CN112891399A - Traditional Chinese medicine composition and application and preparation method thereof - Google Patents

Traditional Chinese medicine composition and application and preparation method thereof Download PDF

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CN112891399A
CN112891399A CN201911130538.3A CN201911130538A CN112891399A CN 112891399 A CN112891399 A CN 112891399A CN 201911130538 A CN201911130538 A CN 201911130538A CN 112891399 A CN112891399 A CN 112891399A
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parts
traditional chinese
chinese medicine
medicine composition
cistanche
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雷家荃
雷克晟
刘建文
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/64—Orobanchaceae (Broom-rape family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction

Abstract

The invention provides a traditional Chinese medicine composition for eliminating human fatigue and improving and recovering the health state, which is prepared from three traditional Chinese medicines of cistanche, cinnamon and liquorice, and is combined together by different methods for recovering the fatigue and the sub-health state of the body. The Chinese medicinal composition can eliminate fatigue of human body and improve functional state of human body.

Description

Traditional Chinese medicine composition and application and preparation method thereof
Technical Field
The invention provides a traditional Chinese medicine composition for eliminating human fatigue and improving and recovering physical health state, and an application and a preparation method thereof, belonging to the technical field of traditional Chinese medicine and pharmacy and pharmaceutical industry.
Background
In recent years, fatigue, also called fatigue, is a subjective and powerless discomfort, and feeling fatigue is not a specific symptom. In the high-speed, high-efficiency and tense life of people, many people keep busy without stopping like the clockwork spring. In the past for a long time, the body of a person is general weakness, listlessness, poor spirit, tired and slow, so the body is in a fatigue state. Another manifestation of fatigue is that a person in "sub-health" state of fatigue and sub-health, if left to develop, may turn into disease.
The modern Chinese medicine of the traditional Chinese medicine considers that fatigue is a disease name, has etiology and pathogenesis, is a common disease and frequently encountered disease in clinic, is a new disease category which must be regarded as important, belongs to the sub-health category, relates to internal organs, and mainly takes spleen, liver and kidney as main components. Can be regulated and treated by the traditional Chinese medicine syndrome differentiation formula to achieve the purposes of prevention, treatment and control. The traditional Chinese medicine considers that fatigue is caused by the deficiency syndrome of primordial qi consumption and the psychological change (or disorder). In treatment, it is necessary not only to tonify deficiency and strengthen body resistance, but also to dispel fatigue and induce tranquilization. On one hand, the traditional Chinese medicine composition can tonify deficiency and consolidate vitality, nourish the body, tonify deficiency, strengthen healthy qi and remove fatigue; on the other hand, it can regulate heart, soothe heart, refresh mind, calm mind, and relieve fatigue.
The inventor of the present invention is a ninth generation ancestor on the basis of inheriting the ancestral empirical formula, and finds that the traditional Chinese medicine mainly comprising cistanche deserticola is processed to eliminate the fatigue state of the human body, and has very significant effect.
Cistanchis herba (Cistanche deserviola Ma) is named as Jiangxi, Cistanchis herba, and Chazhuan Yam (Mongolian language), and belongs to endangered species. Big and tall herbs, 40-160 cm tall, mostly grown underground. The flowering period is 5-6 months, and the fruit period is 6-8 months. Mainly produced in Sinkiang and the inner Mongolia AlaLau, and also distributed in Gansu and Ningxia. Cistanche salsa is a parasitic plant parasitic on the root of haloxylon ammodendron in desert trees, and absorbs nutrients and water from haloxylon ammodendron hosts. It is known as "desert ginseng".
Cinnamon, cinnamon (the Latin scientific name: Cinnamomum cassia Presl), also known as cinnamon, oyster, caju, cinnamon, Pink cinnamon, Chinese cinnamon, is the dry bark of cinnamon of Lauraceae.
Licorice, (academic name: Glycyrrhiza uralensis Fisch) another name: radix Angelicae Dahuricae, radix Glycyrrhizae, and radix Glycyrrhizae. Leguminosae and liquorice belong to perennial herbs, and the roots and the rhizomes are thick and strong, so that the Chinese herbal medicine is a tonifying Chinese herbal medicine. The medicinal parts of the traditional Chinese medicine are roots and rhizomes, and the medicinal root is cylindrical, 25-100 cm long and 0.6-3.5 cm in diameter. The skin has different elasticity, and the surface is reddish brown or grayish brown. The root and stem are cylindrical, the surface has bud mark, and the middle of the section has marrow. Light smell, sweet and special taste.
The inventors have surprisingly found that the composition consisting of cistanche, cinnamon and licorice is very effective in eliminating fatigue of the human body and improving and restoring the health state of the human body.
According to the understanding of the inventor, at present, no composition consisting of cistanche, cinnamon and licorice is very effective in eliminating fatigue of a human body and improving and restoring a healthy state of the human body.
