 0.68 g of amyl alcohol and 0.02 g of xanthan gum, dissolving in 98.2 g of water, stirring for 30 minutes at 100 revolutions per minute, heating to 40 ℃, preserving heat for 2 hours to obtain a comparative carrying fluid 1, then adding 0.1 g of hydrophobic nano-silicone material, stirring for 30 minutes at 120 revolutions per minute, and cooling for later use.

As can be seen in FIG. 4, the solution of example 1 was clear with no precipitation and no flocs present; the comparative carrier fluid 1 prepared in comparative example 2 was turbid and flocculated.

The average particle size of the nanoparticles in the fresh carrier fluid prepared in this comparative example was determined by a laser particle sizer (Mastersizer 3000). The average particle size of the nanoparticles in the nano-carrier fluid of example 1 was found to be 35.63nm, and the average particle size of the nanoparticles in the bio-based nano-carrier fluid without bio-enzyme was found to be 268.71nm. Example 1 the nano-dispersion in the nano-carrying fluid is superior to that of the carrying fluid prepared in comparative example 2. Has better dispersion stability.

Comparative example 3

Preparing a comparison carrier fluid 2 by using alpha-olefin sodium sulfonate and a sodium citrate surfactant, sequentially weighing 1.2 g of alpha-olefin sodium sulfonate and 0.8 g of sodium citrate, dissolving the alpha-olefin sodium sulfonate and the sodium citrate in 98 g of water, stirring the mixture for 30 minutes at 100 revolutions per minute, heating the mixture to 40 ℃, preserving the heat for 2 hours, then adding 0.1 g of hydrophobic nano polysilicon material, stirring the mixture for 30 minutes at 120 revolutions per minute, and cooling the mixture for later use.

As can be seen in FIG. 5, the solution of example 1 was clear with no precipitation and no flocs present; the comparative carrying fluid 2 has no turbidity than the clear substance.

The average particle size of the nanoparticles in the fresh carrier fluid prepared in this comparative example was determined by a laser particle sizer (Mastersizer 3000). The average particle size of the nanoparticles of the nano-carrier fluid of example 1 was measured to be 35.63nm, and the average particle size of the nanoparticles of the comparative carrier fluid 2 was measured to be 69.26nm. The comparative example results show that the biological-based nano-carrying fluid system is superior to the chemical-based nano-dispersion system in terms of the dispersion of the nano-particles.

Comparative example 4

This comparative example differs from example 1 only in that no microbial polysaccharide is involved, other raw materials and preparation process are the same as example 1;

comparative example 5

The comparative example is different from example 1 only in that the biosurfactant is not involved, and other raw materials and preparation processes are the same as those of example 1;

test example 1

The products prepared in example 1, comparative example 4 and comparative example 5 were subjected to a laboratory core displacement experiment comprising the following steps:

(1) Selecting an artificial core with a proper size, drying at 120 ℃, weighing the dry weight, measuring the diameter d and the length L of the artificial core, and calculating the apparent volume. Placing the mixture in simulated formation water (the degree of mineralization is 20000 mg/L) for saturation treatment for 12h.

(2) And putting the core into a core holder, performing ring pressing, and vacuumizing for 4h. Opening the oven, setting the temperature to 60 ℃ (the formation temperature), saturating and simulating formation water/clean water displacement to reach the displacement stable pressure, and testing the water drive pressure delta P under a certain flow ₁ The pore volume of the core is measured, the porosity is calculated, and the core permeability k is calculated according to the Darcy formula. Ions in the simulated formation water/clear water are uniformly distributed in the core.

(3) With KCl (3%) or NH ₄ Cl (3%) displacement is carried out for a period of time to reach the displacement stable pressure, the increment liquid with preset concentration and volume (2 PV to 5 PV) is injected at a certain flow (0.5 mL/min to 1 mL/min), and then the gate valve is closed at a set temperature and kept stand for more than 24 hours at a constant temperature;

(4) Cleaning the pipeline with clear water, and washing with KCl (3%) or NH ₄ Cl (3%) for a period of time, then displacing (1-3 PV) with formation water/clear water until no nano-liquid is present in the displaced fluid, and then testing the formation water-driving pressure delta P under a certain flow ₂ Calculating the permeability k of the core _h 。

By the formula: permeability improvement = (k) _h K)/k, and obtaining the rock core permeability improvement rate before and after the nano liquid flooding. The test adopts a single-point test method, the flow is required to be the same every time, and the recorded pressure value is stable.

The injection pressure reduction rate of simulated formation water before and after injection of the injection-increasing liquid in the embodiment 1 is obviously higher than that of the injection-increasing liquid in the comparative examples 4 and 5; after the injection of the injection-increasing liquid, the injection pressure of the clean water is obviously reduced (the injection pressure changes are shown in the table) in the embodiment 1, and the effect is better than that of the comparative examples 4 and 5.

TABLE 1

Test example 2

Wetting inversion Capacity test for Carrier fluid

In the test example, the core is a natural core, which is cut into a circular (D =5 cm) core slice with a thickness of 10-20mm by a special cutting machine, cleaned, and dried for later use.

