CN112890871A

CN112890871A - Exhaled breath condensate portable sampler and sampling method

Info

Publication number: CN112890871A
Application number: CN202110053316.7A
Authority: CN
Inventors: 要茂盛; 李心月
Original assignee: Peking University
Current assignee: Peking University
Priority date: 2021-01-15
Filing date: 2021-01-15
Publication date: 2021-06-04

Abstract

The invention discloses a portable expired gas condensate sampler and a sampling method, which utilize a semiconductor refrigeration sheet to provide a low-temperature condensation environment and use a sterile disposable suction tube and a 50ml centrifugal tube to collect the expired gas condensate of a human body. The sampler has simple structure, portable volume and simple operation, does not need other non-portable consumables and auxiliary equipment such as refrigerants and the like except a power supply, can noninvasively and rapidly collect enough human exhaled breath condensate for disease infection condition analysis, can avoid discomfort and cross infection of a tested person and sample cross pollution to the maximum extent, and is suitable for large-scale sampling detection diagnosis and epidemiological investigation of respiratory tract pathogen infected people.

Description

Exhaled breath condensate portable sampler and sampling method

Technical Field

The invention relates to a portable sampler for exhaled breath condensate and a sampling method, in particular to a portable sampler for exhaled breath of a human body and a sampling method, wherein the portable sampler integrates a rectangular semiconductor refrigeration sheet and cyclone air blowing.

Background

The airborne viral epidemic can often cause huge social panic and economic loss, and in order to prevent and control the viral spread and the epidemic spread, in addition to measures such as social isolation, the method more importantly utilizes large-scale viral infection detection to diagnose, isolate and treat patients with viral infection in time, and simultaneously takes appropriate measures to protect susceptible people. The detection means widely used at present mainly performs diagnosis based on a sample collection method such as throat swab, nasal swab, blood assay, etc., in combination with nucleic acid detection or immunological detection. These sample collection methods can cause discomfort to the subject, making it difficult for the patient to cooperate to collect an unacceptable sample, resulting in a possible false negative test result. In addition, the traditional throat swab and nasal swab methods can only collect biological samples of the upper respiratory tract and cannot detect infection occurring in the lower respiratory tract, so that false negative results are more likely to be obtained by throat swab detection for early lower respiratory tract infection.

The exhaled breath comes from the lower respiratory tract and contains physiological information such as infection of the lower respiratory tract and metabolic conditions of the human body. In recent years, exhaled breath has received much attention as a novel noninvasive diagnosis method. The exhaled breath research and diagnosis is performed by taking appropriate samples, and the main methods at present are the air bag method and the condensate method. The air pocket method results in loss of exhaled breath components over storage time due to component adhesion and wall effects. In order to ensure the condensation effect, the condensate method generally needs to be provided with larger refrigeration equipment, such as common commercial exhaled breath condensate collecting equipment EcoScreen, TurboDECCS, ANACON and the like, is complex to operate and is not easy to carry, and the application and popularization of the exhaled breath condensate in health research and disease diagnosis are limited. Furthermore, these devices have the potential to cross-contaminate to varying degrees. Therefore, there is a need for an exhaled breath condensate sampling device that is easy to operate and convenient to carry, and particularly for the diagnosis of respiratory tract infections, a new method that can collect exhaled breath condensate conveniently and quickly without cross contamination is needed.

Disclosure of Invention

The invention aims to provide a portable device and a corresponding sampling method for rapidly collecting the exhaled breath condensate of a human body by utilizing a semiconductor refrigeration sheet and cyclone exhalation based on a low-temperature condensation principle, and the condensation temperature is set by a temperature control system so as to rapidly collect the exhaled breath condensate under different environmental temperature conditions and different sample collection requirements, so that the portable device is particularly suitable for collecting the exhaled breath condensate for the purpose of diagnosing lower respiratory tract infection.

The main technical principle of the invention is as follows: referring to fig. 1, a semiconductor refrigerating sheet 6 is tightly attached to a cold coagulation block 7 coated with a heat insulation layer for refrigerating, and an exhaled air low-temperature condition is created for a condensation sampling tube 4 placed in the semiconductor refrigerating sheet. The testee uses the disposable suction tube 1 passing through the upper cover 2 of the sampling tube to exhale in a unidirectional cyclone manner into the condensation sampling tube 4, so that the exhaled air is directly contacted with the wall of the low-temperature tube and condensed into liquid drops to be collected. 200-.