Disclosure of Invention
The invention aims to provide an effective traditional Chinese medicine composition for eliminating human fatigue and improving and recovering physical health state, which is used for recovering body fatigue and sub-health state. The Chinese medicinal composition comprises Cistanchis herba, cortex Cinnamomi and Glycyrrhrizae radix. According to the weight ratio, the cistanche deserticola can be 0-60 parts, the cinnamon can be 0-6 parts, and the liquorice can be 0-10 parts; suitably, 5-50 parts of cistanche, 0.5-5.5 parts of cinnamon and 1-9 parts of liquorice; more suitably, 15-40 parts of cistanche, 1.5-4.5 parts of cinnamon and 3-7 parts of liquorice; preferably, 20-35 parts of cistanche, 2-4 parts of cinnamon and 4-6 parts of liquorice; most preferably, 30 parts of cistanche, 3 parts of cinnamon and 5 parts of liquorice.
In one aspect of the present invention, there is provided a method of preparing the composition of the present invention, the method comprising: adding Cistanchis herba in water decoction, adding cortex Cinnamomi directly in non-heated powder state, and adding Glycyrrhrizae radix in unprocessed water decoction.
In a further aspect of the invention, the invention provides a preparation process and steps for preparing the pharmaceutical composition of the invention into a water decoction, wherein the raw medicinal materials are as follows: cinnamon: the liquorice is weighed according to the proportion. Mixing Cistanchis herba and Glycyrrhrizae radix, soaking in water for 30min, heating to boil, and decocting with slow fire for 30 min. Pouring out the medicinal liquid, filtering with gauze, heating and concentrating to decoct the medicinal liquid, adding weighed cortex Cinnamomi powder, stirring, and storing in refrigerator at 4 deg.C.
The significant advantages and technological advances of the present invention: the invention provides a medicine which is prepared from a composition, resists fatigue, improves exercise endurance and has bidirectional regulation. The medicine composition firstly overcomes the defect that the traditional Chinese medicine composition is only decocted in water. The method of the invention has simple operation and low production cost, and is suitable for large-scale industrial production. The combination provides an efficient, cost effective, patient burden reducing, does not contain any harmful substances, and does not pollute the environment. Has good prospect for treating fatigue diseases.
The specific implementation method comprises the following steps:
certain aspects, modes, embodiments, variations and features of the present invention are described below in varying degrees of detail in order to provide a substantial understanding of the present invention.
In one embodiment of the present invention, the candidate Chinese herbal medicine composition for eliminating fatigue of human body and improving and restoring health state is selected, and the candidate Chinese herbal medicine composition for the present invention may be at least one of the following Chinese herbal medicines. The Chinese herbal medicine can be selected from Bulbus Allii Fistulosi, herba Centipedae, herba Coriandri, ramulus et folium Tamaricis, herba Menthae, herba Ephedrae, ramulus Cinnamomi, Perillae herba, rhizoma Zingiberis recens, herba Moslae, herba Schizonepetae, radix Saposhnikoviae, Notopterygii rhizoma, radix Angelicae Dahuricae, herba asari, rhizoma Ligustici, fructus Xanthii, flos Magnoliae, fructus Arctii, periostracum Cicadae, folium Mori, flos Chrysanthemi, fructus Vitics Simplicifoliae, bupleuri radix, cimicifugae rhizoma, radix Puerariae, semen Sojae Preparatum, herba Spirodelae, herba Equiseti hiemalis, Gypsum Fibrosum, Mirabilitum crystallina, rhizoma anemarrhenae, rhizoma Phragmitis, Trichosanthis radix, folium Bambusae, herba Commelinae, fructus Gardeniae, Prunellae Spica, semen Cassiae, faeces Vespertilionis, flos Buddlejae, semen Celosiae, fel Zaocys, fel Sus Domestica, Scutellariae radix Coptidis rhizoma, cortex Phellodendri, radix Gentianae, cortex Fraxini, radix Sophorae Flavescentis, cortex Dictamni Radicis, herba Berberidis Amureae, rhizoma et radix Berberidis, cortex et rhizoma, Herba Houttuyniae, radix Et rhizoma Fagopyri Tatarici, caulis Sargentodoxae, herba Patriniae, rhizoma Belamcandae, radix Sophorae Tonkinensis, Lasiosphaera Seu Calvatia, fructus Canarii albi, calyx seu fructus physalis, radix tinosporae, semen Oroxyli, radix