Taking three sheets of the core tablet, placing one sheet in 50ml of the bio-based hydrophobic nano carrying fluid, placing one sheet in 50ml of kerosene, placing one sheet in 50ml of distilled water, and soaking in a water bath at 80 ℃ for 24 hours. The core slices were then removed, rinsed 3-5 times with clear water and dried for determination of the wetting angle (German OAC 200). The test results showed that the contact angle of the core tablet soaked with the bio-based hydrophobic nano carrier fluid system was 135 degrees (middle panel in fig. 2), the contact angle of the core tablet soaked with kerosene was 130 degrees (right panel in fig. 2), and the contact angle of the core tablet soaked with distilled water was 15 degrees (left panel in fig. 2). From fig. 2, it can be seen that the hydrophobic nanoparticles in the bio-based hydrophobic nano carrier fluid can be deposited on the rock surface to form a stable hydrophobic film, so that the change of the rock surface wettability from hydrophilic to hydrophobic is realized, and the water flow resistance of the rock surface is reduced. The hydrophobic effect is equivalent to that of kerosene.

Test example 3

Environmental testing of carrier fluids

The carrier fluids prepared in the example 1 and the comparative examples 1 and 3 are selected, and the final degradation rate of the carrier fluid is measured according to the anaerobic degradation test method GB/T27857-2011 of organic matters in China standard, and the degradation rate of the bio-based nano carrier fluid is measured under the laboratory condition. Organic carbon analysis is determined primarily by a Total Organic Carbon (TOC) determinator. The main process is to inoculate 1% activated sludge sample (from offshore oil field sewage treatment plant) in 1L of nano-carrier fluid. Aerobic shake flask culture, accurately transferring 50mL of culture solution into a centrifuge tube every 12h, centrifuging for 10min under the condition of 5000r/rain, then filtering the supernatant by using an aqueous filter head, storing in a sample bottle, and storing at-20 ℃ for TOC determination.

Rate of degradation

( C0 total organic carbon in the original nano carrying fluid; total organic carbon measured after degradation of Cn nano carrying fluid )

TABLE 2 microbial degradation ratio (%)

Sample(s)	Original (original)	12h	24h	36h	48h	60h	72h
								Example 1	0	18.72	45.28	56.78	78.26	92.93	97.37
Comparative example 1	0	0.52	1.31	1.35	2.11	2.08	2.56
								Comparative example 3	0	2.14	2.52	2.65	9.16	12.74	16.29

As can be seen from Table 2, the degradation rate of the bio-based nano-carrying fluid reaches 97.37% after 72 hours under the action of the microorganisms, and the fact that the bio-based nano-carrying fluid has the environmental protection characteristic and can be effectively degraded by the microorganisms is verified.

Although the embodiments disclosed in the present application are described above, the descriptions are only for the convenience of understanding the present application, and are not intended to limit the present application. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the disclosure as defined by the appended claims.

Claims

1. A nano silicon material carrying fluid comprises the following components in percentage by weight: 0.1 to 1 percent of biosurfactant, 0.1 to 2 percent of biological enzyme, 0.5 to 2 percent of cosolvent, 0.01 to 0.1 percent of microbial polysaccharide and the balance of water;

the biosurfactant is selected from any one or more of rhamnolipids, lipopeptides, sterols, lipoproteins and sophorolipids;

the biological enzyme is lipase, protease, esterase, isomerase and amylase which are combined according to the weight ratio of (0.01 to 0.1): 0.01 to 1): 0.05 to 0.5): 0 to 0.5: (0 to 0.5);

the lipase is selected from any one or two of lipase produced by microorganisms of pseudomonas and lipase produced by microorganisms of bacillus;

the protease is selected from any one or two of protease produced by bacillus microorganisms and protease produced by brevibacterium microorganisms;

the esterase is selected from one or two of microorganism esterase of Acinetobacter and microorganism esterase of Pseudomonas;

the isomerase is selected from any one or two of microorganism isomerase of the genus rhodobacter and microorganism isomerase of the genus acidophilic lactobacillus;

the amylase is selected from any one or two of amylase produced by bacillus microorganisms and amylase produced by halomonas microorganisms;

the cosolvent is selected from any one or more of propanol, isopropanol, butanol, pentanol and isoamyl alcohol;

the microbial polysaccharide is selected from one or two of lactobacillus microbe and xanthomonas microbe produced biomacromolecule microbial polysaccharide;

the microbial polysaccharide is extracellular polymer produced by microorganisms.

2. The carrier fluid of nano-silicone material as set forth in claim 1 wherein the microbial polysaccharide is xanthan gum.

3. The carrier fluid of nano-silicon material as claimed in any one of claims 1 to 2, wherein the nano-silicon material is a hydrophobic nano-polysilicon material.

4. The carrier fluid of nano-silicon material as claimed in claim 1, wherein the nano-silicon material is selected from any one or more of tert-butyl silane, vinyl silane, amino silane and siloxane coupling agent modified silicon material.

5. The method for preparing a nano silicon material carrier fluid according to any one of claims 1 to 4, the method comprising: stirring the raw materials uniformly, heating to 25-40 ℃, and preserving heat for 0-2h; cooling to obtain the nano silicon material carrying fluid.

6. The method for preparing the nano silicon material carrier fluid according to claim 5, wherein the rotation speed of the stirring is 100-180 r/min, and the stirring time is 10-60 min.

7. The method for preparing a nano silicon material carrier fluid according to claim 5 or 6, wherein the raw materials are added into the container in the order of water, biosurfactant, biological enzyme, cosolvent and microbial polysaccharide.

8. Use of the carrier fluid according to any one of claims 1 to 4 for stimulation of a water injection well.