The technical scheme of the invention is as follows:

a portable exhaled breath condensate sampler comprises a sampler shell and a condensation sampling tube, and is characterized in that a cold condensate block, a semiconductor refrigeration sheet, an air cooling radiator, a temperature sensing probe and a temperature control module are arranged in the sampler shell; the refrigerating surface of the semiconductor refrigerating sheet is tightly attached to the cold coagulation block, and the heat conducting surface is tightly attached to the air-cooled radiator; a hole matched with the shape of the condensation sampling pipe is formed in the middle of the condensation block and used for placing the condensation sampling pipe; the opening of the condensation sampling pipe is exposed out of the shell of the sampler and is covered by the upper cover of the sampling pipe, and the middle of the upper cover of the sampling pipe is provided with an inclined round hole for inserting a disposable straw; the temperature sensing probe detects the temperature of the cold coagulation block and transmits the temperature to the temperature control module; the semiconductor refrigerating sheet and the air-cooled radiator are electrically connected with the temperature control module and are regulated and controlled by the temperature control module; the display screen and the setting key of the temperature control module are exposed on the surface of the shell of the sampler.

In the portable exhaled breath condensate sampler, the semiconductor refrigeration sheet is preferably a rectangular thermal conductive refrigeration sheet, and a heat pump is formed to convey heat from one side to the other side when direct current passes through the semiconductor refrigeration sheet. The semiconductor refrigerating sheet is a refrigerating surface closely attached to the condensing block, the semiconductor refrigerating sheet is a heat conducting surface closely attached to the air cooling radiator, and preferably, the joint of the two surfaces is coated with a thin heat conducting silicone grease layer to ensure the heat conducting performance of the joint. Preferably, the specification of the semiconductor refrigerating sheet is (40 +/-20) mmX (4.2 +/-0.5) mm, the number of the substrate element pairs is more than 127, the maximum working current is 5 +/-3A, the rated voltage is 12-24V, the maximum temperature difference is more than 60 ℃, and the working environment is between-55 ℃ and 80 ℃. In the embodiment of the invention, the semiconductor chilling plates are 40mm multiplied by 4.2mm, the number of the substrate element pairs is 199, the maximum working current is 5A, and the rated voltage is 12V.

In the portable exhaled air condensate sampler, the air-cooled radiator is preferably a copper pipe air-cooled radiator and comprises a radiating copper plate, a hollow copper pipe, radiating fins and a radiating fan, wherein the hollow copper pipe penetrates through the radiating copper plate and is inserted into the radiating fins, the radiating fan is attached to the radiating fins, and the refrigerating surface of the semiconductor refrigerating piece is attached to the radiating copper plate. The copper pipe air-cooled radiator of the invention preferably has 4 to 8 hollow copper pipes passing through the radiating copper plate, and the specification of the radiating copper plate is (40 plus or minus 20) mmX (4 plus or minus 0.5) mm; the hollow copper pipe penetrates through the heat dissipation copper plate and then is inserted into the aluminum or copper alloy heat dissipation fin group, and a heat dissipation fan is installed on the outer side of the heat dissipation fin group for cooling. In the embodiment of the invention, the air-cooled radiator is provided with 4 hollow copper tubes, and the specification of the radiating copper plate is 40mm multiplied by 4 mm.

The heat dissipation fan is preferably a miniature large-air-volume fan with the air volume of 30-300 CFM and is fixed on the outer side of the heat dissipation fin group through a metal clamp. The size of the heat radiation fan is preferably in the range of (90 +/-10) mmX (30 +/-5) mm, the rated voltage is 7.0-24V, the rated current is 0.3-6A, and the air volume is 30-300 CFM. In the embodiment of the invention, the size of the heat radiation fan is 90mm multiplied by 33mm, the rated voltage is 12V, the rated current is 6A, and the air volume is 300 CFM.

In the portable exhaled breath condensate sampler, the temperature control module is preferably a digital temperature control module, and the semiconductor chilling plate can be automatically controlled to be powered on or powered off according to the preset target temperature and the temperature measurement result of the temperature sensing probe. The power supply voltage is 12V/24V/220V, the temperature measuring and controlling range is-50-80 ℃, the precision is 0.1 ℃, and the load power can be 120W/240W/1500W. The surface of the shell of the sampler is provided with 3 adjusting keys and a digital display screen accurate to 0.1 for setting the target temperature. In the embodiment of the invention, the rated power supply voltage of the digital temperature control module is 220V, the digital temperature control module is connected to 220V mains supply power through a three-phase plug, and the load power is 120W.