Pulsatillae, herba Portulacae, fructus Bruceae, herba Euphorbiae Humifusae, colla Plastri Testudinis, herba Potentillae chinensis, herba Potentillae Discoloris, herba Lobeliae chinensis, herba Hedyotidis Diffusae, Pseudobulbus Cremastrae seu pleiones, herba Senecionis Japonicae, rhizoma Polygonati, herba Apii Graveolentis, radix astragali, semen phaseoli radiati, rhizoma paridis, herba Verbenae Bidentis, radix Tripterygii Wilfordii, folium Hibisci Mutabilis, herba Scutellariae Barbatae, herba Acalyphae, fructus Canaricae, radix Ardisiae Crenatae, radix Achyranthis bidentatae, herba Pileae Scriptae, radix rehmanniae, cortex moutan, radix Paeoniae Rubra, radix Arnebiae, cornu Bubali, herba Rabdosiae Lophanthoidis, herba Artemisiae Annuae, cortex Lycii, radix Stephaniae Japonicae, radix Aconiti, colla Cornus Cervi, Agkistrodon, Zaocys, scarlet caterpiller fungus, fructus Chaenomelis, faeces bombycis, herba Lycopodii, herba Aristolochiae Mollissimae, lignum Pini nodi, caulis Piperis Futokadsurae, caulis Sinomenii, caulis Erycibes, Kunming begonia leaf, radix Aconiti Brachypodi, fructus Lipuidambaris, resina Liquidambaris, herba Saussureae Involueratae, radix Tripterygii Wilfordii, radix Cynanchi Paniculati, radix Lamii, flos Rhododendri mollis, radix Zanthoxyli, Chinese Alangium, radix Gentianae Marcrophyllae, radix Stephaniae Tetrandrae, Siegesbeckiae herba, folium et ramulus Clerodendri Trichotomi, cortex Erythrinae, herba Geranii, rhizoma homalomenae, herba Saussureae Involueratae, herba Pyrolae, folium Photiniae, semen plantaginis, aromatic herba Pogostemonis, herba Eupatorii, rhizoma Atractylodis, cortex Magnoliae officinalis, fructus Amomi rotundus, semen Alpiniae Katsumadai, semen Caulophyllae, fructus Tsaoko, Poria, Medulla Tetrapanacis, herba Dianthi, herba Polygoni Avicularis, Kochiae fructus, spora Lygodii, folium Pyrrosiae, semen Abutili, medulla Junci, rhizoma Dioscoreae Septemlobae, herba Artemisiae Scopariae, herba Lysimachiae Christinae, rhizoma Polygoni Cuspidati, herba Hyperici Japonici, herba Selaginellae Doederleinii, Ginseng radix, herba abri, herba Phyllanthi Urinariae, herba Centellae, radix Aconiti lateralis Preparata, Zingiberis rhizoma, cortex Cinnamomi, fructus evodiae, fructus Foeniculi, flos Caryophylli, rhizoma Alpiniae Officinarum, fructus Piperis, fructus Zanthoxyli, fructus Piperis Longi, fructus Litseae, rhizoma Kaempferiae pericarpium Citri Tangerinae, semen Citri Reticulatae, vascular Aurantii, folium Citri Tangerinae, exocarpium Citri Reticulatae viride, fructus Aurantii Immaturus, radix aucklandiae, lignum Aquilariae Resinatum, lignum Santali albi, fructus Toosendan, radix Linderae, radix Aristolochiae, semen litchi, rhizoma Cyperi, fructus Citri Sarcodactylis, fructus Citri, flos Rosae Rugosae, semen Phaseoli, semen Aesculi, fructus Toosendan, Bulbus Allii Macrostemonis, caulis et Gemma Agrimon, Fructus Carpesii, Torreya grandis, Ulmus Preparatum, herba Cephalanoploris, herba seu radix Cirsii Japonici, radix Sanguisorbae, flos Sophorae, cacumen Platycladi, lalang grass rhizome, radix Boehmeriae, radix Rumicis Japonici, Notoginseng radix, folium Artemisiae Argyi, rhizoma Zingiberis Preparata, furnace soil, Notoginseng radix, radix Rubiae, pollen Typhae, Ophicalcitum, lignum Dalbergiae Odoriferae, crinis Carbonisatus, rhizoma Bletillae, herba et Gemma Agrimoniae, folium Callicarpae Formosanae, pulvis Fumi Carbonisatus, petiolus Trachycarpi, nodus Nelumbinis Rhi: concha Haliotidis, Concha Margaritifera, Concha Ostreae, Concha Erosariae Seu Cypraeae, Haematitum, fructus Tribuli, herba Apocyni Veneti, pulvis Ferri, semen Sojae Atricolor, cornu Saigae Tataricae, calculus bovis, Margarita, ramulus Uncariae cum uncis, folium Ginkgo, rhizoma Gastrodiae, Lumbricus, Scorpio, Scolopendra, Bombyx Batryticatus, rhizoma Ligustici Chuanxiong, rhizoma corydalis, radix Curcumae, Curcuma rhizome, Olibanum, Myrrha, Oletum Trogopterori, rhizoma Acori Graminei, rhizoma corydalis Decumbentis, resina Liquidambaris, flos Impatientis, Saviae Miltiorrhizae radix, Carthami flos, semen Persicae, herba Leonuri, herba Lycopi, Achyranthis radix, caulis Spatholobi, semen Vaccariae, maca, flos Rosae chinensis, flos Campsis, Glycyrrhrizae radix, Eupolyphaga Seu Steleophaga, semen Strychni, Pyritum, lignum sappan, rhizoma arisaematis, Mylabris, sanguis Draxonis, Catechu, herba Artemisiae Anomalae, fructus Aristolochiae, fructus Gardeniae, semen Cuscutae, rhizoma Polygoni Multiflori radix Polygoni Multiflori, rhizoma