CN112891016A

CN112891016A - Biological 3D of controllable temperature low temperature prints shower nozzle device

Info

Publication number: CN112891016A
Application number: CN202110062280.9A
Authority: CN
Inventors: 刘天琪; 张祥林; 吴斌
Original assignee: Huazhong University of Science and Technology; Ezhou Institute of Industrial Technology Huazhong University of Science and Technology
Current assignee: Huazhong University of Science and Technology; Ezhou Institute of Industrial Technology Huazhong University of Science and Technology
Priority date: 2021-01-18
Filing date: 2021-01-18
Publication date: 2021-06-04
Anticipated expiration: 2041-01-18
Also published as: CN112891016B

Abstract

The utility model provides a biological 3D of controllable temperature low temperature prints shower nozzle device, includes: casing, installation piece, syringe subassembly, fixed subassembly, intake pipe, heating membrane, refrigeration piece and radiator unit. The temperature-controllable low-temperature biological 3D printing nozzle device provided by the application can adjust the temperature of a biological material as required, is not only beneficial to ensuring the performance consistency of the biological material, but also can be combined with an original low-temperature cooling platform to break through the layer height of low-temperature biological 3D printing, is beneficial to relaxing the requirements of a low-temperature 3D biological printing technology on the biological material, and enables more materials to have opportunities to be applied to the low-temperature biological 3D printing technology; meanwhile, the temperature-controllable spray head is adopted for low-temperature biological 3D printing, so that the scaffold which is higher in porosity and better in biocompatibility than the scaffold obtained by the original low-temperature biological 3D technology is expected to be obtained.

Description

Biological 3D of controllable temperature low temperature prints shower nozzle device

Technical Field

The invention belongs to the technical field of 3D printing, and particularly relates to a temperature-controllable low-temperature biological 3D printing nozzle device.

Background

In recent years, the auxiliary regeneration and repair of damaged parts by implanting various scaffolds made of biological materials through various 3D printing technologies is a hot spot in tissue engineering.

There are research papers that separate polymeric raw materials from various active materials and use multi-nozzle printing techniques to make scaffolds, but they use melt printing techniques. In some researches, a low-temperature printing technology is adopted, which is different from melting printing, and the low-temperature printing is solution printing without high-temperature melting. And because of low temperature, the sample has a rough porous surface appearance, is more favorable for the climbing growth of tissue cells, and has positive significance for promoting the rapid recovery of lesion tissues.

Meanwhile, low-temperature printing methods implemented by many patent papers adopt a low-temperature printing platform, and although the method is greatly improved in the aspects of performance and biocompatibility of a printing support compared with the original printing method, the method suffers from the temperature gradient of the low-temperature platform in the environment, so that the number of layers of the support cannot be too high in the printing process, and the printed wire diameter of a high layer is different from that of the wire diameter of a bottom layer. And often the equipment of the shower nozzle of printing with this kind of printing technique assorted is comparatively complicated, and the replaceability is poor, and the required cost of replacement is higher, can't carry out effectual accuse temperature to the shower nozzle, leads to printing the performance of thick liquids in the middle of the printing process and has the difference along with ambient temperature, causes the support of printing out finally to guarantee the performance, has restricted the development that biological 3D of low temperature printed.

Disclosure of Invention

In view of the above, the present invention provides a temperature controllable low temperature biological 3D print head apparatus that overcomes or at least partially solves the above problems.

In order to solve the technical problem, the invention provides a temperature-controllable low-temperature biological 3D printing nozzle device, which comprises:

a housing for mounting components;

a mounting block for mounting the syringe assembly; the mounting block is arranged inside the shell;

an injector assembly for injecting biological material; the injector assembly is arranged on the mounting block, and two ends of the injector assembly respectively extend out of the surface of the shell;

a fixing assembly for fixing the syringe assembly; the fixed component is arranged on the shell and is tightly propped against the injector component;

the air inlet pipe is used for inputting air into the injector assembly; the air inlet pipe is arranged on the fixing component and is connected with the injector component;

a heating membrane for heating the biological material in the syringe assembly; the heating film is arranged in the shell and is adjacent to the mounting block;

the refrigerating piece is used for refrigerating the biological materials in the injector assembly; the refrigerating sheet is arranged in the shell and is adjacent to the mounting block;

the heat dissipation assembly is used for dissipating heat of the refrigeration sheet; the heat dissipation assembly is arranged inside the shell, is adjacent to the refrigeration sheet and is connected with the outside of the shell.

Preferably, the mounting block is provided with a mounting hole penetrating through the mounting block, and the syringe assembly is arranged in the mounting hole.

