CN112892677A

CN112892677A - Tissue homogenate grinding rod

Info

Publication number: CN112892677A
Application number: CN202110227276.3A
Authority: CN
Inventors: 翁长仁; 林炜明
Original assignee: Longyan University
Current assignee: Longyan University
Priority date: 2021-03-01
Filing date: 2021-03-01
Publication date: 2021-06-04

Abstract

The utility model provides a tissue homogenate grinding rod, including grinding head and grinding handle, the grinding handle is located the top of grinding head, the grinding head is moulded plastics or thermoforming after mixing by plastics and high rigidity powder again and is formed. Compared with the prior art that the grinding rod for the biological homogenate tissue is made of metal, glass or plastic such as polypropylene, the friction force between the grinding rod and the biological tissue is effectively increased, and the grinding effect is greatly improved.

Description

Tissue homogenate grinding rod

Technical Field

The disclosure relates to the field of biochemical instruments, in particular to a tissue homogenate grinding rod.

Background

At present, the material of the grinding rod of the biological tissue homogenizer on the market generally adopts metal, glass or plastics such as polypropylene, and the grinding rods have the following defects: the surface friction is insufficient and the grinding effect is poor. Fine powder such as carborundum, quartz sand, corundum and the like with high hardness and sharp edges and corners can be used for polishing the surfaces of metals and the like and can also be used for enhancing the grinding effect in the grinding process, for example, when a mortar and a grinding rod are used for grinding biological tissues, the carborundum is added into the biological tissues in the mortar to improve the grinding effect, but the carborundum is separated subsequently, so that the separation difficulty is increased. In view of the above, the present inventors have designed and proposed a tissue homogenizing and grinding rod.

Disclosure of Invention

The present disclosure provides a tissue homogenate grinding rod, which is manufactured by mixing plastic and fine powder with high hardness and sharp edges and corners, and then performing injection molding or film rolling molding, etc., so as to improve the grinding effect on biological tissue materials.

According to one aspect of the disclosure, the tissue homogenizing and grinding rod comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing plastic and high-hardness powder and then performing injection molding or thermal forming.

According to at least one embodiment of the present disclosure, the weight ratio of the plastic to the high hardness powder is 1: (0.05-3).

According to at least one embodiment of the present disclosure, the high hardness powder is a high hardness powder having a mohs hardness of between 6 and 10.

According to at least one embodiment of the present disclosure, the high hardness powder is one or more of quartz, boron carbide, alumina, corundum, carborundum, topaz, or diamond.

According to at least one embodiment of the present disclosure, the high hardness powder has a particle size of 20 to 1000 mesh.

According to at least one embodiment of the present disclosure, the plastic is one or more of polypropylene, polyethylene, polyvinyl chloride, polychloroprene, Polystyrene (PS), polystyrene-co-acrylonitrile, acrylonitrile-butadiene-co-styrene (ABS), polymethacrylate, ethylene-co-vinyl acetate, polyethylene terephthalate, polybutylene terephthalate, polyamide, polycarbonate, polyoxymethylene resin, polyphenylene oxide, polyphenylene sulfide, polyurethane, polystyrene, polytetrafluoroethylene.

According to at least one embodiment of the present disclosure, the shape of the abrading head is adapted to the bottom of the container to be abraded.

According to at least one embodiment of the present disclosure, the container to be ground comprises a commercially available 0.1 ml, 0.2 ml PCR reaction tube, 0.1 ml, 0.2 ml eight-row PCR tube, 0.1 ml, 0.2 ml 96-well plate, 1.5 ml, 2 ml, 5 ml, 10 ml, 15 ml, 50 ml plastic centrifuge tube.

According to at least one embodiment of the present disclosure, the polishing handle and the polishing head are formed by integral injection molding, integral thermoforming or integral film rolling.

According to at least one embodiment of the present disclosure, the grinding handle is formed by mixing plastic with high hardness powder and then injection molding or thermoforming.

After adopting above-mentioned technical scheme, this disclosure has following beneficial effect: compared with the prior art that the grinding rod for the biological homogenate tissue is made of metal, glass or plastic such as polypropylene, the friction force between the grinding rod and the biological tissue is effectively increased, and the grinding effect is greatly improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the disclosure and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the disclosure and together with the description serve to explain the principles of the disclosure.

FIG. 1 is a lapping rod of the present disclosure mated to 0.1 and 0.2 ml eight-row PCR tubes;

FIG. 2 is a grinding bar of the present disclosure mated to a 1.5 ml plastic centrifuge tube;

FIG. 3 is a grinding bar of the present disclosure mated to a 2 ml plastic centrifuge tube;

FIG. 4 is a grinding bar of the present disclosure mated to a 15 ml conical bottom centrifuge tube;

FIG. 5 is a grinding rod of the present disclosure mated to a 50 ml conical bottom centrifuge tube.