Typhonii, semen Sin, Bulbus Fritillariae Cirrhosae, Bulbus Fritillariae Thunbergii, flos Hibisci, fructus Trichosanthis, caulis Bambusae in Taenia, succus Bambusae, concretio silicea Bambusae seu Schizostachyi, radix Peucedani, radix Platycodonis, semen Scaphii Lychnophori, Sargassum, thallus laminariae, rhizoma Dioscoreae Bulbiferae, Concha Meretricis Seu Cyclinae, Pumex, Concha arcae, lapis Micae aureus, fructus Jujubae, herba Rorippae, semen Armeniacae amarum, fructus Perillae, radix Stemonae, radix Asteris, flos Farfarae, fructus Aristolochiae, folium Eriobotryae, cortex Mori, semen Lepidii, semen Ginkgo, herba Ardisiae Japonicae, flos Daturae Metelis, radix Phyllanthi, fructus Siraitiae Grosvenorii, flos Rhododendri, folium Elaeagni, Cinnabaris, Magnetitum, Succinum, Margarita, semen. Those skilled in the art will appreciate that the Chinese herbal composition of the present invention can be selected according to the different experimental results (such as orthogonal experiment and/or animal experiment). The candidate traditional Chinese medicine composition suitable for eliminating human fatigue and improving and recovering the health state of the human body consists of astragalus, dogwood, monkshood, cinnamon, ligusticum wallichii, semen cuscutae, polygonum multiflorum, arisaema cum bile, erigeron breviscapus, rhizoma acori graminei, radix curcumae, salvia miltiorrhiza, earthworm, stiff silkworm, cistanche deserticola, radix ophiopogonis, poria cocos, rhizoma polygonati, deerhorn glue, tortoise plastron glue, liquorice, antelope horn, bezoar, pearl essence, dandelion and purslane.
The inventor combines the various traditional Chinese medicines in different proportions, designs a mouse weight swimming test according to an orthogonal design method, and performs an optimal combination test. By combining a negative control group (distilled water) and the traditional Chinese medicines in different proportions, and observing a load swimming test of a mouse after the mouse is filled with different combined medicines, the inventor surprisingly finds that the swimming time of the mouse is remarkably prolonged after the cistanche, the cinnamon and the liquorice are combined.
The inventors then performed gavage experiments on experimental clean male ICR mice with different combinations of the three monomeric components. The experimental results prove that the three different monomers have different functions on resisting fatigue of the mice in vivo, and the combination of the three monomers is found to obviously improve the fatigue symptom of the mice and improve the exercise endurance of the mice.
In one embodiment of the present invention, the ratio of each component in the most preferred Chinese medicinal composition suitable for relieving fatigue, improving and restoring health condition of human body is selected, in the most preferred Chinese medicinal composition, cistanche salsa may be 0-60 parts, for example, 0.1-60 parts, 0.3-60 parts, 0.5-60 parts, 0.6-60 parts, 0.7-60 parts, 0.8-60 parts, 0.9-60 parts, 1-60 parts, 2-60 parts, 3-60 parts, 4-60 parts, suitably 5-50 parts by weight; more suitably from 10 to 45 parts; preferably 15-40 parts; more preferably 20 to 35 parts; most preferably 30 parts.
In yet another embodiment of the present invention, the ratio of each component in the most preferred candidate Chinese medicinal composition suitable for relieving fatigue, improving and restoring physical health is selected, wherein the most preferred Chinese medicinal composition comprises 0 to 6 parts by weight of cinnamon, such as 0.1 to 6 parts by weight, 0.2 to 6 parts by weight, 0.3 to 6 parts by weight, 0.4 to 6 parts by weight, 0.5 to 6 parts by weight; suitably 0.5 to 5.5 parts; more suitably 1 to 5 parts; preferably 1.5-4.5 parts; more preferably 2-4 parts; most preferably 3 parts.
In still another embodiment of the present invention, the ratio of each component in the most preferred candidate Chinese medicinal composition suitable for relieving fatigue, improving and restoring physical health condition is selected, and in the most preferred candidate Chinese medicinal composition, licorice may be 0-10 parts, 0.1-10 parts, 0.2-10 parts, 0.3-10 parts, 0.4-10 parts, 0.5-10 parts, 0.6-10 parts, 0.7-10 parts, 0.8-10 parts by weight; 0.9-10 parts, 1-10 parts and 1-9 parts; suitably 1 to 8 parts; more suitably from 2 to 7 parts; preferably 3-7 parts; more preferably 4-6 parts; most preferably 5 parts.