Preferably, the syringe assembly comprises: the injection tube comprises a tube body, an injection head at the first end of the tube body and a fixing sheet at the second end of the tube body, the tube body is arranged in the mounting hole in the mounting block, the injection head extends out of the shell, and the fixing sheet is clamped between the fixing component and the shell.

Preferably, the syringe assembly further comprises: the piston is arranged in the cylinder and is in sealing connection with the inner wall of the cylinder.

Preferably, the fixing assembly comprises: the injection syringe comprises a pressing plate and a fixing screw, wherein a convex block is arranged on the shell, the fixing screw is screwed in the convex block and extends out of the convex block, and the pressing plate is clamped between the fixing screw and a fixing plate of an injection barrel in the injection syringe assembly.

Preferably, a fixed pipe is arranged on the pressure plate, the fixed pipe extends out of the surface of the pressure plate, a first end of the fixed pipe extends into the cylinder of the injection cylinder, and a second end of the fixed pipe is sleeved on the air inlet pipe.

Preferably, the pressing plate is provided with a reinforcing rib, and the reinforcing rib is connected with the fixing pipe and tightly abutted against the fixing screw.

Preferably, the heat dissipation assembly includes: and the cooling fan is arranged on the shell and is opposite to the refrigerating sheet.

Preferably, the heat dissipation assembly further comprises: and the radiating fin is arranged between the radiating fan and the refrigerating fin.

Preferably, a heat dissipation grid is arranged on the housing, and the heat dissipation grid is opposite to the heating film.

One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages: the temperature-controllable low-temperature biological 3D printing nozzle device provided by the application can adjust the temperature of a biological material as required, is not only beneficial to ensuring the performance consistency of the biological material, but also can be combined with an original low-temperature cooling platform to break through the layer height of low-temperature biological 3D printing, is beneficial to relaxing the requirements of a low-temperature 3D biological printing technology on the biological material, and enables more materials to have opportunities to be applied to the low-temperature biological 3D printing technology; meanwhile, the temperature-controllable spray head is adopted for low-temperature biological 3D printing, so that the scaffold which is higher in porosity and better in biocompatibility than the scaffold obtained by the original low-temperature biological 3D technology is expected to be obtained.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.

Fig. 1 is a schematic overall structure diagram of a temperature-controllable low-temperature biological 3D printing nozzle device according to an embodiment of the present invention;

FIG. 2 is an exploded view of a controllable temperature and low temperature biological 3D printing nozzle device according to an embodiment of the present invention;

fig. 3 is an exploded schematic view of a temperature-controllable low-temperature biological 3D printing nozzle device according to an embodiment of the present invention.

Detailed Description

The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.

Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is a conflict, the present specification will control.

Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.

Referring to fig. 1 to 3, in an embodiment of the present application, the present invention provides a temperature-controllable low-temperature biological 3D printing nozzle device, including:

a housing 10 for mounting various parts;

a mounting block 20 for mounting a syringe assembly 30; the mounting block 20 is disposed inside the housing 10;

an injector assembly 30 for injecting biological material; the syringe assembly 30 is arranged on the mounting block 20, and two ends of the syringe assembly respectively extend out of the surface of the shell 10;

a fixing assembly 40 for fixing the syringe assembly 30; the fixing component 40 is arranged on the shell 10 and is abutted against the injector component 30;

an inlet tube 50 for inputting gas into the injector assembly 30; the air inlet pipe 50 is arranged on the fixing component 40 and is connected with the injector component 30;

a heating membrane 60 for heating the biological material in the syringe assembly 30; the heating film 60 is disposed inside the housing 10 and adjacent to the mounting block 20;

a refrigeration sheet 70 for refrigerating the biological material in the injector assembly 30; the refrigeration sheet 70 is arranged inside the shell 10 and adjacent to the mounting block 20;

a heat dissipation assembly 80 for dissipating heat from the cooling fins 70; the heat dissipation assembly 80 is disposed inside the housing 10, adjacent to the refrigeration sheet 70, and externally connected to the housing 10.