In the portable exhaled breath condensate sampler, the temperature sensing probe is preferably a thermocouple sensor connected to the digital temperature control module, and the end of the temperature sensing probe is a waterproof probe with a stainless steel metal shell.

In the portable exhaled breath condensate sampler, the condensation block is preferably a metal heat conduction block coated with a heat insulation layer, is made of copper or aluminum alloy, and has the specification of 40 +/-20 mm in length, 40 +/-20 mm in width and 120 +/-20 mm in height. In order to match the shape of a 50ml centrifuge tube (as a condensation sampling tube), a circular hole with a conical bottom with the diameter of 30 +/-2 mm and the height of 115 +/-5 mm is left in the middle of the metal heat conducting block. The outside of the metal heat conducting block except the contact part of the hole for placing the condensation collecting pipe and the semiconductor refrigerating sheet is completely wrapped and insulated by using a foamed plastic adhesive tape as a heat insulating layer. The temperature sensing probe is wrapped between the heat insulation layer and the metal heat conduction block. In the embodiment of the invention, the condensing block has the specification of 40mm multiplied by 120mm, the diameter of the middle round hole is 30mm, and the bottom is conical.

In the portable exhaled breath condensate sampler, the condensation sampling tube is preferably a 50ml centrifugal tube, which is made of glass, acrylic, organic resin and other materials, has a diameter of 30 +/-2 mm and a length of 115 +/-5 mm, and has an appearance consistent with that of a circular hole in a cold-coagulation block. In the embodiment of the invention, the condensation sampling tube is a 50ml polypropylene centrifuge tube with a diameter of 30mm, a length of 115 mm and a conical bottom.

In the above portable exhaled breath condensate sampler, the sampling tube upper cover is preferably a custom made centrifuge tube upper cover with an inclined circular hole and a parallel air outlet nozzle. The diameter of the inclined circular hole is 30 +/-2 mm, the thickness of the inclined circular hole is 10 +/-5 mm, and the diameter of the inclined circular hole is 5 +/-2 mm. The parallel air outlet nozzle is a circular hole at the center of the upper cover, the diameter of the parallel air outlet nozzle is 3 +/-1 mm, the outlet at the upper end of the parallel air outlet nozzle is a nozzle which is diffused into 4 outlets in the horizontal direction, and the diameter of each nozzle in the horizontal direction is 1 +/-0.5 mm. The upper cover of the sampling pipe is matched with the opening of the condensation sampling pipe but is not sealed. In the embodiment of the invention, the diameter of the upper cover of the sampling tube is 30mm, the thickness of the upper cover of the sampling tube is 10 mm, the diameter of the inclined round hole is 5mm, the diameter of the parallel air outlet nozzle is 3mm, and the diameter of the diffusion nozzle at the upper end of the upper cover of the sampling tube is 1 mm.

Generally, the disposable pipette inserted into the inclined circular hole of the upper cover of the sampling tube is a sterile independently packaged plastic pipette, and the material of the disposable pipette can be polyethylene or polypropylene, the diameter of the disposable pipette is 5 +/-2 mm, and the length of the disposable pipette is 200 +/-50 mm. The embodiment of the invention selects the polypropylene material, and the aseptic disposable plastic straw which is independently packaged and has the diameter of 5mm and the length of 200 mm is used.

In the portable exhaled breath condensate sampler, the shell of the sampler can be made of organic resin, metal and other materials, and a heat dissipation air outlet is formed in the shell. The size of the shell is 30 +/-5 cm in length, 20 +/-5 cm in width and 20 +/-5 cm in height. In the embodiment of the invention, the shell of the sampler is made of organic resin and has the size of 30mm multiplied by 20 mm.

When the exhaled breath condensate portable sampler is used for sampling exhaled breath, the method comprises the following steps: adjusting the preset temperature of the sampler to a specific temperature within the range of-5 ℃ to-10 ℃, putting the condensation sampling tube into the cold coagulation block, and covering the sampling tube with an upper cover; treat that condensation piece temperature reachs and predetermine the temperature, insert disposable straw in the condensation sampling pipe, the testee mouth contains disposable straw and exhales the collection, exhale the in-process and require the straw entry not to contact tongue and oral cavity wall in the testee oral cavity in order to avoid the saliva to get into, the testee only uses the nose to breathe in simultaneously, only exhale with the mouth, the air current unidirectional flow in the straw avoids inhaling the condensate back to in the straw, so one-way exhale a plurality of minutes (3 to 5 minutes) with the mouth, the sampling finishes.