The anti-fatigue composition is nontoxic and suitable for oral or parenteral administration to human and mammals. The anti-fatigue composition of the present invention is not particularly limited in dosage form, and can be used as solid preparations such as powder, pill, tablet, capsule and the like for oral administration. The composition having an anti-fatigue function of the present invention can be formulated into a preparation for oral or parenteral administration usually in combination with an auxiliary material which does not affect the pharmacological effect. The anti-fatigue composition of the present invention can be prepared by a conventional method using a solvent such as an aqueous solvent (e.g., distilled water), a water-soluble agent (e.g., physiological saline), a fat solvent (e.g., sesame oil), etc. The antifatigue composition of the present invention can be usually combined with pharmacologically acceptable additives (diluents, colorants, stabilizers, surfactants, solubilizers, buffers, flavors, perfumes, preservatives, sugar-coated agents) and used in oral or parenteral dosage forms. The fatigue-preventing composition of the present invention can be used in an oral dosage form, usually in combination with pharmacologically acceptable diluent components and excipient components.
In yet another embodiment of the invention, a prophylactically and/or therapeutically effective amount of an active peptide comprising a peptide prepared according to the invention is administered to a human daily, said effective amount may be any effective amount, such as 0.5ug/kg, 1ug/kg or 10ug/kg body weight of the human, suitably an effective amount comprises administration of about 1ug/kg to about 10000mg/kg body weight of the active peptide to the human daily.
The term "effective amount" as used herein refers to an amount sufficient to obtain a desired therapeutic and/or prophylactic effect, e.g., to cause prevention or alleviation of symptoms associated with a pathological condition or disease. The amount of the composition administered to a subject will depend on the type and severity of the disease and the nature of the individual, such as general health, age, sex, body weight and tolerance to drugs. The amount also depends on the extent, severity and type of the disease. One skilled in the art will be able to determine the appropriate dosage based on these and other factors. The composition may also be administered in combination with one or more other therapeutic compounds. In the methods described herein, the compounds of the invention may be administered to a subject having one or more signs of a pathological condition or a pathological condition. For example, a "therapeutically effective amount" refers to the average level of physiological effect that minimally alleviates a symptom.
In still another embodiment of the present invention, a process and steps for preparing a water decoction of the pharmaceutical composition of the present invention are provided, and the tested compound formula is cistanche, cinnamon and licorice. According to the raw medicinal materials of cistanche deserticola: meat and cassia twig: and weighing the liquorice. Mixing Cistanchis herba and Glycyrrhrizae radix, soaking in water for 30min, heating to boil, and decocting with slow fire for 30 min. Pouring out the medicinal liquid, filtering with gauze, heating and concentrating the medicinal liquid, adding weighed Cinnamomum cassia powder, stirring, and storing in refrigerator at 4 deg.C.
Typically, the dose will be capable of preventing or lessening the severity or extent of the condition or indication being treated. The exact dosage will depend on the circumstances, e.g., the condition being treated, the dosing schedule, whether the compound is administered alone or in combination with another therapeutic agent, the plasma half-life of the compound, and the overall health of the subject.
The invention will be better understood from the following description of examples, but is not limited to these examples.
Examples
Conditions adopted in Experimental examples 1 to 3
Grouping medicines:
the 3 monomer components were combined in orthogonal experimental groups using statistical principles. Each group of monomers is accurate in appearance, divided into full, half, and no three levels. According to L9(34) And designing an orthogonal table.
Orthogonal design table for fatigue resistance experiment (mass unit is g)
Figure BDA0002278169320000071
The high dose group (green part) and the low dose group (grey part) did not show what to compose the composition of cistanche, cinnamon and licorice from the three experiments, whether the three components are simple additive or synergistic, and it was not concluded from tables 1-3. Thus no conclusion of synergy is drawn. This is associated with the high inventive level of the present invention.
Weighing each group of the medicines according to the proportion shown in the table, soaking herba cistanches and radix Glycyrrhizae in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, and adding cortex Cinnamomi powder. (since the drug was ingested by gavage, it was dissolved in various solvents which may be toxic and then infused into the body of mice as a suspension.)
And taking mice fasted for 24 hours without water prohibition, randomly grouping, weighing and numbering. Then, each mouse is subjected to intragastric administration, the intragastric volume is 0.2ml, and the healthy group or the control group is infused with distilled water with the same volume. The time from swimming to death of the mice is recorded, wherein the time is 1 time per day, 20 days are continuously, 30min after the last administration of the liquid medicine, the mice are placed into water for swimming, the water depth is not lower than 30cm, the water temperature is 25 +/-1.0 ℃, the tail root of the mice is loaded with 10 percent of weight of lead blocks.