Detailed Description

The present disclosure will be described in further detail with reference to the drawings and embodiments. It is to be understood that the specific embodiments described herein are for purposes of illustration only and are not to be construed as limitations of the present disclosure. It should be further noted that, for the convenience of description, only the portions relevant to the present disclosure are shown in the drawings.

It should be noted that the embodiments and features of the embodiments in the present disclosure may be combined with each other without conflict. The present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

Example 1

The embodiment 1 provides a tissue homogenizing and grinding rod, which comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing polypropylene and quartz powder and then performing injection molding; the granularity of the quartz powder is 20 meshes. The injection molded lapping rod shape was matched to 0.1 and 0.2 ml eight-row PCR tubes, and the schematic shape is shown in FIG. 1. As a preferable mode of this embodiment 1, the ratio of polypropylene to quartz powder is 1: 0.05.

example 2

The embodiment 2 provides a tissue homogenizing and grinding rod, which comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing polyethylene and corundum powder and then performing hot forming; the granularity of the corundum powder is 500 meshes. The thermoformed grinding rod is matched with a 1.5 ml plastic centrifuge tube in shape, and the schematic diagram of the shape is shown in FIG. 2. As a preferable mode of this example 2, the ratio of polyethylene to corundum powder by weight is 1: 0.1.

example 3

The embodiment 3 provides a tissue homogenate grinding rod, which comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing PS (polystyrene) and diamond powder and then performing injection molding; the granularity of the diamond powder is 1000 meshes. The shape of the injection-molded grinding rod is matched with a 2 ml plastic centrifuge tube, and the schematic shape is shown in figure 3. As a preferable mode of this embodiment 3, the ratio by weight of PS to diamond powder is 1: 0.5.

example 4

The embodiment 4 provides a tissue homogenizing and grinding rod, which comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing ABS and quartz powder and then performing hot forming; the granularity of the quartz powder is 500 meshes. The thermoformed grinding rod shape was matched to a 15 ml conical bottom centrifuge tube, and a schematic shape is shown in fig. 4. As a preferable mode of this embodiment 4, ABS and silica powder are mixed in a weight ratio of 1: 0.8.

example 5

The embodiment 5 provides a tissue homogenizing and grinding rod, which includes a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing polyurethane and boron carbide powder and then performing injection molding; the granularity of the boron carbide powder is 500 meshes. The injection molded grinding rod is matched with a 50 ml conical-bottom centrifuge tube in shape, and the schematic diagram of the shape is shown in FIG. 5. As a preferable mode of this example 5, the ratio of polyurethane to boron carbide powder by weight is 1: 2.

example 6

The embodiment 6 provides a tissue homogenizing and grinding rod, which comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing PS and topaz powder and then performing hot forming; the granularity of the yellow jade powder is 500 meshes. The thermoformed grinding rod shape was matched to a 50 ml conical bottom centrifuge tube, and a schematic of the shape is shown in FIG. 5. As a preferable mode of this embodiment 6, the weight ratio of PS to topaz powder is 1: 3.

as a preferred embodiment of examples 1-6, the polishing shanks of examples 1-6 were injection molded or thermoformed integrally with the polishing heads of examples 1-6, respectively.

Comparative example 1

The tissue homogenizing grinding rod comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by thermal forming of ABS. The thermoformed grinding rod shape was matched to a 15 ml conical bottom centrifuge tube, and a schematic shape is shown in fig. 4.

Comparative example 2

The tissue homogenizing grinding rod comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by glass through thermal forming. The thermoformed grinding rod shape was matched to a 15 ml conical bottom centrifuge tube, and a schematic shape is shown in fig. 4.

Comparative example 3

The tissue homogenizing grinding rod comprises a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by hot forming of metal iron. The thermoformed grinding rod shape was matched to a 15 ml conical bottom centrifuge tube, and a schematic of the shape is shown in FIG. 4.

The tissue homogenizing and grinding rods of examples 4 to 6 and comparative examples 1 to 3 were subjected to grinding effect tests, and 5g of skin tissue samples were ground for 20min by the respective grinding rods, and the results are shown in table 1 below.