The invention provides a portable expired gas condensate sampler and a sampling method, which utilize a semiconductor refrigeration sheet to provide a low-temperature condensation environment, and use a sterile disposable suction tube and a 50ml centrifugal tube to collect the expired gas condensate of a human body. The device has simple structure, portable volume and simple operation of the acquisition method, and does not need other non-portable consumables and auxiliary devices such as refrigerants and the like except an external power supply. Can collect the human body exhaled breath condensate which is enough for disease infection condition analysis noninvasively and rapidly, can avoid the discomfort and cross infection of the testee and the sample cross contamination to the maximum extent, and is suitable for the large-scale sampling detection diagnosis and epidemiological investigation of the respiratory tract pathogen infected crowd. The portable exhaled breath condensate sampler and the sampling method are mainly characterized in that:

(1) the collection expired air condensate pipeline is sterile disposable material, realizes quick noninvasive collection, and reduces the risk of discomfort of a testee and cross contamination of a sample to a great extent.

(2) Adopt the semiconductor refrigeration piece to provide the low temperature condensation environment, need not extra refrigerating fluid or refrigeration plant, and exhale and hug closely the pipe wall, realize quick effective condensation, refrigeration plant operation is stable and the light and handy simple structure of volume, the operation of being convenient for and carrying.

(3) The sample consumables only need a universal sterile disposable suction tube and a centrifuge tube, and the sampling cost is low; meanwhile, the whole machine has simple structure and lower accessory cost, and is beneficial to large-scale use in communities, hospitals and the like.

Drawings

FIG. 1 is a schematic structural view of the exhaled breath condensate portable sampler of the present invention, wherein: 1-disposable suction pipe, 2-sampling pipe upper cover, 3-temperature sensing probe, 4-condensation sampling pipe, 5-sampler shell, 6-semiconductor refrigerating sheet, 7-cold clot coated with heat insulation layer, 8-digital temperature control module, 9-temperature setting button, 10-external power line, 11-copper pipe air-cooled radiator and 12-radiating fan.

Detailed Description

The sampler and sampling method of the present invention will be described in further detail by embodiments with reference to the accompanying drawings.

As shown in fig. 1, the portable exhaled breath condensate sampler comprises a sampler shell 5, a condensation sampling tube 4 arranged in the sampler shell 5, a semiconductor refrigeration sheet 6, a condensation block 7 externally coated with a heat insulation layer, a temperature sensing probe 3, a digital temperature control module 8 and a copper tube air-cooling radiator 11. The refrigerating surface of the semiconductor refrigerating sheet 6 is tightly attached to the condensing block 7 externally coated with the heat insulation layer, the heat conducting surface is tightly attached to the copper pipe air-cooled radiator 11, and the joints of the two surfaces are coated with the heat conducting silicone grease thin layer to ensure the heat conducting performance of the joints. The copper pipe air-cooled radiator 11 is composed of a radiating copper plate, a hollow copper pipe, radiating fins and a radiating fan 12, wherein the hollow copper pipe penetrates through the radiating copper plate and is inserted into the radiating fins, and the radiating fan 12 is attached to the radiating fins. The condensation sampling pipe 4 is placed in a condensation block 7 externally covered with a heat insulation layer, and the opening of the condensation sampling pipe 4 is exposed out of the top of the sampler shell 5 and is sealed by a sampling pipe upper cover 2 matched with the condensation sampling pipe. Sampling pipe upper cover 2 is equipped with the slope round hole, and disposable straw 1 can pass this slope round hole and insert condensation sampling pipe 4 in, the middle part inner wall of the bottom contact condensation sampling pipe 4 of disposable straw 1. The temperature sensing probe 3 is coated inside the heat insulation layer of the condensation block 7 externally coated with the heat insulation layer and is tightly attached to the condensation block. The temperature sensing probe 3 is connected with the digital temperature control module 8 to provide a temperature measuring function. The semiconductor refrigerating sheet 6 and the heat radiation fan 12 are connected to the output end circuit of the digital temperature control module 8 through wires. The digital temperature control module 8 is fixed on the sampler shell 5, a digital display screen and a temperature setting key 9 of the digital temperature control module 8 are exposed on the surface of the sampler shell 5, and an external power line 10 connected to the digital temperature control module 8 penetrates through the sampler shell 5 to be connected with a power supply socket. The condensing block 7, the copper pipe air-cooled radiator 11 and the semiconductor refrigerating sheet 6 which are arranged inside the sampler and externally coated with the heat insulation layer are integrally fixed inside the sampler shell 5.