Orthogonal experimental result of fatigue resistance experiment
Figure BDA0002278169320000081
According to the basic idea of orthogonal experimental design:
Figure BDA0002278169320000082
the mean value corresponding to each level in the j-th column is shown. (
Figure BDA0002278169320000083
Represents the mean value of the weight swimming time corresponding to cistanche deserticola 30 in column 1;
Figure BDA0002278169320000084
represents the mean value of the weight swimming time corresponding to cistanche deserticola 15 in column 1;
Figure BDA0002278169320000085
represents the mean value of the weight swimming time corresponding to cistanche salsa 0 in column 1;
Figure BDA0002278169320000086
represents the mean value of the swimming time under load corresponding to cinnamon 3 in column 2;
Figure BDA0002278169320000087
represents the mean value of the swimming time under load corresponding to cinnamon 1.5 in column 2;
Figure BDA0002278169320000091
represents the mean value of the weight swimming time corresponding to cinnamon 0 in column 2;
Figure BDA0002278169320000092
represents the mean value of the weight swimming time corresponding to licorice 5 in column 3;
Figure BDA0002278169320000093
represents the mean value of the weight swimming time corresponding to licorice root 2.5 in column 3;
Figure BDA0002278169320000094
represents the mean value of the weight swimming time corresponding to licorice 0 in column 3;
Figure BDA0002278169320000095
the mean values of the swimming time under load corresponding to test numbers 1,5 and 9 in column 4 are shown;
Figure BDA0002278169320000096
the mean values of the swimming time with weight corresponding to test numbers 2,6,7 in column 4 are shown;
Figure BDA0002278169320000097
the mean values of the swimming time under load corresponding to test numbers 3,4 and 8 in column 4 are shown. )
Figure BDA0002278169320000098
The overall mean value of the weight swimming time is indicated.
SSjRepresenting the sum of squares of the j-th column. (SS)1Represents the sum of squares of column 1; SS2Represents the square sum of column 2; SS3Represents the sum of squares of column 3. )
SSTRepresents the overall square sum.
The analysis of variance table is listed, and significance test is performed (significance level α ═ 0.05):
Figure BDA0002278169320000099
from the above table data it can be derived:
because F1＝37.792>19.0＝F0.95(2,2), the effect of the cistanche is remarkable;
because F2＝1.888<19.0＝F0.95(2,2), so cinnamon action is not significant;
because F3＝0.932<19.0＝F0.95(2,2), the effect of licorice is not significant.
Combining the optimal levels of the cistanche, the cinnamon and the liquorice to obtain the optimal formula proportion of the cistanche: cinnamon: and (3) liquorice is 30:3:5 (by mass ratio).
Experimental example 1 mouse weight bearing swimming time
The single formula of the cistanche for testing is the cistanche. Weighing Cistanchis herba, soaking in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating and concentrating to obtain decoction containing crude drug 1g/ml, and storing in refrigerator at 4 deg.C.
The tested compound formula comprises cistanche, cinnamon and liquorice. According to the raw medicinal materials of cistanche deserticola: cinnamon: licorice root, 30:3:5g, was weighed. Mixing Cistanchis herba and Glycyrrhrizae radix, soaking in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating and concentrating to obtain water-decocted compound medicinal liquid containing crude drug 2.2g/ml, adding cortex Cinnamomi powder, and storing in refrigerator at 4 deg.C.
The experimental clean-grade male ICR mice, with a weight of 25-28 g, are provided by Shanghai Jitsie laboratory animal Limited company and have a production license number of SKXR (Shanghai) 2018-. The mice were randomly divided into 4 groups of 8 mice each, negative control group (distilled water), cistanche single group, cistanche compound low dose group and high dose group. By adopting the gavage method, the liquid medicine is orally administered, the gavage volume is 0.2ml, the negative control group is administered with distilled water with the same volume, and the low-dose compound group is administered with the compound liquid medicine diluted by one time with distilled water. 1 time per day for 20 days.
The drug is continuously administered for 20 days according to the dosage design, the mice are put into water for swimming 30min after the drug liquid is administered for the last time, the water depth is not lower than 30cm, the water temperature is 25 +/-1.0 ℃, the tail root of the mice is loaded with lead blocks with 10 percent of body weight, and the time from swimming to death of the mice is recorded.
The experimental results are shown in table 1 below. The results are statistically analyzed to find the high-dose cistanche compound
Table 1: mouse weight bearing swimming test
Figure BDA0002278169320000101
*And p is less than 0.05.
Experimental example 2 measurement of serum Urea Nitrogen in mouse
The single formula of the cistanche for testing is the cistanche. Weighing Cistanchis herba, soaking in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating and concentrating to obtain decoction containing crude drug 1g/ml, and storing in refrigerator at 4 deg.C.
The tested compound formula comprises cistanche, cinnamon and liquorice. According to the raw medicinal materials of cistanche deserticola: cinnamon: licorice root, 30:3:5g, was weighed. Mixing Cistanchis herba and Glycyrrhrizae radix, soaking in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating and concentrating to obtain water-decocted compound medicinal liquid containing crude drug 2.2g/ml, adding cortex Cinnamomi powder, and storing in refrigerator at 4 deg.C.