TABLE 1 grinding Effect test results

Group of	Range of particle size distribution
		Example 4	0.5-0.9mm
Example 5	0.4-0.8mm
		Example 6	0.3-0.7mm
Comparative example 1	2.4-5.8mm
		Comparative example 2	2.2-5.2mm
Comparative example 3	2.3-4.8mm

As can be seen from Table 1, examples 4-6 of the present disclosure have a particle size distribution ranging between 0.3-0.9mm with a maximum value of less than 1 mm; while comparative examples 1-3 have a particle size distribution in the range of 2.2-5.8mm with a minimum value greater than 2.2 mm. From this, it is understood that the polishing head added with the high hardness powder greatly increases the polishing effect of the biological tissue sample. Of course, the high hardness powder of the present disclosure may be natural mineral powder or synthetic mineral powder.

The grinding effect is greatly improved, so that the components in the tissue cells to be ground are released more fully, and the extraction rate of components such as nucleic acid, protein, saccharides and the like of the cell tissues is greatly improved. In order to increase the extraction, it is common practice to increase the number of samples, which is difficult for some scarce samples. The present disclosure can obtain a certain amount of cell components even in a limited sample by improving the extraction rate, and meet the subsequent scientific research or production requirements.

The shape of the grinding head disclosed by the invention is closely matched with the bottom of the container according to the bottom design of the matched container, the grinding head can extend into containers such as a centrifuge tube and the like, the grinding handle can be inserted into the electric motor for fixing and screwing, and the grinding head rubs with the bottom of the container under the driving of the rotation of the motor or the assistance of manpower through the grinding handle to grind biological tissues.

The tissue homogenate grinding rod disclosed by the invention has a good grinding effect, can be used for efficiently grinding cell tissues in a test tube and a centrifuge tube, and is used for extracting components such as cell tissue nucleic acid, protein and saccharides; the grinding rod can rub the bottom and the lower side surface of a container such as a centrifuge tube and the like to break biological materials such as cell tissues and the like with the assistance of manpower, and can also be arranged on an instrument with an automatic rotating arm to grind the biological materials such as the broken cell tissues and the like, so that the automation of extraction is realized.

In the description herein, reference to the description of the terms "one embodiment/mode," "some embodiments/modes," "example," "specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment/mode or example is included in at least one embodiment/mode or example of the application. In this specification, the schematic representations of the terms used above are not necessarily intended to be the same embodiment/mode or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments/modes or examples. Furthermore, the various embodiments/aspects or examples and features of the various embodiments/aspects or examples described in this specification can be combined and combined by one skilled in the art without conflicting therewith.

It will be understood by those skilled in the art that the foregoing embodiments are merely for clarity of illustration of the disclosure and are not intended to limit the scope of the disclosure. Other variations or modifications may occur to those skilled in the art, based on the foregoing disclosure, and are still within the scope of the present disclosure.

Claims

1. The tissue homogenizing grinding rod is characterized by comprising a grinding head and a grinding handle, wherein the grinding handle is arranged above the grinding head, and the grinding head is formed by mixing plastic and high-hardness powder and then performing injection molding or thermal forming.

2. The tissue homogenizing and grinding rod according to claim 1, wherein the weight ratio of the plastic to the high hardness powder is 1: (0.05-3).

3. The tissue homogenizing mill rod of claim 1, wherein the high hardness powder is a powder having a mohs hardness between 6 and 10.

4. The tissue homogenizing and grinding rod according to claim 3, wherein the high hardness powder is one or more of quartz, boron carbide, alumina, corundum, carborundum, topaz or diamond.

5. The tissue homogenizing mill rod of claim 1, wherein the high hardness powder has a particle size of 20-1000 mesh.

6. The tissue homogenizing mill rod of claim 1, wherein the plastic is one or more of polypropylene, polyethylene, polyvinyl chloride, polychloropropene, polystyrene-co-styrene-acrylonitrile, acrylonitrile-co-butadiene-co-styrene, polymethacrylate, ethylene-co-vinyl acetate, polyethylene terephthalate, polybutylene terephthalate, polyamide, polycarbonate, polyoxymethylene resin, polyphenylene oxide, polyphenylene sulfide, polyurethane, polystyrene, polytetrafluoroethylene.

7. The tissue homogenizing and grinding rod of claim 1, wherein the grinding head is shaped to fit the bottom of the container to be ground.

8. The tissue homogenizing and grinding rod of claim 7, wherein the containers to be ground comprise commercially available 0.1 ml, 0.2 ml PCR reaction tubes, 0.1 ml, 0.2 ml eight-row PCR tubes, 0.1 ml, 0.2 ml 96-well plates, 1.5 ml, 2 ml, 5 ml, 10 ml, 15 ml, 50 ml plastic centrifuge tubes.

9. The tissue homogenizing and grinding rod of claim 1, wherein the grinding handle and the grinding head are formed by injection molding or thermoforming.

10. The tissue homogenizing and grinding rod according to claim 1, wherein the grinding handle is formed by mixing plastic with high hardness powder and then injection molding or thermoforming.