In the present embodiment, the semiconductor cooling plate 6 is a rectangular plate of 40mm × 40mm × 4.2mm, the number of substrate elements is 199, the maximum operating current is 5A, and the rated voltage is 12V. The copper pipe air-cooled radiator 11 is provided with 4 hollow copper pipes, and the specification of a radiating copper plate is 40mm multiplied by 4 mm. The size of the cooling fan 12 is 90mm × 90mm × 33mm, the rated voltage is 12V, the rated current is 6A, and the maximum air volume is 300 CFM. The rated power supply voltage of the digital temperature control module 8 is 220V, the temperature measurement and control range is-50-80 ℃, the precision is 0.1 ℃, and the load power is 120W. The condensing block 7 specification of the heat insulation layer that covers outside is 40mm x 120mm, establishes the round hole in the middle in order to place condensation sampling pipe 4, and the round hole diameter is 30 millimeters, and the bottom is the taper shape. The outer coating heat insulation layer of the cold clot is a foaming plastic adhesive tape which completely covers the cold clot. The condensation sampling tube 4 is a 50ml polypropylene centrifuge tube with a diameter of 30mm and a length of 115 mm, and the bottom of the condensation sampling tube is conical. The diameter of the sampling tube upper cover 2 is 30mm, the thickness is 10 mm, and the diameter of the inclined round hole is 5 mm; the diameter of the sampling pipe upper cover 2 parallel to the air outlet nozzle is 3mm, and the diameter of the diffusion nozzle at the upper end of the sampling pipe upper cover is 1 mm. The disposable straw 1 is made of polypropylene, has a diameter of 5mm and a length of 200 mm, and is a sterile independently packaged disposable plastic straw. The sampler case 5 is made of organic resin and has dimensions of 30cm × 20cm × 20 cm.

In the embodiment, the exhaled breath condensate is connected with a power supply for starting up 10 minutes in advance before sampling, the preset temperature is adjusted to-10 ℃, a condensation sampling pipe 4 is placed in a round hole of a condensation block 7 externally coated with a heat insulation layer, and a specially-made sampling pipe upper cover 2 is covered on an inlet of the sampling pipe. When the temperature of the condensation block 7 externally coated with the heat insulation layer reaches the preset temperature, the testee sits in front of the portable exhaled air condensate sampler, takes out the independently packaged sterile disposable suction tube 1 and inserts into the inclined round hole of the sampling tube upper cover 2, and the cyclone type exhalation can be realized. The mouth of a testee contains a disposable suction tube 1 to collect the expired air, the entrance of the suction tube is required to be not in contact with the tongue and the inner wall of the oral cavity of the testee in the expiration process so as to avoid the entrance of saliva, and meanwhile, the testee only inhales through the nose and exhales through the mouth. The air flow in the suction pipe flows in a single direction, so that condensate is prevented from being sucked back into the suction pipe, and the user can exhale for 3 to 5 minutes by using the mouth in a single direction. After sampling, the suction pipe is firstly drawn out from the upper part as medical waste to be properly collected and disposed, then the customized sampling pipe upper cover 2 is taken up, the centrifugal pipe upper cover of the original sampling pipe is well covered and screwed, and condensate is prevented from being leaked. After the cover is closed, the centrifuge tube is slightly thrown to observe whether the volume of the condensate is enough and whether saliva pollution exists. Samples were re-collected if there was too little volume or significant saliva contamination. If the volume of the condensate is sufficient and no saliva is polluted, the condensate in the 50mL sampling centrifuge tube is transferred to a 1.5mL sterile centrifuge tube by a pipette gun and is well preserved at low temperature. The exhaled breath condensate volume collected in this example was typically 200-. The condensate sample without saliva pollution is clear and bright, is in a water-like state and has no sticky feeling.