The experimental clean-grade male ICR mice, with a weight of 25-28 g, are provided by Shanghai Jitsie laboratory animal Limited company and have a production license number of SKXR (Shanghai) 2018-. The mice were randomly divided into 4 groups of 8 mice each, negative control group (distilled water), cistanche single group, cistanche compound low dose group and high dose group. By adopting the gavage method, the liquid medicine is orally administered, the gavage volume is 0.2ml, the negative control group is administered with distilled water with the same volume, and the low-dose compound group is administered with the compound liquid medicine diluted by one time with distilled water. 1 time per day for 20 days.
Continuously administering for 20 days according to dosage design, after the last administration of the medicinal liquid for 30min, putting the mouse into water at 25 + -1.0 deg.C, swimming for 90min without load, taking out, taking blood from eyeball after resting for 60min, centrifuging to obtain serum, and measuring the content of urea nitrogen in the serum according to the use procedure of a serum urea nitrogen measurement kit (Beijing Sorbao scientific and technology Co., Ltd.).
The results of the experiment are shown in table 2 below. Both the low dose and high dose groups reduced serum urea nitrogen content after exercise in the mice. Compared with a negative control group, the serum urea nitrogen content of the high-dose group is reduced by 23.49%; the serum urea nitrogen content of the low-dose group is reduced by 16.46 percent.
Table 2: determination of mouse serum urea nitrogen content
Figure BDA0002278169320000111
Figure BDA0002278169320000121
*And p is less than 0.05.
Experimental example 3 assay of liver glycogen content in mouse
The single formula of the cistanche for testing is the cistanche. Weighing Cistanchis herba, soaking in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating and concentrating to obtain decoction containing crude drug 1g/ml, and storing in refrigerator at 4 deg.C.
The tested compound formula comprises cistanche, cinnamon and liquorice. According to the raw medicinal materials of cistanche deserticola: cinnamon: licorice root, 30:3:5g, was weighed. Mixing Cistanchis herba and Glycyrrhrizae radix, soaking in water for 30min, boiling, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating and concentrating to obtain water-decocted compound medicinal liquid containing crude drug 2.2g/ml, adding cortex Cinnamomi powder, and storing in refrigerator at 4 deg.C.
The experimental clean-grade male ICR mice, with a weight of 25-28 g, are provided by Shanghai Jitsie laboratory animal Limited company and have a production license number of SKXR (Shanghai) 2018-. The mice were randomly divided into 4 groups of 8 mice each, negative control group (distilled water), cistanche single group, cistanche compound low dose group and high dose group. By adopting the gavage method, the liquid medicine is orally administered, the gavage volume is 0.2ml, the negative control group is administered with distilled water with the same volume, and the low-dose compound group is administered with the compound liquid medicine diluted by one time with distilled water. 1 time per day for 20 days.
The drug is administered for 20 days according to the dosage design, and the drug solution is sacrificed immediately 30min after the last administration. The liver was accurately weighed and then the liver glycogen content was determined according to the procedure of glycogen determination kit (Beijing Sorleibao science Co., Ltd.).
The results of the experiment are shown in table 3 below. The liver glycogen content of mice in the low-dose group and the high-dose group is higher than that of the negative control group, but the difference is not statistically significant (p > 0.05).
The experimental examples show that, in general, the cistanche compound traditional Chinese medicine liquid prolongs the weight swimming time of mice, reduces the content of serum urea nitrogen, and has particularly obvious effect in high-dose groups. In addition, the cistanche compound traditional Chinese medicine liquid can improve the liver glycogen storage capacity of mice and provide more energy for organisms so as to achieve the aim of resisting fatigue.
Table 3: determination of liver glycogen content in mice
Figure BDA0002278169320000131
The above embodiments and examples prove that the traditional Chinese medicine composition provided by the invention can eliminate fatigue of human body, improve and recover the health state of human body, and can be applied to recovery of body fatigue and sub-health state.
The present invention is not limited to the specific embodiments described in this application, but rather serves as a single illustration of separate aspects of the invention. Those skilled in the art will appreciate that various modifications and changes may be made without departing from the spirit and scope of the present application. Functionally equivalent uses within the scope of the disclosure, in addition to those enumerated herein, will be apparent to those skilled in the art from the foregoing description. Such changes and modifications are intended to fall within the scope of the appended claims. The present disclosure is to be limited only by the terms of the appended claims, along with the full scope of equivalents to which such claims are entitled. It is to be understood that this disclosure is not limited to particular methods, reagents, compositions, and biological systems, which can, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. All patents, patent applications, prior applications, and publications referred to or cited herein are incorporated by reference in their entirety so as not to conflict with the explicit teachings of this specification. Other embodiments are within the scope of the following claims.

Claims (10)

1. A traditional Chinese medicine composition is characterized in that: the traditional Chinese medicine composition consists of cistanche, cinnamon and liquorice.