In the embodiment of the present application, the syringe assembly 30 is filled with the biomaterial in advance, then the syringe assembly 30 is mounted on the mounting block 20 inside the housing 10, the syringe assembly 30 is firmly fixed on the housing 10 through the fixing assembly 40, then the air inlet tube 50 is mounted on the fixing assembly 40 and is connected with the inside of the syringe assembly 30, then the air inlet tube 50 is connected with the outside ventilation device, the housing 10 is moved to align the bottom end of the syringe assembly 30 with the printing platform, the ventilation device is opened to ventilate the inside of the syringe assembly 30, the air pressure inside the syringe assembly 30 is increased, and the biomaterial is dripped out of the syringe assembly 30 under the squeezing action of the air pressure. Meanwhile, the heating film 60 can be opened as required to heat and heat the biological material in the injector assembly 30, or the cooling plate 70 can be opened as required to cool the biological material in the injector assembly 30, and the heat dissipation assembly 80 can be opened as required to enhance the cooling effect of the cooling plate 70. The speed of the dripping of the biomaterial inside the syringe assembly 30 and the temperature of the biomaterial may be adjusted as desired.

In the embodiment of the present application, as shown in fig. 1 to 3, the mounting block 20 is provided with a mounting hole 21 penetrating therethrough, and the syringe assembly 30 is disposed in the mounting hole 21.

In the embodiment of the present application, the mounting block 20 is a rectangular parallelepiped and is mounted inside the housing 10, and a mounting hole 21 is formed therethrough for fastening the syringe assembly 30. Two ends of the housing 10 corresponding to the mounting blocks 20 are respectively opened for inserting the mounting blocks 20 into the housing 10 and completing the installation.

1-3, in the present embodiment, the syringe assembly 30 includes: the injection tube 31, the injection tube 31 includes a cylinder 32, and an injection head 33 at a first end and a fixing plate 34 at a second end of the cylinder 32, the cylinder 32 is disposed in the mounting hole 21 of the mounting block 20, the injection head 33 extends out of the housing 10, and the fixing plate 34 is clamped between the fixing component 40 and the housing 10.

In the embodiment of the present application, the cylinder 32 of the syringe 31 is fastened in the mounting hole 21 of the mounting block 20, the injection head 33 extends out of the mounting hole 21 and the housing 10, the fixing piece 34 is supported on the surface of the housing 10, and the fixing member 40 presses the fixing piece 34 against the surface of the housing 10, so that the syringe 31 is firmly mounted in the mounting block 20. The interior of the barrel 32 is used to store biological material. In the case of non-aeration, since the tube diameter of the injection head 33 is small and the biomaterial has a certain viscosity, the biomaterial can be stably held in the cylinder 32 without dripping out through the injection head 33. The injection head 33 may also be provided with a sealing plug, which can be taken out when printing is to be performed, and then plugged into the sealing plug after printing is completed.

As shown in fig. 1-3, in the present embodiment, the syringe assembly 30 further includes: and the piston 35 is arranged in the cylinder 32, and is in sealing connection with the inner wall of the cylinder 32.

In the embodiment of the present application, the piston 35 is made of rubber, has a diameter equal to the inner diameter of the cylinder 32, is tightly attached to the inner wall of the cylinder 32, and can reciprocate along the cylinder 32 under the action of air pressure.

As shown in fig. 1 to 3, in the embodiment of the present application, the fixing assembly 40 includes: the injection syringe comprises a pressing plate 41 and a fixing screw 42, wherein a projection 11 is arranged on the shell 10, the fixing screw 42 is screwed in the projection 11 and extends out of the projection 11, and the pressing plate 41 is clamped between the fixing screw 42 and a fixing plate 34 of an injection barrel 31 in the injection syringe assembly 30.

In the embodiment of the present application, the housing 10 is provided with a projection 11 for detachably mounting the fixing screw 42. After the injection tube 31 is inserted into the mounting hole 21, the pressing plate 41 is pressed against the fixing piece 34, and then the fixing screw 42 is screwed into the through hole of the projection 11 and pressed against the top wall of the pressing plate 41, so that the injection tube 31 can be firmly mounted in the mounting hole 21. There may be two projections 11, and the two projections 11 are oppositely disposed on the housing 10 for mounting the two fixing screws 42. The two fixing screws 42 are respectively abutted against two ends of the fixing piece 34, so that the syringe 31 is stably mounted in the mounting hole 21.

Referring to fig. 1 to 3, in the embodiment of the present application, a fixed tube 43 is disposed on the pressure plate 41, the fixed tube 43 extends out of the surface of the pressure plate 41, and has a first end extending into the cylinder 32 of the injection cylinder 31 and a second end sleeved on the air inlet tube 50.

In the embodiment of the present application, the fixing tube 43 extends into the cylinder 32, so as to prevent the injection tube 31 from shaking left and right, and the air inlet tube 50 extends into the fixing tube 43, so that the gas in the air inlet tube 50 can enter the injection tube 31 through the fixing tube 43.

As shown in fig. 1 to 3, in the embodiment of the present application, a reinforcing rib 44 is disposed on the pressing plate 41, and the reinforcing rib 44 is connected to the fixing tube 43 and tightly pressed against the fixing screw 42.