Claims

1. A portable exhaled breath condensate sampler comprises a sampler shell and a condensation sampling tube, and is characterized in that a cold condensate block, a semiconductor refrigeration sheet, an air cooling radiator, a temperature sensing probe and a temperature control module are arranged in the sampler shell; the refrigerating surface of the semiconductor refrigerating sheet is tightly attached to the cold coagulation block, and the heat conducting surface is tightly attached to the air-cooled radiator; a hole matched with the shape of the condensation sampling pipe is formed in the middle of the condensation block and used for placing the condensation sampling pipe; the opening of the condensation sampling pipe is exposed out of the shell of the sampler and is covered by the upper cover of the sampling pipe, and the middle of the upper cover of the sampling pipe is provided with an inclined round hole for inserting a disposable straw; the temperature sensing probe detects the temperature of the cold coagulation block and transmits the temperature to the temperature control module; the semiconductor refrigerating sheet and the air-cooled radiator are electrically connected with the temperature control module and are regulated and controlled by the temperature control module; the display screen and the setting key of the temperature control module are exposed on the surface of the shell of the sampler.

2. The portable exhaled breath condensate sampler of claim 1, wherein the connection between the cooling surface of the semiconductor cooling plate and the condensation block and the connection between the heat conducting surface and the air-cooled heat sink are coated with a thin layer of heat conducting silicone grease.

3. The portable exhaled breath condensate sampler of claim 1, wherein the semiconductor chilling plate is a rectangular thermoelectric chilling plate with a size of 40 ± 20mm x 4.2 ± 0.5mm, the number of pairs of base elements is greater than 127, the maximum operating current is 5 ± 3A, and the rated voltage is 12-24V.

4. The portable exhaled breath condensate sampler of claim 1, wherein the air-cooled heat sink is a copper tube air-cooled heat sink, and is composed of a heat-dissipating copper plate, a hollow copper tube, heat-dissipating fins, and a heat-dissipating fan, wherein the hollow copper tube passes through the heat-dissipating copper plate and is inserted into the heat-dissipating fins, and the heat-dissipating fan is attached to the heat-dissipating fins; the refrigerating surface of the semiconductor refrigerating sheet is tightly attached to the heat-radiating copper plate.

5. The portable exhaled breath condensate sampler of claim 4, wherein 4 to 8 hollow copper tubes in the copper tube air-cooled radiator pass through a heat-dissipating copper plate, the size of the heat-dissipating copper plate being 40 ± 20mm x 4 ± 0.5 mm; the hollow copper pipe penetrates through the heat dissipation copper plate and then is inserted into the aluminum or copper alloy heat dissipation fin group; the heat dissipation fan is a miniature large-air-volume fan with the air volume of 30-300 CFM.

6. The portable exhaled breath condensate sampler of claim 1, wherein the condensation block is a metal heat conducting block coated with a heat insulating layer, the metal heat conducting block is made of copper or aluminum alloy, and the exterior of the metal heat conducting block is completely wrapped by a foamed plastic tape as the heat insulating layer except for the contact part between the hole for placing the condensation collection tube and the semiconductor refrigeration sheet.

7. The portable exhaled breath condensate sampler of claim 1, wherein the temperature control module is a digital temperature control module with a temperature measurement and control range of-50 to 80 ℃ and an accuracy of 0.1 ℃; the temperature sensing probe is a thermocouple sensor connected to the digital temperature control module, and the tail end of the temperature sensing probe is a waterproof probe with a stainless steel metal shell.

8. The portable expired air condensate sampler of claim 1, wherein the condensation sampling tube is a 50ml centrifuge tube made of glass, acrylic or organic resin, and the bottom of the condensation sampling tube is conical.

9. The portable exhaled breath condensate sampler of claim 1 wherein the sampling tube is covered with an inclined circular aperture and a parallel air outlet nozzle.

10. A exhaled breath condensate sampling method, utilize any one of exhaled breath condensate portable sampler of claim 1-9 to sample, adjust the preset temperature of the sampler to a particular temperature within-5 duC to-10 duC at first, put the condensing sampling tube into cold clot, cover the sampling tube upper cover; inserting a disposable suction pipe into the condensation sampling pipe when the temperature of the condensation block reaches a preset temperature; the mouth of the testee contains a disposable straw to exhale and collect, the mouth of the testee requires that the straw inlet does not contact the tongue and the inner wall of the oral cavity in the oral cavity of the testee so as to avoid saliva from entering, meanwhile, the testee only uses the nose to inhale, only uses the mouth to exhale, airflow in the straw flows in a single direction, condensate is prevented from being sucked back into the straw, the mouth is used for exhaling for a plurality of minutes in a single direction, and sampling is finished.