2. The traditional Chinese medicine composition according to claim 1, characterized in that: in the traditional Chinese medicine composition, 0-60 parts of cistanche, 0-6 parts of cinnamon and 0-10 parts of liquorice are adopted.
3. The traditional Chinese medicine composition according to claim 2, characterized in that: in the traditional Chinese medicine composition, 5-50 parts of cistanche, 0.5-5.5 parts of cinnamon and 1-9 parts of liquorice are used.
4. The traditional Chinese medicine composition according to claim 3, wherein: in the traditional Chinese medicine composition, 15-40 parts of cistanche, 1.5-4.5 parts of cinnamon and 3-7 parts of liquorice.
5. The traditional Chinese medicine composition according to claim 4, wherein: in the traditional Chinese medicine composition, 20-35 parts of cistanche, 2-4 parts of cinnamon and 4-6 parts of liquorice are used.
6. The traditional Chinese medicine composition according to claim 5, wherein: in the traditional Chinese medicine composition, 30 parts of cistanche, 3 parts of cinnamon and 5 parts of liquorice are adopted.
7. The application of the traditional Chinese medicine composition is characterized in that: the use further comprises administering to a human a prophylactically and/or therapeutically effective amount of the traditional Chinese medicine composition of any one of claims 1 to 6.
8. Use according to claim 7, characterized in that: the effective amount comprises administering to a human a single formulation or composition of about 0.1mg/kg to about 10000mg/kg body weight per day.
9. A method of preparing a traditional Chinese medicine composition according to any one of claims 1 to 6, characterized in that: decocting herba cistanches with water, directly adding cortex Cinnamomi in non-heated powder state, and adding radix Glycyrrhizae in unprocessed water.
10. A method of preparing a traditional Chinese medicine composition according to any one of claims 1 to 6, characterized in that: according to the raw medicinal materials, the cistanche salsa: cinnamon: weighing Glycyrrhrizae radix, mixing Cistanchis herba and Glycyrrhrizae radix, soaking in water for 30min, heating to boil, decocting with slow fire for 30min, pouring out the medicinal liquid, filtering with gauze, heating to concentrate the medicinal liquid, adding weighed cortex Cinnamomi powder, stirring, and storing in refrigerator at 4 deg.C.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113208158A (en) * 2021-06-16 2021-08-06 吉林烟草工业有限责任公司 Chinese herbal medicine composition for improving cigarette aroma quality and cigarette
CN114344226A (en) * 2022-02-25 2022-04-15 河南科技大学 Traditional Chinese medicine preservative, preparation method and application
CN117018125A (en) * 2023-10-09 2023-11-10 吉林浩泰健康产业发展股份有限公司 Anti-fatigue pharmaceutical composition
CN117205122A (en) * 2023-10-13 2023-12-12 珍卡儿药妆有限公司 Aqueous antiseptic composition, preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1943545A (en) * 2006-10-17 2007-04-11 王海龙 Cosmetics composite and its preparing method and use
CN101152007A (en) * 2007-10-19 2008-04-02 金华寿仙谷药业有限公司 Health-care products composition and method for preparing the same
CN101322750A (en) * 2008-06-04 2008-12-17 张小平 Novel use of Cistanche tubulosa extract

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1943545A (en) * 2006-10-17 2007-04-11 王海龙 Cosmetics composite and its preparing method and use
CN101152007A (en) * 2007-10-19 2008-04-02 金华寿仙谷药业有限公司 Health-care products composition and method for preparing the same
CN101322750A (en) * 2008-06-04 2008-12-17 张小平 Novel use of Cistanche tubulosa extract

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
王志增: "甘草肉桂汤治疗低血压症的体会", 《中国社区医师》 *
石婷婷等: "肉苁蓉属植物的研究进展", 《中国民族民间医药》 *
韩晨霞: "基于线粒体生物合成探讨中枢疲劳的中医证候及生物学机制", 《中国优秀博硕士学位论文全文数据库(博士) 医药卫生科技辑》 *
韩树勤: "《调理药酒》", 31 July 2012, 农村读物出版社 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113208158A (en) * 2021-06-16 2021-08-06 吉林烟草工业有限责任公司 Chinese herbal medicine composition for improving cigarette aroma quality and cigarette
CN114344226A (en) * 2022-02-25 2022-04-15 河南科技大学 Traditional Chinese medicine preservative, preparation method and application
CN114344226B (en) * 2022-02-25 2023-03-17 河南科技大学 Traditional Chinese medicine preservative, preparation method and application
CN117018125A (en) * 2023-10-09 2023-11-10 吉林浩泰健康产业发展股份有限公司 Anti-fatigue pharmaceutical composition
CN117018125B (en) * 2023-10-09 2023-12-19 吉林浩泰健康产业发展股份有限公司 Anti-fatigue pharmaceutical composition
CN117205122A (en) * 2023-10-13 2023-12-12 珍卡儿药妆有限公司 Aqueous antiseptic composition, preparation method and application thereof

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