In the embodiment of the present application, the reinforcing rib 44 is triangular and connected to the fixing tube 43, and can be used to enhance the stability of the fixing tube 43 on the pressing plate 41.

As shown in fig. 1 to 3, in the embodiment of the present application, the heat dissipation assembly 80 includes: and the heat radiation fan 81 is arranged on the shell 10, and is opposite to the refrigeration sheet 70.

In the embodiment of the present application, the heat dissipation fan 81 is connected to an external power source, and can be turned on or off as needed.

As shown in fig. 1 to 3, in the embodiment of the present application, the heat dissipation assembly 80 further includes: and the heat radiating fin 82 is arranged between the heat radiating fan 81 and the refrigerating fin 70.

In the embodiment of the present application, the heat sink 82 is disposed between the heat dissipation fan 81 and the cooling fins 70, and may be used to enhance the effect of reducing the temperature of the cooling fins 70.

As shown in fig. 1 to 3, in the present embodiment, a heat dissipation grid 12 is disposed on the housing 10, and the heat dissipation grid 12 is opposite to the heating film 60.

In the embodiment of the present application, the heating film 60 generates a large amount of heat when heated, and the heat can flow out of the interior of the housing 10 through the heat dissipation grid 12, so as to avoid the influence of the high temperature inside the housing on the activity of the biomaterial.

The temperature-controllable low-temperature biological 3D printing nozzle device provided by the application can adjust the temperature of a biological material as required, is not only beneficial to ensuring the performance consistency of the biological material, but also can be combined with an original low-temperature cooling platform to break through the layer height of low-temperature biological 3D printing, is beneficial to relaxing the requirements of a low-temperature 3D biological printing technology on the biological material, and enables more materials to have opportunities to be applied to the low-temperature biological 3D printing technology; meanwhile, the temperature-controllable spray head is adopted for low-temperature biological 3D printing, so that the scaffold which is higher in porosity and better in biocompatibility than the scaffold obtained by the original low-temperature biological 3D technology is expected to be obtained.

It is noted that, in this document, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element. The above description is merely exemplary of the present application and is presented to enable those skilled in the art to understand and practice the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

In short, the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a biological 3D of controllable temperature low temperature prints shower nozzle device which characterized in that includes:

a housing for mounting components;

2. The temperature-controllable cryogenic organism 3D printing spray head device of claim 1 wherein the mounting block is provided with a mounting hole therethrough, the injector assembly being disposed in the mounting hole.

3. The controlled temperature cryogenic biological 3D printing showerhead apparatus of claim 1, wherein the injector assembly comprises: the injection tube comprises a tube body, an injection head at the first end of the tube body and a fixing sheet at the second end of the tube body, the tube body is arranged in the mounting hole in the mounting block, the injection head extends out of the shell, and the fixing sheet is clamped between the fixing component and the shell.

4. The controllable temperature cryogenic organism 3D print head apparatus of claim 3, the injector assembly further comprising: the piston is arranged in the cylinder and is in sealing connection with the inner wall of the cylinder.

5. The controllable temperature cryogenic organism 3D printing jet apparatus of claim 1, wherein the fixed assembly comprises: the injection syringe comprises a pressing plate and a fixing screw, wherein a convex block is arranged on the shell, the fixing screw is screwed in the convex block and extends out of the convex block, and the pressing plate is clamped between the fixing screw and a fixing plate of an injection barrel in the injection syringe assembly.

6. The temperature-controllable cryogenic organism 3D printing nozzle device according to claim 5, wherein a fixed tube is disposed on the pressure plate, the fixed tube extends out of the surface of the pressure plate, and has a first end extending into the barrel of the syringe and a second end sleeved on the air inlet tube.

7. The temperature-controllable low-temperature biological 3D printing nozzle device according to claim 6, wherein a reinforcing rib is arranged on the pressure plate, and the reinforcing rib is connected with the fixing pipe and tightly abutted against the fixing screw.

8. The controllable temperature cryogenic organism 3D printing showerhead apparatus of claim 1, wherein the heat sink assembly comprises: and the cooling fan is arranged on the shell and is opposite to the refrigerating sheet.

9. The controllable temperature cryogenic organism 3D printing showerhead apparatus of claim 8, wherein the heat sink assembly further comprises: and the radiating fin is arranged between the radiating fan and the refrigerating fin.

10. The temperature-controllable low-temperature biological 3D printing nozzle device according to claim 1, wherein a heat dissipation grid is arranged on the housing, and the heat dissipation grid is opposite to the heating film.